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1507340_ver2.4 Wellwash 4 Mk 2 User Manual

1. Further instrument system developments desired Further applications desired Where did you first learn about the product Would you like to receive information about other Thermo Fisher Scientific products Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 66 Appendix D Addresses Appendix D Addresses For the latest information on products and services visit our worldwide web sites on the Internet at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific Oy P O Box 100 FI 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 67 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 68 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 69 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 70
2. a mn B mg n o w in Y E 5 5 Ed 6 0 Combiplate 4 spare fuses see Section 7 1 10 Fuse replacement 2 Allen keys see Section 5 3 Installation procedure step 1 2 spanners see Section 5 3 2 Wash head height De clogging tool see Section 7 1 9 Unclogging tips 6 spare O rings for the bottle caps 4 spare O rings see Section 5 3 Installation procedure step 5 Poe a Fig 5 1 Accessories included with the instrument Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 14 Chapter 5 Installation Waste liquid spare tube Reagent liquid spare tube Pinch valve spare tubes item 3 in Fig 5 3 see Section 7 1 8 Pinch valve Wash arm assembly spare tube item 2 in Fig 5 3 Wash arm assembly spare tube item 4 in Fig 5 3 Wash arm assembly spare tube item 1 in Fig 5 3 aOuKRWN Fig 5 2 Spare tubing Fig 5 3 Wash arm assembly tubing 5 2 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the car
3. X 0 5 MIN PAUSE VOLUME X50 pl Fig 6 3 Programmable switch card 6 5 1 Two way switch functions Thermo wzuwWAsH4 MK2 El A Bway wav OFF f STEPOVR A ato OD WASHHI waenee rf ory F1 orr FINALX1 e Fwer Fory E STRIP F FINALX2 Switch settings SINGLE Make sure that this switch is always set to single wash head 8 WAY 12 WAY Use with an 8 way wash head Use with a 12 way wash head PLATE STRIP All selected rows are washed once the instrument waits during the PAUSE setting and the following wash cycle is started Each row is washed the set number of times WASHES The PAUSE setting is between each row STEPOFF The plate carrier will only pass over the selected rows With this selection the plate processing times are kept to a minimum STEPOVR The plate carrier will pass over all the rows not washing the rows not selected With this selection the time used for each plate remains constant WASHLO The wash head fills the well while rising from the bottom of the well WASHHI The wash head fills the well from the raised position WET The plate will remain filled after washing Use this setting with soak period DRY The plate will be aspirated after washing F WET The plate will remain filled after the final wash F DRY The plate will be aspirated after the final wash Rev 2 4 Cat no 1507340 32
4. 90 max non condensing Pressure Max pressure on vessel 7 psi 0 5 bar self limiting Plate type 96 well plate Containers Material HDPE high density polyethylene Wash bottle capacity 2 liters Waste bottle capacity 2 liters Additional wash bottle capacity 2 liters Fluid consumption Wash fluid consumption approx 150 ml complete microplate Priming consumption approx 15 20 ml Residual aspiration volume lt 5 ul per well using 0 02 Tween solution Dispensing precision 5 using 0 02 Tween solution Wash heads 8 way and 12 way Material POM polyoxymethylene polyacetals Wash program cards 1 4 washes Programmable card Volume 0 750 ul Washes 1 15 Soak time 0 10 minutes Pause 0 4 minutes Wash time 120 s 3 x 350 ul with 12 way wash head 165 s 3 x 350 pl with 8 way wash head Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 55 Chapter 9 Technical Specifications 9 2 Safety specifications Safety performance EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 9 3 In conformity with the requirements Wellwash 4 Mk 2 bears the following markings Type 006 100 120 Vac 200 240 Vac 50 60 Hz 300 VA CE mark Wellwash 4 Mk 2 conforms to the following requirements 2006 95 EC Low Vol
5. F1 OFF FINAL X1 and F2 OFF FINAL X2 Table 6 2 Number of washes after a soak period Settings Number of washes F1 OFF F2 OFF 0 FINAL X1 F2 OFF 1 F1 OFF FINAL X2 2 FINAL X1 FINAL X2 3 PLATE STRIP and WET DRY Table 6 3 Aspiration after the final wash Chapter 6 Operation Settings PLATE WET No aspiration wells filled PLATE DRY All rows aspirated after the final wash STRIP WET No aspiration wells filled STRIP DRY Each row aspirated after its final wash 6 5 2 Rotary switch functions SOAK Set the soak period Table 6 4 which commences after all the presoak wash routines WASHES are complete Make sure you have selected WET Table 6 4 Soak period in minutes Setting 0 1 2 3 5 6 7 Soak time minutes 0 05 10 11 5 20 25 30 3 5 Setting 9 A C D E F Soak time minutes 4 0 45 5 0 16 0 7 0 8 0 9 0 10 0 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 33 Chapter 6 Operation PAUSE Set the pause between each wash cycle Table 6 5 During the strip wash mode the pause is between each row During the plate wash mode the pause is between each pass over the plate Note The times currently available are subject to approval and may be altered 1 to suit individual customer requirements Table 6 5 Pause in seconds between each washing cycle Setting 0 1 2 3 4 5 6 7 Strip wash mode second
6. Fig 5 9 item 2 4 Connect the liquid level sensor cord from the liquid trap bottle to the waste bottle Make sure the black connector is firmly in place Fig 5 9 item 1 Caution The liquid level sensor does not function if the connector is not properly inserted the black connector has to be pushed down all the way The waste bottle may otherwise overfill and cause the liquid trap bottle to start filling If at that point the liquid trap bottle is not emptied the overflowing waste liquid can enter the pump unit and damage it 1 2 3 Liquid level sensor connector Vacuum feed tube Liquid trap bottle cover Fig 5 9 Liquid level sensor system 5 Check that the tubes are properly connected to the pinch valve under the wash arm assembly on the washer unit Fig 4 1 item 14 The thin tube going through the valve should not be pinched by the edges of the valve Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 20 Chapter 5 Installation 6 Remove the three 3 black plastic caps from the hose connectors and the pump control at the rear of the washer unit Fig 5 10 110 420 VAC __ 60 Hz 24 VA Fig 5 10 Removing the plastic caps 7 Slide the two units together until the locating dowel and electrical connectors are firmly engaged Fig 5 11 Silicone tube waste blue color code Manifold pipes PRESSURE for reagent tube yellow VACUUM for waste tube blue Locating pin
7. 2 Prime the unit with distilled water from the wash bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 46 17 18 Chapter 7 Maintenance Lift the wash head and fill the prime tray with glutaraldehyde solution Aspirate the prime tray using the wash head and then reprime the prime tray with distilled water Remove the plate carrier and the wash head and place into a glutaraldehyde solution for 24 hours Empty the fluid containers Switch OFF the power and disconnect the mains supply cable Decontaminate the outside of the unit using a wad of cotton wool soaked in 70 alcohol Place the entire instrument into a large plastic bag Ensure that the reagent containers are disconnected and open Place a wad of cotton wool soaked in 10 formaldehyde solution into the bag Ensure that the wad does not make contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag Clean the instrument using a mild detergent Remove any stains using 70 alcohol Take the plate carrier and the wash head out of the glutaraldehyde solution and rinse with distilled water Reposition them on the unit Flush the fluid path of the instrument with deionized distilled aqua using the priming procedure The wash heads can be sterilized in 70 ethanol Also wash and decontaminate the wash rinse and waste bottl
8. a and friction bush b Silicone tube wash yellow color code Signal connectors with text PUMP CONTROL Silicone tube pressure black color code Pump pressure adjustment screw do not touch for authorized service personnel only 1 Ze 3 4 5 6 7 Fig 5 11 Connection of pump and washer units Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 21 Chapter 5 Installation 8 Open the wash bottle Fig 4 1 item 4 by rotating the cap ring while the white plug remains stationary Lift the cap ring and open the plug Fill the wash bottle up to the shoulder with wash reagent and reconnect ensuring that the plug mates correctly with the bottle insert Fig 5 12 and that the cap ring is tightly screwed down Fig 5 12 Opening and closing the bottle 9 Tighten the cap on the waste bottle 10 Lift the wash head and slide the plate carrier onto the stainless steel slideway so that the reservoir is under the wash head Fig 5 13 1 Wash head 2 Priming reservoir 3 Plate carrier Fig 5 13 Correct position of the plate carrier Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 22 Chapter 5 Installation 5 3 2 Wash head height To accommodate the differences in size and volume capacity of manufacturers microplates the height of the wash head is adjustable This means that a positive meniscus can always be obtained without an unnecessary waste of wash r
9. 9 3291 0415 www thermo com Rev 2 4 Cat no 1507340 Table of Contents Table of Contents 0 N O Ul Rh tU MN so 10 11 12 13 Appendix A Appendix B Appendix C Appendix D Safety Symbols and Markings ceeeeeeeeeee eese eene nennen hehehe enne 7 About the User Manual cceeeeseeeeseeeeeee eene e eene senes hes n tese s rete n renun 8 Introduction to the Wellwash 4 MK 2 eeeeeeeeee eee eene eene enne hehehe 9 Functional Description eire eee en oe re au eene tenen ro a eroe ae 11 Insel 13 Operatiom TH 26 MAINteNANCe ote coe oe dueno encia a E oe eine om E n 36 Tro bleshooting 21 cec oreet ee ere erre eren no ben re nre npe be apne sre abren eeupa 49 Technical Specificatioris 0 2 oret o rer eter a eee S Uer eoo e eer esses Uer s eues 55 Ordering Information ceeeeeeeeeeee eese eee ee hene hee hene rhet eher 57 Warranty Certificate icons cian 58 iiy ee 59 Glossary and Abbreviations eeeeeeeeeeeee eese eene eene hene eher enne 63 Wellwash 4 Mk 2 Quick Reference Guide eeeeeeeeee eee eee eene nennen 64 Certificate of Decontamination ccscsccsccsccsccccceccesccscescescescescesceseesees 65 Wellwash 4 Mk 2 Feedback FOrim ssscsscsscsccsccsccsccsccsccscccscsscescessessessess 66 Addresses 2 ip ERI TUER EE E PORRO IROI PE Qoa cabo
10. The wash head will then lower itself to empty the prime reservoir The PRIME indicator will turn off when the priming is complete If no further key entry is made within ten 10 seconds of priming the pump will automatically switch OFF The instrument will then enter its idle state waiting for instructions from the keypad and program card 7 Place a microplate onto the plate carrier When using a 12 way wash head the well A1 of the microplate should be positioned in the top left hand corner When using an 8 way wash head the well A1 should be positioned in the top right hand corner See Fig 5 16 Fig 5 16 Placing the microplate with 12 way and 8 way wash head 8 Select the row number of the last row to be washed on the keypad e g when button 4 is pressed rows 1 to 4 will be washed Note When the 12 way program card is installed buttons 9 to 12 are inoperative 9 Press the START button to set the washing cycle into operation The plate carrier will be drawn into the washer filling and aspirating each row according to the sequence selected on the program card At the end of a sequence rows will be left substantially dry and the plate carrier will stop with the priming reservoir under the wash head 10 The instrument is performing properly if the wells are dry If they are not check that both bottle caps are firmly closed Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 25 Chapter 6 Ope
