LumenLED
Contents
1. The LED units are designed to be mounted with the shortest wavelength nearest the microscope frame See Fig XX LumenLED WO 3 3 2 Installing Dichroics into Mirror Holder Fig 7 Dichroic Mirror Mirror Fixing Spring Locating Notch To add a new dichroic mirror or to change one e Pull back the mirror fixing spring and place the mirror flat with the surface of the holder e The sides of the dichroic mirror should sit flush with the bottom and side of the hoder e Slowly release the spring and this will firmly hold the mirror in place NOTE When mounting a Dichoric mirror inside the holder always make sure that the reflective coated side of the Dichroic mirror is facing the lightsource 3 3 3 Installing the Dichroics in to the LED Main Housing The dichroic mirror must be mounted in the corrected orientation inside the LED main housing To aid for this a locating notch has been added to the mirror holder e Remove the 5 screws holding down the cover on the main housing e Insert the mirror holder into the relevant slot inside the main housing e Remember that the lowest wavelength mirror must be closest to the microscope e Make sure the mirror holder is seated firmly in place with the locating notch at the bottom e Replace the cover and 5 screws of the main housing Fig 6 Orientation of dichroic mirrors The Dichroic mirrors must be mount in the correct order The shortest wavelength must be closest to the2. CAN Connector TTL Out Connector Note the above is an example of a LumenLED 4 LED system TTL 3 and TTL4 plus LED 3 and LED4 ports are not available on a LumenLED 2 LED system It is possible to add an extension unit to convert a LumenLED 2 LED system into a LumenLED 4 LED system please contact your local Prior representative see contact details in section 10 8 LumenLED 3 3 Installing the system All the connectors are labelled when plugging and unplugging connector always turn the power off 3 3 1 Installing LEDs It is best to install the LEDs into the LED main housing LDXXX before installing the system to the microscope Fig 4 LED Main Housing Fig 5 LED Cover Locking Screws NOTE Dichroic Mirror Locking Screws Ensure the controller is switched off and is not connected to the power Connect the LED ports on the LumenLED controller to the LED units using the cables provided see Fig 3 It is not important which LED connections are used on the Controller but this should be noted for later use Fix the round connector to the LED unit making sure to align the notch in the connectors and push together Screw the outer casing to lock the connector in position Remove any covers on the LED main housing see Fig 5 Loosen the LED fixing screws Align the grove in the LED unit with the fixing screw of the main housing Gently push in the LED unit Tighten LED Locking screw to secure LED units
3. 18 LumenLED Fig 28 E Found New Hardware PRIOR Virtual COM Port X G _ Found New Hardware PRIOR Virtual COM Port Search for driver software in this location CAUsers yourname Desktop Prior PS3 USB Driver M Include subfolders Click Browse and browse to the location of Prior PS3 USB Driver folder click NEXT Fig 29 R Windows Security x x Windows cant verify the publisher of this driver software Dont install this driver software You should check your manufacturers website for updated driver software for your Install this driver software anyway Only install driver software obtained from your manufacturers website or disc Unsigned software from other sources may hamn your computer or steal information See details Click Install this driver anyway Click Finish 19 LumenLED 4 1 Installing the Prior Communications Port for USB operation Windows 7 Copy the folder Prior PS3 USB Driver to your computer Plug in the USB cable to the PS3 and computer Switch the PS3 on The computer will detect the new USB connection and the following popup window will appear How do you want to search for driver software Browse for driver software on your computer Search for driver software in this location gt Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device unless you ve disabled this feature in your
