Pressure Interface
Contents
1. 14 TW LN E SP RED EE O DR 14 o ens E i ii 16 ARA RR RED RAE E 17 CAI UE Sa 17 O ELITES e E 18 26 1 Force calibration dina it 19 3 6 2 Pressure Calibration PROCE QUITE sida Sd 22 3 6 3 pH Calibration Procedure optional rna aaia a 25 3 6 4 Analog Record Out Select o 26 e E E 27 SC TEMPERATURE DIFFERENCE ORE ira 27 PA EE EEE ani 28 305 3 FACTORY DIAGNOS TIC ia ATE east EEEE 28 Appendix 1 System Specifications nssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn mennan nn nnnm nnmnnn a na 29 4 PRESSURE INTERFACE USER MANUAL SAFETY The Pressure Interface has been designed for use only in teaching and research applications It is not intended for clinical or criti cal life care use and should never be used for these purposes Nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock e Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on2. Myograph see figure 2 3 to the P1 outlet on the cap of the buffer bottle see fig 2 3 e Connectthe perfusion outlet at P2 on the Pressure Myograph Unit see figure 2 3 to the P2 inlet on the Pressure Interface see fig 2 3 Figure 2 3 Pressure Interface tube connection CHAPTER2 11 NOTE TO FILL THE TUBING AND AVOID AIR BUBBLES MOUNT THE CALIBRATION SHUNT TUBE AS DESCRIBED IN CHAPTER 5 FILL THE P1 SCHOTT BOTTLE WITH A MAXIMUM OF 400 ML OF BUFFER IN THE PRESSURE MENU SET THE PRESSURE P1 AND P2 AS FOLLOW P1 150mm Hg P2 7Omm Hg Set the Pressure ON Set Flow ON Let the pressure regulator run until no air bubbles are visible in the perfusion circuit IMPORTANT NEVER RUN AIR BUBBLES THROUGH A VESSEL AS THIS MAY DAMAGE IT NOTE II NEVER FILL THE BUFFER BOTTLE WITH MORE THAN 400 ML OF BUFFER AS HIGHER VOLUMES MAKES PRECISE CONTROL OF THE PRESSURE MORE DIFFICULT 2 6 Extra perfusion inlet outlet The extra perfusion inlet outlet 3 way valves on the Pressure Myograph allow the introduction of special agents or reagents to the perfusion circuit during an experiment They are situated at P1 and P2 on the Myograph Chamber and the small handle on top of the valves controls the flow direction 2 7 Connecting superfusion flow optional Creating a superfusion flow in the Pressure Myograph Chamber requires an external peristaltic pump which is not part of the basic Pressure Myograph System Contact DMT f
3. NEXT STEP pH1 CALIBRATION Step no 1 2 Apply buffer with high pH value to the pH and Temp Probe When the pH reading is stable go to next step pH1 readings 1863 NEXT STEP pH1 CALIBRATION Step no 2 4 Apply buffer with the Low pH value to the pH and Temp Probe When the pH reading is stable go to next step pH1 readings 883 pH1 CALIBRATION Step no 4 The pH1 sensor is now calibrated if OK go to next step else repeat the calibration pH1 value 4 00 Probe Temp 25 0 NEXT STEP NEXT STEP CHAPTER3 25 3 6 4 Analog Record Out Select optional The RECORD OUT SELECT sub menu determines how data is sent to the BNC analog outputs at the back of the Pressure Interface There are 4 analog outputs Each output is individually programmable Any change made to RECORD OUT will only affect the data collected by the external data acquisition system such as an AD Instruments PowerLab So itis important to check the data acquisition setup when a change is made to the analog output with this setting The analog output works in range of 2 5V to 2 5V Use SELECT to select the OUT channel number to be changed Press ENTER to go to the output setup Use SELECT to select the line to be changed Use the arrow keys or DEFAULT to change select a new setup 26 PRESSURE INTERFACE USER MANUAL SELECT REC BNC ANALOG OUT Select Record Out1 Select Record Out
4. the apparatus e Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Only use secure industry standard connectors and tubing for pressure connections Faults defects and mistakes due to wrong connections void warranty We are not accountable for results and mistakes due to inappropriate pressure hookup e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time The Pressure Interface is delivered with an external 100 240VAC to 24VDC ADAPTER Protect the power ADAPTER and cord from being walked on or pinched Particularly at power plugs and the point where they con nect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped SAFETY 5 UNPACKING PRESSURE INTERFACE AND MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Pressure Myograph System for damage which may have occurred during handling and shipping If you suspect any kind of damage please con
