Model 66 & 77 Peristaltic Pump Manual
Contents
1. Prompt character will not change MOD BAT PMP DIS Request to set or query pumping mode set MOD BAT puts pump in batch mode MOD DIS puts pump in dispensing mode MOD PMP puts pump in pumping mode Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR o Prompt character will be Query MOD If pumping mode is batch response will be If space space BATCH CR If pumping mode is dispense response will be If space space DISPENSE CR If pumping mode is pump response will be If space space PUMP CR DIR CW CCW REV Request to set or query pumping direction set Harvard Apparatus Peristaltic Pump Model 66 77 DIR CW sets pumping direction to clockwise DIR CCW sets pumping direction to counter clockwise DIR REV reverses direction of current pumping direction Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR a Prompt character will be Publication 5387 001 Revision B Pump Operation Contd i Query MOD If pump direction is clockwise response will be If space space CW CR If pump direction is counter clockwise response will be If space space CCW CR Prompt character will not change VER Request for version of pumps embedded software Response for the present version will be If s2. ATD ONIOFF set query anti drip ENP ONIOFF set query end point dec MOD BATIPMPIDIS set query pumping mode DIR CWICCWIREV set query pumping direction VER query software version IN d sread TTL pin d OUT d lt ONIOFF gt output TTL pin d Publication 5387 001 Revision B Harvard Apparatus Peristaltic Pump Model 66 77 Table 1 RS 232 Commands Contd All spaces ignored Maximum 5 digits per integer float or time output gt lt response gt lt prompt gt response gt lt response gt lt response gt lt If gt lt message gt lt cr gt prompt gt lt f gt stopped lt lf gt gt pumping lt d gt between batches dis paused or stalled message gt bb unrecognized command bb NA com not presently applicable bb OOR entered value out of range bb lt text gt lt integer gt lt float gt lt time gt float gt ffffff f gt dl integer gt ddddd time gt d dd dd d gt 0111213141516171819 b gt space cr gt acio carriage return If gt ascii 10 line feed text gt any string of ascii characters legend we optional capital terminals lower case non terminals either or Publication 5387 001 Revision B
3. Endpoint Slowdown The pump slows as the setpoint is neared for improved dispense accuracy Anti drip At the completion of a dispense the pump is reversed to prevent any additional unwanted drops Publication 5387 001 Revision B General Description Contd 4 RS 232C Interface Remote computer control daisy chain dispense by weight interface with other laboratory equipment TTL RUN STOP controls through hand or footswitch or contact closures 5V signal to activate a relay TTL signal level input Non volatile memory The last settings are stored in memory Momentary loss of power The pump stops and displays flashes to alert the user The pump is designed so that the illuminated display will always indicate the sta tus of the pump and the appropriate units associated with the pump operation For example when dispensing the display shows the increasing volume with units of ml and run illuminated When the dispense is completed and the pump stops the run is switched off Harvard Apparatus Peristaltic Pump Model 66 77 Publication 5387 001 Revision B Harvard Apparatus Peristaltic Pump Model 66 77 Initial Set Up pa par N Read the manual Load tubing in pumphead Select the correct tubing and locate the tubing in the appropriate size tubing holder machined in the pump block Feed the tub ing around the pumphead by threading the tubing through the round scallop
4. Parity none Stop bis 2 Weighing Scale The pump is programmed to pump by weight when connected to a weighing scale A weight feedback loop is established automatically and the dispense which is in units of weight is controlled by the weight measured by the scale The weight feedback loop provides control independent of the pump action and guarantees very accurate dispenses a Press SET and 1 Press 1 again until SCALE is displayed press ENTER The display should read EtLEr Mettler OHAUS OHAUS SArTr Sartorius Repeat presses of 1 changes the display until the appropriate name is displayed Then press ENTER The display clears back to zero The weighing scale must also be configured METTLER PM Series Baud rate 2400 Parity even Pause 0 OHAUS GT Series Baud rate 2400 Auto print feature OFF Stable data only OFF Serial data frame Fr 7 SARTORIUS Basic Series E1200S E2000D MP8 4 series Code Settings 211 External print command without stability 225 2400 baud 234 Even parity 432 Beeper off 311 Weight only models with PLUS performance package Publication 5387 001 Revision B gt Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd 8 3 Daisy Chain Multiple pumps can be connected through a daisy chain and controlled by a single PC The PC is connected to the IN connector of the first pump
