MIT1000 User`s Manual - Micro Imaging Technology, Inc.
Contents
1. Micz2o DENTIFICATION i ECHNOLOGIES MIT 1000 Rapid Microbial Identification System User Guide Micro Identification Technologies 970 Calle Amanecer Ste F San Clemente CA 92673 Tel 949 388 4546 Fax 949 388 4547 www micro identification com e mail info micro identification com MIT 1000 System User Guide V3 5 Copyright 2007 by Micro Identification Technologies Inc All rights reserved Micro Identification Technologies believes the information furnished in this document to be accurate and reliable However Micro Identification Technologies assumes no responsibility for its use or for any infringements of patents or other rights of third parties which may result from its use No license is granted by implication or otherwise under any patent rights of Micro Identification Technologies Use duplication or disclosure by the United States Government is subject to restrictions as set forth in subparagraph c 1 ii of the Rights in Technical Data and Computer software clause at 48 C F R 252 227 7013 or in subparagraph c 2 of the Commercial Computer Software Registered Rights clause at 48 C F R 52 227 19 as applicable Micro Identification Technologies 970 Calle Amanecer Ste F San Clemente CA 92673 MIT 1000 and Identifier is a trademark of Micro Imaging Technology Inc All other brand and product names used in this document are trademarks or registered trademarks of their respective companies MI2. E coli Listeria spp Salmonella spp Test Details Test Time 2012 10 23 09 40 37 User G Kessler Termination Normal Sample Details Gram stain result Not performed Header Text Print Click Print to print a copy Figure 6 Sample Test Report Partial View MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Note A full view of the Sample Test Report is shown in Appendix C Test Report Header The text in the Header Text box will be displayed at the top of the Test Report Follow the procedure below to customize the header e Enter the header text in the Header Text box and then click Apply e To remove the header delete the text in the Header Text box and then click Apply MIT 1000 System User Guide V3 5 Configuring the System Micro DENTIFICAT ION ECHNOLOGIES Click the Configure button to display the MIT 1000 Configuration Options Form as shown in Figure 7 A description of the options is shown in Table 2 MIT 1000 Serial Number Optional Log entries List of Culture Medias Optional Test Report Test Options arameters P List of Users T pen fie MIT 1000 S N 000020 Medium Users M Chime on completio TSA Dave Diagnostic Tools Require reason for ser TSA wi5 SB Termination Required Log Entries Show in Report I Media I Customer Name I Media Lot Number I Project Number j e I Media Expiration Date PO Number I Incubation Te
3. Repeat steps c through d five times 2 Fill vial with LPF Water as shown in Figure 20 When properly filled the vial neck should be about half full The System will operate properly as long as the level is above the vial neck bottom and below the top lip Figure 20 Fill the MIT Vial to the indicated Fill Level 7 Current MIT approved medium is BHI or TSA with 5 sheep blood MIT 1000 System User Guide V3 5 Micro IDENTIFICATION p ECHNOLOGIES 3 Immediately center the Parafilm square with the paper protected side down over the top of the vial and press it into place forming a seal with the outside of the neck as shown in Figure 21 Secure by double wrapping the vial neck with a 10 rubber band Lift up the sides of the Parafilm away from the round bottom 4 Perform a Water Quality Test as described in Chapter 5 Note Perform Steps 2 amp 3 within 1 hour after completing Step 1 Step 2 Clean the Micro Centrifuge Tube Items Needed e 0 6 mL micro centrifuge tube e LPF Water Procedure Using LPF Water 1 Rinse the outside of the tube first starting from the bottom 2 Rinse the inside of the tube by repeating the following sequence at least five times a Completely fill the tube with LPF Water b Turn the tube upside down and VIGOROIUSLY SHAKE out all of the water Step 3 Fill the Micro Centrifuge Tube with Water Items Needed e 0 6 uL micro centrifuge tube e LPF
4. Figure 33 the SCG will point in the blue zone and the Concentration indicator will blink blue If this condition persists stop the identification process by clicking Stop Identify and prepare a new sample Note If the gauge pointer drifts in and out of the blue zone the test will likely be performed roperly but could take several minutes longer than usual to complete Power O MIT Cloud when the Concentration light is MIT 1000 Ready blue and blinking the sample concentration is too low Concentration Check Vial E coli Listeria spp Salmonella spp The Sample Concentration Gauge indicates the sample concentration is low Troubleshooting Configure MIT Web Stop Identify Figure 33 Low Concentration Condition MIT 1000 System User Guide V3 5 M ICRO Joenr IFICATION ECHNOLOGIES High Concentration Indication When the sample concentration is too high Figure 34 the SCG indicator will point in the right side red zone and the Concentration Indicator will begin blinking red If this condition persists stop the identification process by clicking Stop Identify and prepare a new sample with less concentration N ote It is permitted to have the gauge pointer drift into and out of the red zone MIT 1000 RMID Power O Micro Identification echnologies MIT Cloud When the Concentration light is MIT 1000 Ready rea and b
5. Troubleshooting Configure MIT Web Stop Identify Figure 35 Check Vial Display during a Water Quality Test or Identification Test MIT 1000 RMID Micro Identification Power echnologies MIT Cloud MIT 1000 Ready Active Identifiers User Guide pe When the MIT 1000 Application detects vial glare it terminates and displays the Vial Glare warning Troubleshooting ante Terminated vial glare Figure 36 The MIT 1000 Application terminates the Water Quality Test or Identification Test and displays the Vial Glare warning when it detects vial glare MIT 1000 System User Guide V3 5 Micro DENTIFICATION ECHNOLOGIES Chapter 8 Troubleshooting Condition Probable Cause Recommended Action MIT Cloud Light blinks red e The System has no 1 Verify that the MIT 1000 Computer has connection to the network access internet 2 Configure your firewall to allow the MIT e Your network Firewall 1000 Application to have access to prohibits the MIT 1000 TCPIP Ports 12000 12010 Application from communicating on port 12000 12010 Ready Light blinks red e System is not powered 1 Verify that power on the MIT 1000 or cables are not System is ON properly connected 2 Verify cables are connected properly e The MIT 1000 System 3 Turn on the power to the MIT 1000 was powered on after System then Restart the MIT 1000 the MIT 1000 Application Application was started 4 if item
6. e Micro centrifuge tube from Step 3 Procedure While holding the inoculating loop that contains the organism cells 1 Hold the water filled micro centrifuge tube in one hand 2 Open the tube and tilt it no more than 45 making sure not to spill the contents 3 Insert the inoculating loop into the water in the micro centrifuge tube and dislodge the sample into the water by agitating the loop in the water as shown in Figure 24 Dislodge the Organism Figure A 9 g P Cells into the Water in the Micro centrifuge Tube 4 Withdraw the loop and immediately close the tube 5 Properly dispose of the contaminated loop in a Biohazard Bag Step 6 Vortex the Sample Items Needed e Micro centrifuge tube with the organism cells in dilution from step 5 e Vortex mixer Procedure 1 Vortex the micro centrifuge tube for at least 20 seconds Figure 25 2 Proceed directly to Step 7 Note To ensure optimum performance do not delay proceeding to Step 7 Figure 25 Vortex the Micro centrifuge Tube MIT 1000 System User Guide V3 5 Micro IDENTIFICATION p ECHNOLOGIES Step 7 Prepare the MIT Vial Suspension Items Needed e Micro centrifuge tube with vortexed organism cells suspension e 0 5 10uL adjustable pipette with tip e Covered MIT Vial containing LPF Water from Step 1 Procedure 1 Always vortex the micro centrifuge tube immediately before this step 2 Remove the rubber band from the MIT
