Users Guide - Bascom Palmer Eye Institute
Contents
1. 3 Fluorescence scanning Sl EXCMO aieia ea 5 2 EMISSIOHTIItErS stt e RR 3 3 About the Fluorescence Setup window s 3 3 1 Displaying the Fluorescence Setup window 3 3 2 Selecting the emission filter 3 3 3 Selecting the laser 4 Troubleshooting ME elo d 23 5 Technical Specifications 57 Regulatory requirements a 25 5 2 Technicald ta suere t n ERI RUM ERR procasa nace 25 Typhoon Trio Users Guide Addendum 00 4278 46 Edition AB 5 Contents 6 Typhoon Trio Users Guide Addendum 00 4278 46 Edition AB Preface Preface About this addendum The Typhoon Trio User s Guide Addendum v 5 0 provides an overview of the Typhoon Trio and the Typhoon Trio model instruments This addendum describes only the features and functions that differ from the Typhoon instrument models described in the Typhoon User s Guide v 3 0 You should use this addendum in conjunction with the user s guide to learn how to operate and maintain the Typhoon Trio or the Typhoon Trio system Related publications In addition to the Typhoon Trio User s Guide Addendum Typhoon User s Guide and Typhoon Installation Instructions you should be familiar with the ImageQuant TL image analysis software Safety Chapter 2 in this addendum and chapter 2 in the Typhoon User s Guide provide important safety information that should be used when operating the Typhoon system Before using the Typhoon system read and understand the safety2. cause undesired operation 5 2 Technical data Operating temperature 4 C to 40 C Relative humidity Less than 80 from 4 C to 31 C decreasing linearly to 50 from 31 to 40 C Voltage 100 240 V Max Current 6 3 A Frequency 50 60Hz Dimensions h x w x d 480 x 300 x 780 mm Weight maximum 160 kg 350 Ibs Fuse T6 3 AL 250V Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 25 Technical Specifications 5 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 26 For contact information for your local office please visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com imagination at work Cy DeCyder Deep Purple ECF ECL Plus ImageQuant and Typhoon are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company All third party trademarks are the property of their respective owners All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information 2004 2008 General Electric Company All rights reserved First published September 2004 GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Europe GmbH Munzinger Strasse 5 D 79
3. description of how to use the multislide tray 13 Scan acquisition modes You can scan samples on the Typhoon Trio or Typhoon Trio instrument using the following scan acquisition modes e Storage phosphor mode Creates images from samples labelled with radioisotopes using storage phosphor technology e Fluorescence mode Creates images from samples labelled with fluorescent dyes For details on the available excitation modes see chapter 3 e Chemiluminescence mode Creates images from chemiluminescent samples 14 Before using the system Before using the Typhoon Trio system you should become familiar with e Safety information It is imperative that you follow the precautions in this addendum and in the Typhoon User s Guide chapter 2 e Basic operational instructions See the Typhoon User s Guide e Maintenance information for the instrument and accessories See the Typhoon User s Guide Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 15 1 Introduction 1 4 Before using the system 16 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 2 Safety Safety 2 WARNING The operator of the Typhoon system is assumed to be trained in the correct operation of the system and the safety issues Throughout the Typhoon User s Guide and this addendum the word you refers to this trained operator Using controls making adjustments or performing procedures other than those specified in the Typhoon User
4. high background interference For the best results you should test the membrane samples with different laser and emission filter combinations to determine the optimal combination 20 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Fluorescence scanning 3 3 3 3 Selecting the laser When you select a filter Scanner Control automatically selects a default laser to use with the emission filter you selected However if desired you can select a laser other than the default If you select a laser other than the default and then Click OK a message appears and asks you to confirm the selection The Typhoon Trio laser choices are e Green 532 e Red 633 e Blue 488 e ECL4 Excitation The laser power varies from one wavelength to another You might need to adjust the PMT voltage accordingly For a description of the laser excitation modes see section 3 1 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 21 3 Fluorescence scanning 3 3 About the Fluorescence Setup window 22 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Troubleshooting 4 4 Troubleshooting For basic troubleshooting guidelines see the troubleshooting section of the Typhoon User s Guide In addition to the guidelines presented in the Typhoon User s Guide the following error message appears if the Typhoon Trio instrument has a problem communicating with the internal blue laser Failed Communication with Internal Laser Unit Ift
