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NeoSuck RT

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1. LVDT Imm My Recent Documents 1 LOAD CELL Places SUCK PRESSURE c Time s 1 Save in D test EMG OOS F uv ex E3 Testing D61206 dat My Network File name Baby A 01 0204 Y Save as type Data files dat ad NeoSuck RT V Beep On Off Real Time Observations Cancel 1 0 4 0 5 0 6 Amplitude cr H20 0 5 Pause s Period s Blocks Sampled 0 Figure 6 Recording new data 9 Dale Created 6 15 2006 3 49 52 PM Then user can press the Start to begin the recording or Pause button to halt it NeoSuck RT CNL U of Kansas 2 2 Analyze existing data by NeoSuck RT Based on the data collected described in section 2 1 Record new data by NeoSuck user can systematically apply analysis tools in NeoSuck RT to investigate more detailed properties of the human infant NNS 2 2 1 Basic analysis Click File Open an open file dialogue will pop up Figure 7 to enable the user to designate the name and path of the target data file za NeoSuck RT C Program Files Neuro Logic NeoSuck RTWata test Baby_A_010206 dat MOTOR PULSE v EMG OOS F uv Look in gt test e ey E3 1 i Baby A n10206 dat i5 l TestingA_061206 dat My Recent Documents Desktop SUAE My Documents 33 My Computer My Network File name Testing4_061206 dat Y f z Places SUCK PRESSUR
2. 1033 N Max 5o 0 cm H20 V Auto Scale Iv Auto Scale MV Auto Scale Iv Auto Scale EMG OOS R EMG 005 L EMG ODI R EMG DOI L Y Axis Y Axis Y Axis Y Axis Min 400 0 uv Min 500 0 uv Min 500 0 uv Min 500 0 uv Max 400 0 uv Max 500 0 uv Max 500 0 uv Max spoon uv Iv Auto Scale Iv Auto Scale Iv Auto Scale Iv Auto Scale tea Settings Histogram Amplitude Histogram m Pause Histogram Burst Period Histogram Lower Bound bo cm H20 Lower Bound s Lower Bound bo s Upper Bound 50 cm H20 Upper Bound 0 0 s Upper Bound no s Intervals i Intervals Intervals 0 Y Axis Y Axis Y Axis Min 0 Min 0 Min 0 Max 40 Max 30 Max 30 v Auto Scale v Auto Scale iv Auto Scale VW coe Figure 16 Graph and Histogram setting dialogue The Suck Pr Analysis setting Figure 17 defines parameters for peak selection and suck pressure threshold value for recording etc Settings Suck Pressure Analysis Derivative Peak Settings Threshold 0 3 Peak Valid Range 200 Peak Period 400 Suck Pressure Threshold 2 0 cm H20 Lowpass Cutoff Frequency 8 Hz Skip Samples Display m oms Figure 17 Suck Pr Analysis setting 17 NeoSuck RT CNL U of Kansas 3 References Estep M Barlow S M Stumm S Fees M Finan D Seibel L Poore M Cannon S 2005 Non nutritive Suck Burst Parametrics in Preterm Infants Society for Pediatric Res
3. these setup parameters should not be modified unless the specific intent is to change file structure and sampling rates DAG Device Al Channels Motor Pulse LYDT Load Cell Suck Pressure Al Channel AcHd e Channel adi l Al Channel lach l Al Channel AacH3 l Physical Unit 1 v Physical Unit 5 mm Physical Unit 05 N Physical Unit 10 cmH20 Voltage V Voltage 0 56 V Voltage 0 484 y Voltage jg M A D Min 5 V A D Min ires es A D Min tC A D Min i0 v A D Max 5 v A D Max n v A D Max i Ey A D Max o v EMG 005 R EMG OOS L EMG DDI R EMG OOI L l Channel ACH4 Al Channel ACHS5 Al Channel ACHE v Al Channel ACH v Physical Unit ho uv Physical Unit hoo o uv Physical Unit ho uv Physical Unit ho uv Voltage Ze V Voltage jac i V Voltage D V Voltage Zz c M A D Min i0 v A D Min 0 v A D Min i0 v A D Min i0 y A D Max woo DEM A D Max o v A D Max IL A D Max inm Block Sample Rate 3000 Sample Time 30 No of Samples 90000 Down Sample 100 Cancel Figure 15 DAQ board settings The Graph and Histogram settings Figure 16 include the dialog windows for lower and upper limit selection according to user s preference 16 NeoSuck RT CNL U of Kansas Settings Graph Graph Settings Motor Pulse LYDT Load Cell Suck Pressure Y Axis Y Axis Y Axis Y Axis Min Y Min 8 929 mm Min 4 033 N Min 1 0 cmH20 Max 40 V Max 8329 mm Max