11. clogging of the wash aspirate tips To avoid this the following procedures should be followed 6 7 1 Daily shutdown procedure When the last wash cycle of the day is complete switch the power OFF through the switch at the rear of the pump unit Fill the priming reservoir of the plate carrier with distilled water and lower the wash head tips into the priming reservoir Do not detach the tubes unless you first open the wash bottle cap slightly to release the pressure Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled aqua a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the instrument disinfect with 70 alcohol or other disinfectant See the Wellwash 4 Mk 2 User Manual Section 7 3 Last of all put the dust cover on 6 7 2 Long period shutdown If the washer is not likely to be used for a long period you should flush the system through with distilled water prior to carrying out the DAILY shutdown procedure Empty and clean the waste bottle and check that the liquid trap is dry When the washer is to be used after a prolonged shutdown you should prime the system Section 6 3 at least four 4 times prior to carrying out any wash function 6 8 Emergency situations In case there is any abnormal situation during operation such as fluids spilling inside the instrument follow these steps 1 Switch OFF the instrument 2 Unplu
12. eene nhe enne nhe een 15 Fig 5 4 Location of the frequency selector switch and the transit screws 17 Fig 5 5 Pinch valve holder cessssseeesseseeeee eene eee nhe hehe nhe 18 Fig 5 6 Installing the O rings to the wash head holders e eeeees 18 Fig 5 7 Sliding the wash head between the holders ceeeeeeeeeeee ee 19 Fig 5 8 Pump unit with tubes installed cseeesseseeeee eee nnne 19 Fig 5 9 Liquid level sensor system ccsesesseeeseeeeee eene ehe enhn enhn 20 Fig 5 10 Removing the plastic caps cssssseesseeeeeeeeee nene ehe enhn 21 Fig 5 11 Connection of pump and washer units c eesesesseeeeee eene 21 Fig 5 12 Opening and closing the bottle cccc cece cece cence cence eeneeeeeeceeeneeeeees 22 Fig 5 13 Correct position of the plate carrier ccc ccece cece eee ceeneceeeneeeeneeeeees 22 Fig 5 14 Loosening the locknut with spanners ceesseeeeeee eene nhe 23 Fig 5 15 Changing the wash head cssessseeeeeeeeee eene ehe ehh nhe 24 Fig 5 16 Placing the microplate with 12 way and 8 way wash head 25 Fig 6 1 Releasing the pressure from the wash bottle ceeeeeeeee eee 30 Fig 6 2 Preprogrammed card three washes with a 12 way wash head 31 Fig 6 3 Programmable switch card ccsssseessseeeeseee e eee e
13. ence e ence cence eee ehe hee enn 33 Table 6 5 Pause in seconds between each washing cycle e eeeeeeeee esee 34 Table 6 6 Number of washes before soaking ccssseeeseeeeeee eee enne 34 Table 6 7 Washing volume in ul eeesseeeeeeeeeee eene enne e he nhe nhe 34 Table 7 1 Maintenance check liSt 2 ccc sce e scence ence eee e ee enee eee eee ee eee 36 Table 8 1 Troubleshooting list ccc cece cece cence ce enc eee ne eene enne nhe enhn 50 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the Wellwash 4 Mk 2 Power ON C Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use V In Vitro Diagnostic Medical Device WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC r E E Warning markings used in the documentation Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Other mark
14. lowering the domed nut and locking into position on the head lift rod 5 3 2 Tighten cap replace gasket if necessary 8 Leakage between wash cycles Pinch valve not functioning properly Push the pinch valve and pull the tubing through the valve and reform 7 1 5 9 Wash arm assembly not moving as it should Contact authorized service Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 50 Chapter 8 Troubleshooting 10 Plate carrier not moving as it should Obstacle on the plate carrier slideway Keep the slideway clean 11 Wash cycle does not correspond to the program card Program card not inserted all the way Program card is dirty Program card is damaged Push the program card all the way into the slot Clean the program card Replace the program card Contact your local Thermo Fisher Scientific representative 12 Instrument not functioning as it should Press the RESET button If that doesn t help switch the instrument OFF and ON again If the problem persists contact authorized service DO NOT use the instrument if it appears that it does not function properly If you were able to correct the error without having to turn off the instrument you may continue instrument operation by pressing start Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 51 Chapter 8 Troubleshooti
15. microplate It comprises a pump unit and a washer unit Fig 4 1 which are connected by power air and liquid lines The pump unit consists of a double headed vacuum pump with high density polythene reagent and waste bottles and a liquid trap The main power ON OFF switch is at the rear of the pump unit The frequency selector switch is located on the bottom side of the pump model Cat no 5190771 near the transport locking screws The keypad is located on the left hand side of the washer unit and the microprocessor and associated control electronics are housed under the keypad The wash head is located above the plate carrier slideway which can be either 8 or 12 way The plate carrier moves the microplate accurately beneath the wash head and incorporates a priming reservoir at its leading edge The plate carrier is indexed forward by means of a magnetic coupling to the drive system housed inside the washer 1 Pump unit 8 Wash arm assembly 2 Washer unit 9 Wash head and wash head holders 3 Waste bottle 10 Priming reservoir 4 Wash bottle 11 Keypad 5 Bottle caps 12 Plate carrier 6 Liquid level sensor 13 Program card 7 Liquid trap bottle 14 Pinch valve Fig 4 1 Wellwash 4 Mk 2 microplate washer The pump creates pressure to the wash bottle Fig 4 1 item 4 allowing the wash liquid to be dispensed through the pinch valve Fig 4 1 item 14 into the microplate wells and a vacuum to the waste bottle Fig 4 1 item
16. microplates with samples of human or animal origin keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood If the pump fails to start or if the bottle caps are loose an audible warning will be heard and the VAC FAIL indicator will illuminate This condition will clear once the pump has started If the VAC FAIL indicator does not clear after the pump has started the RESET key must be depressed Also check that the waste bottle is not full The priming procedure must be repeated whenever the POWER switch has been engaged or if there has been an interruption of the power supply Make sure that sufficient reagent is present in the wash bottle to complete the required protocol See Fluid consumption in Section 9 1 General specifications If you use the programmable switch card ensure that the 8 WAY 12 WAY switch is in the correct position for either the 8 way or the 12 way wash head If removable well microplates are being used make sure that there are no missing wells in the rows to be washed and that all the wells are at the same level If you press the RESET button on the keypad at any stage this will stop the immediate washing sequence and reset the system to its pre programmed state DO NOT smoke eat or drink while using the Wellwash 4 Mk 2 Wash your hands thoroughly after handling test plates Observe normal laboratory procedures for handling pote
17. tarews ens 11 Number Of washes fa A I RR ET RR RR ARP EET RE RE Lee EE T uir peus 33 O OPperatina COTOS vii E A ATA IA IIA ARSUE XE 15 Operational check catas IS EA ETS Aisa 23 Ordering information cto TT 56 Overfillind Wells iii c e 49 P hdc Es 47 for service RE RE A ORE RE ERR AREE A sls ERR AREE EIA oe a 46 he C 13 Pause between washes oooococcconcconcconononn eens eens eens eens sees sees theres ern ere ere rne ern eren rne 33 PINCH valve i a y inr Y EROR SRe NY YSRASe RESTER RASEN E AE Gbu0 B60 cdi dda d does daset bee 10 19 28 holder wasn vs DEEP 17 unpinching c 6 ici e rc IRR een cee ec ee 41 Plate eto etre or e rto oe d ve x rit oin eve es watts vae tus ciet vat vec nee Urat Usi se 9 34 Claim PEE 34 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 12 Index Plate carrier aoi coo rox depu ia 10 26 28 POU moving 2 shiacadn E NN 49 SPIWAGES E DERE 36 Plate Wash mode lA A toe ce n 31 Power cable 4 er re LR ee eas 23 Power indicator ii eb ley eR D e Ele Dee Re E EEKE REEERE EREEREER EEEREN 11 POWet SWItCh uoce eer enr ear boy is 10 11 Prime indicator ice ee n a ead ee e DR eee 11 Ma RS Se etc ei ea Ss ee a he ee ee Se eee ca he ee 24 26 Prime PUSH bUttOD este eR ER REEF ERE RA AAA 11 Priming vasa aah E vated aisle vate RR waa viele vated side seta ENSE ERR SERE HVEFRAMEA E ERES 24 28 Priming reservon asses AS A ASAS RC SPAERE ER EERERMA dees ERES 10 Program Ca
18. the VAC FAIL indicator does not clear after the pump has started the RESET key must be depressed Check also that the waste bottle is not full Note The priming procedure must be repeated whenever the POWER switch has been operated or if there has been an interruption of the power supply Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 29 Chapter 6 Operation 6 4 Adding wash reagent To add wash reagent first release the pressure from the wash bottle by carefully opening the bottle cap see Fig 6 1 Let the hissing sound end prior to opening the cap all the way Fig 6 1 Releasing the pressure from the wash bottle Also when you are changing the used reagent and you wish to rinse and clean the tubing first release the pressure from the tubing and the wash bottle by slightly opening the wash bottle cap Close the wash bottle tightly Fig 5 12 The waste bottle needs to be emptied when the wash bottle is filled Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 30 6 5 Program selection a EEG Chapter 6 Operation Choose an appropriate card for the wash routine Plug the card into the slot located at the base to the right of the washer unit Note Note 1 Note 2 Note 3 Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction
19. the instrument if any biohazardous material has been spilled on it or when shipping it to service See Section 7 3 Perform the operational check regularly See Section 5 4 Arrange for the yearly servicing of the instrument b Section 7 1 1 above 7 1 3 General Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied For reliable daily operation keep the instrument free of dust and spills from liquids It is also advisable to cover the instrument with the dust cover supplied when not in use In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 36 AN A Chapter 7 Maintenance It is recommended that you clean the case of the instrument periodically to maintain its good appearance A soft cloth dampened in a warm mild detergent solution will be sufficient Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer DO NOT expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Clean the keypad with a mild laboratory detergent Plastic
