Mass Profiler Pro Quick Start F8.book
Contents
1. see Documents on page 3 guides you through importing and organizing the Malaria Demo data Flow Chart of the MS Experiment Creation Wizard Step 1 Select Data Source Experiment Grouping Sample Summary Step 10 Normalization Criteria Step 2 Select Data to Import Steps 3 amp 4 skipped Step 11 Baselining Options Create an initial analysis Step 5 Sample Reordering Goto Step Figure 14 MS Experiment Creation Wizard Perform your initial differential analysis using the Analysis Significance Testing and Fold Change workflow The Significance Testing and Fold Change Wizard guides you through the steps necessary to enter parameters and values that improve the quality of your results and produce an initial differential expression for your analysis The Significance MPP Quick Start Guide 15 16 Testing and Fold Change Wizard flow diagram is illustrated in Figure 15 The MPP Familiarization Guide see Documents on page 3 guides you through an initial differential analysis using the Malaria Demo data Flow Chart of the Analysis Significance Testing and Fold Change Wizard Step 7 Fold Change Step 8 Experiment Quality Control on ID Browser Grouping Samples Identification Significance Save your project and Analysis perform advanced tasks Goto Step Figure 15 Analysis Significance Testing and Fold Change Wi2. 009 ee Agilent G3835AA MassHunter 009 Mass Profiler Professional Software i i Quick Start Guide Getting Started 4 User Interface 8 Basic Tasks 12 Advanced Tasks 21 What is Agilent Mass Profiler Professional Agilent Mass Profiler Professional MPP software is a powerful chemometrics platform designed to exploit the high information content of mass spectra MS data and can be used in any MS based differential analysis to determine relationships among two or more sample groups and variables MPP provides advanced statistical analysis and visualization tools for GC MS LC MS CE MS ICP MS and NMR data analysis MPP also integrates smoothly with Agilent MassHunter Workstation Spectrum Mill and ChemStation software and is the only platform that provides integrated identification annotation of compounds and integrated pathway analysis for metabolomic and proteomic studies The system also enables Automated Sample Class Prediction that revolutionizes mass spectrometer based qualitative analysis of unknown samples in many applications MPP is ideally suited for applications characterized by complex sample matrices such as metabolomics proteomics natural products food beverages flavors fragrances and environmental analyses os Agilent Technologies Features of Mass Profiler Professional Where to Allows differential analysis of two or more sample sets from one or multiple MS analysis platforms in a single project Desi
3. The Agilent Malaria Demo data supplied with MPP is used as an example in each step You may substitute the malaria information with information for your data Steps Detailed Instructions Comments 1 Start Mass Profiler Professional a Click the Mass Profiler Professional e The Startup dialog box helps you icon ajos your desktop or click quickly set up a new project this Start gt All Programs gt Agilent gt section or continue your analysis MassHunter Workstation gt Mass with a previous project see Open Profiler Professional gt Mass Profiler a recent or existing project on Professional page 1 2 Create a new project from the a Click Create new project e Create new project provides you Startup dialog box b Click OK with the option to create a new experiment or import an experiment x from an existing project into the Welcome to MassProfiler Pro new project Select what you would like to do from the options below then dick on OK e After closing an open project you may create a new project from the Menu bar click Project gt New Project or from the Toolbar click the New project button Select recent project New Project FF Do not show this dialog again Help 12 MPP Quick Start Guide Steps Detailed Instructions Comments 3 Type descriptive information inthe a TypeMalaria ProjectinName New Project Details section ofthe b TypeProject containing the The project
