B - GE Healthcare Life Sciences
Contents
1. 1 Select main menu Setup press OK 2 Select sub menu Set Display Angle press OK 3 Select viewing angle gt Up gt Mid or gt Down press OK B 3 Alarm timer You can set the alarm function to either a fixed alarm time or using a count down timer The pump can be started or stopped automatically or an alarm can sound at the set time It is not possible to set both an alarm time and a count down timer Current values are shown in parentheses 1 Select main menu Alarm Timer press OK 2 Set the action to take place Press OK to select action Buzzer will generate an audible alarm for 15 s and a message Run will start the pump at the set flow rate End will stop the pump each generating one beep and a message 3 Use the sub menu Set Alarm if you want to set an alarm at a fixed time Press OK to enter the time value in the form HH MM SS pressing the OK button after entering each time unit 4 If you want to set a count down timer turn the dial to select sub menu Set Timer Press OK to enter the countdown value in the form HH MM SS pressing the OK button after entering each time unit 5 Press ESC button to return to the Alarm Timer menu which now shows the set alarm time or count down time as BzZHH MM sS 6 When the alarm time is due or the count down timer reaches 00 00 00 an alert display is shown and the instrument beeps until the OK button is pressed AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition A2. 6 Repeat steps 3 to 5 for pump module B if fitted 7 Check that the outlet tubings are not blocked 8 Connect a thin capillary or a column that will give sufficient back pressure 9 Run at the following flow rates for 15 minutes P 901 1 ml min or 2 ml min 50 B P 903 0 1 ml min or 0 2 ml min 50 B 10a For P 901 run at 20 ml min or 40 ml min 50 B at a backpressure of 2 5 MPa for 15 minutes 10b For P 903 run at 2 ml min or 4 ml min 50 B at a backpressure of 5 10 MPa for 2 hours or longer if possible e g overnight 11 Finally change the eluent according to the description given in section 3 9 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 33 Maintenance 4 6 Replacing a damaged piston Typical symptoms of a damaged piston are observed as excessive piston seal wear unstable pressure a reduction in the flow or in some cases noise as the piston moves The piston should be removed examined for damage or salt precipitation and then replaced with a new piston if necessary If a damaged piston has been in operation the piston seal will be destroyed and should also be replaced To replace the piston and the seal follow the instructions in section 4 4 Replacing the piston seal In addition to the spare parts listed in section 4 4 the following are required see Reference information D for code no P 901 Piston kit 100 ml or P 903 Piston kit 10 ml 4 7 Cleaning the inlet and outlet check valves
3. AE 19 Installation 20 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Operation 3 Operation Selftest 3 1 On off Switch on the instrument at the mains switch on the rear panel At switch on the instrument performs a selftest and then asks for synchronization Pump P 900 Open the purge valves see section 2 8 to avoid high pressure during synchronisation which is done at the speed of 0 1 ml min and press OK The display will show Synchronizing pumps and after approx 1 minute the display shows the main operating menu with the pump in End mode Close Pe o ME the purge valves All parameters are factory set to default values Pump synchronization 3 2 Menu selection and settings 3 2 1 Menu selection A specific menu is selected by turning the front selection dial clockwise or counterclockwise When the required menu is visible the menu or selection is accepted by pressing the OK button Menu selection OK button ESC button Select submenu Return one menu level If a menu has sub levels the sub menu is displayed by pressing the OK button Pressing the ESC button moves back one menu level Main menus Sub menus Sub menus Mai 2 gt k gt OK Sub menu 2 1 Sub menu 2 1 1 ESC ESC Sub menu 2 2 ESC Sub menu 2 3 2 KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 21 Operation 3 2 2 Return to main menu Pressing
4. Check Heads 23 0MPa Check Total Run Time Check Piston Strokes Check Service Mode Telephone Service 012345678901 Calib Press Offset Contract Number 012345678901 Serial Number 01234567 Pump P 900 v1 00 Date of Maintenance Setup Setup Hi Press Limit j Buzzer Test Setup Lo Press Limit Setup Pr Full Scale Setup Pressure Unit Setup Gradient Base tin Setup Gradient Mode Setup Pump A Type Setup Pump B Type Setup Compr comp of off on Setup Comp A 4 0E 10 4 0 Setup Comp B Alarm Timer 12 30 52 Alarm Timer action 4 Fi Bzz Buzzer Run End Setup Mixer on Aaa Setup Valve 1 Set Timer 0 00 00 00 Setup language 00 36 53 00 36 53 Seu Cols oume i 2 S EE Set Display Angle Ss AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 53 B Reference information 54 KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE C Technical specifications Reference information C 1 Operating data The specifications are valid only at pressures higher than 0 2 MPa Flow rate range P 901 isocratic mode gradient mode double mode P 903 isocratic mode gradient mode double mode Pressure range P 901 P 903 pH stability range Viscosity Flow rate accuracy P 901 0 2 10 0 MPa P 903 0 2 25 0 MPa Flow rate reproducibility P 901 Flow rate 20 5 ml min P 903 Flow rate 20 05 ml min Gradient composition P 901 accuracy reproducibility P 903 accuracy reproducibility Pressure s
5. Faulty operation of the check valves is usually indicated by irregular flow very low flow or unstable pressure traces Probable causes of this are air or dirt in a check valve preventing it from closing to seal and hold the pressure Record the pressure see section 5 4 and identify the faulty check valve by observing which pump head is delivering the flow see section B 1 1 First try to clean the check valves in place on the pump head by pumping 100 methanol for approximately 10 minutes If this does not correct the problem follow the instructions for removing and then cleaning the valves Note Change solvent to distilled water and flush out all salt before removingthe check valves Tools required A 13 mm and a 1 4 inch wrench gt 4 7 1 Cleaning after removal Before removing the check valves move all input buffers bottles below the level of the pump heads to prevent siphoning 1 If the condition of the check valve is not improved by in place cleaning disconnect and remove the inlet manifold and outlet tubing 2 Use the 13 mm wrench to remove the valve from the pump head CAUTION Handle the check valves with care when they have been removed from the pump heads to prevent loss of any internal components 34 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Maintenance 3 Immerse the complete valve in methanol and place in an ultrasonic bath for some minutes Then repeat the ultrasonic bat
6. MPa 10 MPa e Large leakage in one pump head 5s e Large leakage in one inlet check valve e Inlet open or blocked 5 5 Removing trapped air bubbles from the pump During routine operation the presence of air bubbles in the pump heads is seen as an erratic flow a noisy detector signal or an irregular pressure recording With air bubbles trapped in the pump the pressure reading on the display will fluctuate considerably To remove the air from the pump 1 If the air has accumulated because of a leaking connector correct the fault 2 Ensure that the inlet filter is fully immersed in eluent 3 Purge the pump according to section 2 8 Purging the pump If the problem remains try to remove the air bubbles in accordance with the method described below Use 100 methanol 1 Run at 40 ml min for P 901 or 4 ml min for P 903 2 Continue to run for 10 minutes or until the air bubble is removed 3 Change the eluent according to the description given in section 3 9 40 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information Ce A Description A 1 Instrument Pump P 900 is a high performance laboratory pump for use in liquid chromatography and other applications where accurately controlled liquid flow is required An eluent in an external vessel is drawn into the inlet manifold by the action of the pump Twin reciprocating pump heads work in unison to deliver a s
7. V1 00 Date of Maintenance Buzzer Test B 1 Check menu B 1 1 Checking piston stroke To enable trouble shooting it is possible to check which pump module head that delivers flow 1 Select main menu Check press OK 2 The display shows the status of the pistons for both pump modules A Left means that the left pump head is delivering flow in the A pump and B Right the right pump head in the B pump At the changing point both are displayed The A pump is closest to the front panel B 1 2 Check pump operation time 1 Select main menu Check press OK 2 Select sub menu Check Total Run Time The display shows the accumulated operation time Run and Hold mode for the A and B pumps B 1 3 Check number of piston strokes 1 Select main menu Check press OK 2 Select sub menu Check Piston Strokes The display shows the accumulated number of piston strokes for the A and B pumps B 1 4 Check Service Mode Service information relevant to the instrument can be checked Information may not be available in all menus 1 Select main menu Check press OK 2 Select sub menu Check Service Mode press OK 3 The service telephone number is displayed press OK 4 The service contract number is displayed press OK 5 The instrument serial number is displayed press OK 6 Instrument name and software version are displayed press OK 7 The date of the last service is displayed press OK 8 Atest of the instrumen
