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MOOR INSTRUMENTS LIMITED Moorsoft for Windows for moorLAB

1. A E A AS 12 4 2 KEVIEW WINDOW a a ERROR a e e o a a ereen Tele 17 4 2 1 Open a Review WINDOW A td I7 4 2 2 ICON FL ONS cus Lot A iren m il E O E dee et 18 2 Moor Instruments Limited assumes no responsibility for the operator s failure to comply with instructions given in this manual 1 USING THE moorLAB USER MANUAL This manual is for use with Moor Instruments Moorsoft for Windows for moorLAB floLAB software run on an IBM 486 PC Pentium or compatible in conjunction with the moorLAB floLAB blood flow monitor Users unfamiliar with laser blood flow monitoring and analysis should read the Introduction of this manual Otherwise users need only read the section Step by Step Guide 2 NEW FEATURES COMPARED WITH V1 0 This version a b Does not require a dongle key to operate see Section 3 3 3 Registration Number Can display graphically either conc or dc values and stored values of either conc or dc Version 1 0 did not allow display and reading of dc oe un ee ee ee UM UR ED ee m Um um 3 INTRODUCTION 3 1 Laser Doppler Blood Flow Measurement Laser Doppler flowmetry provides easy to use non invasive real time measurements of local tissue blood flow Laser Doppler blood flow measurement uses the Doppler frequency broadening of laser light when the light is reflected off a moving object The degree of broardening is dependent on the speed of the moving object the wavelength of the laser light and the angle of
2. a ELE THES ETE I Please refer to section 3 1 3 and section 3 1 4 for configuration setup 17 4 2 2 Icon Functions The icon functions in a review window are the same as in a monitor window refer to pur TAO FIRES section 3 1 6 except that the icons B SER and are not available in a review window 18 e as 1 l l i Rr J Heo 3 A CE EZ AA EJ ES ed a mu A M M vn Moor Instruments Ltd Millwey Axminster Devon EX13 5HU England Telephone 44 0 1297 35715 Fax 44 0 1297 35716 E mail mail amp moor co uk Internet http www moor co uk Moor Instruments Inc 501 Silverside Road Suite 66 Wilmington DE 19809 USA Telephone 302 798 7470 Fax 302 798 7299 E mail moorincGinterserv com
3. ee h mambong ho ttibiote tt tt et e e tE ert y a m L a T B ny h E A 10 ub Y df NE EA utt sents 7 Dr WE A e E H 3 gt ee 5 7 pra A A us 1 r1 Tu HH rl SENI s M t In H E qaga err a v TIH e Hyn n E Te ONT Tp Te MER ATUM tal RST ab P t re preti 9 eo 4 av in which the graph link status active graph graph labels graph units time scale and time offset can be changed The parameter traces which will be displayed in each graph can be defined by ticking the corresponding check box button Upto 4 parameters can be assigned to each graph If more than 4 parameters are chosen the first 4 will be used The time constants Y offsets and Y scales can be configured by clicking the Y and TC button in the above window The following dialogue box will be shown to allow user to change the settings 11 m hia e E Ben EDDA a XE Ert us pe elis n 1 rdi ENS j iganta istis Hin ja St tht s Hi hi B UE ta 3 Hz Du uum a si RES Eee ee Hcc und E ol rete ae Ir j a A 4 1 4 Configuration file The configurations described in sections 3 1 2 and 3 1 3 can be saved into a file Configuration gt Config File gt Save so that user can use the same configuration next time by loading the config file Configuration gt Config File gt Load 4 1 5 Start a Measurement Once a monitor
4. the scatter For example laser light wavelength 780nm back scattered off a particle moving in a watery medium with a speed of Imm sec has a frequency shift of approximately 3 3K Hz Laser light is used to illuminate the skin tissue resulting in some of the light being reflected straight back by the static tissue and some being reflected by moving blood The reflected light from the static tissue and the frequency broadened light from moving blood is collected and mixed on a photodetector The photocurrent can then be processed to produce indications of the flux concentration and speed of the moving blood The moorLAB floLAB uses solid state laser diodes as the laser light sources at about 780nm Two glass fibres are used mounted in a probe head one to transmit the laser light to