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FastGene GelPic LED Box Manual

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1. 5 2922 NIPPON Genetics SuSE EUROPE GmbH FestGene FastGene GelPic LED Box 2013 April Rev 01 FastGene GelPic LED Box CONTENTS GENERAL 2 dlaigee licc 2 PACKAGING CONTENT tritt ttti dab nets kin dra rette reo tere HE BERAN WERE EV N 3 SETUP OF THE INSTRUMENT ky c k disasik kerekek bakl aaa 4 Rien wciclkme 5 SCANNING WESTERN BLOT COLORIMETRIC STAINED esee 6 BROWSE SCANNED IMAGES DELETE SCANNED IMAGES 7 INSTALLATION OF AMBER FILTER 0 8 ACCESS STORED SCANNED IMAGE FILES WITH COMPUTER 9 DISPLAY SGANS ON Hi 9 FUNCTIONAL MENU FUNCTION DESCRIPTION l eese 10 FUNCTIONAL MENU FUNCTION DESCRIPTION ll eee 14 TECHNICAL SPECIFICATIONS sse eterne kk kK k kK kk A AA 18 ORDERING KAK KA KAK KAKA KAR kO 19 CONTACT INFORMATION AA K H ku Ek d k RAB u U AR 19 GENERAL INFORMATION Thank you for purchasing our FastGene GelPic LED Box This Manual includes the operation procedure In order to use the instrument properly please read this manual carefully before using FastGene GelPic LED Box This instrument is suitable for research use only FIRST QC CHECK Please check the instrument an
2. image at a time 2 X 2 Display 4 images at a time 3 X 3 Display 9 images at a time Press OK button to save the setting The captured images will display on the screen based on the selected preview format setting iT Up Mode Same as the Set Up Mode described in Function Descriptions I on page 13 FastGene GelPic LED Box TECHNICAL SPECIFICATION Image Capture Sensor Color CMOS Sensor Resolution 9M pixels Exposure Time 1 6 sex 11 scales Image Types JPEG Image Storage SD Card Storage View Area 16 x 11 5 cm fixed lens Lens and Filter Filter Wheel Manual Driven 2 position Emission Filter 520 nm Amber Filter Lightning Blue Light 470nm Blue LED Light Epi White Light White Back Light LED White Light LED White Light Panel Interface Display Panel Build in 2 7 Touch TFT LCD Image Storage SD Card Storage Output USB 2 0 Card Reader General Information TV out 3 5 mm end and RCA end Power Dimension W x H x D Gel Tools Weight Certification AC Adaptor 12V 1 5A 18W 198 x 227 x 254 mm Sample Tray Shovel Amber Shield 3 2 kg CE FCC FastGene GelPic LED Box ORDERING INFORMATION Product Cat No FastGene GelPic LED Box GP03 LED Related Products FastGene GelPic Imaging System LED GP01 LED FastGene GelPic Imaging System UV GP01 UV FastGene LED Transilluminator FG 06 FastGene UV Transilluminator FG 300
3. of the screen The captured images can then be reviewed one by one by pressing right arrow button Press OK twice to get back to Scan Mode It will take few seconds to complete the process Auto Play 6 When in Play Mode zi press OK to get into functional option list Press right arrow button to choose Auto Play Mode then press OK The captured image will automatically display on LCD screen one by one FastGene GelPic LED Box 4 Edit Mode When in Play Mode press OK to get into functional option list Press right arrow button to choose Edit Mode Press down arrow button get into Edit Menu Rotate Delete and select the desired setting item Press right arrow button to get into setting mode Rotate Right 90 Delete No Left 90 Yes 180 Flip H Flip V Press up and down arrow button to select the desired setting Press OK to save the setting Repeat previous step for delete mode setting or press OK again to return to Play Mode FastGene GelPicLED Box E Preview Mode Whenin Play Mode press OK to get into functional option list s EES Press right arrow button to choose Preview Mode Ela Press down arrow button to get into Preview Menu 1x1 2x2 3x3 and select the desired setting item Preview Mode 1 X1 Display 1
4. FastGene LED Illuminator FG 05 Mupid One Electrophoresis System Mu2 Mupid One LED Illuminator Mu4 Midori Green DNA Stain 1 ml MG02 Midori Green Advance DNA Stain 1 ml MG04 Midori Green Direct DNA Stain 1 ml MG06 Agarose Gel Band Cutter FG 830 FastGene Gel PCR Extraction Kit 100 FG 91202 FastGene Gel PCR Extraction Kit 300 FG 91302 CONTACT INFORMATION Nippon Genetics Europe GmbH Binsfelder Str 77 D 52351 D ren Phone 49 0 2421 2084690 Email info nippongenetics eu info nippongenetics de For more detailed product information contact details questions or trouble shooting please visit our English website www nippongenetics eu or our German website www nippongenetics de FastGene is a registered trademark of Nippon Genetics Europe GmbH FastGene GelPicLED Box 20