8. chemicals used Follow local regulations and instructions for safe operation and maintenance of the system WARNING This is a Class A product In domestic environment this product may cause radio interference in which the user may be required to take adequate measures gt Pee gt gt gt KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 11 Introduction 12 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Installation 2 Installation 2 1 Unpacking Unpack the instrument and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transportation It is recommended that all packing materials should be retained if onward transport of the instrument is expected CAUTION The following information should be read carefully to ensure that the instrument is installed correctly 2 2 General precautions The instrument should be installed in a non corrosive atmosphere The instrument should be located in a place of low temperature variations away from heat sources draughts and direct sunlight The instrument may be operated at normal ambient temperatures in the range 4 to 40 C The instrument should be installed on a stable laboratory bench providing a suitable working area or in an AKTA system To ensure correct ventilation a free space of 0 1 m is required behind and in front of the instrument Do not
9. controlled from a PC running UNICORN version 2 10 or higher using UniNet cables CAUTION The mains power to AKTAexplorer and AKTApurifier must be switched OFF before connecting the instrument to the UniNet 1 link 1 Connect two UniNet cables to the UniNet 1 connectors The instrument can be connected in series anywhere in the chain between the PC and the termination plug The UniNet 1 link connects in series the PC with Pump P 900 and other instruments The termination plug is connected to the last instrument in the chain 2 5 Connecting to supply voltage 1 Make sure the on off switch is in the OFF position O 2 Connect a mains cable between the instrument and a grounded mains socket The instrument is delivered with both European and US type mains cables as standard Any voltage from 100 240 V AC 50 60 Hz can be used WARNING The instrument must be connected to a grounded mains socket The instrument contains no user replaceable fuse WARNING Only use mains cables delivered or approved by GE Healthcare AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Installation 2 6 Installing the piston seal rinsing system Note For the protection of the new pump do not run the pump until the system has been flushed out and primed with eluent according to sections 2 8 and 2 9 The rinsing system for the piston seals must be installed before use 20 ethanol should be passed around the pisto
10. head unless there is good reason to believe that the seal is leaking Always ensure that sufficient spare components are available before attempting to replace the piston seal It is not possible to reinstall a used piston seal after removal Note The power must be switched OFF when removing and refitting the pump heads Note Always replace the piston seals on both pump heads at the same time An even better practice is to replace all four piston seals Spare parts and tools required Seal kit containing see Reference information Dfor code no 2 or 4 piston seals 2 or 4 rinse membranes 1 4 inch wrench supplied with the pump 3 mm allen key supplied with the pump Screwdriver supplied with the pump AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Maintenance Note After a new seal has been installed the pump should be run in see section 4 5 Running in a new piston seal Note Before disassembling the pump heads move all input buffers bottles below the level of the pump heads to prevent siphoning CAUTION Read the following instructions carefully The individual parts of the pump head can be assembled incorrectly Take care to ensure that the orientation of each part is correct before continuing with the next instruction 1 Switch off the pump at the mains power switch on the back panel Remove the piston seal rinsing system The connectors are simple plug in fittings 2 Completely
11. running when the pump is in Run Pause or Hold mode B 2 9 Set valve operation Sets the condition of any switch valve SV 903 connected to the pump 1 Select sub menu Setup Valve 1 press OK Setup Valve 1 2 Select either off or on press OK IN IN NC L NO NC A NO INA CTIVE ACTIVE B 2 10 Setup language Sets the language used in the display 1 Select sub menu Setup Language press OK Setup language GB GB D F E I 2 Select the desired language GB British English D German F French E Spanish Italian 50 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Setup Unit Number Set Display Angle Alarm Timer 12 30 52 Alarm Timer action Bzz Buzzer Run End Set Alarm 12 32 21 0 00 00 00 Set Timer 0 00 00 00 Alarm Timer 12 30 52 Bzz12 33 00 Bzz12 41 29 12 41 49 Alarm time Reference information CE B 2 11 Setup unit number The unit number is the identification the pump has on the UniNetbus It should correspond to the number set in UNICORN for the pump The number should be set to 0 if one pump is used If more than one pump is used they must all have different identification numbers 1 Select sub menu Setup Unit Number press OK 2 Select unit number 0 25 press OK B 2 12 Setup display angle The display angle can be set to compensate for different viewing heights
12. snnersnernnnr ser snar snnernner non nannr ennen nn B 2 9 Set valve Operation ammsmsmmmsemssnssssrssesnesnssossrsssnsnernnsnnsnransnnsnsnnsnnarannannnnennnn B 2 10 Setup IGA GUAGE vassa inteasr trias iielssinss iii B 2 11 Setup unit number 3 B 2 12 S t up display GAGE sascscsschcscccssscadedancdisdaastsabestb cist ebtashcschanssnstaotoDasbetannis B3 ACMI N anni B4 Service CISPICGYS eeeeeeessetssssssssssessseesseeceeeceeesceessnesnssseunsuenstseeeeesee B S Menu OVErVIGW ceeeesssssccccsesssnseescccccceesnsnseessececcessnsnsssececeseessnsnsesseeete C Technical specifications C 1 Operating data s ssmmmmmmmmsmsermmemnmsmmrsrmsesnnsn 53 C2 Physical data D Accessories and spare parts 6 Short instructions About this manual This manual comprises two parts a practical part sections 1 5 and a reference part sections A D Sections 1 5 contain the necessary information for operating the instrument AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 7 Contents 8 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Introduction 1 Introduction 1 1 General Pump P 900 is the collective name for a pump family The family members are e P 901 stand alone or system mounted Used as stand alone or in AKTA explorer 100 Equipped with four pump heads in two pump modules e P 903 stand alone or system mounted Used as stand alone or in KTA purifier 10 10 XT and AKTAexplore
13. the action of the piston being withdrawn from the pump chamber Outlet check valves Outlet manifold Outlet to mixer Outlet check valve SE Rinse chamber Inlet manifold Inlet Inlet check valve AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 43 A Reference information Piston Drain plate Rinse membrane Rinse chamber outlet Outlet check valve P 901 44 5 Return spring On the delivery stroke of the piston the inlet valve is sealed by the pressure developed and eluent is forced out through a similar check valve at the outlet Piston Return spring Drainage hole Drainage hole a Drain plate Rinse membrane Rinse chamber Rinse chamber Rinse chamber 2 outlet Cp Support washer Inlet Inlet check valve Jointing ring check valve Piston seal Outlet check valve P 903 The pistons are actuated by cams eccentrics driven by the motors Force for the retraction of the pistons is provided by coil springs The length of stroke of the pistons is fixed and changes in the flow rate are made by varying the speed of the drive motor Leakage between the pump chamber and the drive mechanism is prevented by a piston seal The pistons and seals are continuously lubricated by the presence of eluent To prevent any deposition of salts from aqueous eluents on the piston and to prolong the life of the seals the low pre
14. 00 567 1008 e Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45 16 2424 e Finland amp Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 e France Tel 01 69 35 67 00 Fax 01 69 41 96 77 Germany Tel 0761 4903 490 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 e Norway Tel 815 65 555 Fax 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 e Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 e USA Tel 800 526 3593 Fax 877 295 8102 imagination at work E Elanders Tofters 2005 User Manual 18 1120 04 AE
