NeuroWare® User`s Manual
Contents
1. Stim 2 Ware WIRELESS RF Stimulation User s Manual a division of Harvard Bioscience Inc Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 e Fax 919 544 3061 www trianglebiosystems com STIMWARE User s Manual Table of Contents Document CVS VN ace sens wets E cae acne wn ee E E E E en 2 1 gil 8 54 81 m E Geen en ne ne eo re ee ee eee 2 Headstage PINOUT sssrin e EEEE E E n 3 MNE 2 OIG CE OS carefree cass E connused vere Seatac S E es nieuiaewed Connenane 3 Hardware UNS Cea CO coc a E er sree naan cee die teased apc snaeeceetacd ee aceedseeesaaneecananene 4 SOP Oe INSTA AU oN E quer aneceoseecaesena te anesen aes 5 System Requirements si ssdicisicsetieercs cede cacecatGciessadasincecathowsscatauesesathewranslavecessahseracebeecesstbewminietetesetserss 5 St mWare US aN NN rege ee ress carter E R 5 Sotware NAV IS AU OM ice ccs sect raceneecostaneoncieasncacesslosmubaiocentesnueeeedhsierseacemnioouaiaovealserbaroaean sess sanctosdsuebaincsnneseseanienseatees 7 LOACING SAVING CONMCULATIONS cosisaasicesricanicesadsativedunsaawsanionssacanrissaadopiivedsepnsusesicssiiaaneysacniepeiwadseenseeanaaeitaes 7 Programming the Stim Channels scccsetnccuscicieesdacccsayieesecexscetbeareesscewdencessenmnecessxneceeeriendevanweedenstueeemeeeeseedenenes 8 Ve oi MU elec ge To a deren eae ec me en ee ee ee eee 9 PTD ACL ON NO SE een noes ce Grstnnegs E2. Train Duration TD EA 2o Download 2 ippi Stimulus iple Trai Clipping pc E Multiple rains Zero Stimulus Frequency SF m afos Hz a Stimulus Duration SD 5 200 s j 9 22 2015 10 29 AM Trigger Command Ack 9 22 2015 10 29 AM Trigger Command Downloaded 9 22 2015 10 28 AM Pattern Ack 9 22 2015 10 28 AM Pattern Downloaded 9 22 2015 10 28 AM Select HS ACK 9 22 2015 10 28 AM Select HS 2 Downloaded a Dongle Headstage Pattern La La Lal om Figure 68 Triggering Pattern 4 2 External Trigger Two external triggers are supported As Figure1 shows one end of trigger cable can be plugged in dongle and the other end are two bare wires that can be connected with external system in which external trigger signals are generated Red wire is external trigger signal whose voltage is up to 5V Black wire is ground and should be connected with the ground of external system All external trigger settings are located in Set up tab of StimWare as Figure16 shows Either rising edge or falling edge can be selected as active trigger edge The channel should be disabled to avoid false trigger when you finish experiment or when you don t use external trigger Trigger setting won t take effective until you click Set Up External Trigger button Version 1 6 13 4 10 2015 STIMWARE User s Manual Channel1 7 Channel2 7 Search HS Setup
3. Turn On HS Turn Off HS Download Tri Pattern ngger Clipping i l DC Offset Reset Clip ill 9 22 2015 9 55 AM 2 HS ACK 9 22 2015 9 54 AM Search HS 2 Downloaded 9 22 2015 9 54 AM Dongle Detected Dongle Headstage Pattern i 2a Figure 13 Searching for Headstage Channell v l Channel2 v Search HS Setup Dongle Version Ss reg al a oe tery pears oar vi 23 0 Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS en pa Stepd Select HS in list with mouse G 2 J 2 and click on Select HS Step5 Download pattern to selected HS or send cmd to it C 2Ch STIMWARE V1 7 0 4 Default Config File ini Turn On HS Turn Off HS Download Tri Pattern ngga DC Offset Zero lll Dongle Headstage Pattern wl 2 Available Headstages 9 22 2015 9 58 AM Select HS ACK 9 22 2015 9 58 AM Select HS 2 Downloaded 9 22 2015 9 57 AM 2 HSACK 9 22 2015 9 57 AM Search HS 2 Downloaded 9 22 2015 9 57 AM Dongle Detected Figure 54 S electing Headstage Version 1 6 10 4 10 2015 STIMWARE User s Manual Multiple Headstage If you have multiple headstages with different address you will be able to control each headstage with StimWare At one time dongle can only communicate with one headstage Once the headstages are charged Set up Start Address a