20. washer and pump units into plastic Drop them into their own places onto the bottom part of the package shock absorber 3 Put the top part of the package shock absorber into the box Ensure correct positioning and the correct location for the manifold support piece 4 Put the bottles into Minigrip plastic bags and drop them into their own locations in two corners of the box 5 Put the two small carton boxes onto the top part of the package shock absorber DO NOT place the small boxes on top of each other but place them instead into their own specific locations 6 Put the rest of the items user manual labels notice packing list etc into a Minigrip plastic bag and place it into its own position on the top part of the package shock absorber 7 Close and seal the box 7 5 Disposal of the instrument AR Warning Decontaminate the instrument prior to disposal See Section 7 3 and Appendix B on decontamination Also wash and decontaminate the bottles prior to disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Dir
21. 3 Checking delivery for completeness cesses 14 Checking for damage during transport eese 15 Environmental requirements essssssesoossssssososssssssesoessssssooosssssssoossses 16 Thines to avoid ore ERR REI ERE EE ERU TH nes 16 Technical prerequisites i eee rr Ere rer rre pd a Rr e EE e nr ERES ee pS de 16 Installation procedure e rov os saccades dis ash 17 How to install the pump unit and washer unit connections cee eee eee eee eees 19 Wash head heiglit a iio A RE ERROR vat ad Nee IA ia RENT PET 23 Mains supply cable 4 24 eire ce debe cen eee tre e esc dee c ae eo dp e o eese eso aded 24 Changing wash heads 2 cere dete Ses ee dane tied even dune ede ad Dev aca dane 24 Operational Check ii eee oae e eer nn eye ERE e sg XN NE 64 neveu e bees ubera es 24 ergo mS 26 Operational check Einicio E E er ERN FERE RS SPAIN T RV NER ies 26 Washing a microplate etre et eh eee It eo eee ee e pU y o de pr CR pU RE Ede vea e Ed evs 27 Priming procedure o rex te ey yt eR Ue REED RE RERO I E EUER I E UUE UE E ERU E ERES 29 Adding wash reagent 2 3 cis cce terret Rr b ta ee bettneed beedeewenestaced need 30 Program selection p 31 Two way switch functions ccc cece cece cece neces eee ehe eren sns eren nnn 32 Rotary switch functions c cece ee ec eee ee cece eee eene eene ehh etes erase nnn 33 cadant IEEE 35 Shutd
22. 3 to aspirate the used wash liquid into the waste bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 11 Chapter 4 Functional Description 4 1 Controls and indicators Here is a description of the controls and indicators on the Wellwash 4 Mk 2 POWER ON OFF switch This two position switch at the rear of the pump unit controls the electrical power to both the pump and washer units PRIME push button PRIME A momentary action switch that starts the PRIME function An audible tone will sound when pressed to confirm operation O T PRIME indicator CZ e This yellow LED light emitting diode located to the right of the PRIME button flashes to indicate that the PRIME function is required and when continuously 3 ON indicates that the washer is primed and ready for use The indicator will turn off after a while but the instrument remains ready for use 4 e ROW SELECT 1 12 push buttons 5 e These are momentary action switches that select the last row to be washed in any chosen program sequence An audible tone will sound when pressed to 6 e confirm operation J e ROW SELECT indicators These yellow LEDs located to the right of the ROW SELECT push buttons when 8 illuminated indicate the rows selected for washing C9 e START push button This momentary action switch initiates the wash cycle An audible tone will 10 o sound when pressed to confirm oper
23. 3 3 Mains supply cable connection Never use a mains supply cable cord other than the Thermo f Warning Never operate your instrument from a power outlet that has no ground Scientific mains supply cable designed for your region 1 Ensure that the mains switch at the rear of the pump unit is in the OFF position 2 Connect the mains supply cable cord to the mains input socket at the rear of the pump unit If you need to use any other type of mains supply cable than supplied use only cables certified by the local authorities 3 Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded earthed 5 3 4 Changing wash heads You can change the wash head to either 8 way or 12 way Lift the head assembly Fig 4 1 item 8 grasp the wash head Fig 4 1 item 9 at both ends and slide it down in the direction of the tips until it clears the end caps Check that the two O rings in each end cap are in position and undamaged To install the alternative wash head slide it upwards onto the slots making sure that it is fully engaged Fig 5 7 Fig 5 15 Changing the wash head Note The wash heads will only fit into the end slots when the raised flange on i the wash head is facing the front Remember to check the program card accordingly 8 way or 12 way 5 4 Operational check The following procedure should be completed to confirm the correct functioning of the washer prio
24. Ase vated MIR bane Aue vate cviee vans age EABR RA sans Age vatadeiee seus E ERR RR sais ERA exes 12 check e ERE 12 MOI RR ORE ER REPRE AE AAA EPRO ER usta REA AA ER RE oe bas 15 Installing liquid level sensor 2 3m e acia 19 pump and washer units tOQether cceecce sec e nec e nee e nee nee eene ehe ehe ernst ehe ere enne 20 Instrument decontamiNatiON tec 45 f nctional descriptlon 5 eere tee petere ER etr Er ERE TER ELT ER ERE ES UE EE ERES CEN EE T EEUU ES anes 10 Ive deter dass 14 MATISSE E aE Tee 55 safety symbols and markings aesa E eene ehe ehe ehe etes ehe eese te reser 7 npacking isses Tee A years paral DRM RM VITA DDR UU TD TOL PI DR V nea TUE 12 K MLB 10 11 CLEANING ii A A EA AAA E 36 L ETRE IMEEM 10 installation 2 tereti RR eR ans aida 19 Bici 10 Liquid trap DOttle culta 10 37 overfilling precautions cse creer ss 19 M Mains supply cable 3 P RE MERGE doy eid VERRE EUR EUM VRINS NS ERE EUN VR ee 23 DARE lite 23 Maintenance odere de eee eee eee ese st pee E een e e a NN REA DOE E e i nie Mei Dalias 35 check list Ee ERE A EE RM ER 2 Ee 35 Manifold pipes x reo reo oe yx saves Vaya oo wee b Love vae e LB yen aUe ya dared Eo a ERE ebd 20 Microplate NANO iii 27 size differences 2 oe ee e eR ER ERI E E EU EUR ERI E ee UE E ER EE ER Le EUN EE I ETE EE 22 N WM EET ICI LUI bees Soambeegeoeed eb
25. EN 0 c E 27 Control panel reese sensora eet ret ete pen epu r eni o id clean das 11 Countdown sequence of row iNdicators cece cee ee cece eee eee e eee eee eee eeeeeeeeeeeeeeeeeeeeeeeeenes 23 D Decontaiiriatiorn iis ecce rere cs deeds PARERE AREE ERAN KR ERE CAR TERRE ARES ARANA AMARA SARA 45 Contificat OF epe E 64 POCAS er KT m 45 DIMENSIONS 2 c 54 PIU CUA eMe 55 DISIMFECLAINES cisco ste antes EmT 45 DISINFECTION PFOCCUUFC RP 34 DISPENSER Mei 33 DISPENSING PRECISION ss iis UEM 54 Disposal iia 2T Sis ard di ala Se adie n 47 materials esee sce AAA A a 45 E Environmental conditions cse esed tenu tea dese tese tore esu te re Devi esie eia dese suas Resa gea eaae des eres 54 F Feedback To TREE ETE TEE ULT 65 FREQUENCY selector vss ena 10 16 dU O cadmas tnannaesdnasee 54 HOPLAGINS EP 43 H Hose CONNCCUOIS ies saad cand vend vans ARAN AREA seat saad sxe sass sand sins dean deans ARAS sexe saad ARAS 20 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 12 Index How to disinfect the instrument eessen e AEA REEE EAEE ERARE EAEE AN 34 l Idle state ti e eie lia 24 30 IndiCators zio secs cuits vis HR ERR MARRE NR seus dvids EAR NER PRSE NAR sass NER ER EES MER sees duce SER RIS sees lene EARN 11 InstallatiOra Ase vesacves sas
26. Thermo Wellwash Thermo Scientific Wellwash 4 Mk 2 Rev 2 4 Cat no 1507340 Thermo Wellwash Thermo Scientific Wellwash 4 Mk 2 Cat no 5160772 115 V 5160770 230 V or 5160771 100 V User Manual Rev 2 4 November 2008 Cat no 1507340 Copyright Copyright 2008 Thermo Fisher Scientific All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks 9 and Wellwash is a registered trademark of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions or for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Contact information Thermo Fisher Scientific Oy P O Box 100 Fl 01621 Vantaa Finland Tel 358 9 329 100 Fax 358
27. Wash reagent E SE l A E E 37 abi rr 41 49 unequal dispensing or aspirating syrror irs rres errar ay arrana eee eene nnne nhe hne hne ehh rne 49 Washer UME onedeveunieieetens aes EE E EEE da aal ous ieee ake bana NENE 10 Waste bottle vo Rua A ERR CRE TER REINES 10 37 overfilling precautior s is ox RE RR A AA ae a ated 19 Pp m 13 15 54 Workirig area 3 oie eee E rpI le apr epe Par i e NAE AENA PEPPSI pego ede pe EIT o Dpto 15 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 62 Chapter 13 Glossary and Abbreviations 13 Glossary and Abbreviations EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions EN European Norm EU European Union IEC International Electrotechnical Commission Microplate A rigid or framed polystyrene plate with microwells in different well formats e g 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Priming Completely filling the pump tubing and syringe with bubble free fluid to allow sustained reproducible pumping action The air in an unprimed line acts as a spring adversely affecting accuracy and precision Reagent A substance or solution used to produce a characteristic chemical reaction in a sample that allows an anal
28. ate washer comprising pump and washer units The instrument is supplied with both 8 and 12 way wash heads four program cards and an accessory pack Your local Thermo Fisher Scientific representative can arrange instrument training at the commissioning of the instrument for extra charge if required 3 1 Intended use The Wellwash 4 Mk 2 is a microplate washer for 96 well plates It can be used in research or routine test laboratories by trained personnel to wash Microtiter microplates and strips in 96 well plate format that meet the SBS standards For verification of the entire system it is recommended that Good Laboratory Practices GLP be followed to guarantee reliable analyses Use for self testing is excluded If the assay performance is essential for the analysis the test result has to be ensured with internal quality controls or with an alternative test 3 2 Principle of operation The instrument provides the following wash functions initiated through the keypad and control switches or a preprogrammed wash card 1 Liquid dispensing into wells microplate or microstrip 2 Liquid aspiration from wells microplate or microstrip With their unique and proven design the coaxial wash heads of the Wellwash 4 Mk 2 ensure excellent washing efficiency and low residual volumes of less than 5 ul For clean delivery of washing buffer the inner dispensing tubes are made of stainless steel A continuous vacuum in the outer tube prevents c
29. ation ID e RESET push button 12 e When pressed the RESET button will reset the washer to its pre programmed state An audible tone will sound when pressed to confirm operation Grant un POWER indicator RESET This green LED is located at the bottom left of the keypad and when illuminated indicates that the POWER switch is ON VAC FAIL indicator o 0 POWER VAC FAIL A yellow LED which when flashing indicates that the waste bottle is full or that there is a vacuum failure Fig 4 2 Keypad Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 12 Chapter 5 Installation 5 Installation 5 1 Installation check list The section on installation will contain an outline of the points mentioned in the check list below Table 5 1 Installation check list Tick Item Unpack the Wellwash 4 Mk 2 instrument carefully Refer to 5 2 1 Keep the original packaging and packing material for future transportation Check the delivery for completeness Refer to 5 2 2 Check for damage during transport Refer to 5 2 3 Place the instrument on a normal laboratory bench taking into account both the environmental and technical prerequisites Refer to 5 2 4 and 5 2 6 Leave sufficient clearance on both sides and at the rear of the unit Install the instrument Remove the transit screws Refer to 5 3 step 1 Check the voltage and frequency of the electrical supply Refer to 5 3 step 2 Remove the pinch valve