4. ENGLAND CB1 5ET Tel 01223 881711 Fax 01223 881710 email uksales prior com Prior Scientific KK 3F Metlife Kabuticho Bldg 5 1 Nihonbashi Kabuto Cho Chuo Ku Tokyo 103 0026 JAPAN Tel 81 0 3 5847 8213 Fax 81 0 3 5847 7901 email info japan prior com Prior Scientific Inc 80 Reservoir Park Drive Rockland MA 02370 1062 USA Tel 781 878 8442 Fax 781 878 8736 email info prior com 31 Prior Scientific GmbH Wildenbruchstr 15 D 07745 Jena GERMANY Tel 49 0 3641 675 650 Fax 44 0 3641 675 651 email jena prior com LumenLED APPENDIX I LumenLED and Compatibility Mode There is an option to run the LumenLED in Compatibility mode This mode allows the system to emulate a Proscan III with filter wheel and shutter capability and therefore makes the system compatible with software currently supporting Prior filter wheels and shutters LED COMP I When changing from the default standard mode to the compatibility mode the system must be restarted LED wavelength selection will emulate one filter wheel and LED intensity level will emulate another filter wheel The will be one filter wheel position left to add an additional physical filter wheel Eg Filter Wheel I HF110 filter wheel Filter Wheel 2 LED wavelength selection Filter Wheel 3 LED Intensity Level Selection Note as the Prior filters wheels a designed with 10 positions the possible intensity levels will be as foll
5. expand the PORTS option The connection label Prior Communications port is the PS3 connection Note the COM port associated with it 16 LumenLED 4 1 3 Installing the Prior Communications Port for USB operation Windows Vista Copy the folder Prior PS3 USB Driver to your computer Plug in the USB cable to the PS3 and computer Switch the PS3 on The computer will detect the new USB connection and the following popup window will appear Windows needs to install driver software for your PRIOR Virtual COM Port ie Locate and install driver software recommended Windows will guide you through the process of installing driver software for your device Ask me again later Windows will ask again the next time you plug in your device or log on i Don t show this message again for this device Your device will not function until you install driver software Click Locate and install driver software Fig 25 EL Driver Software Installation Installing device driver software PRIOR Virtual COM Port Searching Windows Update This window may appear at the bottom of your screen walt The following window will appear 17 LumenLED Fig 26 L Found New Hardware PRIOR Virtual COM Port il Click I don t have the disc Show me other options The following window will appear Fig 27 L Found New Hardware PRIOR Virtual COM Port Click Browse my computer for driver software Advanced
6. limited assuming the LED is not running in the Overdriven Zone 6 4 Overdriven Zone The Overdriven Zone is available for applications where a high light intensity is required for short burst of time When the power is set to greater than 100 the on time of the LED is limited to a maximum of 20ms The subsequent OFF time is calculated as a duty cycle percentage from the power setting Eg If the power is at 120 then the OFF time has to be a minimum of 4ms The restriction in the ON time is automatically enabled to protect the LED from damage due to the high power input in overdriven mode 26 LumenLED SECTION 7 SOFTWARE COMMANDS LED NONE Queries the information about LED n END Or FLUOR UV LAMBDA 405 SERIAL 12345 END LED COMP LED COMP 0 Standard mode LED COMP I Emulation Mode LED COMP give the mode status LED n n L C 0 CL a L for Light Mode MODE a a C for Current Mode LED n MODE responds with operational mode C or L LED n Queries the state of the LED STATE a Responds on 0 off Queries the LED Power a selected operating power b percentage by which LED can be over powered Eg 100 120 Sets the LED power to p percent The value of p can be up to the max percent the LED can be over powered ie b above H E L D a b Queries the current TTL settting H Trigger High E Trigger Enabled L Trigger Low D Trigger Disabled a msec ON time b msec OFF time
7. 0 LumenLED SECTION 7 SECTION 7 SECTION 8 SECTION 9 SECTION 10 APPENDIX 1 SOFTWARE COMMANDS REPLACEMENT amp SPARE PARTS SERVICING TECHNICAL SPECIFICATIONS RETURNS AND REPAIRS LumenLED AND COMPATIBILITY MODE 28 30 31 31 32 33 LumenLED SECTION 1 IMPORTANT SAFETY INFORMATION SAFETY WARNINGS Always observe the following safety precautions A This symbol used on the LED units and in this manual means Caution refer to accompanying documents Before using the illumination system please follow and adhere to all warnings safety and operating instructions located on the product and in this User Manual Use only as specified by these operating instructions or the intrinsic protection provided by the unit may be impaired It is safe for use in an ambient temperature from 5 to 40 C with relative humidity to 80 up to 31 C decreasing linearly to 50 rH at 40 C Do not expose the product to water or moisture while energised Do not allow moisture to penetrate openings in the case If this occurs allow the unit to dry out unpowered before switching on again Do not expose the product to extreme hot or cold temperatures Do not expose the product to open flames Do not allow objects to fall on or liquids to spill on the product Do not replace detachable mains supply cords by inadequately rated cords Do not cover or impede ventilation to this system Do not stare into the light outp