4. jeriment Setup a Jick Start Guide periment Grouping ate Interpretation uality Control on Samples ter by Frequency ter on Sample Variability ter by Flags ter by Abundance ter by Annotations alysis a atistical Analysis ter on volcano Plot ld Change ustering nd Similar Entities ter On Parameters incipal Component Analysis nd Minimal Entities gt ss Prediction jild Prediction Model in Prediction in Prediction From file port Prediction Model sults Interpretations a nd Similar Entity Lists port for Recursion Browser Identification port for Identification port Inclusion List port Annotations thway Analysis ngle Experiment Analysis ulti Omic Analysis unch IPA port to MetaCore ammect to Cytoscape gt gt Networks P Network Discovery 25H Network Builder tract Relations via NLP lities move Entities with missing signal values alysis Significance Testing and Fold Change ass Prediction Build and Test Model ure 11 Workflow Browser operations Workflow Browser The Workflow Browser Figure 11 left is organized into sequential groups of operations for the analysis of your data Experiment Setup Quality Control Analysis Class Prediction Results Interpretations Pathway Analysis optional NLP Networks optional and Utilities Legend The Legend Figure 12 shows the key scale to the use of color in the active desktop view Right
5. Advanced Tasks on page 21 Review the MPP Familiarization Guide see Documents on page 3 This guide helps you quickly create a project create an experiment import and organize data and create an initial differential expression using pre installed demonstration data Malaria Demo Review the MPP Application Guide see Documents on page 3 This guide provides you with more detail about the MPP software and helps you create a project create an experiment import and organize data and create an initial differential expression with your own data Perform advanced analyses to meet your project needs using the operations available in the Workflow Browser see Workflow Browser on page 11 MPP Quick Start Guide License Management Activating a License The first time you start MPP you enter your Order ID License activation information is entered in the Mass Profiler Professional License Activation dialog box shown in Figure 1 An internet network connection is required Mark Mass Profiler Professional Order ID If you have additional software product licenses mark your additional products Enter your Mass Profiler Professional Order ID If you have additional software product licenses enter your additional product Order ID s 1 Hais Proliler Profession Lienee Activation E Click OK The Mass Profiler Professional software automatically validates your license and start Mass Profiler Professiona
6. name and notes may be viewed and edited at any time using Create New Project dialog box Agilent Malaria Demo in the Project Inspector by clicking Notes Project gt Inspect Project from the c Click OK menu bar x x New Project Details mNew Project Details 7 Name MEE Name Malaria Project Notes Project containing the Agilent Malaria Demo re ox cono 4 Create a new experiment in the a Click Create new experiment Experiment Selection Dialog b Click OK dialog box x An experiment is an organized collection of LEMS or GCIMS sample data from a given data source IF you have an experiment you wish to use From 4 previous project please choose Open existing experiment You may also create a new experiment with new data or previously imported data Choose Experiment f Open existing experiment You may create or add a new experiment to your project from the Menu bar click Project gt New Experiment or from the Toolbar click the New experiment button or click the Add experiment it If you click Open existing experiment you are prompted for the experiment to add to the project as described in Open a recent or existing project on page 17 MPP Quick Start Guide 13 Steps 5 Type and select information that a guides the experiment creation in the New Experiment dialog box Detailed Instructions Type the descriptive name Malaria demo in Experiment name Thi
7. 2013 Genaionng Hab FEA Llid Ho Lidl Pallai bei hbert Expira On iter SOLS Expres On 15 Mar 20015 ox Figure 3 License Manager dialog box 3 Mark Mass Profiler Professional and any additional product Order ID s to surrender 4 Click OK DOO Choose the prodecig orde De to nerender Choose Product Fe Hass Profiler Professional Fer epr E F Paha Arcibect Fes Figure 4 Order ID surrender product selection dialog box 5 Click Yes in the Confirm Surrender dialog box Because your license is no longer active on the current computer MPP software immediately closes when you click OK MPP Quick Start Guide e That apirilan allows pos Lo cis Lhe appbeation with fhe pene Onder d on smother compraber live Toa Hive Toi Wini A Nou Pir pii Waal lo Parteners the borde an ae Figure 5 Confirm Surrender dialog box 6 Click OK in the Automatic Surrender Operation Successful dialog box Because your license is no longer active on the current computer MPP software immediately closes x O Your kenge has been purerdered sunset gt Figure 6 Automatic Surrender Operation Successful dialog box 7 Activate your license Order ID on the new computer see Activating a License on page 5 8 Optional Click Start gt All Programs gt Agilent gt MassHunter Workstation gt Mass Profiler Professional gt Uninstall to remove MPP from the current computer MPP Quick Start Guide 7 User Interface Note
