WPA S1200 Spectrawave User Manual
Contents
1. Option on display or action Press Comment Make a measurement F2 Select a method F3 Ay Select method number New F1 Name is highlighted Change Name F4 then lt v Enter first character of name then lt Y Enter second character of name gt then lt v Repeat as necessary Accept F4 A is highlighted Change Wavelength F4 then lt v Accept F4 Units is highlighted Select Units F4 then lt 7 Accept F4 Cal is highlighted Select Calibration option F4 then 4 gt Select Factor Enter Factor F4 then Y EA F3 Moves decimal point Positive or negative F2 Alternates between the two Accept F4 Kinetics Leave as no All OK F1 Accept method protocol Run method Fl Insert reference R Used for subsequent samples until RT on display changed Insert sample T Concentration is displayed Repeat as necessary To delete F3 then F1 To exit ESC Note Itis not necessary to enter the name and this can be omitted for a quick measurement Issue 02 03 2005 WPA S1200 English Standard Curve The construction of a multi point calibration curve from standards of known concentration in order to quantify unknown samples is a fundamental use of a spectrophotometer this instrument has the advantage of being able to store this curve as a method using up to 5 standards To include a zero concentration standard include this in the number of standards to be entered and en2. WPA S1200 Spectrawave User Manual Deutsch Espanol 33 WW PA Italiano o iochrom Biochrom Ltd Certificate No 890333 Declaration of Conformity This is to certify that the WPA Spectrawave S1200 and 1200T Visible Spectrophotometer Part number 80 3003 58 and 80 3003 59 Serial number 88000 onwards manufactured by Biochrom Ltd conform to the requirements of the following Directives 73 23 EEC amp 89 336 EEC Standards to which conformity is declared EN 61 010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use EN 61326 1998 Electrical equipment for measurement control and laboratory use EMC requirements Signed Dated 26 Oct 2004 Daus David Parr Managing Director Biochrom Ltd Postal address Telephone Telefax Biochrom Ltd 44 1223 423723 44 1223 420164 22 Cambridge Science Park Milton Road e mail enquiries biochrom co uk website http www biochrom co uk Cambridge CB4 OFJ England Registered in England No 3526954 Registered Office 22 Cambridge Science Park Milton Road Cambridge CB4 OFJ England CONTENTS Unpacking Positioning and Installation Essential Safety Notes OPERATION Introduction Using the Instrument Sample handling tips Absorbance and Transmission Absorbance Ratio Cell Density Proteins Scan Factor Concentration Standard Curve Kinetics To recall a saved method SET UP ACCESSORIES ERROR MESSAGES OUTPUT
3. OF RESULTS Use with serial printer Use with chart recorder USE WITH PC AND THE GRAFICO PC UTILITY SOFTWARE Installation Introduction Menu Descriptions Practical Aspects MAINTENANCE After Sales Support Lamp Replacement Cleaning and general care of the instrument Changing cell holder or removal for cleaning Changing the brightness of the display SPECIFICATION AND WARRANTY NN NOHO PWNAUNUAKRKRWNHN NH 0 SN AK A 15 15 15 16 17 18 18 19 19 20 Unpacking Positioning and Installation e Inspect the instrument for any signs of damage caused in transit If any damage is discovered inform your supplier immediately Check the position of the metal lamp bracket inside the lamp access area e Ensure your proposed installation site conforms to the environmental conditions for safe operation Indoor use only Temperature 5 C to 35 C Note that if you use the instrument in a room subject to extremes of temperature change during the day it may be necessary to recalibrate by switching off and then on again once thermal equilibrium has been established 2 3 hours Maximum relative humidity of 80 up to 31 C decreasing linearly to 50 at 40 C e The instrument must be placed on a hard flat bench or table that can take its weight lt 2 kg such that air is allowed to circulate freely around the instrument e This equipment must be connected to the power supply with the power cord supplied It c
4. R Used for subsequent samples until RT on display changed Insert sample T Abs values displayed for each time interval At end of run calculated AA min correlation coefficient and concentration are displayed To view data Use Page Up and Page Dn F2 or F3 To view graph Fl ESC Return to values Repeat as necessary To exit ESC The Fixed time option is for a single time measurement after a specified time and therefore no options for start time time interval and graphics are available e Ifthe instrument is connected to a chart recorder the output is linearly fitted between data points as the software automatically interpolates these for the benefit of presentation e Ifyou have a factory fitted electrical heated cell holder version of the instrument go to Set up to switch this facility on 37 C Allow 10 minutes for the instrument to come to thermal equilibrium To recall a saved method Option on display or action Press Comment Make a measurement F2 Select a method F3 AY Select method number Accept F4 Selected method is recalled Run method F1 Insert reference R Used for subsequent samples until RT on display changed Insert sample T Issue 02 03 2005 WPA S1200 English 12 SET UP Option on display or action Press Comment Set up instrument F3 Set Language Fl
