Nanomite Syringe Pump User`s Manual
Contents
1. the pump enters remote mode and responds with the three character sequence CR The prompt character indicates the status of the pump as follows prompt gt lt LF prompt meaning ASCII code When stopped ASCII 58 decimal When running forward ASCII 62 decimal Reverse Nanomite does not include stall detection Serial commands and their meanings Commands KEY RUN STP CLV CLT REV Return to keyboard control Exit remote mode Start infuse forward direction Stop motor Clears volume accumulator to zero Clears target volume to zero dispense disabled Start Reverse Direction Commands with numbers MMD ULM NLM ULH NLH TGT number number number number number number Set syringe diameter units are mm Rate is set to zero after MMD command Set flow rate and range units are microliters per minute Set flow rate and range units are nanoliters per minute Set flow rate and range units are microliters per hour Set flow rate and range units are nanoliters per hour Set target infusion volume units depending on flow rate range Numbers can be between 0 and 9999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded Publication 5414 001 REV B 7 Nanomite Syringe Pump Harvard Apparatus Appendix D Serial Communication Queries DIA Returns diameter value units in mm R2. Nanomite for changing from infuse to withdraw on the fly When the pump is infusing and the switch is toggled the pump will now be in the withdraw mode Switch the toggle again it puts it back into infuse mode The physical position of the switch does not matter The pump always powers up in infuse mode Maintenance Keep the pump clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw and guide rods It is important to keep these guide rods clean and lubricated To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol If the pump does not work properly contact Harvard Apparatus for appropriate instructions Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus Operation Advanced Features Remote Communication The Nanomite RS232 option is equipped at the factory for remote serial com munications The baud rate and address are set in the CONFIG mode options a Pump Address To set the pump address at the RATE VOL CONFIG display move the underline cursor using the Vor Ake to CONFIG Press the SET key to enter the CON FIG mode options Use the v or A key to scroll the CONFIG options until you reach the SET ADDRESS Press th
3. to select either mode Exit the CON FIG mode by pressing the ENTER key again and save the POWER ON setting in memory This feature is only for pump mode Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus Operation Advanced Features Display Intensity For varying light conditions four levels of intensity can be set on the vacuum flu orescent display To set the desired display intensity at the RATE VOL CONFIG display move the underline cursor using the Vor Ake to CONFIG Press the SET key to enter the CONFIG mode options Use the Vor A key to scroll the CONFIG options until you reach the SET INTENSITY Press the SET key again moving the under line cursor to the right Use the VorA key to scroll the SET INTENSITY choic es 1 2 3 4 4 is highest intensity 1 is the lowest intensity Press the ENTER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INTENSITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until a new rate is entered Except for volume mode the flow rate can be changed while the pump is running As soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in volume mode the pump must be stopped first Infuse Withdraw Switch Operation For customer convenience a two position rocker switch is included in the
4. 3 100 1 46 250 2 30 500 3 26 Hamilton Microliter Series Gastight 700 1700 1000 Series Size yl Diameter mm 0 5 0 103 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1000 4 61 Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus Appendix C Flow Rates nominal inside ni min syringe diameter size ul mm 0 5 0 10 3 300 9999 0 0600 894 8 0 0032 53 00 0 0001 0 800 1 0 15 7 374 9999 0 1230 2013 0 0074 120 8 0 0003 2 013 2 0 21 14 46 9999 0 2409 3946 0 0144 236 7 0 0004 3 946 5 0 33 30 69 9999 0 5948 9744 0 0357 584 6 0 0006 9 744 10 0 46 69 35 9999 1 156 9999 0 0694 1136 0 0012 18 93 25 0 73 174 7 9999 2 911 9999 0 1747 2861 0 0030 47 68 50 1 03 347 7 9999 5 800 9999 0 3477 5696 0 0058 94 93 100 1 46 698 6 9999 11 65 9999 0 6986 9999 0 0120 190 7 250 2 30 1734 9999 28 90 9999 1 734 9999 0 0290 473 3 500 3 26 3483 9999 58 05 9999 3 483 9999 0 0590 951 0 1000 4 61 6965 9999 116 1 9999 6 965 9999 0 1161 1901 This table reflects the minimum and maximum values programmable for the specified syringe diameters Publication 5414 001 REV B 5 Nanomite Syringe Pump Harvard Apparatus Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13