8. Help and detailed information regarding the various fields and statistical treatments are available by pressing F1 or by referring to the Mass Profiler Professional User Manual Main Functional Areas The main functional areas of Mass Profiler Professional are shown in Figure 9 on page 9 The main MPP window consists of four parts 1 the Menu Bar 2 the Toolbar 3 the Display Pane and 4 the Status Bar 1 Menu Bar The menu bar Figure 7 provides actions that are used for managing your projects experiments pathways and display pane views gt Project Search View Tools Annotations Windows Help Figure 7 Menu bar 2 Toolbar The toolbar Figure 8 is located below the menu bar and contains five sections of buttons for commonly used tasks Project section New project Open project and Close project Experiment section New experiment and Add experiment Entity List section Create entity list from selection Inspect selected entity and Import entity list from file Plots Statistics and Pathways section Scatter plot Profile plot Histogram plot Matrix plot Venn diagram plot Box and whisker plot Heat map plot Data spreadsheet Summary statistics Create new pathway and Select data source for plots Sidebar section Hide or show the sidebars that contain the Project Navigator Workflow Browser and Legend Project Experiment Entity List Plots Statistics and Pathways irren 886 SS GRD PS0 26 SeeaRs Figu
9. Open recent project go to Step 2 Open existing project go to Step 3 Create new project go to Step 4 and import an experiment from a previous project Open recent project opens the project and the experiment s that are stored in the project If your project is not shown in the Select recent project list go to Step 3 and select it from the list of existing projects on your computer A project is a container for a collection of experiments A project can have multiple experiments on different sample types and organisms You may now use the operations available in the Workflow Browser MPP Quick Start Guide 17 Steps Detailed Instructions Comments Open existing project a Click Open existing project in the e 3 Open an existing project in the Startup dialog box in Step 2 on Startup dialog box and select the page 17 Malaria project from the list of all b Click OK e of your existing projects c Select the Malaria project row from the Open Project dialog box d Click Open e x Modified Date Owner Malaria SatMay2114 1 Sat May 21 14 1 Displaying results from 1to 1of 1 Showingpae 9 fi a of a He open cora e 18 Open existing project allows you to open a project that is no longer displayed in the recent project list Open existing project opens the project and the experiment s that are stored in the project When you have a large number of existing projects on your computer you ma
10. click on the Legend window to copy and export the legend as described in the Desktop area Legend Profile Plot Color By 3 4_pH7_pos_01 lg2 19 6 0 1 20 9 y Figure 12 Legend 4 Status Bar The status bar Figure 13 has three informative areas the Status Area the Ticker Area and the Memory Monitor Status Area Displays high level information of the current view such as the number of rows and columns in table views and the number of entities or conditions selected in plot views Ticker Area Displays the coordinates of the cursor in active plot views or the entity identification and value in table views Memory Monitor Displays the total memory being used and the total memory allocated by MPP You can click the Garbage Can icon if at any time to reduce the memory usage H Status Area I Ticker Area Memory Displaying 941 9 selected 15 05 3 4 pH7_pos_ esMmofiosh Figure 13 Status Bar MPP Quick Start Guide 11 Basic Tasks Set up a new project A project is a container for a collection of experiments each experiment contains your samples interpretations and analyses A project can have multiple experiments on different sample types and organisms Create a new project and experiment for your data A new project and experiment is created through four sequential dialog boxes 1 Startup 2 Create New Project 8 Experiment Selection and 4 New Experiment