4. lt Channell v Channel 2 Search HS Setup Stim GE Ware Dongle Version See Ae vi 230 Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS Step4 Select HS in list with mouse and click on Select HS Step5 Download pattern to selected HS or send cmd to it C 2Ch STIMWARE V1 7 0 4 Default Config File ini Turn On HS Turn Off HS Download Tri Pattern ngger Clipping E DC Offset Zero Reset Clip Dongle Headstage Pattern 9 22 2015 10 18 AM DC Offset Adjust Done A a 9 22 2015 10 18 AM Zero Command Ack 9 22 2015 10 18 AM Zero Command Downloaded 9 22 2015 10 15 AM Select HS ACK Quit Stimware 9 22 2015 10 15 AM Select HS 2 Downloaded 9 22 2015 10 15 AM 2 HS ACK APIA NnAraAInar Ak Cas te aN Figure 16 Removing DC Offset 4 Generate and download pattern in StimWare Choose the stim signal parameters you wish to upload to the headstage Refer to the Software Navigation section of this document for more pattern programming instructions When finished click the Download Pattern button to send the pattern data to the headstage The pattern will be saved on headstage until you download new pattern Channell y Channel2 v Search HS Setup Stim Ware navaa Once following trigger Pulse Current PIL Pulse Duration PD1 DC Offset mV Inver PDI Heo m
5. Stim 6 Ware External Trigger Setting Set Up Chi Enable Chl Edge Selection External Trigger J Rising Edge x Ch2 Enable Ch2 Edge Selection F Rising Edge x C 2Ch STIMWARE V1 7 0 Default Config File ini Turn On HS Turn Off HS Download Tri Pattern ngges Reset Clip ll Dongle Headstage Pattern 9 22 2015 11 34 AM Set Trigger Ack a Set rigger Downloade 9 22 2015 10 29 AM Trigger Command Ack 9 22 2015 10 29 AM Trigger Command Downloaded Quit Stimware 9 22 2015 10 28 AM Pattern Ack 9 22 2015 10 28 AM Pattern Downloaded S API PIAA AAMA Ana Ii eter arw Figure 19 External Trigger Setting When valid external trigger is detected it will trigger the pattern loaded in headstage Trigger Cable will show up in log window and Pattern indicator starts blinking Channell v Channel2 v Search HS Setup Dongle Version eaea i aaa vi 23 0 Stepl Power on all HS for example 6 HS z Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS _ Step4 Select HS in list with mouse G 2 G 2 and click on Select HS Step5 Download pattern to selected HS or send cmd to it Stim Ware C 2Ch STIMWARE V1 7 0 4 Default Config File ini Turn On HS Turn Off HS Download Tri Pattern ngges Clipping lll DC Offset Zero Reset Clip lll 8722 2015 12 10 PM Trigger Cablel 9
6. T 200 jua gt us j PIZ CA2Ch STIMWAREVL7 0 4 Default Config File ini V Train Multiple Pulses Train Frequency TF Train Duration TD Download 120 s 7 i TE _ Stimulus Multiple Trains Clipping ill DC Offset 7 Zero Stimulus Frequency SF Reset Clip i E m 0 005 Hz _ _ _ __ __ _ kl Stimulus Duration SD 1200 s f l Dongle Headstage Pattern 9 22 2015 9 41 AM Dongle Detected Figure 12 Dongle Recognition Version 1 6 9 4 10 2015 STIMWARE User s Manual 2 Search and Select Headstage Once the headstage is charged Click the Search HS tab Press the Search HS button on that window and you will see your headstage under Available Headstages Once your HS is visible press the Select HS button and the green Headstage light at the bottom will illuminate and turn on Headstage Channel1 v l Channel2 v Search HS Setup l Stim Ware C 2Ch STIMWARE V1 7 0 a 4 Default Config File ini Dongle Version 1 23 0 Start Address End Address CC siiri Ey Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS Step4 Select HS in list with mouse and click on Select HS Step5 Download pattern to selected Available Headstages HS or send cmd to it T
