AZURA UVD 2.1S user manual
Contents
1. Analytical Flow Cells Technical data Path length 10 mm A4061XB Connection 1 16 Inner diameter 1 1 mm Volume 10 ul Material Stainless steel with heat exchanger Max flow rate 20 ml min Max pressure 300 bar UVD 2 1S User Manual V6820 Version 2 3 40 Accessories and Spare Parts Technical data Path length 10 mm A4061 Connection 1 16 A4074 fiber Inner diameter 1 1 mm optics version Volume 10 ul Material Stainless steel with heat exchanger Max flow rate 20 ml min Max pressure 300 bar Path length 3mm A4042 Connection 1 16 A4044 fiber Inner diameter 1 0 mm optics version Volume 2 ul Material Stainless steel Max flow rate 50 ml min Max pressure 300 bar Path length 3mm A4045 Connection 1 16 A4047 fiber Inner diameter 1 0 mm optics version Volume 2 ul Material PEEK Max flow rate 50 ml min Max pressure 30 bar Preparative Flow Cells Technical data Path length 0 5 1 25 2mm A4066 Connection 1 8 A4078 fiber Inner diameter 2 3 mm optics version Volume 1 7 4 3 6 8 ul Material Stainless steel Max flow rate 1000 ml min Max pressure 200 bar UVD 2 1S User Manual V6820 Version 2 3 Accessories and Spare Parts 41 Technical data Path length 0 5 1 25 2mm A4067 Connection 1 8 A4079 fiber Inner diameter 2 3 mm optics version Volume 1 7 4 3 6 8 ul Material PEEK Max flow rate 1000 ml min Max pressure 100 bar Path length 0 5 1 25 2mm A40682. Cap fittings for the fiber optics Note Do not touch the ends of the fiber optics with your fingers as this could falsify the measurement UVD 2 1S User Manual V6820 Version 2 3 36 Maintenance and Care 1 Manually unscrew the fittings of the fiber optics from the flow cell Fig 27 Fiber optics fittings on the flow cell Seal the fiber optic connectors with caps S e PSom Fig 28 Cap fittings on the fiber optics Manually unscrew the fiber optics from the detector A Fig 29 Fiber optic connectors on the detector UVD 2 1S User Manual V6820 Version 2 3 Technical Data 37 Technical Data Detection Detector type Small variable single wavelength UV detector Small variable single wavelength UV detector with fiber optics con nectors Light source Deuterium D gt lamp with inte grated GLP chip Wavelength range 190 500 nm Spectral bandwidth 13 nm at H line FWHM Wavelength accu 3nm racy Wavelength preci 0 7 nm ASTM E1657 98 sion 2 0 x 10 AU at 254 nm 2 5 x 10 AU at 254 nm fiber optics version ASTM E1657 98 3 0 x 104 AU h at 254 nm 4 0 x 107 AU h at 254 nm fiber optics version ASTM E1657 98 Linearity gt 2 0 AU at 270 nm ASTM E1657 98 Time constants 0 00 0 02 0 05 0 1 0 2 0 5 1 0 2 0 s UVD 2 1S User Manual V6820 Version 2 3 38 Accessories and Spare Parts Communication Maximum data rate 50 Hz LAN 20 Hz Analog
3. 10 Hz RS 232 Input Autozero Start IN Error either IN or OUT 0 10 V Analog IN Error either IN or OUT Analog output 1x 2 5 V scalable 20 bit Control Digital RS 232 LAN DHCP remote connector Analog wavelength control Manual front panel Technical Lamp operating hours parameters Ambient condi Temperature range 4 40 C 39 2 104 F tions Air humidity Below 90 non condensing General Power supply External Input 100 240 V output 24 V DC 60 W Dimensions 121 x 129 x 187 mm Width x Height x 121 x 129 x 236 with flow cell Depth Height above sea maximum 2000 meters level Accessories and Spare Parts You will find further information on spare parts and accessories online www knauer net Note For repeat orders of spare parts use the enclosed packing list Contact the Technical Support in case there are any questions on spare parts or accessories UVD 2 1S User Manual V6820 Version 2 3 Accessories and Spare Parts 39 Devices and Accessories AZURA UV Detector UVD 2 1S AZURA UV Detector UVD 2 1S without flowcell flow cell ADAOO AZURA UV Detector UVD 2 15S Geesse See Optics Version with Ge out flow cell German user manual V6820 English user manual A Accessories Accessories kit Fiber Optic Connectors Name Order umber Available Flow Cells Most flow cells are also available equipped with fiber optical connectors for the use with the fiber optics version of the detector
4. the path length is factory set to 2 mm 3 mm or 10 mm You can remove the spacers on one or both sides and hence change the path length to 1 25 mm or 0 5 mm Prerequisite Flow cell has been disassembled Tools Allen screwdriver size 3 UVD 2 1S User Manual V6820 Version 2 3 Initial Startup 19 Fig 7 Preparative flow cell Legend Spacer Threaded ring Seal holder compression bushing Cover Light guide with PTFE seal Procedure 1 Using the allen screwdriver unscrew and remove the threaded ring 2 Remove the cover and spacer Put the spacer aside 3 Insert the cover 4 Using the allen screwdriver tighten the threaded ring Enlarging the optical path length To enlarge the optical path length put in one or both spacers Connecting the Capillaries Capillaries connect the detector to other devices and lead liquids Prerequisite Flow cell has been assembled UVD 2 1S User Manual V6820 Version 2 3 20 Initial Startup Tools Torque wrench Caution Damage to the flow cell caused by strongly tightened fittings Check the torque of screw fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings Note PEEK fittings withstand a maximum pressure of 400 bar Process 1 Push the capillary through the fitting Push the clamping ring onto capillary Manually screw together the capillary and the flow cell To avoid leaks tighten the fitting