4. analyze the suck pressure and signals from other channels for premature and term baby The analysis tools in NeoSuck RT include histogram analysis FFT analysis bad block screening digital signal processing generate output ASCII files for time and frequency domain etc 1 1 Hardware The Hardware for this testing equipment includes gt A CPU with 1 GB RAM running Windows XP gt NI DAQ 6052E and BNC 2110 or BNC 2090 gt Bridge Amplifier gt AC PreAmplifiers 4 gt Sensors pressure sensor and EMG Ag AgCl sensors The hardware listed above is configured up according to Figure 1 and baby s suck pressure and EMG signal sampled from the Soothie silicon nipple receiver is input to NeoSuck RT for real time digitization signal processing and waveform display NeoSuck RT CNL U of Kansas Suck NN Ai3 Bridge Amplifier K Suck Pressure Ai8 E Computer and DAQ LPF 50Hz ransducer EMG EMG ics wie Ai4 7 Ai4 7 EMG Ai4 7 PreAmpliiers Sensor GND is connected to baby leg Dec 1 2005 by Joan Figure 1 Hardware configuration for NeoSuck RT 1 2 Software This software is coded by Visual Basic Net 17 265 coding lines according to the structure shown in Figure 2 The function of this software includes real time data acquisition histogram processing FFT analysis ASCII file generation and export desired data to txt file NeoSuck RT CNL U of Kansas Structure Plot
5. of NeoSuck New frmMain vb mnuFileNew Name a new dat file Open frmMain vb mnuFileOpen Open an existing dat file File Page Setup frmMain vb mnuFilePageSetup l frmPrintSelection vb Print frmMain vb mnuPrintAmplitudeHist etc ummy 1 Exit frmMain vb mnuFilePageSetup DAQ mSettinosDAQ vo 8 AI calibration settings for DAQ board Graph rmSettingsGraph vb Y Axis scale setting for 8 AI plots Settings Games imer E Histgram l poene scale setting for Histogram of Amplitude Pause and Burst Period Suck Pr Analysis fm SattingsPeakvb Peak selection settings for suck pressure plot NeoSuck frmMain vb drm Vain vo Select Baseline Amplitude rm melitudetist ub Histogram plot of cumulative amplitude Histogram Pause Period rmBurstPauseHist vb Histogram plot of cumulative bust a Burst Period l frmDerivative vo Histogram plot of cumulative pause Tools dpa mee Bad Block List AA EO A ee Bad block list selection dialog Nes dp dumis mma _ s HPF Filter frmSuckFFTSettingsFilter vb ied a Settings ay e o TN frmSuckFFTSettingsGraph vb Ene SuckFFT MMe aaa Graphlfrms J settings Tools 8 9 Generate Composite Create ASCII Blocks frmMain vb mnuToolsCreateASCII Create data file in txt for every block frmMain vb mnuToolsGenHistFiles Create data file in txt for histogram of Generate Histogram Files amplitude bust and pause Generat
6. Automatic analysis of non nutritive suck in time and frequency domain among preterm infants NeoSuck RT R Vantipalli J Wang amp S M Barlow CNL Technical Research Report 2006 5 1 18 University of Kansas Lawrence Kansas USA Reprint available at www ku edu cnl NeoSuck RT CNL U of Kansas Table of contents UNeoSUck RJ SelUpasadisdnsedersesesdienduma debbutiddeundumaq up susirs demde duo putis adiuncta EEE 3 1 1 IVI VY A iate dude ce ME 3 1 2 SOTVA atessibanidan Men eunte ti Melee eda uae Meetup doit buds 4 1 3 PROSE atin ms taa Oe coetui t atc aetcnassieveanteucteud at aaca dane boues LP ddetos 5 R E durum T m UU T m 8 2 1 Record new data by INCOSUCK RT oic o or toi HERR RU pina ubi iode