illuminate the tissue and the other fibre to collect the reflected light This is then photodetected The signal from the photodetector is amplified and processed by an analogue processor The signal is then sampled and further processed by a digital processor The digital processor also performs all the user interface and display functions The mean blood cell flux flux number concentration of moving blood cells conc mean speed of the blood speed of each moorLAB floLAB channel are output to a PC in real time via an RS232 senal link If moorLAB floLAB is running on a PC this information may be collected displayed stored analysed and converted into moorLAB
5. CEN A dm 4 I a Dx aa TREES MOOR INSTRUMENTS LIMITED Moorsoft for Windows for moorLAB V1 1 USER MANUAL Issue 1 A data handling program for use with the moorL AB 25th January 2000 Moor Instruments Limited Millwey Rise AXMINSTER Devon EX13 5HU England Tel 44 0 1297 35715 Fax 44 0 1297 35716 Website www moor co uk Email marketing moor co uk A EII ma m l 2 3 4 USING THE MOORLAB USER MANUAL asssssssssossossssecosssceosososasosossossesssesoasoraerosesesesaevosesceeeoseesees 3 NEW FEATURES COMPARED WITH V1 0 eere eee ee enero n neenon eaae erre e ea sorsenoereorsensseeonsesenonseree 4 INTRODUCCION H r Dees 5 3 1 LASER DOPPLER BLOOD FLOW MEASUREMENT csccesestececcesccvcescecsuccescevenesecsscuctaussaeeescrssarenesens 5 32 OVERVIEW OF MOORL AB ui is sani os Eco uU eeu wanda au ob aS Au pese Nea ULIS 7 3 3 THE COMPUTER AND SOFTWARE INSTALLATION cccccccccccsssossccccsscersvsveecessescrensuveccesseeuncsessunensesens 7 23 Computer OPENS AA EOS 7 332 Sofware InstallatioB A neen ben 7 3 3 3 ReguwIFaton NUDE ds esed abo dd 8 STEP BY STEP GUIDE ica 9 4 MONITORING WINDOW a dd E o e ELA eo P LR aren LL RI Uer 9 4 1 1 Open a Monitor Window sse E E 9 412 De Up Monitor Conor alion A A AS A O TAS 10 4 1 3 Seiun Display CONCURSO 11 4 1 4 Confteuration JIE esos AA A A qutd Mosen nacre med 12 4 1 5 Slat a A a RN E AN 12 4 1 6 LOON F UHCHDHS AAA
6. TT x m Once the region has been defined clicking the statistics icon will bring up an option dialogue box so that user can choose between calculating the statistics for all selected parameters raw data only and calculating statistics for parameters in the active graph only time constant considered If the first option is chosen the statistics will be shown as follows 13 TT ae 32 wire rites it Gtau ae ENT T 4 MA crample min StSushes ore uem a H ERRE AR x se Eee Viv VIP ET gt NS F m RES EEn EEN eat ra BREE ree za UR US ieee nie ts AE RIA Rep IE IOS Fi j 2450 301 164 292 249 Ct 2030 36 194 210 203 ESL 598 70 4 n amp F2 j 1521 187 115 192 155 C2 1125 38 105 120 112 80 S2 669 70_ EPS ESAS 02 C3 927 66 253 522 1 my EC M F4 965 101 L 4 635 15 53 2 zZz 85 7 T EE Bb 4 j 751 72 62 90 76 53 ER 85 3 7 61 62 Total Point 86 Start pomt 24sec End point 4 5 sec TC OFF If the second option 1s selected the following table will be given as graph 1 is the selected active graph e mlb Statistics 2 Ee ee oy i a 3H PRR rn A B TL XT Er n E dh HO e dh ure vu ISI Tier uH yee Sette are PERN rite ME EN T Aha Ser Fi j 2450 301 184 292 249 Ct 2030 S1 538 70 4 YI E NEMZE AUC NEN EMEN SUPR NETOIE T
7. e only the free run and preset time modes are implemented others are greyed out 10 ESTEE eo YU 4 1 3 Setup Display Configuration Once a monitoring window is open the display ES can be defined by selecting BH Configuration gt Display Setup or clicking the icon EEF The following window will be available Settings forgraphic display H t i oH ptione pito R E tro pe ppc derit PRESO ERE MN EH UH e t hie dv p te tr miim bh aad m qoe P ur der s dime m varum mas mem s m uem nal mum dr ene Mr Ar er WU Pe te gt DUE ER FHF AE inst H gt x ee M EENES fpe Traum P XM ARS T a Es gt x E KA A d gt x A h L Lj wa gt do J x H P t E T a a A Y h z 4 p X E n br 27 LY rra zu Cp 7 J rr A od of P A 2 H so o how J D EA d v EH B E tl qu v ve d Dar n a 1 1 Des Maud Mi eei dc J HE d H Iri E E a REM o E oy M Ye MT Es Z CEN MH n Te F 53 x y H bif am x DT en i z niger hpa 7 gt ae Es Pes ed Les p DE TH B E Ti MS 7 5 H d PM 4 n HP a y Ei PELIS PH P ER DIES X gt he cry a pete ast Mte Ufa SE a ar j he dex a A y H 2 P esi REIS Y yt z de T PITE F T d T Ar T AP AS T x H ait FES he 3 gt EL y E THES b AS 4 t 3 M h UNA E PTT pE T1 a f E 2 m Hs H beta J G def ES fier Het nr eri OAT La nl sare kt T nr bx gt EIS y da dose ear snee ijn al 5 metha span A wapent