5. the oxygen supply pipe on the Pressure Myograph Chamber cover to an adjustable oxygen supply See figure 2 2 Pipe for oxygen supply 12 PRESSURE INTERFACE USER MANUAL 2 10 Connecting a suction device Connect the large pipe on the Pressure Myograph Chamber cover to a vacuum pump via a suction bottle and a vacuum valve as illustrated in fig 2 1 The internal diameter of the silicone tube used for this connection must be 2 mm A 2 mm tube is not included with the Myograph System 2 11 3 Way valve adjustments The 3 way valves on each side of the Pressure Myograph Unit at P1 amp P2 have three different settings to control the inlet outlet flow to the Pressure Myograph Chamber The flow settings are illustrated in fig 2 4 and fig 2 5 Figure 2 4 Three way valve flow settings at P1 Figure 2 5 Three way valve flow settings at P2 CHAPTER 2 13 CHAPTER 3 THE PRESSURE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Pressure Interface 3 1 General description of how to navigate the touch screen Menus on the Pressure Interface are all accessible by a touch screen To access a menu simply touch the corresponding button on the screen When a setting needs to be changed press the relevant SELECT button or in the display below touching the ON or OFF buttons The line selected will turn blue indica
6. 2 Select Record Out3 Select Record Out4 SELECT SELECT SELECT SELECT SELECT REC BNC ANALOG OUT Select Record Out1 Select Record Out2 Select Record Out3 Select Record Out4 RECORD TOUTPUT SET Record 1 Force Rec 1 out 2 5V 100mN Rec 1 out 2 5V 100mN DEFAULT RECORD 1 OUTPUT SET Record 1 Force Rec 1 out 2 5V 100mN Rec 1 out 2 5V 100mN DEFAULT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT The following parameter in the Pressure Interface can be selected as output on the Analog channel Force Probe temperature Chamber temperature pH1 Pressure regulation ON OFF Flow ON OFF Optional Digital output 1 Digital output 2 Digital input 1 Digital input 2 Pressure 1 Pressure 2 When the parameter is selected press ENTER to save the value The output range is 2 5V to 2 5V The user can select the parameter value that gives 2 5V and the value that gives 2 5V In this example 100mN is 2 5V and 100mN is 2 5V Selecting asymmetrical values is also valid They can be 2 5V OmN and 2 5V 100mN NOTE REMEMBER TO PRESS THE ENTER KEY TO SAVE THE LINE CHANGES TO MEMORY 3 6 5 Interface settings The INTERFACE SETTINGS sub menu in SETTINGS has an additional 3 sub menus These 3 additional sub menus are TEMPERATURE DIFFERENCE pH SETUP FACTORY DIAGNOSTICS 3 6 5 1 TEMPERATURE DIFFERENCE OFFSET T
7. ACE SETTINGS 18 PRESSURE INTERFACE USER MANUAL 3 6 1 Force calibration Procedure Figure 3 1 Weight Calibration Set up gs As Yi Figure 3 2 Illustration of how to fit the balance just behind the glass cannula fixation plate CHAPTER3 19 Enter the FORCE CALIBRATION sub menu to begin the transducer calibration The display will show the force calibration procedure Step 1 The calibration procedure is listed in 6 individual steps and needs to be performed one at a time to calibrate the system Step 1 involves setting up the chamber for calibration Fill the cnamber to a normal level with double distilled water Press NEXT STEP Step 2 Involves setting up the calibration kit for the actual weight calibration Make sure that the tip of the calibration bridge arm is placed behind the glass cannula fixation plate as illustrated in fig 3 71 Carefully move the calibration bridge until the tip of the arm is placed freely behind the glass cannula fixation plate It should not be touching the fixation plate Press NEXT STEP when the calibration kit has been properly placed Step 3 This step initiates the heating process for the chambers In order for the calibration to be accurate the transducers must be heated to the same temperature to be used in your experiment This allows for heat induced expansion of the electronic parts in the transducer Otherwise inaccurate readings and transducer drift
8. ALLED WITH DRIVERS FOR THE DIGITAL CCD USB CAMERA FOLLOW THE PROCEDURES IN SECTION 2 2 TO SETUP THE PRESSURE MYOGRAPH SYSTEM If you have not purchased a computer from DMT follow the MyoVIEW Il Quick Installation Guide to install MyoVIEW Il on your own computer 2 2 Setting up the complete Pressure Myograph System This section describes how to connect the cables in the Pressure Myograph System as illustrated in figure 2 1 Before connecting any of the myograph equipment ensure that for the 110P Pressure Myograph system that the USB camera is connected to your PC For the 111P Pressure Myograph system please connect both USB cable of the DMT microscope to the computer Install the MyoVIEW Il Data Acquisition Software on your PC as described in the MyoVIEW II Quick Installation Guide NOTE BEFORE PROCEEDING WITH THE CONNECTION PROCEDURE MAKE SURE THAT THE PRESSURE INTERFACE THE MICROSCOPE AND THE PC ARE SWITCHED OFF 1 Pressure Myograph Unit to Pressure Interface connection Connect the Pressure Myograph Unit to the Pressure Interface by using the grey 25 44 pin connection cable The end of the cable with the temperature probe is connected to the Pressure Myograph Unit 2 Pressure Interface to PC connection Connect the Pressure Interface to the computer with the USB cable from the back panel of the Pressure Interface to the USB port on the computer 3 Pressure Interface and oxygen supply connection Connect the Oxygen inlet on the