butt 8 2 2 Analyze existing data by NEOSUCK RT eee rte ete ea 10 2 2 1 Basic ANALY SIS eirese a a ai 10 2 2 2 Fourier transform of SUCK pressure 52 eee aieo rte eiie tesi eoe 12 223 Other analysis Tool Sac 15 2 93 Bises niis decr T TREE MER 16 S MEM lev EET ET 18 NeoSuck RT CNL U of Kansas 1 NeoSuck HT Setup NeoSuck RT real time 1s used to record and analyze data associated with the non nutritive suck NNS including signals related to Suck Pressure EMG OOS R EMG OOS L EMG OOI R EMG OOI L which are recorded from 5 channels of NI DAQ 6052E PCI multifunction board installed in the acquisition PC with 3 kHz sampling rate on 16 bits vertical resolution We currently apply this software and hardware system to test and re
7. E fc Files of type Data files dat pro 0 4 Time s Observations NeoSuck RT Beep On Off V Real Time Blocks Sampled 0 amp Date Created 6 15 2006 3 50 58 PM Figure 7 Opening an existing data file Figure 8 shows the initial NeoSuck AT data analysis window The three histogram windows on the right display the histogram plots for NNS Amplitude Pause Periods and NNS Burst Periods for each block that the user has selected If the user 1s only interested in the analysis 10 NeoSuck RT CNL U of Kansas of data from 8 channels displayed in the main window then these three histogram windows can be closed by the user EA NeoSuck RT C Program Files Neuro Logic NeoSuck RTWata K6_033004 K6_033004 dat MOTOR PULSE V EMG 00S R uv 200 0 0 0 ACHO ACH4 200 04 LYDT mm Cumulative Amplitude Histogram EJ ACHS m a i bservations LOAD CELL N 62 5 75 0 le cm H20 50 0 87 5 100 0 IIZ 5 125 0 Amplitud ACH2 SUCK PRESSURE cm H20 20 0 eo 5 a 10 04 E l I I 0 0 10 0 20 0 30 Time s Block NeoSuck RT Block No N Real Time First Block 3 6 Peak Count 23 E Date Created 3 30 2004 1 53 32 PM Figure 8 Open NeoSuck RT window for data analysis The User can also call these windows by clicking desired window in Tools Histogram as shown in Figure 9 Furthermore the displayed histograms ca
8. ESSURE ACH3 Amplitude cm H20 I 0 0 5 0 10 0 15 0 z0 0 Frequency Hz NeoSuck RT 2 Real Time v Composite for blocks 3 5 Composite FFT Suck PrFile K6_033004_BC dft Block 5 6 Peak Count 16 Er Date Created 3 30 2004 1 53 32 PM Figure 10 Suck pressure FFT analysis In the setting menu user could set the filter parameters cutoff frequencies for Low Pass filter and High Pass filter to plot the desired frequency property Figure 11 In addition the graph setting Figure 12 enables the user to define the minimum and maximum values for both x axis and y axis for each of the three graphs shown in this window 13 NeoSuck RT CNL U of Kansas FT Suck Pressure Block 5 FFT SUCK PRESSURE cm H20 Figure 11 Filter setting in FFT analysis SUCK PRESSURE cm H20 ma a LT wae Settings Graph Figure 12 Graph setting in FFT analysis 14 NeoSuck RT CNL U of Kansas 2 2 3 Other analysis tools There are some other analysis tools embedded in NeoSuck RT to either display graphs or export txt files which could be used for extended analyses using other commercial software like Minitab M LabVIEW MATLAB MS Excel etc 1 Tools gt Bad Block List Figure 13 enables the user to delete bad blocks from the entire data set and thus generate clean analysis data for both exported data and Fourier transform analysis Bad Block List Add Remove B
