Hardware User`s Manual
Contents
1. Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B FCC FCCA47CFR 15B Class B Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can incorporate the CE marking and FCC C En cons quence ce produit peut incorporer le marquage CE et FCC En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canalis ae OS SS General Manager _A Panlab s l u a division of Hakvard BioScience Cornella de Llobregat Spain 25 06 2014 Behavioral box and load cell control unit 34 Harvard A Apparatus Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment taf Nota sobre la protecci n medioa2. 13 PREVENTIVE MAINTENANCE EXPERIMENT MONTHLY TRANSPARENT DOOR CLEANING CHECK GRID AND TRAY PLACING Only when you work with Startle experiment Behavioral box and load cell control unit Harvard Apparatus 31 Panlab H Medis 14 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum power Conducted noise 115 230 VAC 50 60 Hz 2 fuses 5x2omm 1A 250V Fast 60W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing SOUND AMPLIFIER Power supply Max current Input sensibility Input impedance Output impedance Frequency range Distortion 1W Distortion max power Power R M S Power musical Slew rate Linearity error Output ranges Range adjust amplitude 32V 1 5A 10V 20Vpp 820kO 1320KQ 80 20 20000HZ 0 01 1 60 W 85 5 W 15V us 190 in 10 steps 25 respect central point 100 to 63 respect max BRIDGE AMPLIFIER Power supply Max current Distortion max Slew rate Linearity error Gain ranges Low pass filter High pass filter Balance Zero Output full scale Range adjust load cell 12V O0 1A 0 001 5V us lt 0 1 200 500 1000 2000 5000 0 2 Hz 0 5 Hz 1 Hz 22 Hz 500 gr 2V a 2V 5096 respect central poin
3. afterwards remove the foam with a wet cloth and finally dry it with a dry cloth Behavioral box and load cell control unit 20 Panlab m Medis 9 7 WALLS CLEANING To clean the walls a slightly wet cloth can be used afterwards it should be dried with a dry cloth If the walls are too dirty it can be cleaned with a cloth wet in a soapy solution afterwards remove the foam with a wet cloth and finally dry it with a dry cloth Behavioral box and load cell control unit 21 Panlab HA tev 10 HOWTO INSTALL THE LE 115 CONTEXTUAL KIT The contextual kit is an accessory provided with the Panlab combined Startle amp Freezing system STARTFEAR for changing the context of the box when running Cue dependent Fear Conditioning experiments 10 1 COMPONENTS e White under grid base e Smooth floor e White walls e Transparent cylinder 10 1 1 WHITE UNDER GRID BASE The white under grid base is used to change the colour of the floor base when the user wishes to keep the grid during the test phase ofthe Cue dependent Fear Conditioning experiment Figure 14 White under grid base 1 Remove the grid from the tray 2 Place the white flexible floor on the floor of the tray 3 Place the grid on the tray again 4 Place the tray grid set in the box 10 1 2 SMOOTH FLOOR The smooth floor is used to change the texture of the floor during the Test so that the box can provide a different tactile cue to the subject As no s
4. basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Behavioral box and load cell control unit 4 Panlab H Medis 4 EQUIPMENT INSTALLATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user H Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight l A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibi
5. o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente I lt Nota em Protec o Ambiental Ap s a implementa o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuic o para a protecc o do ambiente D Behavioral box and load cell control unit 35
6. producto Load Cell Coupler Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 111 LE 116 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons applique les normes harmonis es suivantes
7. transparent as shown in some of the photographs Behavioral box and load cell control unit 26 Panlab H Medis 11 LE119 AIR PUFF ACCESSORY 11 1 CONNECTING THE ELECTROVALVE TO THE EXPERIMENTATION CAGE In the left side wall of the experimentation cage there is a headless screw it must be unscrewed and replaced by record 2 Afterwards the stainless steel tube 1 must be introduced in mouse model the bended part is longer than in rat model Then insert the plastic cone 3 in the tube and once the tube 1 is correctly positioned with respect to the restrainer screw the nut 4 The stainless steel tube 1 must be at least 1 5cm out of nut 4 so that it can be connected to the electrovalve 5 When connecting the cage to the electrovalve there are two options One is to connect the nut 4 directly to the electrovalve Option A The other one would be to place the electrovalve far from the experimentation cage in this case and additional connection should be made between the electrovalve 5 and the nut 4 Option B Option A Connect the electrovalve 5 directly to tube 1 OPTION A Behavioral box and load cell control unit 27 Panlab H Meise Option B Place the electrovalve far from the experimentation cage o OPTION B 11 2 COMPONENTS When the LE119 module is sold it comes with the following components e iElectrovalve with its connection cable e 2 Stainless steel tubes 1 for each animal e 1