9. DMI DANISH MYO TECHNOL Ost PRESSURE INTERFACE FOR PRESSURE MYOGRAPH SYSTEMS ae kre 1 Ro qu un i 2 3 1 LL o gt Eq User Manual Version 1 1 2 PRESSURE INTERFACE USER MANUAL PRESSURE INTERFACE FOR PRESSURE MYOGRAPH SYSTEMS TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 35 are trademarks of ADInstruments Pty Ltd Danish Myo Technology A S reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the Pressure Interface No part of this document may be reproduced by any means without the prior written permission of Danish Myo Technology A S Copyright O Danish Myo Technology A S DANISH MYO TECHNO Low TRADEMARKS 3 CONTENTS MN AS assassina i e se ce aa wc les E ee re A ce i ice a EA 3 A RE REP SE E 5 Unpacking the Pressure Interface and Myograph Systeim c sssccssssseccsensseeccnnssecennnseeconsseeeeonssseeeeasseenenssseeeenssseeeenssssenennsssens 6 Chapter 1 cca 7 od PRC SUE 7 Lo Pressure Mio sra o
10. ER MANUAL Icon for Pressure ON OFF Icon for Flow ON OFF Icon for Heater ON OFF OA CT Icon for Buzzer ON OFF MAIN MENU 00 00 00 g 1 Pressure P1 122 0 mm Hg Pressure P2 72 0 mm Hg Force chamber 20 2 mN e ZERO key This key is used to zero the force output If ON the icon is GREEN pH1 value 6 81 Probe temp 36 8 C Pressure setup menu rst Heat setup menu OE ONE Timer alarm SUE A einnsean cinched arctic eee ann Buzzer icon This icon indicates the status on the Buzzer If the icon is grey the Buzzer is OFF If the icon is green the Buzzer is ON active The Buzzer will make a sound when the Timer reaches zero OFF ON Timer The Timer is a countdown watch that can be set to a maximum of 24 hours 00 00 00 If the buzzer is activated it will sounds when the timer reached zero Heat icon This icon indicates the status of the chamber heat If the icon is grey Heat is OFF If the icon is green the Heat is ON active OFF ON Flow valve icon This icon indicate the status on the Flow valve If the icon is grey the Flow valve is OFF NO Flow If the icon is green the Flow valve is ON active OFF ON Pressure icon This icon indicates the status of the Pressure regulation If the icon is grey the Pressure regulation
11. ansducer P1 Pole DI Sakae Micropositioner for longitudinal adjustment of glass cannulas Figure 1 3 The Pressure Myograph Unit Fixation plate for left glass cannula e Fixation plate for right glass cannula 9 Outlet connecting pipe e perio e Perfusion inlet pipe 99 999 0000000000 Silicone tube connecting outlet connecting Silicone tube connecting perfusion inlet pipe and right pipe with left glass cannula glass cannula Left glass cannula e i A e Right glass cannula O Myo chamber window Figure 1 4 The Pressure Myograph chamber magnified 8 PRESSURE INTERFACE USER MANUAL CHAPTER 2 SETTING UP PRESSURE MYOGRAPH SYSTEM FlowMeter 161FM USB cable Waste Bottle USB cable Data Acquisition Software and PC dm a Peristaltic Pump USB cable optional Pressure Myograph 111P ss 4 incl Micr a Pressure Interface incl Microscope pH sensor optiona E C o Pressure and O Oxygen Supply RE SEIA pls optional Oxygen Supply Superfusion Sucction Bottle Buffer Outlet Superfusion buffer Inlet Superfusion buffer Inflow Perfusion buffer Outflow Perfusion buffer Cables Tubing Figure 2 1 Example of the Complete Pressure Myograph System Model 111P Fig 2 1 is a diagram of a complete setup for the Pressure Myograph System 111P The diagrams include optional equipment such as a peris
12. back panel of the Pressure Interface to an adjustable oxygen supply using the Atlas Copco Cablair hose delivered with the Pressure Myograph System IMPORTANT MAKE SURE THAT THE HOSE IS SECURELY FASTENED TO THE OXYGEN INLET USING THE HOSE CLIP THE PRESSURE FROM THE OXYGEN SUPPLY MUST NOT EXCEED 1 0 BAR 4 Microscope camera to PC connection Inside the DMT Microscope there is a USB camera wired to a USB cable and one USB cable to power the microscope light Connect the two USB cable from the DMT microscope to the computer USB ports For the 110P system with the external USB camera connect the USB cable from the USB Camera to your PC for the 110P system 5 Pressure Interface to external data acquisition system connection optional If you have not obtained the MyoVIEW Il software the four BNC ports on the rear of the Pressure Interface enable connection of an external data acquisition system for recording inlet pressure P1 outlet pressure P2 longitudinal force probe temperature chamber temperature flow ON OFF pressure ON OFF pH1 value and Optional Dig out1 Dig out2 Dig in1 dig in2 6 Turn on the power Turn the main power to the Pressure Interface ON at the power switch Turn ON the computer The Pressure Myograph System is now ready to be used with MyoVIEW Il Data Acquisition Software NOTE A DETAILED GUIDE TO CONNECTING THE PRESSURE MYOGRAPH SYSTEM FOR AN EXPERIMENT IS DESCRIBED IN DETAIL IN CHAPTER 5 For installation