30. been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 3 Decontamination procedure Caution DO NOT use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 54 Chapter 9 Technical Specifications 9 Technical Specifications 9 1 General specifications General specifications Overall dimensions Washer unit Pump unit 30 cm x 18 cm x 16 cm 11 8 x 7 1 x 6 3 41 cm x 22 cm x 12 cm 16 1 x 8 7 x 4 7 Weight Total 10 kg 22 lbs Washer unit 4 5 kg 10 lbs Pump unit 5 5 kg 12 lbs Mains power supply 220 240 Vac 50 Hz 110 120 Vac 60 Hz 100 Vac 50 60 Hz Power consumption 300 VA Heat dissipation 1024 BTU Operating cycle Continuous Fuse requirements Washer unit Pump unit 220 240 Vac 50 Hz 2 x 0 5 A 250 V UL 198G Time Delay 2 x 1 0 A 250 V UL 198G Time Delay 100 110 120 Vac 2 x 0 5 A 250 V UL 198G Time Delay 2 x 2 0 A 250 V UL 198G Time Delay Operating conditions Temperature Relative humidity Indoor use Operating range 5 C to 40 C 15 C to 25 C 10 C to 70 C packed in transport packaging Full specification range Storage temperature range Transport temperature range 40 C to 70 C packed in transport packaging
31. cement 8 3 Limitations warnings and cautions This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety 8 3 1 Liquid reservoir IMPORTANT Due to the nature of the materials used for the manufacture of our reservoir bottles our suppliers have recommended that they are replaced after two years of use This replacement policy must be more frequent if the bottles are stored or used in direct sunlight for long periods of time Thermo Fisher Scientific Oy is aware of a potential leak in the pressure bottles when they have been subjected to long periods of UV radiation Although this occurrence is very rare our customers are urged to follow this piece of advice Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 Cat no WW934 1 See Chapter 10 Ordering Information 8 3 2 Electrical A Ensure that the mains supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The mains plug should only be inserted into a socket outlet provided with a protective ground contact The protective action must not be negated by use of an extension cable without a protective ground wire Warning DO NOT replace fuses without first disconnecting the mains supply cable Only us
32. covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 3 Warning If for any reason you need to move the instrument release first the pressure from the tubing by slightly opening the bottle cap Let the hissing sound end and then detach the yellow and blue coded tubes from the instrument 7 1 4 Immediate A 7 1 5 Tubing A Although the Wellwash 4 Mk 2 is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled aqua Spillages of liquid on the carrier slideway should be wiped off promptly as this may cause the plate carrier to drag during operation Caution DO NOT use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish Inspect the condition of the tubing regularly When you notice that a tube is becoming harder and it is no longer clear it should be replaced Only use tubing supplied by Thermo Fisher Scientific Oy The instrument is supplied with spare tubes Fig 5 2 See Chapter 10 Ordering Information Warning Never detach a tube without first releasing the pressure by slightly opening the bottle cap Let the hissing sound e
33. e The wash heads are packed separately Install them before use 5 Install first the four 4 small O rings to the wash head holders two 2 on both sides Fig 5 6 Fig 5 6 Installing the O rings to the wash head holders Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 18 Chapter 5 Installation 6 Slide the wash head between the holders Fig 5 7 Note The wash heads will only fit into the end slots when the raised flange on the wash head is facing the front Fig 5 7 Sliding the wash head between the holders 5 3 1 How to install the pump unit and washer unit connections Fig 5 8 Pump unit with tubes installed 1 The wash bottle with the black connections is located on the right hand side of the pump unit and the waste bottle to the left All the tubing and connections are color coded to ensure correct connection Table 5 2 Table 5 2 Silicon tubing color codes Pressure feed from the pump to the wash bottle Black Pressuring wash reagent from the wash bottle to the washer Yellow unit Red Vacuum feed from the pump to the waste bottle Blue Waste feed from the washer unit to the waste bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 19 A Chapter 5 Installation 2 Connect the pressure feed from the pump on the right to the wash bottle using the black coded tube 3 Connect the vacuum feed tube red from the pump to the waste bottle
34. e program card into the slot Position the plate carrier for priming Turn the instrument ON Place the microplate onto the plate carrier Remove the microplate from the plate carrier Turn the instrument OFF Thermo Scientific Wellwash 4 Mk 2 User Manual Appendix A Wellwash 4 Mk 2 Quick Reference Guide Ensure that the instrument is switched OFF Open the wash bottle cap Fig 6 1 very carefully to release any residual pressure Let the hissing sound end prior to opening the cap all the way Fill the wash bottle with appropriate washing reagent and tighten the bottle cap firmly Fig 5 12 Select either one of the preprogrammed wash cards or the programmable switch card Section 6 5 Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction of the program may occur Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 The POWER indicator on the keypad Fig 4 2 illuminates and a countdown sequence will begin on the row indicator LEDs The PRIME indicator flashes until you prime the system The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within fifteen seconds of priming then the pump will automatically switch OFF The instrument will then enter the idle
35. e fuses specified by Thermo Fisher Scientific Oy Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 52 Chapter 8 Troubleshooting Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders are prohibited covers or the removal of components is likely to expose live parts Before the instrument is opened for any adjustment replacement maintenance or repair disconnect it from all voltage sources by disconnecting the mains supply cable Warning When the instrument is connected to the mains supply the opening of Any adjustment maintenance or repair of the opened instrument under voltage should be avoided if possible but if unavoidable should be carried out only by a skilled technician aware of the hazard The same precautions applicable when using any electrical equipment should naturally be observed with this instrument DO NOT touch switches or electrical outlets with wet hands Switch the instrument OFF before disconnecting it from the mains supply 8 3 3 Mechanical and environmental Mechanical The wash head is a free moving mechanical device controlled by the instrument and not directly by the operator It is designed for hands off operation and should be used as such Never reach into the workspace while the instrument is performing a wash cycle If it is necessary to stop the op
36. eagent The wash head is factory set at a nominal height to suit the most commonly used microplates Should adjustment be required follow these instructions 1 Make sure the wash bottle is empty to facilitate the adjustment Start a simple wash routine and wait until the wash arm assembly starts to slide backwards Turn OFF the instrument from the power switch at the back of the pump unit The wash head is now in the raised position A stainless steel head lift rod is located on the left hand side of the washer unit immediately above the keypad There is a domed nut at the top of the rod with a locknut underneath Perform the adjustment using the two spanners 5 5 6 0 supplied with the instrument Fig 5 14 Loosening the locknut with spanners 4 Loosen the locknut Fig 5 14 by keeping the 6 0 spanner stationary while turning the 5 5 spanner clockwise Adjust the height by rotating the domed nut clockwise to lower the wash head and anticlockwise to raise the wash head Set the tips of the wash head to approx 1 5 mm to 2 0 mm above the height of a microplate on the plate carrier when the wash head is in the raised position Lock the domed nut into position with the locknut When locking the domed nut into position keep it still with the spanner 6 0 and only rotate the locknut anticlockwise using the spanner 5 5 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 23 Chapter 5 Installation 5
37. eck the tubing for kinks Check that the pump unit is switched ON Check that the pump unit is correctly connected to the washer unit Check the pump unit fuse and replace it if required 3 Carrier does not move when START is pressed audible warning sounds 3 times Program card not inserted No row is selected Insert the program card 6 5 Select the row number of the last row to be washed 4 Nodelivery of wash reagent into wells Silicon tube in pinch valve constantly pinched Air leaks at the wash bottle cap Tubing leaks Push the pinch valve and pull the tubing through the valve and reform 7 1 5 Tighten cap replace gasket if necessary Check the tubing and tube connectors 5 Too small dispensing of wash reagent into wells Constricted or clogged tubing Check the tubing and tube connectors for kinks and obstacles 6 Unequal dispensing or aspirating of wash reagent Microplate incorrectly positioned on the plate carrier Strips incorrectly installed in the holder One or more dispense tips clogged Check and adjust the position of the plate carrier Check that the strips are correct and adjust as necessary De clog the washer tip s 7 1 9 7 Overfilling of wells during washing Wash head height requires adjustment Air leaks at the waste bottle cap By using the spanners supplied adjust the wash head height by loosening the locknut
38. ectives the recyclers in your country and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS Regarding the original packaging and packing materials use the recycling operators known to you For further information contact your local Thermo Fisher Scientific representative Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 48 Chapter 8 Troubleshooting 8 Troubleshooting Warning DO NOT use the instrument if it appears that it does not function properly 8 1 Troubleshooting guide The problems covered below in Table 8 1 are considered as faults that require repair or corrective repair If the installation procedure is carefully followed no faults should arise However if problems occur or reoccur contact authorized technical service immediately Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 49 Table 8 1 Troubleshooting list Chapter 8 Troubleshooting Error Cause Action 1 No POWER ON indicator Mains lead not connected Connect the lead to an appropriate supply Fuse blown Replace the fuse The pump unit POWER Switch the POWER switch switch is OFF ON 2 VAC FAIL illuminated Wash bottle is full Empty the waste bottle Air leaks Check the bottle caps for Constricted tubing Pump failure leaks and tighten as required Ch
