UV/Visible Spectrophotometers
Contents
1. For more information visit www gelifesciences com f 1 wozoydop ds 12 639 Spectrophotometry 12 640 UV Visible Spectrophotometers Pharmacopoiea Compliant Spectrophotometers Ultrospec 5300 pro UV Visible Spectrophotometer PC controlled instrument fulfilling Pharmacopoeia requirements Shown in Gold color option e Meets the demanding requirements of pharmacopoeia standards in terms of instrument specification GLP self diagnostics and audit trail SWIFT II Applications Software e Bandwidth of 1 0 nm and excellent scanning capability e Press to read high performance deuterium and tungsten lamps for reduced cost of ownership e Supplied with SWIFT II METHOD application software e Supplied with an 8 position cell changer and serial interface cable e Uses minimal bench space and optional support provides space for a laptop PC Ultrospec 5300 pro is a high specification product with a bandwidth of 1 0 nm that meets pharmacopoeia resolution tests Its GLP self diagnostics mean it can be easily checked against published specifications An informative print out is available for record keeping A Qualification and Performance Verification Logbook is available so that key instrument specification parameters can be checked as a function of time essential in the pharmaceutical and drug discovery industries SWIFT II Applications Software supplied with the instrument provides modules for waveleng2. 2109 09 Printer stand 1 80 2109 96 Tungsten Halogen lamp vertical filament 1 80 2106 16 Tungsten Halogen Lamp Vertical Filament 1 80 2022 94 Deuterium Lamp Assembly Includes W lamp U1100 pro 1 80 2109 11 Only Interface for chart recorder 1 80 2109 03 Spreadsheet interface software 1 80 2109 02 Parallel printer cable 1 80 2071 87 Dust cover 1 80 2109 13 For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details Related Products Refer To SWIFT 1000 Applications Software page 654 UV Visible Cells page 651 Accessories for Spectrophotometers page 652 Wavelength range 200 900 nm Bandwidth 5 nm Absorbance range 0 300 to 3 000 Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LVdirective EN 61010 1 IEC1010 1 Test tube holder For more information visit www gelifesciences com f 1 wozoydop ds 12 643 1 Spectrophotometry NO 644 UV Visible Spectrophotometers Variable Wavelength Spectrophotometers NanoVue Spectrophotometer NEW e For the reliable measurement of nucleic acid and protein samples e Eliminates the need for cuvettes capillaries or other sample devices simply drop and read e Assays low volumes of 0 5 ul to 5 ul with accuracy thus conserving precious samples e Predefined methods for nucleic acid and protein quantitation and pr
3. Standard rectangular cell with lid 50 mm pathlength 10 mm i d 10 ml working volume 52 5 x 45 mm external dimensions 1 80 2002 63 Semi micro cell with lid and black walls 10 mm pathlength 4 mm i d 800 ul working volume 12 5 x 45 mm external dimensions 1 80 2002 77 set of 2 matched 80 2100 13 set of 8 matched 80 2109 82 Micro cell with lid and black walls 10 mm pathlength 2 mm i d 400 ul working volume 12 5 x 45 mm external dimensions 1 80 2002 95 set of 2 matched 80 2100 25 set of 8 matched 80 2109 83 Standard rectangular cell with stopper 10 mm pathlength 10 mm i d 2000 ul working volume 12 5 x 48 mm external dimensions 1 80 2002 70 Semi micro cell with stopper and black walls 10 mm pathlength 4 mm i d 800 pl working volume 12 5 x 48 mm external dimensions 1 80 2002 81 set of 2 matched 80 2100 22 Micro cell with stopper and black walls 10 mm pathlength 2 mm i d 400 ul working volume 12 5 x 48 mm external dimensions 1 80 2002 99 Microvolume Cells Capillary cell 0 5 mm pathlength 3 ul working volume UV grade silica Includes 100 quartz capillaries and Cristaseal 1 80 2104 66 Capillary cell 0 5 mm pathlength 3 ul working volume UV grade silica Includes 100 quartz capillaries and Cristaseal 1 80 2120 19 Ultra microvolume cell with black walls 5 mm pathlength 5 7 ul working volume 12 5 x 48 mm external dimensions UV grade silica 1 80 2103 68 Microvolume cell with black walls 10 mm pathlength 50 pl working vol
4. UV Visible Spectrophotometers Variable Wavelength Spectrophotometers Molecular Biology GeneQuant 1300 Spectrophotometer Easy to use with a large graphics display and flexible with wavelength scanning plus a large range of nucleic acid purification protein assays CyDye and cell culture density e Predefined methods for nucleic acid purification protein assays CyDye and cell culture density measurements e Wavelength scanning of nucleic acid samples for purity checks e Storage for 90 user defined methods with optional password protection e Large easy to read graphical display e Optional built in printer or output data via USB to a PC for printing or storage e Wireless bluetooth connectivity optional e Optics with no moving parts for high energy throughput combined with a Xenon lamp for a long lifetime e Three year lamp warranty GeneQuant 1300 has a wide range of pre stored methods for DNA RNA and oligonucleotide purity and concentration measurements Visual inspection of a nucleic acid scan can quickly alert you to the presence of impurities this is especially useful for RNA samples GeneQuant 1300 is suitable for determining the concentration in ug ml ng ul ug ul pmol ul and pmol and purity of nucleic acids after hybridization PCR and plasmid DNA minipreps GeneQuant 1300 can correct for differences in cell pathlengths and dilution factors Oligonucleotide primers can be characterized by keying in
5. Visible Cells Product Quantity Code Number Glass Standard rectangular cell with lid 1 mm pathlength 10 mm internal width 200 ul working volume 12 5 x 45 mm external dimensions 1 80 2003 83 Semi micro cell with lid and black walls 10 mm pathlength 4 mm internal width 800 ul working volume 12 5 x 45 mm external dimensions 1 80 2004 15 Standard rectangular cell with lid 5 mm pathlength 10 mm internal width 1 ml working volume 12 5 x 45 mm external dimensions 1 80 2003 85 Standard rectangular cell with lid 10 mm pathlength 10 mm internal width 2 ml working volume 12 5 x 45 mm external dimensions 1 80 2003 87 set of 8 matched 80 2109 81 Standard rectangular cell with lid 50 mm pathlength 10 mm internal width 10 ml working volume 52 5 x 45 mm external dimensions 1 80 2003 93 Standard rectangular cell with stopper 10 mm pathlength 10 mm internal width 2000 ul working volume 12 5 x 48 mm external dimensions 1 80 2003 98 Disposable Disposable Cells 10 mm pathlength minimum volume 1 5 ml Polystyrene pack of 100 1 80 2084 11 Disposable Cells 10 mm pathlength minimum volume 2 5 ml Methacrylate pack of 100 1 80 2004 53 For pricing information visit www gelifesciences com orderonline These cells are supplied with packing pieces to facilitate use in a standard 10 mm pathlength cell holder t Availability varies by region please contact your local GE Healthcare office for details Other Spectrophotometer Cells an
