DYE PRIMER MATRIX STANDARDS
Contents
1. gt Applied DYE PRIMER MATRIX STANDARDS AS Biosystems Product P N 401114G For Research Use Only Insert P N 4340088A Not for use in diagnostic procedures Printed in U S A These standards are used to generate the multicomponent matrix on the Applied Biosystems GENE SCANNER Model 362 Fluorescent Fragment Analyzer and Model 373A DNA Sequencing System The Analysis software uses the multicomponent matrix to automatically analyze four different color fluorescent dye labeled samples in a single gel lane The ready to use kit contains one tube each of the FAM blue C JOE green A TAMRA yellow G and ROX red T matrix standards Each standard contains 28 dye labeled DNA fragments ranging in size from 55 to 14 097 base pairs native agarose gels or from 37 to 14 079 bases denaturing acrylamide gels The standards are diluted with blue loading buffer for convenience in gel loading The final concentrations are 0 5 nM for FAM and JOE 2 nM for TAMRA and 4 nM for ROX dye concentrations are per double stranded DNA fragment Before use mix each tube well and centrifuge to collect the liquid at the bottom The standards are stable for six months at 2 6 C avoid freeze thaw cycles GENE SCANNER agarose application Load 5 uL of standard per lane Each standard must be loaded in a separate lane Refer to the GENE SCANNER User s Manual for gel preparation and electrophoresis conditions The matrix calculation is performed by t2. actice the DNA sequencing or fragment analysis methods may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 U S A Copyright 2002 Applied Biosystems All rights reserved GeneScanner GeneScan TAMRA Joe FAM and ROX are trademarks of Applera Corporation or its subsidiaries for use in the US and certain other countries Product Insert 12 17 2002 GUIDELINE INSERT S Applied FOLD INSTRUCTIONS BSS biok ystems Notes Specification is intended for full and half sheet inserts two sheet max Material 50 Offset White Dimensions Full Sheet 8 2 x 11 folded size 1 833 x 2 125 125 Dimensions Half Sheet 5 12 x 8 12 folded size 1 833 x 2 125 125 Two sheet inserts to have pages 2 and 3 facing each other After final folding upper lefthand corner of page 1 is to face out with title and part number displayed Folded inserts to be packaged as follows e Bundled in groups of 10ea e Shrink wrap 10ea groups for 100 inserts total e Label shrink wrapped bundles Part amp Rev font large enough to be legible at 8 feet Qty Manufacturer and Barcode 128 if appropriate e Seal shrink wrapped bundles in an appropriately sized corrugated shipper not to exceed 25 or have outside dimensions greater than 12 x 13 1 2 x 4 e Appropriately label shipper Part amp Rev font large enough to be legi
3. ble at 8 feet Description P O Qty Mfg Date Manufacturer Country of Origin and Barcode 128 if appropriate on at least one end panel 12 x 13 side NO Ol oN Tiia of the Insert Car trav Up ted Lines ot Intarmiatiany ou KL FIN CALAIS Folding instructions ARE NOT and MUST NOT BE PRINTED as part of this insert THIS DOCUMENT CONTAINS PROPRIETARY INFORMATION OF APPLIED BIOSYSTEMS AND IS TOBE RETAINED IN CONFIDENCE NOT TO BE REPRODUCED COPIED OR INCORPORATED IN ANY MANNER APPLIED BIOSYSTEMS 2002 t Product Insert 12 17 2002
4. he GENE SCANNER Analysis program as described in the User s Manual Sequencing System acrylamide application For each standard combine 2 5 uL of standard with 2 5 uL of formamide Heat each standard formamide mix at 90 C for two minutes to denature before loading Use a separate gel lane for each standard formamide mix NOTE DNA samples must not be stored in formamide for more than a few hours Refer to the 373A Sequencing System User s Manual for gel preparation and electrophoresis conditions The matrix calculation is performed using either the Sequencer Analysis software or the 672 GENESCAN Analysis software refer to the appropriate user s manual NOTE For the phone number of Applied Biosystems technical support in your area consult the GENE SCANNER User s Manual Ancillary Products Disclaimer This product is optimized for use in the DNA sequencing or fragment analysis methods covered by patents owned or licensable by Applied Biosystems No license under these patents to use the DNA sequencing or fragment analysis methods is conveyed expressly or by implication to the purchaser by the purchase of this product A license to use the DNA sequencing or fragment analysis methods for certain research and development activities accompanies the purchase of certain Applied Biosystems reagents when used in conjunction with an authorized DNA sequencing machine or is available from Applied Biosystems Further information on purchasing licenses to pr
Download Pdf Manuals
Related Search