8. 0nm Additional Dichroic Mirrors LDD410 LDD495 LDD552 LDD593 Long pass filter 410nm Long pass filter 495nm Long pass filter 552nm Long pass filter 592nm 29 LumenLED SECTION 9 SERVICING This equipment contains no user serviceable parts Refer all repairs to qualified service personnel Opening the product voids the warranty For approved service outlets contact your nearest Prior office SECTION 10 TECHNICAL SPECIFICATIONS e This equipment is for indoor use and is designed to be safe within an ambient temperature range 5 to 40 C with maximum relative humidity of 80 relative humidity derated linearly to 50 rh above 31 C e Dry weight 6 1Kg e Dimensions Not including tip support bracket 174mm W x 130mm H x 250mm D e Supply voltage 100v to 240v 50Hz to 60Hz 30 LumenLED SECTION 11 RETURNS AND REPAIRS Should you experience problems with your LumenLED System and want to send it back for service warranty or otherwise a Return Material Authorisation RMA number must be obtained from the appropriate Prior Scientific office before returning any equipment For North and South America contact Prior Scientific Inc for Japan contact Prior KK for Germany Austria and Switzerland contact Prior GmbH and for the all other countries contact Prior Scientific Instruments Limited on the telephone numbers shown below Prior Scientific Instruments Ltd Unit 4 Wilbraham Road Fulbourn Cambridge
9. 1 of controller Button 2 On Off control of LED connected to LED Port 2 of controller Button 3 On Off control of LED connected to LED Port 3 of controller Button 4 On Off control of LED connected to LED Port 4 of controller Button 5 Menu Button only if connected to a Proscan ITI controller Button 6 Focus Control only if connected to a Proscan III controller Left Digipot Control of the Light Intensity Levels for selected wavelengths Right Digipot Control of the Focus Levels only if connected to a Proscan III controller 21 LumenLED 5 1 2 Control of LED Intensity via PS3J100 The intensity of each LED and be set individually via the PS3J100 in 1 steps from 0 to 100 e Press and hold down relevant LED button eg Buttons 1 4 e Rotate Left Digipot clockwise to increase intensity anticlockwise to decrease intensity e Release the relevant LED button eg Buttons 1 4 e To Turn off LED push the relevant button eg Button 1 4 to turn back on press the same button again 5 1 3 Display Screen of PS3J100 Fig 31 Display of PS3J100 The available wavelengths to control from the PS3J100 can be seen on the display screen of the PS3J100 LED OFF O LED ON 100 LED405 Intensity set at 100 405 Wavelength of LED attached to Port 1 of LumenLED controller 460 Wavelength of LED attached to Port 2 of LumenLED controller N A No LED attached to that particular Port 22 LumenLED 5 2 Using the Prior Demo Software to Contro
10. 23 Hardware Installation Fig 22 Found New Hardware Wizard Please choose pour search and installation options ned Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM Include this location in the search CADocuments and Settings w Don t search I will choose the driver to install Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for your hardware 4 The software you are installng for this hardware Prior Communication Port has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of pour system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation The Prior Communications Port will now installed Click Finish Your computer may ask you to repeat this process To Identify your Port Number e Got to Start gt control panel Select System from your control panel Select the hardware tab and then Select device manager In device manager