5. 24 Operation Control Choose between analog or digital ports to control the device accordingly LAN DHCP Control with LAN IP address is obtained automatically recom mended RS 232 9600 Serial port for cables longer than 2 m RS 232 115200 Serial port for cables with up to 2 m length ANALOG Control with applied voltage Prerequisite Where applicable a physical connection has been established Status display is active Keep left arrow key pressed Press right arrow key until the correct Control display appears LAN DHCP Let go of left arrow key Using both arrow keys choose a Fig 14 Display port port When setting is finished the status display appears Activating Deactivating the Lamp The status display shows not only absorption and wavelength but also indicates the status of the lamp HEA Deuterium lamp is heating OFF Deuterium lamp is deactivated ON Deuterium lamp is activated Prerequisite Status display is active Process Keep left arrow key pressed Press right arrow key until the correct LAMP Di display appears ORE Let go of left arrow key Using both arrow keys activate or deactivate the deuterium lamp Fig 15 Display Lamp UVD 2 1S User Manual V6820 Version 2 3 Operation 25 When setting is finished the status display appears Adjusting the Chromatogram To get a better representation of the chromatogram you can either smooth the
6. Connection 1 4 A4081 fiber Inner diameter 4 0 mm optics version Volume 1 7 4 3 6 8 ul Material Stainless steel Max flow rate 10000 ml min Max pressure 200 bar Path length 0 5 1 25 2mm A4068 2 Connection 1 4 Inner diameter 4 0 mm Volume 1 7 4 3 6 8 ul Material Stainless steel Max flow rate 10000 ml min Max pressure 200 bar Path length 0 5 mm A4069 Connection 1 16 A4089 fiber Inner diameter 0 8 mm optics version Volume 3 ul Material Stainless steel Max flow rate 250 ml min Max pressure 200 bar UVD 2 1S User Manual V6820 Version 2 3 42 Technical data Path length Connection Inner diameter Volume Material Max flow rate Max pressure Accessories and Spare Parts 0 5mm 1 16 0 8 mm 3 ul PEEK 250 ml min 100 bar Fiber Optics Preparative Flow Cells Technical data Path length Capillary connection Material Max pressure Path length Capillary connection Material Max pressure Path length Capillary connection Material Max pressure Path length Capillary connection Material Max pressure Path length Capillary connection Material Max pressure Path length Capillary connection Material Max pressure 10 mm 1 2 with TRI Clamp PEEK and fused silica 10 bar 7mm 3 8 with TRI Clamp PEEK and fused silica 150 bar 0 5 1 25 2 mm 1 2 with TRI Clamp Stainless steel and fused silica 80 bar 0 5 1 25 2 mm 3 4 with TRI Clamp Sta
7. Leaks 9 Lens Cleaning 32 Line voltage Power supply 12 Location 12 M Maintenance 29 UVD 2 1S User Manual V6820 Version 2 3 Maintenance Tasks 30 Modifiers 7 O Opening the device 10 Operating hours Deuterium lamp 26 Operation 21 Index 45 T Technical Support 12 test Installation Qualification 28 Operation Qualification 29 Test cell 6 17 Time constant 25 Arrow keys 22 U Autozero 22 UV light 10 Handling 22 W OQ 29 Wavelength 21 P Pin header 14 Port 24 Analog 24 LAN 24 Serial with 115200 baud rate 24 Serial with 9600 baud rate 24 Power supply 10 12 Power cable 10 Power strip 10 Professional group 8 R Repair kit for preparative flow cells 39 S Salts 7 Self calibration 21 Setup 22 Software Lokal Area Network 27 Serial port 28 Solvent tray 10 Solvents 7 Flushing solution 31 Spare Parts 8 Spring strip ANALOG IN port 15 Error IN OUT port 15 Start IN port 15 Start IN see spring strip 15 UVD 2 1S User Manual V6820 Version 2 3 lh KNALIER Declaration of Conformity Producer KNAUER Wissenschaftliche Gerate GmbH Hegauer Weg 38 14163 Berlin Deutschland Model Type Detector UVD 2 1S Product no EDA00 EDA05 Reference The product complies with the following standards Machinery Machinery Directive 2006 42 EC IEC 60799 1998 EMC EMC Directive 2004 108 EC IEC 61000 3 2 2009 IEC 61326 1 2006 Disposal ROHS Directive 2011 65 EU WEEE Directive 2012 19 EU Safety Low Volta
8. Manual V6820 Version 2 3 16 Unpacking and Setup Process Place the terminal strip on a suit able surface Push the depressor tool into the opening on the upper side Continue pushing the depressor tool down and lead the cable into the front end of the terminal O strip Remove the depressor tool Check whether the cables are j tightly attached B o Plug the terminal strip onto the af multi pin connector Fig 4 terminal strip Next steps Finish the installation and perform the initial startup Analog Control Using the analog port you can control the wavelength by changing the applied voltage The analog control must first be selected in the SETUP menu The con trol voltage must be applied against GROUND see Using the Analog Port on page 26 To use the analog port for controlling the detector you have to set a zero point and enter a scaling value Zero point at 0 V 000 nm Scaling 100 nm per Volt If 5 V voltage is applied the wavelength is 500 nm Ground Voltages which may influence the measuring result can be discharged if the device is connected to a grounded device e g a thermostat Tools Depressor tool Note When connected to a 24 V power supply the detector is not grounded UVD 2 1S User Manual V6820 Version 2 3 Initial Startup 17 Process Remove 5 mm of insulation from the ends of the cable Using the depressor tool o
9. User Manual V6820 Version 2 3 Unpacking and Setup 15 AUTOZERO A signal short circuit to GROUND sets the measuring signal to zero ERROR IN OUT IN After receiving a signal short circuit to GROUND from an external device an error message appears and the device stops OUT An error signal is active for as long as an error is displayed e g the lamp does not start GROUND Reference point for external analog control of the detec tor ANALOG IN Allows external analog control of the detector when the option ANALOG has been selected in the SETUP menu The control voltage must be applied against GROUND Start IN After receiving a signal short circuit to GROUND from an external device the device starts If controlled via software an electronic trigger is sent via LAN Pin Header for Remote Control To control one device through another you use the multi pin connector To use remote control you have to connect cables to the terminal strip everything comes included with delivery The single ports are used to exchange control signals Prerequisites The device has been turned off The power plug has been pulled Tools Depressor tool Caution Short circuit hazard Turn off the device before connecting it to the multi pin con nector Pull the power plug Caution Electrostatic discharge can destroy the electronics Wear a protective bracelet against electrostatic discharge and ground UVD 2 1S User