7. 22 2015 12 10 PM Trigger Cablel 9 22 2015 12 10 PM Trigger Cablel Dongle Headstagef Pattern a La Co mW gt BO eo ELEV F IYD F gy SUVNI 9 22 2015 12 10 PM Trigger Cablel 9 22 2015 12 10 PM Trigger Cablel Arn rma r aN an mae Figure 20 External Triggering Version 1 6 14 4 10 2015 ec es eCG STIMWARE User s Manual TBSI e Rising edge active If Rising Edge is selected in Ch Edge Selection the rising edge of external trigger signal will trigger pattern If the positive pulse T1 is more than 4ms it s regarded as valid trigger The pattern will start after delay T2 If the positive pulse T1 is less than 4ms it s regarded as false trigger and ignored and the pattern won t start T3 is interval between the end of pattern and the next coming trigger edge Users should set up the reasonable pattern duration by considering T4 which is interval between the two consecutive trigger pulses I T1 l P VH i I l Trigger Signal VL i l i l Pattern Start Pattern Stop i l l i l i 5 i Rising edge i i gt i i I I M ae eee epee ena ese l l i l e l I l l 7 I T2 gt T3 ie l l l l l Figure 21 External Trigger Rising Edge Active e Falling edge active If Falling Edge is selected in Ch Edge Selection the falling edge of external trigger signal will trigger pattern If the negative pulse T1 is more than 4ms it s regarded as valid
8. E naa eeicienias acing snanet EO 18 BNC CROCS serie E tietudoneseateadetteanate tedcne ea cue dina eA tueee tee aachornedandadeedonaeaaens 18 AAT SOO IE arcsec er cb E erases antes bees pesca anaes 18 Version history for this Manual essssssssssssesssrerssrrerssrrressrersssrresrreesserressreressreesereresereessrerosereeserereseeeesseee 19 Version 1 6 1 4 10 2015 STIMWARE User s Manual TBSI Document Overview This document will demonstrate how to use the TBSI STIMWARE hardware and software The program controls a single 2 channel headstage that allows researchers to generate and upload two separately customizable waveform patterns via a wireless USB dongle The headstage is battery operated therefore only needs to be connected to the charging plug for recharging Shipped Items List e 2 Channel Stimulation Headstage e StimWare Installation CD e USB Dongle e Headstage Charger e 2 Trigger Interface Cables e Stim Signal Test Load Board USB Dongle Headstage Installation CD 2012 Triangle BioSystems international inc 2224 Page Road Suite 108 Durham NC 27703 919 361 2663 T 919 544 3061 F www trianglebiosystems com Headstage Charger Trigger Cables Load Board Figure 1 Images of Items Version 1 6 2 4 10 2015 Bottom Figure 2 Pin Mapping eee a SP Figure 3 Image of Pins on Headstage e Sullins M50 4900545 STIMWARE User s Ma
9. oC z _ Stimulus Multiple Trains Clipping i DC Offset z Zero Stimulus Frequency SF E SF TD 10 005 Hz n e Stimulus Duration SD Reset Clip Dongle Headstage Pattern ll ll lll Figure 10 Saving Parameters Programming the Stim Channels The two stim channels can be enabled and programmed separately from each other To enable a channel select the checkbox next to its name A channel that is unchecked will not output any signal so it may be advantageous to disable an unused channel to conserve battery life Channell v Channel2 v f Search HS Setup l Stim f Ware wOliin PL FFaQrw Initial Delay ID Once following trigger Pulse Current PIL Pulse Duration PD1 DC Offset mV Invert PD1 m ry jua ry go i k 4 yo us ew 19200 a50 us Pulse Current PI2 ie Duration PD2 ma ry altance 21200 uA j PIZ C 2Ch STIMWARE V1 7 0 A us lal Charge 4 Default Config File ini l V Train Multiple Pulses Train Frequency TF Turn On HS Turn Off HS 15000 Hz a Train Duration TD ie TEEI ES zN wnload 420 S aiad Trigger p TD _ Stimulus Multiple Trains lll DC Offset Zero Stimulus Frequency SF i Reset Resetcip_ p TD i 0 005 Hz a Stimulus Duration SD Dongle Headstage Pattern lll ll Version 1 6 8 4 10 2015 STIMWARE User s Manual Figure 11 Distinguishing Se