15. 1112 54 PEEK tubing i d 1 0 mm o d 1 16 2m 18 1115 83 Fingertight connector 1 16 10 18 1112 55 60 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Short instructions Tel 12 Short instructions Selftest Pump synchronization End 0 00mI min 0 00MPa 0 0 B Set Flow Rate End 2 50 ml min Run 2 50 ml min 2 0MPa 0 0 B Set Gradient Length Set Gradient Target Run 2 50mlI min The following short instructions are intended as a guide to users who are fully familiar with safety precautions and operating instructions described in this manual The instructions assumes that the instrument is installed according to the installation instructions 1 Switch on the instrument by means of the mains switch on the rear panel 2 Press OK to start synchronisation 3 The main operating menu is shown 4 Set the flow rate 5 Start the pump by setting it in RUN mode 6 The main operating menu is shown 7 Start a gradient by setting the Gradient Length and Gradient Target 8 Stop the pump by setting it in END mode AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 61 www chromatography amershambiosciences com www gehealthcare com GE Healthcare Bio sciences AB Bj rkgatan 30 751 84 Uppsala Sweeden Drop Design FPLC Superloop UNICORN and AKTA are trademarks of GE Healthcare Ltd a General Electric Company GE ima
16. 2 10 or higher These accessories are e Mixer M 925 e 2 way Switching Valve SV 903 KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Introduction 12 Safety IMPORTANT Pump P 900 is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes e The instrument is designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water e Do not block the air inlet and outlet of the unit WARNING Always disconnect the power supply before attempting to replace any item on the instrument WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are in use Connect the tubing by first inserting the tubing fully then tightening the connector fingertight Finally tighten the connector a further 1 4 turn using the key supplied WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING The instrument must not be opened by the user It contains high voltage circuits which can give a lethal electric shock WARNING The instrument must be connected to a grounded mains socket WARNING NaOH is injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the
17. 2 2 No 61010 1 e UL61010 1 EMC standards This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 Class A emission e This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 57 Reference information 58 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information D D Accessories and spare parts Item Quantity per pack Code no P 901 Stand alone 1 18 1114 00 P 901 System mounted 1 18 1108 56 Seal kit in4cluding 4 piston seals and 4 rinse membranes Seal kit 100 ml 1 18 1113 12 Piston kit 100 ml 1 18 1112 13 Pump head capillaries 100 ml 1 18 1117 52 Rinsing draining housing 100 ml 1 18 1112 03 Pump head 100 ml complete 1 18 1128 48 nlet manifold 100 ml UNF 5 16 1 18 1112 25 P 903 Stand alone 1 18 3000 00 P 903 System mounted 1 18 3100 00 Seal kit including 4 piston seals and 4 rinse membranes Seal kit 10 ml 1 18 1120 77 Piston kit 10 ml 1 18 1120 75 Pump head capillaries 10 ml 1 18 1120 81 Rinsing draining housing 10 ml 1 18 1120 76 Pump head 10 ml complet
18. E 51 B Reference information Set Clock 00 36 53 00 36 53 Alarm Timer off Buzz 05 15 00 Enter Access Code 52 The alarm timer is based on the internal instrument clock which can be set in the Set Clock menu placed after the Alarm Timer menu The clock will be reset when power is turned OFF An already set alarm timer function can be reset by pressing OK in the menu Alarm Timer off B 4 Service displays The instrument has service displays for use by authorised service personnel If the service display Enter Access Code is accidentally selected press the ESC button to exit to the normal operation display KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information CE B 5 Menu overview End 1 00mI min Run 1 00mI min Pause 1 00ml min Run 23 40ml min Hold 1 00ml min 2 00MPa 45 5 B Set Flow Rate Set Conc Gradient Set Concentration B Set Gradient Length Set Gradient Target Check
19. ESC repeatedly always returns to the main menu 2 which is the main operating menu Press ESC once more to return to main menu 1 the mode changing menu nn T 3 2 3 Select value Main menu 1 A cursor below a text or numerical value shows what is affected by the dial To increase the value turn the dial clockwise To decrease the value turn the dial counterclockwise The value can be reset by turning the dial several clicks counterclockwise Parameter set Hi Press Limit 10 00MPa 50 0 Current value oo ES a New value to be set When setting numerical values the cursor moves up to the next digit if the dial is turned quickly in one direction to simplify entering large values The cursor moves back one place to the right every two seconds if the dial is not turned The text or numerical value displayed is accepted by pressing the K button To cancel press the ESC button 3 3 Main menu overview Mode changing menu This is from where the pump is started and stopped The menu is accessed from all positions by pressing the ESC button repeatedly The appearance of this menu will depent on the current mode End 1 00ml min Main operating menu The menu is accessed from all positions by pressing 2 00MPa 45 5 B the ESC button repeatedly Run 23 40ml min Bet how Rate Setting flow rate in ml min Set Conc Gradient Setting concentration and gradie
20. GE Healthcare AKTAdesign Pump P 900 User Manual eD KTA Important user information All users must read this entire manual to fully understand the safe use of the component WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symobl indicates that the waste of electrical FX and electronic equipment must not be disposed as unsorted municipal waste and must be mmm collected separately Please c
21. Liquid leaking between the Piston seal or rinsing membrane incorrectly fitted or worn pump head and the 1 Replace or re install the seal or membrane side panel 2 Run in carefully see section 4 5 Running in a new piston seal AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 37 Trouble shooting SS Fault Action Low eluent flow and noise 1 Disassemble pump head and examine the as the pistons move piston spring according to section 4 4 Replacing the piston seal Replace if necessary 2 If spring is corroded check piston seal and rinse membrane Ensure that piston rinsing system is always used when working with aqueous buffers with high salt concentration 3 Check the piston for damage If damaged replace the piston according to section 4 6 Replacing a damaged piston 4 Remember to replace the piston seal and rinse membrane with new items Leaking connection and or 1 Unscrew the connector and check if it is worn crystalized material around or incorrectly fitted If so replace the connector a connector 2 Gently tighten the connector with your fingers Error in external chart recorder 1 Check the chart recorder in accordance with its manual 5 3 Error messages If the suggested actions do not correct the fault call GE Healthcare Message Action ERROR No mixer 1 Switch off the instrument Check connection 2 Check the connections to the mixer ERROR in mixer 3 Switch on the instrument Check mixer ERROR
22. NaOH and distilled water before service and maintenance gt gt gt KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 27 Maintenance 28 4 3 General care Inspect the complete system daily for eluent leaks e Wipe the surface regularly with a damp cloth Do not allow spilt liquid to dry on the instrument e Remove dirt from the surface using a cloth and a mild cleaning agent e Let the system dry completely before using it If at any time air is observed in the inlet tubing purge the pump by opening the appropriate purge valve and running the pump at max flow rate for about 1 minute If there is a lot of air in the inlet tubing stop the pump and perform the procedure in section 2 8 Purging the pump 4 3 1 General recommendations for all eluents It is essential that all liquids passing through the pump are clean and pure Impure or dirty eluents will not only cause baseline noise and drift but will cause damage to the pump head assemblies 4 3 2 Additional recommendations for aqueous eluents After running with an aqueous eluent the pump should always be thoroughly purged with pure distilled water to prevent salt precipitation 4 4 Replacing the piston seal If there are signs of liquid leaking between the pump head and the housing side panel or the volume of the rinsing solution has increased or decreased replace the piston seal of the leaking pump head CAUTION Do not disassemble the pump