7. 20 Clean and rinse a MIT Vial with LPF water see Chapter 4 Add about 15 mL of LPF Water to the MIT Vial as shown in Figure 20 on page 29 The water level must new the middle of the vial neck Cover with Parafilm and secure with rubber band Clean and rinse a 0 6 mL micro centrifuge tube with LPF water Fill the micro centrifuge tube to about 0 2 mL with LPF water Remove a single colony from a culture plate with a 1 uL sterile inoculating loop Dislodge the colony material on the loop into the water in the micro centrifuge tube Close the micro centrifuge tube Vortex the micro centrifuge tube Remove the rubber band and gently lift the Parafilm covering the MIT Vial and pipette 1 ul from the micro centrifuge tube into the MIT Vial Re cover the MIT Vial with Parafilm and replace the rubber band Lift up the tails of the Parafilm to get the Parafilm off the neck Wipe the round bottom of the MIT Vial with a Kimwipe dampened with 70 IPA Wipe dry the round bottom of the MIT Vial with a second Kimwipe Place the MIT Vial in the MIT 1000 System Start the MIT 1000 Application Click the Identifiers button and select the appropriate Identifiers to use for the identification test Select IDENTIFY Fill in the Sample Identification form Click OK MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Chapter 3 MIT 1000 Application There are three ways to start the MIT 1000 Application M IT e
8. Module MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Equipment and Materials Required For an Identification Test Equipment Required e MIT Vial holder MIT Part Number 30003 e Vortex mixer See specifications in Appendix B e Pipette 0 5uL to 10 uL See specifications in Appendix B e Incubator e Autoclave e BioSafety Cabinet Material Required e Low Particle Filtered LPF water e 0 6 mL micro centrifuge tube See specifications in Appendix B e Parafilm See specifications in Appendix B e 1 uL sterile disposable loop See specifications in Appendix B e 10 rubber band e Kimwipes e Pipette tips See specifications in Appendix B e MIT Vial MIT Part Number 20007 e 70 IPA e 10 solution of Distilled White Vinegar e Powder free gloves e Trypticase Soy Agar with 5 sheep blood or BHI culture plate e Biohazard disposal container 3 Low Particle Filtered Water Typically this water is the product of a water filtering system with a final filter of 0 1 micrometers absolute or smaller The MIT 1000 Water Test function is the final decider on whether or not water can be used for Identification tests with the MIT 1000 System 5 to 8 acetic acid in water with a pH of about 2 4 Available at most grocery stores MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Chapter 2 Quick Reference Identification Test 1 sO MPNOARY 11 12 13 14 15 16 17 18 19
9. Water Procedure 1 Immediately after rinsing the tube fill the tube with about 0 2 mL about 1 3 full with LPF Water Figure 22 MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Fill the tube with 0 2 mL of LPF Water Figure 22 Micro Centrifuge Tube with 0 2 mL of LPF Water 2 Snap the micro centrifuge tube cover closed Step 4 Collect a Test Sample Items Needed e Bacteria in isolated colonies e 1 uL sterile inoculating loop e Biohazard Bin and Biohazard Bag Procedure Examine the culture plate Note Cultures should be less than 48 hours after first appearance on culture plate and select the isolated colony to be identified Then Single colony 1 Place the culture plate upside down lid down in the BioSafety Cabinet 2 Using aseptic techniques hold the culture plate in your hand and slowly lift the culture plate off the lid 3 Remove a small portion of a single colony by lightly placing the loop at the edge of the colony and dragging Figure 23 Collecting a Test the loop through the colony to collect organism cells as Sample shown in Figure 23 Note Avoid collecting culture media Put the culture plate back on the lid Immediately proceed to Step 5 ore MIT 1000 System User Guide V3 5 Micro Joenr IFICATION ECHNOLOGIES Step 5 Prepare the Micro Centrifuge Tube Suspension Items Needed e Inoculating loop containing organism cells
10. edit For example TSA with 5 SB can be changed to Blood agar Table 3 Media Configuration Options Upon clicking the New or Edit button associated with Media the Form shown in Figure 8 will open To add a new or edit the Culture Media 1 Enter the new name 2 Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 8 Media Name MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES User Configuration To add a User s Name follow the instructions in Table 4 below User List The User list box shows the available names that the User can select prior box to starting an Identification Test New Click New to add a new User option When the User Name Form Figure 9 appears fill in a name for the new User Then click OK to accept the new User or click Cancel to close the form without accepting the new User Edit Click Edit to modify the User name When the User Name Form Figure 9 appears the current User Name is filled in Modify the name and click OK to accept the change or click Cancel to close the form without accepting the change For example Susan B Anthony can be changed to SBA Table 4 User Configuration Options Upon clicking the New or Edit button associated with User the Form shown in Figure 9 will open To add a new or edit the User Enter the new
11. hold it in place with a 10 rubber band then store it on an MIT Vial Holder MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Chapter 5 Water Quality Test The MIT 1000 System requires LPF water for proper operation MIT recommends that a water quality test be performed before every Identification Test The water filled MIT Vial can be used for Identification Tests only if it passes the 30 second Water Test If a test failure occurs dump the water in the vial then clean rinse and fill the vial as described in Step 1 of Chapter 7 on page 29 To perform a Water Test 1 2 Clean rinse and fill the MIT Vial as described in Step 1 in Chapter 7 on page 29 Place the MIT Vial in the MIT 1000 System as described in Step 9 in Chapter 7 on page 34 and click Water Test as shown in Figure 16 Power MIT Cloud MIT 1000 Ready m Active Identifiers Click Water Test to begin a water quality test Log Configure Figure 16 Water Test Upon clicking the Water Test button the following changes occur in the MIT 1000 Application window Both the Identify and Close Program buttons are removed from the dialog The word Busy with a flashing green light appears The Progress Bar appears After a few seconds the Sample Concentration Gauge SCG appears The indicator in the Gauge should remain at the lowest condition see Figure 17 during the test 7 The progress b
12. name Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 9 User Name Information Entry Customer Configuration To input Customer information follow instructions in Table 5 below Customer List Click the Down Arrow to show the list of Customers that have already been box entered Selecting a Customer will allow the entered Projects for that Customer to be viewed or changed and new Projects added The selected Customer is also shown in the lower left of the form New Click New right of the Customer list box to add a new Customer to the list When the Customer Form Figure 10 appears fill in the Customer Name and enter any comments or notes on the Customer Then click OK to accept the new Customer or click Cancel to close the form without accepting the new Customer Edit Click Edit right of the New button to modify the selected Customer When the Customer Form Figure 10 appears the Customer Name and Notes fields will be filled in with the selected Customer s information Modify the fields as needed and click OK to accept the changes or click Cancel to close the form without accepting the changes Table 5 Customer Configuration Options MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Upon clicking the New or Edit button associated with Customer the Form shown in Figure 10 opens