10. x lt j 200 uA 50 us x Afo E Pulse Current PI2 pa Duration PD2 es i Yi alance Se pew i C 2Ch STIMWARE V1 7 0 4 us lal Charge 4 Default Config File ini V Train Multiple Pulses Train Frequency TF Turn On HS Turn Off HS 5000 Hz Train Duration TD Download a A 20 S Clipping E _ Stimulus Multiple Trains Stimulus Frequency SF Reset Clip gean y SF TD Stimulus Duration SD 1200 S v j 9 22 2015 10 24 AM Pattern Ack 9 22 2015 10 24 AM Pattern Downloaded 9 22 2015 10 18 AM DC Offset Adjust Done 9 22 2015 10 18 AM Zero Command Ack 9 22 2015 10 18 AM Zero Command Downloaded 9 22 2015 10 15 AM _ Select HS ACK AANI anar a ad a ft gt Dongle Headstage Pattern cell ll m Figure 17 Downloading Pattern Version 1 6 12 4 10 2015 STIMWARE User s Manual 4 1 Software Trigger Press Trigger to start the pattern The Pattern light will illuminate and blink while the pattern is running and will stop blinking when pattern is over Channel y Channel2 V SearchHS Setup Stim Ware suos o Once following trigger Pulse Current PIL Pulse Duration PD1 DC Offset mV Invert A T E m A 200 fua afso us z Afo E Pulse Current PI2 Duration PD2 ma m a eel PD1 k PI2 C 2Ch STIMWARE V1 7 0 a 4 Default Config File ini E V Train Multiple Pulses Train Frequency TF
11. ment 7 KB E Recent Places J silabs cde 4 10 2014 3 50PM Security Catalog 9 KB 2 Dropbox 4 4 2014 11 50 AM Setup Information 3 KB I 10 24 2013 11 39 Application 1 026 KB Libraries Silabs CDCInstaller_x t 10 24 2013 11 39 Application 901 KB Documents al Music Pictures E Videos bh 6 items Figure 5 Select dongle driver Version 1 6 5 4 10 2015 TBS STIMWARE User s Manual 3 Plug the USB dongle into PC Silicon Labs CDC Serial port driver will be installed Silicon Labs CDC Serial port COM10 installed Silicon Labs CDC Serial port COM10 wf Ready to use Figure 6 Dongle driver installation done 4 Right click on My computer and select Manage to open the Device Manager If you do not have My computer on your desktop click the start button then right click on Computer and select Device Manage Dongle should be indicated by Silicon Labs CDC Serial port COM under COM and LTP a Y Ports COM amp LPT 3 s Communications Port COM A a Silicon Labs CDC Serial port COM10 Figure 7 Information in Device Manager Version 1 6 6 4 10 2015 A STIMWARE User s Manual TBSI_ Software Navigation Launch the StimWare software application StimWare is in Run mode once launched 9 Figure 8 StimWare Launch Screen Loading Saving Configurations Default parameters are loaded into StimWa
12. nd End Address based on headstage s address range Click the Search HS tab Press the Search HS button on that window and you will see your headstage listed in Available Headstages table Once your headstages are visible click on headstage that you want to control with mouse and press the Select HS button The green Headstage indicator at the bottom will illuminate and selected headstage address will be shown right inside indicator ee Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS Step4 Select HS in list with mouse and click on Select HS Step5 Download pattern to selected HS or send cmd to it C 2Ch STIMWARE V1 7 0 4 Default Config File ini 9 22 2015 10 10 AM Select HS ACK 9 22 2015 10 10 AM Select HS 3 Downloaded 9 22 2015 10 09 AM 3 HSACK 9 22 2015 10 09 AM Search HS 3 Downloaded 9 22 2015 10 09 AM 2 HSACK 9 22 2015 10 09 AM Search HS 2 Downloaded Dongle Headstage Pattern ll l MI gt Figure 15 Multiple Headstage Detection 3 Remove possible DC Offsets Click the DC Offset Zero button to set the signal level to OV before downloading a pattern The light will blink yellow while the DC offsets are being removed DC offset adjust should only be performed when resistance changes Version 1 6 11 4 10 2015 STIMWARE User s Manual