10. connected to a computer Status display is active Keep left arrow key pressed Press right arrow key until the correct Control display appears 9600 Let go of left arrow key Using the arrow key select the baud rate Fig 22 Display control When setting is finished the status display appears Functionality Tests IQ and OQ Installation Qualification IQ The customer may request the Installation Qualification which is free of charge In case of a request the Technical Support of KNAUER or from a provider autho rized by KNAUER performs this functionality test during the installation The Installation Qualification is a standardized document that comes as part of the delivery and includes the following confirmation of flawless condition at delivery check if the delivery is complete UVD 2 1S User Manual V6820 Version 2 3 Maintenance and Care 29 certification on the functionality of the device Operation Qualification OQ The Operation Qualification includes an extensive functionality test and must be purchased from the manufacturer Contact the KNAUER Sales Department to request an offer The Operation Qualification is a standardized KNAUER docu ment and includes the following definition of customer requirements and acceptance terms documentation on device specifications device functionality check at installation site Test Intervals To make sure that the device operates within the
11. output signal or adjust the voltage absorption ratio Smoothing output signal To smooth the output signal you have to change the values of the time con stant 0 00 s 0 02 s 0 05 s 0 1s5 0 2s 0 55 15 25 Prerequisite Status display is active Keep left arrow key pressed Press right arrow key until the correct TC display appears Cass Let go of left arrow key Using both arrow keys set a value Fig 16 Display time constant When setting is finished the status display appears Adjusting V AU ratio By assigning different absorption values to a voltage value of 1 V you can stretch or compress the chromatogram You can choose the absorption value out of 4 values 0 01 AU 0 1 AU 1 AU 10 AU Prerequisite Status display is active Keep left arrow key pressed Press right arrow key until the correct WOU NV display appears 0 OLAU Let go of left arrow key Using both arrow keys set a value ig 17 Display analog ut UVD 2 1S User Manual V6820 Version 2 3 26 Operation When setting is finished the status display appears Checking the Deuterium Lamp Note The deuterium lamp should be checked regularly According to Good Laboratory Practice GLP a deuterium lamp is to be in operation for no longer than 2000 operating hours Displaying Operating Hours Prerequisite Status display is active Keep left arrow key pressed Press y GLP Lamp right arrow key until t
12. Ih KNAUER feura Detector UVD 2 15S User Manual V6820 Note The contents of this user manual apply for the BlueSha dow product Please submit a request on any article numbers for BlueShadow Table of Contents 3 Table of Contents Note For your own safety be sure to read the manual and always observe the warnings and safety information on the device and in the manual Intended Uses ERENNERT eee a ee AEE Eee AE 5 PETEROM EE n A E E EEEE E E E e 5 EEE E E eee EE E E E E E A 6 E ala e e Ee AE aes H Scope of Delivery EE 8 Safety for Users 0 cee eee ee eee eee ees 8 Definition of Personal and Material Damages 10 Svmbols and Signs nn 11 Unpacking and Setup 12 Contacting the Technical Support 12 Location Requirements 12 Power s pply oerion e e eee eee a eee eee e 12 Space Requirements 0 0 0 0 0 ee ccc ee eee ee 13 Unpacking s se ee ee eG Roa Rod Ree EE E e 13 Connectors on the Rear Side 13 Control via the spring strip 2 0 ee eee ee eee eee 14 Slide le un EC 14 Pin Header for Remote Control 15 Analog Control sssri E aes Sg es eee 16 GrOUN eee eek ae doe eda ee bes eee ee 16 I itial Startup uer ee ae deel Tee EN 17 Installing the Flow Cell 0 0 2 ec eee eee eee eee ee 17 Setting the Optical Path Length of a Preparative Flow Cell 18 Connecting the Capillaries 0 eee eee ee ee 19 Switching the Detector On 20 EIDEN Ee NENNE
13. N ne ea Pee 21 Basic Operation at the Device 21 UVD 2 1S User Manual V6820 Version 2 3 4 Table of Contents Control of the Device 22 Menu Structure 23 UE Cl e 5 se chee esi sss eset aa Goda EE EE bE 24 Activating Deactivating the Lamp 24 Adjusting the Chromatogram 25 Smoothing Output sine ai EIER ER eb Ged henge whip lace E AA 25 Adjusting VAU tations eege EE Gebei tee Pad aes 25 Checking the Deuterium Lamp 26 Displaying Operating Hour 26 Using the Analog Port 26 Setting the Zero Point 26 Entering sa Sealing Values sie ccs oink geleed cash ile A gd ELE E 27 Control with Chromatography Software 27 Local Area Network LAN 27 Serial interface 28 Functionality Tests IQ and OO 28 Maintenance and Care 29 Maintenance Contract 2 eee ee eens 29 Cleaning and Caring for the Device 0 2 eee eee eee 30 What Maintenance Tasks can Users Perform on the Device 30 Cleaning the Flow Cell 0 0 eee ee ee ene 31 Rinsing the Flow Cell 31 Cleaning the Lens of an Analytical Flow Cell 32 Cleaning the Light Guide of a Preparative Flow Cell 33 Replacing the Flow Cell 34 Replacing the Fiber Optics 0 0 eee eee eee eee 35 Removing the Fiber Optics 0 eee eee eee eee 35 Technical Datas en 2 4 va joes gaia a ig Goa oF ah SE NENNEN 37 Accessories and Spare Parts 38 Devices and Accessories 39 Fiber Opt