13. d between 3900 to 4500 Apply 125 mmHg pressure on the Myo When the pressure reading is stable go to next step Pressure P1 4293 Pressure P2 4203 Pressure P3 4211 Step 4 Apply a pressure of 200mm Hg to the Pressure Myograph Chamber using the Big Ben Pressure Manometer When the 3 blue lines of relative PRESSURE CALIBRATION values stabilize go to NEXT STEP ser DO OB The relative values should between 4600 to 5200 Apply 200 mmHg pressure on the Myo When the pressure reading is stable go to next step Pressure P1 4905 Pressure P2 4788 Pressure P3 4 784 NEXT STEP lines are the actual pressure on all three pressure sensors The values must PRESSURE CALIBRATION Step 5 The calibration is now finished The readings in the three bottom be 200 0 1 0mm Hg Step no Ove If the readout is 200 0 1 0 mm Hg Carefully remove the Calibration Shunt Tube and reconnect the tubing to the calibration is OK the glass cannulas Otherwise repeat the calibration Pressure P1 200 0 mm Hg Pressure P2 200 0 mm Hg Pressure P3 200 0 mm Hg 24 PRESSURE INTERFACE USER MANUAL 3 6 3 pH Calibration Procedure optional Before the pH calibration is performed be sure to select the way the pH sensor is to be used See the sub menu pH SETUP under INTERFACE SETTINGS The temperature is an important parameter in the calibration formula and is obtained automatically if AUTO is sel
14. ected in Temperature compensation function shown below If MANUAL is selected the Manual temperature is used in the pH calibration formula and the temperature probe is de activated Inthe MANUAL mode the temperature of the calibration buffers is measured with a thermometer and entered manually in the manual temperature line Step 1 The pH calibration procedure is listed in 4 individual steps and needs to be performed one at a time Step 1 involves cleaning the pH probe and the temperature sensor with double distilled water When ready Press NEXT STEP Step 2 Place the pH meter electrode and temperature probe in the HIGH buffer solution here pH 7 and turn on stirring of the HIGH buffer solution When the relative pH output in the blue line is stable go to NEXT STEP Step 3 Place the pH meter electrode and temperature probe in the LOW buffer solution here pH 4 and turn on stirring of the LOW buffer solution When the relative pH output in the blue line is stable go to NEXT STEP Step 4 The calibration is now finished The values in the two bottom lines are the actual pH reading and the temperature reading pH SETUP MENU Low buffer pH 4 0 SELECT High buffer pH 7 0 SELECT Temperature comp AUTO SELECT Manual temp Value 20 C SELECT Step no ME Follow the pH calibration procedure in the User Manual Clean the pH probe and the temperature probe When ready go to next step
15. en the relative force reading is stable go to next step Force Chamber 3261 BACK NEXT STEP Step no GUI 5 Carefully place the 2 9 weight On the pan When the relative force reading is stable go to next step Force Chamber 3346 NEXT STEP FORCE CALIBRATION Step no UE Dw The transducer is now calibrated Force read out should be 9 81 mN 0 1 mN If OK go to next step Otherwise repeat the calibration NEXT STEP Force Chamber 9 81 mN CHAPTER 3 21 3 6 2 Pressure Calibration Procedure The setup to perform the pressure transducer calibration procedure is illustrated in Fig 3 3 Carefully follow the procedure described below to calibrate the Pressure Interface System The calibration procedure is listed in 5 individual steps and needs to be performed one at a time to calibrate the system Figure 3 3 Illustration of setup for pressure transducer calibration Step 1 involves setting up the Myograph Chamber for pressure calibration Step 2 Connect the Big Ben Pressure Manometer to the 3 way valve at P1 on the Pressure Myograph Chamber as illustrated in fig 3 3 Step 3 Inside the Myograph Chamber on the right hand side is a small stainless steel pipe which is connected to the right glass cannula via a small silicone tube Fig 3 4 B Carefully disconnect the silicone tube from the stainless steel pipe Step 4 Carefully disconnect the silicone tube between the outlet connecting pi
16. he TEMPERATURE DIFFERENCE function allows the user to fine tune the temperature setpoint of the system Although the temperature setpoint for the system can be set in the HEAT MENU the actual temperature for the system may not heat to the exact setpoint This function allows the user to adjust the temperature of the chamber and bottle individually so the EXACT temperatures can be reached This is referred to as a temperature offset TEMP OFFSET on chamber The SELECT and ALL functions are the same in this menu as previously described Pressing ENTER will save the values for future experiments RECORD 1 OUTPUT SET Record 1 Force Rec 1 out 2 5V 100mN SELECT Rec 1 out 2 5V 100mN SELECT INTERFACE SETTINGS TEMPERATURE DIFFERENCE FACTORY DIAGNOSTICS TEMP OFFSET Chamber 1 20 Bottle 1 1 C TEMP OFFSET Chamber 1 26 Bottle WASG CHAPTER 3 27 3 6 5 2 pH SETUP MENU Before the pH calibration is performed be sure to have selected the way the pH sensor is intended to be used This is done in the sub menu pH SETUP The temperature is an important parameter in the calibration formula and is obtained automatically if AUTO is selected in the Temperature compensation as shown below If MANUAL is chosen the Manual temperature is used in the pH calibration formula and the probe temperature is deactivated Inthe MANUAL mode the temperature of the calibration buffers is measured with a t