5. and the OUT of the first pump is connected to the IN of the second pump Daisy chain is repeated to connect all pumps After the connections are made each pump must be given an identifying address and the communication baud rate must be selected a Press SET and 1 Repeat pressing 1 until CHAIN is displayed press ENTER b Address The display now reads Adr 00 This display represents Address number The colons flash to indicate that a setting is required The numerical keypad is used to enter the address number from 0 to 99 Each pump must be given a different address number When the correct address number is displayed press ENTER G Baud Rate After pressing ENTER display now reads b 2400 that is baud rate 2400 bits second Repeat presses of 1 toggles through the baud rate selection b 300 b 600 b 1200 b 2400 When the required baud rate is displayed press ENTER The display clears to zero ready for dispensing 9 ENDPOINT SLOWDOWN This feature which slows the pump as the setpoint is approached and can be selected for improved accuracy a Press SET and 2 The display reads E OFF or E ON Each time 2 is pressed the states switch from ON to OFE Press ENTEOR when the required state is displayed 10 ANTI DRIP This feature reverses the pumphead at the completion of a dispense to prevent any unwanted extra drops falling into the dispense container a
6. to zero 7 RS 232 INTERFACE The RS 232 Interface Connectors are telephone jacks mounted on the rear panel and marked IN and OUT Through the RS 232 Interface the pump can be controlled remotely by a PC or can communicate with other laboratory devices such as a weighing scale Multiple pumps can be controlled by a single PC through a daisy chain inter connection between pumps The IN connector is used to connect the pump directly to a PC or other devices The OUT connector is used in the daisy chain and is connected to the IN connector of the next pump in the chain Harvard Apparatus Peristaltic Pump Model 66 77 8 PRINTER WEIGHING SCALE OR DAISY CHAIN SELECTION After the printer scale or daisy chained pump connection is made the pump must be configured to communicate to the connected device Configuration selection is made by pressing SET and 1 Repeat presses of 1 changes the display through Print Scale and Chain When the appropriate display is recognized selection is made by pressing the ENTER key Publication 5387 001 Revision B 4 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd 8 1 8 2 Printer a Press SET and 1 Repeat pressing key 1 until Print is displayed Press ENTER b Printer is connected to the IN connector then the pump communications interface is set Gi Printer Configuration Baud rate 2400 Word size 8
7. Enter the dispensed volume using the numerical keypad The display will show the entered numbers When the correct value is displayed press ENTER The pump will now automatically recalculate the calibration factor which is displayed with ml rev illuminated while the CAL key is depressed The dis play defaults to 0 0000 when the CAL key is released To review the new calibration factor press CAL The new value will be dis played for as long as the CAL key is depressed When your finger is removed the display will default to volume again Calibration dispenses should be over 10 ml so that drop size becomes insignificant Publication 5387 001 Revision B 5 Harvard Apparatus Peristaltic Pump Model 66 77 Auto Calibration Auto Calibration When the pump is connected to a weighing scale and the CAL mode is selected the pump will dispense liquid into the container on the scale and the calibration sequence is fully automatic When the RUN key is pressed the scale is tared and the pump displays a set of hor izontal dashes After taring the dispense proceeds The endpoint slow down and anti drip features are automatically selected When the dispense is completed the dispensed weight registered by the scale is read by the pump and used to automatically recalculate the calibration factor The calibration factor is now in units of weight revolution For scale correction see Section 8 2 Publication 5387 001 Re