8. 10 1 4 TRANSPARENT CYLINDER 11 11 1 11 3 12 13 14 LE119 AIR PUFF ACCESSORY CONNECTING THE ELECTROVALVE TO THE EXPERIMENTATION CAGE COMPONENTS AIR VALVE DISCONNECTION TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Behavioral box and load cell control unit Harvard Apparatus 23 25 27 27 28 28 29 31 32 Panlab M Musics 7 INTRODUCTION The LE 111 unit has 2 functions a Amplify and filter the signal coming from the load cell which is placed in the experimentation cage It displays the animal movement on an activity indicator It also sends the data to the computer for analysis in the program The movements that the animal makes on the grid fear conditioning or the forced convulsions startle reflex are registered by the load cell as mass displacement b Amplify the acoustic signal to stimulate the animal in the experimentation cage This audio amplifier takes the output signal of the LE 118 and sends it to the speaker in the experimentation chamber Figure 6 LE 111 Load Cell Coupler This system features an improvement over conventional systems that use photoelectric cells which give discrete results steps in the movement detection Systems based on load cells give continuous and accurate data With the appropriate response analysis it is even possible to identify different typologies associated with animal behaviour WARNING When using this equi
9. Hardware User s Manual Behavioral box and load cell control unit Startle amp Freezing References E116 76 0280 LE111 76 0281 LE115 76 0328 LE119 76 0286 Version V22 10 2014 Panlab s l u International Calls 34 934 750 697 C Energ a 112 Domestic Call 934 190 709 P a n a b H Harvard 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Apparatus Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab H Medis 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN touched MA AA operative life Waste Electrical and Electronic Equipment Directive WEEE Ww 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failur
10. ROOF CAGE The Soundproof cage is designed to contain the experimentation cages It has additional sound insulation with an internal foam coating Figure 10 Soundproof cage front and side view There is a fan on the left wall to provide ventilation There is a led lamp on the upper part of the rear inner wall with adjustable intensity Figure 11 Fan and Lamp There is a small box with the controls INTENSITY e POWER main switch that turns on fan and light e POWER LED 3mm red led that comes on when power switch is on e INTENSITY knob used to regulate led lamp intensity e CONNECTOR this male jack on the panel is used to connect the external power supply The power supply output must give 12V DC 1A POWER Figure 12 Control box Behavioral box and load cell control unit 15 Panlab J A Harvard A Apparatus 9 WORKING WITH THE EQUIPMENT 9 1 EQUIPMENT CONNECTION The following figure features an example of control over 2 cages The LE 118 can control up to 8 cages Figure 13 Example of two cages connections Behavioral box and load cell control unit 16 Harvard Pa n la b A Apparatus The necessary connections are detailed in the following table o FROM TO ABLE 10 Pressured air system Valve Pipe u Vave Cage Pipe All of these connections are necessary for each cage Pneumatic circuit connections Behavioral box an
11. Racord to pass the wall e 1Racord to extend the connection Option B e 2plastic tubes am long e 1Allen key number 4 11 3 AIR VALVE DISCONNECTION To disconnect the air valve from the stainless steel tube Option A or from the plastic pipe Option B proceed as follows Press the black plastic ring inside 1 while it is pressed remove the valve from the tube pipe A p E P Flow sense Behavioral box and load cell control unit 28 Panlab H Medis 12 IROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION e Ensure that the voltage of mains is the same as that selected in the The equipment does not start up fuse holder Check the condition of the fuses Check connections see Figure 13 Check that AIR led lights on the front panel of LE118 if not can be a problem in the drivers or the USB Air stimulus does not work cable connection Check that the source of compressed air supplies air to the electro valve Check connections see Figure 13 Check that SHOCK led lights on the front panel of LE118 if not can be a problem in the drivers or the USB cable connection Check that SHOCK CALIBRATION switch in the Shocker is in the SHOCK position Check that the INTENSITY knob in the Shocker is set to a value higher than omA Check that the grid is clean urine and excrements can conduct current The animal does not receive electrical shock Check connect