23. PUMP smmmssmmmmemmsm 21 3 4 1 Main operating MENU h ssssssssssssssssssessssccsssssssessseccessssnseeeseceesssnnieeeeseeesessnees 21 3 4 2 Mode CHANGING MENU samernas 21 3 4 3 Preparation before starting m s ssmmmmmssmmsmsssmmss 21 344 EMErDeNCU StO Dassssssdessisidsssmeasersssensnotessdns esiiprrensirsssstnsnssidrntenssntrsednens inn 22 3 5 Setting the flow rate and starting the PUMP 22 3 6 Setting COMCEMTFATION B csssescsssssssssssnsesesssssssssnanssssscessesesnenseseecs 22 3 7 RUNNING a simple gradieNt s msmmmmmmmsmsmsmsmmm 23 3 8 Ending the run and Storage 111 i4 msssmsismsussvusvsvsuva 23 3 9 Changing Cl Ue Hts sasascissdesssssecseccscceccnussvssestsicaddisvaeswencateessmneevnscccen 3 10 Restart after power failure Contents Maintenance 41 Periodic MAINTENANCE sssaaa 25 42 Cleg ingiN pii esses 25 4 3 General COPE mossen 25 4 3 1 General recommendations for all lUGNtS mum cmnssmsmummsmsmmm 26 4 3 2 Additional recommendations for aqueous eluents 26 4 4 Replacing the piston seal mio msn 26 4 5 Running in a NEW PISTON SEO emm 31 4 6 Replacing a damaged PIStON s smsmmmmsemmssmmsmmmsmn 32 4 7 Cleaning the inlet and outlet check valves oe 4 7 1 Cleaning after removal cccccsessssssssssssssssssssssssssssssssssessesseeeesssssssssssnsnsnianeess 32 Trouble shooting Bele GOMER na AARE 35 52 FOUIS GNA ACON Sneinen 35 55 gt ENOL MESSIES i
24. ation Current concentration 0 Gradient started 1 Select main menu Set Conc Gradient press OK Set Conc Gradient 2 The display shows Set Concentration B Turn the dial to select menu Set Gradient Length press OK Set Gradient Lengt h z 3 Set the value press OK 4 Select menu Set GradientTarget Set the gradient target value in B press OK If the pump is in Run mode the gradient will start immediately Set Gradient Target 5 Atthe end of the set gradient length time the pump continues to run at the target concentration In UNICORN select instruction Gradient in System Control Manual Pump 3 8 Ending the run and storage The pump can be left filled with a buffer overnight Note If buffers or water are stored at room temperature there is a risk that bacterial growth may occur If no further runs are planned the pump should be flushed immediately with pure eluent If aqueous buffers have been in use flushing with pure distilled water is particularly important to prevent salt precipitation Overnight storage The pump can be left filled with a buffer Weekend and Long time storage Flush the pump with water and then fill it with 20 ethanol AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 25 Operation 26 3 9 Changing eluent CAUTION To prevent precipitation of crystals when changing from a salt containing buffer to organic solvent always flush through the syst
25. ation of the pump it is important hat this running in procedure is followed The pump heads are filled with 20 ethanol for protection during transportation This is removed by running in the pump with a liquid such as distilled water which is miscible with 20 ethanol After running in the pump is primed for the chromatographic run Note If for any reason the pumpheads are dry actions according to section 4 5 Running in a new piston seal must be carried out instead 2 9 2 Running in 1 Purge the pump see section 2 8 Purging the pump 2 Switch the mains power switch on the rear panel to ON 3 Check that the outlet tubing are not blocked and press OK in the pump synchronization display 4 Set the flow rate in menu Set Flow Rate Run both pumps at the same time by setting the concentration to 50 in menu SetConcentrationB under menu SetConc Gradient Set the pump in Run mode Run the pump at the flow rates and times specified below Pump Flow rate Time P 901 40 ml min P 903 4 ml min 10 minutes 10 minutes 5 Stop the flow by setting the pump in End mode AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 17 Installation 18 2 10 Connecting the pump outlet tubing WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are in use Connect the tubing by first inserting the tubing fully then tightening the connector fingert
26. block the ventilation inlets or aoutlets on the system Place the instrument directly on the bench Do not use any soft material under the instrument to ensure that the ventilation inlet in the front is not blocked 2 3 Connecting electrical signal cables The sockets for electrical signals are located on the rear panel Pressure analog qy UniNet 2 Remote UniNet 1 E oD 2 X _ SE ees L Ses Mains WARNING Pump P 900 must be connected to a grounded mains socket to prevent system parts from becoming live WARNING Do not block the rear panel of the system The mains power switch must always be easy to access pe AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 13 Installation b p 2 3 1 Connecting to chart recorder if used 1 Connect the chart recorder to the Mini DIN socket Pressure using the cable supplied Pin Signal 1 Signal 2 Signal ground 3 6 Not used Reserved for factory testing Note The signal cable is delivered with protective covers on each wire Do not remove the protective covers from unused connections as a short circuit may disturb the measurements 2 Set the recorder to 0 1 V input full scale 3 Refer to section B 2 2 for a description of setting the full scale pressure 2 4 Connecting to communication link The pumps used in AKTAexplorer and AKTApurifier are
27. can be gained from the pressure trace see section 5 4 Checking the pump pressure for more information WARNING The instrument must not be opened by the user It contains high voltage circuits which can give a lethal electric shock 5 2 Faults and actions If the suggested actions do not correct the fault call GE Healthcare Fault Action No text on the front display 1 Check that the mains cable is onnected and the power switch is in ON position I 2 Check the mains power supply Erratic flow noisy baseline signal irregular pressure trace Air bubbles passing 1 Check that there is sufficient eluent present in the through or trapped in the pump reservoirs 2 Check all connections for leaks 3 Follow the instructions in section 5 5 Removing trapped air bubbles from the pump Inlet or outlet check valves 1 Follow the instructions in section 4 7 Cleaning not functioning correctly the inlet and outlet check valves 2 Clean the valves in place If improvement is not seen remove the valves and clean again Piston seal leaking 1 Replace the piston seal according to the instructions in section 4 4 Replacing the piston seal Blockage or part blockage 1 Flush through to clear blockage 2 If necessary replace tubing 3 Check inlet tubing filter It can become clogged if unfiltered buffers or samples are applied See instructions for flushing through at the end of the run in section 3 8 Ending the run and storage
28. drain plate and the rinse chamber Remove and discard the rinsing membrane Remove the rinse chamber For P 903 remove also the support washer Drain plate Rinsing membrane Rinse chamber S 10 Gently withdraw the piston seal Discard the used seal 11 The pump head rinse chamber and drain plate should be carefully rinsed or cleaned in an ultrasonic bath if available If dirt can be seen on any surfaces the inlet and outlet check valves should be removed and cleaned separately see section 4 7 12 Wet the new seal slightly and place it in the hole on the pump head and press it down into position with a hard flat object For P 903 refit the support washer on top of the new seal 13 With the pump head still facing downwards on the bench place the rinse chamber onto the head with the rinse ports in line with the inlet and outlet check valves The conical depression in the rinse chamber should be facing upwards ready to accept the new rinsing membrane Fit the rinsing membrane with the conical face downwards 30 KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Maintenance Piston Return spring Piston Drainage hole Drainage hole Drain plate Drain plate Rinse membrane Rinse membrane Rinse chamber Rinse chamber Rinse chamber Rinse chamber outlet outlet Support washer E Inlet Inlet Piston seal ae NECK Valve Jointing ring check valve Piston seal Outlet CP lt utlet c
29. e Connect the tubing using male 5 16 connections Purging the pump CAUTION To protect the piston seals the pump must never be run with air in the inlet tubing Follow the procedure below to remove the air Purge valve Purge tubing a 7 E Fill a reservoir flask with distilled water Immerse the inlet tubings of both pump modules with filters in the water Note Never place the reservoir flask below the level of the pump inlet Connect a male Luer syringe of about 30 ml to the open end of the purge tubing Connect the male Luer connector at the other end of the purge tubing to the left purge valve at pump module module A Turn the purge valve counterclockwise half a turn to open it and slowly draw eluent to the syringe When fluid starts to enter the syringe continue to draw a few millilitres before closing the purge valve Check that there is no air left in the inlet tubing Repeat steps 3 to 5 for pump module B if fitted AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Selftest Pump synchronization Set Flow Rate Run 0 10ml min 0 00MPa 0 0 B End 0 00mI min 0 00MPa 0 0 B Installation 2 9 Running in the new pump 2 9 1 General To be able to follow this section you must know how to operate the pump Read through section 3 Operation before continuing Note To ensure trouble free oper