13. 1000 Application eerrnnvrnnnnvnnnnnnnnnvvnnnnvennnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnenn 10 MIT 1000 Application Display v uvvasvvm aner mera ccelivandaecith acces ata add bt 10 MIT 1000 Application Display Button Functions uarnvvrnnennvvrnnnnnvvrnnnnnnnnnnnnnnnrnnnnrnnrnnerrnnnn 12 Logs and Repons raisti a en eeunte AESA EA lave E E A 13 Chapter 4 MIT Vial Cleaning and Storage mmsrnnavvnnnnvnnnnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 25 sterilizing the MIT Vialuuusuoemasunstdaankdsuumnumiumuabbnvmuttie 25 MIT Vial Cleaning sii 2s ievih ete ian EG om alae 25 storing MIT Vials iiiu a een ane ae el 26 Chapter 5 Water Quality Test rrnnvnnnnvrnnnnvnnnnnnnnnvvnnnnvennnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnenn 27 Water Test ReSUltSin annndesturnehedenenmdie seen euetedva ides niende 28 Chapter 6 Sample Preparation rrevnnnnvrnnnnvnnnnnnnnnvnnnnnvennnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 29 Step 1 Fill the MIT Vial with Water sseronnnnnnrrnannvonnnnvnnrnrennnrrnnnnrnnenrnnnenrennnrnesnnnnnennenennne 29 Step 2 Clean the Micro Centrifuge Tube cccseeeeeeeeeeeeeceeeeeeeeceeeeeseaeeesaeeeeaeeeeeeeeaas 30 Step 3 Fill the Micro Centrifuge Tube with Water smarnrnnnnronnnnvnnnnvvnrerrvnnnrrnnnnvnnrnnnnennn 30 Step 4 Collect a Test Sample rnnrnnannvnnnnnvnnnrrnnnnvnnnnnvnnvnvvnnnrnnnnnnnnannennenrennnrnsennnenennenennne 31 Step 5
14. 5 M ICRO DENTIFICATION ECHNOLOGIES Identification Test Operation During the Identification Test the MIT 1000 Application Display changes to show test status and progress Figure 31 Micro Identification wil echnologies MIT Cloud MIT 1000 Identification Test progress indicator Sample Concentration Gauge Active Identifiers Check Vial E coli Listeria spp Salmonella spp Troubleshooting Doing an Identification test indicator Configure s Manual stop identification process MIT Web Stop Identify button Figure 31 Identification Test Progress Display The Busy indicator appears and flashes green The Identification Test progress indicator appears and will change as the test progresses The SCG provides a real time estimate of the concentration of the sample being tested If the indicator is in the blue or red zone it is recommended that the User take action as described in the Indicator Explanations in the next section The Identify button changes to a Stop Identify button Click the Stop Identify button to stop and cancel the Identification Test at any time during the identification process The Close Program and Water Test buttons are hidden When the Identification Test completes the Display reverts to its original state Figure 32 and the sample is identified in bold blue letters above the Close Program button If the sample is an Species Genus or Subs
15. 6 2012 09 07 09 15 21 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000235 2012 09 10 09 55 47 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000357 2012 09 11 09 35 25 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000378 2012 09 12 09 47 41 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000397 2012 09 13 09 21 14 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000425 2012 09 14 09 32 52 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF m View Report Add Comment Click View Report to show a Report for the selected test the selected test Click Add Comment to add a comment to Figure 5 Sample Test Log Display MIT 1000 System User Guide V3 5 M ICRO IDENTIFICATION ECHNOLOGIES R Recorded Log Information Source of information MIT 1000 A unique index assigned to an Identification Test by the MIT 1000 Test er Application Application program MIT 1000 TE Time eee The time and date the Identification Test started Application Sample name or type MIT 1000 eens Application The result of the Identification Test The result of a Gram Stain performed on the sample The possible values are Not done Gram Stain User GNR Gram Negative Rod GPR _ Gram Positive Rod GNC Gram Negative Cocci GPC Gram Positive Cocci Yeast The organization or company that provided the sample being tested Sud The Project Number as
16. Application Display Button 19 Functions MIT 1000 Application User Interface 22 Button Functions 20 MIT Products MIT Vial 12 Cleaning 31 Placement 5 Sterilizing Storing 8 Suspension Preparation Sample 37 Collection Preparation 37 Vortex 36 Sample Test Report 35 Soapy water System Set up Test 37 Identification 37 Water Quality Troubleshooting 13 Underlined sentence User Guide Conventions Notes Warnings 13 Warnings 15 16 32 12 10 42 25 34 25 26 33 31 29 32 43 45 35 27 40 aN MIT 1000 System User Guide V3 5
17. Customer esj To add a new or edit the Customer Enter the new name Enter any notes or comments Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 10 Customer Information Entry Project Configuration To input a Project Name follow the instructions in Table 6 Project List box Click the Down Arrow to show the list of Projects that have already been entered for the selected Customer Selecting a Project will allow the entered Tasks for that Project to be viewed or changed and new Tasks added The parameters for the selected Customer and Project are shown in the lower left of the form New Click New right of the Project List box to add a new Project for the selected Customer to the list When the Project Form Figure 11 appears fill in the Project Number and or Purchase Order Number and enter any comments or notes on the Project Then click OK to accept the new Project or click Cancel to close the form without accepting the new Project Edit Click Edit right of the New button to modify the selected Project When the Project Form Figure 11 appears the Project Number PO Number and Notes fields will be filled in with the selected Projects information Modify he fields as needed and click OK to accept the changes for click Cancel to close the form without accepting the changes Table 6 Project Information
18. Double click the MIT 1000 Application icon Figure 1 on the desktop 1000 e Right click on the desktop MIT 1000 Application icon and select the Open menu item Figure 1 MIT e Click on MIT 1000 Application icon in the MIT folder in the All Programs 1000 Application menu under the Start button Icon MIT 1000 Application Display The MIT 1000 Application Display as shown in Figure 2 and 3 appears after the MIT 1000 Application has started MIT 1000 RMID Micro Identification why Power on hnologi MIT Cloud Display and select Identifiers ere e MIT 1000 Ready Active Identifiers Listeria spp Display this User Guide PDF Display the troubleshooting guide Display the test log Display the MIT 1000 configuration page Troubleshooting Display the MIT web page s N Figure 2 MIT Central Control Display WaterTest Identify The six buttons on the left side of the Display provide an easy method to configure the MIT 1000 Application They provide the User with the capability to troubleshoot review test log data configure the MIT 1000 System and connect to the MIT 1000 website for technical updates and other announcements Click the Identifiers button to select the Identifiers to use for Identification Testing Click the User Guide button to display this User Guide Click the Troubleshooting button to display the Troubleshooting Guide Click the Log button to display the Sample Test Log Click the Config
19. Entry MIT 1000 System User Guide V3 5 Upon clicking the New open Micro IDENTIFICATION ECHNOLOGIES or Edit button associated with a Project the Form shown in Figure 11 will Project Project Number PO Number Notes To add a new or edit the Project 1 Enterthe new Project Number and or 2 Entera Purchase Order Number 3 Enter any notes or comments Task Configuration To input a Task Name 4 Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 11 Project Information Entry follow the instructions in Table 7 Task List box Click the Down Arrow to show the list of Tasks that have already been entered for the selected Customer and Project Selecting a Task will allow the entered Samples for that Task to be viewed or changed and new Samples added The parameters for the selected Customer Project and Task are shown in the lower left of the form New Click New right of the Task List box to add a new Task for the selected Customer s Project to the list When the Task Form Figure 12 appears fill in the Task Number and or Lot Number and enter any comments or notes on the Task Then click OK to accept the new Task or click Cancel to close the form without accepting the new Lot Number Edit Click Edit right of the New button to modify the selected Task When the Task Form Figure 12 appear
