Micro-Incubator Model CSMI User`s Manual
Contents
1. Micro Incubator Harvard Apparatus Model CSMI Introduction The CSMI is a versatile Microscope Stage Incubator for cell tissue culture work which accommodates new rectangular disposable Chambered slides and Chambered cover glass available from Nalge Nunc and Beckton Dickinson The CSMI in combination with a matching low noise TC202A temperature con troller facilitates long term maintenance of tissue or cell cultures on a microscope stage This allows extended time optical monitoring including the Nalge du use of dyes microinjection electrical recording Chambered Slide and micromanipulation The CSMI not only facilitates the precise regula tion and manipulation of bath temperature but also supports multi channel perfusion and gas atmosphere maintenance such as CO2 superfu sion for pH control Based on a successful Medical Systems Micro incubator design for 35 mm Petrie dishes the PDMI 2 the CSMI utilizes Peltier Thermo Electric Devices to regulate tem perature over a wide range both above and below Nalge Nunc ambient levels The CSMI is designed to fit on the Cini stage of inverted microscopes from nearly all major microscope manufacturers including Zeiss Nikon Olympus and Leica Nalge Nunc Chambered Slide CSMI with Nalge Nunc Chambered Slide Nalge Nunc Nalge Nunc Chambered Coverglass amp Beckton Chambered Slide Dickinson Chambered Slice 4 Micro Incubator Harvard Apparatus Model CSMI2. Principle of Operations The CSMI is composed of two metal plates a finned Radiator plate and a Driven plate between which are sandwiched Thermo Electric Devices TED s or Peltier heat pumps The disposable chambered slide or chambered cover glass is placed by the user within temperature regulated clamps attached to the Driven plate The TED s pump heat from the radiator to the Driven plate for heating and from the Driven plate to the radiator for cooling The direction of the heat pumping action is determined by the direction of DC electric current delivered to the TED s Through the use of a temperature sensor mounted on the Driven plate and an external temperature controller TC202A that uses feedback control precise temperature regulation can be achieved both above and below ambient tem perature If perfusion is required two 26 GA Teflon perfusion lines can be mounted in each of the L shaped temperature driven claps A separate aspirator is used to maintain fluid level irrespective of perfusate flow rate Gas for superfusion over the chamber is delivered to a white 1 16th in barb port also attached to each L clamp The exit for the gas is a narrow opening on the inner long surface of each L clamp facing the installed chamber Having the perfusion lines and superfusion gas fed through the temperature regulated L clamps assures that fluid and gas delivered to the chamber are pre temperature regulated before they ar
3. maintained The Drawing below depicts the auxiliary radiator in place Micro Incubator Harvard Apparatus Model CSMI Maintenance Perfusion tubing replacement The installed Teflon capillary perfusion tubing can be used many times if kept clean The perfusion lines should be flushed with distilled water after use to avoid cross contamination Occasional perfusion of 7096 alcohol will help avoid growth of microorganisms Should replacement of perfusion tubing be required a spare set of two lines is included with each CSMI The perfusion tubing is pre formed to the circuitous shape of the grooves into which it is to be installed to makes it eas ier to install the rather stiff Teflon capillary To o E of Perfusion tubing obtain additional sets order PET MD e ricos To replace the perfusion lines follow the steps 1 6 referring to the drawing of the temperature driven L clamp below 1 Remove holding the chrome plated L clamp cover 2 Remove the Chrome plated cover and the black tubing cover resting inside to reveal the perfusion lines 3 Note the way the perfusion lines are installed in their heat transfer slots The two lines are not the same but are shaped differently to fit in different positions in the L clamp 4 Lift and discard the installed tubing and replace with a new set 5 Make certain that the tubing rests fully in the heat transfer groves and install the black tubing cover 6 Replace the