11. ect the Malaria LCMS ESI pH 7 experiment in the Add Experiment dialog box h Click Add add Experiment Malaria LCMS Sh a sl pH 7 Ma y ser Dec 19 113 Dee 19113 puser Masser Differentiation by Infec Fri Jun 22 15 Fri Jun 22 15 41 Differentiation by Infec Fri Jun 22 17 Fri Jun 22 17 52 Displaying results From 1 to 7 of Showing page Q fi O of i Help Cancel Cancel The selected experiment is shown highlighted using a background color The Malaria LEMS ESI pH 7 experiment in the Malaria project is now imported into your new project and appears as if it was opened using Open recent project You may now use the operations available in the Workflow Browser MPP Quick Start Guide Save your project Save your current analysis as a TAR file for archiving restoration of any future analysis to the current results sharing the data with a collaborator or sharing the data with Agilent customer support Steps Detailed Instructions 1 Export your project to a TAR file a Click Project gt Export Project Elmass Profiler Professional Differentiation by Infection and Treatment Project Search View Tools Annotations Windows Help New Project al RO h O A Open Project Recent Projects x Import Project Inspect Project nd Treatment Close Project Delete Project Share Project Export project as tar archive Change Project Owne Refresh Project New Exp
12. eriment Add Experiment ned Exit Ctrl b Mark the check box next to the experiment you wish to save c Click OK OATES Experiments x Select experiments to export with the project All the contents of the experiment will be exported and can be imported later Sequencing experiments and MOA experiments with a sequencing experiment as input are disabled and cannot be exported JV Differentiation by Infection and Treatment _ Help Cancel d Select or create the file folder e Type the File name f Click Save Comments 20 MPP Quick Start Guide Advanced Tasks The operations available in the Workflow Browser see Figure 11 on page 11 provide the tools necessary for analyzing features from your mass spectrometry data depending upon the need and aim of the analysis the experiment design and the focus of the study This helps you create different interpretations to carry out the analysis based on the different filtering normalization and standard statistical methods It is recommended that you follow the procedures in the MPP Familiarization Guide and MPP Application Guide see Documents on page 3 for increasingly detailed information to help you use MPP The Discovery Workflow Guide and the Mass Profiler Professional User Manual see Documents on page 3 provide you with techniques and explanations to perform advanced analysis tasks MPP Quick Start Gu
13. gned for both mass spectrometrists and statisticians with guided and advanced workflows Provides comprehensive statistical tools including ANOVA PCA volcano plots hierarchical trees SOMs QT clustering and five different methods for class prediction Generates inclusion lists for subsequent MS MS based analyses to facilitate identification of compounds of interest The ID Browser allows integrated identification and annotation of compounds using LC MS Personal Compound Databases METLIN Pesticides Forensics and GC MS libraries NIST and Agilent Fiehn Metabolomics Allows import and export of data in several formats e g to further extend and customize statistical analysis and visualization capabilities using R Scripts Integrates smoothly with Spectrum Mill to enable TOF Q TOF based discovery and QQQ based verification of protein biomarkers Integration with GeneSpring allows the correlation of pathways between MS metabolomics proteomics and microarray data transcriptomics in one project enabling true integrated biology studies Class prediction models from GC MS LC MS CE MS and ICP MS data now can be used with the new standalone Automated Sample Class Prediction software to enable high throughput routine classification of unknown samples Find More Information Online help Press F1 To get more information about a pane window or dialog box place the cursor on the part of the pane window or dialog box of intere