20. OLOGIES Chapter 1 Getting Started You will receive the MIT 1000 System in two boxes Inside the Large Box The large box contains the MIT 1000 System and Starter Kit The following items are included in the large box e MIT 1000 System Power Supply Module USB Cable Key Starter Kit The starter kit provides the items needed to use immediately the MIT 1000 System The kit includes the following items Oo Twelve 0 6 mL micro centrifuge tubes Twenty five sterile inoculating loops Six MIT Vials 10 MIT Vial Caps 12 Eppendorf 0 5 10 uL pipette tips 15 2 x 2 Parafilm squares One MIT Vial Holder 15 10 rubber bands One small vial of 1 59 micron PSL sphere suspension 00000000 Inside the Small Box The small box contains the MIT 1000 Mini Laptop computer The following items are included in the small box e The MIT 1000 Mini Laptop computer e Computer battery e Power module 1 59 Micron PSL Sphere Suspension Refrigeration Immediately refrigerate the 1 59 micron PSL sphere suspension 1 MIT Part Number 20007 MIT Part Number 30003 is used for both vial cleaning and ID Test preparation MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES System Set up Connect the MIT 1000 System Personal Computer and External Power Supply as shown in the diagram below MIT 1000 Computer Power Module USB Cable LASER RADIATION AVOID DIRECT EXPOSURE TO BEAM MIT 1000 Power
21. Prepare the Micro Centrifuge Tube Suspension cccceeeeeeeeeeeeeteeeeeeeteeeeeeees 32 Step 6 Vortex the Sample rrrrnnnnrnnannvnnonvvnnnrrnnnnrnnnnnvnnenvennnrnnnnnnnnannennenresnnrnsennnnnsnnenennn 32 Step 7 Prepare the MIT Vial SUSPENSION ccceeeeeeeceeeeeceeeeeeeaeceeeeeeeaeeesaeeneaaeseeeeeeaas 33 Step 8 Clean the MIT Vial Outside Surface cccecececeeeeeceneeeeeaeeeeeeeeeeeeeseaeeeeaaeseeeeeeeas 33 Step 9 Place the MIT Vial into the MIT 1000 System rrnrnrrnnnnrnnonvvnrnrrvnnnrrnnnnvnnrnnnnennne 34 Chapter 7 Performing an Identification TeSt rnmrnnnnvnnnnnvnnnnvnnnnvennnnvnnnnnnnnnnvnnnnvnnnnnnnnnnnnnnnnnnnnnenn 35 MIT 1000 Application Start Up srrrennrrnonnvnnenrennnrrrnnnrnnrnnnnrnrrennr renn nnnnennenrrrnesnnnnsennnnrennen 35 Identification Test Sample Information srrnrnrrrnnnrnnnnnvnnrnvnnnnrrennnrnnrnnnnrnrrennnrnnnnnnnnennenenn 35 Identification Test Operation ececcecceeeeeceeeee seer eeeaeeeeaeeseeeeeeeaeeesaaeeeeeeeseeeesaeseeeeeeeeeess 35 Identification Test Gauges and Indicators rarvrannrnnannvnnrnvnnnnrrnnnnrnnrnnnnrnrrennnrnnnnnrnnenneeenn 37 Chapter 8 Troubleshooting rrssrnnnnvnnnnvnnnnnvnnnnnnnnnvnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnenn 40 Appendix A MIT Products ssvernnannvennnnnnvennnnnnvennnnnnvennnnnnvennnnnnvennnnnnvennnnnnnennnnnnnnnnnnnnnennnnnnnennnnnnenr 42 Appendix B Sample Test Report rrnnnvrnnn
22. T 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Table of Contents Table of Gontents 5asd sendetiden asdendeedee kto bat E E T 3 Introduction aa 4 Purpose of This Guide urnrrnannvnnonvvnrnrrvnnnrnnnn vennen vennnrnnnnnnnnennenrnnrennnnnnsnnnnnnnneennrnennnnnsennenennne 4 AUdENGS uante eee eee 4 ASSUMPUONS vedaene eneren 4 Conventions Used In This Guide rrerannvnnonvvnnnrrnnnnrnnnnnvnnnnvnnnnrrennnrnnnnnnnnenreennrnesnnrnnsnnensnnnen 4 User Guide Organization ruanvrnnnrovnnnrnnnnnrnnenvennnvrnnnnrnnnnnnnnnnrennnrnnennnnnennenrsrresnnrnsennnnnennenenn 4 Customer Support ccccecececeeccececeeeeeaeeeeeeeeeeeeceaeeeeaaesaaeeseaeeeseaeeseaaesdeaeeseaeeesaeseeeaesseneessaees 5 PYOCAUTIONS EE NE EE NE NE EEE 5 ENVIFONIMOMIM EEE RE 5 Chapter 1 Getting Started siisi eesin naea aaan taeao aaaea eraten kand bunken asken 6 Inside the Large BOX vwicccecass cs tepisned erian airaa EEEE EEEE E EAREN S EEEE EEE ENEA 6 Inside the Small BOX rrrrnnnrnnannvnnnnnvnrnrrennnrnnannvnnenrennnrnnannnnnnnnennnrresnnnnnsnnnenenresnnrnsennnnsennenennn 6 1 59 Micron PSL Sphere Suspension Referigeration asrrrrnrrnnnnvrnnnnvnnnrrnnnnrrvnnnrnnrnnnnennn 6 Systemi Set UD EE EE 7 Equipment and Materials Required For an Identification Test rmarrrnnnnrrrrnnnnrnnrnnnnrnnnnn 8 Chapter 2 Quick Reference Identification TeSt rrrnmnnnnnvnnnnvrnnnvvnnnnvnnnnnvnnnnnnnnnvnnnnnvnnnnnnnnnnnnnnnnennn 9 Chapter 3 MIT
23. Test Pass Figure 18 Pass Water Test e If the water quality is not adequate to conduct an Identification Test the Water Test will self terminate as soon as that judgment is determined and will display Water Test Fail refer to Figure 19 Micro Identification Power echnologies MIT Cloud MIT 1000 Ready Active Identifiers Listeria spp When the Program reports Water Test Fail The MIT Vial MUST NOT be used for an Identification test Rinse and refill the vial and try again Troubleshooting Configure Water Test Fail Figure 19 Fail Water Test MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Chapter 6 Sample Preparation Prior to preparing a sample for MIT 1000 Identification Testing it is imperative that prior microbiological functions were performed to create a single species incubated in MIT approved solid medium Step 1 Fill the MIT Vial with Water Items Needed 2 x 2 square of Parafilm 15 mL of LPF water 10 Rubber band 1 Using LPF water e MIT Vial e e e Procedure a b C d Start rinsing the MIT Vial by holding the vial upside down in the water stream Slowly rotate the vial in the LPF water stream to the vertical position Fill the vial with LPF Water Quickly turn the vial upside down and VIGOROUSLUY SHAKE the water out of the vial This should be quick enough that it requires three shakes to empty the vial
24. Vial Parafilm cover 3 Remove or gently lift one side of the Parafilm 4 Pipette 1 ul of the suspension into the MIT Vial as shown in Figure 26 5 Properly dispose of the used tip into a biohazard Figure 26 Inoculating the Colony container Dilution into the MIT Vial 6 Immediately re cover the MIT Vial in the same manner as done in Step 1 on page 29 and shown in Figure 21 Note To ensure optimum performance it is recommended that Steps 8 and 9 are completed within 1 hour of completing step 7 Step 8 Clean the MIT Vial Outside Surface Items Needed e MIT Vial e Kimwipes e 70 IPA Procedure 1 Dampen a Kimwipe with 70 IPA 2 Wipe the entire exterior of the spherical part of the MIT Vial 3 Immediately dry the MIT Vial with a clean dry Kimwipe MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Step 9 Place the MIT Vial into the MIT 1000 System Items Needed e MIT Vial Procedure 1 Start the MIT 1000 Application 2 Liftthe lid 3 Place the MIT Vial in the MIT 1000 System in an upright position as shown in Figure 27 4 Rotate the MIT Vial and stop at a point where laser glare on the MIT Vial is minimal See Figure 28 and Figure 29 for unacceptable and acceptable glare 5 Replace the lid 6 Proceed to Chapter 9 to conduct an Identification Test Figure 27 Placing the MIT Vial into the System Figure 28 MIT Vial Figure 29 MIT Vial Incorrectly Orien
25. ak the collection of contaminated rubber bands on the 5 bleach soaked Kimwipe by spraying them with more 5 bleach Note Allow the rubber bands to sit untouched for at least 20 minutes 7 The rubber bands may be reused after at least a 20 soaking time 8 Autoclave the contaminated MIT Vials at 121 C for 45 minutes 9 Carefully remove the MIT Vial Holder after the sterilization cycle 10 Allow the sterilized vials to cool down to room temperature 37 C approximately 2 hours cool time 11 Discard the sterile solution in each vial 12 Rinse the vial with LPF water 13 Proceed to the MIT Vial Cleaning Procedure MIT Vial Cleaning Items Needed e Autoclave e Sterile MIT Vial e MIT Vial Holder e LPF water e Dish Soap e Distilled White Vinegar solution of about 5 to 8 acetic acid in water and a pH of about 2 4 2 2000 mL beakers or equivalent MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Procedure 1 In one beaker make a soapy solution by filling it with 2 liters of lukewarm water and ONE drop of dish soap 2 Stir the soap drop into solution without making excessive bubbles 3 In the second beaker make at least one liter of a 10 Vinegar solution of LPF water and distilled white vinegar Example 900 mL of LPF water 100 mL of Vinegar 4 Stir the LPF Water and Vinegar solution to mix 5 Hold the MIT Vial firmly and fully submerge the MIT Vial in the soapy water so