11. LED n TTL PP Enables Trigger E LED n TTL PP Disables Trigger D 27 LumenLED LED n TTL LED n TTL LED is on when TTL is HIGH H LED n TTL LED is on when TTL is LOW L LED n TTL Sets the LED ON time for pulsing T on Note if power lt 100 on times are not restricted If power gt 100 on times are restricted Examples of Typical Commands Example I Turns LED 1 ON and OFF via software commands at 100 power LED 1 POWER 100 sets power to 100 LED1 STATE 1 turns on LED LED 1 STATE 0 turns off LED Example2 Turns LED 1 ON and OFF via software commands at maximum power LED I POWER query power setting Software response 100 120 two values returned by controller The second is the maximum power LED 1 POWER 120 Sets power to 120 LED 1 TTL the LED cannot be permanently on when over driving the power Therefore query the pulse time R D 200 40 Controller switches to rising edge mode with on time of 20ms and then controller switches off automatically for 4ms before another command accepted LED 1 STATE 1 Start single pulse 28 LumenLED SECTION 8 REPLACEMENT amp SPARE PARTS Additional LED Units LED 365 Single LED Unit 365nm LED385 Single LED Unit 385nm LED405 Single LED Unit 405nm LED447 Single LED Unit 447nm LED460 Single LED Unit 460nm LED505 Single LED Unit 505nm LED525 Single LED Unit 525nm LED590 Single LED Unit 590nm LED625 Single LED Unit 625nm LED660 Single LED Unit 66
12. LumenLED Manual Version 1 00 Table of Contents SECTION 1 IMPORTANT SAFETY INFORMATION 1 1 IMPORTANT SAFETY INFORMATION SECTION 2 LEDS amp FLUORESENCE 24 Introduction 22 Switching Speeds and stability of LEDs 72 Availability of new LEDs SECTION 3 INSTALLATION 3 1 Identifying the parts of the LumenLED System 3 2 LumenLED controller box connections and indicators 3 3 Installing the system 3 3 1 Installing LEDs 3 3 2 Installing Dichroics into Mirror Holder 3 3 3 Installing the Dichroics in to the LED Main Housing 3 3 4 Installing Excitation filters 3 3 5 Installing LumenLED unit on microscope 3 4 Adjustments to LED 3 5 Connecting the Interactive Control Centre PS3J100 SECTION 4 INSTALLATION OF SOFTWARE 4 1 Installing Software 4 1 2 Installing the Prior Communications Port for USB operation Windows XP 4 1 3 Installing the Prior Communications Port for USB operation Windows Vista 4 1 Installing the Prior Communications Port for USB operation Windows 7 SECTION 5 WORKING WITH LEDS 5 1 Using the Interactive Control Centre to Control LED 5 1 1 Button functionality of PS3J100 5 1 2 Control of LED Intensity via PS3J100 5 1 3 Display Screen of PS3J100 2 Using the Prior Demo Software to Control LED 5 2 1 Control of LED Intensity via Prior Demo Software SECTION 6 MODES OF CONTROL 6 1 Current Mode 6 2 Light Mode 6 3 TTL Commands 6 4 Overdriven Zone NNN 14 14 15 17 2
13. device installation settings V Include subfolders gt Browse my computer for driver software Locate and install driver software manually gt Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device Select Browse my Computer for driver Software Installing driver software gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device gt Install this driver software anyway Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information v See details Install this driver anyway The drivers will be installed Windows has successfully updated your driver software Windows has finished installing the driver software for this device Prior Communication Port Device is now ready to use 20 LumenLED SECTION 5 WORKING WITH LEDS The LumenLED can be controlled manually via the Interactive Control Centre or via software 5 1 Using the Interactive Control Centre to Control LED Fig 30 PS3J 100 Control Centre PTE 7 ER OS PE HF nm em se Gi el Le Le Left Digipot Right Digipot w PRIOR gg 5 1 1 Button functionality of PS3J100 Button 1 On Off control of LED connected to LED Port
14. e the LED units to be overdriven up to a maximum of 120 however if this feature is enabled the length of ON time is restricted to prevent damage to the LED see section 6 4 Fig 35 LED 1 NAME 447nm TTL Triggers Power COn Off On High lt 120 pulse Time ms 20 0 owe Power Zone In Light Mode the light intensity of the LED can be controlled via a closed loop optical feedback system This ensures that the intensity of the illumination is kept constant throughout the course of the experiment To allow for the optical system to work the maximum power of the LED has to be reduced slightly to allow the system to regulate the illumination levels Therefore it is not possible to work in the Overdriven zone so the Power Bar is limited to 100 6 2 Light Mode 25 LumenLED Fig 36 LED 1 NAME 447nm TTL Triggers Power On OF fe On High JL pulse Time ms 20 6 3 TTL Commands TTL commands can operate in either Current mode or Light mode For each LED unit there is one TTL BNC connector It is important to note that Trigger commands override any software commands Therefore if the software has switched the LED off but the Trigger 1s enabled and the correct state eg High defined then the LED will come on despite what the software says The length of time the LED is ON depends of the time specified in the PULSE TIME box This value is set in milliseconds and the time set is not