5. AT Returns rate value set in current range units VOL Returns current accumulated infused volume units in ml or ul depending on range VER Returns model and version number of firmware TAR Returns target volume units in nl or ul depending on range value format nnnn nnn The returned value is an 8 character string with leading zeros converted to SP characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal Queries With String Response RNG Returns range message character string either NL H NL M UL H UL M Error responses CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range Pump Chain Pump Port 1 in Computer control side Pump Port 2 out Connection for remainder of pump chain Baud Rate 300 1200 2400 or 9600 Word Size 8 Parity none Stop Bits 2 Publication 5414 001 REV B 5 Nanomite Syringe Pump Harvard Apparatus Appendix E Dual RS 232 TXD GND RXD GND GND TXD GND RXD IN OUT Host Chain Computer Connection to Connection Additional Pump s Daisy Chain Connector amp Cables Part No MA1 70 2022 MA1 72 2478 MA1 55 7760 MA1 72 8340 Description RS232 Serial Cable 6 ft long 9 pin d sub on one end and RJ11 on the other end Daisy Chain Cable 6 ft Daisy Chain Cable 2 ft USB to Serial Adapter Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatu
6. D WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact your local dis tributor or Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our cus tomer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not C A U T l O N for clinical use on human or veterinary patients It is FOR RESEARCH USE
7. Nanomite Syringe Pump User s Manual Nanomite Syringe Pump MAI 70 2217 HARVARD APPARATUS Publication 5414 001 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any change
8. ONLY intended for research use only NOT FOR CLINICAL USE ON PATIENTS Publication 5414 001 REV B s Nanomite Syringe Pump Harvard Apparatus General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Power Supply The pump is supplied with an approved power supply and line cord To maintain the safety integrity of the device use only one of the following power supplies Ault Inc Model PW118 Output 12 Vdc 1 5 A Input 100 250 Vac 50 60 Hz 5A Cui Inc Model SAO6ON12 V Ouptut 12 Vdc 2 0 A Input 100 240 Vac 50 60 Hz 8A Globtek Inc Model GT 4201D 12 Output 12 Vdc 1 66 A Input 100 240 Vac 50 60 Hz 0 6A Use Proper Line Cord Use only the line cord shipped with the product and make sure line cord is certified for country of use Ground the Product This product is grounded through the return path of the DC power supply To avoid electric shock use only approved power supply and line cord with the product Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all co
9. and withdraw Press RUN Press the RUN STOP key to start pump and begin pumping The Run Indicator Blue LED located in mechanism box near syringe barrel will light when the pump is on and pumping Check Syringe Often The Nanomite will shut itself off via the volume mode It will not shut off in pump mode Although this presents no hazard to the user or the pump it is prudent to check the syringe from time to time Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus Operation Advanced Features Volume Mode The Nanomite can be set to dispense a precise volume and then stop To acti vate the volume dispense mode a target volume must be set To set a target volume at the RATE VOL CONFIG display move the underline cursor using the VorA key to VOL Press the SET key to enter the VOL set mode Use the s or t key and the SET key to display a target volume from 00 01 to 99 99 Volume units are either nl nanoliter or ul microliters Target volume units are established in the CONFIG SET UNITS mode example if your pumping units are ul min or ul hr then the volume dispense units will be ul s Press the ENTER key to select the desired target volume Exit the VOL mode by pressing the ENTER key Once you press the RUN key the pump will run until the target volume is deliv ered The display will show the actual volume dispensed along with the target volume Press the RUN key each time you want to repeat the volume