14. ccur Symbols and Signs The following symbols and signs can be found on the device in the chromatog raphy software or in the user manual High voltage hazard Electric shock hazard Electrostatic discharge hazard damages to system device or components can occur General warning sign moderate injuries can occur and also damages to system device or components UV light hazard eye injuries can occur A device or system marked with CE fulfills the product specific requirements of European directives This is con firmed in a Declaration of Conformity UVD 2 1S User Manual V6820 Version 2 3 12 Unpacking and Setup Unpacking and Setup Contacting the Technical Support You have various options to contact the Technical Support Phone 49 30 809727 111 Fax 49 30 8015010 E mail support knauer net You can make your requests in English and German Location Requirements The location for the device must meet the following requirements level surface for device or system Protect from heavy ventilation Weight 1 5 kg Dimensions 121 x 129 x 187 mm Width x Height x Depth Power supply 24 V DC Humidity below 90 non condensing Temperature range 4 40 C 39 2 104 F Caution Defect of the device due to overheating possible Set up the device in such a way that it is protected against exposure to direct sunlight Keep at least 15 cm clear at the rear and 5 10 cm at each s
15. e g used at universities research institutions and routine lab oratories Features One of the smallest HPLC detectors on the market The deuterium lamp measures spectra between 190 nm to 500 nm A wide range of flow cells for analytical or preparative LC applications with flow rates from 10 l min up to 10 l min Automatic recognition and storage of device specific information which are important for Good Laboratory Practice Operation Qualifications OQ for repairing the device Automatic and current diagnosis of the device Control with chromatography software or with the front panel Easy integration of the detector into complex chromatography systems Options Various types of flow cells are available to the user Pay attention to the compa tability of the flow cells see Accessories and Spare Parts on page 38 Note A test cell is preassembled to the device Before the detector can be used for measurements the test cell has to be replaced by a flow cell The UVD 2 15S Fiber Optics Version is delivered without a test cell UVD 2 1S User Manual V6820 Version 2 3 Intended Use 7 Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected solvents was compiled based on research in the pertinent lit erature and is only a recommendation If there is any doubt contact the Techni cal Support of the ma
16. e safety relevant information is listed in alphabetical order in the following table Topic Explanations Decontamination Contamination of devices with toxic infectious or radio active substances poses a hazard to all persons during operation repair sale and disposal of a device All con taminated devices must be properly decontaminated All materials or fluids used for decontamination must be collected separately and disposed of properly Flammability Organic eluents are highly flammable Since capillaries can detach from their screw fittings and allow eluent to escape it is prohibited to have any open flames near the analytical system Regularly check if any system components are leaking UVD 2 1S User Manual V6820 Version 2 3 Safety for Users 10 Explanations Solvent tray Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray Eluent lines Install capillaries and hoses so as liquids cannot get into the interior in case of a leak Power strip If several devices are connected to one power strip always consider the maximum power consumption of each device Power cable Defective power cables are not to be used to connect the device to the mains power Self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions Power supply Only connect devices to voltage sources whose
17. ge Directive 2006 95 EC IEC 61010 1 2011 The mark of conformity has been applied to the rear panel Date Berlin 01 10 2014 Alexanda Knauer CEO and owner C The product was tested with a typical configuration Revision 02 Part of V6820 KNAUER Wissenschaftliche Ger te GmbH All rights reserved Technical data are subject to change without notice Please check our website for latest updates and changes Translation of the original German edition of this manu al version 2 3 Last manual update 2015 07 20 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of KNAUER Wissenschaftliche Gerate GmbH See up to date manuals online www knauer net downloads HPLC SMB Osmometry KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Telefax 49 308015010 E Mail Il L KNAUER info knauer net Internet www knauer net KNAUER 2015 V6820 0 0 04 14 Westkreuz
18. he correct S 1234 1h display appears Let go of left arrow key Fig 18 Display operating hours of lamp The number of operating hours is displayed and afterwards the status display appears Next steps If the deuterium lamp has been operating for approximately 2000 hours con sider lamp replacement Using the Analog Port To use the analog port for controlling the detector you have to set a zero point and enter a scaling value Setting the Zero Point The zero point is predefined by the applied voltage Prerequisite Device has been connected to GROUND ANALOG port has been selected Keep left arrow key pressed Press right arrow key until the correct A IN display appears set zero Let go of both arrow keys Press autozero Fig 19 Display Analog In set zero The applied voltage is set as the zero point and is adjusted to 0 nm wavelength UVD 2 1S User Manual V6820 Version 2 3 Operation 27 Entering a Scaling Value It is recommended to set 500 nm at an applied voltage of 5 V Prerequisite Device has been connected to GROUND ANALOG port has been selected Zero point has been set Process Apply voltage Keep left arrow key pressed Press A IN right arrow key until the correct Onm display appears Let go of left arrow key Fig 20 Display Analog In Scaling 4 Using the arrow keys set a value Do not press autozero When setting is finished the status display ap
