Manual - Pipette.com
Contents
1. 1 Before connecting the AC adaptor to the electrical outlet check that its voltage setting corresponds to the voltage of your electrical outlet 2 Connect the AC adaptor to the electrical outlet as well as to the electrical fitting at the base of the handle of the Midi Plus Fig 1 Note The Midi Plus should only be charged with the AC adaptor provided 3 The length of the recharging time is 14 hours for a completely empty battery Note The Midi Plus should be disconnected from the transformer if it will be out of use for a lengthy period of time Fig 1 2 2 Low battery indicator When the battery is close to empty the battery capacity indicator starts to blink Fig 2 The unit can still be used for some time when the blinking starts However as the capacity of the battery decreases the blinking frequency of the indicator will increase until it is permanently ignited and the unit will need to be recharged If it is inconvenient to recharge the Midi Plus immediately the unit can be operated directly through the AC adaptor For maximum life time it is recommended to allow the battery to fully discharge before recharging it Fig 2 Note If the battery has been fully discharged it may be necessary to charge it for a short period of time before it is possible to operate the Midi Plus directly through the AC adaptor Note The Midi Plus should not be operated continuously with the AC adaptor connected 2 3 Elect2. proper vacuum level and preventing leakage The Midi Plus can also be used with the Biohit Proline 5 ml Cat No 780200 and 780300 and 10 ml Cat No 780310 tips Fig 3 The changeable nose cones and silicon adapters which allow the use of 5 ml and 10 ml tips are available as accessories Pipet adapters silicon adapter nose cone 711015 Adapter set for 5 ml tip 1 711016 Adapter set for 10 ml tip 1 The nose cone and silicon adapter can be changed by Fig 3 pressing down the locking button while gently turning the nose cone anticlockwise and pulling it out see 7 2 Changing the nose cone silicon adapter or filter 4 4 Filters Removing the nose cone exposes a hydrophobic filter 0 45 micron located at the top of the nose piece The filter protects the Midi Plus from accidental overaspirations It is strongly recommended to use only the original filters supplied by the manufac turer with the Midi Plus Both sterile and non sterile filters are available see 7 2 Changing the nose cone silicon adapter or filter Note The Midi Plus cannot be operated without a filter Wetting of filter due to overfilling will slow or prevent fluid movement If overfilling occurs change the filter immediately 4 5 Suspension hook The Midi Plus can be suspended for the duration of the pipetting by hanging it from the suspension hook Fig 4 The suspension hook is located on top of the unit Fig 4 5 OPERATING THE MIDI
3. 8 T MAINTENANGE notare 8 T DecohltabmnallolTsssotecs n ille ae 9 7 2 Changing the nose cone silicone adapter and filter 9 7232 Ghangirig the Datteries c to er esee es 10 9 TROUBEESHOOTING aan 11 WARRANTY INFORMATION 11 1 YOUR NEW BIOHIT MIDI PLUS The Biohit Midi Plus has been carefully designed and manufactured as a lightweight cordless pipetting controller It can be used with all plastic or glass pipets between 1 100 ml The linear speed control allows the aspiration and dispensing speeds to be adjusted easily and precisely to suit either large or small volume pipets The aspiration and dispensing speeds can also be fine tuned during operation by applying varying finger pressure to the operating buttons The Midi Plus has been designed so that it can be suspended for the duration of pipetting This option can be employed to reduce the strain associated with long operating periods Produc 710929 Biohit Midi Plus U S plug 2 UNPACKING AND PREPARING THE MIDI PLUS FOR USE The Midi Plus package contains the following items e Midi Plus pipetting controller e AC adaptor 0 45 um hydrophobic filters Instructions for use Quality control certificate Please check that all the items are included and nothing has been damaged during shipment 2 1 Charging the Midi Plus The Midi Plus is supplied fully charged However it is recommended to recharge the unit overnight before the initial use
4. Midi Plus Instruction Manual eh Sy LOIZIL US L qu 166ZIL sod es Aroneg SS diri L ie TTITIL PS MINS J iets 9uoo 9SON LIIZIL Poq ure cm 660c 1 suisnoy Apog LOOCIL AM Ol6clL ius d II6ZIL TWOI 60cIL Sunesodo e Diepume JOAOO Alone g CDEMES nd 1 K quiesse uoynq EI6ZIL 0d 1 amas Sunesodo TI6ZIL S9d6 opuosuou ung 1o L me oiqoudo1pAH LUITIL eoeid tum E oe Sup o ouoo osoN VIICIL DAJLA uornoouuo EIIZIL Atquiosse dung TIIZIL Jos SUNL ITIZILA quosse juounsnfpe poods dung Spare Parts CONTENTS 1 YOUR NEW BIOHIT MIDI PLUS eseeeeeeeer 2 2 UNPACKING AND PREPARING THE MIDPPEUS FOR LUS Bice lia 2 2 1 Charging the Midi Plus i 2 2 2 Low battery indicator un late 3 2 3 Electrical specifications i 3 SoMIDEPEUS MATERIAL S lata anali 4 4 MIDI PLUS DESCRIPTION i 5 4 1 Operating buttons x icai sae iaceo ioa pau E Feds Eee cedet cue ken 5 4 2 Speed adjustment wheel sees 5 4 3 Nose cone and silicon adapter esses 5 AA NOS tia elica 6 4 5 SUSPENSION OO 6 5 OPERATING THE MIDI PLUS Le 7 5 1 Adjusting the operating speed 7 5 2 Insetrting a pipet sia n a a ex h E ER OCse 7 SoA PaO e T 7 94 Belice IET 7 5 5 Other pipetting recommendations enerne 8 o STORAGE una