6. a capillary cell system A wide range of standard cuvettes may be used depending on the sensitivity or volume requirements The 15mm optical beam height makes the GeneQuant 100 compatible with low volume quartz and disposable UV transmitting plastic cuvettes Software and User interface Relevant parameters are calculated automatically and displayed at the touch of a button on a large display 646 For more information visit www gelifesciences com GE Healthcare Visible Soectrophotometers Variable Wavelength Spectrophotometers Ultrospec 500 pro Visible Spectrophotometer Ideal for the biological science teaching laboratory e Basic measurement modes coupled with enhanced software functionality and method storage Graphics for scan kinetics and standard curve Robust compact reliable and very easy to use Suitable for the biological science teaching laboratory Available in a choice of four colors Ultrospec 500 pro is a visible only spectrophotometer that addresses the needs of the biological science teaching laboratory The instrument has absorbance percentage transmission and factor concentration modes Absorbance against time plots and scans are shown on the graphics display there is also output directly to printer soreadsheet or chart recorder The benefits of enhanced software functionality including standard curve mode reaction rate slope calculation multi wavelength equation definition and method stor
7. the base sequence up to 66mer to obtain a conversion factor ug ml molecular weight theoretical absorbance AU umoll and theoretical Tm For licensing information see back of catalog GE Healthcare Supported by ORDERING INFORMATION Product Quantity Code Number GeneQuant 1300 Classic NEW 1 28 9182 13 GeneQuant 1300 Classic with printer NEW 1 28 9182 14 GeneQuant 1300 Classic with bluetooth NEW 1 28 9182 15 Accessories and other items for GeneQuant 1300 Bluetooth Accessory for GeneQuant 1300 Classic NEW 1 28 9182 25 Spare printer paper 20 rolls NEW 1 28 9182 26 GeneQuant 1300 Printer Accessory NEW 1 28 9182 27 For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details Not for sale in Canada Related Products UV Visible Cells Refer To page 651 Wavelength range 190 1100nm Bandwidth 5 nm Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LVdirective EN 61010 1 IEC1010 1 Lith Sacha off eff eff k west fika Teak al ma Dinner ikk of of of fi Te Dai kapuni cy iya PO B90 aa 6 500 mQ ial I Llais ete me gem im pa Ha aa ma on Windows style software makes application selection easy Display shows a spectral scan of a nucleic acid sample Proteins can be quantitated by BCA Biuret Bradford Direct UV and Lo
8. 8 Water heated cell holder 10 40 mm pathlength circulation bath required 1 80 2109 06 Peltier heated cell holder 20 50 C 10 mm pathlength powered from instrument 1 80 2106 13 Programmable Peltier unit 20 105 C and SWIFT T Applications software requires Temperature Control Unit and PC with serial port 1 80 2106 14 Electrically heated cell holder 25 30 37 C 10 mm pathlength powered from instrument 1 80 2106 12 Spare Single Cell Holder U500 U1100 pro 1 80 2109 09 Specialized Cell Holders Cell holder with 8 ul HPLC flow cell 1 80 2106 11 Cell holder 10 50 mm pathlength 1 80 2106 07 Cell holder 100 mm pathlength 1 80 2107 14 Cylindrical cell holder 25 mm diameter up to 100 mm pathlength 1 80 2106 10 Test tube holder accommodates diameters of 8 25 mm and heights of up to 180 mm 1 80 2109 33 For pricing information visit www gelifesciences com orderonline 652 For more information visit www gelifesciences com GE Healthcare Accessories for Spectrophotometers Cell Density Meter System Control Software Cell Changers Product Quantity Code Number 4 position cell changer 10 50 mm pathlength 1 80 2106 01 Spare 8 position cell changer 10 mm pathlength 1 80 2108 01 6 position Peltier heated cell changer 10 mm pathlength 1 80 2106 04 requires Temperature Control Unit see 80 2105 49 J 8 position water heated cell changer 10 mm pathlength 1 80 2109 70 6 position Peltier heated cell changer 8
9. 80 2105 97 For pricing information visit www gelifesciences com orderonline Installation Qualification Operational Qualification IQ OQ NEw IQ OQ consists of an O OQ document and the related IQ OQ performance on site It covers e Inspection of goods supplied instrument PC and software e Basic operation of installed soft and hardware e SWIFT II SWIFT II CFR software qualification e Verification of Wavelength accuracy Wavelength repeatability Spectral bandwidth Absorbance accuracy Photometric reproducibility e Accessories qualification GE Healthcare ORDERING INFORMATION Product Quantity Code Number 1Q 0Q performance for Ultrospec NEW 1 28 9137 70 IQ OQ Documentation for Ultrospec NEW 1 80 2118 62 For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details IQ OQ Service can be provided for the following instruments e Ultrospec 2100pro e Ultrospec 3100pro e Ultrospec 5300pro e Ultrospec 6300pro For older instruments please contact your local GE Healthcare representative for details For more information visit www gelifesciences com f 1 wozoydop ds 1 NO 655
10. GE Healthcare office for details The cell holder supplied with Novaspec Plus accepts standard 10 mm pathlength glass or plastic cells adapters are available to convert it to accept 10 12 and 16 mm diameter test tubes It can be removed for cleaning or flushed through with water in situ if spillages occur For users who require temperature control particularly for kinetics studies and use with test kits a special version of NovaspecPlus is available with a factory installed heated cell holder 37 C only note that this cell holder must be factory installed and cannot be added after purchase Wavelength range 330 800 nm Bandwidth 7 nm Absorbance range 0 300 to 2 50 Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 fos 9 165 aA 435 nm Foon cat Output of kinetics to display Output of scan to display GE Healthcare Novaspec III Visible Soectrophotometer Novaspec Ill is robust compact reliable and versatile ideal for the student environment e Large display with easy to read characters Absorbance Transmission Concentration Factor and Rate Self test on startup Removable cell test tube holder for easy cleaning Analogue output for connection to chart recorder Grafico PC utility software including tutorial Novaspec Ill has been designed for use in the student teaching laboratory It is a rugged light in weight inst
11. NanoVue with integrated printer NEW 1 28 9232 15 NanoVue NEW 1 28 9232 16 NanoVue with wireless connectivity NEW 1 28 9232 17 Accessories Pathlength Calibration Kit 1 28 9244 05 Sample Plate Replacement kit 1 28 9244 06 For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details Wavelength range 190 1100nm Monochromator Flat grating Wavelength calibration Automatic upon switch on Spectral bandwidth 5nm Wavelength accuracy 0 02nm Wavelength reproducibility 0 1nm Light sources Pulsed xenon lamp Detector 1024 element CCD array 0 300 to 2 500A 0 to 199 T USB port standard Bluetooth option Photometric range Digital output Dimensions 260x390x100 mm Weight lt 4 5kg Power input 18Vdc from a 90 250V 50 60 Hz Max 30VA mains power pack Safety Certifications CE 89 336 EEC EMC directive CE 73 23 EEC LVdirective EN 61010 1 IEC1010 1 For new products and updated specifications please visit www gelifesciences com spectros Relevant parameters are calculated automatically and displayed at the touch of a button on a large display Up to 90 user defined methods can be stored with optional password protection Data output is via standard USB and PVC print via Computer software allowing data to be transferred to a PC or with the integrated printer or wireless Bluetooth options GE Healthcare