12. d load cell control unit 17 9 2 ADJUST TRANSDUCER GAIN To adjust transducer gain proceed as follows 1 Takethe animal and the restrainer from the cage 2 Set the USE OFF selector in USE position 3 Setthe AC DC selector in DC position 4 Adjust the GAIN to 200 5 Adjust the activity indicator to zero with the help of ZERO knob 6 Set GAIN to 5000 and Gain readjust the activity 500 1999 2000 indicator to zero with the 499 help of ZERO knob 7 Put a 20gr weight on the grid The activity indicator will show a value This value can be increased decreased with the Adjust Load Cell potentiometer on the rear panel It must be adjusted so that with the weight of 20gr it displays a value of 1 0 Behavioral box and load cell control unit 5000 Panlab H Msi DC AC Gain 500 od 2000 200 5000 Load Cell O 18 Panlab H Adae The value shown by the activity indicator and the voltage in the BNC connector labelled OUTPUT are respectively with 20gr the following 4oomV 200mV 9 3 PREPARING THE EXPERIMENT Once all the connections are completed proceed as follows to prepare the experiment Use 1 Setthe USE OFF selector in the OFF position Off 2 Setthe AC DC selector in the AC position AC AC Filter Hz 0 5 0 2 1 3 Setthe FILTER selector to 0 5Hz Gain soo 1900 4 Adjust the GAIN to 1000 200 2000 5000 5 Place the animal on the cage Be
13. e holder door 3 Extract fuse holder using the screwdriver Ee EM Gr LIT ric Fg sate EM Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety reasons never open the equipment The power waRNING SUPpply has dangerous voltage levels Behavioral box and load cell control unit 7 Panlab H Medis 6 TABLE OF CONTENTS 1 SYMBOLS TABLE 2 2 GOOD LABORATORY PRACTICE 2 3 UNPACKING AND EQUIPMENT INSTALATION 3 4 EQUIPMENT INSTALLATION 5 5 MAINTENANCE 6 6 TABLE OF CONTENTS 8 7 INTRODUCTION 10 8 EQUIPMENT DESCRIPTION 11 8 1 LE 111 FRONT PANEL 11 8 2 LE 111 REAR PANEL 13 8 3 EXPERIMENTATION CAGE 14 8 4 SOUNDPROOF CAGE 15 9 WORKING WITH THE EQUIPMENT 16 9 1 EQUIPMENT CONNECTION 16 9 2 ADJUST TRANSDUCER GAIN 18 9 3 PREPARING THE EXPERIMENT 19 9 4 GRID CLEANING 20 9 5 TRAY CLEANING 20 9 6 TRANSPARENT DOOR CLEANING 20 9 7 WALLS CLEANING 21 10 HOW TO INSTALL THE LE 115 CONTEXTUAL KIT 22 10 1 COMPONENTS 22 10 1 1 WHITE UNDER GRID BASE 22 10 1 2 SMOOTH FLOOR 22 Behavioral box and load cell control unit 8 Panlab H 10 1 3 WHITE WALLS
14. es which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Behavioral box and load cell control unit gt Panlab H Medis 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton wit
15. h its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G AN Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits AN For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category II over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Behavioral box and load cell control unit 3 Panlab H Medis PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to
16. havioral box and load cell control unit 19 Panlab m Medis Use 6 Setthe USE OFF selector in USE position Off 7 Look the movement of the activity indicator if the signal is low then you must increase the GAIN 9 4 GRID CLEANING When there is dirt in the grid the electrical shock flows through it This will produce erroneous data during the experiment because the animal behaviour to the shock negative reinforcement will not be correct it will not receive punishment In order to clean the grid you can use water and soap taking care to dry it afterwards The grid set can be removed from the tray in order to clean it Be sure to dry well the DBo connector otherwise contacts will rust with time Special care must be taken in cleaning the plastic between grids because urine is a good electrical conductor and current flows through it 9 5 TRAY CLEANING The tray that contains the grid collects faeces and urine it should be cleaned after each experiment In order to clean it the tray should be removed from the cage and washed with a soapy solution finally it should be dried 9 6 TRANSPARENT DOOR CLEANING WARNING In order to clean transparent door never use neither alcohol nor N alcoholic derived products otherwise stripes will appear in the transparent plastic To clean the door you can use a slightly wet cloth and afterwards dry it with a dry cloth If it s too dirty it can be cleaned with a cloth with a soapy solution