5. Select display language aw English French German Spanish Italian Accept Fl To exit ESC Comms Software Update F2 Fl Select Communications Select serial printer or PC Fl Alternates between them with default settings for each option Printer 1 is S1000P Printer 2 is Martell Seiko DPU 414 PC is for download to spreadsheet software and Grafico Set Baud of 9600 or 38400 F2 Use 9600 for Grafico and 38400 for download to spreadsheet software Auto print F3 Use only if printer connected select on for automatic increment of sample number and print out after measurement Not recommended for standard curve Do not use in PC mode as output is automatic anyway Accept F4 To exit ESC Set Date Time F3 Select format F1 European or North American Enter values Cras Enter as appropriate Accept F4 To exit ESC Heater control F4 May not be available Heated cell lt Select on for thermostatting at 37 C Accept F4 To exit ESC Heated cell holder factory fitted version only This option cannot be fitted retrospectively to an instrument Issue 02 03 2005 WPA S1200 English 13 ACCESSORIES PC serial cable spare 80 3001 00 S1000P serial printer includes serial printer cable 80 3002 53 Seiko DPU 414 printer 80 2108 80 Serial cable for Seiko printer 80 2118 18 Chart recorder interface cable 80 3003 55 Test tube adapters 10 12 16mm 80 2117 47 ERROR MESSAGES After switch on the instrument
6. and at the rear of instrument 3 Place the instrument on its back insert a small flat headed screwdriver into the potentiometer slot and turn it right or left until a suitable level of brightness is obtained Issue 02 03 2005 WPA S1200 English 19 SPECIFICATION AND WARRANTY Wavelength range 330 800 nm Monochromator Flat grating Wavelength calibration Automatic upon switch on Spectral bandwidth 7 nm Wavelength accuracy 2nm Wavelength reproducibility Inm Light sources Pulsed Tungsten halogen Detector Diode array Photometric range 0 300 to 2 500A 0 3 to 199 T Photometric linearity 2 0 or 0 010A to 1 000A at 546nm whichever is the greater Photometric reproducibility lt 0 002 A at OA and 500nm Stray Light lt 1 T 340nm according to ANSI ASTM E387 72 Stability 0 005A h at OA and 546nm after warm up Noise 0 002A near OA and 0 020A near 2A at 600nm Analogue output 1V per 1 Abs 10 1V 0A offset 1V per 100 T 10 OV 0 T offset Digital output 9 pin serial Dimensions 180 x 270 x 390 mm Weight 1 75 kg Power input 90 265 V 50 60 Hz 15 VA Safety standard EN61010 1 EMC emissions EN 61326 2 3 Generic emissions EMC immunity EN 61000 4 6 Generic immunity part 1 Mains harmonics EN 61000 3 2 Susceptibility standard IEC 801 Quality System Designed and manufactured in ac
7. can add the time in 10 second intervals to the spreadsheet manually note that first data point is after 10 seconds not zero seconds and then graph the absorbance time data see scan mode export to excel for more details Scan mode Files can be saved as txt csv opens directly in Excel when double clicked or wmf picture formats Label a peak by dragging and releasing the icon at the left side of the graph The absorbance wavelength details are shown in the title bar Dragging it again moves the label moving it the left hand side takes the label away Multiple peaks can be added Use display grid off for clearer presentation Data can be output in absorbance only Scan mode export to Excel and graphing If saving as a txt file save the results to folder of choice o Use Excel to open this file with files of type set to all files o Note that saving as a csv file and double clicking on it will open Excel directly Highlight the wavelength and absorbance values and click the graph icon Select chart type X Y Scatter and the curved lines no data points option Label the axes etc as required Double click on the x axis select Scale and minimum to 330 and maximum to 800 Set colour scheme to suit your preferences Issue 02 03 2005 WPA S1200 English 17 MAINTENANCE After Sales Support Support agreements that help you to fulfil the demands of regulatory guidelines concerning GLP GMP are availabl