13. nual Hardware Installation Computer StimWare Software A n j ma Headstage castle USB Dongle Sullins Connector N Trigger 1 Trigger 2 External Stimulation Triggers TTL Pulse Figure 4 System Setup Diagram Version 1 6 4 4 10 2015 STIMWARE User s Manual Software Installation System Requirements The recommended minimum PC requirements are as follows e Atleast 1 0 GHz Processor e 2GBof storage memory e 1GBRAM e 1 USB 2 0 port e Windows 7 StimWare Installation To install and run StimWare 1 Insert the StimWare installation disc into your computer Navigate to STIMWARE Installer folder within the installation disc and double click setup exe then follow the prompts until installation is complete 2 Navigate to Silabs CDC_ Install folder within the installation disc and double click Silabs CDClinstaller_x64 exe if Operation System is 64bit or Silabs CDClInstaller_x86 exe if Operation System is 32bit then follow the prompts until installation is complete Search Siiabs CDC Install D GOGO Ji EFM32 USB driver Silabs CDC_Install S Organize Include in library Share with Burn New folder es sir Favorites Name j Date modified Type W Desktop dpinst 3 25 2014 3 05 PM AML Document 11 KB mi Downloads q Silabs_License_Agreement 6 16 2013 11 55 AM Text Docu
14. parate Channels The graphics in the StimWare interface are intended as conceptual references and do not reflect the actual pattern set by the user defined parameters The Initial Delay ID will be executed once at the start of the pattern By clicking the Balance Charge button the software will verify and or balance the current of the individual pulses Checking the Invert box will change the polarity of the entire signal To enable higher order tiers check the box next to its name Keep unused tiers disabled if they are not in use Certain parametric limitations are programmed into the software to ensure signal consistency and prevent saturation of the headstage amplifiers If a parameter has exceeded its allowed range ex a frequency is set too high in a higher order tier it will automatically be reset to its highest allowed value PI1 and PI2 can t exceed 500uA PD1 and PD2 can t be lower than 50uS System Setup and Testing 1 Connect USB Dongle to computer Open the StimWare program application If the dongle is successfully recognized by your computer the Dongle indicator will illuminate green in StimWare Channel 1 Channel2 v SearchHS Setup l Stim f Ware WOilil PL WFQIW Initial Delay ID Once following trigger Pulse Current PIL Pulse Duration PD1 DC Offset MV invert PDA y y y i 0 E k 160 us x A200 uA SE J open saves Pulse Current P2 Pulse Duration PD2 Banc F i nce
15. re from the configuration ini file located on your computer gt C 2Ch STIMWARE IF Enter Configuration Filename JCJ N gt Computer OS C 2Ch STIMWARE VL7 0 s Search 2Ch STIMWAREV17 0 Name Date modified Type Size Libraries oe 2CH Stimware Setting 9 14 2015 2 05PM Configuration sett 1KB r r 2CH STIMWARE V1 7 0 9 14 2015 2 10PM Configuration sett 1KB at Default Config File 9 14 2015 2 04 PM _ Configuration sett 1 KB lee Pictures EE Videos Jii Computer Boo ca Work E Gi TBSL folder TB ia Network bd File name Default Config File 7 Figure 9 Loading Configuration File Version 1 6 7 4 10 2015 STIMWARE User s Manual Customized pattern parameters can be saved by pressing the Save As button The new configuration file by default will be saved to C 2Ch STIMWARE Channell y Channel2 v Search HS Setup Stim BE Ware naoso Once following trigger Pulse Current PIL Pulse Duration PD1 DC Offset mV Invert E y ry ry ry 160 us J 200 uA p50 us M fo E sen Pulse Current P2 Pulse Duration PD2 gt A F alance lt p 200 uA 50 us d Piz C 2Ch STIMWAREVL7 0 A J A Charge 4 Default Config File ini 5 V Train Multiple Pulses i 1 Train Frequency TF TF Turn On HS Turn Off HS 5000 Hz oer Train Duration TD a B S ae ee A ES oad 5 emt tape on