19. ic Connectors 2 0 0 eee eee eee eee 39 Available Flow Cells vince noes eae ale ieee reer eens 39 Preparative Flow Cell 40 Fiber Optics Preparative Flow Cell 42 RE TEE EE EEEREEEEEEER 43 Disposals er a a 43 Iddee ioe nhac MERE A alae ai AE E EEE 44 Declaration of Conformity 2 0 ccc eee eee eens 46 UVD 2 1S User Manual V6820 Version 2 3 Intended Use 5 Intended Use Note Only use the device for applications that fall within the range of the intended use Otherwise the protective and safety equipment of the device could fail Device Overview The detector is a measuring device that is used for measuring UV spectra quickly and precisely Fig 1 Detector UVD 2 1S front view v i E ERROR INOUT AMALOGIN E STARTIN E ae eo ECH uya Made in Germany Bog Fig 2 Detector UVD 2 15S rear view UVD 2 1S User Manual V6820 Version 2 3 6 Intended Use Legend Integrator Output RS 232 port Connection LAN port Ground Pin header Fan Operating range The detector can be used in analytical and preparative HPLC system It is used in laboratories to analyze substance mixtures In a HPLC system the detector serves to detect substances in liquids and show their concentration The device can be used in the following areas Biochemistry analysis Chemical analysis Food analysis Pharmaceutical analysis Environmental analysis The detector is
20. id chromatography Knowledge regarding substances that are suitable only to a limited extent for use in column liquid chromatography Knowledge regarding the health risks of chemicals Participation during an installation of a device or a training by the company KNAUER or an authorized company UVD 2 1S User Manual V6820 Version 2 3 Safety for Users 9 If you do not belong to this or a comparable professional group you may not perform the work described in this user manual under any circumstances In this case please contact your superior Personal Safety Equipment for Professional Group When working with the device take measures according to lab regulations and wear protective clothing safety glasses with side protection protective gloves overall What must be taken into account All safety instructions in the user manual The environmental installation and connection specifications in the user manual Observe national and international regulations pertaining to laboratory work Original spare parts tools and eluents made or recommended by KNAUER Good Laboratory Practice GLP For the development of methods and validation of devices Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory Ameri can Journal of Medical Technology 44 1 pages 30 37 1978 Accident prevention regulations published by the accident insurance compa nies for laboratory work Mor
21. ide for air circulation Power supply The device is only suitable for 24 V direct current Only the supplied power unit is to be used to connect the device to the mains supply Replace defective power unit only with original accessories from KNAUER Only use power units with a permission for use from your country In case of queries contact the Technical Support Note Make sure that the power plug on the rear of the device is always accessible so that the device can be disconnected from the power supply UVD 2 1S User Manual V6820 Version 2 3 Unpacking and Setup 13 Space Requirements Side clearance to other devices At least 5 cm if there is another device on one side At least 10 cm if there are devices set up on both sides At least 15 cm on the rear panel for the fan Unpacking Store all packing materials Included packing list should be kept for repeat orders Tools Utility knife Caution Damage to the flow cell caused by carrying the detector incor rectly Lift the detector up with your hands grasping the side panels Procedure 1 Check for damages caused during transportation In case you notice any damage contact the Technical Support and the forwarder company 2 Set up the delivery so the label is in the correct position Using the utility knife cut the adhesive tape Open the delivery 3 Remove the foam inserts Take out the accessories kit and the manual 4 Open the accessories kit and ta
22. inless steel and fused silica 80 bar 0 5 1 25 2 mm 3 8 with TRI Clamp Stainless steel and fused silica 150 bar 0 5 1 25 2 mm 1 4 with TRI Clamp Stainless steel and fused silica 80 bar UVD 2 1S User Manual V6820 Version 2 3 A4095 A4096 fiber optics version Disposal 43 Test Cells Technical data Test cell normal A4123 A4125 fiber optics version holmium oxide filter A4126 A41 28 fiber optics version filter stray light A4146 A4148 fiber optics version Disposal Hand in old devices or disassembled old components at a certified waste facility where they will be disposed of properly AVV Marking in Germany According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactured by KNAUER are marked as waste electrical and elec tronic equipment 160214 WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German Elektroaltger teregister EAR The number belongs to category 8 and 9 which among others comprise laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufac tured by KNAUER back to the distributor the importer or the company free of charge but would be charged for the disposal Solvents and Other Operating Materials All solvents and other operating materials must be collected