8. with the shield connecting the black plug and pin 5 and the core connecting the red plug and pin 1 Issue 02 03 2005 WPA S1200 English 14 USE WITH PC AND THE GRAFICO PC UTILITY SOFTWARE Your instrument is supplied with a serial lead and Grafico software on the user manuals CD that enables it to be connected to a PC so that results can be captured stored printed and transferred into other applications easily In particular a complete wavelength scan can be visualised on the PC and copied pasted into a word document or powerpoint presentation An informative tutorial on aspects of UV Visible Spectrophotometry is available as part of the software Installation Prior to installation of Grafico you should have the following options selected in your spectrophotometer instrument set up PC 9600 Baud Autoprint off The software takes up approximately 0 5Mb of hard disk space when installed Proceed as follows to install the software 1 Place CD into the CD drive of the PC 2 Use Windows explorer to locate the setup exe file Grafico folder within the appropriately named instrument folder on the user manuals CD 3 Double click on this so that the software installs filling out the information as requested 4 The software can be started directly by Start gt Programs gt Grafico Introduction e When Grafico is selected you are prompted to enter the file details note that the title entered here is used as the title of the wa
9. 0 English 9 gt F4 All OK F4 Accept Standards Change Curve Fit algorithm F3 then Y F4 Select linear least squares or polynomial curve View Graph F4 Accept graph F3 Can now run samples To run samples All OK Fl Run Fl Insert reference R Used for subsequent samples until RT on display changed Insert sample T Concentration is displayed Repeat as necessary To delete F3 then F1 To exit ESC To delete F2 then F1 To exit ESC Ensure Autoprint in set up is set to off Issue 02 03 2005 WPA S1200 English 10 Kinetics Kinetics studies where the change in absorbance needs to be followed as a function of time at a fixed wavelength can be readily performed Reagent test kits are routinely used for the enzymatic determination of compounds in food beverage and clinical laboratories by measuring NAD NADH conversion at 340 nm The change in absorbance over a specified time period can be used to provide useful information when an appropriate factor defined in the reagent kit protocol is applied Reaction rate and enzyme activity can be calculated if the factor used takes account of the absorbance difference per unit time as opposed to the absorbance difference per se For this reason the change in absorbance per minute AA min concentration AA min x factor and correlation coefficient calculated from a best fit of the data points are dis
10. an be used on 90 240V supplies Switch on the instrument via the display after it has been plugged in The instrument performs a series of self diagnostic checks for lamp performance wavelength calibration and diode array pixels press F2 to proceed If the instrument has just been unpacked or has been stored in a cold environment it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before switching on to prevent calibration failure as a result of internal condensation The cell holder supplied with the instrument accepts standard 10mm pathlength glass or plastic cells adapters are available to convert it to accept 10 12 and 16mm diameter test tubes It can be removed for cleaning if spillages occur by undoing the screws that hold it or it can be flushed through with water in situ Ifthe instrument has a heated cell holder option and it is on allow 10 minutes for it to come to thermal equilibrium This cell holder cannot be removed If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation the protection provided by the equipment may be impaired and instrument warranty withdrawn Essential Safety Notes There are a number of warning labels and symbols on your instrument These are there to inform you where potential danger exists or particular caution is required Before commencing installation please take time to familiarise
11. cordance with an ISO 9001 approved quality system Specifications are measured after the instrument has warmed up at a constant ambient temperature and are typical of a production unit As part of our policy of continuous development we reserve the right to alter specifications without notice Warranty Your supplier guarantees that the product supplied has been thoroughly tested to ensure that it meets its published specification The warranty included in the conditions of supply is valid for 12 months only if the product has been used according to the instructions supplied They can accept no liability for loss or damage however caused arising from the faulty or incorrect use of this product This product has been designed and manufactured by Biochrom Ltd 22 Cambridge Science Park Milton Road Cambridge CB4 OFJ UK Issue 02 03 2005 WPA S1200 English 20