8. Model 66 amp 77 Peristaltic Pump Series User s Manual Model 66 Small Peristaltic MA1 55 77606 Model 77 Large Peristaltic MA1 55 7777 HARVARD APPARATUS Publication 5387 001 Revision B 5 Harvard Apparatus Peristaltic Pump Model 66 77 Table of Contents SUBJECT PAGE NO Fable sof Cmte nits sss exiaescidatsiieasdcdesices st thas spas cigstesiensasatessasevnsteatcaaueese 1 General Information Warranty and Repairs cccseeeeesereeeeeeees 2 3 Technical Specifications sisisi resinae a i 4 General Descriptions siscscscessacssessestanesth seven seven sevenesveneoeesnveesencesssuesions 5 6 Ninttial Sets Up asses feces E steectelvateatc vey dees E E 7 8 Calibration a ra vivcesee red cies ois aei cred cee lives ane eee 9 10 Pump PETAtlOM cisccsedseieesetsscesesnasteseaegceceseseegarets steve cetestesevetonnelesscueese 11 PLOW Rea aa E RRE O E tees 11 Vol me Settings sas sist iv ninne e EE RER 11 Select Pumping Mode s scs sesccsacscscvasssessectvenccastsenatesnvesnesesione ses 11 Pamp Modereonen ani aa A aE 11 Dispense Mod iriesii sirine a E EAE 11 Batch Modestus Ea 11 12 RS 232 Intertace irn E ROR 12 Printer Weighing Scale or Daisy Chain Selection 12 14 Endpoint Slowdowth iii isscatsccecegussteecsesiecuaeveviat senepsetesesseveuienstes 14 Pant DE o svvdveneesea say tues EE T E E a T 14 TT E E E E A 15 RS 232 Interface Connections ccscecesseeseeseeeeeeeeeseeeeaeeeeaees 15 16 Interface
9. Press SET and 3 Display reads A ON or A OFF Pressing 3 changes from ON to OFF Press ENTER for displayed function Publication 5387 001 Revision B Pump Operation Contd 4 11 TTL The pump has a nine pin female connector for TTL input outputs Figure 1 11 1 Input a Momentary contact closure eg footswitch Momentary closure between pin 1 ground amp pin 6 causes the pump to change state that is from RUN to STOP or from STOP to RUN b Contact ground eg timer or relay Circuit from pin 7 to ground causes the pump to RUN STOP Rising edge Pump runs Falling edge Pump stops c Pins 6 7 8 and 9 are software readable NOTE Footswitch and timer are inactive during remote operation 11 2 Output a Pin 1 is ground Pins 2 3 4 are software controlled ON 5V OFF 0 V Pin5 5V 12 RS 232 INTERFACE The peristaltic pumps Models 77 and 66 use an RS 232C interface to communicate with a computer The same communication system is used by most terminals modems and many laboratory instruments 12 1 Computer to Pump Connection Any device with RS 232C communication such as a PC has a serial or COM port usually 25 pin male connectors A special cable fitted with a 25 pin female connector is used to connect the serial port to the pump which is equipped with a telephone socket marked RS 232 IN The cable wiring diagram is shown below in Figure 2 Harvard Ap
10. Query and Command Reference sssersrssissesen 16 18 Ma intetant siening na E E a EE 18 RS 232C Pump Chain Communications sssssssssseeseeerreesee 18 24 Table T sarea stent hee ste E E a eee 25 26 Publication 5387 001 Revision B Harvard Apparatus Peristaltic Pump Model 66 77 General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above lim itation may not ap
11. at is No parity 8 data bits 2 stop bits INTERFACE QUERY AND COMMAND REFERENCE All spaces transmitted are ignored Transmissions to a pump terminate with CR Carriage Return Format is address query or command CR Address is Pump address 0 99 Default is 0 Query format address XXX CR XXX 3 letter e g 12 VOL CR Pump 12 what is volume setpoint Command format address VOL CR can be an integer or decimal point e g 12 VOL 19 84 CR Pump 12 set volume setpoint at 19 84 ml d Query Commands Publication 5387 001 Revision B 4 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd RUN SIP VLA CLA VER RAT KOK VOL OK OK CAL P k NUM integer max 99999 INT time ATD on off ENP on off MOD BAT PMP DIS DIR CW CCW REV IN OUT d ON OFF Return to keyboard Start pump Stop pump Query volume accumulator Clear volume accumulator Query software version Set Query rate Set Query target volume Set Query calibration factor Set Query batch limit Set Query batch wait interval Set Query anti drip Set Query endpoint slowdown Set Query pump mode Set Query pump direction Read TTL pin Output TTL pin d ON 5V OFF OV or integer or decimal point e X XX XX hr min sec Interface Responses The pump responds back to the PC after each query or command pump output prompt response message bb bb b