30. e 1 18 1128 47 nlet manifold 10 ml UNF 5 16 1 18 1120 80 Recorder cable 1 18 1110 64 Check valve kit including 1 18 1128 66 1 inlet check valve 1 outlet check valve Switch Valve SV 903 1 18 1114 49 ixer M 925 18 1118 89 ixer chamber 0 6 ml 1 18 1118 90 Mixer chamber 2 ml 1 18 1118 91 Mixer chamber 5 ml 1 18 1118 92 ixer chamber 12 ml 1 18 1118 93 Purge valve 1 18 1128 87 O ring for purge valve 10 19 0036 01 nlet filter set 10 18 1113 16 nlet tubing kit i d 1 8 o d 3 16 1 18 1114 40 nlet tubing kit i d 1 0 mm o d 1 8 1 18 1119 26 Tubing kit for rinsing system 1 18 1113 32 Purge kit 1 18 1124 53 nlet filter assembly 2 18 1113 15 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 59 ES Reference information Item Quantity per pack Code no Teflon tubing i d 1 8 o d 3 16 3m 18 1112 47 Teflon tubing i d 0 063 o d 1 8 3m 18 1121 16 Tubing connector for 1 8 o d tubing 10 18 1121 17 Tubing connector for 3 16 o d tubing 10 18 1112 49 Ferrule for 1 8 o d tubing 10 18 1121 18 Ferrule for 3 16 o d tubing 10 18 1112 48 Stop plug 5 16 5 18 1112 50 Stop plug 1 16 5 18 1112 52 Union Luer female 1 16 male 2 18 1112 51 Union 1 16 female M6 male 6 18 1112 57 Union M6 female 1 16 male 8 18 1112 58 PEEK tubing i d 0 50 mm o d 1 16 2m 18 1113 68 PEEK tubing i d 0 75 mm o d 1 16 2m 18 1112 53 Teflon tubing i d 0 75 mm o d 1 16 2m 18
31. e 1 Connection for an additional switch valve for buffer selection when used as stand alone The instrument contains no internal user replaceable items Connect any auxiliary equipment to the 9 pole D SUB female REMOTE connector 5 V TTL signals only Pin Signal Function Active status low or closed terminal to pin 5 0 V 1 Remote run pause active pause 2 4 5 OV Signal ground 6 Pressure alarm output active alarm 7 9 When used in combination with UNICORN and a custom designed strategy 4 digital inputs and 4 digital outputs can be handled via the REMOTE connector Pin 1 4 can then be used as inputs and pin 6 9 as outputs All input output signals are 5 V TTL signals AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information A A 2 Pump head The pump consists of one or two pump modules A and B Each pump module consists of two pump heads The individual heads are identical but are actuated in opposite phase to each other by individual stepper motors controlled by a microprocessor This gives a continuous low pulsation liquid delivery Each outlet check valve houses a purge valve a fingertight connector It is used for draining any unwanted eluents or to remove air from the system The purge valve is opened by turning it counterclockwise half a turn The inlet to each pump head is fed from a common manifold Solvent is drawn up into the pump head through a non return check valve by
32. em with water as the intermediate liquid When changing from one eluent to another it is extremely important that the two eluents are totally miscible with one another If the two eluents are immiscible the pump should be flushed first with an intermediate liquid which is miscible with both eluents Failure to do this will cause a wrong flow of eluent from the pump When changing from a salt containing buffer to an organic solvent use water as the intermediate liquid to prevent precipitation 1 Stop the pump by setting it in Pause mode 2 Transfer the inlet tubing into the new eluent or into the intermediate liquid 3 Run the pump at a flow rate and time as specified in the table in section 2 9 4 Stop the pump If an intermediate liquid is being used transfer the inlet tubing into the final eluent and repeat step 3 with the new eluent In UNICORN select instruction PumpWash in System Control Manual Pump 3 10 Restart after power failure If the power supply to the instrument is interrupted the instrument automatically restarts itself doing selftest and prompting for synchronisation All values under the Setup menu are retained Other values e g flow rate are reset AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Maintenance 4 Maintenance Note The piston seals have a limited life time depending on the flow rate pressure and eluents used The seals are regarded as consumables and are available a
33. ensor Range Offset error Scale error Environment AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 0 01 100 ml min in steps of 10 l min 0 01 100 ml min in steps of 10 l min 0 01 200 ml min in steps of 10 pl min 0 001 10 ml min in steps of 1 ul min 0 001 10 ml min in steps of 1 ul min 0 001 20 ml min in steps of 1 ul min 0 10 MPa 100 bar 1450 psi 0 25 MPa 250 bar 3625 psi 1 13 1 14 lt 1 day exposure Max 5 cP 2 or 20 ul min whichever is greater 2 or 2 ul min whichever is greater with compression compensation activated rsd lt 0 5 rsd lt 0 5 lt 1 at 0 5 100 ml min rsd lt 0 25 at 0 5 100 ml min lt 1 at 0 05 10 ml min rsd lt 0 25 at 0 05 10 ml min 0 27 5 MPa lt 0 05 MPa lt 2 4 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar 55 Reference information C 2 Physical data Delay volumes Total volume between inlet and outlet per pump module P 901 P 903 Inlet and outlet tubing Control Degree of protection Housing Wetted materials Piston Pump head Pump seal P 901 P 903 Check valve Output manifold Chemical resistance Power requirement Power consumption Digital input Digital output Recorder output Functions Display Dimensions H x W x D Weight lt 800 ul module lt 600 ul module UNF 10 32 2B Fingertights with capillary tubing 1 16 outer diameter Stand alone or from a PC running UNICORN
34. gination at work and GE monogram are trademarks of General Electric Company Microsoft and Windows are either trademarks or registered trademarks of Microsoft Corporation in the United States and or other countries All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2005 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamhire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Huakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Paci c Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 e Austria Tel 01 57606 1619 Fax 01 57606 1627 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 8
35. h with distilled water 4 Refit the check valves The inlet check valve with a lip for the manifold and a larger diameter opening is fitted to the side marked IN of the pump head Tighten the valves until fully finger tight and then use the 13 mm wrench to tighten a further 1 3rd 1100 of a turn Do not over tighten the valves since damage to the internal components can occur WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are in use Connect the tubing by first inserting the tubing fully then tightening the connector fingertight Finally tighten the connector a further 1 4 turn using the key supplied 5 Refit the outlet tubing and the inlet manifold 6 Purge the pump carefully and check that the pumping action has been corrected See section 2 8 Note Check valves have precision matched components and should only be disassembled further by a trained service engineer If the problem cannot be corrected the valve should be replaced completely AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 35 Maintenance 36 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Trouble shooting 5 Trouble shooting 5 1 General When contacting GE Healthcare for support state the program version of the instrument shown for 2 seconds during switch on It is recommended to make a pressure recording since much information
36. heck valve check valve P 901 P 903 14 Place the drain plate on top of the assembly Use the two screws to lock the complete assembly together Note Align the drainage hole in the drainage plate with the inlet check valve the opposite side of the pump head marked OUT UP Inlet check 15 Wipe clean the piston and remove all finger prints Wet the piston and then insert it into the return spring With the pump head facing downwards on the bench insert the piston into the pump head by pushing it gently but firmly vertically downwards into the seal CAUTION Do not push the piston at an angle to the head and DO NOT twist the piston AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 31 Maintenance 16 Turn the head so that the inlet valve and drainage hole are facing downwards and the text UP OUT on the pump head is facing upwards Mount the complete pump head over the locating pins on the front panel Locate the metal end of the piston and the spring towards the drive cam Hold the pump head firmly against the side panel of the housing with one hand Do not allow the assembly to twist under pressure from the return spring Using the Allen key fit and tighten one of the Allen screws Fit and tighten the remaining Allen screw WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are in use Connect the tubing by first