12. Power requirements External power adaptor 110 220 V or cell compartment area can be flushed with for example Approximate dimensions e a nn s formaldehyde or ethylene oxide to sterilize it Weight 0 6 kg The Ultrospec 10 uses a 600 nm LED source in combination with Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LV directive an advanced fiber optic to obtain ODg9g measurements which EN 61010 1 IEC1010 1 are comparable to those obtained on a regular spectrophotometer Measurements can be sent via a serial lead to a printer or can be downloaded to an appropriate program on a personal computer for analysis Rechargeable batteries provide nearly one month of cord free use For more information visit www gelifesciences com GE Healthcare Cells and Cuvettes for Spectrophotometry Cell Density Meter System Control Software UV Visible Cells ORDERING INFORMATION Product Quqntitu Code Number UV grade silica Standard rectangular cell with lid 1 mm pathlength 10 mm i d 200 ul working volume 12 5 x 45 mm external dimensions 1 80 2002 54 Standard rectangular cell with lid 5 mm pathlength 10 mm i d 1 ml working volume 12 5 x 45 mm external dimensions 1 80 2002 57 Standard rectangular cell with lid 10 mm pathlength 10 mm i d 2 ml working volume 12 5 x 45 mm external dimensions 1 80 2002 58 set of 2 matched 80 2099 89 set of 8 matched 80 2109 80
13. Spectrophotometry 2007 Dr Matthew Stremlau Why Old World monkeys are resistant to HIV 1 Premature disassembly of the HIV 1 capsid is caused by the rhesus monkey protein TRIM5a and a single amino acid change in human TRIM5a confers similar anti HIV 1 activity Dr Stremlau s grand prize winning essay describes how using q genetic screen TRIM5a was identified as the primary block to HIV 1 replication in Old World monkey cells Dr Stremlau received his BS in chemistry from Haverford College After graduation he spent 1 year as a Henry Luce Fellow at the National Laboratory for Agrobiotechnology in Beijing China before beginning graduate studies at Harvard University Here Dr Stremlau investigated retroviral restriction in nonhuman primates in Dr Joe Sodroski s laboratory While at Harvard Dr Stremlau cofounded the International Science and Health Network an equipment recycling program that sends scientific equipment to laboratories in the developing world He currently works in the U S Global AIDS Coordinator s Office at the State Department as an American Association for the Advancement of Science Fellow Dr Stremlau plans to start a postdoctoral fellowship in 2008 and is interested in emerging biotechnologies relevant to the developing world GE amp Science Prize for Young Life Scientists is supported by GE Healthcare and the journal Science which is published by AAAS the nonprofit society Used with permission o
14. acility for quantitation of Cy3 Cy5 fluorescein and user definable labeled cDNA probes for microarray studies these are particularly suitable for use with our CyScribe Labeling kits With its extensive range of built in software direct download to Microsoft Excel a wide range of accessories including a Sipper option and GLP self test diagnostics Ultrospec 6300 pro is a reliable compact and integrated system It can also be connected to a PC for complete control by SWIFT II Applications Software if required For conversion data for nucleic acids and proteins see the Technical Appendix GE Healthcare Supported by ORDERING INFORMATION Product Quantity Code Number Ultrospec 6300 pro 1 80 2117 55 Gold 1 0 nm bandwidth Ultrospec 6300 pro Classic 1 80 2117 60 1 0 nm bandwidth Ultrospec 6300 pro Yellow 1 80 2117 61 1 0 nm bandwidth Ultrospec 6300 pro Plum 1 80 2117 62 1 0 nm bandwidth Ultrospec 6300 pro Apple 1 0 nm bandwidth Includes Qualification and Performance Verification Logbook Spreadsheet interface software and serial interface cable Automatic wavelength calibration on startup GLP self diagnostics integral high resolution liquid crystal display with graphical user interface integrated software with post run calculations 8 position cell changer Available in a choice of colors 1 80 2117 63 For pricing information visit www gelifesciences com orderonline Relat
15. age recall of up to nine user defined methods are available The instrument startup menu can be customized so that the requirements of students and laboratory staff are met As experimental or measurement needs change the menu can be altered accordingly Ultrospec 500 pro has a 25 pin multipurpose output as standard converting to serial or analog for output to PC or chart recorder respectively with the appropriate interface In addition it can be used with standard parallel printers Ultrospec 500 pro can also be used in conjunction with SWIFT 1000 Applications Software GE Healthcare Supported by ORDERING INFORMATION Product Quantity Code Number Ultrospec 500 pro Classic 1 80 2112 50 Ultrospec 500 pro Yellow 1 80 2112 51 Ultrospec 500 pro Plum 1 80 2112 52 Ultrospec 500 pro Apple 1 80 2112 53 Accessories SWIFT 1000 Applications Software includes interface 1 80 2110 00 lead 2 position manual cell changer 10 mm pathlength 80 2109 04 Water heated cell holder 10 40 mm pathlength 80 2109 06 circulation bath required Peltier heated cell holder 25 30 37 C Temperature 1 80 2109 07 Controller required Temperature Controller 80 2109 01 Test tube holder accommodates diameters of 8 25 mm 80 2109 33 and heights of up to 180 mm Fitting kit for external sample delivery peristaltic pump 1 80 2109 08 and 10 mm flow cell required Spare Single Cell Holder U500 U1100 pro 1 80 2109 09 Pr
16. array hybridization probes and PCR o SL products e Accommodates 50 user stored methods making it suitable for the busy multi user or Quality Control laboratory SING Ealing e Provides display and print out information in English Results German French Italian Spanish or Russian Cy3 Absorbance Ratio 230 0 105 2607230 232 260 Odd 260 260 1 34 260 D127 3507260 0 375 Ultrospec 3100 prois the ideal UV visible spectrophotometer for the demanding laboratory where bench space is at q premium 430 o 001 Its many features include stored protocols for protein k r moet ee r cO ngiu pmolful Nucleotides Oye determination Bradford Biuret Lowry and BCA and nucleic 60 8 608 ns 12 15 0 605 62 0 acid quantitation characterization DNA RNA oligonucleotide 23 15 32 cen 5 220 xe theoretical Tm calculation The Ultrospec 3100 pro has built in Screen shot of all calculated results Screen shot of Cy3 labeled cDNA application software designed to measure and calculate the and useful ratios probe scan showing raw scan data incorporation of Cy3 Cy5 fluorescein or other user specified and calculated values AszaW0 OYdOI DEdS dyes into cDNA probes This is essential information for optimizing the concentration of each labeled probe before use in microarray hybridizations 12 UV visible scans of each probe are displayed on the high resolution display so that the integrity of the n
17. at made2measure ge com and Maintenance to keep your instrument running in peak condition visit us on the web at www gelivesciences com custom Additional services include equipment de installation To ensure that we handle your inquiry efficiently please provide re installation and software hardware upgrades Visit the product name catalog code and volume requirements www gelifesciences com labcrew for more information ON S A uo p pup1s 01 U0D Dd Ssi p ou duups ajdi3jnw 40 04 U09 Dqd JO JUaWINI SU jo1 u02 1n1pi dui 1 p u nok og uo p pup1s p Jajaid NOA p no A ON S A S5upi duio5 pi odooSpuiipud p u nok oq WSIUDYDaW pp 1 pup doig s 11 Ano S8 8AND JO wsiupyo w pp 1 pup doip D asn 0 UDM NOK og salpny s awAzua UOI1DO3IJI duup pup Suol1bollddp ajdizjny pup ul 1oid UOIPDIIIUNd plop DajONN luo in1 n2 jad pij 15pg uo Bulysom nok aav s uolipol ddp 1pu A uy tuuo1oudo4152 ds 3GIND NOI12313S GE Healthcare 638 UV Visible Spectrophotometers Pharmacopoiea Compliant Spectrophotometers Ultrospec 6300 pro UV Visible Spectrophotometer Stand alone fully Pharmacopoeia compliant instrument with a high resolution graphics display and press to read lamps Shown in Gold color option e Meets the demanding requirements of the Pharmacopoeia in terms of instrument specification and GLP self diagnostics with the benefits of being able to be used for rapid discrete measurement