12. e Calibration certification using filters traceable to international standards Certificated engineers and calibrated test equipment Approved to ISO 9001 standard Choice of agreement apart from break down coverage can include Preventative maintenance Certification When using calibration standard filters insert such that the flat surface is facing away from the spring end of the cell holder Observe all necessary precautions if dealing with hazardous samples or solvents Lamp Replacement A replacement lamp is available from your supplier using the following part numbers Tungsten Lamp S1000L 80 2115 33 use only this tungsten lamp as it is supplied with the connection wires others will not operate correctly in this spectrophotometer The design of the lamp area is such that users are able to change their own lamps No lamp alignment is necessary as the lamp is pre aligned The lamp becomes hot in use Ensure it is cool before changing it Do not touch the optical surfaces of the lamp with your fingers use tissue if touched the area should be cleaned with iso propanol Instructions for lamp change are provided with the lamp and overleaf Issue 02 03 2005 WPA S1200 English 18 To change the lamp proceed as follows 1 Switch off the instrument remove the sample from the cell holder and disconnect the power supply cord 2 Remove the protective layers at the lamp access and plug in poi
13. e air The procedure is as follows Option on display or action Press Comment Make a measurement F2 Single Multi Ratio Fl Single A Fl Abs T F1 Alternates between the two Set F2 then lt Y Select wavelength Accept F2 Insert reference R Used for subsequent samples until RT on display changed Insert sample T Value is displayed Repeat as necessary To exit ESC To make up to 4 absorbance measurements on the same sample Option on display or action Press Comment Make a measurement F2 Single Multi Ratio F1 Multi A F2 Set A s Fl then lt Y Select first wavelength Select F1 then lt v Select second wavelength Repeat as necessary All OK F4 Insert reference R Used for subsequent samples until RT on display changed Insert sample T Absorbance values are displayed Repeat as necessary To exit ESC Issue 02 03 2005 WPA S1200 English 4 Absorbance Ratio This makes simple absorbance ratio measurements on samples measuring the amount of light that has passed through a sample relative to a blank this can be air at two wavelengths The procedure is as follows Option on display or action Press Comment Make a measurement F2 Single Multi Ratio Fl Ratio F3 Remove this row Set Al F2 then lt v Select wavelength Accept F2 Se
14. es Issue 02 03 2005 WPA S1200 English To run samples All OK Fl Run Fl Insert reference R Used for subsequent samples until RT on display changed Insert sample T Concentration is displayed Repeat as necessary To exit lt Ensure Autoprint in set up is set to off Scan An absorption spectrum can be obtained from your instrument enabling simple identification of peak height and position The procedure is as follows Option on display or action Press Comment Make a measurement F2 Scan F4 Abs T Fl Alternates between the two Insert reference R Used for subsequent samples until RT on display changed Insert sample T Scan is displayed Repeat as necessary To identify peaks Move cross hairs a gt Abs and A values appear at top To zoom in on a region of interest Zoom F2 then Move box that appears on display to area of interest Zoom in F1 Examine detail Zoom out F1 Return to original data To exit ESC Issue 02 03 2005 WPA S1200 English 7 Factor Concentration Factor concentration mode is used when a conversion factor is known and is required to convert the absorbance measurement for a sample at a specific wavelength into a concentration by a simple multiplication of absorbance x factor The procedure to define a new method is as follows
15. file in the file format selected The file details are included with this data Displays a tabbed dialogue box so that automatic post process options for saving of graph printing of graph and the graph scaling parameters can be defined The default data directory can be defined and is used for all save operations Prints the entire file including a header if defined in File gt New Runs the Common Print Dialog function to set up the printer Closes the application Copies the data to clipboard for pasting into another application in data logging and scan modes this is text and graphic respectively Clears the data from the data set Selects data and header together Switches between scan and data logging modes Successive scans overwrite existing scans on the display and can be saved if the autosave function is on Shows the file details entered at the start or after File gt New and allows modification of these details if required Automatically sets the scale of the absorbance axis to optimise presentation 2 5 2 0 1 5 1 0 0 5 0 2 or 0 1A Sets the scale to user preference Full Auto Define Toggles on off the grid on the graph for presentation purposes View menu bar as icons View status bar at bottom of display View tutorial on UV Visible spectrophotometry View help topics View version number etc Issue 02 03 2005 WPA S1200 English 16 Practical Aspects Data logging mode When exporting rate mode results you