17. hermometer and entered manually in the manual temperature line 3 6 5 3 FACTORY DIAGNOSTICS Entering FACTORY DIAGNOSTICS will display the LOGIN CODE TO DIAGNOSTICS window This window is for trained technicians and is used for diagnostics and troubleshooting purposes The general user will not have access to this window Entering the proper 5 digit pin number however will give the trained technician access to Diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer 28 PRESSURE INTERFACE USER MANUAL pH SETUP MENU Low buffer pH 4 0 High buffer pH 7 0 Temperature comp AUTO Manual temp Value 20 C Type login code to get acces EM O 5 o Code init value 51761 BBBBB SELECT SELECT SELECT SELECT APPENDIX 1 SYSTEM SPECIFICATIONS TECHNICAL SPECIFICATIONS External 100 240VAC to 24VDC adapter Current max Dimensions LxDxH in cm Net weight Environmental humidity Environmental operating temperature Environmental storage temperature Fluid capacity One pH input range pH calibration 4 analog outputs Analog output range Output impedance Air connection USB connector TRANSDUCER DATA Maximum range Operating temperature Frequency range 10 3 3Amps at 24VDC 34 x25 x 15 5kg 20 to 80 RH non condensing 15 to 40 C 4 to 70 C 500 ml bottles We recom
18. is OFF If the icon is green the Pressure regulation is ON active OFF ON Zero key This key is used to zero the output from the force transducer Pressing ZERO this key will reset the baseline of the chart traces without affecting the calibrations CHAPTER3 15 3 3 Pressure menu The Pressure is controlled from this menu Use the SELECT key to select the line to be changed When selected the line change color is blue edit mode Use the DEFAULT button or the arrow keys to change the setpoint value Pressing ENTER will save the new setpoint P1 Inlet setpoint is the pressure value that the regulator is setting on the inlet side of the Myograph Pressure range is 0 250mmHg P2 Outlet setpoint is the pressure value that the regulator is setting on the outlet side of the Myograph Pressure range is 0 250mmHg Pressure gradient This sets the speed at which the regulator modifies the pressure The pressure change range is 1 10mmHg sec NOTE P1 MUST NEVER BE LOWER THAN P2 TO PREVENT BACKFLOW IN THE SYSTEM The Pressure regulator and the Flow are also controlled from this menu To turn the Pressure regulator ON press the Pressure ON button When activated the button changes to Green In the Main Menu the pressure icon also turns to green when pressure regulation is on ON To turn the Flow ON press the Flow ON button When activated the key changes to Green In the Main Menu the Flow icon also tur
19. may occur To start heating press HEAT ON Covering the chambers with the chamber covers will expedite the heating Place the temperature probe into the chamber for the first calibration to monitor when the chamber has reached the target temperature With the covers heating will take about 20 to 30 minutes for the chambers and transducers to reach 37 C Once the chamber s are heated and have reached the target temperature press NEXT STEP 20 PRESSURE INTERFACE USER MANUAL FORCE CALIBRATION Step no 8 2 5 Follow the weight calibration procedure in the User Manual Fill the chamber with double distilled water When ready go to next step NEXT STEP Step no JBC to 5 Place the calibration bridge on the myograph Place the tip of the Bridge behind the glass cannula Fixation plate When ready go to next step BACK NEXT STEP Step no 68 5 6 Turn the heat on Wait until the temperature is stable Temperature set point 37 0 C Probe temperature 36 8 C Step no OBULU Turn the heat on Wait until the temperature is stable Temperature set point 37 0 C Probe temperature 36 8 C BACK HEAT OFF NEXT STEP Step 4 This is the first step in the actual weight calibration process A 4 digit number will be displayed in blue at the bottom of the screen If nothing has been disturbed during the heating process the zero O gram calibration sh
20. mend a volume of 400ml to ensure optimal pressure regulation pH 0 14 manual with instructions on the display BNC connectors at rear to connect to external data recorder 2 5Volt lt 200 Ohm maximum pressure 1 Bar For downloading firmware and connection to PC 200MN 15 to 50 C O 20Hz APPENDIX 1 29 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China 21 5425 1330 Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