18. ble 10 80 3000 77 lt 800 semi micro quartz 10 80 2002 77 gt 70 microvolume quartz 10 80 2103 69 gt 70 microvolume disposable 10 80 3000 81 10 250 gt 7 ultra microvolume quartzt 5 80 2103 68 100 2500 gt 3 Capillary cell for GeneQuant 100 or 0 5 80 2120 19 GeneQuant 1300 spare capillaries 100 80 2104 67 Assuming dsDNA with A269 1 0 for 50 ug ml ng ul in a 10 mm pathlength cell T This is supplied with a micro sample viewer accessory f Note that dilution may not be required for a miniprep PCR amplification where typical concentrations are between 50 and 200 ng ul GeneQuant 100 Spectrophotometer NEW ORDERING INFORMATION Product Quantity Code Number a GeneQuant 100 Classic NEW 1 28 9182 04 gt GeneQuqnt 100 Classic with Printer NEW 1 28 9182 05 For pricing information visit www gelifesciences com orderonline Availability varies bu region please contact your local GE Healthcare office for detai tails rd elqlls TECHNICAL Cc w Wavelength range 190 900nm Bandwidth 5nm O Safety Certifications CE 89 336 EEC EMC directive efed CE 73 23 EEC LVdirective O EN 61010 1 IEC1010 1 O For new products and updated specifications please visit O www gelifesciences com spectros Se e ofl SELECTION GUIDE Cells for use with GeneQuant 100 U Sample aD concentration O range Available Y after dilution sample Pathlength Code ng ul volume ul Sugg
19. ctrophotometer Product Quantity Code Number Ultrospec 2100 pro Classic 1 80 2112 21 Ultrospec 2100 pro Yellow 1 80 2112 22 Ultrospec 2100 pro Plum 1 80 2112 27 Ultrospec 2100 pro Apple 1 80 2112 28 Includes Automatic wavelength calibration on start up 8 position cell changer serial and parallel Centronics interfaces Available in a choice of colors For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details Related Products Refer To SWIFT II Applications Software page 655 UV Visible Cells page 651 Accessories for Spectrophotometers page 652 Offers high performance and versatility at an attractive price Shown in Classic TECHNICAL SPECIFICATIONS I e Robust compact and reliable for general laboratory Wavelength range 190 900 nm measurements Bandwidth Lanm e Xenon lamp for longer lifetime by incorporating press to Absorbance range 3 000 to 3 000 read technology and lower running costs three year lamp Safety certifications CE89 336 EEC EMCdirective warranty CE73 23 EEC LVdirective EN 61010 1 IEC1010 1 e Provides stored routines for DNA RNA and oligonucleotide quantitation and purity check e Combines basic measurement modes with enhanced software functionality and method storage e Can be upgraded with SWIFT II Applications Software to form q powerful system e Incl
20. d Cell Accessories ORDERING INFORMATION Product Quantity Code Number Cyclindrical Cell Cylindrical Cell 100 mm pathlength 22 mm diameter UV grade silica requires 80 2106 10 cylindrical cell holder 1 80 2003 12 Test tubes Glass test tubes pack of 10 marked for optical alignment 12 x 100 mm 1 80 2004 50 D Continuous Flow through Cells Continuous Flow through Cell 10 mm pathlength 3 mm internal diameter 75 ul volume 12 5 x 45 mm external dimensions UV grade silica 1 80 2003 05 D Continuous Flow through Cell 10 mm pathlength 4 mm internal diameter 450 ul volume 12 5 x 45 mm external dimensions optical glass 1 80 2004 45 Sipper Flowcell Sipper Flowcell 10 mm pathlength 80 ul internal volume UV grade silica includes tubing kit 1 80 2080 60 O Cell Spacers Cell Spacers for use with cells that have an 8 5 mm optical center pack of 6 1 80 2106 85 Packing Pieces L 1 mm pathlength cell packing pieces 1 80 2107 70 Q 5 mm pathlength cell packing pieces 1 80 2107 71 O For pricing information visit www gelifesciences com orderonline O 4 Cell Holders Y ORDERING INFORMATION Product Quantity Code Number Single Cell Holders 1 2 Cell holder up to 10 mm pathlength 1 80 2106 05 Cell holder for use with magnetic flea 10 mm pathlength requires magnetic stirrer 1 80 2108 10 Heated Single Cell Holders Water heated cell holder 10 40 mm pathlength requires circulation bath 1 80 2106 0
21. d measure sample In rate mode a kinetics assay can be followed at two wavelengths simultaneously for the first time on this level of product the student can follow in real time both the increase in absorbance as product is formed with the concomitant decrease as reactant is used up The cell holder supplied with Novaspec Ill accepts standard 10 mm pathlength glass or plastic cells adapters are available to convert it to accept 10 12 and 16 mm diameter test tubes The cell holder can be removed for cleaning or flushed through with water in situ Wavelength range 330 800 nm Bandwidth 7 nm Absorbance range 0 300 to 2 50 Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 For more information visit www gelifesciences com AsjaW0JOYdOI DEdS 12 649 Spectrophotometry 12 650 Visible Spectrophotometers Cell Density Meter Supported by ORDERING INFORMATION Ultrospec 10 Cell Densitu Meter Product Quantity Code Number Ultrospec 10 Cell Densitu Meter Classic 1 80 2116 30 Ultrospec 10 Cell Densitu Meter Yellow 1 80 2116 31 Ultrospec 10 Cell Densitu Meter Plum 1 80 2116 32 Ultrospec 10 Cell Densitu Meter Apple 1 80 2116 33 Accessories S2000P serial printer includes serial cable 1 80 3000 94 Spreadsheet interface software requires serial interface 1 80 2110 73 cable Disposable Cells 10 mm pathlen
22. ddition the benefits of enhanced software functionality including standard curve mode reaction rate slope calculation multiwavelength equation definition and method storage recall of up to nine user defined methods are available The instrument start up menu can be customized so that the requirements of students and laboratory staff are met As experimental or measurement needs change the menu can be altered accordingly x 3HEH A 0 391 0 8 300 A 500 Output of scan to display GE Healthcare Variable Wavelength Spectrophotometers Supported by ORDERING INFORMATION Product Quantity Code Number Ultrospec 1100pro Classic 1 80 2112 00 Ultrospec 1100pro Yellow 1 80 2112 01 Ultrospec 1100pro Plum 1 80 2112 02 Ultrospec 1100pro Apple 1 80 2112 03 Accessories SWIFT 1000 Applications Software includes interface 1 80 2110 00 lead 2 position manual cell changer 10 mm pathlength 1 80 2109 04 Water heated cell holder 10 40 mm pathlength 1 80 2109 06 circulation bath required Peltier heated cell holder 25 30 37 C Temperature 1 80 2109 07 Controller required Temperature Controller 1 80 2109 01 Test tube holder accommodates diameters of 8 25 mm 1 80 2109 33 and heights of up to 180 mm Fitting kit for external sample delivery peristaltic pump 1 80 2109 08 and 10 mm flow cell required Spare Single Cell Holder U500 U1100 pro 1 80
23. e Print preview of results with format customization e Excellent export to spreadsheet capabilities SWIFT II Applications Software provides application modules for wavelength scanning reaction kinetics quantitation multi wavelength time drive fraction analysis T and high throughput bacterial cell culture work These comprehensive software modules are proven and excellent for people who wish to export results to Microsoft Excel essential for consistent report preparation ORDERING INFORMATION Product Quantity Code Number SWIFT II METHOD 1 80 2108 31 Includes wavelength scanning reaction kinetics time drive quantitation multi wavelength fraction analysis cell culture and Tm No interface lead included SWIFT II SPECIALIST 1 80 2111 91 Includes multi wavelength time drive fraction analysis cell culture and T No interface lead included SWIFT II LAB 1 80 2108 26 Includes wavelength scanning reaction kinetics quantitation No interface lead included For pricing information visit www gelifesciences com orderonline The top of the range package SWIFT II METHOD comprises all of the modules on q CD ROM and is supplied as standard with the Ultrospec 5300 pro UV Visible Spectrophotometer The entry level package SWIFT II LAB caters for the fundamental applications of scanning kinetics and quantitation The less frequently used applications of multiwavelength time drive fraction analysis ce