16. n or change Use F4 to implement change followed by 4 P to choose between options indicated and Y to enter alphanumeric characters for example in the selection of a wavelength or entry of a method title Then use F4 to accept the change made ESC to escape or stop making measurements R to set reference to 0 000AU or 100 T on a reference solution at the current wavelength in the mode selected or to do a reference scan if in scan mode T to start making measurements Display The following symbols will appear in bottom right hand corner and mean the following Use 4 P lt to select option RT Ready to set reference or run sample Issue 02 03 2005 WPA S1200 English Sample handling tips e Note that the light beam shines from front to back through the cell chamber ensure the cell is inserted in the correct alignment e The optical height is 15mm and the minimum volume that can be used is approx 700ul in a semi micro cell e Align the indicator line on test tubes with the arrow on the cell compartment area to ensure reproducible positioning of the tube Note that test tubes do not last forever and that the surface gets scratches and blemishes through repetitive use if this is the case they should be replaced Absorbance and Transmission This makes simple absorbance measurements on samples measuring the amount of light that has passed through a sample relative to a blank this can b
17. nts on the underneath of the instrument 3 Remove the lamp wires from the groove by gently unclipping it 4 Remove the lamp by twisting the lamp assembly anti clockwise 5 Remove the lamp connection end by gently pulling with your fingers 6 Replace with new lamp using the reverse of these actions Cleaning and general care of the instrument External cleaning Switch off the instrument and disconnect the power cord Use a soft damp cloth Clean all external surfaces A mild liquid detergent may be used to remove stubborn marks Changing cell holder or removal for cleaning e Undo the screws that are visible on the top of the cell holder using a small flat headed screwdriver and lift the holder out by holding onto the projection this may require pushing to the right as you do so in order to prevent fouling against the left side of the instrument cover If necessary the cell holder can be helped out by pushing from the bottom of the instrument e Insert the test tube holder and secure in place using the same screws e Note that as well removal for cleaning spillages in the cell holder can be flushed through using water from a squeeze bottle in order to prevent crystallisation fermentation of residues Changing the brightness of the display To change display brightness proceed as follows 1 Ensure the instrument is on and that there is no sample in the cell holder Remove the protective layer at the lamp plug in point underneath
18. played They may not be relevant for simple kinetics experiments The procedure to define a new method is as follows Option on display or action Press Comment Make a measurement F2 Select a method F3 An Select method number New F1 Name is highlighted Change Name F4 then lt v Enter first character of name gt then lt Y Enter second character of name gt then lt v Repeat as necessary Accept F4 Wavelength is highlighted Change Wavelength F4 then lt v Accept F4 Units is highlighted Enter Units F4 then 7 Accept F4 Cal is highlighted Select Calibration option F4 then 4 gt Select Factor Enter Factor F4 then lt v If required this is used to convert AA min to Concentration eris F3 Moves decimal point Positive or negative F2 Alternates between the two Accept F4 Kinetics F4 then lt P Select Yes or Fixed time Accept F4 Start is highlighted Enter Start Time F4 then Usually 00m 00s unless there is a lag R time Accept F4 Interval is highlighted Enter time interval between F4 then Minimum interval is 10 seconds each measurement bay Issue 02 03 2005 WPA S1200 English 11 Accept F4 End is highlighted Enter end time F4 then Maximum time is 59m 59s after bay completion of start time Maximum number of readings is 20 sO maximum end time is 20 x the time interval Accept F4 Run method Fl Insert reference