26. ar counts down the test Micro Iden tification echnologies MIT 1000 Active Identifiers Check Vial Listeria spp T Cloud Ready Concentration The Check Vial monitor will flash red when the vial must be repositioned The SCG shows particle concentration and should re remain in the left red zone Configure Busy B MIT Web Click Stop Water Test to no prematurely quit the water quality test The Busy indicator flashes green during the test Figure 17 Water Testing In Operation MIT 1000 System User Guide V3 5 Micro Joenr IFICATION ECHNOLOGIES Note If the Check Vial indicator blinks during the Water Test the MIT 1000 Application will terminate the test This means that there is a scratch on the MIT Vial in the path of the laser beam The easiest corrective action is to rotate the flask until the glare is eliminated see Figure 28 amp Figure 29 and then restart the Test Water Test Results e If the water quality is good the test will require 30 seconds to execute and the MIT 1000 Application will display Water Test Pass refer to Figure 18 viT1000RMID EE son Micro Identification Power echnologies MIT Cloud MIT 1000 Ready Identifiers Active Identifiers borr When the Program reports Water Test Pass the Sample Vial may be used for an Identification test Troubleshooting Configure Water
27. ct No PO No caption e Select the Project and PO Numbers associated with the test All Task and Lot Numbers associated with this Project and PO Number will be listed below the Task No Lot No caption e Select the Task and Lot Numbers associated with the test All Samples measured associated with the Task and Lot Numbers will be listed below the Sample Name caption e Select the Sample Name associated with the test After each selection all tests performed for the selected Sample job information will be listed Test Report The MIT 1000 Application can create a test report for each test in the log as shown in Figure 6 To display the report find the test in the Sample Test Log as described above Then either e Click on the test entry and click on Show Report at the bottom of the page The Show Report button is visible only when a test is selected OR e Double click on the test entry The Test Report Form appears Figure 6 Test Report MIT Foods System 000020 Laboratory Report Printed 10 23 Customer MIT Foods Project Number Burrito PO Number 12 MITF Task Number 002 Burrito Lot Number 2 Sample Identifier Cooking 2 Test 000211 Identification Result Listeria spp The report header is determined by the text shown in the Header Text box To change the header text enter the new header in the Header Text box then click Comments Apply Selected Identifiers
28. entifiers Visit the MIT web site for available Identifiers Light Scattering The phenomena that occurs when a particle passes through the laser beam inside the MIT 1000 System LPF Water Low Particle Filtered Water Typically this is water that is the product of a water filtering system with a final filter of 0 1 micrometers absolute or smaller The MIT 1000 Application Water Test function is the final decider on whether or not water can be used for Identification tests with the MIT 1000 System Material required The Material needed to perform an Identification Test that is NOT supplied by MIT MIT website www micro identification com mL Milliliter Notes Useful information or information that requires special emphasis Power Services The AC electrical power that is typical for your country of usage PSL Polystyrene Latex spheres nl 1000 The program used to control the MIT 1000 System Application MIT 1000 System MIT 1000 Rapid Microbial Identification System MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES MIT Vial The vessel used to prepare a sample and conduct an Identification Test MIT Part Number 10007 MIT Vial Holder A device to hold the MIT Vials Sample Test Log The Log used to store pertinent test information SCG Sample Concentration Gauge The meter used to provide a visual assessment of the quality of t
29. he Sample made by the User The Sample is considered acceptable if the pointer in the gauge is consistently in the Green area Setting up the The schematic to connect all of the sub systems of the MIT 1000 System System Soapy Water Approximate ratio of 1 drop dish detergent Dawn Joy etc to 2 liters of Solution water Starter Kit Materials needed to for first operation of the MIT 1000 System The flap on top of the MIT 1000 System cover used for placing and removing Lid MIT Vials Warni Information to help avoid injuring the User damaging the equipment or arning reducing the System s performance Water Test The test performed to verify that the water being used in the Sample to be tested has no more than 3 particles after a 5 minute test MIT 1000 System User Guide V3 5 Configuration Culture Medium Customer General Functions Medium Project Sample Task UseTor Control Display Button Functions License Culture usage Customer Support Equipment Required Gauges Sample Concentration Identification Test Gauges and Indicators Operation Performing Quick Reference Indicators Check Vial High Concentration Low Concentration Test Progress License Additional License Purchase Logs and Reports Lab Test Recorded Log Information Sample Test Log Test Report Micro IDENTIFICATION ECHNOLOGIES Index 17 Test Report Header 19 Material Required 20 Micro Centrifuge Tube Suspension 18 MIT 1000
30. k the Down Arrow to add the highlighted Licensed Identifier to the Active Identifier list Active Identifiers E coli Listeria spp Figure 4 License Display Click the Up Arrow to remove the highlighted Licensed Identifier from the Active Identifier list Only those Identifiers listed here will be used during an Identification test To use a specific Identifier during an Identification test simply double click the desired Identifier Arrow This moves the Identifier genus associated with all selected Identifiers M in the Licensed Identifiers list or highlight the Identifier and then click the Down to the Active Identifiers list The Active Identifiers list can contain more than one Identifier The MIT 1000 System will test for the presence of species and during a single Identification test In the example in Figure 4 the E coli and Listeria spp Identifiers are selected for an Identification Test To remove an ldentifier from the Active Identifiers list simply double click it or select it by clicking on it and then click the Up f Arrow For specific installation operation and precautionary statements please refer to the Insert that accompanied the Identifier MIT 1000 System User Guide V3 5 M ICRO IDENTIFICATION ECHNOLOGIES To purchase additional licenses contact your local representative or Customer Service Log
31. l to close the form without accepting the new Sample Edit Click Edit right of the New button to modify the selected Sample When the Sample Form Figure 13 appears the Sample Name and Notes fields will be filled in with the selected Sample s information Modify the fields as needed and click OK to accept the changes or click Cancel to close the form without accepting the changes Table 8 Sample Configuration Options MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Upon clicking the New or Edit button associated with Sample the Form shown in Figure 13 will open To add a new or edit the Sample Enter the new Sample Name Enter any notes or comments Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 13 Sample Entry Information Show in the Lab Test Report The Lab Test Report always shows the name of the Sample tested In addition the User has the option of displaying the Customer Name Project Name PO Number Task Number and Lot Number on the Lab Test Report simply by checking the appropriate box in the Configure Form as shown in Figure 14 Show in Report v Customer Name V Project Number 8 I Configure S 1 3 WW PO Number MIT 1000 S N 000020 Medium VI Task Number F Chime on completion TSA ar KE TSA wi5 SB V Lot Number m Require reason for U
32. linking the sample concentration is Concentration too high Active Identifiers Check Vial E coli Identifiers Listeria spp Salmonella spp The Sample Concentration Gauge indicates the sample concentration is high Troubleshooting Configure MIT Web Stop Identify Figure 34 High Concentration Display MIT 1000 System User Guide V3 5 Miczo Joenr IFICATION ECHNOLOGIES Check Vial indicator When the Check Vial indicator begins blinking red Figure 35 MIT Vial was positioned in the System such that a vial surface scratch or defect is in the path of the laser beam This causes excessive glare and prevents the System from completing an Identification Test The Check Vial indicator will blink red and the SCG will point to the far left red zone Figure 35 After five seconds the MIT 1000 Application will terminate the Identification Test and display the text Terminated vial glare in blue Figure 36 To correct this reposition the MIT Vial If the condition persists a different MIT Vial must be used Power Micro Identification When the Check Vial echnologies MIT Cloud light is red and blinking the MIT 1000 system MIT 1000 Ready laser is illuminating a scratch or defect on the m Concentration Sample Vial surface Check Vial The vial must be repositioned to eliminate the excessive glare User Guide