14. ide 21 22 BioCyc Pathway Genome Databases Includes BioCyc Pathway Genome databases from the Bioinformatics Research Group at SRI International used under license http www biocyc org Citation based on use of BioCyc Users who publish research results in scientific journals based on use of data from the EcoCyc Pathway Genome database should cite Keseler et al Nucleic Acids Research 39 D583 90 2011 Users who publish research results in scientific journals based on use of data from most other BioCyc Pathway Genome databases should cite Caspi et al Nucleic Acids Research 40 D742 53 2012 In some cases BioCyc Pathway Genome databases are described by other specific publications that can be found by selecting the database and then going to the Summary Statistics pages under the Tools menu The resulting page sometimes contains a citation for that database MPP Quick Start Guide MPP Quick Start Guide 23 www agilent com In this book The Agilent G3S35AA MassHunter Mass Profiler Professional Software Quick Start Guide presents the first steps to use the MassHunter Mass Profiler Professional Software Agilent Technologies Inc 2012 Revision A November 2012 G3835 90009 fit Agilent Technologies
15. l select License Typa PiasEe thotaa you canes ppe ibidu carre Ordear IO T u Shi Fret vild Onder lo Seite M s Probe Prol sional detr he wee Mass Profiler Professional Order lb en Spring GX Orderl Cenespring HGS OnderiD Palta chica Or ier E Example Amt TIBOO Poon el lies Usa proxy OK Cancel pel Ordenis wie lo he suppor bese al informales _ supporiGagilent com Figure 1 Moving a License Mass Profiler Professional License Activation dialog box MPP licensing is automatically enforced You may move a license to another computer by surrendering it on the current computer and then activating it on the new computer If you need to un install MPP it is always best to surrender the existing license before un installing MPP so that the license is available to be moved to another computer MPP Quick Start Guide To surrender your license 1 Click Help gt License Manager in the menu bar Contact Trata Speen C LA Updlste Product t Aree Figure 2 License Manager is available from the Help menu 2 Click Surrender in the License Manager dialog box x daer 1 ivailablerT tal Samples Mass Proba Protessiorak 154 1141 54 uaren UMUIMITED GencSpring Ge 11541341354 CET LIMITED Pathway Ar checi 1 ies mammen LALIHITED Modules Mica hue Liner Salus Lpabobes latir Pela Profi Prol etal Expres On ITA LE Expres On ai Genetpring GX Expres On 12 Epr 2013 Expres Om 15M
16. on G3335 90147 Revision A October 2012 Quantitative Analysis Familiarization Guide Agilent MassHunter Workstation Software Quantitative Analysis Agilent publication G3335 90061 Fourth Edition April 2010 Training Road Show Slide Presentation Metabolome Analysis From Sample Prep Through Data Analysis Metabolomics Road Show March 2011 Training Courses Visit www chem agilent com to view a listing of training courses for MPP MPP Quick Start Guide 3 Getting Started How do get started This Quick Start Guide helps you launch MPP activate your license review the MPP user interface and create a project and an experiment that you import data into and then use to begin your analysis If you are already familiar with the MPP software after you install MPP you can immediately start using the preloaded demonstration project called Malaria Start and Explore Mass Profiler Professional 1 Double click the Mass Profiler Professional icon located on your desktop or click Start gt All Programs gt Agilent gt MassHunter Workstation gt Mass Profiler Professional gt Mass Profiler Professional Activate your license The first time you start MPP you are prompted to enter your Mass Profiler Professional Order ID before you can use the software see Activating a License on page 5 Review the following three sections in this Quick Start Guide User Interface on page 8 Basic Tasks on page 12