37. ight Finally tighten the connector a further 1 4 turn using the key supplied Remove the blind plug from the pump head outlet manifold Connect the outlet tubing using 1 16 Fingertight connectors Place the end of the tubing in a drain or waste collection vessel Close the purge valve by turning it clockwise Purge valve Outlet tubings Set the flow rate to 10 ml min for P 901 and to 1 ml min for P 903 Set the pump in Run mode Allow the pump to run for 1 2 minutes to prime the new outlet tubing Stop the flow by setting the pump in End mode 2 11 Installation of accessories Mixer M 925 and up to three 2 way Switching Valves SV 903 can be connected to the pump Mixer M 925 Switch Valve SV 903 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Installation CAUTION Before connecting any accessories make sure the power switch is in OFF position Mixer M 925 is connected to the UniNet 2 connector SV 903 valve for buffer selection is connected to Valve 1 The valve is set on off via the pump sub menu Set Valve 1 under the Setup menu See Reference information B 2 8 SV 903 valves for BufferPrep are connected to Valve A and Valve B SV 903 for one pump gradients is connected to Valve A Pressure anolog qy UniNet 2 Remote UniNet 1 m wo E F Out to system O Fluid inlet tubing KTAdesign Pump P 900 User Manuall 18 1120 04 Edition
38. in the upper left corner of the display Available actions are shown at the Current Mode SAR lower right There are four different displays for this menu and the menu ee displayed will depend on the current mode When a new mode is selected mode the appearance of the menu will change End 1 00ml min Pressing OK in a mode changing display will select the underlined mode Different modes can be underlined by turning the dial Pause 1 00ml min To start the pump Select Run and press OK To stop the pump Select End and press OK Hold 1 00ml min 3 4 3 Preparation before starting CAUTION Before the start of each run always ensure that there is an adequate supply of eluent in the reservoirs Never allow the pump to run dry since this will affect the lifetime of the piston seals 1 Check that there is sufficient eluent present for the run and that the solvent filter is fully immersed If the eluent is to be changed see section 3 9 Changing eluent a Note The pump may not work if the buffer vessels are sealed Do not close the vessels off completely AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 23 Operation Set Flow Rate Set Conc Gradient Set Concentration B 24 2 If there is air in the tubing or the pump prime the pump Refer to section 2 8 Purging the pump 3 Check that there is sufficient liquid 20 ethanol in the rinsing system reser
39. in valve x 1 Switch off the instrument Check connections 2 Check the connections to the valvels 3 Switch on the instrument ERROR Pump too warm 1 Switch off the instrument to let it cool Check fans 2 Clean or clear the front air inlet 3 Switch on the instrument and check that the fans are running ERROR Sunc failure 1 Switch off the instrument Please restart 2 Switch on the instrument Remember to open the purge valves before synchronization ERROR key OK 1 Switch off the instrument ERROR key Esc 2 Check all connections ERROR key OK Esc 3 Switch on the instrument ERROR 100 ERROR 109 113 ERROR 120 121 ERROR 106 108 1 Switch off the instrument ERROR 118 2 Check all UniNet1 and UniNet 2 connections 3 Switch on the instrument 38 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Trouble shooting ee 5 4 Checking the pump pressure To check the pump function a recording of the pressure can be made see section B 2 2 or by checking the pressure in UNICORN This pressure recording is more sensitive than the reading on the display By observing the piston stroke indicator in the Check menu see section B 1 1 together with the pressure trace the pump head which is functioning abnormally can be identified There can be several causes of an abnormal pressure recording for example e air trapped in the pump heads e partially blocked solvent filters e leaking connections e piston seal leakage e check valve ma
40. inserting the tubing fully then tightening the connector fingertight Finally tighten the connector a further 1 4 turn using the key supplied 17 Reconnect the outlet tubing to the outlet check valve and the manifold block as described above 18 Reconnect the inlet manifold and if used the switch valves 19 Refit the tubing of the piston seal rinse system 20 The pump should now be purged and the new piston seal carefully run in following the instructions in section 4 5 32 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Maintainence 4 5 Running in a new piston seal The piston seal should be run in using 100 methanol CAUTION To protect the pump seals always ensure that there is a constant supply of eluent The pump should never be allowed to run dry 1 Ensure that the reservoir is filled with sufficient eluent Immerse the inlet tubing in the eluent The reservoir should be placed at least 30 cm above the pump inlet 2 Connect a male Luer syringe of about 30 ml to the open end of the purge tubing 3 Connect the male Luer connector at the other end of the purge tubing to the left purge valve at pump module module A 4 Turn the purge valve counterclockwise half a turn to open it and slowly draw eluent to the syringe 5 When fluid starts to enter the syringe continue to draw a few millilitres before closing the purge valve Check that there is no air left in the inlet tubing
41. ion function can be selecteded to compensate for flow losses due to compression at high pressure When the compensation function is selected the pump takes into consideration the stated compressability for the liquid at the actual pressure For methanol at 25 MPa for example the loss in flow is reduced from 10 to 1 However since this compensation may affect the reproducability it should only be used when exact flow rate is required A compressability value between 4 0 x 10 Pat 15 0 x 10729 Pat can be stated Some common values are given below Liquid Value in Pa Water 4 6 x 107 Methanol 12 1x 107 Most organic solvents 415 11x 1072 Note The compensation function is only available for P 903 using 10 ml pump heads AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 49 B Reference information 1 Select main menu Setup press OK SE GA CE 2 Select sub menu Setup Compr comp press OK off off on 3 Select On to activate or Off to deactivate compensation Press OK Setup Comp A 4 0E 10 4 0 4 Enter a compensation value between 4 0 and 15 0 for pump module A Setup Comp B i a0 16 n 5 Enter a compensation value between 4 0 and 15 0 for pump module B B 2 8 Set mixer operation Sets the condition of the mixer connected to the pump 1 Select sub menu Setup Mixer press OK Setup Mixer 2 Select either auto off or on press OK Auto means the mixer is
42. lect either MPa bar or psi press OK B 2 4 Set gradient base Sets the base for the gradient to either time or volume 1 Select sub menu Setup Gradient Base press OK 2 Select either time or volume press OK AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information CE B 2 5 Set gradient mode Sets one pump gradient 1 two pump gradient 2 or double D flow rate mode The one pump mode is used for low pressure gradient formation with switch valve SV 903 Two pump mode is only available with both A and B pumps installed and varies the speed of each pump for high pressure gradient formation Double mode allows for double max flow and is only possible with both A and B pump modules installed In double mode the two pump modules are working in parallel with Conc B locked to 50 1 Select sub menu Setup Gradient Mode press OK Setup Gradient Mode 2 Select either 1 2 or D press OK Note Selecting one pump gradient mode disables the B pump module Gradient mixing is not possible in double mode B 2 6 Set pump type Sets the size of pump modules used for pump A and B either 100 ml min or 10 ml min 1 Select sub menu Setup Pump A Type press OK Setup Pump A Type 2 Select either 100 ml or 10 ml press OK Setup Pump B Type 3 Select sub menu Setup Pump B Type press OK 4 Select either 100 ml or 10 ml press OK B 2 7 Set compression compensation A compensat
