HOW TO USE AES2007
Contents
1. Z SEMIAUTOMATIC ELECTROPHORESIS SYSTEM AES 2007 USER MANUAL The Hellabio AES2007 system is intended to be used for electrophoresis on Agarose gels The System automates the most critical steps of electrophoresis to save time to get precise results and to make electrophoresis easier and more practical Code HM 001 ELECTROPHORESIS By AES2007 SYSTEM IMPORTANT NOTES You must e Be technical trained personnel to work with this system e Follow thoroughly the instructions e Donot exceed the maximum operating voltage e Use only distilled water to prepare working solutions AES FRONT SIDE i TE B 2 10 202h 2 17 1 IN In of distaining solution 2 OUT Out of waste distaining solution 3 In and Out of staining solution 1 Electrode carrier on the Application area 2 Sample dilution plate 3 Temperature 4 Electrophoresis program to select 1 10 5 ON OF of the system 6 Ventilation 7 8 Gel carrier 9 Staining Distaining bath 10 Enter of Electrode Carrier 11 Volts 12 Time 13 Up Down of time 14 Volt regulator 15 Start of Staining Distaining process 16 Clear the former step 17 NORMAL STAIN BUTTON 18 IN of Saline in case of IFE 19 IN OUT of Staining Solution 20 Select program 21 Clear previous function 22 Start Stop of electrophoresis AES ACCESSORIES Sample Application System f SAMPLE TEMPLATE CARRIER oonne orcs i tit lt x anal a 2e C2. and insert it in the staining distaining bath Bheeeccess Seeeeeace 4 Press CLEAR and 5 then Press Button SB Automatically the gel will be washed dried and will be ready for analysis How to discard and renew the staining and or distaining solution from the tank 1 In case you have chosen the staining of the gels by staining sheets you have to renew the distaining solution in the tank after 10 distaining processes 1a Turn on button NORMAL STAIN in STAIN Put the INK tube in the waste bottle and press CLEAR OUT The distaining solution goes out After that press CLEAR put the INK tube in clear distaining solution and press IN to fulfill the tank with new solution 2 In case you have chosen the staining of the gels by staining solution you must renew the staining solution in the tank after 10 staining processes 1a Turn on button NORMAL STAIN in STAIN Put the INK tube in the waste bottle and press CLEAR OUT The distaining solution goes out After that press CLEAR put the INK tube in the new staining solution and press IN to fulfill the tank with new staining solution IMMUNOFIXATION under constraction For Immunofixation select program 8 For sample application use Sample Template 24 Go on the steps of electrophoresis point 1 29 After point 29 of 2 Adjust the antisera 3 and apply it on the electrophoresis it template on the electrophoresis area clinks and the Sample templa temperature carri
3. press it by forefinger and add on it 2 5 ml stabilizing solution 3 During the drying staining process al a wy 3a Control the Waste bottle to be empty 4 Press IN Distaining solution enters a l l _ in the tank it is inside of the system 3b Control that the Distaining bottle contains enough solution 10 Acetic _ l oo Seid as soon as it is fulfilled it clinks This distaining solution will be reused in 3c Change NORMAL STAIN button in STAIN The green lamp is displaying each case and must be discarded after 10 electrophoresis see point 5 Return NORMAL STAIN button in 6 After 8 minutes remove the 7 Take the dried gel adjust it on the NORMAL staining sheet and let the gel Film carrier and put it in the distaining drying At the end of the bath process it clinks 8 Press CLEAR and 9 then Press Button SB Automatically the gel will be washed dried and will be ready for analysis 1 During the gel is drying Turn NORMA STAIN button in STAIN put the 2 Return the NORMA STAIN button in INK tube in the staining solution and press IN The staining solution enters NORMAL The staining solution is in in the tank inside of the system As soon as it is fulfilled it clinks the tank inside of the system and is enough to stain 10 gels To discard it see point 3 After electrophoresis point 23 and after the end of drying process it clinks Take the gel and adjust it on the Film carrier
4. er increases until about 30 C Blot quickly the surface of the gel by one gel blotter sheet 4 Press ENTER The 5 Apply the protein 6 After 10 minutes antisera template fixation solution and press CLEAR steps down the antisera on the correct position
5. is _ Qa xt o SAMPLE AP CATOR 24 i Sample Application System consists of the Sample Template carrier the Sample template and the Sample Applicator Sample template included in AESOO7 and in Sample Template Carrier included in AEU01 AESOO7 and in AEUO1 Buffer sponges included in Sample dilution Bottles for distaining solution KIT for AES2007 and AEU01 Plate included in and waste liquids included in AES2007 and in AEUO1 AES2007 and AEU02 Gel Carrier INSTALATION e Connect the system in electricity 220vOlts e Connect the tubes IN OUT INK on the back side of the system PREPARATION OF THE REAGENTS e PREPARATION OF THE BUFFER Follow the instructions on the bottle e PREPARATION OF THE STAINING SOLUTION Follow the instructions on the bottle e PREPARATION OF THE DISTAINING SOLUTION Prepare 10 acetic acid solution Add to 900 ml dest water 100ml clasial acetic acid and mix it STEPS OF ELECTROPHORESIS 1 Turn on the system 2 The lamps of temperature volts and time are displaying 4 Add to each sponge 6ml buffer and let it 5 Prepare the samples 6 Add 200ml dest water on the 2 3 minutes to be absorbed dilution electrophoresis area 9 Adjust the Sample Template on the carrier 11 Gently press with forefinger the sample template 12 apply the samples on the corner of the basin near the and Slits 13 Adjust the sample applicator on the sample 14 in 1 minute
6. press Clear template and 15 Remove the carrier template applicator Uy wir T d a i 16 Apply the electrode carrier sponges on the electrophoresis area wese 17 Select program 18 Press ENTER 19 and control the correct 20 According to the selected program position of the electrode carrier the volts and time are displaying 21 Press START 22 The red lamps on the 23 At the end of electrophoresis the electrode carrier are blinking electrode carrier steps upstairs and it clinks Remove the electrode carrier TAKE ATTENTION Automatically starts the drying of the gel You have to decide the way of staining In case of Immunofixation see section IMMUNOFIXATION During the electrophoresis you have to decide the staining way and go to prepare the steps see staining Process or Il The staining of the gel can be done 1 By using ready to use staining sheets during the drying of the gel or 2 By using of staining solution in the staining distaining bath By the first case using staining sheets you save the staining time because the staining occurs on the same time with drying and there is no need to use staining solution STAINING PROCESS I STAINING PROCESS by using ready to use staining sheets 1 Immediately after electrophoresis and as soon as the electrode carrier steps upstairs 2 remove the electrode carrier apply one staining sheet on the gel slightly
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