4. 1 8 Tygon hose is supplied for this purpose Both L clamps have gas ports These can be used simul taneously or separately for the same or different gasses The drawing below depicts the hookup for gas superfusion Operating the temperature controller with gas superfusion is similar to operation in static or perfused mode Because of feedback control the temperature controller will automatically adjust power delivered to the heat pumps to compensate for the additional load imposed by gas superfusion irrespective of whether plate or bath control is used Gas superfusion can be done in conjunction with fluid perfusion as well as with static protocols 7 Special considerations for cooling At room temperature the CSMI is more efficient in heating than cool id ing the disposable chambered slide miu G Although the CSMI can easily attain o and hold any temperature between ambient and 50 C it is limited to EE cooling to a range of 5 to 10 C ye below ambient depending on avi TITITIT iH mounting particulars This is due to the limited efficiency in heat removal afforded by the air radiating fins combined with the microscope stage For users who want to cool to lower tem peratures than this an optional auxiliary copper water radiator tube is available that fits in a slot cut into the radiating fins Running tap water in this auxiliary radia tor improves cooling performance greatly so that single digit temperatures can be
5. Chrome cover and re fasten with the two Phillips screws and 1 knurled knob removed earlier Perfusion tubing installation drawing Perfusion tubing installation drawing Micro Incubator Harvard Apparatus Model CSMI Maintenance Replacement of elastic Thermo conductive L clamp lining Each L clamp is lined with flexible Thermo conductive material on all inner walls that come in contact with the disposable chamber The lining is designed to con form to irregularities in the surface of the disposable chamber as the L clamps close Tight air pocket free contact between the L clamps and the chamber is required to guarantee maximum heat transfer between the temperature driven L clamps and the disposable chamber The lining can be used for many open and close cycles Should it become damaged it can easily be replace by the user A spare set of self adhesive lining pads is packed with each system To reorder use Cat 7501 125 To replace 1 Open the L Clamps to their full open position and remove any chamber within 2 Peel the existing lining pads off from the inner chamber wall 3 Clean the surface that held the pads with alcohol use Q tip or equiv and let dry 4 Remove the backing from the adhesive surface of a new lining pad and attach to the long surface of an L clamp Position carefully so that the Gas inlet slit near the top of the L clamp is not blocked 5 Repeat the procedure with a short lining
6. nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDEN TAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MER CHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as po
7. Chambered cover slips which are shorter than Chambered slides Once the disposable chamber rests on the floor of the CSMI slide the L shaped clamps inward diagonally to the closed position with care so that the contents are not spilled Make sure the clamps are firmly pressed against the sidewalls of the disposable chambered slide or cover slip This is to insure good thermal contact between the clamps and the chamber wall for proper heat transfer and temperature control The inner chamber contacting surfaces of the clamps are equipped with a Thermo con ductive elastic lining This lining is designed to mold itself around imperfections and high spots in the injection molded chamber walls to prevent air gaps that might reduce heat transfer and performance L Clamps slide at an angle to open and close Micro Incubator Harvard Apparatus Model CSMI Hookup and Operation 1 Connection to Temperature Controller Please also refer to the TC202A Users manual for additional operating instruction To prepare for operation the CSMI is connected to a TC202A temperature con troller by plugging its cable end plug into the I O socket on the TC202A front panel The TC202A can sense CSMI Driven plate temperature through this cable as well as supply power to the Peltier heat pumps The TC202A also supports an optional second temperature sensor the Bath Thermistor which can be insert ed in the disposable chamber media to me
8. Micro Incubator Model CSMI User s Manual Model CSMI Micro Incubator MAI 65 0101 MA1 65 0102 HARVARD APPARATUS Publication 5403 002 Revision A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products