15. etup if you are asked by windows to allow the program to run click yes allow or run Fig 15 Fig 16 i Prior Scientific Software InstallShield Wizard i Prior Scientific Software InstallShield Wizard Welcome to the InstallShield Wizard for Prior Ready to Install the Program Scientific Software The wizard is ready to begin installation The InstallShieldtR Wizard will allow you to modify repair or If you want to review or change any of your installation settings click Back Click Cancel to remove Prior Scientific Software To continue click Next exit the wizard Current Settings Setup Type Destination Folder C Program Files Prior Scientific Components User Information Name Company Installshield Click Next Click Install Fig 17 ir Prior Scientific Software InstallShield Wizard InstallShield Wizard Completed The InstallShield Wizard has successfully installed Prior Scientific Software Click Finish to exit the wizard TT Click Finish 14 LumenLED Fig 18 tg Prior Scientific Software Installer Information x changes made to Prior Scientific Software to bake effect Click Yes to restart now or No if vou plan bo restart later AN You must restart your system For the configuration Click Yes to restart your computer immediately Click No and restart you computer before using the program 4 1 2 Installing the Prior Communications Port for USB operation Windows XP Copy t
16. he folder Prior PS3 USB Driver to your computer Plug in the USB cable to the PS3 and computer Switch the PS3 on The computer will detect the new USB connection and the following popup window will appear Fig 19 Fig 20 Found New Hardware Wizard Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by This wizard helps you install software for looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission USB Serial Converter Read our privacy policy Can Windows connect to Windows Update to search for OR ee f ng ri atten aa an installation CD software PP j O Yes this time only Yes now and every time connect device What do pou want the wizard to do Install the software automatically Recommended Click Next to continue Click Next to continue Select No not at this time then click NEXT Select Install from a list or specific location advanced The following screen will be displayed Browse to the location of Prior PS3 USB Driver folder on your desktop click NEXT 15 LumenLED Fig 21 Browse For Folder My Documents E F My Computer E s t VAIO C DO Documentation 5 Documents and Settings E DO Drivers Gy Audio ID Bluetooth ID Camera ID Chipset or a To view any subfolders click a plus sign above Click Continue Anyway Fig
17. l LED Run the Prior Software from Startmenu gt Program gt Prior Scientific gt Visual Basic gt Controller Demo Fig 32 Port Selection Please enter the COM Part or 0 for FCI OK Cancel Enter the number of the COM port the ProScan controller is attached on Tip To find the virtual COM port use Startmenu gt Control panel gt system the click the Hardware tab then the Device manager button expand the ports section to identify and virtual COM ports The Following screen will be displayed Fig 33 PriorDemo software _ Prior Test Control Motor Speed Backlash Encoders Reference Correction Stage Piezo TTL LEDS Help DRO Measure Be Fe Joystick Status Filters 4th Temperature Voltage Moving Limits Only attached accessory sections will be active e g if there are no shutters all of the Shutter buttons in the Shutter area will be light grey and inactive In the Fig 33 above no other device other than the LEDs have been connected Control of the LED units can be found by clicking the LED Menu and select SHOW LEDS The following control box will appear for each wavelength attached to the system in the example LED 447nm 23 LumenLED Fig 34 PriorDemo LED Control Module gt LED 1 NAME 447nm TTL Triggers On C Off Light Mode Deu MG 0 100 100 High Low gt gt gt pulse Til Rising Edge N Falling Edge From this control box several options are available for the as