5. information thoroughly Special safety text Make sure you follow the precautionary statements presented in this guide WARNING Indicates a possibility of severe or fatal injury to the user or other persons if the precautions or instructions are not observed CAUTION Indicates that damage to the instrument loss of data or invalid data could occur if the user fails to comply with the advice given IMPORTANT Highlights information that is critical for optimal performance of the system Note Identifies items of general interest Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 7 Preface Trained operator WARNING The operator of the Typhoon system is assumed to be trained in the correct operation of the system and the safety issues Throughout the Typhoon Trio User s Guide Addendum and the Typhoon User s Guide the word you refers to this trained operator Assumptions The software related instructions in this addendum assume you have basic computer skills You should be familiar with the Microsoft Windows graphical user interface If you do not have these skills refer to the documentation or the Help for Windows Safety standards Compliance with standards The declaration of conformity is valid for the instrument only if it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except for alterations described in the User Manual e connected to ot
6. s Guide or this addendum can result in hazardous exposure to laser light high voltage or moving parts Exposure to these hazards can cause severe injury or death 21 Safety overview Make sure you understand and follow the safety precautions in the Typhoon User s Guide including e General safety precautions e Typhoon serial numbers and safety labels e Electrical safety e Pinching and general warnings e Hazardous materials precautions See section 2 2 of this addendum for laser light safety 22 Laser light safety The Typhoon Trio instrument is a Class laser instrument that houses three Class IIIB lasers blue green and red Under the specified operating procedures the instrument does not allow operator exposure to laser light However the lasers are accessible in the interior of the instrument The blue laser has a specified maximum output of 27 mW at 488 nm in a diverging beam The green laser has a specified maximum output of 25 mW at 532 nm in a diverging beam The red laser has a specified maximum output of 13 mW at 633 nm in a diverging beam The safety interlocks in the Typhoon instrument are designed to prevent you from being exposed to the laser beams If you open the sample lid while the scanner is in operation the laser shutter closes and blocks the light from the lasers Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 15 2 Safety 2 2 Laser light safety AN WARNING Do not attempt
7. to defeat the safety interlocks on the sample lid or try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury For example viewing the laser light directly can cause blindness The labels shown in figure 2 1 are affixed to the instrument and warn of exposure to laser light CLASS 1 LASER PRODUCT Per IEC 60825 CAUTION Laser Radiation when open AVOID EXPOSURE TO BEAM International compliance Eye exposure Direct exposure Fig 2 1 Laser light warning labels 16 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 3 Fluorescence scanning 3 Fluorescence scanning This chapter describes the emission filters and the fluorescence setup for the Typhoon Trio and Typhoon Trio instruments The topics are e Excitation modes section 3 1 e Emission filters section 3 2 e About the Fluorescence Setup window section 3 3 See the Typhoon User s Guide for details on the following e About fluorescence scanning including how fluorescence works beamsplitters linking fluorescent scans multilabel experiments and common fluorochromes e Setting up for fluorescence scanning including preparing and placing the sample in the instrument e Scanning a fluorescent sample including checking the instrument state selecting the scan parameters selecting the fluorescence parameters removing the sample from the instrument cleaning th
8. 111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan User Manual 00 4278 46 AB 06 2008