9. and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required z Harvard Apparatus Model CSMI Micro Incubator Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 Specifications iii ai 3 INTOQUCUON siria 4 Principle Of Operation eue 5 Supplied and Optional Accessories 6 7 Mounting the CSMI on a Microscope Stage 8 10 Installation Disposable Chambers in CSMI 11 Hookup and Operation 12 15 Maintenance eere rere een 16 17 IFOUDIe SHOOllg casi ini 18 Appendix 1 Typical Performance Curve 19 Micro Incubator Harvard Apparatus Model CSMI General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse
10. asure temperature therein The Bath Thermistor is not always used if it is it has its own cable and plug and should be connected to the TC202A at this time Drawing or photo of CSMI connection to TC202 2 Using the Plate or Bath Thermistor for temperature feedback and control During operation there generally is a difference in temperature between the tem perature Driven Plate and the media in the disposable chambered slide Bath The gradient between Plate and the center of the Bath could be 1 to 4 C depend ing on such factors as thermal resistance of the chamber wall media depth per fused or static operation gas superfusion etc The offset is positive or negative depending on whether one is heating or cooling Though we always want to control temperature in the bath often Plate control is the best solution This is the case for example in situation where media sterility is required and the burden of sterilizing the bath thermistor makes it impractical The Plate temperature can be offset manually to stabilize at a higher or lower tempera ture in order to compensate for any difference between bath and plate Micro Incubator Harvard Apparatus Model CSMI Hookup and Operation For the most part the safest mode of operation is to use the Plate thermistor as the control point Plate control has the advantage of not being subject to unintended 4 open loop operation where temperature control is lost This
11. can occur acci dentally with bath control but is highly unlikely with plate control A bath ther mistor can become accidentally dislodged from the bath so that it no longer meas ures bath temperature During extended operation media evaporation can uncover the bath thermistor sensing tip causing it to read incorrectly In this case the tem perature controller loses control With plate control bath temperature can still be precisely monitored by a Bath thermistor but control will not be dependent on it Plate control also has the advantage of bringing the system to the control point temperature faster than Bath control The bath temperature lags the plate temper ature This lag causes the system to take longer for bath temperature to settle at the control set point With the above caveats in mind bath control has a major advantage over plate con trol With the bath thermistor as the control point the temperature controller automatically compensates for the temperature gradient between plate and bath and for changing environmental conditions including perfusion changes Probably the best strategy is to quickly bring the bath to the target temperature using plate control Then switch to bath control for the greatest precision in long term temperature maintenance 3 Installation of optional Bath Thermistor The Bath Thermistor is inserted in the media and held in place using the supplied magnetic base thermistor holder which atta
12. ches to the ferrous chrome plated top surface of each L shaped clamp If the plastic cover with magnetic frame is to be used over the chamber in conjunction with the Bath thermistor it can either be slid open partially to accommodate the thermistor Alternatively a 1 8 hole can be drilled in the cover where the bath thermistor is to pass through 4 Static operation For static or non perfused operation a chamber with media is installed and a set point is entered into the temperature controller If 37 C is selected it will take approximately 25 minutes for the temperature to stabilize starting from room tem perature At this higher temperature long term incubation will result in media loss through evaporation Using mineral oil over aquious media can slow this If min eral oil cannot be used the clear plastic cover which is supplied can also reduce evaporation Micro Incubator Harvard Apparatus Model CSMI Hookup and Operation 5 Perfused Operation Perfusion is a useful tool to study pharmaceutical effects both in applied and washout modes lo establish perfusion perfusate is delivered to the chamber through 26GA Teflon capillary tubing Two such perfusion lines are installed in close fitting slots in the temperature controlled L clamps that surround the cham ber The perfusate supply which can be kept at room temperature is warmed or cooled by conduction before delivery into the chamber bath The perfusate shoul