10. dispense If you press the STOP key during a volume dispense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 00 or turn off and on the pump via the main power switch NOTE In the event of a power fail ure the actual dispensed volume and the target volume are not retained in mem ory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if the POWER ON mode was set to run This mode will function for both infuse and withdraw Blue LED mounted in mechanism will flash as it dispenses to target Beeper will sound three times at the end of volume dispense once target is reached Power Failure In the event of a momentary or prolonged power failure the Nanomite can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with RATE VOL CONFIG SELECT MODE on the display To set the power fail mode at the RATE VOL CONFIG display move the under line cursor using the V or Ake to CONFIG Press the SET key to enter the CONFIG mode options Use the y or key to scroll the CONFIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to the right Use the Arv key to scroll the POWER ON choices stop or run Press the ENTER key
11. e SET key again moving the underline cursor to the right Use the VorA key to scroll the two digit number fields Use the or key and the SET key to display a unique pump address from 00 to 99 Press the ENTER key to select the desired pump address Exit the CONFIG mode by pressing the ENTER key again and save the ADDRESS setting in memory b Baud Rate To set the pump Baud Rate at the RATE VOL CONFIG display move the under line cursor using the Vor Ake to CONFIG Press the SET key to enter the CONFIG mode options Use the y or A key to scroll the CONFIG options until you reach the SET BAUD Press the SET key again moving the underline cursor to the right Use the y or A key to scroll the SET BAUD choices 300 1200 2400 or 9600 Press the ENTER key to select the desired baud rate Exit the CONFIG mode by pressing the ENTER key again and save the BAUD setting in memory Press RUN Or Foot Switch Press the RUN STOP key or press the foot switch to start pump and begin pump ing The Run Indicator Blue LED will light when the pump is on and pumping FAST FORWARD FAST REVERSE Press simultaneously the run and the A key or the run and y key to actuate the fast forward or fast reverse mode respectively The pump travels at its maxi mum rate while both keys are pressed simultaneously Publication 5414 001 REV B 5 Nanomite Syringe Pump Harvard Apparatus Appendix A Nanomite Specifications Type Sy
12. earance around the pump e Appropriate environmental conditions e A well ventilated room Loading the Syringe Using the Fast Forward Fast Reverse feature position the Pusher Block to accept the loaded syringe Pull the retaining knob up and set the syringe into the holding clamps for the barrell flange and plunger Release the knob and insure that the pin rests on top of the syringe barrel Publication 5414 001 REV B 5 Nanomite Syringe Pump Harvard Apparatus Operation Getting Started Getting Started 1 Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate Nanomite and Software version 9 0 UGS Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right RUN STOP This turns the pump motor on and off ENTER This key enters the data that is on the display into the memory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to select which digit of the display is to be changed to move the decimal point and to move between modes Each time the set key is pressed the underline cursor below the digit or character on the display moves one step to the right It is used in conjunction with the ascending and descend ing keys When it the display shows the desired the correct value the set ke
13. into memory Enter Flow Rate Range Choose your flow rate units either microliters or nanoliters per minute or per hour From the initial RATE VOL CONFIG menu using the Vor A key move the underline cursor under the CONFIG mode menu prompt Press the SET key to enter the CONFIG mode Press the SET key again to move the underline cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the VorA keys to scroll the four flow rate choices nl min ul min nl hr ul hr Once the desired flow rate units are displayed press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return to the RATE VOL CONFIG menu These units will be the same for infuse and withdraw NOTE Small syringes must be used in order to access the nL hr range Enter Flow Rate From the initial RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on the display The underline cursor will appear under the left most digit or decimal point The V and keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired rate is displayed press the ENTER key to place this value into memory This value will be the same for infuse