18. microscope The direction around indicated the direction of wavelengths from longest ie Red towards shortest eg Blue 10 LumenLED 3 3 4 Installing Excitation filters Should you wish to optimise the bandwidth of the LED to the appropriate dye you may want to add excitation filters The LED units are designed so that they allow for the inclusion of 25mm filters Make sure the controller 1s switched off and remove LED units Use the Prior Filter Block tool to locate the two holes of the filter mounting ring Remove the filter mounting ring and insert the filter into the LED unit Replace the filter mounting ring to secure excitation filter into LED unit NOTE It is important to make sure you have orientated the filter correctly This can change depending on manufacturer of the filter Fig amp Excitation filter and fixing ring Fig 9 Excitation filter F SO 3 3 5 Installing LumenLED unit on microscope It is recommended that Dichroic mirrors be installed before mounting the LED main housing on to a microscope The LumenLED is supplied with an appropriate mounting adapter specifically designed to fit the microscope the supplied mounting adapter will only fit the specified make of microscope Adapters can be exchange should you wish to move the LumenLED onto another manufactures microscope 3 3 6 Adjustments to LED To optimise the performance of the LED it is possible to focus and centre each i
19. nd Position P text 7 1 T 3 Dapi will set the tag for filter wheel position 3 to Dapi Tags are 6 characters log and are displayed in control centre display 33 LumenLED LED Shutter Emulation Commands R If LED s is not fitted E 20 will be returned C If LED s is not fitted E 20 will be returned Text string Turns ON or OFF the LED s value I 2 or 3 if cis 0 the LED is ON I it is OFF The optional argument t is used to ON OFF the LED for a time t milliseconds First parameter is a zero Subsequent parameters define the startup state of LED s1 s2 and s3 respectively if si is 0 then LED I is ON I it is OFF etc Default state is all LED closed Returns status c of LED s Prints information about LED s s is a value between and 3 The information end is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines until the END is seen in order to maintain compatibility Example SHUTTER I NORMAL DEFAULT STATE CLOSED END 34 LumenLED
20. ndividual LED unit The LumenLED system must be fully mounted onto the microscope and the appropriate microscope filters used for the relevant LED units before they can be correctly adjusted and the illumination optimised 11 LumenLED Fig10 Focus Locking Screw To Focus the LED units Select the appropriate microscope filter and turn on the LED unit Focus the microscope onto a suitable sample such as Autofluorescent Plastic Slides 92001 from Chroma Technology Corp Using Allen Key to undo the focus locking screw see Fig10 The top half of the LED unit will now freely slide back and forth along the direction of the blue arrows Moving the top of the unit away from its base with spread the illumination across the field of view Moving the top of the unit will focus the illumination into smaller area increasing the intensity but reducing the homogeneity Once the optimum position has been achieved lock the focus locking screw Fig 11 Location of Locking Screws Centring Locking Screws 12 LumenLED To Centre the LED units Select the appropriate microscope filter and turn on the LED unit Focus the microscope onto a suitable sample such as Autofluorescent Plastic Slides 92001 from Chroma Technology Corp Using an Allen Key unlock the 3 centring locking screws see Fig 11 The top half of the LED unit will freely move in a circular motion Look at the image and check the flatness of illumination Fig 2 sho
21. oduct Do not stare at operating lamp May be harmful to the eyes WARNING If PAT tester applies AC dielectric strength test this may fail due to EMC filters and a DC test is preferable gt WARNING Do not repeat the use of dielectric strength testing as this may damage safety insulation Save this manual as it contains important safety information and operating instructions 4 LumenLED SECTION 2 LEDS amp FLUORESENCE 2 1 Introduction Traditional method of microscope illumination involved the use of a white light source such as mercury or xenon arc lamps These are broad spectrum lamps and require the use of optical filters to block out unwanted wavelengths However no filter s 100 efficient meaning some unwanted light will always reach your specimen A light emitting diode LED is a semiconductor device that emits a narrow band of light when a current is applied to it The colour of emitted light depends upon the composition of the semiconducting material used and can range across the spectrum from near ultraviolet to infrared Varying the composition of the semiconducting material used by the introduction of impurities to the silicon is known as Doping FIG 1 Different LED wavelengths TIE MO N ff TH HN IVA UOT TA HM TT TAL TRAV TITT TL TAN IT TH TALITY A TEILT TI TUTE VL TT TT TEV g y LEIL AVE VNVT VEN UTV NA VE TT IM I XA HAN N Gern Normalised a 3 co Although the spectral ou