33. m will appear The User must enter the required information before the Identification Test can start Clicking the Water Test button begins the Water Quality test Water that does not pass this test MUST NOT be used for Identification Tests Clicking the Close Program button will terminate the MIT 1000 Application 5 The MIT 1000 Application MUST be connected to the MIT Cloud to operate MIT 1000 System User Guide V3 5 List of Licensed Identifiers TM Micro IDENTIFICATION ECHNOLOGIES MIT 1000 Application Display Button Functions License Upon clicking the Identifers button Figure 2 the Identifiers Form shown in Figure 4 will appear The upper list under the Licensed Identifiers caption lists all the Identifiers for which you have a current license to use The MIT 1000 System cannot be used to identify a specific species or genus unless the license for the Identifier for that species or genus is current Visit the MIT website for a complete list of available Identifiers The MIT 1000 System will identify only those bacteria that are in the bottom list below the Active Identifiers caption The User must select the Identifiers that are to be active for Identification tests In the example shown in Figure 4 the System has current license to the following Identifiers e E coli e Listeria spp e Salmonella spp Licensed Identifiers E coli Listeria spp Salmonella spp Clic
34. mperature Task Number Now Edit Naw Edit I Incubation Time I Lot Number Sample Tracking Customer Project Task Sample Customer MIT Project Software Deverlopment PO Notes Task Testing Lot Number tt Notes New _ Edit New Edit 1 New Edit New Edit Sample Tracking Sample Tracking Parameters Parameter Summary Figure 7 Configure Form MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES General Function Descriptions MIT 1000 S N This is the serial number of the MIT 1000 System This MUST match the six digit serial number located on the back of the System The MIT 1000 Application automatically fills in this value Chime on Completion Checking this option causes the System to sound a chime when the Identification Test or Water Test is complete User Termination Reason Checking this option will require that the User specify a reason for terminating an Identification Test Required Media Checking this option will require that the User specify the culture Log media used to grow the bacteria when an Identification Test is Entries performed Media Lot Checking this option will require that the User specify the lot Number number of the media used to grow the bacteria when an Identification Test is performed Media Checking this option will require that the User specify the Expiration expiration date of the media used to grow the bacteria being tes
35. opriately Environment This System operates on various power services 110 220 VAC 50 60 Hz 15 10 amps requires no reagents or chemicals has a small footprint of 1 25 cubic feet and weighs less than 25 pounds The software included in the System operates on a Windows 7 platform in a personal computer MIT recommends that the MIT 1000 be placed in an environment that does not exceed the following parameters Environmental Temperature Relative Humidity Altitude Vibration Factor Range Operating 41 F to 95 F 20 to 90 Sea level to Vibration sensitive 5 C to 35 C non condensing 10 000 ft 3 km Do not operate near fans or motors Normal keyboard and mouse activities are OK Non Operating 4F to 140 F 95 Sea level to 20 to 60 non condensing 10 000 ft 3 km The System uses MIT s proprietary MIT 1000 Application software and Identifiers to identify organisms Updates of the MIT 1000 Application software and a list of available Identifiers is available on the MIT web site www micro identification com MIT offers all accessories i e additional test vials culturing media pipettes and other lab supplies necessary to operate the System Should you wish to purchase any of these items please contact your sales representative or contact us directly via email at service micro identfication com or telephone us at 949 388 4546 MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHN
36. pecies not identified by one of the Active Identifiers the MIT 1000 Application will display Test Result Unknown rare MIT 1000 RMID Micro Identification why Power O echnologies S N MIT Cloud MIT 1000 Ready Active Identifiers E coli Listeria spp Salmonella spp Troubleshooting Identification test result o Sonne Test Result Listeria spp Figure 32 Identification Completion Display MIT 1000 System User Guide V3 5 Micro IDENTIFICATION p ECHNOLOGIES Identification Test Gauges and Indicators Identification Test Progress Indicator This indicator provides the User with a coarse estimate of the test completion progress Once the progress has reached the halfway mark completion may occur abruptly Typically the test process takes 2 minutes but occasionally may complete as quickly as 30 seconds or as long as 5 minutes Note The Identification Test will terminate automatically after 5 minutes and display in blue Terminated time out Sample Concentration Gauge SCG During the identification process the MIT 1000 Application estimates the concentration of the sample being tested The Sample Concentration Gauge SCG gives a real time indication of this estimate The gauge pointer will point in the middle of the green zone when the sample is properly made Concentration Indicator Low Concentration Indication When the sample concentration is too low
37. s the Task Number Lot Number and Notes fields will be filled in with the selected Task s information Modify the fields as needed and click OK to accept the changes or click Cancel to close the form without accepting the changes Table 7 Task Configuration Options MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Upon clicking the New or Edit button associated with Task the Form shown in Figure 12 will open z Task Task Number Lot Number Notes To add a new or edit the Task 1 Enter the new Task Number and or 2 Enter a Lot Number 3 Enter any notes or comments 4 Click OK to accept the new entry or click Cancel to close the dialog without accepting the new entry Figure 12 Task Entry Information Sample Configuration To input a Sample Name and other related information follow the instructions in Table 8 Sample List box Click the Down Arrow to show the list of Samples that have already been entered for the selected Customer Project and Task The parameters for the selected Customer Project Task and Sample are shown in the lower left of the form New Click New right of the Sample List box to add a new Sample for the selected Customer s Task to the list When the Sample Form Figure 13 appears fill in the Sample Name and enter any comments or notes on the Sample Then click OK to accept the new Sample or click Cance
38. s 1 3 do not correct the problem e MIT 1000 Application is contact MIT Customer Service corrupted Identifier list does not match e Possible MIT Cloud 1 Consult MIT Customer Service with what was ordered configuration error System does not Identify or e Power is off 1 Verify the System is powered on frequently does not recognize any e Water particulate 2 Runa Water Quality Test bacteria examinan concentration is too 3 Verify the lid is seated properly on the high System e MIT Vial is dirty 4 Verify the laser is operating look for red e Lid is not seated glow with Lid up properly 5 If none of the above solves the problem e Laser is not operating contact MIT Customer Service Check Vial light blinks red or e The laser beam is 1 Stop the Identification or Water Test The Terminated vial glare is illuminating a scratch or test will automatically terminate after 5 displayed other defect on the seconds The Identification or Water Test me i 2 Rotate the MIT Vial until the red glare will terminate when this condition causing It to glow re seen in the vial neck is not visible persists for more than 10 seconds The water in the MIT 3 If 2 does not solve the problem replace and displays Vial contains too many the MIT Vial with a new one Terminated vial glare particulates 4 Restart the Identification or Water Test 5 If none of the above solves the problem contact MIT Customer Service MIT 1000 S