9. ad Blocks Block List Bad Block List Adi Adi Add All gt gt gt Remove Remove RemoveAll RemoveAll All tea Moe corno Figure 13 Bad Block List 2 Tools Create ASCII Blocks Figure 14 performs the function of exporting recorded dat file to txt file block by block The dat is a binary file which can be read by Visual Basic The purpose of transforming data into txt file is to make the recorded data readable by y party commercial graphics and statistical software Open Look in C3 K amp 033004 ce EB a PEZETA T nid 5 ke 033004 EFI pro My Recent Documents 2 Desktop My Documents My Computer cm F ae etwork File name K6_033004 dat m aces Files of type Data files dat pra Y Cancel Figure 14 Create ASCII blocks for dat file 3 The Generate Histogram Files and Generate Amp Period ASCH Files will generate txt files for future analysis 15 NeoSuck RT CNL U of Kansas 2 3 Other setups Some other setups in Setting of the NeoSuck RT menu bar enable the user to set and modify analysis criteria for data acquisition graphs histogram plots and FFT analysis Figure 15 is the DAQ setting dialog where the user can adjust the minimum and maximum acquisition limits as well as calibration parameters Once the DAQ parameters are set sampled data files will assume the format established in the DAQ set up Thus
10. e Amp Period ASCII File frmMain vb mnuToolsGenAmpPeriodFile Create data file in txt for Convert Old Format to New Format frmMain vb mnuToolsConvertOldToNew Create txt file for Help nko 3 mme aw Nov 17 2005 by Joan Figure 2 Coding structure plot for NeoSuck RT 1 3 Program installation l Uninstall any previous installed versions of NeoSuck RT using Control Panel Add Remove Programs 2 Install NeoSuck RT by running setup exe from the installation package NeoSuck RT CNL U of Kansas Figure 3 BNC 2090 Note Normally it works with BNC 2090 interface Figure 3 To work with the BNC 2110 Figure 4 please make the following change Figure 4 BNC 2110 For BNC 2110 To work with BNC 2110 the terminal configuration has to be in differential mode Terminal configuration can be set to differential mode by modifying the parameter value of terminalConfig to 1 under lt systemConstants gt section in the file NeoSuckRT exe config located in C Program Files Neuro Logic NeoSuck RT Here is the line from NeoSuckRT exe config that shows this parameter value lt add key terminalConfig value 1 gt terminalConfig can have the following values NeoSuck RT CNL U of Kansas value 1 for Differential value 2 for Referenced single ended value 3 for Nonreferenced single ended By default the file NeoSuckRT exe config contains value 3 for terminalConfig This setting 1s used for
11. earch 2141 Seibel L Barlow S M Vantipalli R Finan D Urish M Carlson J 2005 Spectral Dynamics of Non Nutritive Suck in Preterm Infants Society for Pediatric Research 2139 Stumm S Barlow S M Vantipalli R Finan D Estep M Seibel L Urish M amp Fees M 2005 Amplitude Burst Dynamics of the Non Nutritive Suck in Preterm Infants Society for Pediatric Research 2632 Zimmerman E Barlow S M Seibel L Poore M Stumm S Estep M Chu S Fees M Urish M Gagnon K Cannon S Carlson J 2006 Pacifier stiffness alters the dynamics of the suck central pattern generator Society Pediatric Research 5571 393 Stumm S Barlow S Vantipalli R Finan D Estep M Seibel L Urish M Fees M Poore M Chu S Zimmerman E Cannon S Gagnon K Carlson J 2006 Amplitude Burst dynamics of the non nutritive suck in preterm infants J Clinical Nursing under revision Estep M Barlow SM Stumm S Fees M Finan D Seibel L Poore M Cannon S 2006 Non nutritive Suck Burst Parametrics 1n Preterm Infants with Respiratory Distress Syndrome Biol Neonate submitted 18