24. ed Products Refer To 21 CFR part 11 compliant SWIFT II Applications Software page 654 SWIFT II Applications Software page 655 UV Visible Cells page 651 Accessories for Spectrophotometers page 652 Amersham CyScribe First Strand cDNA Labeling Kit page 338 Amersham CyScribe Post Labeling Kit page 339 Amersham CyDye Fluorescent Nucleotides page 342 Wavelength range 190 1100 nm in 0 1 nm data steps Bandwidth 1 0nm Absorbance range 3 000 to 3 000 Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 As well as fulfilling the European Pharmacopoeia supplement 2000 resolution test product 0 8 specific Ultrospec 6300 0 6 pro can export results 0 4 i directly to Microsoft 0 2 Ro Holmium perchlorate direct to PC 1 2 14 Absorbance Excel i Co tt Oo OA O nn AO O A DO DH no Wo YD Oo Oo 0 350 381 412 443 474 722 753 784 Wavelength s z 1 Absorbance Ratio 230 0 105 2607230 232 2607280 1 34 3507260 0375 260 edd 260 127 430 0 001 330 o 0g2 cDNA Yield Total cCONA 60 5 608 na Concentration Dyef Probe cONA ng ul pmolfnl MucleotidessDye 12 15 0 605 62 0 EER 15 32 cel 5 270 xe Press lt rur on Sample Hs 15 31 cel 5 220 Xe Screen shot of Cy3 labeled cDNA probe scan showing raw scan data and calculated values Screen shot of all calculated results and useful ratios
25. ested cell type mm number 5 125 gt 2000 standard quartz 10 80 2002 58 1 2 e Predefined methods for nucleic acid purification protein gt 800 semi microdisposable 10 80 3000 77 assays and cell culture density measurements lt 800 semi micro quartz 10 80 2002 77 e Large easy to read graphical display gt 70 microvolume quartz 10 80 2103 69 e Optional built in printer or output data via USB to a PC for gt 70 microvolume 10 80 3000 81 printing or storage disposable e Optics with no moving parts for high energy throughput 10 250 gt 7 ultra microvolume 5 80 2103 68 combined with a Xenon source for long lamp life time quartzt e Three year lamp warranty 100 2500 gt 3 capillary 0 5 80 2120 19 spare capillaries 100 80 2104 67 Applications Assuming dsDNA with A so 1 0 for 50 ug ml ng ul ina 10 mm pathlength Pre stored methods for DNA RNA and oligonucleotide purity cell and concentration in ug ml ng ul ug ul pmol ul and pmol t This is supplied with a micro sample viewer accessory measurements Visualization of nucleic acid scan allows t Note that dilution may not be required for a miniprep PCR amplification where impurities to be detected particularly useful for RNA samples typical concentrations are between 50 and 200 ng ul Proteins can be quantitated by BCA Biuret Bradford Direct UV and Lowry The OD of bacterial cell culture solutions can be Sample size measured to optimize harvest times Samples as low as 3ul can be analysed using
26. f AAAS 2007 Further information on how to enter plus past winners and their essays can be found at www gelifesciences com science Chapter 12 UV Visible Spectrophotometers Pharmacopoiea Compliant Spectrophotometers 639 Variable Wavelength Spectrophotometers 641 NEW NanoVue Spectrophotometer 644 Variable Wavelength Spectrophotometers Molecular Biology 645 NEW GeneQuant 100 Spectrophotometer 646 Visible Spectrophotometers Variable Wavelength Spectrophotometers 647 Cell Density Meter 650 Cells and Cuvettes for Spectrophotometry 651 Accessories for Spectrophotometers 653 Fluorometry 654 Software 655 System Control Software 655 Data Exportation Software 655 NEW Installation Qualification Operational Qualification IQ 0Q 655 made2measure Products in this chapter can be customized to your precise requirements including small scale packs concentrations special blends specific QC testing and scale up In addition we offer a Room Temperature Stable reagent development and manufacturing service where we develop your 20 C reagents and kits for storage and shipping at room temperature Our manufacturing standards are certified to ISO 9001 2000 with Six Sigma implementation throughout the manufacturing process Supported by All instruments in this chapter are Crew supported by Labcrew your GE Healthcare equipment professionals We provide Service Agreements and Preventive Contact us for more information
27. gth minimum volume 1 80 2004 53 2 5 ml Methacrylate pack of 100 Disposable Cells 10 mm pathlength minimum volume 1 80 2084 11 1 5 ml Polystyrene pack of 100 Adapter set for 10 and 12 mm tubes 1 80 3000 57 For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for Ultrospec 10 is suitable for cell density OD measurements details fn ce nh alla TECHNICAL SPECICATION measurement instrument Wavelength 600 nm e Battery operated for use where cells are actually cultured a 7 Bandwidth 40 nm e Easy to use easy to clean easy to sterilize Sas Ps e Download results to a PC or printer for convenient data p a storage and retrieval Accuracy lt 0 05 A at 1 A using neutral density filters i Repeatability 0 02 Aat1A The Ultrospec 10 Cell Density Meter measures the density of Cuvette holder Fixed with drain hole cells in suspension at 600 nm Small portable and easy to use Accepts 10 mm pathlength semi micro the instrument can measure growth rates of E coli other a I 14 17 mm round tubes bacteria and yeast it is not recommended for use with Output RS232 mammalian cell lines The hand held device can be easily I a I I ee Memory 99 readings employed in incubation cabinets under anaerobic conditions I Display Custom LCD and in other areas in the laboratory where cells are cultured ili b I f h f h
28. in any 21 CFR part 11 compliant environment it is incumbent on the end user to have the necessary standard operating procedures SOPs and training in place to ensure optimal use of the equipment SWIFT 1000 Applications Software SWIFT 1000 Applications Software provides application modules for wavelength scanning reaction kinetics and quantitation for the Ultrospec 500 pro and Ultrospec 1100 pro instruments Personal computer specification For optimum performance an IBM compatible 486 or greater personal computer running Microsoft Windows 95 98 NT or 2000 is required The PC should have a minimum of 8 Mb RAM 200 Mb hard disk a CD drive a serial mouse installed one free COMMS serial port and VGA graphics Any printer supported by Microsoft Windows 95 98 NT or 2000 can be used Contact GE Healthcare for further information For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number 21 CFR part 11 compliant SWIFT II software 1 80 2117 57 Includes wavelength scanning reaction kinetics time drive quantitation multi wavelength fraction analysis culture and T No serial interface cable included RS232C Serial Interface Cable 1 80 2105 97 For pricing information visit www gelifesciences com orderonline Personal Computer Specification 21 CFR part 11 compliant SWIFT II software requires a PC or network with one of the following Microsoft Windows operating