17. pane see Figure 9 on page 9 is further divided into five areas the Project Navigator the Experiment Navigator the Desktop Area the Workflow Browser and the Legend Project Navigator Displays the current project and lists all the experiments with the project Experiment Navigator Displays information related to Samples Interpretations Analysis and My Favorites in folders related to the selected experiment in the Project Navigator Each experiment within a project has a separate experiment navigator window Desktop Area Display of one or more interactive views associated with the experiments Each view in the desktop area is marked with a tab and you can configure each view separately Click Windows gt Tile to arrange multiple views on the desktop The current experiment interpretation and analysis is noted using bold font in the Project Navigator and Experiment Navigator A right click anywhere in the active view shows you a menu of options Figure 10 to customize the view copy the view to the system clipboard and export the view as an image file bmp jpg jpeg png and tiff or as an html file Select Al Rowe Inwert Foca Selection Clear Row Selection Lidl Ta Ao Sitka Benet Piers Freeze Columns Before Properties OhrbeF Erpat As Properlics Cira Figure 10 The right click available options within the desktop view automatically adjust to the type of view 10 MPP Quick Start Guide
18. re 8 Toolbar MPP Quick Start Guide SulULId 10 oged y UO pote OI ST 3INSIJ STU 220N 9JEMIJOS ddIN 24 JO SeaJe euonouny uiew ay G auNnfily papayas p Tiei Banari 3 PO p p pue prg agg miiy 4 pd tury Bo posa Dep y ARA pi LA birdani wy na jr E spe a Se ae eee rte E x PEE MMT MOS bE UOTE WHEE MOTTE Ue eT OF EE NEL Al a a a sm abesn Aowa aonpal 07 ues abequeb ayap oyo Uowaw e pue MaM UAUND aly o payejas uonewojuj 571 000 PR 11 Ag a ocre te at pater s oping i i i Mall yuana aul AA B RAE z n z i I j J 1 i y p rica i gt ME j F hie E b 4 m miang pr afer Fay He i E lis anig Ae iia ME T Phan ssi 5 ga W Wi Di quawuedxe pajdajas ayy mena anne wai 7 Sue p e E HE e upm sazuoney pue sasAjeue ras som pes pas 7 Y W mi j NN Ai i N y viy suoneasdiejui sajdwes ssn mente D Y E i baud Aa ta a el i j ee pr A y AE AO AS AR yasmolg Mopp BU pue i m gas De dao anoe paari L E Behe hk zy Jonen uawnadxg ay ur apew cum Boe E ee i nee suonsaas Jo smar aanaesayu Pe f minita A W ao sn A Load uo area W 7 y y i pe oid gan Y de Y BEEN cal mre nm Ez AS AMNA f A me RA wipers y W V se rome erp PA i MAN ae H N i NV by TERN Juano ayy a E AI unam sjuawuadxe ajos io AMA 101e61ne y 1991014 remeros ME AA A RE a mT mi ni a i aleg ejdsig MPP Quick Start Guide 3 Display Pane The display
19. s name may be different from the project name Comments Experiment type determines how Mass Profiler Professional manages the data Unidentified is the proper b Select Mass Profiler Professional for Experiment type selection when Analysis type the compounds have only been c Select Unidentified for the Experiment identified by their molecular type features of neutral mass and d Select Analysis Significance Testing retention time and Fold Change for Workflow type Identified is the proper Experiment e lypeMalaria LCMS ESI pH 7 type selection when the in Experiment notes compounds have been identified by f Click OK compound formula and or CAS number e Combined Identified Unidentified is the proper Experiment type when you are unsure if the data has been identified in full or in part or when MassHunter Qualitative Analysis has been previously used to identify some of the compound features Mew Experiment 22222 xl x Experiment description Experiment description Enter a name analysis type experiment type and a desired workflow type Analysis will guide you through a statistical significance test and fold change analysis Data Import will guide you through experiment creation only Class Prediction will guide you through the creation and testing of a prediction model using imported training data Experiment name ENEE Analysis type Expression si iti O C SY E
20. st and press Fl Help Menu Click Help gt Documentation Index to access the release notes quick start guides and HTML and PDF versions of the Mass Profiler Professional User Manual MPP Quick Start Guide Documents MPP Familiarization Guide Agilent G3835AA MassHunter Mass Profiler Professional Software Familiarization Guide Agilent publication G3835 90010 Revision A November 2012 MPP Application Guide Agilent G3835AA MassHunter Mass Profiler Professional Software Application Guide Agilent publication G3835 90011 Revision A November 2012 Mass Profiler Professional User Manual Agilent MassHunter Mass Profiler Professional User Manual Agilent publication January 2012 You can find a PDF copy of the user manual in the MPP installation folder C Program Files Agilent MassHunter Workstation Mass Profiler Professional docs manual Discovery Workflow Guide Agilent Metabolomics Workflow Discovery Workflow Guide Agilent publication 5990 7067EN Revision B October 2012 Discovery Workflow Overview Agilent Metabolomics Workflow Discovery Workflow Overview Agilent publication 5990 7068EN Revision B October 2012 Qualitative Analysis Familiarization Guide for LC MS Agilent MassHunter Workstation Software Qualitative Analysis Agilent publication G3335 90146 Revision A October 2012 Qualitative Analysis Familiarization Guide for GC MS Agilent MassHunter Workstation Software Qualitative Analysis Agilent publicati