43. lfunction e piston damaged Some examples of normal and abnormal pressure traces together with comments are shown in the following table Result Running conditions Diagnosis P 901 Flow rate 1 00 ml min Pump performance Left Right Left Pressure 2 0 M Pasatisfactory Recorder 1 V 1 mm s Active head left and right are shown os Mea Pump setup Full Scale The small pressure peaks are caused by the EE 10 MPa pump principle Flow rate 30 ml min Pump performance satisfactory oe ef aga a AA Pressure 7 5 MPa os Mpa Recorder 1 V 1 mm s is Pump setup Full Scale 10 MPa P 903 Flow rate 1 00 ml min Pump performance satisfactory erecta Pressure 12 0 MPa Recorder 1 V 2 mm min 1MPa Pump setup Full Scale 5 min 25 MPa AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 39 Trouble shooting LL Result Running conditions Diagnosis Flow rate 1 00 ml min Left pump head OK right Pressure 2 0 MPa pump head not working Recorder 1 V 1 mm s Possible causes Pump setup Full Scale e Bad or missing seal 10 MPa e Large leakage in inlet check valve e Inlet opened or blocked Flow rate 20 ml min Air bubbles passing through pump heads ORON NNE raaa Brass ure 4 3 MPa then the trace returns to normal os Ae Recorder 1 V 1 mm s me Pump setup Full Scale 10 MPa P 903 Flow rate 2 ml min One pump head stops Pressure 4 3 MPa working Possible causes Recorder 1 V 1 mm s e Air bubble stuck in pump Pump setup Full Scale head os
44. loosen the tubing connector on the outlet valve 3 If the pump is equipped with Switch Valves SV 903 first remove the switch valves and disconnect the tubing Unscrew the two white knurled screws under the pump to release the support bracket locking the inlet manifold into the inlet valve Gently lower and remove the complete manifold 4 Using the Allen key unscrew and completely remove one of the two Allen screws locking the pump head in position 5 When unscrewing the second locking screw push firmly on the front face of the pump head to compensate for the pressure of the piston return spring Hold the pump head firmly to prevent it from twisting Remove the second screw and without allowing the pump head to twist sideways carefully pull it out 6 Place the pump head face down on the bench Pull out the piston together with the return spring AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 29 Maintenance 7 Inspect the piston and return spring for sign of damage If damaged they should be replaced 8 Wipe the piston with a clean cloth If salt solutions have been used the piston may be slightly corroded This corrosion can be removed with a rubber eraser If it cannot be wiped or rubbed clean scrape off any deposits with a scalpel or razor blade Inspect the piston with a magnifying glass for scratches Replace with a new piston if any scratches or cracks are found 9 Remove the two screws securing the
45. mina n R RRi 36 5 4 Checking the PUMP PreSSUl s smmmsmmmsemmmmsmsmssmsn 37 5 5 Removing trapped air bubbles from the PUMP 38 Description AL INSTUN GIG ceevsyssscesichesstsecticetuliveenatiadaiinddinnanaedansin 39 A2 Pump OCG aissasss cess cscessosssescecetbcevsnsssveqaassdsearvaseel dccoaasserseessccecsusteees 41 A3 PUPP Principle erac 43 A4 Outlet MOI 0 6 cc ec ce 43 A5 Pressure MONItOTING sssini 44 A 6 Using an external chart recorder smssmmmsemmmsm 44 KTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Contents ee B Menus B 1 Check Menu B 1 1 Checking piston stroke B 1 2 Check pump operation tilMe momsen 45 B 1 3 Check number of piston strokes mums 45 B 1 4 Check Service Mode ueessssssssssssessssssssssssssessseseeceeceeeeesesssessstitutmnttnnnnnnsnnssssete 45 B 1 5 Calibrate Pressure Offset mu 46 B2 SOLUP IMIG NU sanien n EERS 46 B 2 1 Set pressure liMit cesses 46 B 2 2 Set pressure full scale ON recorder 46 B23 Set pressure UNM cscs cstrsscczesccatbspstetdescesasiotbessssounsasilonsBscageaasbeassssagainncibeee 46 B24 Setgradient OSC essensen snara 46 B 2 5 SOL QFAGICE FIO Ss saassscesessazosacincssossbisonssssnsaniezsszasetslescesrancjaestebionisncevtaisains 47 Bi Zs SOL PUMPY Eniris iaaio ENEE NA EES 47 B 2 7 Set COMPFESSION COMPENSATION eesssssssssssssssesssssessssseesseeeeesssaeeeseeeseeee 47 B 2 8 Set mixer OPEN OtiON amssmsussurasrssneranersnersner anar
46. mooth low pulsation flow from the pump outlet manifold Pump P 900 features e P 901 Pressure range 0 10 MPa 100 bar 1450 psi Flow rate range 0 01 100 ml min isocratic and gradient mode Double mode flow rate range of 0 01 200 ml min isocratic mode e P 903 Pressure range 0 25 MPa 250 bar 3625 psi Flow rate range 0 001 10 ml min isocratic and gradient mode Double mode flow rate range of 0 001 20 ml min isocratic mode e The pumps are equipped with 2 pump modules A and B including two pump heads each This allows for binary gradients with high pressure mixing e All pumps has a pressure sensor connected to pump module A e Low pulsation e Switch valve control for low pressure gradients and BufferPrep AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 41 Reference information 42 Valve 1 Valve A Valve B analog out 0 1y UniNet2 Remote UniNet 1 W mE D Mains EJ Connector switch Function Pressure analogue out 0 1V Pressure signal to chart recorder UniNet 2 Connection to mixer M 925 and UniNet 2 network Remote Input for remote on off and output for pressure alarm UniNet 1 Connection UniNet 1 network Mains Supply voltage grounded 0 1 Instrument on off switch Valve A B Connection to switch valves A and B used for BufferPrep When pump used for one pump gradients as stand alone valve A should be used Valv
47. n and piston seal on the non pressure side of the piston seal This protects the piston seals and pump heads by preventing a build up of deposits from the solvents 1 Connect the tubing for the piston seal rinsing system The connections are simple plug in fittings 2 The three lengths of wide bore tubing connect the pump heads in series connecting the top of one pump head to the underside of the next The wide bore tubing with the check valve should be fitted between the right pump head of pump module A and the left pump head of pump module B The arrow on the check valve must point towards pump A 3 Two lengths of narrow bore tubing are also supplied with the pump Connect the tubing with a Union Luer connector to the underside of the left pump head on pump A Connect the other to the top of the right pump head of pump module B and immerse the tubing in a flask containing rinsing solution 20 ethanol P 901 P 903 4 Connect a syringe to the rinsing tubing that is connected to the underside of the left pump head on pump A Slowly suck up rinsing solution to the syringe When rinsing solution starts to enter the syringe continue to draw a few millilitres 5 Loosen the syringe and immerse the tubing in the rinsing solution AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 15 Installation e 2 8 Connecting the inlet tubing The inlet tubing is connected to the centre port of the inlet manifold of each pump modul
48. nt values I Check Check internal operating values See Reference information section B 1 I Setu p Setup language pressure limits etc See Reference information section B 2 I Alarm Timer 12 30 52 Set different timer options The pump can be started or stopped at set times See Reference information section B 3 22 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Operation 3 4 Starting and stopping the pump WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are in use Connect the tubing by first inserting the tubing fully then tightening the connector fingertight Finally tighten the connector a further 1 4 turn using the key supplied 3 4 1 Main operating menu ESSER The main operating menu shows the current flow rate together with a mode 2 00MPa 45 5 B indication pressure and B if used The available modes are Run The pump is running with set flow rate End The pump is not running Flow rate and gradient are reset Pause The pump is stopped but the set flow rate value and the gradient are retained Hold The gradient is held at the value displayed and the pump continues to run 3 4 2 Mode changing menu z Mode changes are made in the mode changing menu above the main Run 1 00ml min 3 i F F operating menu turn dial counter clockwise The current mode is shown
49. ontact an authorized representative of the manufacturer for information concerning the decommissioning of equipment AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Contents Contents Introduction LL GQ RICK Cl ee a USSR TEN RT RASAETIET TIRANA 7 PLD AGCOSSOMCS ctsic 202ssesestsss siactoratescadeshcttecctstletistetsuselen ta i iiai 8 E SOU EEE EATE ETTA 9 Installation 2 UNPACKING sieisen 11 2 2 General preCOUtiONS smssmssmmmmsmsmmsemmnsmsmmsssnsn 11 2 3 Connecting electrical signal cables e emsmsmmmmssm 11 2 3 1 Connecting to chart recorder if used 2 4 Connecting to communication link 2 5 CONNECTING tO supply voltage s mmmsmssmsmssmsmsmmm 2 6 Installing the piston seal rinsing SYSteMu e ee 13 2 7 Connecting the inlet tubing s smssmsmssmsmsmmm 14 2 8 Purging the PUMP ess 2 9 Running in the new pump LIA GOCE legs bredder tr bords nrg kn RES AS SNR 292 FREGE iranu EEEE OEE ONTE 2 10 Connecting the pump outlet tubing e ee mmm 16 2 11 Installation of accessories Operation S COM OW ean n aE E 19 3 2 Menu selection and settings s s 1s 11911 1911151115111511 10111511 1011100 19 3 2 1 Menu SELES OM senine inausi 19 3 2 2 Return CO MAIN MENU cesssssssssssssssessesssssssesseseececeeeeenensnssnsnsnnennsitsesseseseeeeeees 20 32 3 SOIC Value isneus ninn inenen 20 3 3 MAIN MENU overview uses 20 3 4 Starting and stopping the