5. c board to avoid the background light for fluorescent stained gel scanning For colorimetric gel scanning leave the white backlight unblock on the lid FastBene GelPic LED Box SCANNING WESTERN BLOT COLORIMETRIC STAINED GEL In Scanning Mode lift scanner lid and take the sample tray out of the scanner 2 Place the blot membrane at the center of the tray for which the signal side of the membrane is facing toward the bottom of the tray 3 Place the sample tray back into the Lighting area of the scanner Slide the black plastic board into the groove of the white backlight to block the white light on the lid It is suggested to slide the black plastic board to block the white backlight on the lid for fluorescent stained gel scanning For colorimetric gel scanning leave the white backlight unblocked on the lid Close the scanner lid Slide the Light Selection Switch on the front side of the scanner to select the white light source for stained gel scanning 7 Set Transmitted or Epi item through Functional Menu Please check LED Lighting of Functional Descriptions section for details of turn on of Transmitted or Epi application Transmitted mode is to scan the gel stained with colorimetric stain such as the SDS PAGE gel stained with coomassie blue stain 8 Adjust the desired image quality through the Functional Menu if necessary Please check Functional Descriptions section for the detai
6. d the packing list when you first open the instrument packing case If you find there is something wrong with the instrument or the packaging content please contact Nippon Genetics Europe or your local distributor FastGene GelPic LED Box PACKAGING CONTENT FastGene GelPic LED Box Scanner Unit Black plastic board in box A C adapter 12V 1 5A DC Amber filter board in box USB 2 0 cable Gel shovel TV out cable Gel cutting knife SD card 4G Quick Installation Guide Sample tray in box Manual Front Figure 1 pper li Card reader Standalone LCD display Up Down Left Right menu button SD SDHC card slot Blue Light White Light selection switch Back Figure 2 Lighting area DC power USB 2 0 port TV Out port FastGene GelPic LED Box SETUP OF THE INSTRUMENT 1 2 3 4 Figure 5 Remove all tapes used to hold items in place for shipping Place scanner unit on flat surface Insert SD SDHC card up to 32GB Connect power source see Figure 2 Connect AC adapter to back of unit then plug into electrical outlet The Menu Mode icon will be displayed on the top left side of the display Figure 4 Wet the gel and place gel at the center of the sample tray Figure 5 Place sample tray into the Lighting area of the system Figure 6 Caution Do not place the gel directly on the surface of the Lighting area Fail to do so may lead LED damage caused by buffer lea
7. e amber filter board with the hole for hanging on lid in front Fig 8 4 Place the amber filter board onto the filter board hanging tab so the filter board may hang as shown in Figure 9 5 When hanging on the lid make sure the two small rods on the filter board are placed closely against the lid 6 Theinstallation is completed One may turn on blue LEDs for further gel cutting purpose m Filter board hanging tab Amber Filter Board Figure 8 Small rod Figure 9 FastBene GelPic LED Box ACCESS STORED SCANNED IMAGE FILES WITH COMPUTER The files stored on the SD SDHC card can be accessed by a computer 1 Connect the provided USB cable to the computer USB port 2 Connect the other end of the cable to the USB port on the back of the scanner Switch Card reader Standalone to Card Reader mode Through the operating system on PC the contents of the SD SDHC card will be available as removable media 5 Copy the files to PC s hard drive for storage or for further use DISPLAY SCANS ON TV The display from the scanner can be displayed on a television rather than the built in scanner preview screen using the AV video port PLO ey gt Set the television to AV mode see TV documentation for details Connect the included TV Out cable to the video port on the TV Connect the other end of the cable to the TV port on the back of the scanner Video output from the scanner can now be seen on the connect
8. ed TV including the full menu interface FastGene GelPic LED Box FUNCTIONAL MENU FUNCTION DESCRIPTION I LED Lighting Mode To select the white light application for ale Exposure Mode To adjust the exposure time frame in this mode B W Switch Mode To scan display the image in colorimetric or Set Up Mode To set up the following parameters Reset Yes No Reset all current settings back to factory default Scan Mode The object is able to be scanned in this mode Transmitted Epi image scan respectively Note this mode is available only when white light application is selected monochrome mode The default setting of the image is monochrome black amp white a LCD Dark Normal Bright b Display NTSC PAL NTSC USA PAL Europe 6 Quality S Fine Fine Normal JPEG Compression d Size 12M 9M 5M 1M Megapixels 9M is default setting e Power Save 1 min 3 min 5 min 10 min off Power shut off f Language English E88 rp 3c g Format Yes No Format SD card h FastBene GelPic LED Box E Mode When Ei icon displays on top left corner of the screen the object is ready to be scanned X Lighting Mode This mode is only available when switching to white light application Whenin Scan Mode press OK to get into functional option list Press right arrow button to choose LED Lighting Mode Press up or down arrow b