5. PLUS 5 1 Adjusting the operating speed Select the desired speed by turning the linear speed ad justment wheel Fig 5 The speed selection can be easily accessed as the height of the indicator column corresponds to the selected speed Note The aspiration and dispensing speeds can be fine tuned during operation by applying a varying amount of finger pressure to the operating buttons 5 2 Inserting a pipet Fig 5 Gently push a pipet into the silicone adapter and ensure that it fits securely To insert the pipet safely hold the Midi Plus from above the nose cone and the pipet from the top end of the tube 5 3 Aspiration 1 Select a speed and fill the pipet by pressing the aspirating button Fill the pipet just above the desired level 2 Remove the pipet from the sample container by drawing it up against the inside wall of the container 3 Adjust the volume of the sample by pressing the dispensing button until the meniscus is at the required level 5 4 Dispensing The Midi Plus offers two different dispensig modes 1 If the dispensing button is pressed only up to a point where a check can be felt the dispensing function will be based on the force of gravity and the liquid will simply flow out 2 Pressing the dispensing button further down engages the motor and activates the blow out function Note Finger pressure can be varied to fine tune the speed within each mode 5 5 Other pipetting recommendations
6. Use the instrument within the limits of its chemical resistance Never use with solvents the vapours of which are incompatible with the Midi Plus materials Never use with highly flammable liquids Observe the reagent manufacturer s safety information Never use the instrument in an atmosphere with a danger of explosion Never use force with the Midi Plus Should the instrument fail to function properly stop pipetting and check the Trouble Shooting section Use only the spare parts and accessories recommended by the manufacturer Operational and storage temperatures 10 C to 35 C 6 STORAGE The Midi Plus should not be stored for a long period of time without removing the pipet It is also recommended to disconnect the Midi Plus from the AC adaptor if the unit is not used for a lengthy period of time 7 MAINTENANCE The Midi Plus has been designed for easy in house maintenance However Biohit also provides complete servicing and repair including a service report and a performance certificate Please return your Midi Plus to the local representative for repair Before returning it make sure that it is free from all contamination Note Please advise the service representative of any hazardous materials which may have come to contact with your Midi Plus 7 1 Decontamination To decontaminate lightly spray the outside of the Midi Plus with the Biohit Proline Biocontrol decontamination spray cat no 724004 5 I an
7. d wipe dry with a clean cloth The nose cone and the silicon adapter can also be disassembled see 7 2 and placed into a beaker containing Biohit Proline Biocontrol and left there for at least 30 minutes for a complete decontamination These parts should then be rinsed with distilled water and dried 7 2 Changing the nose cone silicone adapter and filter To change the nose cone silicon adapter and filter first press the nose cone lock release button Fig 6 rotate the nose cone anticlockwise and pull out the nose cone and the silicon adapter The filter can be removed by simply pulling it out once the nose cone and the silicon adapter have been disen gaged Position the new filter with the coloured side upwards To reassemble insert the adapter and nose cone and twist the nose cone gently clockwise Both the nose cone and silicon adapters are autoclavable 121 C 1 atm Fig 6 Note It is recommended to change the silicon adapter every six months or when necessary to prevent a loss of vacuum Spare adapters can be ordered from your local Biohit distributor Also adapters which allow the use of 5 ml and 10 ml tips can be ordered separately Silicon adapter spare parts 712893 Standard silicon adapter for Biohit Midi Plus 712910 5 ml silicon adapter for Biohit Midi Plus 712911 10 ml silicon adapter for Biohit Midi Plus Nose cone spare parts PVDF Product 712109 Standard nose cone for Biohit Midi Plus 712107 5 ml no