8. format or text format The algorithms used to compute flux conc and speed are as follows kl Pdo dark short noise ux f de k2 P w dw dark short noise conc dc speed k3xflux conc is the frequency of Doppler shift 2xf f frequency in Hz P w is the power of signal at frequency o dc is the intensity of all detected light Q1 is the low cut off frequency 01 21f1 fj frequency in Hz 05 is the high cut off frequency 07 216 f gt frequency in Hz kj k and k3 are scaling constants The optical units are arbitrary The values assigned are determined by standardising the moorLAB floLAB parameter of flux blood flow etc using a physical standard e g the thermal Brownian motion of polystyrene microspheres sub micron diameter sphere in water The laser Doppler monitor user guide should be consulted for information on calibration 32 Overview of moorLAB The moorLAB program receives the moorLAB floLAB real time flux and conc data via the RS232 serial link and converts the information into a graphical format The data will be displayed in real time and stored The moorLAB software consists of two different windows they are Monitoring window receive and display real time parameter signals from moorLAB floLAB Review window load and analyse saved parameter data from a moorLAB format file 33 The Computer and Software Installation 3 3 1 Computer Specifications Only co
9. ing window is open and the moorLAB is in the RUN modea __ measurement can be started by selecting Monitor Start or clicking the icon EA measurement can be Paused Continued by toggling the icon LEE Ei At the end of a measurement selecting Monitor gt Abort or clicking the icon 222 STOR will stop the measurement The recorded data can be saved by selecting File gt SaveAs or clicking the ES icon E There are two file formats available moorLAB format and text format 4 1 6 Icon Functions The operations of individual elements of the icon tool bar are described below 1 23 setup monitor configuration 12 nm me HEE EB Ee EB HE FEE EE EE oo um a EB EB HE HE GN AEE HB setup display configuration x E3 52 calculate statistics within a selected region To define a statistics region press down the left mouse button at the beginning of the region followed by dragging the mouse with the left button still pressed to reach the end of the region and releasing the mouse button The selected region will be shown in the inverted colours as illustrated below Eye lt k gis i st jag tt Sere atl et SERES oe RT ne CL Et tees memes if Dane Hm Sm um vm vasi Elie S ped slette q EDS VE irr UN A O EPI Be andar rele E cat See esci di ROM EA ro A Ee cit ci p to ri Er Kt AS EI PUE a em f A ES pny PA Dio pcs m o ES re ri AF hn we A this ae MAS Boe eo D vor m XS E00 RII BI Rea ss TITLE T Dy m S P 4 a
10. mputers complying with IEC 950 should be used The minimum requirements for running the moorLAB software is Processor 486 dx PC Pentium 1s recommended Memory 4Mb RAM 50Mb space on hard disk drive depending on monitoring duration minimum recommended Operating system Windows 3 11 or higher e g Windows 95 3 3 2 Software Installation A working knowledge of Windows is assumed for installation and operation of moorLAB software To install place the disk in the drive A From Program Manager select File then Run Type A INSTALL Then follow the instructions 3 3 3 Registration Number A registration number has been assigned to the copy of moorLAB that has been supplied to you This number is automatically recorded at installation and can be displayed during RUN Click File New gt Configuration REA moorLAB Laser teres Perfusion Mo Do not change this number If it is accidentally changed you will need to re enter it Contact Moor Instruments if help is needed Note if you are using a demonstration version this is fully operational but is a time limited version usually 3 months A message will be displayed on expiry Cannot Monitor Program has passed its expiry date use Configuration Registration Number to enter new registration number Contact Moor Instruments if you wish to purchase the software Moor Instruments will supply a registration number on receipt of your order Setti