12. ated address responds with its prompt c Optional pump address command CR Send command The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0 The following are the allowable commands and their responses during execu tion After each command is executed the addressed pump sends its prompt If a command is not recognized by an addressed pump the pump will respond with the following lf space space CR KEY Leaves remote mode turning remote light off and re enabling keyboard footswitch and timer No response Prompt character not changed RUN Starts pumping according to the present setting of the pump If pump is already pumping command has no effect No response Prompt character changed to gt STP Stops pump if it was running If pump is already stopped command has no effect No response Prompt character changed as follows _ If pump was running prompt gt new prompt changed to paused If pump was executing a batch interval prompt new prompt changed to e stopped Publication 5387 001 Revision B J Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd VLA Request for volume accumulator Response is of the following format lf fF CR Prompt character not changed CLA Request to zero volume accumulator If t
13. b integer float time text integer float Publication 5387 001 Revision B response prompt If adr pump stopped If adr gt pump running CW If adr pump stopped interval between dispenses If adr pump stalled or paused If line feed adr address of pump 0 99 If message CR bb NA not available OOR out of range text any string of ASCII characters ddddd d 0 9 FEE f integer or decimal point 7 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd _ ba yw time d dd dd hr min sec b blank lf ASCII 10 line feed CR ASCII 13 carriage return MAINTENANCE No maintenance is required except for general cleaning with water and mild detergent RS 232C PUMP CHAIN COMMUNICATION Configuration of Pump Chain Each pump in the pump chain needs to be individually configured as a mem ber of the pump chain To configure a pump two items of information are needed baud rate of the host and address of the pump Allowable baud rates are 300 600 1200 or 2400 One baud rate is selected for the entire chain to use Each pump in the chain is assigned a unique address from 0 to 99 Enter the configuration into the pump as follows e Press keys SET 1 and continue to press 1 until display is shown as SCALE e Press ENTER Display reads ADR nn e Enter 1 or 2 digit address then ENTER Display reads b baud e Press 1 until desired baud rate
14. e set 6 1 Number of Dispenses a Press SET and NUMBER Display shows the last setting Use numerical keypad to enter required number of dispenses b When correct number is displayed press ENTER Display clears ready for dispense c Continuous Dispensing If NUMBER is set to 0 the pump interprets this as an infinite number of dispenses and continues to dispense until manually stopped Publication 5387 001 Revision B Pump Operation Contd 6 2 Time Interval Between Dispenses a Press Set INTERVAL Display reads 0 00 00 representing time interval in Hours Minutes Seconds The colons will flash to indicate ready for setting b The interval is entered from the numerical keypad and displayed as entered These numbers are displayed from the right For example For interval 1 hour 15 minutes 23 seconds enter 11523 For interval 49 seconds enter 49 Minimum time interval is 1 second c When the correct value is displayed press ENTER d Press RUN to start Display reads volume and automatically stops at the setpoint The display changes to show the number of completed dispenses After one second the display changes again to time interval counting up in seconds When the interval setpoint is reached the pump automatically moves on to the next dispense e If the RUN STOP key is pressed during the time interval the RUN STOP does not act as a pause but stops the batch dispense mode and resets the batch number
15. he pump was paused it will return to its stopped state If pump is running a Not Applicable response will be given If space space NA CR a No response Prompt character will be RAT lt float gt Request to set or query rate setting L Set rate RAT rate Rate is of format fFF If rate is accepted and valid rate will become the new rate Rate will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the rate is invalid an Out of Range response will be given lf space space OOR CR a Prompt character will be Query rate RAT Response is of the following format If fF CR Prompt character will not change VOL lt float gt Request to set or query volume setting k Set volume VOL volume Volume is of format fF If volume is accepted and valid volume will become the new volume Volume will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the volume is invalid an Out of Range response will be given If space space OOR CR a Prompt character will be Publication 5387 001 Revision B Pump Operation Contd 2 Harvard Apparatus Peristaltic Pump Model 66 77 Query volume VOL Response is of the following format lf fF CR Prompt character will not change CAL lt float gt Request t
