RapidWash - Hudson Robotics
Contents
1. Rapid Was User Manual Rev 1 2 Z ludson ROBOTICS INC 10 Stern Avenue Springfield NJ 07081 Tel 973 376 7400 Fax 973 376 8265 SECON Mecca 6 ene A ars Teter RO O Osea O ae Ty RE NOTR 3 bls Dubin sand COMME CUONS aii ele ie ke E A 3 Mp ee E tienen yuietiets dag O S RO O RA VA 4 t22 Rapid W asi Select Man Old ano ea a Sre pekle 5 kije TONOM e a ed uaseatiaihaneiseanaies Error Bookmark not defined Section 2 Operation of RapidWash through the Touch Screen Controller 7 obeske Pad Func MONS a ean eee nae 7 DRE EAS RRR E Cee Mee TCR tT set RR en eter A eee NO VNO 8 Dae eae GA O oom ae E O A A Rene Meme ne O noe nT meen ene TS 12 ENO PAME ses O O O O lara bared haa aaa anne AN O 13 ZS ia mte mane NOC aeaaee EEE E AE TORN ia 14 2 O GANGS S U O S aa e a en ola ae bele nede nan 17 Section 3 Maintenance of the RapidWash Manifold ec eccccccccccesesseeeeceeeeeeeeeeeeeeees 23 3 1 Removal and Installation of the Manifold eecccccccccccceeesseeeeeeeeeeeeeeeeeees 23 9 2 Disassembly Or the Mam old na A A bag ei ke 25 2 eo GH o E A V VE V NA VERA TV PA RE RA TEA 29 Section 4s Hard Ware Specifica OnS sseredcspsessei eia T S 26 SEO E E GCC Ta EN A AA O S D E 26 Secon 42e Mechanical Descrip oN ae aa n Zabave eki 26 Secon hI A fe a A A A A ET A V nna TNI 26 SECUION A a DIMENSIONS aa a ee bin ai da a ive bine dok j ka 27 DECOM AE ea oe RA A A A IE AR EOR AME NA O RE ONE NJA 21 Secon Se En2. Dispense Select the liquid number 1 or 2 and the desired volume in uL Figure 12 Note If the liquid 1 or 2 being dispensed has not been primed into the dispense head the RapidWash will automatically perform a priming step prior to the first dispense step in the Wash Program being run Subsequently if only that liquid is dispensed in that Wash Program the remaining dispense steps will execute without another priming action Figure 12 Delay Entered as seconds During a delay the plate is moved away from the manifold and the washer will not aspirate or dispense until the delay is over Figure 13 10 Add Delay Step Figure 13 Example of Wash Program Figure 14 Wash Program 01 Aspirate 10 75 mm down at 50 s Dispense 150 uL of Liquid 1 Aspirate 10 75 mm down at 50 Dispense 150 uL of Liquid 1 t A Aspirate 10 75 mm down at 45 spee Figure 14 All program values and steps are automatically saved to that program as they appear on the screen in Figure 14 11 2 3 Prime The Prime feature allows you to select the liquid number and the total volume in uL dispensed through the dispense manifold Priming occurs at the priming trough During the prime process the trough will self empty to the waste bottle Figure 15 E w H fi Figure 15 Volume This is the total volume that will be primed through the system Liguid This allows you to select the fluid sourc
3. mount until it locks into place Figure 29 To remove the manifold press the Steel release tab on the top of the quick disconnect then slowly slide the manifold off the locating pins on the manifold mount Figure 29 Figure 29 24 3 2 Disassembly of the Manifold Remove the flat head screws that hold the aspirate and dispense manifolds together If desired the standoffs may be removed from the dispense manifold Figure 30 Figure 30 3 3 Cleaning All of the pump parts may be cleaned in an ultrasonic cleaner with a dilute detergent solution The parts must be thoroughly rinsed prior to reassembly 25 Section 4 Hardware Specifications Section 4 1 General NOTE Specifications are subject to change without notice Rapid Wash e Plate Format Housing Material Plate Nest Material Number of Axes Section 4 2 Mechanical Description e Y axis Y axis Y axis Travel Distance Y axis Stepper Motor Z axis Z axis Travel Distance Z axis Stepper Motor Section 4 3 Materials Housing Material Reservoir Material Valve Material Tubing Material 96 384 well plates in shallow medium or deep well formatted SBS plate Painted steel covering anodized aluminum housing Acetal Copolymer Two Y and Z axes cast Integrated stepper motor stepper controller with encoder This is a belt driven axis with pulley Stepper motor linear actuators with 250 count encoder Actuator has