14. m the syringe barrel Using the fast forward fast reverse feature position the pusher block so that the syringe plunger and syringe barrel will fit into the syringe holder and pusher block Place the syringe barrel in the V of the syringe holder making sure that the flange of the syringe barrel is positioned between the two upright posts and lower the syringe plunger knob into the pusher block Rotate and release the syringe clamp to hold the syringe in place Syringe Clamp Pusher Block Removable Mounting Rod Syringe Clamp Pusher Block Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus Installation Initial Setup 1 Read the manual to become familiar with all features and functions of the Nanomite 2 Connect the external DC Power Supply and line cord to the pump and main supply 3 Connect the 6 cable to control box and remote mechanism Observe orientation and polarity of the connector 4 Turn on main power switch located on the rear panel The display will now illuminate indicating that the power connections are correct Location Requirements for the Syringe Pump e A sturdy level clean and dry surface e Minimum of one inch 2 5 cm cl
15. nd Maximum Flow Rates 15 D Serial Communications Commands Queries and Responses nnnn nnen eneneneenvenennenen ee vennenenenen en 16 17 E Serial Port Connections Dual RS 232 nnn nnee eneen 18 Daisy Chain sissit i eveneens 18 F Nanomite to PC Connection nennen rer 19 Publication 5414 001 REV B Nanomite Syringe Pump Harvard Apparatus General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibration All syringe pumps are designed and manufactured to meet their performance specifi cations at all rated voltages and frequencies A calibration certificate is available upon request Contact customer service for details and pricing Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIE
16. nnections Publication 5414 001 REV B 5 Nanomite Syringe Pump Harvard Apparatus General Safety Summary Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION l Functional Ground Refer to Manual Terminal Enviromental Conditions Indoor use only e Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH e Well Ventilated Room e Altitude up to 2000 m e Mains Voltage Fluctuation not to Exceed 10 of Nominal Transient Overvoltage Category II e Pump is Rated Pollution Degree 2 in Accordance with IEC 664 Publication 5414 001 REV B s Nanomite Syringe Pump Harvard Apparatus Introduction Theory of Operation The Nanomite is designed as a low cost single infusion pump The Nanomite is stan dard with serial communication dual RS232 reversing switch and foot switch The Nanomite consists of a control unit an injection unit and a 6 ft cable The pump can hold syringes of any make from 0 5 ul to 1000 ul The internal diame ter of the syringes is entered via the keypad and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow Nonvolatile memory st
17. ores the last syringe diameter and flow rate along with other configuration data The Power Failure Mode can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright Display and Easy To Use Interface A two line 16 character vacuum fluorescent display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries required to start pumping syringe Inside Diameter mm and pumping flow rates The Flow rate can be changed while the Pump is running Two Modes of Operation Constant Flow Rate amp Volume Dispense The Nanomite will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode Smooth Flow Enhanced micro stepping pump profiles deliver very smooth and consistent flow that is virtually pulse free Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved The Nanomite meets all relevant European EMC and Safety requirements for labora tory equipment Voltage selector switch and fuse for 110 220V Publication 5414 001 REV B 4 Nanomite Syringe Pump Harvard Apparatus Nanomite Injector Unit Front and Rear Views Loading The Syringe Raise the spring loaded syringe clamp and rotate away fro