16. s Inc NeuroNexus Neuralynx and CD Neural Technologies offer a variety of electrode arrays that connect to all of our headstages Troubleshooting Problem The headstage won t turn on Version 1 6 18 4 10 2015 STIMWARE User s Manual iste Suggestion Charge the battery via the charging plug provided with the system Refer to the section titled Application Notes for charging instructions Problem One or more output signals are missing or incorrect Suggestion Download the stim signal parameters to the headstage with StimWare making sure to follow all instructions within this manual Check to make sure that the channels are enabled within the software prior to downloading Check all equipment used to monitor the signal to ensure continuity Call TBSI customer support at 919 361 2663 if there are any problems with system setup and function You can also submit a Technical Issue report on our website here Version history for this manual 1 0 Initial release with Specifications 1 1 Removed Specifications Added cover page 1 2 Updated output pinout added mating connector part number 1 3 New functionality added 11 2013 1 4 Updated pinout images 5 2014 1 5 Overhauled Version 1 6 19 4 10 2015
17. t Setup Hardware Correlations 6 Turn off Headstage Press the Turn Off HS button in StimWare to turn off headstage when experiment is over The Headstage indicator will go gray when headstage is off Version 1 6 17 4 10 2015 STIMWARE User s Manual Channel1 V Channel2 7 Search HS Setup l Step1 Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS Step4 Select HS in list with mouse and click on Select HS Step5 Download pattern to selected HS or send cmd to it C 2Ch STIMWARE V1 7 0 Default Config File ini Download Tri Pattern ooga Clipping _ DC Offset Reset Clip a_i 9 22 2015 11 53 AM Off Command Ack 9 22 2015 11 53 AM Off Command Downloaded 9 22 2015 11 34 AM Set Trigger Ack 9 22 2015 11 34 AM Set Trigger Downloaded 9 22 2015 10 29 AM Trigger Command Ack 9 22 2015 10 29 AM Trigger Command Downloaded Figure 25 Turning Off Headstage Dongle Headstage Pattern hala Application Notes Charging the Headstage Lithium lon Battery The wireless stimulator headstage includes an integrated rechargeable battery which should be recharged when not in use Recharging is accomplished by the following procedure 1 Connect supplied charging plug to the charge port on the headstage 2 The LED on the charging plug will turn green when the battery is charged Electrodes Microprobe
18. trigger The pattern will start after delay T2 If the negative pulse T1 is less than 4ms it s regarded as false trigger and ignored and the pattern won t start T3 is interval between the end of pattern and the next coming trigger edge User should set up the reasonable pattern duration by considering T4 which is interval between the two consecutive trigger pulses Version 1 6 15 4 10 2015 STIMWARE User s Manual T1 I VH Trigger Signal Pattern Start Pattern Stop Falling edge 4 T3 gt T4 p Figure 22 External Trigger Falling Edge Active Parameter Desoiption S ae E B p _ Po Eoo ooo i O Po a a a S T3 pattern end to next trigger pattern end to next trigger edge time time a nae _ AA a a aa Figure 23 External Trigger Table 5 View stim pattern Confirm the signal shape coming out of the headstage with an oscilloscope or similar device Either pressing Trigger button in StimWare or using external trigger signal connected with Dongle will start the pattern that is already loaded on the headstage The entire pattern will execute once for each valid trigger and then the headstage will go into standby mode Version 1 6 16 4 10 2015 STIMWARE User s Manual e Test Setup oe Comme p no Kine go TS f jm m f aar a eS ld a DULL E W I Stimware USB Dongle Oscilloscope Head Stage on Test Board Figure 24 Tes
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