8. el 7 812 327 5327 Sanghai Representative Office Fax 7 812 327 5323 Tel 86 21 6248 5589 E mail main biohit ru Fax 86 21 6248 7786 E mail info china biohit com U K Biohit Ltd France Tel 44 1803 315 900 Biohit S A Fax 44 1803 315 530 Tel 33 1 3088 4130 E mail info biohit co uk Fax 33 1 3088 4102 E mail commercial france biohit com Germany Biohit Deutschland GmbH Tel 49 6003 828 20 Fax 49 6003 828 222 E mail info biohit de BIOHIT PLC Headquarters Laippatie 1 FI 00880 Helsinki Finland Tel 358 9 773 861 Fax 358 9 773 86 200 E mail info biohit com www biohit com Due to Biohit s continuing R amp D effort specifications may change without prior notice Biohit innovations are covered by multiple patents and patents pending Midi Plus Instruction Manual 400503 01
9. harge Pipet drips Pipet not securely Reattach attached Silicon adapter or Reassemble change nose cone loose silicon adapter Silicon adapter or Replace pipet damaged Reduced operating Batteries worn Replace batteries time 9 WARRANTY INFORMATION The Biohit Midi Plus is warranted for one year against defects in materials and workmanship except the batteries 3 months Should your Midi Plus fail to function according to specifications at any time please contact the local Biohit representa tive immediately ANY WARRANTY WILL HOWEVER BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT MISUSE UNAUTHORIZED MAINTENANCE OF SERVICE OR NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE ACCIDENTAL DAMAGE INCORRECT STORAGE OR USE OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS OUTSIDE THEIR SPECIFICATIONS OR CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL Each Biohit Midi Plus is tested before shipping by the manufacturer The Quality Assurance Procedure guarantees that the Midi Plus you have purchased is ready for use Each Biohit Midi Plus is CE marked fulfilling the requirements of the EMC standards EN 55014 1993 and EN 55104 1995 12 U S A Japan Biohit Inc Biohit Japan Co Ltd Tel 1 732 922 4900 Tel 81 3 5822 0021 Toll Free 800 922 0784 Fax 81 3 5822 0022 Fax 1 732 922 0557 E mail sales biohit co jp E mail pipet biohit com Russia China Biohit OOO Finland Biohit Co Ltd T
10. rical specifications Batteries AA 3x 1 2 V 270 mAh NiCd Approx 7 hours service life when fully charged Charging time 14 hours for completely empty batteries AC adaptor Input voltage and main plug according to local requirements Output voltage 9 VDC 3 MIDI PLUS MATERIALS Suspenison hook ASA PC Handle cover release buttons Case ASA PC Handle cover ASA PC V4 ASA PC Ennii Speed adjustment wheel Nose cone ASA PC PVDF Silicone adapter Locking button POM Operating buttons ASA 4 PC Low battery indicator 4 MIDI PLUS DESCRIPTION 4 1 Operating buttons The Midi Plus has two operating buttons located at the front of the handle The upper button enables aspiration for as long as the button is held down The lower button allows for dispensing The aspiration and dispensing speeds can be fine tuned during operation by applying varying finger pressure to the operating buttons 4 2 Speed adjustment wheel The speed adjustment wheel is located at the upper part of the handle The desired speed is selected by turning the speed adjustment wheel 4 3 Nose cone and silicon adapter The pipet adapter provided with the Midi Plus allows the use of all standard plastic and glass pipets graduated and non graduated including Pasteur pipets The pipet adapter of the Midi Plus consists of the nose cone and silicon adapter The silicon adapter enables a pipet to fit securely in place thus creating a
11. se cone for Biohit Midi Plus 712108 10 ml nose cone for Biohit Midi Plus Note The Midi Plus cannot be operated without a filter Wetting of filter due to overfilling will slow or prevent fluid movement If overfilling occurs change the filter immediately Both sterile and non sterile filters are available 712912 Filter for Biohit Midi Plus non sterile 712913 Filter for Biohit Midi Plus sterile 1 pc Note If there is any unwanted leakage after the Midi Plus has been disassembled carefully check that all the components have been securely reattached Note Only use the original filters supplied by the manufacturer 7 3 Changing the batteries If the battery does not hold a sufficient charge for the proper operation of the Midi Plus follow these steps to replace the battery 1 Press the two release buttons towards each other Pull out the back cover to expose the battery compartment Fig 8 2 Insert the batteries according to the markings inside the battery compartment The positive end of the battery must be placed against the mark inside the compartment etc Fig 9 3 Replace the back cover 4 Dispose of the batteries appropriately Note Always replace the complete set of batteries Only use the original batteries supplied by the manufacturer 10 8 TROUBLE SHOOTING Reduced aspiration Filter blocked dirty or Replace the filter capacity or the Midi damaged Plus blocked Batteries low Rec
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