39. s and Reports Sample Test Log The MIT 1000 Application records all Identification Test activities Click Log in the MIT 1000 Application Display to view the Sample Test Log Figure 5 Maximizing the window enables the User to view more rows and columns of the Log Figure 6 below shows a partial view of the Sample Test Report that can be viewed and printed for each entry in the Sample Test Log Test Number Select a date to show all tests performed on that date Select Sample specific information to show a log of tests Log Yekr Date Customer Project No PO No Task No Lot No Sample Name User 2011 2012 07 13 11 MITF Burrito 001 Burrito 1 Cooking 1 _ 2002 2012 07 16 Customer A 12 MITF Tamale 2012 07 27 BDFE Meats Cooking 3 G Kessler 2012 08 29 12 MITF Monitor Chilling 1 C Frank 2012 08 31 Chilling 2 S Pfeiffer 2012 10 16 Chilling 3 2012 10 17 PrepBegin 1 2012 10 18 PrepBegin 2 2012 10 19 PrepBegin 3 Test Time Sample Result Gram Stain Customer Name Project Number PO Number 000125 2012 08 31 09 40 20 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000145 2012 09 03 09 50 24 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000168 2012 09 04 09 52 33 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 0001 78 2012 09 05 09 23 14 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 000194 2012 09 06 09 45 54 Cooking 2 Unknown Not done MIT Foods Burrito 12 MITF 00020
40. select or type are shown in bold User Guide Organization This Guide is organized as follows Introduction Describes the major features and benefits of the MIT 1000 System Chapter 1 Getting Started provides a list of components and materials included with the MIT 1000 System and those standard laboratory equipment and materials needed to complete an Identification Test Chapter 2 Quick Reference Operation Summary provides a quick reference guide to operate the System Chapter 3 Describes the features of the MIT 1000 Application Chapter 4 MIT Vial Cleaning and Storage Chapter 5 Water Quality Test Chapter6 Sample Preparation Chapter 7 Performing an Identification Test Chapter 8 Troubleshooting Appendix A MIT Products Appendix B Sample Test Report Glossary Index MIT 1000 System User Guide V3 5 Micro DENTIFICATION ECHNOLOGIES Customer Support If the System appears to be operating incorrectly or you have specific operational questions please contact Customer Service at service micro identification com or call us at 949 388 4546 Precautions The MIT 1000 System is a universal system designed to identify various bacteria many of which are pathogenic Take extreme care in handling all specimens microbial cultures and inoculated MIT Vials Aseptic technique and usual precautions for handling the bacteria group should be observed throughout the Identification Test process Ensure all biohazardous waste is disposed of appr
41. ser Termination Strip chart Required Log Entries Media Show in Report V Customer Name Signal Noise kample Tracking E Media Lot Numbe VI Project Number E Media Expiration O te V PO Number A Incubation Temperature V Task Number eee a 3 New Edit New Edit collection _ Incubation Time IV Lot Number Customer Project Sample A Sample Peren Project Software Deverlopment PO Notes Task Testing mu 7 Lot Number 4 Notes New Edit New Edit New Edit New Edit Figure 14 Configure Form Show in Lab Report MIT 1000 System User Guide V3 5 Micro IDENTIFICATION i ECHNOLOGIES Chapter 4 MIT Vial Cleaning and Storage Sterilizing the MIT Vial Items Needed Autoclave Contaminated MIT Vial Spray bottle with 5 bleach Kimwipe Biohazard Bin with Biohazard Bag MIT Vial Holder Figure 15 MIT Vial Holder Procedure 1 Place a Kimwipe on the tabletop 2 Soak the Kimwipe with 5 bleach 3 Remove the Parafilm and rubber band a Deposit the contaminated Parafilm into the Biohazard Bag b Place the rubber band from the contaminated MIT Vial onto the 5 bleached soaked Kimwipe 4 Carefully place the contaminated vial on the MIT Vial Holder 5 Place the MIT Vial Holder s with vials into the autoclave 6 After all autoclaved vials have been processed so
42. signed to the purchase order or work order PO Number The Purchase Order number for the work being performed Task Number The Task Identifier in the Project Lot Number The Lot Number of the item s being tested for the selected Task Identifiers NUT ee The Identifiers that were used during the Identification Test Application The User that performed the Identification Test The culture media used for bacteria growth Media Lot The lot number of the culture media The expiration date of the culture media Expiration E The temperature used for bacteria growth emp The date and time the incubation period started dr The date and time the incubation process ended on MIT 1000 The reason the Identification Test was terminated e g high Termination NG Table 1 Log Entry Log entry Definition MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Finding a Test in the Sample Test Log The sample Test Log provides two independent methods of finding the tests By date and by Sample By Date Click the Year the test was performed in the Year list in the upper left corner of the form Then click the date the test was performed in the Date list next to the Year list After each year and date selection all tests performed on the selected date will be listed By Sample e First select the client name in the Customer list All Project and PO numbers associated with this client will be listed below the Proje
43. ted Date when an Identification Test is performed Incubation Checking this option will require that the User specify the Temperature temperature the bacteria being tested was incubated Incubation Checking this option will require the User to specify the incubation Time start and stop time and date for the bacteria being tested Table 2 General Function Descriptions Note The Diagnostic Tools are for MIT Technical Representative use only MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES Medium Configuration To configure the Medium follow the instructions in Table 3 Medium List The Medium list box shows the available options that the User can select box prior to starting an Identification Test and the Required Log Entries Media check box is selected The MIT 1000 Application comes preconfigured with two predefined entries BHI Brain Heart Infusion and TSA w 5 SB Trypticase Soy Agar with 5 Sheep blood New Click New to add a new media selection option When the Media Name Form Figure 8 appears fill in a name for the new Media Then click OK to accept the new Media or click Cancel to close the form without accepting the new Media Edit Click Edit to modify the name of the selected media When the Media Name Form Figure 8 appears the current Media Name is filled in Modify the name and click OK to accept the change or click Cancel to close the form without accepting the
44. ted with Correctly Oriented Unacceptable Glare with Acceptable Glare MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Chapter 7 Performing an Identification Test This chapter will describe how to perform Identification Testing using the MIT 1000 Application MIT 1000 Application Start up Start the MIT 1000 Application by clicking the icon Figure 1 Page 10 on the computer desktop Identification Test Sample Information Upon clicking the Identify button the Sample Information Form below will appear As a prerequisite to the Identification Test the User is required to enter the information in Figure 30 Click OK to accept the Sample Information and continue with the Identification Test Click Cancel to abort the test Sample Information Optional Sample Required Sample User G Kessler new Meda Tracking Information Speed Media z Information 1 User Customer MIT Foods L t Number 1 Media 2 Sample source Project 12 MITF Burrito Expiration Date 10 22 2012 parameters information ay J 2 Sample 3 Gram Stain sample Cooking 2 Te New Incubation incubation results Temporaire A parameters EGGE Start Time 12 30 99 1200AM G Notdone C GNR GNC Yeast z 3 Comments C GPR C GPC End Timer 12 30 99 v 12 00AM Comments Figure 30 Identification Test Sample Information Form MIT 1000 System User Guide V3