21. n a 8 Chapter 2 Setting up the Pressure Myograph System 1 211x 2ssssrsreaaeee ai eee iii 9 AL De Lume UPS Ed 10 2 2 Setting up the complete Pressure Myograph SYyStemM erre eee ereee rene eee re rene ee eee rene ene sas ererecoca nana rerecoca saca rerecoca saca rerenaca 10 2 9 The first w ight and pressure calibrations axis ga 10 2 4 External Pressure Myograph Unit connections cccccccccssesseeceecccesseecenccecesseeneuccecesseesenececessserenececeseeerenececcsssarenececesseasenesees 11 ZO OM Me P E a 11 2O EXTA Ret OCIS nn 12 2 1 Connecting superfusion flow optional siga iii Sa 12 2 8 Connecting the Flowmeter DMT 161FM optional ccsccccccssesseeeeecccesseesenccecesseesenececesseerenececesseerenececesssarenececesssetesesees 12 2 9 Connecting the oxygen SUPDPIY ai Sa 12 SUC ON E arene 13 2 11 3 Way Valve AUSENTE ais da 13 Chapter 3 The Pressure Interface menus 211ss arrese eran iii eee iii iii 14 3 1 General description of how to navigate the touch screen san icnicestinccasdzasessdnsvmecnccunerocceccedeostussaseasdenennciadcudcansuavaunumeraettentuctuaduseuative 14 ON SOFC accent
22. ns to green when Flow is on z ON 16 PRESSURE INTERFACE USER MANUAL PRESSURE SET P1 Inlet setpoint 120 0mmHg SELECT P2 Outlet setpoint 85 0mmHg SELECT Pressure gradient 5 0mmHg sec SELECT Pressure Corr DEFAULT PRESSURE SET P1 Inlet setpoint 120 0mmHg P2 Outlet setpoint 85 0mmHg 5 0mmHg sec Pressure Corr o 3 DEFAULT Pressure gradient PRESSURE SET P1 Inlet setpoint 120 0mmHg SELECT P2 Outlet setpoint 85 0mmHg SELECT Pressure gradient Pressure DEFAULT PRESSURE SET P1 Inlet setpoint 120 0mmHg P2 Outlet setpoint 85 0mmHg 5 0mmHg sec Pressure gradient Pressure DEFAULT 3 4 Heat Menu The Chamber and Bottle heating temperature are controlled from this menu SET CHAMBER TEMPERATURE To turn the heat on or to change the temperature for the system press the HEAT button in the Main Menu The display will enter the Heat menu and allow the user to change the system temperature as well as turn heat on or Probe temperature 36 6 C off Pressing DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing the up or down arrows Pressing ENTER will save the new temperature setpoint Temperature setpoint 37 0 C LIMES To turn the heat on touch ON and the ON button will turn green The system will heat to the designated temperature setpoint SET CHAMBER TEMPERATURE Temperature
23. oVIEW Il software program optional e Computer optional FlowMeter Model 161FM optional e User manual FlowMeter Model DMT 161FM e MyoFlow FlowMeter software including drivers CD ROM Peristaltic pump for superfusion flow optional e Watson Marlow peristaltic pump 6 PRESSURE INTERFACE USER MANUAL CHAPTER 1 SYSTEM OVERVIEW 1 1 Pressure Interface i gt aaa i LLELE L A gt SS eas e 2x 500 ml Schott Duran bottles Pressure Interface touch display Power indicator Figure 1 1 The Pressure Interface front panel ON OFF switch e Earth point Inlet for oxygen car bogen to pressurize bottles max 1 bar 24V DC port 9 for power Connection port for RR EENE TA Pressure Myograph unit USB connection port for computer E e Connection port for pH meter optional Analogue recorder output connection to external data acquisition software optional Figure 1 2 The Pressure Interface rear panel CHAPTER 1 7 1 2 Pressure Myograph Unit Horizontal adjustment screw 9 Micropositioner for vertical adjustment of glass cannulas Force transducer Outlet pressure transducer 25 pin serial port for communication with connection pipe Pressure Interface Inlet 3 way valve 6 Outlet 3 way valve e Outlet pressure transducer P2 Inlet pressure tr
24. of MyoVIEW Il refer to the MyoVIEW II Quick Installation Guide 2 3 The first weight and pressure calibrations Prior to shipment the Pressure Myograph System has gone through two days of continuous testing including final weight and pressure calibrations However in order to ensure that the Myograph is working at highest performance DMT recommends that new weight and pressure calibrations are performed before the first use The weight and pressure calibration procedures are described in detail in chapter 3 6 1 and 3 6 2 10 PRESSURE INTERFACE USER MANUAL 2 4 External Pressure Myograph Unit connections This section illustrate how to connect the perfusion circuit the superfusion circuit oxygen supply pressure supply and suction device on to the Pressure Myograph unit Suction pipe to vacuum Perfusion inlet 1 Perfusion outlet P2 Extra perfusion outlet e e Superfusion inlet silas Pipe for oxygen supply eee Access hole for reagents buffer temperature or pH probe Figure 2 2 External Pressure Myograph connection 2 5 Connecting perfusion flow The perfusion flow is controlled by the Pressure Interface Instructions on how to control the perfusion flow are provided in chapter 3 To connect the Pressure Interface to the Pressure Myograph unit use the silicone tube internal diameter 1mm delivered with the Pressure Myograph System e Connect the perfusion inlet at P1 on the Pressure