16. in the pumphead Manually rotate the pumphead and simultaneously feed the tubing around the moving pumphead through the scallop Fasten the free tubing into the second tubing holder The tubing should be snug around the pumphead but not too tight Rotate the pumphead manually a few revolutions to ensure free movement Model 77 Model 65 3 4 Tubing Size Tubing Size A 1 4 1D B 1 8 ID A 1 8 ID B 146 1D Tubing recommendations Norprene Tygon R 1000 C flex Viton Silicone I D 1 16 1 8 1 4 inch wall thickness 1 16 inch Registered trademark Norton Co Norton Co Concept Inc E I duPont de Nemours and Co Turn on the main power switch located on the rear panel above the fuse hold er The display should initially illuminate all LED s followed by 0 0000 flash ing to indicate the pump is ready Press any key to stop the flashing and make ready for entries Publication 5387 001 Revision B 3 Harvard Apparatus Peristaltic Pump Model 66 77 Initial Set Up Contd an we Note that U S models are set for 115 VC Overseas models should have an appropriate plug installed and the voltage selector switch inside the pump will be correctly adjusted Polarity for international line cord Brown high Blue neutral Green ground O The pump is delivered with a programmed calibration factor corresponding to 1 8 inch I D tubing For accurate dispenses the
17. is shown then ENTER Display now reads 0 0000 Remote Control When a pump is sent a command or a request is made for its prompt it enters remote mode indicated by the remote light on the pump s display During remote mode the KEYBOARD FOOTSWITCH AND TIMER ARE DIS PLAYED The keyboard footswitch and timer can only be re enabled by either sending a KEY command see below or turning the power to the pump OFF and then ON again A pump will not accept pump chain communication while it is in a setting mode entered when user presses the SET key or during calibration Any settings changed from remote mode will not be stored in the pump s non volatile memory When remote mode is exited via the KEY command any changes to the settings of the pump will cause all settings including those changed during remote mode to be stored in non volatile memory Publication 5387 001 Revision B Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Contd Prompts A prompt is a string of ASCII characters sent by a pump l f line feed 1 or 2 digit address prompt character prompt characters spump stopped gt pump running pump paused or stalled pump between batches Commands Each command sent to the pump chain is a string of ASCII characters with the following formats and meanings all spaces ignored by pump a CR Stop all pumps b pump address CR Request for prompt The pump with the indic
18. mphead rotation and to the num ber of steps moved by the stepper motor The microprocessor maintains count of the number of steps and the motor can be microstepped with up to 6400 microsteps per revolution of the pumphead This fine control permits great dis pensing precision both of flowrate and volume dispensed In addition to compen sate for variations in tubing and flow of different liquids a simple to use calibration method is built in which will automatically recalculate the volume dispensed per revolution thereby ensuring greater accuracy Microprocessor control allows many options to be included in the pump such as volume dispense and repetitive batch dispenses Via RS 232C the pump can be interfaced with many laboratory devices and up to 100 pumps can be controlled by one PC through a daisy chain system included in the pump The digital communication interface can be used to connect the pump directly to a weighing scale to establish a feedback loop and dispense by weight A TTL input output is also included Features Pump Mode Pump runs continuously at set flow rate Dispense Mode Pump runs at set flow rate to the set volume Batch Mode Multiple dispenses at set flow rate and set volume The number of dispenses and the time delay interval between dispenses can be selected Calibration Mode Used to improve dispense accuracy Precise measure ment of volume or weight dispensed per pumphead revolution