22. ows l 100 95 90 85 80 75 70 65 50 0 25 O O0 I ON EU FB D The ON OFF function of each LED will emulate the Prior shutter If a physical shutter is present also select one of the available free shutter ports for the LumenLED Eg Shutter I HF202 shutter Shutter 2 LumenLED ON OFF function Shutter 3 Free The Open command of the shutter will turn On the LED unit currently selected in the filter wheel The Close command of the shutter will turn OFF the LED unit currently selected in the filter wheel 32 LumenLED LED Filter Wheel Emulation Commands ker 7 w f Rora w defines the LED wavelength number 1 2 or3 f is number If defined below LED is If f is a number command moves filter LED w to LED fitted E 17 position f will be If f is N command moves LED w to next LED returned If f is P command moves LED wheel w to previous LED If f is F command reports current LED position on If f is H command performs a home routine No effect O f1 f2 f3 The first parameter zero indicates all LED the three subsequent parameters fl f2 and f3 are the target positions for LED I 2 and 3 respectively LED not fitted or invalid filter wheel positions are ignored This feature is only available in COMP 0 mode TE LP Text Displays tag text for LED w at position p 7 1 T 3 will respond with text for LED I position 3 BE F Writes text to memory for LED W a
23. sociated LED unit Selection of LED operation mode Light Mode or Current Mode see section 5 Turn ON or OFF Intensity levels set in 1 intervals from 0 to 100 Control of the LED units via TTL Triggers can be turned ON or OFF The pulse time for LED on time via the TTL signal The status of the TTL trigger can be set see Fig 34 5 2 1 Control of LED Intensity via Prior Demo Software The intensity of each LED and be set individually via the Prior Demo software in 1 steps from 0 to 100 e Left click and hold mouse on the Power Bar e Mouse Power bar towards the left 0 to decrease power and towards the right 100 to increase power e To turn of LED use the On Off radial buttons 24 LumenLED SECTION 6 MODES OF CONTROL The LumenLED can operate in various modes and the user is freely able to select the mode most appropriate to their particular application Although in the short term the light output from an LED tends to be more stable than that from other sources it 1s somewhat temperature dependent To correct for this the option of optical feedback to stabilize the output is also provided this is known as Light Mode It is also possible to set up each individual LED unit to work in different operational modes See list of HyperTerminal commands 6 1 Current Mode Current Mode is the default setting for the LumenLED system In thix mode the system is able to run at up to 100 of its power It is also possible to enabl
24. tput of an LED is more specific than a standard arc lamp it may still be wide enough to require the addition of an excitation filter to optimise the band width for the particular wavelength Please see section 3 3 4 Installing Excitation Filters It is important to match the correct LED with the appropriate filter sets please see contact your local Prior office for advice LED illumination has various advantages over traditional delivery method of illumination With LED technology there is little heat generated allowing the illumination to be directly coupled to the microscope to maximise light while avoiding excessive heat transfer to the specimen Excitation filters if required can be mounted directly in front of the LED avoiding the need for excitation filter wheels and therefore also avoiding unwanted vibration in the system 5 LumenLED 2 3 Switching Speeds and stability of LEDs Typically a filter wheel and shutter system can switch between wavelengths in around 80milli seconds This LumenLED will switch in micro seconds when connected via TTL analogue control The stability of light sources varies but HBO bulb will decrease by 30 50 over 200 hours a metal halide will decrease 30 over 2000hours Both have short term stability issues Included in the LumenLED is a closed loop feedback system to ensure the light is stable and reliably the same each time the unit 1s powered up This closed loop feedback keeps the light the same no ma