29. inter stand 1 80 2109 96 Tungsten Halogen Lamp Vertical Filament 1 80 2022 94 Deuterium Lamp Assembly Includes W lamp U1100 pro 1 80 2109 11 Only Interface for chart recorder 1 80 2109 03 Spreadsheet interface software 1 80 2109 02 Parallel printer cable 1 80 2071 87 Spare User Manual multi language 1 80 2112 04 Dust cover 1 80 2109 13 For pricing information visit www gelifesciences com orderonline Related Products Refer To SWIFT 1000 Applications Software page 654 Visible Cells page 652 Visible Cells page 652 Wavelength range 325 900 nm Bandwidth 5 nm Absorbance range 0 300 to 3 000 CE 89 336 EEC EMC directive CE 73 23 EEC LVdirective EN 61010 1 IEC1010 1 Safety certifications J36H A 0 4591 Zoom Output of scan to display For more information visit www gelifesciences com f 1 wozoydop ds 1 NO 647 Spectrophotometry 12 648 Visible Spectrophotometers Variable Wavelength Spectrophotometers Novaspec Plus Visible Spectrophotometer Novaspec Plus is an easy to use highly reliable and low maintenance instrument Flash Scan diode array Stored protein methods Bacterial cell culture measurement at OD oo Kinetics for activity studies Stored methods Grafico PC utility software Novaspec Plus is suitable for general biotech laboratory use It includes stored methods for Bradford BCA Biuret and Lowry protein quantitation in addi
30. length calibration on start up 8 position cell changer performance check integral high resolution liquid crystal display with graphical user interface integrated software with post run calculations Available in a choice of colors For pricing information visit www gelifesciences com orderonline Availability varies by region please contact your local GE Healthcare office for details Incorporates a high resolution liquid crystal display and built in CDNA application Related Products Refer To software Shown in Yellow color option SWIFT II Applications Software page 655 Displ hi P ase d UV Visible Cells page 651 j IPP gus Jap ane vA an Intuitive an easy to use Amersham CyScribe First Strand cDNA Labeling Kit page 338 interface on a high resolution liquid crystal screen Amersham CuSenbePostlabeling Kt page 339 e Xenon lamp for longer lifetime by incorporating press to Amersham CyScribe Direct mRNA Labeling Kit page 340 read technology and lower running costs three year lamp Pee TECHNICAL SPECIFICATIONS e Includes built in applications software for wavelength scanning enzyme kinetics standard curve substrate Wavelength range 190 900 nm concentration and multi wavelength equation entry Bandwidth lt 3nm e Includes cDNA application software for measuring Absorbance range 3 000 to 3 000 incorporation of Cy3 Cy5 fluorescein and user specified Safety certifications CE ee Eee dyes into purified micro
31. ll culture and Tm have been combined into a single package SWIFT II SPECIALIST Personal Computer Specification For optimum performance an IBM compatible 486 or greater personal computer running Microsoft Windows 95 98 NT 2000 or XP is required The PC should have a minimum of 8 Mb RAM 200 Mb hard disk a CD drive a serial mouse installed one free COMMS serial port and VGA graphics Any printer supported by Microsoft Windows 95 98 NT 2000 or XP can be used Contact GE Healthcare for further information Spreadsheet interface software e Provides the facility to export results directly to Microsoft Excel The Spreadsheet Interface Software provides the facility to export results directly to an Excel Spreadsheet For instance scan comprising data such as absorbance wavelength may be picked up as columns of numbers and converted to a more conventional graph using the spreadsheet Results may then be formatted or manipulated as appropriate prior to inclusion in reports or archiving ORDERING INFORMATION Product Quantity Code Number Spreadsheet interface software 1 80 2109 02 Compatible with Ultrospec 500 pro Ultrospec 100 pro and GeneQuant pro Includes serial interface cable Spreadsheet interface software requires serial interface cable Compatible with Ultrospec 2100 pro and Ultrospec 3100 pro Serial interface cable must be purchased separately RS232C Serial Interface Cable 1 80 2110 73 1
32. otein assays e Fast instrument startup with automatic self calibration e Provides Intuitive software for ease of use and large easy to read graphical display NanoVue spectrophotometer is an easy to use and reliable instrument for the measurement of nucleic acid and protein samples Samples of 0 5 ul to 2 ul can be pipetted directly onto a novel sample plate for measurement and then simply recovered using a pipette If sample recovery is not required the sample plate can be quickly and easily wiped clean NanoVue spectrophotometer provides a range of pre stored methods for the determination of RNA DNA and oligonucleotide concentrations and purity plus wavelength scanning to detect impurities Concentration measurements can be displayed in choice of units ug ml ng ul ug ul pmol ul and pmol Oligonucleotide primers can be characterized by keying in the base sequence up to 66 mer to obtain parameters such as conversion factor ug ml molecular weight theoretical absorbance AU umol and theoretical Tm Proteins can be quantitated by BCA Biuret Bradford Direct UV and Lowry with the ability to run up to 27 standards For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number NanoVue with integrated printer US and 1 28 9244 02 Canada only NEW NanoVue US and Canada only NEW 1 28 9244 03 NanoVue with wireless connectivity US 1 28 9244 04 and Canada only NEW
33. position water heated cell changer requires circulation bath 80 2106 04 80 2109 70 2 position manual cell changer 10 mm pathlength 1 80 2109 04 For pricing information visit www gelifesciences com orderonline Sipper Product Quantity Code Number Sipper complete with UV quartz cell and all tubing 1 80 2112 15 requires single cell holder 80 2106 05 or 80 2106 13 Marprene pump head tubes 6 for Sipper 1 80 2080 74 Viton pump head tubes 5 for Sipper 1 80 2106 99 PTFE flowcell tubing with connectors 1 80 2055 13 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Quantity Code Number Temperature Control Unit 1 80 2105 49 AsjawW0JOYUdOI DEdS For pricing information visit www gelifesciences com orderonline Printers ORDERING INFORMATION Product Quantity Code Number Printer Stand for Seiko DPU 414 thermal printer Ultrospec 1 80 2112 13 2100 pro 3100 pro 6300 pro only S2000P serial printer includes serial cable 1 80 3000 94 For pricing information visit www gelifesciences com orderonline GE Healthcare For more information visit www gelifesciences com 12 653 Spectrophotometry 12 654 Fluorometry Software System Control Software Accessories for Fluorometers ORDERING INFORMATION Product Quantity Code Number Hoechst 33258 Dye 100mg 80 6226 87 Calf Thymus DNA Fluorescence standard dried 250
34. re information visit www gelifesciences com GE Healthcare UV Visible Spectrophotometers Ultrospec 1100 pro UV Visible Spectrophotometer Ease of use and stylish design combined with good performance e Basic measurement modes coupled with enhanced software functionality and method storage Nucleic acid quantitation mode suitable for teaching laboratories Graphics for scan kinetics and standard curve Robust compact reliable and very easy to use Perfect for the biological science teaching and the industrial quality control laboratory Available in a choice of four exciting colors Ultrospec 1100 pro addresses the needs of both the biological science teaching and the industrial quality control laboratory The instrument has absorbance percentage transmission and factor concentration modes Absorbance against time plots and scans are shown on the graphics display there is also output directly to a spreadsheet or chart recorder Ultrospec 1100 pro has a 25 pin multipurpose output as standard converting to serial or analog for output to PC or chart recorder respectively with the appropriate interface In addition it can be used with standard parallel printers The instrument can also be used in conjunction with SWIFT 1000 Applications Software The UV capability of the Ultrospec 1100 pro is important for nucleic acid quantitation and purity check experiments which is now a vital part of many academic courses In a