19. o set or query calibration setting 1 Set calibration CAL calibration Volume is of format fF If calibration is accepted and valid calibration will become the new calibration Calibration will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the calibration is invalid and Out of Range response will be given If space space OOR CR a Prompt character will be Query calibration CAL Response is of the following format lf fF CR Prompt character will not change NUM lt integer gt Request to set or query batch limit number I Set number NUM number Number is of format ddddd If number is accepted and valid number will become the new number Number will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR a Prompt character will be Query rate NUM Response is of the following format lf ddddd CR Leading zeros will be replaced by spaces Prompt character will not change Publication 5387 001 Revision B Pump Operation Contd 3 INT lt time gt Request to set or query batch interval time 1 Set Interval INT interval Interval is of format d dd dd hr min sec If interval is accepted and valid interval will become the new interval Interval will not be accepted if the pump is running A Not Applicable response will be given If s
20. pace space 77V1 0 CR IN d Request to read in the TTL logic value at the specified pin d on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be If space space ON CR If the pin level is low response will be If space space OFF CR If the pin specified is invalid an Out of Range response will be given If space space OOR CR Prompt character will not change OUT d lt ON OFF gt Request to set the TTL logic value at the specified pin on the external 9 pin D SUB connector Valid pin numbers for output are 2 3 and 4 Harvard Apparatus Peristaltic Pump Model 66 77 If the pin specified is valid no response is given If the pin specified is invalid an Out of Range response will be given If space space OOR CR Prompt character will not change Publication 5387 001 Revision B 2 Harvard Apparatus Peristaltic Pump Model 66 77 Table 1 RS 232 Commands command gt lt adr gt lt cmd gt lt CR gt adr gt ad cmd gt KEY sreturn to keyboard RUN start pump STP stop pump VLA query volume accumulator CLA clear volume accumulator RAT lt float gt set query rate VOL lt float gt set query target volume CAL lt float gt set query calibration NUM lt integer gt set query batch limit INT lt time gt set query batch wait interval
21. pace space NA CR If the interval is invalid an Out of Range response will be given If space space OOR CR a Prompt character will be 2 Query Interval INT Response is of the following format If didd dd CR Prompt character will not change ATD ON OFF Request to set or query anti drip setting 1 Set ATD ON turns anti drip function ON ATD OFF turns anti drip function OFE Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR Prompt character will be 2 Query ATD If anti drip function is ON response will be If space space ON CR Harvard Apparatus Peristaltic Pump Model 66 77 If anti drip function is OFE response will be If space space OFF CR Prompt character will not change ENP ON OFF Request to set or query end point deceleration setting 1 Set ENP ON turns end point deceleration function ON ENP OFF turns end point deceleration function OFF Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR a Prompt character will be Publication 5387 001 Revision B Pump Operation Contd 2 Query ENP If end point deceleration function is on response will be If space space ON CR If end point deceleration function is off response will be If space space OFF CR
22. paratus Peristaltic Pump Model 66 77 To connect the pump a Connect the cable to the computer and pump b If appropriate connect all pumps together in the daisy chain from OUT of pump 1 to IN of pump 2 etc G Tighten retaining screws Publication 5387 001 Revision B Pump Operation Contd 3 Harvard Apparatus Peristaltic Pump Model 66 77 13 12 2 12 3 12 4 Setting the Baud Rate The RS 232C interface is bidirectional with data transmitted one bit at a time The rate of transmission is the baud rate number of bits per second The pumps operate at 300 600 1200 2400 a Press SET and 1 Continue to press 1 until CHAIN is displayed Press ENTER The display will now read the pump address ADr 01 b Enter the correct address location and ENTER The display will now show the current baud rate setting c Repeat presses of 1 changes the rate b 300 b 600 b 1200 b 2400 When the required rate is displayed press ENTER This procedure must be followed for every pump connected to the computer Front Panel Indicator After the pump is configured and the RS 232C connection is made the pump is ready for remote control After receiving the first command to its address the pump keypad footswitch and timer are disabled and the REMOTE light is illuminated Local control is re established using the KEY command Communication RS 232C form