39. eed Ae Liked ee EP dee A deeper bet Pee ete petere Pelo eo eas 42 Trarnsport datmiage 1 cierre veto ee rre ERE A A FIRME ais END 14 Transport LOCKE WS tesoros os cs as Ras cias dado set 10 13 16 transport package wicks can nor oe iii 12 14 Item 48 Eres iie ieeshPaiicbe e 49 Tubes COLON CIES aa testet Tei i Ae Id eia Rig d Ies au Ee UT 18 UNPINGHING oat nO ES Ud UEM UdeMM reme 41 TWO Way Witches sti VIUERE UNES RI ERR ROS UIS VEI Tee oes Wate VRIN TURPE DURS UR TNR 31 U Unpackiha LCLLP m 12 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 12 Index Using after prolonged shutdown ccccc ccc ece nce ne eee e neon enone eee a i 34 V Vacuum fail iNdiCatOr ii ASEE RERE ESKE RE R ENAERE REEK N RER EAEE RETS 11 Vacuum Fail indicators ico a re ran das oeses cs seeded REA VA ERRASSE RAS EYRR RASSE RR ERO nes 27 28 49 Voltage 2 A EPA Site E cass HER ESPRARANRESR RAN ERR REN ESPACES daha idvicte SEPA HEN ERR ERR T 15 Mn PET 33 WwW TESTER EEEE EERS EE 30 Lll nace Er E E ii RES 12 16 57 Wash armcassembly oe in nu i s EEE E EEEE GEES AS AA AR 10 Wash bottle vicio ni 10 iristallatiori zs as E EE ea eee es 21 Wash head A deus E D E RU 22 holder sass DP c EDS 17 installation o ose dere tene tov e dees sine be deed yv eva A vedete dre bo Ce doudou dee des 18 23 A UL 42 Wash mode tit p ETE 31
40. elega die de 44 Disposal of materials errore ter epe ER ER ORT epe ERR E e irr epe ERE Red 46 Decontamination procedure ceeeeeeeeeeeeee eese hene eene het shes enn 46 How to decontaminate the instrument o ooocococcconcconnconanonanonanonanonaronanons 46 How to pack for Service ci 47 Mi ce ML mm 48 Disposal of the instrument assessuri EEEE EERE ehe ehe 48 itro bleshooting iii ide 49 Troubleshooting guide villana 49 Service request protocol oooocooococcconnconcconnconoconoronoronnrono raro ern eher enne 52 Limitations warnings ANd cautions oooocoocconcconcconnconnconnconnconnconnconnconnconnoos 52 Liquid reservoir IMPORTANT cesceececceccescesceeceee he hehe hehehe nnne 52 Electrical 42 5 ER NER IER REESE EC 52 Mechanical and environmental cecessseeeseeeseeeee e eee ehe eene er en 53 Defects and abnormal stresses ceseseseseeeseeeseeeee e eene ehe hene 54 Before operation rero eoa eode pe ER cios 54 lici 54 OPEN d E ra XR IROEI FREE ERE RU I EO E nut edede ras PR DET ERI vase 54 Cleanliness er coeeo cir er poor ote e oo ve ov poo we poorer diae 54 Technical Specifications sensis nseries egere ces uos ace asssacasecn acu Gon acude 55 General Specifications cc sic ri or n a oi ERAS ER oA aids die dale NES ERR CER ga ai 55 Safety specifications TTE 56 In conformity with the requirementS oooococccoccconcnonaconanonanonaronaronanonanonanons 56 Orderi
41. er and disconnect the mains supply cable 2 Slide the washer and pump units open and disconnect the pressure and vacuum tubing from the washer unit by slightly opening the wash bottle cap Let the hissing sound end prior to Warning Never disconnect the pressure tubing without first releasing the pressure disconnecting the tubing 3 Locate the fuse holders at the rear of the washer and pump units 4 Onthe washer unit use a screwdriver to remove the fuse holder cap Turn the screwdriver counterclockwise about 45 to release it and then pull it out Fig 7 8 PRESSURE VACUUM Fig 7 8 Opening the fuse holder of the washer unit 5 Remove the faulty fuse and discard it PRESSURE VACUUM Fig 7 9 Washer unit fuse and fuse holder Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 44 Chapter 7 Maintenance 6 Ensure that the replacement has the same rating as appears on the rating label on the rear panel of the washer unit Only use fuses specified by Thermo Fisher Scientific Oy 7 Insert the new fuse to the fuse holder and push the holder into its place Turn the holder clockwise about 45 with the screwdriver 8 Onthe pump unit use the screwdriver to gently pry the fuse folder open THIS Equip BEC MENT ae CTED TO A PROTECT TH MAINS supp v VE VACUUM E ADJUST Fig 7 10 Opening the fuse holder of the pump unit 9 There are two fuses inside the holder The out
42. er one is a spare fuse and the inner one is the one in use e BAT TAY Mu P3 TO A VYxXOTY CTI a Wass SUPPLY F COVVIE F1 O KHNA AUST Fig 7 11 Pump unit fuse holder opened POWER 10 Remove the inner fuse and discard it 11 Ensure that the replacement fuse have the same rating as appears on the rating label on the rear panel of the pump unit 12 Insert the new fuse the spare fuse to the inner slot of the fuse holder and push the holder into its place 13 Reconnect the tubing and the washer and pump units Reconnect the instrument to the mains supply If the fuses blow again contact authorized service See Section 8 2 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 45 Chapter 7 Maintenance Note Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders are prohibited 7 2 Disposal of materials AN When disposing of used material follow the Good Laboratory Practices Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used microplates microstrips disposable gloves syringes disposable tips etc as biohazardous waste 7 3 Decontamination procedure gt gt D Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instruc
43. eration of the equipment press the RESET button on the keypad Never open the wash bottle detach any of the tubing or press the black top of the pinch valve without first releasing the pressure by slightly opening the bottle cap Let the hissing sound end prior to proceeding Environmental Infectious clinical samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to wash the reaction wells without getting into direct contact with these fluids However wells that have been in contact with potentially hazardous fluids must be handled before and after the wash process and this should be done with utmost care Hand and eye protections should always be worn as well as corrosive resistant laboratory coats A If you are using a washing reagent that is either volatile or easily splashing or gt D when you are washing microplates with samples of human or animal origin keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood When spills get into contact with the carrier slideway it should be decontaminated with a hard surface disinfectant see Section 7 3 Observe Good Laboratory Practices GLP when handling potentially hazardous samples Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 53 Chapter 8 Troubleshooting 8 4 Defects and abnormal stresses Whenever it i
44. es 7 4 How to pack for service When you ship the instrument for service remember to Inform about the use of hazardous materials Decontaminate the instrument beforehand Screw the transit screws into the bottom of the instrument to the correct shipment location Pack the instrument according to the enclosed packing instructions Section 7 4 1 Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges Enclose a dated and signed Certificate of Decontamination Appendix B both inside and attached to the outside of the package in which you return your instrument or other items Enclose the return authorization number RGA given by your Thermo Fisher Scientific representative Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department See Section 9 1 for details on storage and transportation temperatures See also Section 8 2 Service request protocol Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 47 7 4 1 Packing Chapter 7 Maintenance The Wellwash 4 Mk 2 comprises two units that are packed in a single box For packing the washer and pump units follow the procedure below 1 Place the box on the floor and open the top Put the bottom part of the package shock absorber at the bottom of the box 2 Wrap the
45. g the instrument from the mains 3 Perform appropriate corrective actions However do not touch the interior of the instrument 4 Contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 35 Chapter 7 Maintenance 7 1 Regular and preventive maintenance 7 1 1 Service contracts It is strongly recommended that this instrument be regularly maintained and serviced every twelve 12 months on a contract basis by trained service engineers of the manufacturer This will ensure that the product is properly maintained and that it gives trouble free service For more details contact Thermo Fisher Scientific s service department 7 1 2 Maintenance check list The check list below contains a recommended maintenance schedule Table 7 1 Maintenance check list Item As required Daily Weekly Monthly Yearly Wipe the case of the instrument Cover the instrument with the dust cover after use Clean the instrument and the keyboard with a cloth dampened with mild detergent followed by deionized distilled aqua See Sections 7 1 3 and 7 1 4 Check the condition of the tubing and the wash and waste bottles See Sections 7 1 5 and 7 1 6 Check the pinch valve See Section 7 1 8 P Unclog the wash head tips See Section 7 1 9 E Change the fuses when blown See Section 7 1 10 Decontaminate
46. he enhn 32 Fig 7 1 Liquid level sensor and liquid trap bottle cover eese 38 Fig 7 2 Opening the liquid trap bottle cover sese nne 39 Fig 7 3 Pulling the thick tube out of the liquid trap bottle suesss 40 Fig 7 4 Pulling the thin tube out of the liquid trap bottle eseess 40 Fig 7 5 Opening the liquid trap DOttle cece cece cece cence cence eeneeeeeeeeeeneeeeees 41 Fig 7 6 Unpinching the tube c cece cece cence cee ee cee ee eene enhn ehh enhn 42 Fig 7 7 De clogging the wash head ccsssssssseeeeeee eee ehe eene enhn 43 Fig 7 8 Opening the fuse holder of the washer unit eee 44 Fig 7 9 Washer unit fuse and fuse holder eee en 44 Fig 7 10 Opening the fuse holder of the pump unit esee 45 Fig 7 11 Pump unit fuse holder opened ccce eene nene 45 List of Tables Table 5 1 Installation check list cies cies e Leer tet orna eer ere se eres EE veces 13 Table 5 2 Silicon tubing color codes cece cece cence ene ee eee cena ee eene ehh enhn 19 Table 6 1 Operational checklist cesseessesssesseeeseeeee eee ee eene mene 26 Table 6 2 Number of washes after a soak period eee 33 Table 6 3 Aspiration after the final wash ccsssesssseeeeeeeee eene enhn 33 Table 6 4 Soak period in MINUtES cece cece
47. he instrument should be located and operated The Wellwash 4 Mk 2 does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Warning DO NOT operate the instrument in an environment where potentially damaging liquids or gases are present 5 2 5 Things to avoid DO NOT smoke eat or drink while using the Wellwash 4 Mk 2 Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Be sure the working area is well ventilated Never spill fluids in or on the equipment Only use the instrument for its intended purpose Refer to Section 3 1 5 2 6 Technical prerequisites The washer and pump units require no special site preparations A normal laboratory bench close to a mains supply socket is quite suitable The net weight of the instrument is 10 kg 22 lbs Check that the voltage label on the rear panel of the pump unit conforms to the supply voltage used in the laboratory The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz see Section 5 3 step 2 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 16 Chapter 5 Installation 5 3 Installation procedure Any suitably skilled person using standard tools may carry out the following procedure 1 Rest the pump uni