9. kage Start to adjust the image condition and scan Figure 4 Menu mode icon Sample tray Lighting area FastGene GelPic LED Box SCANNING GEL In Scanning Mode lift scanner lid and take the sample tray out of the scanner Wet the gel and place the gel at the center of the sample tray Place the sample tray back to the Lighting Area of the scanner Caution Do not place the gel directly on the surface of the Lighting Area Failing to use the tray may cause buffer leakage and damage the LED Figure 6 Slide the black plastic board into the groove of the white backlight to block the white light on the lid Figure 7 It is suggested to slide the black plastic board to block the white backlight on the lid for fluorescent stained gel scanning For colorimetric gel scanning leave the white backlight unblocked on the lid Close the scanner lid Slide the Light Selection Switch on the front side of the scanner to choose blue light source for scanning stained gel Set and adjust the desired image quality through the Functional Menu if necessary Please check Functional Descriptions section for the details of all functions Press SCAN button green LED will flash and CAPTURING will be displayed on the screen The scanned image will automatically save to SD card Scanning of the gel sample is now complete Black plastic board White backlight Figure 7 Slide the black plasti
10. ls of all functions 9 Press SCAN button green LED will flash and CAPTURING will be displayed on the screen The scanned image will automatically be saved to SD card Scanning of the blot is now complete FastBene GelPic LED Box BROWSE SCANNED IMAGES DELETE SCANNED IMAGES 1 Examine Scanned Images a When icon displays on top left corner of the screen press OK to get into functional options b When Scan Mode icon is highlighted press OK again to get into Play Mode c The Play Mode icon will display top left corner of screen The captured images can then be reviewed one by one by pressing right arrow button Delete Scannes Images a When viewing the image that needs to be deleted press OK to get into Function Descriptions II b Press right arrow button to choose Edit Mode 6 Press up down arrow button to choose Delete Press right arrow button to select yes no to delete the file d Press up down arrow button to select Yes Press OK to save the setting The image is thus deleted FastGene GelPic LED Box INSTALLATION OF AMBER FILTER BOARD FastGene GelPic LED Box is equipped with an amber filter board for blue light protection and gel cutting purposes Open the lid and install the filter board so the gel cutting task may be conducted 1 Openthe lid 2 Lift the lid to its maximum open angle 3 Position th
11. n to select the desired setting Press OK to save the setting Press down arrow button to select other setting mode or press OK again to return to Scan Mode Setup Menu and related setting items LCD Dark Normal Bright Display NTSC PAL NTSC USA PAL Europe Quality S Fine Fine Normal JPEG Compression Size 12M 9M 5M 1M Megapixels 9M is default setting Power Save 1 min 3 min 5 min 10 min off Power shut off Language English S88 rb c Format Yes No Format SD card Reset Yes No Reset all current settings back to factory default FastGene GelPic LED Box FUNCTIONAL MENU FUNCTION DESCRIPTION El b amp T zi Play Mode To play the captured images To examine the captured image m Auto Play Mode To automatically play the captured images one by one Edit Mode To edit the captured images such as to rotate or to delete the captured image etc thumbnail panel Set Up Mode Same as the Set Up Mode functions in Function Descriptions ER Preview Mode To preview the captured images in a multi image FastGene GelPic LED Box Mode When icon displays on top left corner of the screen press OK to get into functional options When Scan Mode icon is highlighted a press OK again to get into Play Mode The Play Mode icon will display top left corner
12. utton to choose light source Transmitted amp Epi Transmitted mode is to scan the gel stained with colorimetric stain such as the SDS PAGE gel stained with coomassie blue stain Press OK to save the setting FastGene GelPic LED Box ale eo Exposure Mode When icon displays on top left corner of the screen press OK to get into functional option list Press right arrow button to choose Exposure Mode E3 Press up or down arrow button to choose the desired exposure level When the desired exposure level is selected press OK to get back to Scan mode B W Switch Mode When icon displays on top left corner of the screen press OK to get into functional option list Press right arrow button to choose B W Switch Mode Press up or down arrow button to select the desired display mode color or B amp Wi color invert or B amp W invert The default setting of the scanner is Black amp White Press OK again to return to Scan Mode FastBene GelPicLED Box IT Up Mode When icon displays on top left corner of the screen press OK to get into functional option list Press right arrow button to choose Set Up Mode fil Press down arrow button to get into Setup Menu and select the desired setting item Press right arrow button to get into setting mode Then press up and down arrow butto

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