17. hocks are delivered Behavioral box and load cell control unit 22 Panlab HA rever during this phase of the experiment grid animal contact is not necessary The smooth floor does not impair the sensitivity of the system in detecting animal movements 1 Cover the grid with the aluminium floor 2 Place the set back into the cage Figure 15 Smooth floor 10 1 3 WHITE WALLS The white walls change the visual perception of the subject with respect to the colour of the box Three walls are provided including one for the left side featuring the light Figure 16 White walls 1 Open the door of the box and partially unscrew the 4 white screws of the top cover of the box Figure 17 White screws Behavioral box and load cell control unit 23 Panlab He cubic 2 Maintain the cover slightly raised and insert the bent part of the white wall in the gap between the cover and the top part of the original black wall IR p I L Y 3 3 F vr le y a M x M i LA UN E n e y P EE Ya J et te a NT run t EN kA N poft oe er cp Figure 18 White wall placement 3 Repeat the same process for all 3 walls left right and back walls Figure 19 White walls placement 4 Screw the 4 white screws of the top cover back down to keep the walls firmly fixed in place 5 Once fixed it is important to ensure that the bottom parts of the 3 walls are NOT touching the to
18. ions see Figure 13 Check that LIGHT led lights on the front panel of LE118 if not can bea problem in the drivers or the USB cable connection Light stimulus does not turn on Check that the bulb is not burned out Werno edo rorcound e Check connections see Figure 13 Behavioral box and load cell control unit 29 Panlab H Medis Check that NOISE led lights on the front panel of LE118 if not can be a problem in the drivers or the USB cable connection Check in the program that the duration and selected Sound level are enough so that the white noise is audible Check connections see Figure 13 Check that SOUND led lights on the front panel of LE118 if not can be a problem in the drivers or the USB cable connection Check in the program that the duration and selected Sound level are enough so that the tone is audible The tone does not sound Below 100dB the needle will not move being the minimum Check connections see Figure 13 Check with the help of PC speakers that the sound card of the PC runs The VU meter needle does not move Ensure that the grid and tray are correctly positioned Remove the tray and check in the four tray supports of the platform The computer does not detect the when pressing on them they are animal s movements not blocked and move Increase the gain with the GAIN knob on the front panel of the E113 Behavioral box and load cell control unit 30 Panlab H
19. lage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt PE O Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni
20. lity for improper use of the equipment or the consequences of use other than that for which it has been designed Behavioral box and load cell control unit 5 Panlab H ae 5 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment or if the equipment voltage setting is incorrect check fuses according to the following procedure 1 Remove power cord from the power inlet Behavioral box and load cell control unit 6 Harvard Apparatus Panlab H 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fus
21. mbiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de pue usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente I lt Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyc
22. p part of the floor grid tray as this may affect system sensitivity Behavioral box and load cell control unit 24 Panlab HA teer 10 1 4 IRANSPARENT CYLINDER The transparent cylinder is used to change the spatial perception of the internal space inside the box The cylinder is provided with an open top for the light stimulus placed on the left wall and 3 small holes on its bottom to ensure fixation of the whole cylinder in the box without any contact with the floor The cylinder can be fixed in the box with the original black walls or in the box in which the contextual white walls have been placed this is why the cylinder is transparent so that the subject can perceive the new colour of the box when visual and spatial contexts are both changed Figure 20 Transparent cylinder 1 The cylinder is provided with 3 small screws 2 Screw the screws into the 3 small holes found on the bottom part of the 3 original black walls of the box Figure 21 Screws Behavioral box and load cell control unit 25 Panlab HA terere 3 Insert the cylinder by the front door pushing it laterally and matching its 3 holes with the 3 screws standing out from the walls Figure 22 Placement of the transparent cylinder 4 Once fixed it is important to ensure that the bottom part of the cylinder is NOT touching the top floor grid tray as this may affect system sensitivity NOTE in standard boxes the back wall is black and not