48. holder Refer to 5 3 step 4 Install the wash head Refer to 5 3 steps 5 and 6 Connect the tubing and the liquid level sensor cord to the pump unit Refer to 5 3 1 steps 1 to 5 Connect the washer unit to the pump unit Refer to 5 3 1 steps 6 and 7 Fill the wash bottle and tighten both bottle caps Refer to 5 3 1 steps 8 and 9 Slide the plate carrier to its place Refer to 5 3 1 step 10 Connect the mains supply cable cord to the mains input socket Refer to 5 3 2 Carry out the operational check Refer to 5 4 OUOOOKOOOKOOO O OD O 5 2 What to do upon delivery 5 2 1 How to unpack A Move the unpacked instrument to its site of operation Unpack the Wellwash 4 Mk 2 instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package e the Warranty Certificate card e the packing instructions packing list e the Transportation discrepancy report e the User Manual Caution DO NOT touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty Fig 4 1 shows the pump and washer units in their entirety Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 13 Chapter 5 Installation Lift out the washer and pump units removing the sha
49. ings used in the documentation Note Marks a tip important information that is useful in the optimum 1 operation of the system or an item of interest Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 2 About the User Manual 2 About the User Manual This User Manual has been written for the actual user e g laboratory technician and provides information on the Thermo Scientific Wellwash 4 Mk 2 microplate strip washer including installation and operating instructions Read the manual in its entirety prior to operating the instrument This User Manual has been designed to give you the information you need to e Review safety precautions e Install the Wellwash 4 Mk 2 e Carry out washing procedures e Perform basic maintenance procedures e Troubleshoot the instrument performance e Maintain the instrument This User Manual also describes features and specifications of the Wellwash 4 Mk 2 hardware and on board software Chapter 6 explains the operating procedures The user should be familiar with the contents of Chapter 7 on maintenance procedures that may be performed by the user For warranty and ordering information refer to Chapters 10 Ordering Information and 11 Warranty Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 3 Introduction to the Wellwash 4 Mk 2 3 Introduction to the Wellwash 4 Mk 2 The Wellwash 4 Mk 2 is a semiautomatic micropl
50. licei 67 NOteS M 68 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Table of Contents Table of Contents 1 2 3 3 1 3 2 3 3 4 4 1 5 5 1 5 2 5 2 1 5 2 2 5 2 3 5 2 4 5 2 5 5 2 6 5 3 5 3 1 5 3 2 5 3 3 5 3 4 5 4 6 1 6 2 6 3 6 4 6 5 6 5 1 6 5 2 6 6 6 7 6 7 1 6 7 2 6 8 7 1 7 1 1 7 1 2 7 1 3 7 1 4 7 1 5 7 1 6 7 1 7 7 1 8 Safety Symbols and Markings sccscccscccccccccccscccsccesccesccesccesscesseesseessens 7 About the User Manual ceeeeeseeeeseeeeeeee eene hehehe hens enses sess sess senos 8 Introduction to the Wellwash 4 MK 2 cccscssceccsscccccsccscescescescescessessesseses 9 Intended se isse veya E esa er RR S ER RES ve vacate M ER E 9 Principle of operation 2 eee tero e oe roe ore tede toe tree ases ty sedeaer ses roce tese nistx v aee bees 9 Advantages of using Wellwash 4 Mk 2 csssssesseeeseeee eene ene ehe ehe ete eren 9 Functional Description 22 012 02320 9c P eec uee inire o Morea rep enn reu roug RR ea eG 11 Gontrols and indicators eor one sees px Er ERE NES yy ER EET se dace dey gene NES a EROR ES eee 12 nelli Ec M 13 Inst llation checklist uox cepere donada epu up r E eg 13 What to do upon delivery cesssesseeeseeeeeeeee e eee enhn he ehe te ehe nene 13 HOW tO unpack 2 oen RR et RE IER A ER rU TERR 1
51. lling selected rows in the sequence determined by the program card fitted On completion of the protocol the plate carrier will come to rest with the wash head lowered into the priming reservoir Rev 2 4 Cat no 1507340 27 Caution gt gt Note ap Note Caution Caution Caution Note Caution Dalia Warning Warning Warning Warning Chapter 6 Operation If the instrument is used in a manner not specified by the manufacturer the protection provided by the instrument may be impaired See Section 9 1 General specifications Handle the microplate with care to avoid any contamination of the instrument and the subsequent specimens The contamination of the specimen will lead to incorrect results If the contents of the microplate does spill however follow the decontamination procedure presented in Section 7 3 Also follow the system supplier s instructions in regard to the reagent kit and specimen handling in the event of contamination Never open the wash bottle detach any of the tubing or press the black top of the pinch valve without first releasing the pressure by slightly opening the bottle cap Let the hissing sound end prior to opening the cap all the way If you are using a washing reagent that is either volatile or splashes easily keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood When washing
52. nd prior to detaching any of the tubes 7 1 6 Reagent waste bottles Due to the nature of the materials used for the manufacture of our reservoir bottles our suppliers have recommended that they be replaced after two years of use This replacement policy must be more frequent if the bottles are stored or used in direct sunlight for long periods of time Thermo Fisher Scientific Oy is aware of a potential risk of leakage in the pressure bottles when they have been subjected to long periods of UV radiation Although Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 37 Chapter 7 Maintenance this occurrence is very rare our customers are urged to follow this piece of advice Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 Cat no WW934 1 Spare O rings for the bottle plugs are included in the spare parts bag delivered with the instrument see Fig 5 1 item 6 See Chapter 10 Ordering Information Ensure that the waste bottle is emptied regularly and at least every time the wash bottle is filled Do not let the liquid level exceed the level mark on the bottle The waste bottle is fitted with a liquid level sensor The sensor prevents the use of the instrument after the waste bottle is filled to the mark 7 1 7 Liquid trap bottle The liquid trap bottle prevents liquid from entering the pump and it should be kept dry at all times Liquid should enter the liquid trap bottle o
53. ng 8 2 Service request protocol If the Wellwash 4 Mk 2 requires service contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department DO NOT under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your Thermo Fisher Scientific representative or distributor will take care of sending a complaint form Complaint order to Thermo Fisher Scientific s service department The Complaint order contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill in and forward the Complaint order to Thermo Fisher Scientific s service department Check Section 7 4 How to pack for service You will find instructions on how to proceed before shipping the instrument for service Check that any necessary decontamination procedure has been carried out before packing See Section 7 3 Decontamination procedure Ensure that the Certificate of Decontamination see Appendix B as well as the return authorization number RGA are sent with the instrument Thermo Fisher Scientific s service department will keep you up to date with the progress of service and provide you with any further details you might need e g on maintenance serviceability troubleshooting and repla
54. ng Informati n initial 57 list of ACCESSOMES wees ecules cece eee cscs casi ba SACRAS ERCERNESPRNRE ES ERCEENESSE REUS ANTR 57 List of recommended spare parts ccecceecceeccenccenceenseenseeneeenseenseenseeneees 57 Warranty Certificate isis 58 Warranty And det 58 Indexes nm 59 Glossary and Abbreviations scccscccccscccccccccecccccesccccesccceesssceescseesssses 63 Keywords for web pages ccccecccccencencencees ene hehehe teeth theses esset se satu 63 Literature ie a RE OX RE ERROR AAA ER AAA PATERE 63 Wellwash 4 Mk 2 Quick Reference Guide eeeeeeeeee eee eee eene nennen 64 Certificate of Decontamination ccsceccsccsccsccccccccesccsccscescescescessessesees 65 Wellwash 4 Mk 2 Feedback FOrim csscssesccsccsccsccsccsccsccesccscescescescesseseess 66 Addresses m cT 67 NOteS 0 5iriooo sete ese ora OP ESTA pe eer eoe SERE ee Uer Pesos ors sce Ue once EURO Diis 68 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 List of Figures and List of Tables List of Figures Fig 4 1 Wellwash 4 Mk 2 microplate washer ccsssseseseeeee eene eene 11 Fig 4 2 Keypad osse ee resur te tede errasse quee eee qec see ge ve outer ro ve ao eR sin 12 Fig 5 1 Accessories included with the instrument ccce 14 Fig 5 2 Spare tubing vacates eee I savas as TRI RR ERE Ere Rer R t Ri RE 15 Fig 5 3 Wash arm assembly tubing ccssssseeeseeeeee
55. ng sound end prior to pushing the valve top NA weuwasn SP Fig 7 6 Unpinching the tube If the washer is run now the liquid will flow normally It may be necessary to replace the tube after extended periods of use due to fatigue Use the spare tubes supplied with the instrument Fig 5 2 item 3 1 Switch OFF the instrument and make sure there is no pressure in the wash bottle by opening the wash bottle cap slightly 2 Press the black top of the valve to release the tube and slide the tube gently out of the pinch valve through the slit on the side 3 Disconnect the tube from both ends and replace with a new one 4 Press the black top of the valve and slide the new tube back into the valve through the slit Make sure the tube is properly in place through the valve and is not pinched especially on the rear side of the valve 7 1 9 Uncloggins tips The dispense aspirate tips of the wash head may become clogged with salt deposits To clear them switch OFF the washer lift up the wash arm assembly Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 42 Chapter 7 Maintenance and unclog the tips using the small de clogging tool provided see Fig 7 7 Push the de clogging tool into the thin metal tube Fig 7 7 De clogging the wash head Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 43 Chapter 7 Maintenance 7 1 10 Fuse replacement 1 Switch OFF the wash
56. ng the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals Please include decontaminating solution used Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 65 Appendix C Wellwash 4 Mk 2 Feedback Form Please send to Thermo Fisher Scientific Oy Fax 358 9 32910415 Appendix C Wellwash 4 Mk 2 Feedback Form Cat no Serial no PURCHASED BY PURCHASED FROM Company Organization Distributor Department Address Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr Ol Mrs L1 Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions O O O L1 Instrument User manual O m m m Operational reliability O O O L1 Design O L1 L1 O Ease of use O O O O Operational costs O O O O Customer support O O O O
57. nly if the liquid level sensor is not properly connected Fig 7 1 item 1 and the waste bottle overflows If that happens follow immediately the steps below 1 Switch OFF the instrument and make sure there is no pressure in the wash bottle by opening the wash bottle cap slightly 2 Open the black screw on the cap of the liquid trap bottle cover Fig 7 1 item 2 1 Liquid level sensor 2 Liquid trap bottle cover screw Fig 7 1 Liquid level sensor and liquid trap bottle cover Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 38 Chapter 7 Maintenance 3 Release the cover by slightly lifting the tube and slide the cover then upwards along the tube Fig 7 2 Fig 7 2 Opening the liquid trap bottle cover Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 39 Chapter 7 Maintenance 4 Pull the thick vacuum tube with the 90 angle out of the liquid trap bottle Fig 7 3 Pulling the thick tube out of the liquid trap bottle 5 Pull the thin tube out of the liquid trap bottle a A A Y 7 PF Fig 7 4 Pulling the thin tube out of the liquid trap bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 40 Chapter 7 Maintenance 6 Let the red coded tube remain connected to the bottle cap and take the bottle out unscrew the liquid trap bottle cap and empty the bottle Fig 7 5 Opening the liquid trap bottle 7 Clo