23. ke out all accessories Check the scope of delivery In case any parts are missing contact the Technical Support 5 Grip the device at its side panels near the middle and lift it out of the pack aging 6 Remove the foam inserts from the device 7 Check for damages caused during transportation In case you notice any damage contact the Technical Support 8 Set up the device in its appropriate location 9 Remove the protective foil and transportation securing device standard detector only Connectors on the Rear Side All connectors are located on the rear side of the detector UVD 2 1S User Manual V6820 Version 2 3 14 Unpacking and Setup INTEGRATOR S i J Gw Fig 3 Detector UVD 215 rear view Legend Integrator Output RS 232 port Connection LAN port Ground Spring strip Fan External devices like computers fraction collectors etc can be connected in 3 different ways to the detector Connected via the spring strip remote connector Connected via LAN within a network Connected via RS 232 Control via the spring strip Caution Electrostatic discharge can destroy the electronics Wear a protective bracelet against electrostatic discharge and ground The spring strip is used for remote control The single ports transport start con trol and error signals Spring Strip Assignments Cowan a o o GROUND Reference point of the voltage at the signal inputs UVD 2 1S
24. n turned off or is heating the absorption values are substituted by OFF or HEAT respec tively There are 3 buttons on the detector that can be used for basic operation Using the buttons you can monitor the device and change the settings UVD 2 1S User Manual V6820 Version 2 3 22 Operation Note To avoid falsifying measuring values KNAUER recommends to press the Autozero button before every measurement and after exchanging eluents Keep left arrow key pressed Scroll using the right lt gt arrow key FE E Press any of the arrow keys to set values and to Fig 11 Arrow keys change settings O Initiating an autozero Absorption is set to 0 000 WS Fig 12Autozero Control of the Device You can control the device using the buttons on the front panel Handle the buttons in the following manner Handling 1 Keep left arrow key pressed Scroll through the menu using the right arrow key 2 Let go of left arrow key and using both arrow keys set the values UVD 2 1S User Manual V6820 Version 2 3 Operation Menu Structure UVD Start up routine Ce Status display Lamp status LAMP D2 ON Time constant Integration time Light intensity Control Analog Out Analog In zero point Analog In scaling Serial number i Fir re Firmware Boer as Abb 13 Menu structure 23 LAMP D2 LAMP D2 EIN HEA Control ANALOG UVD 2 1S User Manual V6820 Version 2 3
25. nufacturer Not suitable eluents Halogenated hydrocarbons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing particles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid Acetone at 4 25 C 39 2 77 0 F Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane at 4 25 C 39 2 77 0 F lsopropanol Carbon dioxide liquid 99 999 CO gt Methanol a valid for the specified temperature range UVD 2 1S User Manual V6820 Version 2 3 8 Scope of Delivery Suitable eluents Phosphate buffer solutions 0 5 M Toluol Dilute ammonia solution Dilute acetic acid 10 50 at 25 C 77 0 F Dilute sodium hydroxide 1M Water Scope of Delivery Note Only use original parts and accessories made by KNAUER or a company authorized by KNAUER Accessory Kit AZURA User Manual EN DE Installation Qualification Documents EN DE Transport securing device standard detector only a Test cell not supplied with Fiber Optics Version Safety for Users Professional group The user manual is addressed to persons that are qualified as chemical labora tory technicians or have completed comparable vocational training The following knowledge is required Basic knowledge of liqu
26. pears Control with Chromatography Software The detector can be controlled with e g OpenLAB EZChrom edition version A 04 05 or higher ChromGate version 3 3 2 or higher and ClarityChrom ver sion 3 0 7 or higher You will find a detailed description on the chromatography software in the soft ware manual There are two advantages to the use of software Continuous operation for triggered measurement is possible System status can be checked in the software To be able to control the detector using chromatography software the com puter must be connected to the device either with a RS 232 cable or a LAN cable Local Area Network LAN A detector connected to a LAN is recognized by the software and automatically receives an IP address because it is set to Dynamic Host Configuration Protocol DHCP at the factory Prerequisite Device has been connected to LAN Status display is active UVD 2 1S User Manual V6820 Version 2 3 28 Functionality Tests IQ and OQ Process Keep left arrow key pressed Press right arrow key until the correct Control display appears AN DHCP Let go of left arrow key Using the arrow keys set to LAN DHCP Fig 21 Display control When setting is finished the status display appears Serial interface The user can choose between two ports 115200 Serial port for cables with up to 2 m length 9600 Serial port for cables longer than 2 m Prerequisite Detector is