21. xperiment type Jagilent Expression Two Color YS Workflow type analysis Biological Significance i Experiment notes Cancel Help 14 Enter a name analysis type experiment type and a desired workflow type Analysis will guide you through a statistical significance test and fold change analysis Data Import will guide you through experiment creation only Class Prediction will guide you through the creation and testing of a prediction model using imported training data Experiment name TE Analysis type Mass Profiler Professional SS Experiment type Unidentified amp f Workflow type Janalysis Significance Testing and Fold Change y Experiment notes Malaria LEMS ESI pH 7 Cancel Help e Your new project is now set up e You are immediately guided through the steps to import and organize your data followed by the steps to create an initial analysis MPP Quick Start Guide Import your data using the MS Experiment Creation Wizard After you set up a project and create an experiment the MS Experiment Creation Wizard guides you through the necessary steps to organize your experiment import your data define your experiment variables and prepare your data for analysis The MS Experiment Creation Wizard flow diagram is illustrated Figure 14 The data preparation includes grouping filtering alignment normalization and baselining The MPP Familiarization Guide
22. y have to use the page navigation buttons in the Open Project dialog box A selected project is highlighted by a color based on your PC s system settings You may now use the operations available in the Workflow Browser MPP Quick Start Guide Steps Detailed Instructions Create new project a Click Create new project in the e 4 Create anew project in the Startup Startup dialog box in step 2 on dialog box and import the page 17 experiment stored in the Malaria b Click OK project into the new project c lypeMalaria Project in Name d Type Import Experiment in Notes e Click OK x x New Project Details New Project Details Comments Create new project allows you to import an experiment from an existing project into the new project See Set up a new project on page 12 for creating a new project and a new experiment Notes gt Notes Name Malaria Project Import Experiment Help Help f Click Open existing experiment in the Experiment Selection Dialog dialog box experiment Selection Dialog An experiment is an organized collection of LEMS or GCIMS sample data from a given data source IF you have an experiment you wish to use From a previous project please choose Open existing experiment You may also create a new experiment with new data or previously imported data xl Choose Experiment Create new experiment g Sel
23. zard MPP Quick Start Guide Open a recent or existing project When you start Mass Profiler Professional the Startup dialog box allows you to quickly open a recent project an existing project on your computer or import an experiment from an existing project into a new project The steps presented are recommended if you are already familiar with the operations available in the Workflow Browser If you are new to using Mass Profiler Professional start with Set up a new project on page 12 and then refer to the MPP Familiarization Guide see Documents on page 3 Steps Detailed Instructions 1 Start Mass Profiler Professional a Click the Mass Profiler Professional icon ajos your desktop or click Start gt All Programs gt Agilent gt MassHunter Workstation gt Mass Profiler Professional gt Mass Profiler Professional b Go to Step 2 Step 3 or Step 4 Open recent project a Click Open recent project 2 Open a recent project in the b Select Malaria from the Select recent Startup dialog box and select the project list Malaria project from the recent c Click OK project list x Welcome to MassProfiler Pro Select what you would like to do from the options below then cick on OK Options Create new project Malaria FF Do not show this dialog again e Comments MPP begins with the Startup dialog box to help you quickly open a previous project
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