35. rument with no moving parts making it both highly portable and highly reliable A fully automatic calibration at startup gives confidence in its use Novaspec Ill has a large display so that small groups of students can work around the instrument and view the results It will only measure in the modes of interest to this category of user absorbance transmission concentration and rate yet with the supplied Grafico utility software each instrument has the capability to download to a PC all results and a full wavelength range scan the requisite serial lead is also included Grafico also includes a full tutorial on the technique of UV Visible Spectrophotometry A combination of Novaspec III and the three experimental protocols for students described in the user manual provides an excellent package for technical staff GE Healthcare Visible Soectrophotometers Variable Wavelength Spectrophotometers Supported by ORDERING INFORMATION Product Quantity Code Number Novaspec Ill 1 80 2118 00 Accessories and other items Test tube adapters 10 12 16 mm 1 80 2117 47 Chart recorder interface cable 1 80 3003 55 Lamp Assembly Tungsten 1 80 2115 33 For pricing information visit www gelifesciences com orderonline Related Products Refer To Visible Cells page 652 Other Spectrophotometer Cells and Cell Accessories page 652 The instrument is extremely easy to use just select wavelength set reference an
36. s e Bandwidth of 1 0 nm and excellent scanning capability e Press to read high performance deuterium and tungsten lamps for reduced cost of ownership e Built in applications software with capability for 50 stored methods e Includes cDNA application software for measuring the incorporation of Cy3 Cy5 fluorescein and user specified dyes into purified microarray hybridization probes and PCR products e Provides display and print out information in English German French Italian Spanish or Russian Ultrospec 6300 pro is intended for the busy multi user laboratory environment encountered in many pharmaceutical and bio pharmaceutical companies Here use of an instrument conforming to the Pharmacopoeia may be a requirement even if many measurements are discrete as opposed to being part of a method development program Therefore the instrument has q Qualification and Performance Verification Logbook supplied as standard Using this an ongoing record of instrument performance can be maintained for GLP purposes For users who do not require the high resolution of a 1 nm bandwidth instrument the Ultrospec 3300 pro with a 1 8 nm bandwidth is also available Ultrospec 6300 pro has built in applications software for wavelength scanning enzyme kinetics standard curve substrate concentration and multiwavelength equation entry software In addition it has stored protocols for nucleic acid quantitation protein determination and a f
37. systems to be installed NT 4 0 with Service Pack 6 2000 with Service Pack 2 or 3 XP with Service Pack 1 Additionally at least one NTFS New Technology Filing System formatted directory must be available to use the software note that FAT and HPFS are not acceptable Contact GE Healthcare for further information ORDERING INFORMATION Product Quantity Code Number SWIFT 1000 Applications Software includes interface 1 80 2110 00 lead Includes wavelength scanning reaction kinetics quantitation interface lead For use with Ultrospec 1100 pro and Ultrospec 500 pro only For pricing information visit www gelifesciences com orderonline GE Healthcare Software 1Q 0Q System Control Software Data Exportation Software SWIFT II Applications Software X Microsoft Excel cDNA Cy3 xls al File Edit View Insert Format Tools Data Window Help l x JOSH en y s eg o c ej z amp h Z OB 100 ie ara 0 B z u Za m 8 S S A B a a jJ cDNA labelled with Cy3 Absorbance 225 275 325 375 425 475 525 575 625 Wavelength z 244 0 281 nd FMh cy3 Jal mi Ready SSS tn ea OA fam Je Astan HU Lotus Organizer Andre fp Andrew Dadd cc Mail M SS Microsoft Excel cD ES Paint Shop Pro N d 03 46 Export of a wavelength scan to Microsoft Excel e Includes an audit trail data log facility in each module
38. th scanning reaction kinetics time drive quantitation multiwavelength fraction analysis cell culture and T experiments An 8 position sample changer is supplied as standard for simple and fast sample handling For users who do not require the high resolution of q 1 nm bandwidth instrument the Ultrospec 4300 pro with a 1 8 nm bandwidth is also available Ultrospec 5300 pro satisfies the high specifications for resolution noise stability wavelength accuracy and reproducibility that are necessary for detailed spectral scanning In conjunction with a wide range of accessories the instrument is suitable for many applications in the method development and Quality Control laboratories in industry and in general laboratories in research institutions For more information visit www gelifesciences com Supported by ORDERING INFORMATION Product Quantity Code Number Ultrospec 5300 pro Gold 1 80 2117 56 1 0 nm bandwidth Ultrospec 5300 pro Classic 1 80 2117 70 1 0 nm bandwidth Ultrospec 5300 pro Yellow 1 80 2117 71 1 0 nm bandwidth Ultrospec 5300 pro Plum 1 80 2117 72 1 0 nm bandwidth Ultrospec 5300 pro Apple 1 80 2117 73 1 0 nm bandwidth Includes 8 position sample changer GLP self diagnostics SWIFT II METHOD software Windows 95 serial interface cable Available in a choice of colors For pricing information visit www gelifesciences com orderonline Please contact your local GE Heal
39. thcare office for computer and printer specifications Related Products Refer To 21 CFR part 11 compliant SWIFT II Applications Software page 654 SWIFT II Applications Software page 655 UV Visible Cells page 651 Accessories for Spectrophotometers page 652 Wavelength range 190 1100 nm in 0 1 nm data steps Bandwidth 1 0nm Absorbance range 3 000 to 3 000 Safety certifications CE 89 336 EEC EMC directive CE73 23 EEC LVdirective EN 61010 1 IEC1010 1 For conversion data for nucleic acids and proteins see the Technical Appendix IE SWIFT Il Wavescan Data 0 042 Toluene in Hezane k File Methods Edt View Run PostRun Window Help P66 yaa Abs 6 459 yas Mit 170 6 o Wd tma For Help press F1 European Pharmacopoeia Supplement 2000 test for resolution This test states that in order to verify the resolution of the instrument record the spectrum of a 0 02 v v solution of toluene R in hexane R The minimum ratio of the absorbance at the maximum around 269 nm to that at the minimum around 266 nm is stated in the monograph GE Healthcare UV Visible Spectrophotometers Variable Wavelength Spectrophotometers Ultrospec 3100 pro UV Visible Spectrophotometer Product Quantity Code Number Ultrospec 3100 pro Classic 1 80 2112 31 Ultrospec 3100 pro Yellow 1 80 2112 32 Ultrospec 3100 pro Plum 1 80 2112 37 Ultrospec 3100 pro Apple 1 80 2112 38 Includes Automatic wave
40. tion to the basic modes of absorbance transmittance ODsoo and concentration The use of diode array technology permits rapid wavelength scans to be made and as there are no moving parts makes for a highly reliable and low maintenance instrument The large backlit screen displays graphical results for wavelength scans kinetic assays including slope calculation for rate activity studies and standard curves Up to 99 methods can be stored for instant recall The Novaspec Plus is delivered with Grafico a PC utility software package and the requisite serial lead providing you with the means to capture print and store data from the instrument with a time date stamp onto a PC so that a results log can be built up or data exported to Microsoft Excel For more information visit www gelifesciences com Supported by ORDERING INFORMATION Product Quantity Code Number Novaspec Plus 1 80 2117 50 Novaspec Plus with heated cell holder 1 80 2117 51 Includes Grafico PC utility software serial cable dust cover Accessories and other items Test tube adapters 10 12 16 mm 1 80 2117 47 Chart recorder interface cable 1 80 3003 55 Lamp Assembly Tungsten 1 80 2115 33 For pricing information visit www gelifesciences com orderonline Related Products Refer To Visible Cells page 652 Other Spectrophotometer Cells and Cell Accessories page 652 Availability varies by region please contact your local