13. d be delivered at a rate of 1 to 3 ml min This is best accomplished by a peristaltic pump like the Harvard Apparatus model 66 catalog 55 7766 A set of two 1 8 Tygon hoses with couplers for the 26 GA Teflon capillaries are supplied to make the connection to the peristaltic pump or other perfusion source If perfusion is to be continuous than bath media must be removed to prevent over flow A unique adjustable aspirator the LU ASP catalog 4 65 0047 is supplied which can automatically maintain a constant fluid level with minimal fluctuations in that level as it works see adjacent drawing Fluid level fluctuations not only can disturb optical measurements but can also be a source of electrical noise if sensi tive Electro physiologic recording such as patch clamping is done The aspirator has a magnetic base to grip the magnetic surface on each of the L clamps The level of fluid in the chamber is determined by the height of the aspirator in the bath and can be adjusted with its thumbscrew A source of suction with a liquid trap is con nected via the supplied 1 16 Tygon tubing Because of the design of the aspira tor the user can operate one or more perfusion lines simultaneously without fur ther adjustment to the aspirator During perfused operation media is replenished continu ously and evaporation is not a problem until perfusion is halted If perfusion is to be interrupted the plastic cover should be used to minimize
14. evaporation The plastic cover is supplied without any holes drilled If it is to be used simultaneously with a LU ASP aspirator as is the case with a bath thermistor the user can partially slide the cover back or alternatively drill a hole to accommodate the aspirator The drawing below depicts the hookup for perfusion Operating the temperature controller for perfusion is similar to operation in static mode Because of feedback control the temperature controller will automat ically adjust power delivered to the heat pumps to compensate for the additional load imposed by perfusion irrespective of whether plate or bath control is used E Micro Incubator Harvard Apparatus Model CSMI Hookup and Operation 6 Gas superfusion Gas superfusion is often used to aid in pH balance 596 C02 as well as for improved oxygenation The CSMI has provisions for delivering gas via a cavity in the temperature regulated L clamps to a slit just over the edge of the disposable cham bered slide This causes the gasses to be delivered to the chamber pre tem perature regulated Heavier than air gasses like C02 will then fall into the chamber cavity With the aid of the supplied plastic cover a low pressure atmos phere of any desired gas mixture can be maintained in the chamber The gas is delivered from a tank equipped with a regulator and flowmeter to the white barb fitting at the long end of the temperature regulated L clamp A
15. pad Micro Incubator Harvard Apparatus Model CSMI Troubleshooting The following table lists some problems possible causes and remedies This is not meant to be exhaustive but as a checklist to allow the user to solve the more com mon occurrences Many problems arise from improper use of the TC202A tem perature controller Please refer to the TC202A user s manual if the problem you experience is not listed here Problem Description 1 The bath temperature does not reach a heating set point ta 1b 1c The L Clamps are not closed tightly around the chamber Check and fix Static fluid level is too low raise Perfusion rate is too high lower below 3 ml min 2 The bath temperature does not reach a cooling set point 2a 2b 2C 2d The L Clamps are not closed tightly around the chamber Check and fix Static fluid level is too low raise Perfusion rate is too high lower below 3 ml min Set point is outside of working range without auxiliary water radiator change setting or add auxiliary water radiator 3 Bath temperature is rising above ambient even though set point is for cooling below ambient 3a Set point is outside of temperature working range without auxiliary water radiator change setting or add auxiliary water radiator Excessive 50 60 Hz noise in Electro physiologic recording done from tissue in the chamber Bath temperature inaccura