25. or further information about recommended perfusion pump models specifications and prices The superfusion circuit is connected to the Pressure Myograph Unit as illustrated in fig 2 1 2 8 Connecting the Flowmeter DMT 161FM optional The DMT Flowmeter Model 161FM is optional for flow measurements in the range of 15 4000ul min The Flowmeter is easily combined with any DMT Pressure Myograph System as illustrated in fig 2 1 Use of the Flowmeter permits control of intravascular pressure pressure gradient and flow conditions with high accuracy This enables in vitro studies on vessel segments under conditions very close to those found in vivo Adding the DMT FlowMeter DMT 161FM to the Pressure System also enables measurement of flow rate as a parameter The Flowmeter data will make it possible to record live traces of the flow dependent parameters as shear stress Reynolds number and vascular resistance due to changes in vessel structure and function in various physiological and pharmacological studies To add the FlowMeter DMT161FM to the Pressure System the cable from the flow is attached to the Flowmeter port marked on the backside of the Pressure Interface The Software MyoVIEW Il will recognize the Flowmeter DMT161 as soon as it is connected to the Pressure Interface and will be ready for use immediately Please consult the MyoVIEW II video manuals linked in the help menu of MyoVIEW II 2 9 Connecting the oxygen supply Connect
26. ould be stable The 4 digit number will not fluctuate and after 30 45 seconds NEXT STEP can be pressed If the 4 digit number is not stable wait before pressing NEXT STEP Step 5 This step is the 2 gram weight calibration At this step place the 2 gram weight in the pan closest to the transducer over the transducer to simulate the stretch created by the contraction of a mounted muscle preparation Remember a 2 gram weight in 8 90 vector is divided and the transducer will only detect 1 gram or 9 81 mN of force The weight placement should cause a positive increase in the 4 digit number Wait at least 30 to 45 seconds for the applied force to stabilize before pressing NEXT STEP Only when the 4 digit number has stabilized should you press NEXT STEP Step 6 This step verifies that the calibration was performed correctly The Force Chamber reading should be 9 81 0 1 mN If the Force Chamber reading is off by more than 0 1 mN then remove the weight press BACK to return to Step 4 and repeat the calibration process If the Force Chamber reading is satisfactory then press NEXT STEP to end the calibration After calibrating the force transducer carefully remove weight balance and calibration bridge The Pressure Myograph System is now ready for longitudinal force measurements FORCE CALIBRATION sere OVO0BOO Make sure that the transducer is not subjected to any force Wh
27. pe and the outlet pressure transducer connecting pipe fig 3 4 A Step 5 Close the inlet from the Pressure Interface to P1 by turning the handle on the 3 way valve towards the normal inlet way The inlet to P1 now comes from the pressure manometer See fig 3 3 22 PRESSURE INTERFACE USER MANUAL Figure 3 4 Disconnection of silicone tubes Figure 3 5 Mounting of Resistance tube shunt CHAPTER3 23 3 6 2 1 Pressure calibration PRESSURE CALIBRATION Press setting in the Main Menu and then press Pressure Calibration sen DODDO Step 1 Press NEXT STEP when the system is ready Follow the Pressure calibration Procedure in the User Manual Prepare the Myo Chamber for Calibration When ready go to next step NEXT STEP Step 2 Apply a pressure of 50mm Hg on the Pressure Myograph using the PRESSURE CALIBRATION Big Ben Pressure Manometer When the 3 blue lines of relative values stabilize go to NEXT STEP se 09000 The relative values should between 3300 to 3900 Apply 50 mmHg pressure on the Myo When the pressure reading is stable go to next step Pressure P1 3672 Pressure P2 3615 Pressure P3 3676 BACK NEXT STEP Step 3 Apply a pressure of 125mm Hg to the Pressure Myograph Chamber using the Big Ben Pressure Manometer When the 3 blue lines of relative PRESSURE CALIBRATION values stabilize go to NEXT STEP Step no BO The relative values shoul
