Bambanker Direct Protocol
Contents
1. Serum free APPLICATIONS OKT4 mouse hybridoma HEK293 human embryonic kidney cell line HEK293T human embryonic kidney cell line HeLa human uterine cervical cancer cell line K562 human chronic myeloid leukemia cell line C2C12 mouse skeletal muscle cell line PC12 rat adrenal medulla tumor cell line STORAGE CONDITIONS Upon receipt store BAMBANKER DIRECT medium at 2 10 C in the dark in a constant temperature freezer When stored under these conditions and handled correctly BAMBANKER DIRECT can be used for 1 years after manufacture 2 BAMBANKER DIRECT Serum Free Cell Freezing Medium PRECAUTIONS Use only for research purposes not for human use This product contains bovine serum derived compounds Prior to using this medium perform the confirmation test on the cells under study The manufacturer is not responsible for any accident or damage caused by the use of this product fyou have any questions on the use of this medium please contact the local distributor STERILITY TEST Endotoxin Synthetic substrate method Mycoplasma PCR method Fungi and bacteria As per Japanese Pharmacopoeia ORDERING INFORMATION Product Cat No BAMBANKER DIRECT 1x 20 ml BBO7 CONTACT INFORMATION Nippon Genetics Europe GmbH Binsfelder Str 77 D 52351 D ren Phone 49 0 2421 2084690 Email info nippongenetics eu info nippongenetis de For more detailed product infor2. BAMBANKER DIRECT Serum Free Cell Freezing Medium Cat No BB07 BAMBANKER DIRECT 1x 20 ml STORAGE Store at 2 10 C in the dark BAMBANKER DIRECT is intended for research use only 2012 January Rev 02 BAMBANKER DIRECT Serum Free Cell Freezing Medium CONTENTS FEATURES aaeain E A A a 2 ABPLICATIONS mantsa e Aae 2 STORAGE CONDITIONS si acd PR E CHER p caedi Errea 2 PRECAUTIONS coc ied La Las tain atte opaco asas qaad 3 STERILITY TEST ferron a R Ri Au 3 ORDERING INFORMATION stt 3 CONTACT INFORMATION 3 PROTOCO xit ten n INI HUBOR IS UE ditat duas a Mela dus 4 FEATURES BAMBANKER DIRECT is a novel cell freezing medium which can be added directly to culture nedium without centrifuging the collected cells Then after suspending the cells the user can freeze and preserve the cells in a deep freezer at 80 C In order to meet customers requests this product has been modified not to require tiring preparation steps for freezing This newly developed medium maximizes the work efficiency significantly BAMBANKER DIRECT is manufactured by LYMPHOTEC Inc 5 26 9 Floral Building Hakusan Bunkyo Ku Tokyo 112 0001 Japan Tiring preparation steps for freezing are not necessary No programmable freezer is required Add BAMBANKER DIRECT to culture medium without centrifuging the cells Ready to use without dilution Rapid and long term freezing and preservation in a deep freezer 80
3. mation contact details questions or trouble shooting please visit our English website www nippongenetics eu or our German website www nippongenetics de BAMBANKER DIRECT Serum Free Cell Freezing Medium PROTOCOL BAMBANKER DIRECT Freezing of suspended cells Take out cells in a culture process from an incubator Measure the same amount of BAMBANKER DIRECT as that of the culture medium Measure the same amount of BAMBANKER DIRECT as that of the culture medium Add BAMBANKER DIRECT to the cells Conduct a gentle pipetting so that the culture medium and BAMBANKER DIRECT are uniformly mixed Transfer the mixed solution to cryo tubes for freezing and preservation Freeze the cells at 80 C Freezing of adherent cells Take out cells in a culture process from an incubator Remove the culture medium Rinse the cells twice with PBS Then add cell detachement solution and heat it up Add the same amount of BAMBANKER DIRECT as that of the detachement solution Conduct a gentle pipetting so that the detachement solution and BAMBANKER DIRECT are uniformly mixed Transfer the mixed solution to cryo tubes for freezing and preservation Freeze the cells at 80 C Thawing Rapidly thaw the cells at 37 C Suspend the cells in culture medium Centrifuge the cells and then remove the supernatant Suspend the cells in cult
4. ure medium and then start the seeding and cell culture
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