16. pads to line the inner contacting surface of the L shaped clamps 7501 126 Clear plastic cover with magnetic hold down frame 7501 127 A set of 4 nylon set screws for use when mounting on Nikon or Olympus inverted microscopes Optional Accessories 65 0103 A copper water tube shaped to fit the slots in the CSMI radiator fins is available for applications where bath termperatures apporaching 0 C are required In this case running tap water in the installed copper tube is used to improve the efficiency of heat removal from the CSMI in order to achieve this lower temperature range 7501 127 Replacement tubing and Pad Kit containing all tubing and thermo Micro Incubator Harvard Apparatus Model CSMI Mounting the CSMI on a Microscope Stage The CSMI is equipped for easy mounting on microscope stages of several of the most popular inverted microscopes There are two major considerations when installing the CSMI first is mechanical attachment to the stage and second is heat transfer between the CSMI and the microscope stage Mechanical fixation is desir able because X Y translation of the specimen can then be done using the micro scope built in stage movement mechanism In addition when the CSMI is mount ed on a microscope stage the stage which is generally made of material like alu minum serves as a low Thermo resistance pathway to conduct heat to or away from the CSMI In general when
17. rews inserted into four tapped holes located on an 80 mm diameter in the bottom of the CSMI The setscrews protrude into the edges of this opening locking the CSMI to the stage for X Y translation Other For microscopes not mentioned above contact Harvard Apparatus Inc technical support to discuss mounting options Please Note Though CSMI can be rested and operated on a microscope stage surface Care must be taken that only the finned Radiator plate comes in contact with the stage The temperature controlled Driven plate must not touch any part of the microscope Micro Incubator Harvard Apparatus Model CSMI Installation of Disposable Chambers in CSMI The CSMI is a finned rectangular assembly with two temperature controlled L shaped clamps inside To install a disposable chambered slide or chambered cover slip refer to the drawing below First open the two L shaped clamps fully by sliding them diagonally away from the center Push on the knurled knobs at the corner of each clamp Caution do not try to force the clamps in the X or Y direction they only slide diagonally Once the Clamps are open carefully insert the Chambered slide or chambered cover slip so that it rests on the metal strips in the opening Note care must be taken that disposable chambers do not fall through the opening in the floor of the CSMI during installation spilling their contents This may be a problem on instal lation more so with
18. rive in the tissue culture dish Chamber Installed L clamp cover Gas entry port Perfusion tubing cover L clamp closed without perfusion tubing Diagram of CSMI parts Micro Incubator Harvard Apparatus Model CSMI Supplied and Optional Accessories The CSMI chamber depicted above is a flexible system that can be used in several modes of operation These include perfused and static modes with or without gas superfusion with a bath thermistor or without and of course mounted on differ ent microscope stages l he CSMI is shipped with the most commonly used acces sories which are packed separately and identified below Supplied Accessories Catalog No Description 65 0047 Leiden Aspirator on magnetic base LU ASP for media removal during perfusion 65 0055 Bath Thermistor holder on magnetic base 7501 121 7501 122 A set of two 4 lengths of 1 8 ID Tygon tubing for gas superfusion A set of two 4 lengths of 1 8 ID Tygon tubing with compler for 26 GA Teflon perfusion tubing for perfusion 7501 123 A 4 length of 1 16 Tygon tubing for use with the Leiden Aspirator Micro Incubator Harvard Apparatus Model CSMI Supplied and Optional Accessories 7501 124 One spare set of two 26 GA Teflon perfusion lines molded into proper shape for easy insertion in temperature driven clamps 7501 125 Two set of spare Thermo conductive self adhesive elastomer