18. ringe Size for Nanomite with Single Syringe Holder Flow Rate Range Minimum Maximum Flow Rate Accuracy Calibration Display Nonvolatile Memory Maximum Force Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Input Power Input Power Connection Power Supply Control Unit Size HxWxD Weight Injector Unit Size HxWxD Weight Publication 5414 001 REV B Microprocessor single syringe Glass From 0 5 ul to 1000 ul 3 3 nl hr with 0 5 ul syringe 1901 ul min with a gastight 1 ml syringe 0 5 Automatic enter syringe size up to 6 00 mm Inside Diameter 2 line 16 character vacuum fluorescent display and blue run LED Stores diameter rate and configuration settings 11 to 12 lbs 1 8 step angle 1 pulse in 27 6 sec 200 steps sec 0 8 um pusher advance per motor step 7 0 um min 114 mm min 1 to 16 384 Typically lt 150 pA Typically lt 0 05 Q 12 VDC 1 5 Amps 2 5 mm ID x 5 5 mm OD male plug 100 250 VAC 50 60 Hz 18 Watts Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 8 9 x 22 9 x 11 4 cm 3 1 2 x 9 x 4 1 2 in 1 7 kg 3 80 Ib 1 0 x 18 5 x 5 0 cm 2 1 2 x 7 1 4 x 2 in 0 36 kg 0 80 Ib 5 Nanomite Syringe Pump Harvard Apparatus Appendix B Syringe Inside Diameter SGE Scientific Glass Engineering Size ul Diameter mm 25 0 73 50 1 0
19. s Appendix F PC Connection m em PE RJ11 Cable i Straight Thru DB9 Female to Computer Nanomite Pinning 4 6 PIN RJ11 12 such as Syringe Pump Max Distance L COM P N RBA098F 15M Front View Computer Terminal Port Chart Settings Connection RJ12 6x6 RJ11 RJ12 STD DB9 Baud Rate 9600 2 RD or match pump baud rate 3 TD Data Bits 8 2 5 Sig ground Stop Bits 2 RJ11 6x4 View of Cable Adapter Figure 10 Nanomite to PC Connection Publication 5414 001 REV B Declaration of Conformity Application of Council Directive s Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Type of Equipment Model No 73 23 EEC 89 336 EEC EN61010 1 1993 A2 1995 EN 61326 1997 Al 1998 Class A Emissions Class B Immunity Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 USA Syringe Pump Nanomite I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Date CE HARVARD APPARATUS United States of America April 1 2005 Signature Robert D Bohne Full Name VP Engineering Operations Position
20. s as they apply Do Not Dispose Product with Municipal Waste e Special Collection Disposal Required 5 Nanomite Syringe Pump Harvard Apparatus Table of Contents SUBJECT PAGE NO General Information Warranty and RepairS ecessssseeeees 2 General Safety Summary cesser nn 3 4 Introduction Theory of Operation nn nnen eenn renen ren enne ken nen erne reen nen 5 F amp atUfES arr nni a danetienerwe nnn aant eee R RN 5 Nanomite Front and Rear Views anneer eneen eenen 6 Installation Initial Setup amp Location Requirements nnen eenen 7 Loading the Synge as ra ole eee eed elle 7 Operation Getting Started T r Pump ON aannames tanend onbetekenend bdapansnadntins Function Keys and Run Indicator Entering Syringe Size nn Entering Flow Rate Range Entering FOW Fale nanne nnn dete POSS RUD renin a indenken CHECK SVINJE desierit nett A aen 9 Advanced Features VOLUME ModE sara hist ayo eee 10 Power Falun sessies a nani 10 Display Intensity nnen eeneneneereennnnneenrennenenenennennenennn 11 CHANGING ROS reinen 11 Infuse Withdraw Switch nnnnnrnenneneneenenennenee rennen 11 WEE te D EET E AR 11 Remote COMMUNICATION nnen sennnnenrerenennenee ren eennenn 12 Foot SWICM SEE EET DELE Ed 12 Fast Forward amp Fast Reverse nennen eenen 12 Appendices A Specifications ueneno 13 B Table of Popular Syringe Diameters naren enn 14 C Table of Minimum a
21. y will advance right to the next digit A V re ascending and descending keys are used to change the numbers on the display Up key makes numbers increase Y Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible blue LED near syringe mounting blocks will illuminate This LED will be on when in the pump ing mode and will flash when in the volume dispensing mode Publication 5414 001 REV B s Nanomite Syringe Pump Harvard Apparatus Operation Getting Started w A Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm Max setting is 6 0 mm If you do not know your syringe diameter refer to appendix B for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Press SET followed by the DIAM key The previously used diameter will appear on the display The underline cursor will appear under the left most digit or dec imal point The A ana V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired diameter is displayed press the ENTER key to place this value
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