12. first column NeoSuck RT can automatically recognize the peak in suck pressure which is marked by small diagonal red lines in suck pressure plot 2 2 2 Fourier transform of suck pressure NeoSuck RT can be used to calculate the FFT of suck pressure In order to get the frequency property of suck pressure the user can click Tools Suck FFT to call up the FFT Suck Pressure window In this analysis window the user will find the time domain and 12 NeoSuck RT CNL U of Kansas frequency domain plot of the currently displayed block in the main NeoSuck RT window Figure 10 The first and second graphs are both for current block while the third graph named as composite FFT shows the mean FFT for all the blocks that have been analyzed by the FFT subroutine User could either click Update FFT Plot Save FFT button or go to Tools Generate Composite in this window to periodically update the composite FFT d NeoSuck RT C Program Files Neuro Logic NeoSuck RTWata K6_033004 K6_033004 dat pro FFT Suck Pressure Block 5 MOTOF Settings Tools SUCK PRESSURE cm H20 ACHO Suck Pr cm H20 Time s 3 0 FFT SUCK PRESSURE cm H20 ACHT FEBS ika Amplitude cm H20 i i 0 0 5 0 10 0 15 0 Frequency Hz FFT Suck Pr File K6_033004_B5 dft ACH2 FFT Suck Pressure Update FFT Plot Save FFT Amplitude 0 885 Frequency 0 046 Hz 0 05 1 SUCK PRES COMPOSITE FFT SUCK PR
13. n also be exported to txt file by clicking Tools Generate Histogram Files for future analysis Those files will be saved under C Program Files Neuro Logic NeoSuck RT Data 11 NeoSuck RT CNL U of Kansas NeoSuck RT C Program Files Neuro Logic NeoSuck RTWata K6_033004 K6_033004 dat File Settings Tools Help Select Baseline Amplitude Bad Block List Pause Period EMG DOS R uv Suck FFT Burst Period Create ASCII Blocks 305 52 Generate Histogram Files o Generate Amp Period ASCII File E 5 Convert Old Format to New Format 5 0 0 zem 200 0 LYDT mm EMG OOS L uv ism 50 04 EE u a 5 0 0 E x 1 5 50 0 LOAD CELL N EMG OOI R uv 50 0 0 154 e ceo 5 5 0 0 u 0 13 E 0 05 4 i i i I 50 0 i i SUCK PRESSURE cm H20 EMG OOI L uv 20 0 100 0 e m q z x 10 0 Q 0 0 3 0 0 1 1 l l I I 0 0 10 0 20 0 30 0 0 0 10 0 20 6 30 0 Time s Time s Block NeoSuck RT Block No V Real Time i 3 m Previous First Last Block 3 5 Peak Count 23 e Date Created 3 30 2004 1 53 32 PM Figure 9 NeoSuck window without Histogram analysis Similar to the recording process NeoSuck RT analyzes data in successive blocks 30 seconds for each block User can scroll among different blocks by clicking the Previous and Next buttons in the lower part of the window as shown in Figure 9 NeoSuck RT is mainly for suck pressure analysis which is shown as the last graph in the
14. the BNC 2090 signal interface Here is the line from NeoSuckRT exe config that shows this parameter value add key terminalConfig value 3 gt NeoSuck RT CNL U of Kansas 2 User manual When opening the NeoSuck RT a blank window is shown as follows Figure 5 User could apply this set of software and hardware to record new data or analysis of existing data ES NeoSuck RT fe fe x File Settings Tools Help MOTOR PULSE v EMG OOS R uv 10 0 10 0 ce 5 5 0 5 5 04 x E 10 0 x 5 5 5 0 F E i I o E m 0 04 l l l I LOAD CELL N Z 0 4 O 5 0 6 10 0 T T T T Amplitude cm H20 N 5 5 04 a 2 2 E D 2 e 10 0 T 0 5 I 5 gt 0 ur a Pause s 0 07 1 1 I l f 0 0 2 0 4 0 8 0 10 0 Time s L2 5 E V NeoSuck RT S N Real Time l 0 4 Period s Block PeakCount e Date Created Figure 5 Blank window for recording and analysis 2 1 Record new data by NeoSuck RT To Record data for a baby go to File New then a Save As file dialog will pop up as shown in Figure 6 Input the file name as dat file which will be used for future analysis and click Save button NeoSuck RT za NeoSuck RT C Program Files Neuro Logic NeoSuck RTWata test TestingA 061206 dat File Settings ols Help CNL U of Kansas MOTOR PULSE v Save As

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