41. ucleic acid and fluorophore components of each probe can be inspected Yield fluorophore content and efficiency of incorporation are automatically calculated and displayed when starting RNA concentration and dilution factors are entered The software is A Samole E particularly suitable for probes labeled with CyScribe fluorescent labeling kits Ultrospec 3100 pro printer stand and a Seiko DPU 414 thermal printer Shown in Classic color option An 8 position sample changer is supplied as standard and the instrument will print out to standard Centronics parallel printers and export results to a PC With its extensive range of built in E AAAA AAN software a wide range of accessories including a Sipper option 57 90 1 317 Ultrospec 3100 pro display Od e ET protein determination using Press lt rur gt on Sample b 20 10 cen 8 360 xe Bradford method and GLP self test diagnostics Ultrospec 3100 pro is a reliable compact and integrated system for use in any busy laboratory including those which require data storage to a PC Ultrospec 3100 pro can also be used in conjunction with SWIFT II Applications Software if required For conversion data for nucleic acids and proteins see the Technical Appendix GE Healthcare For more information visit www gelifesciences com 641 Spectrophotometry 12 642 UV Visible Spectrophotometers Variable Wavelength Spectrophotometers Ultrospec 2100 pro UV Visible Spe
42. udes serial and parallel outputs as standard Ultrospec 2100 pro UV Visible Soectrophotometer is a simple to use instrument with advanced performance In addition to measuring absorbance and concentration it provides stored routines for nucleic acid quantitation and also a standard curve routine for protein determination Wavelength scan with zoom absorbance changes with time reaction rate determinations and standard curves can be displayed and printed out User defined equations can be entered using the multi wavelength mode and up to 18 methods can be saved The instrument can be upgraded for more sophisticated applications as well as data storage with SWIFT II Applications Software and a PC With its large sample compartment wide range of accessories such as the sipper option for high throughput customers and GLP self testing diagnostics Ultrospec 2100 pro is a versatile and reliable instrument for use in any laboratory Ultrospec 2100 pro with portable PC running SWIFT II software mounted on PC support plinth Shown in plum color option For conversion data for nucleic acids and proteins see the Technical Appendix Absorbance Time Drive Sample 001 Cell 3 16 58 Sample 001 Cell 3 340nm 10 22 0 161 Wavelength 260nm me a Penanasas i E E E E PETERE 5 T 15 20 25 30 Ultrospec 2100 pro display absorbance measurement Ultrospec 2100 pro display simple kinetics experiment For mo
43. ug 80 6227 06 4 MU standard kit 4 methylumbelliferone 100 mg 80 6227 25 Performance Validation kit 1 80 6252 52 Glass fluorometry cuvette 1 80 6227 44 Capillary adapter kit 1 80 6227 63 Includes 10 50 and 100 ul capillary tubes 20 of each Capillary tubes 10 pl 100 80 6227 82 Capillary tubes 50 pl 100 80 6228 20 Capillary tubes 100 ul 100 80 6228 01 Capillary Cuvette adapter kit 1 80 6228 39 Includes 9 ul capillary tubes 250 per package Capillary tubes 9 ul glass 250 80 6228 58 For pricing information visit www gelifesciences com orderonline 21 CFR part 11 compliant SWIFT II Applications Software Full password protection electronic records and signatures compliant Full audit trail All the functionality of standard SWIFT II Method software Compatible with Ultrospec 2100 3100 3300 4300 5300 and 6300 pro 21 CFR part 11 SWIFT II Applications Software provides full FDA compliance in terms of electronic records and signatures with the flexibility and functionality of the established SWIFT II software suitable for any analytical or QC laboratory that operates within such an environment The software comprises both client and server applications for networked installations but both of these can be installed on one PC if required When combined with the fully Pharmacopoeia compliant Ultrospec 6300 pro and 5300 pro instruments extremely powerful and sophisticated systems are generated Note that as
44. ume UV grade silica 1 80 2076 38 Microvolume cell with black walls 10 mm pathlength 70 ul working volume 12 5 x 48 mm external dimensions UV grade silica 1 80 2103 69 Spare quartz capillaries 100 0 5 mm pathlength 3 ul working volume UV grade silica Cristaseal not included 1 80 2104 67 Spare Cristaseal 10 1 80 2109 79 Spare Micro Sample Viewer 1 80 2109 87 AN Disposable Cells O Disposable Cells 10 mm pathlength minimum volume 70 ul UV plastic ultra micro pack of 100 1 80 3000 81t OD Disposable Cells 10 mm pathlength minimum volume 800 ul UV plastic semi micro pack of 100 1 80 3000 77t O For pricing information visit www gelifesciences com orderonline O These cells are supplied with packing pieces to facilitate use in a standard 10 mm pathlength cell holder O t Availability varies by region please contact your local GE Healthcare office for details g z4 O D et t Microvolume cell with 1 NO Semi microcell with stopper and black walls 80 2002 81 Semi microcell with lid and black walls 80 2002 77 Microvolume cell with Ultra microvolume cell black walls including black walls with black walls 80 2103 micro sample viewer 80 20 76 38 68 80 2103 69 Capillary Cell Holder 80 2104 66 GE Healthcare For more information visit www gelifesciences com 651 Cells and Cuvettes for Spectrophotometry Accessories for Cell Density Meter System Control Software
45. wry assays with the ability to run up to 27 standards GeneQuant 1300 can be used to measure the OD of bacterial cell culture solutions to optimize harvest times Incorporation of Cy3 Cy5 fluorescein and user specified dyes into purified microarray hybridization probes or PCR products can be measured The kinetics mode allows a plot of absorbance against time to be supplemented with AA min hence you can calculate the correlation coefficient for the duration of an assay this can be converted directly to rate of reaction Relevant parameters are calculated automatically and displayed at the touch of a button on a large display Samples as low os 3ul can be analyzed using a capillary cell system A wide range of standard cuvettes may be used depending on the sensitivity or volume requirements The 15mm optical beam height makes the GeneQuant 1300 compatible with low volume quartz and disposable UV plastic cuvettes For more information visit www gelifesciences com f 1 wozoydop ds 12 645 UV Visible Spectrophotometers Visible Spectrophotometers Variable Wavelength Spectrophotometers Molecular Biology Variable GeneQuant 1300 Spectrophotometer continued SELECTION GUIDE Cells for use with GeneQuant 1300 Sample concentration range Available sample after dilution ng pl volume ul Suggested cell type Pathlength mm Code number 5 125 gt 2000 standard quartz 10 80 2002 58 gt 800 semi micro disposa
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