27. pen the port Ground on the spring strip Ne Insert the first cable end Connect the second cable end to the port Ground on the spring strip of a grounded 220 V KNAUER device The physical connection is established Initial Startup Note Before the detector is ready for use a flow cell must be installed Before installing the flow cell the optical path length in the flow cell can be changed The optimal path length depends on the type and the quantity of the sample Installing the Flow Cell The test cell has no connectors for capillaries and must therefore be replaced by a flow cell before first usage Prerequisite Detector has been switched off Power plug has been pulled UVD 2 1S User Manual V6820 Version 2 3 18 Initial Startup Note The test cell is used during operation qualifications and must be stored Unscrew the 2 knurled head screws Pull out the slide Remove the test cell 4 Insert the flow cell o into the slide 5 Push the slide into the detector Tighten the knurled head screws Fig 6 Assembling the flow cell Flow cell is assembled The next step is connecting the capillaries Setting the Optical Path Length of a Preparative Flow Cell WARNING Irritation of retina through UV light Concentrated UV light can leak out from the flow cell or the fiber optic connectors Switch off the device and pull the power plug Depending on type
28. renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or with water in an ultra sonic bath 5 Afterwards assemble the flow cell and make sure that the new seal ring does not interrupt the light path 6 Using the allen screwdriver tighten the threaded ring Result Flow cell can be assembled What to do when If the cleaning of the lens does not have the desired effect the lens has to be replaced Cleaning the Light Guide of a Preparative Flow Cell The preparative flow cells have a rod shaped light guide instead of the concave lens of the analytical cells Fig 24 Preparative flow cell UVD 2 1S User Manual V6820 Version 2 3 34 Maintenance and Care Legend Spacer Threaded ring o Compression bushing Cover Light guide with seal ring Prerequisite Device has been switched off Power plug has been pulled Flow cell has been removed Tools Tweezers Allen screwdriver size 3 Procedure 1 Using the screwdriver loosen the threaded ring 2 Remove the cover and the spacer not part of all flow cells 3 Using the tweezers pull out the compression bushing and the light guide OF 4 Carefully push the light guide out of the holder and strip off the seal The seal ring needs to be renewed during every cleaning of the fiber optics 5 Clean the light guide with a clean soft cloth or with an appropriate solvent in an ultrasonic ba
29. separately and dis posed of properly All wetted components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed first with isopropanol and then with water before being maintained disassembled or disposed UVD 2 1S User Manual V6820 Version 2 3 44 Index A Absorption 21 25 Accessories 8 oo for the Fiber Optics Detector Index Additives 7 ANALOG IN see spring strip 15 Analog port 16 Example 16 Scaling 27 Zero point 26 AUTOZERO 15 Autozero Button 22 AVV marking 43 C Capillary Clamping ring 20 Fitting 20 Torque 20 Care 29 Checking on the Deuterium Lamp 26 Chromatogram 25 Axis scale 25 Output signal 25 Cleaning the Device 30 Cleaning the light guide 33 Connectors 13 Contact 12 Control 24 D declaration of conformity 46 Decontamination 9 Definition of Personal and Material Damages 10 Detector Basic operation 21 Front view 5 Rear view 5 14 Deuterium lamp Maximum operating hours 26 Status 24 Device test Operation Qualification OQ 29 Display Menu structure 23 Status display 21 E Eluent 7 Eluent lines 10 Eluents 7 Flammability 9 Self ignition point 10 Toxicity 10 Error IN OUT see spring strip 15 F Flow Cell Cleaning 31 Flow cell 6 Analytical 39 Disassembly 34 Installation 17 Optical path length 17 18 Rinsing 31 Types 39 G Ground 16 GROUND see spring strip 14 l Integrator see analog port 16 IQ 28 L
30. ses with side protection protective gloves and an over all All wetter components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed with isopropanol first and water afterwards before being maintained disassembled or disposed WARNING Irritation of retina through UV light Concentrated UV light can leak out from the flow cell or the fiber optic connectors Switch off the device and pull the power plug Caution Performing maintenance tasks on a switched on device can cause damage to the device Switch off the device and pull the power plug Users may perform the following maintenance tasks themselves Regularly check the operating hours of the deuterium lamp According to Good Laboratory Practice GLP a deuterium lamp is to be in operation for no longer than 2000 operating hours Inspect the flow cell assembly Clean the flow cell Replace the flow cell Replace the fiber optic connectors Fiber Optics Version only Order numbers of the required spare parts can be found in the chapter Accesso ries and Spare Parts UVD 2 1S User Manual V6820 Version 2 3 Maintenance and Care 31 Cleaning the Flow Cell Increased baseline noise and reduced sensitivity can be a result of a dirty flow cell Often it is sufficient to rinse the flow cell to restore optimal sensitivity Note Dirty lenses or fiber optic connectors could falsify the measure ment Do not to
31. specified range you should test the device using the Operation Qualification at following intervals Every 3 months average useful life of more than 5 days week or 24 hours day when operating with buffer solutions or other salt solutions Every 6 months average useful life of 1 to 5 days week Execution The test can be carried out either by the Technical Support of KNAUER or from a provider authorized by KNAUER Maintenance and Care Proper maintenance of your HPLC device will ensure successful analysis and reproducible results Maintenance Contract The following maintenance work on the device may only be performed by the manufacturer or a company authorized by the manufacturer and is covered by a separate maintenance contract Opening the device Removing the hood or the side panels UVD 2 1S User Manual V6820 Version 2 3 30 Maintenance and Care Cleaning and Caring for the Device Caution Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol What Maintenance Tasks can Users Perform on the Device Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated When performing maintenance tasks on the device always wear safety glas