28. setpoint 37 0 C In the Main Menu the thermometer icon turns to green when the heat is on Probe temperature 36 6 C IMM 3 5 Timer Alarm Menu SET TIMER The Timer and Buzzer Alarm are controlled from this menu Set Timer 00 00 00 Use SELECT to program the timer The timer can be programmed in intervals from 0 to 23 59 59 Hours minutes seconds When Set Timer Timer o is selected use the up and down arrows to program the timer The hours are programmed first and then use the RIGHT button to get to the minutes Buzz Alarm o and then the seconds WIE Pressing ENTER will save the programmed time SET TIMER Set Timer 00 00 00 Set Timer 00 00 00 Set Timer 00 00 00 To start the Timer press the Timer ON button When the Timer is on the button turns green When the timer reached zero it switches automatically SetTimer 00 00 00 to OFF gt If the Buzzer is set ON it will make a tone when the timer reaches zero Buzz Alarm CHAPTERS 17 activate the Buzzer Alarm press the Buzzer Alarm ON button When ON the button turns green and in the Main Menu the bell icon also turns SET TIMER green Set Timer 00 00 00 O ON Buzz Alarm on 3 6 Settings Menu The Settings Menu contains several sub menus These sub menus SETTINGS include PRESSURE CALIBRATION pH CALIBRATION optional RECORD OUT SELECT INTERF
29. tact us immediately and the matter will be pursued as soon as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Pressure Myograph System In case of transport and the original packing material is unavailable please contact the DMT Sales Department for advice and packing instructions After unpacking your new Pressure Myograph System please use the following list to check that the system is complete Pressure Myograph Unit e Force calibration kit including bridge balance and 2 gram weight e Resistance tube shunt calibration tube e Two glass cannulas e Chamber cover Pressure Interface e Pressure Interface to chamber cable with a temperature probe e Power adapter the shape of the AC plug varies by country be sure that the plug has the right shape for your location e USB cables for PC connection e Atlas Copco Cablair hose for main pressure supply e Two 500m Schott Duran Bottles with specially designed screw caps Riester Big Ben Pressure Manometer Accessories e Allen key e Spare screws e Small screwdriver e Suture wire e Tube of high vacuum grease e Tube of grease for linear slides e Silicone tubing internal diameter Imm Computer hardware software amp manuals e USB digital camera optional e CD with DMT Pressure Myograph Systems user manuals e CD with My
30. taltic pump to enable superfusion flow and pH probes for measuring pH in the superfusion buffer Also the diagram includes the DMT Flowmeter DMT 161FM The Flowmeter enables flow measurements in the range of 15 4000ul min Fig 2 1 is intended as an overview of the setup of the Pressure Myograph Systems A detailed step by step description is provided in the remaining sections of Chapter 2 The Pressure Myograph System will work with a range of different microscopes the DMT Microscope Zeiss Axio Vert A1 Nikon TS 100F Motic AE2000 or your own inverted microscope of choice NOTE THE MICROSCOPE IS ESSENTIAL FOR THE DIMENSIONAL ANALYSIS OF THE MOUNTED VESSELS The DMT Microscope is a standard inverted microscope with a built in digital CCD USB camera The DMT Microscope is very effective and recommended in setups requiring standard image analysis The Zeiss Axio vert A1 is an inverted microscope that provides the highest degree of flexibility in image analysis The Zeiss Axio vert A1 a Nikon TS 100F and Motic AE 2000 enable the use of a wide range of objectives and facilitate sophisticated fluorescence techniques Contact the DMT Sales Department for further product information and prices on any optional equipment including microscopes CHAPTER2 9 2 1 Setting up step by step NOTE IF YOU HAVE PURCHASED A COMPUTER FROM DMT IN CONJUNCTION WITH YOUR PRESSURE MYOGRAPH SYSTEM THEN THE MYOVIEW Il DATA ACQUISITION SOFTWARE HAS ALREADY BEEN INST
31. ting that the interface is in edit mode and waiting for input When DEFAULT is chosen a default value will be PRESSURE SET displayed To change the numeric value for the selected parameter touch the up or down arrow keys P1 Inlet setpoint 120 0mmHg SELECT P2 Outlet setpoint 85 0mmHg SELECT Pressure gradient 5 0mmHg sec SELECT Pressure FIOW DEFAULT Once the desired setting has been chosen pressing ENTER will save the selection and the new value will be stored in memory The selected line will PRESSURE SET revert to black Pressing the white X in the red box will exit the menu and return you to the Main Menu P1 Inlet setpoint 120 0mmHg SELECT P2 Outlet setpoint 85 0mmHg SELECT Pressure gradient 5 0mmHg sec SELECT Pressure Flow MULTI INTERFACE Multi Myograph System Model 110P 111P Software Revision 01 02 Date Nov 27 2011 3 1 Power up screen After turning on the Pressure Interface an Introduction screen appears It displays the software versions number While this screen is displayed the system is auto initialising After a few seconds the MAIN MENU display will appear 3 2 Main Menu The Main Menu gives a good overall picture of how the Pressure Interface is working It displays values for the input pressure output pressure force pH probe temperature and time and the status of systems including the time heat flow and pressure 14 PRESSURE INTERFACE US
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