45. that the vial neck is pointing upward 6 When the MIT Vial is fully filled with soapy water lift the filled vial out quickly turn the vial upside down and VIGOROUSLY SHAKE the content out so the content ejects with three shakes Note Do not return the vial content back into the beaker 7 Repeat step 6 at least five times 8 Rinse and fill the MIT Vial to the top with LPF Water and discard the content with VIGOROUS SHAKING so that the content ejects with three shakes 9 Repeat step 8 at least five times 10 Fully submerge the MIT Vial in the 10 vinegar solution with the neck pointing upward 11 When the MIT Vial is fully filled with the 10 vinegar solution lift the vial with the vinegar content out quickly turn the vial upside down and VIGOROUSLY SHAKE the content out so the content ejects with three shakes Note Do not return the vial content back into the beaker 12 Repeat step 11 at least five times 13 Rinse and fill the MIT Vial to the top with LPF Water and discard the content with VIGOROUS SHAKING so that the content ejects with three shakes 14 Repeat step 13 at least five times 15 Place the MIT Vial upside down in an MIT Vial Holder to air dry Storing MIT Vials When stored in a clean room that has HEPA filters in continuous operation the MIT Vials can be stored on an MIT Vial Holder When the MIT Vials are stored in other dirtier environments cover the vial opening with a 2 x 2 square of Parafilm and
46. tware Upgrades Calibration Identifiers Supplies Plated media Micro centrifuge tubes Disposable loop Vial Caps Water replacement filters Pipette tips MIT Vials Pipette Volume Device Parafilm Power Supply Module Equipment MIT Computer Pipette Vortex Mixer MIT Vial Holder MIT Water filtration System M ICRO IDENTIFICATION ECHNOLOGIES Appendix A MIT Products Description MIT 1000 system Power supply module MIT Mini Notebook w MIT 1000 Application Starter Kit Listeria spp Identifier Micro centrifuge tubes Sterile loops Sample Vials Pipette tips Parafilm 2 X 2 squares MIT Vial Holder 6 9 or 12 month E coli Listeria spp Salmonella spp Trypticase Soy Agar TSA with 5 Sheep Blood 15 x 100 mm plates pack of 10 1 5 uL Micro centrifuge tubes Locking lid pack of 500 1 uL Loop 96 Packs of 10 3 Stage 10 uL Aerosol barrier 10 racks of 96 15 mL MIT 200 2 X 250 ft roll MIT 1000 power supply Mini Notebook with MIT 1000 Application 0 5 to 10 uL variable Fixed Speed 6 Vail holder TBD Wall mounted filtration system periodically Catalog 1000 1001 1002 1003 1004 10000 10001 10002 20001 20002 20003 20004 20005 20006 20007 20008 20009 20010 30000 30001 30002 30003 30004 MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Appendix B Sample Test Report MIT Foods Customer MIT Foods Project N
47. umber Burrito PO Number 12 MITF Task Number 002 Burrito Lot Number 2 Sample Identifier Cooking 2 Test 000211 Identification Result Listeria spp Test Time 2012 10 23 09 40 37 User G Kessler Termination Normal Not performed User Approval Date MIT 1000 RMID version 1 0 Page 1 of 1 MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Glossary pL Microliter Basic Laboratory Basic microbiology procedures that include microbial handling culturing Procedures disposing Calibration The method that uses calibrated polystyrene spheres to determine if the MIT 1000 is working properly Equipment The Equipment needed to perform an Identification Test that is NOT required supplied by MIT ID Identification Test IPA Isopropyl Alcohol Identification Result The name of the bacteria sphere or Unknown organism determined by an Identification Test Identifier An MIT proprietary set of data that is used by the MIT 1000 System to identify the Genus Species or Subspecies of a bacterial specimen Identification Test The test performed by the MIT 1000 System to determine if the microbial sample being tested contains the bacteria or particle associated with the selected Identifiers Inside the Box The MIT 1000 System list of equipment and materials that was included in your purchase order License Provides access to Id
48. ure button to configure the MIT 1000 Application Click the MIT Web button to display the Micro Identification Technologies web page www micro identification com MIT 1000 System User Guide V3 5 M ICRO DENTIFICATION ECHNOLOGIES System power indicator Cloud connection indicator System ready indicator Power MIT Cloud Ready MIT 1000 List of active identifiers Start a Water Quality Test button Active Identifiers Listeria spp Close the RMID application button Troubleshooting Start an Identification Test button Configure MIT Web Close Program Identify Figure 3 MIT Central Control Display The System operation Figure 3 status is shown on the right side of the Display The Power indicator is green when the MIT 1000 System is powered on and shows no color when the power is off The MIT Cloud indicator is green when the MIT 1000 Application is connected to the MIT 1000 Cloud and flashes red when it is not connected The Ready indicator is green when the MIT 1000 Application is properly configured and the MIT 1000 System is connected and operating properly It blinks red when a configuration or connection error occurs The Active Identifiers pane in the middle of the Display shows the Identifier that will be used during an Identification Test Clicking the Identify button begins the identification process Note once the Identify button is clicked the Sample Information for
49. vnnnnnvnnnnnnnnnvnnnnnvnnnnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnenn 43 eor LA A AE PE A A EAA AE E E A E A E TE 44 MON OX SEE E T 46 MIT 1000 System User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Introduction Congratulations on purchasing the revolutionary MIT 1000 System This instrument uses the principles of light scattering to identify a variety of bacteria in less than five minutes Purpose of This Guide This Guide describes how to prepare operate and troubleshoot the MIT 1000 Rapid Microbial Identification System Audience This Guide is for users technicians microbiologists scientists and researchers of all skill levels who will use and maintain the MIT 1000 System Assumptions This Guide assumes the User is familiar with e Basic Microsoft Windows operations such as using a mouse choosing and selecting commands working with Windows e Basic computer set up skills that include connecting power module and USB cable to the appropriate ports on the computer e Basic laboratory techniques and has a basic microbiological background Conventions Used In This Guide This Guide uses the following conventions e Notes are underlined and provide useful information or information that requires special emphasis e Warnings are in yellow and provide information to help avoid injuries to the User damaging the equipment or reducing the System s performance e When using the MIT 1000 Application items that you
50. ystem User Guide V3 5 Micro IDENTIFICATION ECHNOLOGIES Condition Probable Cause Recommended Action Concentration light blinks red or e Excessive Discard the Sample and make a new one Terminated high concentration concentration bacteria Re test is displayed or foreign particles in If this does not solve the problem contact The Identification Test will the MIT Vial MIT Customer Service terminate when this condition persists for more than 10 seconds and display Terminated high concentration Concentration light blinks blue or Too low concentration Discard the Sample and make a new one Terminated low concentration of bacteria in the MIT Re test Vial If this does not solve the problem contact The Identification Test will MIT Customer Service automatically terminate when this condition persists for more than 10 seconds and display Terminated low concentration Terminated time out The sample was Discard the Sample and make a new one contaminated with Re test particles agar more If this does not solve the problem contact than one species of MIT Customer Service bacteria dirt etc that prevented identification The Identification Test will automatically terminate an Identification Test if there is no result after 5 minutes and display Terminated time out MIT 1000 System User Guide V3 5 Item MIT 1000 System Starter Kit Yearly Maintenance Sof
Download Pdf Manuals
Related Search