Human EGFR (Total) - Thermo Fisher Scientific
Contents
1. invitrogen Human EGFR Total Singleplex Bead Kit Technical data Sheet Catalog LHR9061 Lot A10263 Note A letter at the end of the lot number signifies an additional packaging of this same lot Intended Use This kit is comprised of components for the measurement of EGFR in cell lysates Buffer reagents needed to complete the reaction are sold separately under Catalog LHB0002 These reagents are intended for use in the Luminex 100 or 200 System only Note This kit uses a Co Incubation Assay format See the User Manual included in the Intracellular Protein Buffer Reagent Kit Cat LHB0002 for further information Before attempting to multiplex this kit with any other kits please contact Technical Support Reagents Provided Human EGFR 1 Part LM197 Description Antibody Bead Concentrate 10X Lot A10236 Size 0 25 mL 100 tests Bead Region 68 Form 0 25 mL 10x bead concentrate solution in storage buffer Contains 0 05 sodium azide as a preservative Storage Light sensitive material Store at 2 8 C in the dark until the expiration date indicated on the kit Human EGFR Total 2 Part DN197 Description Detection Ab Concentrate 10x Lot A10235 Size 0 50 mL 100 tests Form 1 0 mL of a 10x stock of Detection Antibody Concentrate Contains 0 1 sodium azide as preservative Concentration of antibody is matched to this lot of beads Do not mix lots of Coated Beads and Detection Antibody Storage Store at2. 2 8 C until the expiration date indicated on the kit 3 Part SM057 Description RTK Total 5 plex Standard Lot S012505 Size 2 Vials Form This standard lyophilized recombinant proteins is designated in ng mL Contains 0 1 sodium azide as preservative The proteins in this standard have been calibrated with their respective Invitrogen ELISA kits Detailed information on calibration is provided below Storage Store at 2 8 C Use within 1 hour after reconstitution The remaining reconstituted standard should be discarded or frozen in aliquots at 80 C for further use The standard can be frozen and thawed one time without loss of immunoreactivity Concentration of Reconstituted Standards IR 15 ng mL c Kit 0 175 ng mL c Met 17 5 ng mL EGFR 15 ng mL IGF 1R 1 25 ng mL Important note The concentrations of reconstituted standards are lot specific Please verify all concentration values entered in data analysis software Reconstitution Reconstitute in 2 0 mL Assay Diluent Recommended Starting Concentration for Standard Curve Upon reconstitution the starting concentration of standards is the value cited above Make 2 fold serial dilutions in Assay Diluent Refer to the User Manual included in the kit for further information Calibration All markers in this reagent set were calibrated to their respective Invitrogen ELISA kits catalog numbers IR KHR9111 IGF 1R KHOO0491 EGFR KHR9061 c Kit KHOO391 and c M
3. et KHOO0251 Subsequent lots of standard will be normalized to this lot of material to allow consistency of quantitation Species Reactivity This reagent set recognizes human proteins Other species have not been tested For research use only CAUTION Not for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 e Tel 800 955 6288 E mail techsupport invitrogen com PILHR9061 Rev 06 10 Page 1 of 2 DCC 10 1613 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use Human EGFR Total Singleplex Bead Kit Technical data Sheet Performance Characteristics Typical Standard Curve EGER Total Protein EGER Total Intra assay variation n 16 Inter assay variation n 32 Analytical Sensitivity The analytical sensitivity was determined by adding two standard deviations to the median fluorescence intensity obtained when the zero standard was assayed 32 times Recovery To evaluate recovery analytes were spiked at 3 different concentrations into 10 Cell Extraction Buffer Cat FNNOO11 and 10 NP40 Cell L
4. r European Community authorised representative Protect from light fl Consult accompanying documents Directs the user to consult instructions for use IFU accompanying the product By purchasing this Kit which contains fluorescently labeled microsphere beads authorized by Luminex Corporation Luminex you the customer acquire the right under Luminex s patent rights to use this Kit or any portion of this Kit including without limitation the microsphere beads contained herein only with Luminex s laser based fluorescent analytical test instrumentation marketed under the name Luminex 100 or 200 This product is covered by one or more of the following U S patents 6 046 807 For research use only CAUTION Not for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 e Tel 800 955 6288 E mail techsupport invitrogen com PILHR9061 Rev 06 10 Page 2 of 2 DCC 10 1613 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use
5. ysis Buffer Cat FNN0021 Note This is a representative standard curve Absolute MFI values will vary Sample Preparation This kit has been validated with cell lysates prepared in Cell Extraction Buffer Cat FNNOO11 and NP40 Cell Lysis Buffer Cat FNN0021 Optimization of cell stimulation and cell lysis procedures may be required for each specific application Refer to User Manual included in the Buffer Reagent Kit for detailed instructions EGFR Total Parallelism Example Data To evaluate the performance of this kit natural EGFR from A431 cell lysate was serially diluted in Assay Diluent The median fluorescence intensity of each dilution was plotted against the EGFR standard curve Parallelism is presented in the figure to the right and indicates that the standard accurately reflects EGFR content in samples MFI Effect of Sample Treatment Buffer on EGFR Signal in Cell Lysate 5 ug mL The data presented to the right demonstrate the impact of the Sample Treatment Buffer pre incubation step on the observed signal In this study A431 cells were lysed in Cell Extraction Buffer Lysates were either treated with Sample Treatment Buffer STB or the Sample Treatment Buffer incubation step was omitted STB Explanation of symbols Description Symbol Description Batch code Research Use Only In vitro diagnostic medical device Use by Temperature limitation Manufacture
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