4. will not validate liquid 2 It is strongly recommended that the validation checks are not disabled Check Waste Level Checks if the sensor on the waste bottle is activated This may be deactivated for an in house vacuum and waste management system Check Liquid Level Checks if the sensor on liquid bottle detects fluid Check Liquid 2 Level Checks if the sensor on liquid bottle 2 detects fluid Check Dispense Pressure Checks if there is pressure for dispensing This may be deactivated for motion testing or demo purposes and will run without dispensing fluid Min Aspirate Vacuum Checks if there is vacuum supplied to the unit WARNING Disabling this check can lead to flooding 22 Section 3 Maintenance of the RapidWash Manifold 3 1 Removal and Installation of the Manifold The RapidWash is shipped without the manifold assembly attached to the unit The manifold assembly is shipped with a shipping bracket to protect the tips This shipping bracket must be removed before the Manifold assembly can be installed onto the RapidWash The Manifold assembly has a quick disconnect fitting that holds the Manifold Figure 27 Manifold Quick Disconnect Fitting Figure 27 The side of the manifold has two locating holes that allow the manifold to slide onto the two locating pins on the manifold mount Figure 28 23 Figure 28 Slide the manifold onto the locating pins and continue to slide the manifold towards the
5. 6 well plate or 100 uL in every well If run without the manifold attached volume dispensed will not match what is defined due to a different flow rate without the head On the run screen enter volume in uL and press Run Manual Move This feature is used to jog the dispense head Y Axis IN or OUT jog the plate nest Z Axis UP or DOWN and OPEN CLOSE the plate gripper on track based units Three settings are available for the distance of each jog movement 0 1mm 1mm or 10mm 16 2 6 Change Settings This Section allows you to modify all the performance settings of the RapidWash Figure 19 JE Dispenser Settings 7 ravel Shift Teaching Plate well Time Initialize on Powerup Set Dispense Pressure Prime Volume D Figure 19 Settings Tab Aspirate Shift This is the distance that the RapidWash will use above and in the plate wells to begin the aspirate step 4mm is above the top of the Plate and mm is aspirate begins in the plate Dispense Shift This is the distance that the RapidWash will use above and in the plate wells to begin the dispense step mm is above the top of the Plate and mm is aspirate begins in the plate Travel Shift This is the distance that the RapidWash will travel above the Plate when the manifold moves between Aspirate Dispense and Prime Positions mm is above the plate Teaching Plate This is the plate height of the microtiter p
6. ad Functions Wash Up to 20 Wash programs can be created and stored Prime Pumps either liquid 1 or 2 through the dispense manifold Auto Prime Allows the dispense path to be primed on a time interval Maintenance Mode Tools for cleaning dispense and aspirate paths Change Settings Define parameters for operation such as plate locations WSR1 01 FW Rev A 02 Figure 7 2 2 Wash The RapidWash can support up to 20 dispense programs Press the Next or Prev key Figure 8 on the Touch Panel to select the program number you would like to setup Mash Program 01 Aspirate 10 75 mm down at 45 spee Figure 8 Wells Select the well pattern of the plate used in this program by pressing the appropriate screen button Figure 8 Plate Height Enter the height of the plate used in this program in mm above the base of the plate nest see also Figure 9 New This will add a new Aspirate Dispense or Delay step in your Wash Program see also Figure 10 Move Up Allows you to move the position of the highlighted line up one line in the program Move Dn Allows you to move the position of the highlighted line down one line in the program Figure 9 Select Step Type Figure 10 Aspirate Allows selection of Depth The distance in mm traveled down into the plate wells measured from the top of the plate Speed of full speed travel during the aspirate step Figure 11 Figure 11
7. ch well of the plate Figure 23 Figure 23 Track Nest Height This Position should only be modified when the unit is used with a TrackLink on our Lablinx system This is the beginning position of the nest default is 0 When used with a track the position is taught just below the bottom the track Figure 26 Figure 24 Grip Position This position is only used when a nest with a gripper is attached to the RapidWash This is used for through track based washing only This is the Position at which a plate is gripped by the nest before the washing procedure This Position is usually taught 5mm above the track Figure 25 20 Figure 25 Plate Origin This is the starting position origin of the aspirate tips