9. GE Healthcare Typhoon Trio User s Guide Addendum Version 5 0 for Microsoft Windows Important User Information All users must read this entire manual to fully understand the safe use of Typhoono Trio IMPORTANT Typhoon Trio is intended for laboratory use only not for clinical or in vitro use or for diagnostic pur poses WARNING The WARNING sign highlights instruc tions that must be followed to avoid per sonal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The CAUTION sign highlights instructions that must be followed to avoid damage to the product or other equip ment It is important not to proceed until all stated condi tions are met and clearly understood IMPORTANT The IMPORTANT sign highlights information that is critical for optimal performance of the system Note The Note sign is used to indicate information important for trouble free and optimal use of the product Recycling This symbol indicates that the waste of elec trical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufac turer for information concerning the decom missioning of your equipment WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take ad
10. ard set of emission filters and can accommodate a total of 13 emission filters The instrument uses emission filters to reject reflected and scattered excitation light and background fluorescence while allowing the emitted light from the sample to pass through to the PMT Table 3 1 lists the standard set of emission filters used with fluorescence scanning Table 3 1 Standard set of emission filters for the Typhoon Trio and Typhoon Trio instruments Emission filter Description 520 nm band pass filter 520 BP 40 555 nm band pass filter 555 BP 20 580 nm band pass filter 580 BP 30 610 nm band pass filter 610 BP 30 670 nm band pass filter 670 BP 30 526 nm short pass filter 526 SP 390 nm band pass filter 390 BP 100 Transmits light between 500 nm and 540 nm and has a transmission peak centered a 520 nm Use this filter with fluorochromes such as Cy 2 ECL Plus and fluorescein Transmits light between 545 nm and 565 nm and has a transmission peak centered a 555 nm Use this filter with fluorochromes such as R6G and HEX Transmits centered a ight between 565 nm and 595 nm and has a transmission peak 580 nm Use this filter with fluorochromes such as TAMRATM and Cy3 Transmits light between 595 nm and 625 nm and has a transmission peak centered a 610 nm Use this filter with fluorochromes such as Deep Purple total prote
11. ce and then click Setup The Fluorescence Setup window f A ition Mod appears figure 3 2 Ce ee Typhoon Scanner Control c data temp scan file ds x File Templates Tray Help Scanner Information r Setup Model Typhoon Trio Address 127 0 0 1 Serial Number 11772944 User Name 1 r Acquisition Mode 34 Instrument State l I Busy E ee 0 m bse Setup Tray Template default R us Sore hd fi ba a 4 Phosphor Mode ES Ses A e Best Sensitivity Best Resolution N r Options M L Orientation ex Press Sample K J Pixel size Focal Plane l 200 microns z Platen H d User Comment E a D E El B A Image Analysis None Aj 1234567 8 97101112131415 1617 181920212 I DIGE File Naming Format SCAN Scanning Informati ee gt Chan Filter Laser Sensitivity PMT Splitter Total Scan Time 14 Min 48 Sec 1 580BP30 Green 532m Normal 600V Lines Per Channel 1500 2 670BP30 Red 533nm Normal 600Y Image Size per Channel 2 861 MB Fig 3 1 Typhoon Trio Scanner Control window Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 19 3 Fluorescence scanning 3 3 About the Fluorescence Setup window Emission Filter list Fluorescen
12. ce Setup flor Typhoon Trio Laser list r Link With Use Image Emission Filter PMT Laser Sensitivity pz 1 5208P 40 CY2 ECL Blue FAM E 600 E Blue 488 7 Normal r 2 sera Cy3 TAMRA AlexaFluorb46 Normal T s sroer 3005 Noma El 4 610 BP 30 Deep Purple SYPRO Ruby E Y 600 E Green 532 v Normal y Channel Data Warning DALAK Zi If non optimal filter laser or beamsplitter combinations are Auto Link selected a warning is displayed before scanning is initiated Disable Warning Auto Link Mode Sensitivity Cea E Fig 3 2 The Fluorescence Setup window for the Typhoon Trio instrument 3 3 2 Selecting the emission filter You select an emission filter from the Emission Filter list for each scan you want to perform The Emission Filter list displays the filters table 3 1 that are installed in the Typhoon Trio instrument If you do not want to use an emission filter select None from the list IMPORTANT The laser and emission filter combinations listed in the Fluorescence Setup window are based on the excitation and emission maxima of the selected fluorochrome For most gel microplate and array samples these combinations provide the best scanning results However for membrane samples selecting a laser and emission filter combination that is Close to the excitation and emission maxima might not provide the best results because of the