23. pment hearing protections should be used as to avoid taking damage due to the high acoustic levels the sound can reach up to 120 dB Behavioral box and load cell control unit 10 Panlab H Medis 8 EQUIPMENT DESCRIPTION 8 1 LE 111 FRONT PANEL ON AC DC FILTER GAIN OUTPUT CELL COUPLER LE 7177 Cell Running AC Filter Hz 0 2 0 P 1 Output Panlab HARVARD APPARATUS Use Off ZERO CELL RUNNING Figure 7 LE 111 Front Panel e ON Green coloured led that remains on when the main switch is on e USE OFF This selector enables disables the load cell signal It facilitates quick deactivation of the cage signal and measurements It is useful when placing removing an animal from the cage and when a register is begun concluded This selector must be in the USE position when an experiment is being conducted e AC DC This selector acts in the coupling of the signal DC The unit measures the absolute value of the transducer signal continuous and alternate values This mode is useful for Adjusting and equalizing the sensibility in several control units When equivalence between mass and the electrical signal that the equipment gives is desired AC The unit only measures the alternate changes in the transducer signal by removing the continuous part of the signal This is the normal working mode as only the changes of mass are needed e ZERO This potentiometer only acts in DC It can be used to adjust the output
24. s potentiometer can be used to Give the total gain of the unit an intermediate value with respect to the scale in the GAIN selector values for example 3000 Couple several LE 111 modules with their load cells so that they give the same signal when the same weight is applied e ADJUST AMPLITUDE Fine adjustment of the amplitude of the audio signal it changes the volume This potentiometer can be used to Adjust the volume in the cage to a desired value for example 8odB Couple several LE 111 with their speakers to get the same acoustic volume e SHOCKER Controls the LE 100 26 shocker through the External Time located on its rear panel e AIR Opens an electro valve to supply an air stimulus to the animal An external source of pressured air must be used as a compressor e CHAMBER Controls the chamber speaker and light e MAIN SWITCH The main switch the fuse holder and the power inlet Behavioral box and load cell control unit 13 Panlab H Adice 8 3 EXPERIMENTATION CAGE LIGHT SPEAKER BOX DOOR PLATFORM GRID Figure 9 Cage The cage has the speaker box on its top There are 2 connectors one for interfacing with the LE 111 and the other for the light There is a load cell under the platform to detect animal movement in response to the pulse The grid has a delta 9 connector to administer shock when necessary Behavioral box and load cell control unit 14 Jon mzzon Panlab HA teer 8 4 SOUNDP
25. t 10096 to 3396 respect max LOAD CELL Excitation voltage Input impedance Output impedance Sensibility Total error Thermal zero shift 12V 409 ohm 350 9 ohm 1 95 mV V 47uUV V 0 050 C N 10 C Behavioral box and load cell control unit 32 Panlab H Harvard Apparatus Thermal sens Shift lt 0 120 C N 10 C SHOCKER OUTPUT TTL output level low o 5Vmax high 2 4Vmin LIGHT OUTPUT TTL output level low o 5Vmax high 2 4Vmin CONNECTOR CONTROL 9 pins Pin DPNWNOW 00 Function output signal analogic gnd TTL input light TTL input shock gnd digital load cell input load cell input CONNECTOR CHAMBER 4 pins Pin Function B loudspeaker D loudspeaker A TTL output light C GND digital SPEAKER Diameter 165mm Magnet 170g Impedance 40 Music maximum power 170 W Nominal power 35 W Frequency range 200Hz 10kHz DIMENSIONS Width x Height x Depth Weight 232 mm x 124 mm x 297 mm 5 31 kg Behavioral box and load cell control unit 33 Panlab H Medis DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el
26. to o when there is no signal it compensates for the offset of the transducer and the weight of the grid Behavioral box and load cell control unit 11 Panlab H pod ds e ACFILTER HZ This control only works in AC mode It selects the cut frequency of the low pass filter The value displayed is the value at which the signal will have an attenuation of 3 dB At 0 2 Hz the unit will take slower movements than at 1 Hz e GAIN This is the factor by which the signal of the load cell is amplified to pass to the output More gain is needed for mice than for rats e CELL RUNNING This activity indicator shows the absolute value of the output signal n AC It displays the movement In DC It displays the mass e OUTPUT The output signal is the load cell multiplied by the gain and filtered if it isin AC mode Behavioral box and load cell control unit 12 Panlab H judi 8 2 LE 111 REAR PANEL LOAD CELL PLATFORM LOAD CELL AMPLITUDE Load Cell Platform Control Air Shocker Chamber Aud ES e Q 9 O59 Made in Spain 110 220 V C AC 47 63 Hz 80W CONTROL AIR SHOCKER CHAMBER MAIN SWITCH Figure 8 LE 111 Rear Panel e LOAD CELL PLATFORM This connector takes the signal coming from the load cell placed under the platform of the experimentation cage e CONTROL This connector joins the LE 111 with the LE 118 for communication e ADJUST LOAD CELL Fine adjustment of the GAIN The gain can be increased decreased Thi
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