25. tter how long or short a time scale the system is measure over LEDs can last for over 20 000hrs 2 4 Availability of new LEDs New wavelengths and higher power LED are being generated all the time if you have a specific application and cannot find the LED required as your local prior office for more information 6 LumenLED SECTION 3 INSTALLING YOUR SYSTEM 3 1 Identifying the parts of the LumenLED System The controller consists of I off controller box I off RS232 Cable I off USB cable I off Power cable supplied suitable for you geographical area I x Main Housing either a four or two LED version LED unit LED cables one per LED Unit The controller box may be of two variants the vertical stack option or the horizontal stacking options for the purposes of this manual we will use the vertical version in pictures and instruction below however the instructions apply equally to the horizontal version Fig 2 Front of LumenLED Power Indicator Tx RX indicator Status Indicator 3 2 LumenLED controller box connections and indicators The controller front face has 3 LED POWER Green Illumined Power On TX RX Two Colour LED RX receive and TX transmit communications RX Amber illuminated TX Green illuminated STATUS Red illuminated Fault in system 7 LumenLED The rear of the controller has the connections to LEDs TTL communications and power Fig 3 Back of LumenLED TTL In Connector
26. ut of the LED units Connect the AC power cord only to designated power sources as marked on the product Make sure the electrical cord is located so that it will not be subject to damage Make sure the system in installed so that the reaer panel power switch 1s easily accessible For use in a manner not specified in this manual contact Prior before any work is done To reduce the risk of damage unplug the product from the power source before connecting the components together DANGER never alter the AC cord or plug If the plug will not fit into the outlet have a proper outlet installed by a qualified electrician Only suitably rated and approved mains cord sets should be used as per the country of use Use only the proper type of power supply cord set provided with the system for this unit The LumenLED is class 1 and must be only connected to a power outlet which provides a protective earth ground Fuse Rating T2A 250v Power Rating 150W 3 LumenLED Do not attempt to disassemble the product Doing so will void the warranty This product does not contain consumer serviceable components Service should be performed by Authorised Service Centres Only the exterior of this product should be cleaned using a damp lint free cloth This warning symbol indicates that there is a high voltage danger This warning symbol indicates that the surface may be hot CAUTION Possibly hazardous optical radiation emitted from this pr
27. ws 4 images with uneven illumination in each corner Carefully adjust the position of the LED until you achieve a homogeneous illumination across the field of view See Fig 13 Fig 12 Examples of incorrect adjustment Fig 13 Homogenous illumination To achieve the best results it may be necessary to repeat the above steps of focusing and centring the illumination 3 3 7 Connecting the Interactive Control Centre PS3J100 Thelnteractive Control Centre PS3J100 can provide control of the ON OFF functions as well as the illumination intensity levels of each of the LED units installed To Install the Interactive Control Centre PS3J100 e Ensure the system is switched off e Plug in the 9 way connector to either the RS232 1 if this is not available use the RS232 2 connector e Power on the system e The Interactive Control Centre will detect the ProScan III controller and associated peripheral devices and automatically configure the internal software 13 LumenLED SECTION 4 INSTALLATION OF SOFTWARE 4 1 Installing Software 4 1 1 Installing the Prior Freeware All of the Prior hardware can be controlled with the PriorDemo software The software is available to download from http www prioruk com downloadcentre dc_software html Content of the Zip file Fig 14 Prior Software Disc W zip Pile Edt Yew Favores Took Hep QO Q BF Poh Py rates s ress DI Wii dowrloadcertre software Price Software Disc Va 2p Run S
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