13. e glass platen and sample lid and analysing or preprocessing the scanned image 3 1 Excitation modes The available excitation modes for fluorescence scanning are e Red excited mode Creates an image from a sample labelled or stained with a fluorescent dye or from a chemifluorescence sample that is excited at 635 nm e Green excited mode Creates an image from a sample labelled or stained with a fluorescent dye or a from chemifluorescence sample that is excited at 532 nm e Blue excited modes Blue 488 Creates an image from a sample labelled or stained with a fluorescent dye or from a chemifluorescence sample that is excited at 488 nm using full laser power This mode is recommended for samples that have low background levels ECL Excitation Creates an image from a sample labelled or stained with a fluorescent dye or from a chemifluorescence sample that is Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 17 3 Fluorescence scanning 3 2 Emission filters excited at 488 nm using reduced laser power This mode provides the optimum excitation for ECL Plus chemifluorescence blot samples and for samples with high background levels that can be excited at a lower laser power e Multilabel excited mode Creates an image from a sample labelled with up to four fluorescent dyes The dyes can be excited by one or more of the four laser choices 3 2 Emission filters The Typhoon instrument contains a stand
14. equate measures WARNING All repairs should be done by personnel authorized by GE Healthcare Do not open any covers or replace any parts unless specifically stated in the instructions WARNING If liquid is spilled on the equipment the power supply shall be disconnected immediately CE Certification This product complies with the European directives listed below by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request 73 23 EEC Low Voltage Directive e 89 336 EEC EMC Directive The CE logo and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instru ments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this man ual Note The Declaration of conformity is valid only for systems that are marked with the CE logo ce Contents Table of contents Preface 1 Introduction A heilen 12 1 2 System components and optional accessories 12 1 2 1 SYSTEM CIN PON Sida 1 2 2 Optional accessories 1 3 Scan acquisition modes nesssssssssssssseseeneennnnnennnnn 1 4 Before using the system e eeeeeeeeetnnnntttttttttnttcccet 2 Safety 2 1 Safety OVerview itane pend apt cedet aet 2 2 Laser light safety
15. her CE labelled GE Healthcare modules or other products as recommended Safety standards This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C22 2 No 61010 1 e UL61010 1 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Preface EMC standards This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 Class A emission This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 9 Preface 10 Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 Austria Tel 01 57606 1613 Fax 01 57606 1614 Belgium Tel 0800 73 890 Fax 02 416 8206 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central amp East Europe Tel 43 1 972 720 Fax 43 1 972 722 750 Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 Eire Tel 1 800 709992 Fax 44 1494 542010 Finland amp Baltics Tel 358 9 512 3940 Fax 4358 9 512 39439 France Tel 01 69 35 67 00 Fax 01 69 41 98 77 Germa
16. his message appears turn off the power switch on the right side of the Typhoon instrument wait a few seconds and then turn the instrument back on If the error happens repeatedly contact Technical Support See Assistance in the preface for contact information 41 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 23 4 Troubleshooting 4 1 Recycling 24 Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Technical Specifications 5 5 Technical Specifications 5 1 Regulatory requirements Safety standards This product meets the requirements of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C22 2 No 61010 EMC Standards This device meets the requirements of the EMC directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 class A emission This device complies with part 15 of the FCC rules lemission Operation is subject to the following conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may