relative to the teaching plate To teach this position move the Aspirate head out until the front set of tips are aligned with the teaching plate Then move the nest up until the aspirate tips touch the top of the teaching plate Figure 26 titi i Mo umi a JEM m 7 Ma 4 Ni 4 4 sA 3 bt ziji lt a na m ee ee RD Mk ine o a ae a te wre tat dla vem PRESE S iki Lh ih MY me petna mao i s nos Seah fai Figure 26 21 Calibration tab The validation setting turn on off various checks that the unit can perform prior to executing programs and priming Validation checks occur only on the resources needed for a particular operation i e If liquid 1 is the only fluid being used the unit
8. e for the prime 12 2 4 Auto Prime Auto Prime performs exactly like the Prime feature but will run automatically at the user determined interval Figure 16 Volume This is the total volume that will be primed through the system Liquid This allows you to select the fluid source for the prime Time This 1s the interval in seconds a time of 0 deactivates this feature mo AutoPrime Figure 16 13 2 5 Maintenance Mode The features in this section are used to clean and maintain the performance of the dispense and aspirate manifolds Figure 17 Maintenance Figure 17 Cleaning Procedure This feature is used to flush the dispense manifold it can be helpful when attempting to remove debris from the dispense manifold During the cleaning procedure wash solution is dispensed out of the dispense manifold into a user supplied trough and aspirated back though the dispense manifold to waste A user supplied trough is required for this procedure It is placed in the plate nest prior to starting the procedure Clear lids for SBS pipette tip boxes work well and allow you to see the cleaning cycle during operation If a clean non system wash solution is desired the user supplied trough can be filled with the desired solution prior to the wash step The control parameters are as follows Figure 18 14 Figure 18 Plate Height Operates as described previously Cycles The number of times the cleanin
9. g process will be performed Volume This is the volume dispensed through the system because the fluid path could be empty after your first cycle you should pay close attention to this volume to avoid under or over dispensing into your wash plate Liquid 1 Liquid 2 Operates as described previously Soak Delay Number of seconds the dispense manifold will soak in the dispensed liquid Drain Time The number of seconds the Dispense manifold will aspirate to waste Empty Manifold This feature is used to pull vacuum on the aspirate manifold The unit does not move and only turns on the vacuum This feature is typically used when cleaning the manifold The manifold does not need to be mounted on the unit On the run screen enter time in seconds and press Run Empty Trough This feature is used to pull vacuum on the priming trough The unit does not move and only turns on the vacuum to the trough This feature is used to clear excess fluid from the trough On the run screen enter time in seconds and press Run Dispense Liquid This feature is used to dispense liquid from unit The unit does not move does not turn on any vacuum and only dispenses liquid at the current location This feature is used to quickly test dispensing functionality The net volume dispensed from the unit is exactly as defined in the step it is not defined 15 by the number of wells G e a volume of 9600 would lead to 9 6 mL dispensed across a 9
10. integrated 1 2 pitch Leadscrew Controlled by separate stepper controller with encoder option 211mm hard stop to hard stop 1000 encoder steps per revolution linear actuator 0 026mm resolution approximately second for entire travel distance Stepper motor linear actuator Actuator has integrated 9 4mm pitch Leadscrew Controlled by separate stepper controller 75mm hard stop to hard stop 200 steps per revolution linear actuator 0 006mm resolution approximately 1 2 second for entire travel distance Stainless steel covering anodized aluminum frame Polypropylene PTFE Acetal fittings on Tygothane Polyurethane Tubing 26 Manifold e Manifold Material e Sealing O rings e Fixed tip Material Section 4 3 Dimensions e 2 34 HX1111 W X I498 D e Weight 1OLBS Section 4 4 Electrical e Power Input e Fuses e Grounding e Computer Interface Section 4 5 Environmental Operating Humidity 6 e e Storage Temperature e Altitude Section 4 6 Pneumatic e Air Supply e Vacuum Operating Temperature Acetal Copolymer Viton Stainless Steel 120VAC 240V AC 50 60Hz Two 2A time delay 5mm x 20mm fuse Through the power cord must be properly earth grounded RS 232 straight through serial cable 15 to 40 C 59 to 104 F 0 to 85 no condensation 20 to 65 C 4 to 149 F Up to 2000m 40 80psi 1 SCFM 25 in Hg min 1 2 SCFM 27