19. ssible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION CAUTION This pump is not registered with NOT FOR CLINICAL USE the FDA and is not for clinical use on human patients ON HUMAN PATIENTS 4 Micro Incubator Harvard Apparatus Model CSMI Specifications Specifications Model CSMI Micro Incubator Temperature Operating Range With Supplementary Water Cooling Without Supplementary Water Cooling Disposable Chambers Accommodated Microscopes Accommodated Peltier TED Current Rating Plate Thermistor Built in Perfusion Inlet Lines Perfusion Rate Perfusate Outlet Gas Port Gas Superfusion Rate Weight Overall Dimensions CSMI Cable Plug Pin Assignment 5 C to 50 C 5 to 10 below ambient to 50 C Nalge Nunc chambered slides chambered cover slips Bekton Dikinson chambered slides Zeiss Axiovert with attachable mechanical stage Leica DAS Mikroskop DMIL and DMIRB E w attach mech stage Nikon Diaphot TE200 TE300 series rectangle stage Olympus X50 70 or IMT s fixed stage 6 A DC Maximum 100 k at 25 C YSI 44011 26 GA Teflon capillary up to 4 lines can be installed simultaneously 3 ml minute nominal total LU ASP aspirator 1 16 barb 0 5 to 2 0 L min 0 5 kg 17 9 oz 6 5 x 4 x 1 1 Peltier 2 Peltier 3 Plate Thermistor 4 Peltier 5 Peltier 6 Plate Thermistor Ground Shield
20. stage with the dimensions of 160mm x 110mm Both CSMI Cat 65 0101 and the 65 0102 can be used on microscope stages with FLAT surface and with a round opening between 88 mm to 110 mm To avoid vibration if any modeling clay or dentist wax could be applied at the edge of the micro incubator Microscope stage M in Mounting frame M for specimen Type 65 0101 tc dee 7 slider 76 x 26 mm Object guide m 130 x 85 mm right Object guide By 130 x 85 mm left gt Specimen stage 250 x 230 mm e 66 55 toe pr IAE lt gt Mounting frame K for specimen slider ype v2 SS Mounting frame K for reflected light d 72 mm Stage stop d 28 mm Stage stop d 35 mm Stage stop d 18 mm further stage stops being prepared Mechanical stage 130 x 85 R L without mounting frames Gliding stage Z T with stage inserts d 24 mm and 48 mm Micro Incubator Harvard Apparatus Model CSMI Mounting the CSMI on a Microscope Stage Nikon Nikon inverted microscopes have a stage opening that is 108 mm in diameter The CSMI is mounted over this opening using four nylon set screws inserted into four tapped holes located on an 108 mm diameter in the bottom of the CSMI The setscrews protrude into the edges of this opening locking the CSMI to the stage for X Y translation Olympus Olympus inverted microscopes have a stage opening that is 80 mm in diameter The CSMI is mounted over this opening using four nylon set sc
21. te or unchanging 4a Ab 5a CSMI ground not connected through TC202A mains protective ground pin in power cord or connected to poor ground Plug TC202A into better grounded power outlet or into same outlet as recording equipment Remove bath thermistor if used and use only plate thermistor and plate control Replace bath thermistor Micro Incubator Harvard Apparatus Model CSMI Appendix 1 Typical Performance Curve The above performance curve was obtained using a Nunc chambered coverglass chamber The bath chamber contained 2 5 ml of water under static conditions with a plastic cover slid back to accommodate the bath thermistor The dark blue curve represents the set point entered into the TC202A temperature controller The light blue curve is the temperature measured at the radiator fins o Nuptiis d pebRetRPOQQoPbPptobeecd heo Time minates
22. the CSMI is placed on a flat microscope stage it is the Radiator portion that comes in contact with the stage The Driven temper ature regulated plate that holds the specimen is recessed slightly and does not come in contact with a flat stage Specimen cooling performance of the CSMI is improved by extra heat removal through the microscope stage Specimen heating performance is less affected by mounting considerations The following table describes how to mount the CSMI on various brands of Inverted Microscope Brand Description Zeiss The CSMI is designed to fit directly into the holding clips of the Attachable Mechanical Stage available with Ziess inverted microscopes with the M type Mechanical Stage For K type 3 Plates Stage CSMI will need to be modified Call Harvard Apparatus Leica Inverted microscopes The CSMI is designed to fit directly into the holding clips of the Attachable Mechanical Stage available with Leica inverted microscopes s Micro Incubator Harvard Apparatus Model CSMI Mounting the CSMI on a Microscope Stage Note The micro incubator CSMI Cat 65 0102 has the same construction as the regular CSMI Cat 65 0101 with the difference that the bottom plate of the 65 0102 has been designed to be used in the following microscopes ZEISS and LEITZ with 3 Plate mechanical Stage known as K type LUDL and PRIOR motorized stage in inverted microscopes The 65 0102 will fit on the microscope
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