11. n link of the graphs If L is displayed in the rectangle all graphs are linked 1 e changing Y scale Y offset and time constant will affect all graphs If U is displayed in the rectangle all graphs are not linked i e changing Y scale Y offset and time constant will only affect the active graph link status active but ERU E Sean EE m juss EZE KBE MEUS pa PLE x EE S IM Tm lee ji 1 X scroll bar Y scroll bar 18 Active graph clicking on the numbered rectangle or on the graph rectangle will make that graph as the active graph 19 lt lt make the previous mark left to the current active mark as the active man 20 gt gt make the next mark right to the current active mark as the active mark 21 X scroll bar it can be used to enang time offset Any X scroll bar action will affect all graphs 22 Y scroll bar it can be used to native Y offset If all graphs are linked any Y scroll bar action will affect all graphs otherwise it will only affect the active graph 16 4 2 Review Window 4 2 1 Open a Review Window Data stored in the moorLAB format can be loaded into a review window To open a review window select File Open or click the icon 23 If the file is successfully opened the parameter traces will be displayed in the same configuration as when they were saved An example is illustrated below iE IU TASCHE S am Que XT x d 26 bs oe CER x 5 Tree Us s c Y E amp ler pm
12. ng Up Registration Number e um e 4 STEP BY STEP GUIDE 4 1 Monitoring Window 4 1 1 Open a Monitor Window To open a Monitoring window select File New or click the icon Es A monitoring window illustrated below will be shown Gra a i EBS S SPI t 8 M ee NS Rien Sn id qn Pate Aol Heir Be omg sys a str wipe i Read pik dee be end ch LA DOSE gos Sod el Cater Ae ces 7 Or PETS RATES A SER ue g x n by E P br UL d L4 v Rips 2 n 3 Ex E KE S i r wt OR 33 pu s B Anal isis Marks pL dut AI pilo CET ms etel uz rt d AT SE Ud i 1 gt NAAA YT NM x px et eee igs iz A H f i TAs P dl r t anm 5 ey aos I P T A fil ES D 1 Eu r r En vy H 4 ad T 2A Lee Bae a as Ed Vtg AAA aman jn son ten Byrd en RUE a A 4 1 20 Setup Monitor Configuration To setup monitor configuration select Configuration Monitor Setup or click the icon EEI The following window will appear t E Is EE iet dE pis BE Af T TH ES 320 pt mor Ur tief Fo ar rds Pt Due f Eger Gite U ap seers AAA Tee T ee tal y 5 Ye ik 22 DARE In the above window the serial port scroll slide display mode monitoring schedule and the display of de or conc can be selected Note that for some computers a delay in the graphic display can be observed in scroll mode If this occurs slide mode should be used Note that in this version of moorLAB softwar
13. otal Point 86 Start point 2 4 sec Endpoint 4 5 sec Active Graph Number 1 TC off 14 In both situations a File menu will be available to allow user to save or print the results 4 2 start a measurement 5 ES stop a measurement 6 ts toggle pause continue a measurement 7 4 expand time scale 8 ke compress time scale 9 d expand Y scale 10 HE compress Y scale 11 ee increase time constant smooth 12 re decrease time constant 13 LES insert a mark In a real time monitoring mode when the mark icon 1s clicked a mark will be added in real time The added mark will be defined as the active mark which is displayed by a black rectangle in the mark bar The mark label can be entered in the mark label Edit refer to the diagram on next page If real time monitoring is aborted or paused clicking the insert mark icon will add a mark in the cursor position and open an edit mark notes dialogue box so that user can enter mark label and comment as illustrated below Mark Notes i a M as rx f hu Mr Pire AY So uer leac pu enin voy AN RA a es pre me mert tet k es AAA b 14 ud delete the current active mark 15 ed print the parameter traces displayed in the current window after a measurement 16 EA open the help menu The commands help gives explanations for all menu items The Toolbar help explains all icon functions 15 17 Link status clicking the link status rectangle will toggle between link u

MOOR INSTRUMENTS LIMITED Moorsoft for Windows for moorLAB

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