58. ntially dangerous plates Use proper protective clothing Use disposable gloves Ensure that the working area is well ventilated Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 28 gt b gt D Chapter 6 Operation Caution Never spill fluids in or on the equipment Prevent any liquid from entering the instrument Caution Immediately wipe away spilled liquids from outer surfaces to prevent damage and wipe over with a mild detergent Caution Keep the instrument free of dust and other foreign matter Clean the plate carrier and the plate carrier slideway and keep them dry to prevent jamming 6 3 Priming procedure Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 Press the PRIME button The wash head will lift and the pinch valve will open for one second allowing wash fluid to flow into the priming reservoir The wash head will then lower itself to empty the priming reservoir The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within ten seconds of priming the pump will automatically switch OFF The instrument will then enter the idle state waiting for instructions from the keypad and program card If the pump fails to start or if the bottle caps are loose an audible warning will be heard and the VAC FAIL indicator will illuminate This condition will clear once the pump has started If
59. of the program may occur If a run is started without a program card or any row selection the instrument will signal an error by sounding the audible warning device 3 times If a run is started with the rows selected but with no program card inserted the instrument aspirates the rows selected and returns to its idle state with the wash head lowered into the priming reservoir If a run is started without any rows selected but with a program card fitted the instrument will aspirate all the rows in accordance with the card configuration i e 8 way or 12 way wash head The preprogrammed wash cards will dispense and aspirate the plate row by row for the number of times indicated on the card There are four cards which can be inserted either side up to perform the wash routine indicated on the side facing up en a Fig 6 2 Thermo WELLWSH4MK2 ELECTRON CORPORATION THIS WAY UP WW006 13C1 Preprogrammed card three washes with an 8 way wash head Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 31 Thermo Scientific Wellwash 4 Mk 2 User Manual Chapter 6 Operation Contained on the programmable switch card are four 4 rotary type and nine 9 two way type switches each marked with their function The setting of these switches controls the function of the washer enabling a wide variety of protocols to be performed WW 006 10C1 ELECTRON CORPORATION 4 SOAK
60. ottle cap firmly see Fig 5 12 Select either one of the preprogrammed wash cards or the programmable switch card see Section 6 5 Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction of the program may occur Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 The POWER indicator on the keypad Fig 4 2 illuminates and a countdown sequence will begin on the row indicator LEDs The PRIME indicator flashes until you prime the system Section 6 3 The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within fifteen seconds of priming then the pump will automatically switch OFF The instrument will then enter the idle state waiting for instructions from the keypad and program card Check that the correct wash head 8 way or 12 way is fitted Check that the plate is placed properly onto the plate carrier Fig 5 16 Select the row number of the last row to be washed If no row is selected the whole plate will be washed The instrument performs the wash sequence After pressing the START button there may be a delay of up to 15 seconds before the run begins to allow the reagent and waste bottles to reach the working pressure The plate carrier will advance into the washer aspirating and fi
61. own procedure 2 55 deor roter ato te unen hern ehe rera aaa eene Ee a e 35 Daily shutdown PrOCEdUre ecce ccc eec cence ene eene eens eene enhn eese ens 35 Long period SHULCOWN ccccccecceccceeccenceeeceenseeeseeeseeeseeeseeeseeeseeeseeeseees 35 EMEFgencySItUALIONS scaosssensadenesscags ER ER EPIRI ERI ERR MI IA e ERE OR Te TA 35 Maintenance eee eee re cian 36 Regular and preventive maintenance ecsseesseeeseeeee esee ehe ehe eher 36 Service contracts sese eye CR RAS TERRE CN ERAS ADA 36 Maintenance checklist ee ror Ir YR n ER ER TR I EN REESET E Y teg 36 General m 36 nius P 37 Uc 37 Reagent waste bottles o ooooooccocccoccconnconnconcconn conc Aaa PN Nri 37 Liquid trap bottle 25 2 drei rede odia e reda e ehe ri dede de 38 Pinch VIVE cual terreno tere clonic dove Henne tole en ceo euis erede x clowe Ius ure deu dee dieu y 42 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 7 1 9 7 1 10 7 2 7 3 7 3 1 7 4 7 4 1 7 5 8 1 8 2 8 3 8 3 1 8 3 2 8 3 3 8 4 8 5 8 5 1 8 5 2 8 6 9 9 1 9 2 9 3 10 10 1 10 2 11 11 1 12 13 13 1 13 2 Appendix A Appendix B Appendix C Appendix D Table of Contents Unclogeing ti 42 Fuse replacement 5 oco ice nel prae per ta pal gage Dei pese mel ER de Dei pese d
62. ped packing pieces from around the washer and place them on a flat level surface To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight The net weight of the instrument is 10 kg 22 lbs Caution When unpacking the instrument it is recommended that two people lift the instrument together taking proper precautions to avoid injury Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Note Before use remember to remove the transit screws at the bottom of the instrument and save them in the adjoining screw holes marked with the text Transit screw storage Remove and save the pinch valve holder until needed for future transportation of the instrument 5 2 2 Checking delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy The instrument is supplied with both 8 and 12 way wash heads four program cards the user manual the mains supply cable and an accessory pack The content of the accessory pack is shown in Fig 5 1 and Fig 5 2 Store the accessories in their bags until you need them
63. r to normal use 1 Check that the wash head is correctly located by means of its guide slots in the head assembly Fig 5 7 The operational check is performed faster with the 12 way wash head 2 Add approximately 2 to 3 dl of distilled aqua to the wash bottle and make sure that both bottle caps are firmly tightened 3 Switch ON the mains power supply by depressing the POWER ON switch positioned on the rear panel of the pump unit The POWER indicator on the keypad Fig 4 2 will be illuminated and a countdown sequence will begin on the row indicators The row 12 indicator will illuminate first followed by row 11 row 10 row 9 and so on at one second intervals until the PRIME indicator is reached The pressure Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 24 Chapter 5 Installation generation takes 10 seconds The PRIME indicator continues flashing to prompt the user to prime the system 4 Select the preprogrammed wash card for 1 wash and for the 12 way wash head Plug it into the slot on the right hand side of the washer unit Fig 4 1 item 13 5 Lift the wash head and slide the plate carrier onto the stainless steel slideway so that the reservoir is under the wash head Fig 5 13 6 The instrument should now be primed Press the PRIME button to initiate the sequence The wash head will lift and the pinch valve will open for one second allowing wash fluid to flow into the priming reservoir
64. ration 6 Operation 6 1 Operational checklist Table 6 1 Operational checklist Tick Item Fill the wash bottle with appropriate washing reagent Select the program card and insert it When using the programmable switch card check the settings Switch the instrument ON Prime the instrument Place a microplate onto the plate carrier Select the row number of the last row to be washed Start the wash sequence When the wash sequence has finished remove the microplate OOOO DO CODO oO O DU Shut down the instrument The following symbols are used in the flowchart below o Press the key Perform action Indicates the actions you should take Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 26 Chapter 6 Operation 6 2 Washing a microplate Fill the wash bottle and empty the waste bottle Plug the program card into the slot Position the plate carrier for priming Turn the instrument ON Place the microplate onto the plate carrier Remove the microplate from the plate carrier Turn the instrument OFF Thermo Scientific Wellwash 4 Mk 2 User Manual Ensure that the instrument is switched OFF Open the wash bottle cap Fig 6 1 very carefully to release any residual pressure Let the hissing sound end prior to opening the cap all the way Fill the wash bottle with appropriate washing reagent and tighten the b
65. rd 5 p RR YER ERR EROR AAA AAA ROIG 10 30 PUT Te Sun eve Ale cca saciea ude PD Tm 10 installation iii A ana adas 18 Q Quick Reference Guide is isis cess cece es a ro nr a OR VOR NS NON v s ds UR Aia 63 R Reagent DOTE Ei r a A ee ta Oe ee A ea eee 10 37 Reset bUttol voe oder A c vele RE E eb Ve EE EE 11 27 28 34 Rotary switclies 5 A A s die Oe ET eRe 32 Row select buttons i eee ek epe te reae ler epe o Rue ele ere ENERE E e ia e e ege EREKE 11 Row select indicators ORO EO ER OR ERROR RERO RE ERR 11 countdown sequence A 23 26 S Mea 7 Selecting DOOM AM EA 30 NE 51 SUL ELTA PPE AEE iii de 34 DVRS RA EE TONERE EE ENS ENTRE HS TANA Ne EE ds DORAN e EEEE 34 A RR RIS NRNROR VATRVRURENPRURIR AREVRURSNNR RUD VERS VRERENIS URN VERS VRIREERSURINUS 34 Site of operatiOh ressent EARS RR ENSE e 15 SOAK PSMOGes UEM 32 SPal SiPaless cocsee ER 56 Specifications BENS Al hi does M R 54 Safeby cbse TAN De o alors Ga BO E E is Rh Oh Slat BE eh ON Sars id E 55 Start DUEEOR 20 cie e Lb ELE e e chee chee nes chee chee hee chee has hee chee hed Er V 11 SATU ste sce cicts cee cetaceans ales cite clekaioleed ciety vasa ced vinbud clea A cleus same cekuneud cleus vase cel gene dee see 26 SWitcli CaEdiius cres ee there i eu ere ee ea esee A eoe ve e eh cen eere a eere e EN SEND 31 T Tips UNGLOSGING 3232s r
66. rier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 15 Chapter 5 Installation 5 2 4 Environmental requirements When you set up your Wellwash 4 Mk 2 avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables wash bottles etc e Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambient temperature range is between 5 C 41 F and 40 C 104 F preferably between 15 C 59 F and 25 C 77 F e Make sure relative humidity is between 10 and 90 non condensing Note The use of the instrument in strong direct sunlight or next to radiators and other heat sources may markedly increase the temperature prevailing at the instrument This should be taken into account when deciding where t