23. paratus Peristaltic Pump Model 66 77 Volume Size Weight Power Leakage to Ground Ground Resistance Drive Motor Flow Rate Minimum Maximum Step Rate Minimum Maximum Maximum Back Pressure Accuracy Reproducibility Interface Selectable Baud Rates Adjustable from 4 to 100 cc stroke 229 x 206 x 89 mm 9 x 8 1 8 x 3 1 2 in 3 6 kg 7 9 Ib 115 230 volts 50 60 Hz via internal selector switch 30 watts Fuse 0 5A S B typically less than 20 micro amps typically less than 0 5 Q Microprocessor controlled stepping motor 0 01 ml min 200 ml min 27 3 seconds per step 416 7 microseconds per step 30 psi 1 1 Bidirectional RS 232C 300 600 1200 2400 Publication 5387 001 Revision B Adjustable from 4 to 100 cc stroke 241 x 206 x 127 mm 9 1 8 x 8 1 8 x 5 in 5 1 kg 11 1 4 Ib 115 230 volts 50 60 Hz via internal selector switch 30 watts Fuse 0 5A S B typically less than 20 micro amps typically less than 0 5 Q Microprocessor controlled stepping motor 0 01 ml min 1 8 in 1 D 700 ml min 1 4 in I D 27 3 seconds per step 416 7 microseconds per step 30 psi 1 1 Bidirectional RS 232C 300 600 1200 2400 s Harvard Apparatus Peristaltic Pump Model 66 77 General Description The Harvard Model 77 and Model 66 are microprocessor controlled stepper motor driven peristaltic pumps The volume dispensed is proportional to the pu
24. ply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service depart ment The customer is responsible for shipping charges Please allow a rea sonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remain der of the original warranty period dating from the purchase of the origi nal device Publication 5387 001 Revision B s Harvard Apparatus Peristaltic Pump Model 66 77 General Information Contd This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Publication 5387 001 Revision B Models 66 77 Specifications Model 66 Model 77 Harvard Ap
25. pump should be calibrat ed to match the users conditions Publication 5387 001 Revision B s Harvard Apparatus Peristaltic Pump Model 66 77 Calibration Calibration 1 2 3 4 5 6 7 8 9 10 Load the pump with the required tubing and liquid to be pumped Prime the pump by pressing RUN The pump is initially configured to run until stopped with the STOP key Press SET and CAL to select the Calibration mode ml rev will be illumi nated and flashing Enter the approximate calibration factor from the table below using the numerical keypad When the correct number is displayed press ENTER Approximate calibration factors Tubing I D inch 1 4 5 85 N A 1 8 1 22 0 88 1 16 N A 0 22 Tubing wall thickness is 1 16 inch The pump should be run in the Calibration mode for a more accurate cali bration factor The pump can be operated at the preset rate and volume set tings or more appropriate RATE and VOLUME settings can be entered after reading Pump Operation Press SET and CAL to select Calibration mode Press RUN The pump will start the display will show the increasing volume pumped The volume displayed is calculated based on the programmed cali bration factor and may not be accurate When the dispense is completed the pump stops automatically The Run light is shut off and ml is flashing Measure accurately the dispensed volume
26. vision B 5 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation The pump must now be set for flow rate volume and the operational mode selected 1 2 3 4 FLOW RATE a Press SET and RATE The ml min LED will flash indicating that the value with these units is ready for updating b The flow rate value in units of ml minute is entered from the numerical keypad As the numbers are entered they will be displayed c When the correct value is displayed press ENTER VOLUME SETTING a Press SET and VOLUME ml unit indicator will flash b Enter value of volume to be dispensed G Press ENTER Display defaults to zero SELECT PUMPING MODE a Selection is made by pressing MODE The present mode selection is displayed as either Pump Dispense or Batch b Press the MODE key until the required mode is displayed PUMP MODE Continuous pumping Change flow rate if required b Press RUN STOP to commence Display shows increasing pumped volume ml units a To pause press the RUN STOP key Press RUN STOP key again to restart The pump continues and displays the accumulated volume DISPENSE MODE Pump runs at set rate to a set volume and automatically stops a Use SET RATE and SET VOLUME keys for setpoint entries BATCH MODE This mode is for repetitive dispenses of a set volume The number of dispenses and time delay interval between dispenses can b
Download Pdf Manuals
Related Search