50. r 10 10 XT Equipped with four pump heads in two pump modules Out to system Fluid inlet tubing P 901 P 903 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 9 Introduction 10 All P 900 models are high performance laboratory pumps for use in liquid chromatography and other applications where accurately controlled liquid flow is required Double mode can be selected to double the flow rate range Pump P 900 features e P 901 Pressure range 0 10 MPa 100 bar 1450 psi Flow rate range 0 01 100 ml min isocratic and gradient mode Double mode flow rate range of 0 01 200 ml min isocratic mode e P 903 Pressure range 0 25 MPa 250 bar 3625 psi Flow rate range 0 001 10 ml min isocratic and gradient mode Double mode flow rate range of 0 001 20 ml min isocratic mode e The pumps are equipped with 2 pump modules A and B including two pump heads each This allows for binary gradients with high pressure mixing e All pumps have a pressure sensor connected to pump module A e Low pulsation e Switch valve control for low pressure gradients and BufferPrep All versions are run with the same software Normal or double mode as well as the size of the pump heads are stated in a Setup meny see Reference information B 2 1 1 1 Accessories Accessories can be connected to the pumps and can be controlled from the pumps or from a PC running UNICORN version
51. s a service kit The wear of the piston seals is not covered by the warranty of the instrument A typical lifetime is around 1000 run hours WARNING Remove liquid or dirt from the system surface using a cloth and if necessary a mild cleaning agent WARNING Always disconnect the power supply before attempting to replace any item on the instrument during maintenance A AN CAUTION Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the instrument 4 1 Periodic maintenance Interval Action see procedures below Daily General care When required Removing trapped air bubbles from the pump Fixing leaking connections Replacing the piston seal Replacing a damaged piston Cleaning or replacing the inlet and outlet check valves 4 2 Cleaning in place Pump a cleaning or sanitizing agent through the pump The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer or distilled water WARNING NaOH is injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation nd maintenance of the system WARNING When using hazardous chemicals make sure that the entire system has been flushed thoroughly with bacteriostatic solution e g
52. ssure chamber behind the pump head can be flushed continuously with a low flow of distilled water The flexible rinsing membrane sweeps the piston and seals the rinsing system The pump head is manufactured in titanium alloy AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information A 3 Pump principle Each piston is driven by a simple robust cam eccentric These cams are driven by stepper motors via timing belts The motor speed is varied to achieve linear movement and compensation for compressibility This produces the particular motor sound This system guarantees an accurate low pulsation flow over the entire flow rate range independent of the back pressure When an increase in flow rate is programmed the motor speed accelerates gradually giving a soft start and building up speed to the flow rate required When a decrease in flow rate is programmed the motor speed reduces rapidly to the lower flow rate User Interface I Slave Main Slave CPU CPU CPU Stepper Motor Timing Belt Eccentric Piston Pump Head Ee Pressure Transducer Pump A Pump B 2 Inlet Outlet Inlet A 4 Outlet manifold The outlet of each pump is connected by tubing to a manifold block where the liquids from both heads are combined to give continuous eluent delivery The tubing and the manifold block are made in PEEK The manifold block houses
53. t buzzer is performed press OK 1 Not always pre programmed Depends on customer specific contract AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 47 B Reference information Calib Press Offset Setup Hi Press Limit Setup Lo Press Limit Setup Pr Full Scale Setup Pressure Unit Setup Gradient Base 48 8 1 5 Calibrate Pressure Offset 1 Ensure that the pump is at zero pressure 2 Select main menu Check press OK 3 Select sub menu Calib Press Offset press OK Working please wait is displayed until the calibration is completed B 2 Setup menu B 2 1 Set pressure limit Sets high and low pressure limits When the limit is reached the pump is set in Pause mode a buzzer sounds and an error message Overpressure on ascending pressure or Underpressure on descending pressure is shown The message has to be confirmed by pressing OK 1 Select sub menu Setup Hi Press Limit press OK 2 Set the value press OK 3 Repeat step 1 and 2 for Setup Lo Press Limit B 2 2 Set pressure full scale on recorder Sets the full scale pressure for the chart recorder output The full scale value will correspond to 1 V output on the analog output 1 Select sub menu Setup Pr Full Scale press OK 2 Set the value press OK B 2 3 Set pressure unit Sets the pressure unit used in the display 1 Select sub menu Setup Pressure Unit press OK 2 Se
54. the outlet connections to the mixer Outlet tubing Purge tubing Purge valve AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE 45 Reference information 46 A 5 Pressure monitoring The pressure in the system is continuously monitored by the pressure transducer housed in the outlet manifold block of pump module A In a two pump module configuration with the pump modules connected to a mixer the pressure is almost the same in pump A and B The output pressure is shown in the display together with the flow rate A continuous recording of the pressure may be made using the analog output For the protection of the system a maximum and minimum pressure limit can be set A 6 Using an external chart recorder The facility for recording the actual pressure at the pump outlet can be used for recording the pressure during an overnight run and for checking the pump function for any abnormality The external chart recorder output for pressure is 0 1 V The full scale range is set under sub menu Set Pr Full Scale under Setup see Reference information B 2 2 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE B Menus Reference information CE Check Heads 2 30MPa Check Total Run Time Check Piston Strokes Check Service Mode Telephone Service 012345678901 Contract Number 012345678901 Serial Number 01234567 YM 012345 Pump P 900
55. version 2 20 or higher through UniNet 1 cable connection IP 20 Aluminium oxide Titanium alloy PE polyethylene and stainless steel Elgiloy PTFE polytetrafluoroethylene and stainless steel Hastelloy PTFE polytetrafluoroethylene PVDF polyvinylidene luoride titanium and ruby sapphire PEEK Kalrez and stainless steel Hastelloy The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 Ethylacetate 100 Hexane and 100 Tetrahydrofuran THF 100 240 V AC 50 60 Hz Up to 400 VA including accessories 5 V TTL low or contact closure see section A 1 for pin significance TTL open collectors 0 1 V full scale Languages selectable English German Spanish French Italian 2 rows with 20 characters each 150 x 260 x 370 mm 17 kg 56 AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Reference information Compliance with standards The declaration of conformity is valid for the instrument only if it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except for alterations described in the User Manual e connected to other CE labelled GE Healthcare modules or other products as recommended Safety standards This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C2
56. voir 4 Set the pressure limit Refer to section B 2 1 in Reference information If the pressure limit is exceeded the pump is stopped 3 4 4 Emergency stop By pressing ESC repeatedly and ending with OK the pump is set to Pause mode 3 5 Setting the flow rate and starting the pump 1 Select main menu Set Flow Rate press OK 2 Set the value press OK 3 Select the mode menu select Run press OK The pump speed gradually increases to deliver the set flow rate 4 To change the flow rate while the pump is running perform steps 1 and 2 again 5 To stop the pump select the mode menu select End press OK In UNICORN select instruction Flow in System Control Manual Pump Click on Execute and the pump will start 3 6 Setting concentration B A percentage of eluent B can be set 1 Select main menu Set Conc Gradient press OK 2 The display shows Set Concentration B press OK 3 Set the B value press OK In UNICORN select instruction Gradient in System Control Manual Pump Set length to zero AKTAdesign Pump P 900 User Manuall 18 1120 04 Edition AE Operation 3 7 Running a simple gradient Gradients can be run in time or volume base The default is time base To change base see B 2 4 in section Reference information The gradient is run from current concentration to target concentration in set length of time or volume The gradient can be set in any operating mode 100 Gradient length gt Target concentr
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