67. ross contamination and ensures complete liquid removal For fast and accurate selection of routine protocols the Wellwash 4 Mk 2 comes with four standard program cards 1 to 4 washing cycles as well as a programmable card This switch card permits a quick selection of all wash parameters including soak times and final wash protocols The quick release bottle caps of the Wellwash 4 Mk 2 ensure easy buffer changes An additional rinse bottle can be connected making it easier to purge wash buffer from the wash head or change to a different wash buffer Automatic pump shutdown reduces wear and noise levels If unused for 30 seconds the wash pump of the Wellwash 4 Mk 2 automatically switches off 3 3 Advantages of using Wellwash 4 Mk 2 The Wellwash 4 Mk 2 provides several advantages relating mainly to the principle of operation in that it has e Unique coaxial wash heads for trouble free washing and low residual volumes e 8 and 12 way interchangeable wash heads e Easy to program e Excellent washing performance e Quick release wash waste bottle caps e Automatic pump shutdown Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 e Effective liquid management Thermo Scientific Wellwash 4 Mk 2 User Manual Chapter 3 Introduction to the Wellwash 4 Mk 2 Rev 2 4 Cat no 1507340 10 Chapter 4 Functional Description 4 Functional Description The Wellwash 4 Mk 2 is designed to wash wells in an 8 x 12
68. s 0 1 2 3 4 5 6 7 Plate wash mode seconds Setting 8 9 A B C D E F Strip wash mode 8 9 10 15 20 30 145 60 seconds Plate wash mode 50 60 75 85 105 120 180 240 seconds WASHES Set the number of washes to be performed before a soak period Table 6 6 Table 6 6 Number of washes before soaking Setting 0 1 2 3 4 5 Number of washes 0 1 2 3 4 5 Setting 8 9 A B C D E F Number of washes 8 9 10 11 12 13 14 15 No wash only aspirate VOLUME Set the volume dispensed during washing Table 6 7 Table 6 7 Washing volume in ul Setting 0 1 2 3 4 5 6 7 Washing volume ul 0 50 100 150 200 250 300 350 Setting 8 9 A B C D E F Washing volume ul 400 450 500 550 600 650 700 750 Note The dispense volume is less than the wash volume to avoid overfilling the wells Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 34 Chapter 6 Operation 6 6 Resetting If you press the RESET button on the keypad at any stage this will stop the immediate washing sequence and reset the system to its pre programmed state 6 7 Shutdown procedure Warning Remove any microplate still on the plate carrier Dispose of all microplates and strips as biohazardous waste The wash reagents may contain buffer salts which can crystallize when the washer is not in use This may cause
69. s likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation The protection is likely to be impaired if for example the instrument 1 Shows any visible damage 2 Fails to perform the intended functions 3 Has been subjected to prolonged storage under unfavorable conditions 4 Has been subjected to severe transport stresses 8 5 Before operation Read this manual in its entirety as it contains information necessary to ensure safe operation 8 5 1 Installation The instrument should be unpacked and installed in accordance with Chapter 5 Particular attention should be paid to the correct fitting of the tubing to obviate leaks Fig 5 11 Always ensure that the electrical supply in the laboratory conforms to that specified on the voltage label on the rear panel of the pump unit 8 5 2 In operation Ensure that the waste bottle is emptied regularly and at least every time that a wash bottle is loaded The liquid trap bottle that prevents liquid from entering the pump should be kept dry at all times 8 6 Cleanliness Clean the instrument regularly as stated below 1 It is recommended that the case of the instrument is cleaned periodically to maintain its good appearance It is particularly essential that the carrier slideway be clean and dry to prevent jamming A soft cloth dampened in warm mild detergent solution will suffice 2 If any surfaces have
70. se the bottle Make sure the end of the red coded tube is about 2 cm deeper inside the bottle than the thin tube will be Put the bottle back to its place but don t push it yet all the way down 8 Insert the thin tube back through the liquid trap bottle cap so that the mark on the tube Fig 7 4 item 1 is at the level of the cap 9 Push the bottle all the way down Push the thick vacuum tube as far as it goes Its end should now be approximately at the same level as the red coded tube s end 10 Slide the cover back down and screw the black screw back on You can pull the red coded tube slightly out to make sure the cover is tightly on Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 41 Chapter 7 Maintenance 7 1 8 Pinch valve A Occasionally the tube passing through the pinch valve becomes permanently closed i e it will not return to its normal shape when the valve opens while in use This tends to occur after extended periods of non operation Release the pressure in the tubing by opening the wash bottle cap slightly To unpinch locate the valve under the wash arm assembly Press the black top of the valve to release the tube and pull the tube gently through the pinch valve a few millimeters the valve will now pinch into a new position See Fig 7 6 Warning Never press the black top of the pinch valve without first releasing the pressure by slightly opening the wash bottle cap Let the hissi
71. state waiting for instructions from the keypad and program card Check that the correct wash head 8 way or 12 way is fitted Check that the plate is placed properly onto the plate carrier Fig 5 16 Select the row number of the last row to be washed If no row is selected the whole plate will be washed The instrument performs the wash sequence Rev 2 4 Cat no 1507340 64 Appendix B Certificate of Decontamination Appendix B Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific Oy e g for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument See Section 7 3 Decontamination procedure Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B confirm that the returned items have been decontaminated and can be handled without exposi
72. t on its side and remove the transit screws the Allen cap head type is painted red using the key supplied Fig 5 1 item 5 Relocate them in the retaining holes provided Fig 5 4 Note When relocating this instrument or returning it for repair it is essential that the transit screws are refitted Failure to do so could invalidate the warranty 2 Ensure that the pump mains switch is in the OFF position After checking that the electrical supply conforms to that specified on the voltage label on the rear panel of the unit slide the frequency selector switch in the pump model Cat no 5160771 to the 50 Hz or 60 Hz position depending on the mains supply voltage frequency Fig 5 4 Other pump models do not include a frequency selector q cH o 53 e 2 TE 60Hz e FREQUENCY SOHz 2 m SCREWS MUST BE FITTEO IN THESE TWO TRANSIT POSITIONS PRIOR TO SHIPMENT e e A 1 Location of the frequency selector switch 2 Retaining holes for the transit screws 3 Transit screws during transportation Fig 5 4 Location of the frequency selector switch and the transit screws 3 Turn the pump unit back to its normal position Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 5 Installation 4 Lift the wash arm assembly the stainless steel cover Remove the pinch valve holder Fig 5 5 before use Store the wire holder for future use Fig 5 5 Pinch valve holder Not
73. tage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 56 Chapter 10 Ordering Information 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Code Instrument 5160770 Wellwash 4 Mk 2 220 240 V AC 50 Hz 5160772 Wellwash 4 Mk 2 110 120 V AC 60 Hz 10 1 List of accessories Code Product N03283 2 wash bottle 1514313 2 waste bottle WW004 1 12 way wash head WW 004 11 8 way wash head WWO004 48 Wash height adapter 10 2 List of recommended spare parts Code Product 006 612 Spare parts bag for 220 V see Fig 5 1 006 613 Spare parts bag for 110 V 006 6133 Spare parts bag for 100 V 004 023D Spare tubes bag see Fig 5 2 1210840 Spare fuses 0 5 A 1210890 Spare fuses 1 A for 220 V 1210920 Spare fuses 2 A for 100 V and 110 V Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 Chapter 11 Warranty Cer
74. tificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business s Wellwash 4Mk2 is fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of twelve 12 months from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions Warranty is invalid if products have been misused or abused For this warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above e Refurbished products which are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connec
75. tion with the use of the product or other indirect damages Full warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer This document acts as warranty certificate 11 1 Warranty limitations Consumables are not included in the warranty Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 58 Chapter 12 Index A AGGCESSOMTOS i rere ES Rea Xd rao Ev Xara LEER TERM SEP eR UE den acs TAE dead eed Recuerda das da edo Ve S ER TR 56 icio eee 14 Meter 12 Accessory PACK GE 13 ApplicatiOM eser EUER 65 A dible warning o miis arrai rna pna xe anon ee ooo ee ext RT PUE OK RANT ERROR A SER RXRAT ARN ES S E RETE 30 B slojudi Ker io pes EUER 10 Bottles umi EEE EEE EEE EE EEE 10 user EE EAEAN RNA ONEA E NaN 37 replacement interval esie re yeu enean pee seen erano RE 36 AI A IEA AIDA AAA iii oie 11 C Certificate of DecontaminatiOn i ee eren rere n eae eee e ana a ERE sicselsie deducts FARNE EXEC E EVE Ee ao 64 CHECK A AA A pe D DERE ORE ORAE AERE PRAE DERE DEAE OR SE PRAYOREE ORAE CENUPEANOMNE DEAE CREE DRAN OM DERE CEN NUM M EENUE 12 miaintence ses ene EET 35 CUS O a EE ASEA AEE oe Wen aid oid EE NE A N EAGEN 42 oin P EET 34 Color coding of the tubes eie o a erus oe EK E Ara a n dened ees E Om 18 eopcid ect rS 51 Consumables warranty LIMITATIONS cece enr nere Ran Ea M se he KESE e hehe eher nnne 57 Contamination SPECIM
76. tions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended that the complete decontamination procedure be performed before relocating the instrument from one laboratory to another See Section 7 4 How to pack for service Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in EIA tests in a negative way resulting in bad test results Caution Do not use sodium hypochlorite as a decontaminant The wash head will be damaged by it Warning Always use disposable gloves and protective clothing and operate in a well ventilated area 7 3 1 How to decontaminate the instrument 1 Prepare the decontaminant e g 200 ml 1096 formaldehyde solution 1 3 Virkon solution or 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer
77. yte to be detected and measured RS 232C EIA approved standard used in serial data transmission covering voltage and control signals The data is transferred serially one digital bit at a time via one path but some control signals can be transferred simultaneously via parallel paths 13 1 Keywords for web pages 13 2 Literature EIA plate ELISA sandwich assay enzyme immunoassay separation technique s liquid handling sandwich assay microplate solid phase assays microplate washing Thermo Scientific Microtiter plate Thermo Fisher Scientific microwell plate washing Newman D J and C P Price 1997 Separation Techniques In Principles and Practice of Immunoassay 2 ed eds Price C P and Newman D J pp 154 172 Stockton Press New York Coupe N B and A M Cain Microplate washing the most important step in the ELISA process Labsystems Denley application note Beumer T Stoffelen E Smits J and Carpay W 1992 Microplate washing process description and improvements J Immunol Methods 154 77 87 Parry J V Mortimer P P Friederich P and Connell J A 1997 Faulty washers and soiled micropipettors may generate false positive serological results Clinical and Diagnostic Virology 7 173 181 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 4 Cat no 1507340 63 Appendix A Wellwash 4 Mk 2 Quick Reference Guide Fill the wash bottle and empty the waste bottle Plug th

1507340_ver2.4 Wellwash 4 Mk 2 User Manual

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