17. in stain ROX EtBr SYPRO Red and SYPRO Ruby Transmits light between 655 nm and 685 nm and has a transmission peak centered a 670 nm Use this filter with fluorochromes such as Cy5 Transmits light below 526 nm Using this filter with some applications such as ECF blotting can reduce the background level This filter is used for storage phosphor scanning and is not normally used for fluorescence scanning 18 Optional emission filters are available from GE Healthcare and must be installed by qualified service personnel Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Typhoon model Fluorescence scanning 3 3 3 About the Fluorescence Setup window See the Typhoon User s Guide for details on how to start the Scanner Control software and set the scan parameters in the Scanner Control window You use the Fluorescence Setup window in Scanner Control to select the fluorescence parameters to use with the sample You can select from one through four sets of parameters to perform one through four scans This section describes the Emission Filter list and the Laser list in the Fluorescence Setup window for the Typhoon Trio and Typhoon Trio instruments For a description of how to set the PMT and sensitivity and how to link scans see the Typhoon User s Guide 3 3 1 Displaying the Fluorescence Setup window Inthe Acquisition Mode area ofthe Typhoon Scanner Control window figure 3 1 select Fluorescen
18. ment is available in several models This addendum provides an overview of the Typhoon Trio and Typhoon Trio models The Typhoon Trio instrument contains three internal lasers blue green and red The Typhoon Trio is a Typhoon Trio instrument with the additional 10 um pixel size scanning resolution The topics in this chapter are e Site requirements section 1 1 e System components and optional accessories section 1 2 e Scan acquisition modes section 1 3 e Before using the system section 1 4 Fig 1 1 The Typhoon Trio instrument For a detailed description of how to use the Typhoon Trio system see the Typhoon User s Guide Typhoon Trio User Guide Addendum 00 4278 46 Edition AB 13 1 Introduction 1 1 Site requirements 14 11 Site requirements Table 1 1 provides a summary of the Typhoon Trio instrument site requirements For additional site guidelines see the Typhoon Installation Instructions for the Microsoft Windows XP Workstation Table 1 1 Typhoon Trio system instrument specifications Parameter Value Power requirements 100 120 V 6 A 50 60 Hz 220 240 V 4 A 50 60 Hz Weight Approximately 160 kg 350 Ib Dimensions Width 11811cm 46 5 in Height 48 26cm 19in Depth 77 47cm 30 5 in Temperature 15 30 C 59 86 F Humidity Noncondensing 10 80 relative humidity 1 2 System components and optional accessories 1 2 1 System components The Typhoon Trio system includes the Typhoo
19. n Trio or Typhoon Trio instrument Scanner Control software Typhoon Direct Instrument Access software Ethernet cables power cords and tools The Typhoon instrument connects to the computer using an Ethernet connection The Typhoon Trio instrument contains the following three internal lasers e Bluelaser A solid state laser with a specified minimum output of 20 mW at 488 nm in a diverging beam e Green laser A green visible laser with a specified minimum output of 20 mW at 532 nm in a diverging beam e Redlaser A red visible laser with a specified minimum of 10 mW at 633 nm in a diverging beam The Scanner Control software which runs on the Microsoft Windows operating system contains the graphical interface that allows you to control the instrument In addition Internet Explorer is required for the Typhoon Direct Instrument Access software You use the Typhoon Direct Instrument Access software to perform several maintenance procedures which are described in the Typhoon User s Guide Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Introduction 1 1 2 2 Optional accessories See the Typhoon User s Guide for a description of the optional accessories for the storage phosphor and fluorescence scanning modes In addition the Typhoon multislide tray is available for the Typhoon Trio instrument The multislide tray allows you to scan up to 30 microarray slides at a time See the Typhoon Multislide User s Guide for a
20. ny Tel 0800 9080 711 Fax 0800 9080 712 Greater China Tel 852 2100 6300 Fax 852 2100 6338 taly Tel 02 26001 320 Fax 02 26001 399 Assistance Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 iddle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 etherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 orway Tel 47 815 65 777 Fax 47 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 Russia CIS NIS Tel 7 495 956 5177 Fax 7 495 956 5176 Spain Tel 902 11 72 65 Fax 935 94 49 65 Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 10 Fax 0848 8028 11 UK Tel 0800 515 313 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1 877 295 8102 When calling for assistance be prepared to supply the serial number of your instrument For contact by phone or fax please use one of the numbers below Alternatively an updated list of contacts is available on the Web at www gehealthcare com Typhoon Trio User Guide Addendum 00 4278 46 Edition AB Introduction 1 1 Introduction The Typhoon Trio instrument is a variable mode imager that produces digital images of radioactive fluorescent or chemiluminescent samples The Typhoon system consists of an instrument figure 1 1 instrument control software and a variety of accessories The Typhoon instru
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