11. late that 1s used on the calibration procedures a standard 96 shallow well SBS Plate is usually between 13 5mm 14 0mm Dwell Time This is the delay time the aspirate head stays in the plate after it stops at the defined depth location This setting is set in seconds default is 0 5 seconds Prime Volume This is the amount of fluid in uL the RapidWash primes when the unit switches it liquid path during a wash program 17 Initialize on Powerup This will allow the RapidWash to automatically home when the unit is turned on The default setting is auto initialize is set to on Set Dispense Pressure This option has been removed 12 10 2013 Calibration tab This screen allows you to recalibrate the positions saved in the RapidWash Figure 20 Dispenser Settings Figure 20 Prime Position This is the saved position of the dispense manifold when it primes the RapidWash Figure 21 18 Figure 21 Aspirate Origin This is the saved position of the aspirate manifold when the RapidWash begins to aspirate This position is calibrated with a 96 well teaching plate The position should be taught where each set of tips are centered in each well of the plate Figure 22 Figure 22 19 Dispense Origin This is the saved position of the dispense manifold when the RapidWash begins to dispense This position is calibrated with a 96 well teaching plate The position should be taught where each set of tips are centered in ea
12. ure source 9 from figure 1 from the rear of the RapidWash to top of bottle 1 4 blue tubing See Figure 2 e Level Sensor positioned near bottom of bottle See Figure 2 e Liquid Out From bottle to side port of RapidWash Select Manifold See Figure 4 e Vacuum In From vacuum source to top of bottle See Figure 3 e Liquid In From side port of RapidWash Select Manifold to top of bottle See Figure 4 e Level Sensor positioned near top of bottle See Figure 3 Note The Level Sensors for source liquids and waste are identical Only their positioning on the bottles must be different as described above 1 2 Rapid Wash Select Manifold All Liquid tubing is attached to the Select manifold on the side left side of the RapidWash Figure 4 All the ports on the Select Manifold are color coded so the matching color fitting should be plugged into the appropriate port The Color Coding is as followed Blue Liquid 1 Bottle Green Liquid 2 Bottle Yellow Dispense Head White Aspirate Head Black Priming Trough Waste Red Waste Bottle When all the tubing is installed on the RapidWash it should look like the Layout in Figure 5 RapidWash Select Manifold DISPENSE ASPIRATE PRIMING LIQUID 1 sete 2 HEAD HEAD TROUGH WASTE Figure 4 PRIMING TROUGH SSD t TARRAA t NJA fa VE ie O Figure 5 Section 2 Operation of RapidWash through the Touch Screen Controller 2 1 Key P
13. y ronmental aisun ea a iaa 27 Section 1 Setup 1 1 Tubing and Connections 1 J 3 4 Figure 1 Touch Screen Display Connection RS 232 Communication Connection AC Inlet For Power Cable Liquid 1 Level Sensor Position around Liquid 1 bottle close to bottom See Figure 2 Liquid 2 Level Sensor Position around Liquid 2 bottle close to bottom See Figure 2 Waste Level Sensor Position around Waste bottle close to top See Figure 3 Vac Control Output for remote control for external vacuum Air In From air source 40 70 PSI to rear of Rapid Wash this for the internal pressure regulator and optional plate gripper Pressure source for Liquid 1 and 2 1 4 OD tube connected between Liquid 1 liquid 2 bottles and rear of RapidWash See Picture 2 Liquid and 2 Dispense Bottle Tubing for HI Liquid 1 or 2 Tubing to Liquid 1 or Liquid 2 Port on Select Manifold Pressure source i Liquid Level H Sensor location Figure 2 Waste Bottle Waste Level Sensor location Tubing For Waste Port on Select Manifold Tubing to Vacuum Source Figure 3 1 2 Bottles Liquid 1 e Pressure In From 12psi pressure source 9 from figure 1 from the rear of the RapidWash to top of bottle 1 4 blue tubing See Figure 2 e Level Sensor positioned near bottom of bottle See Figure 2 e Liquid Out From bottle to side port of RapidWash Select Manifold See Figure 4 Liquid 2 e Pressure In From 12psi press
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