32. th Make sure that the clean light guide is not touched by fingers 6 Afterwards assemble the flow cell and make sure that the new seal ring does not interrupt the light path 7 Using the screwdriver tighten the threaded ring Result Flow cell can be assembled What to do when If the cleaning does not have the desired effect the light guide has to be replaced Replacing the Flow Cell Prerequisite Detector has been switched off Power plug has been pulled Capillaries are disconnected UVD 2 1S User Manual V6820 Version 2 3 Maintenance and Care 35 Process 1 Unscrew the 2 knurled head screws Pull out the slide Remove the flow cell Insert the flow cell into the slide Push the slide into the detector Tighten the knurled head screws Fig 26Assembling the flow cell Flow cell is assembled The next step is connecting the capillaries Replacing the Fiber Optics UV light will cause the fiber optics to become blind with time solarization making them no longer suitable for use Observe the following regarding the use of fiber optics Do not touch the ends of the fiber optics with your fingers as this could fal sify the measurement Handle the fiber optics with care avoid impacts or hard actions Move the fiber optics carefully without using pressure or bending it Removing the Fiber Optics Prerequisites The device has been switched off Tools
33. uch the lens or the fiber optic connector lends with bare hands Wear gloves Rinsing the Flow Cell The following solvents are recommended for flushing HCl NaOH Ethanol Acetone Tools Syringe Caution Contamination of the flow cell caused by oil drops Do not use compressed air for drying Procedure 1 Fill the syringe with flushing solution 2 Inject it into the inlet of the flow cell and allow it to act for 5 minutes 3 Fill the syringe with water and inject again 4 Remove the flow cell from the detector and use a nitrogen stream to dry it Result Flow cell is clean Next steps Check if the baseline noise has disappeared If the flushing does not have the desired effect all flow cells can be disassem bled to clean the lens UVD 2 1S User Manual V6820 Version 2 3 32 Maintenance and Care Cleaning the Lens of an Analytical Flow Cell Fig 23 Analytical flow cell Legend Compression part Seal ring o Threaded ring Lens Prerequisite Device has been switched off Power plug has been pulled Flow cell has been removed Tools Tweezers Allen screwdriver size 3 Procedure 1 Using the allen screwdriver loosen the threaded ring 2 Using tweezers or by gently tapping on a clean surface remove the com pression part UVD 2 1S User Manual V6820 Version 2 3 Maintenance and Care 33 3 The lens is protected by a seal ring This must be
34. voltage equals the device s voltage Toxicity Organic solvents are toxic above a certain concentra tion Ensure that work areas are always well ventilated Wear protective gloves and safety glasses when working with the device UV light Concentrated UV light can disperse from a detector without a flow cell or from the fiber optic connectors which leads to irritation of the retina When the flow cell or the fiber optic connectors are replaced switch the device off and pull the power plug Where is use of the device prohibited Never use the system in potentially explosive atmospheres without appropriate protective equipment For further information contact the Technical Support of KNAUER see Contacting the Technical Support on page 12 Take the device out of operation At any time take the device completely out of operation by either switching off the power key or by pulling the power plug Opening the device The device may only be opened by the Technical Support of KNAUER see Contacting the Technical Support on page 12 Definition of Personal and Material Damages Possible dangers related to the device may be divided into personal and mate rial damages in this user manual UVD 2 1S User Manual V6820 Version 2 3 Symbols and Signs 11 Category Explanations DANGER Lethal or very serious injuries can occur WARNING Serious injuries can occur CAUTION Moderate injuries can occur and device defect can o
35. with a maximum torque of 5 Nm for stainless steel fittings or 0 5 Nm for PEEK fittings by using the open end wrench Fig 9 Capillary and flow cell Result Capillaries have been connected and the detector is ready for operation Switching the Detector On Caution Possible damage to the device caused by condensed water Allow device to acclimate for 3 h before it is connected to power supply and taken into operation UVD 2 1S User Manual V6820 Version 2 3 Operation 21 Prerequisite Installation has been completed Flow cell has been assembled Procedure Switch off the power supply 1 2 Plug the power supply into the device 3 Connect the power supply and the plug 4 Plug in the power supply 5 Using the power supply switch switch on the detector Result The detector starts its self calibration The display shows the progress that has been made When the self calibration has finished the status display appears If the self calibration fails switch the detector off and back on Practical Tipp Regularly check the capillaries for leaks Operation The detector can be operated in two ways Control via the buttons on the front panel Control with chromatography software Basic Operation at the Device The status display shows the values for absorption and wavelength MOROA ER Fig 10 Status display Legend Absorption Wavelength Note When the deuterium lamp has bee
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