19. r hardcopy output or to a PC for download of results to spreadsheet link via a converter lead to chart recorder set the date for print outs The instrument is supplied with Grafico PC utility on the accompanying CD and a serial lead These provide the user with the means to capture print and store data from the instrument to a PC Specifically it e produces a printable graphical plot of the scan in Abs e logs date time and serial number with any output from the instrument e produces a results log in order to store tabulate and subsequently print output from the instrument e enables export of the output from the instrument to Excel as a text file A tutorial on UV Visible spectrophotometry is included as part of the Grafico software Issue 02 03 2005 WPA S1200 English 2 Using the Instrument The back lit liquid crystal display is very easy to navigate around using the function select and arrow keys on the hard wearing spill proof membrane keypad sep pete Le canner SIDO0 Difer Atay Spec trapehatrnneter bioghrom Keypad F1 F2 F3 F4 The function select entry soft keys on the keypad are situated next to the corresponding option on the display and are used to select an appropriate mode 4 ha When a parameter within a mode needs selecting or changing as indicated by on the display the four arrow keys 4 gt lt v are used in conjunction with the function keys to make that selectio
20. s Comment Make a measurement F2 Cell Density Proteins F2 Proteins F2 Select Protein method F1 F2 F3 or F4 BCA Bradford Lowry or Buiret protocol can be changed to suit Enter Dilution if applicable F2 then F4 moves decimal point Program method Fl Select Units Fl 7 Accept F4 v Cal is highlighted Select Calibration option Fl 4 gt F4 Select Std Enter number of Replicates ay 1 2 or3 Accept F4 Goes to Calibration Curve page Set Std F1 1 is highlighted maximum is 5 Change F1 then lt v Enter concentration of standard 1 states F3 Moves decimal point Accept F4 2 is highlighted Change Fl then lt Y Enter concentration of standard 2 F3 Moves decimal point Repeat as necessary Incorrect entry F3 then F1 Clears entry prior to re entry FA Use F3 to clear standard from experiment Standards are all OK F4 Accept Concentrations Insert reference R Used for subsequent samples until RT on display changed Insert Standard 1 T Absorbance for Std 1 is measured If replicates selected gt T Absorbance for Std 1 Rep 2 is measured A Insert Standard 2 YTI Absorbance for Std 2 is measured Repeat as necessary Incorrect entry F3 then F1 Clears entry prior to re entry ax F4 All OK F4 Accept Standards Change Curve Fit algorithm F3 then 7 F4 Select linear least squares or polynomial curve View Graph F4 Accept graph F3 Can now run sampl
21. t Al F2 then lt v Select wavelength Accept F2 Insert reference R Used for subsequent samples until RT on display changed Insert sample T Ratio is displayed Repeat as necessary To exit ESC Cell Density This function should be used to make an OD600nm reading on a cell culture rather than a direct absorbance reading as it compensates for turbidity using an auto correction at 800nm The absorbance at two wavelengths is measured simultaneously and an algorithm applied to compensate for the scattered light Different instruments give different OD600 due to differences in the optical systems so a conversion factor may be required for direct comparison We recommend the use of disposable cells rather than test tubes for this application The procedure is as follows Option on display or action Press Comment Make a measurement F2 Cell Density Proteins F2 Insert reference R Used for subsequent samples until RT on display changed Insert sample T Value is displayed an auto correction factor is applied to the Absorbance value Repeat as necessary To exit ESC Issue 02 03 2005 WPA S1200 English 5 Proteins The proteins function should be used to for the standard protein determinations BCA at 562nm Bradford at 595nm Lowry at 750nm Biuret at 546nm The BCA Bradford Lowry and Biuret methods are based on a standard curve routine Option on display or action Pres
22. ter 0 00 for concentration use a blank when required to enter standard The procedure to define a new method is as follows Option on display or action Press Comment Make a measurement F2 Select a method F3 AT Select method number New F1 Name is highlighted Change Name F4 then lt v Enter first character of name then lt Y Enter second character of name gt then lt v Repeat as necessary Accept F4 is highlighted Change Wavelength F4 then v Accept F4 Units is highlighted Select Units F4 then lt v Accept F4 v Cal is highlighted Select Calibration option F4 then 4 gt Select Std Accept F4 Goes to Calibration Curve page Set Std F1 1 is highlighted maximum is 5 Change F1 then 2 v Enter concentration of standard 1 see F3 Moves decimal point Accept F4 2 is highlighted Change F1 then lt Y Enter concentration of standard 2 F3 Moves decimal point Repeat as necessary Incorrect entry F3 then F1 Clears entry prior to re entry lt Y F4 Standards are all OK F4 Accept Concentrations Insert reference R Used for subsequent samples until RT on display changed Insert Standard 1 T Absorbance for Std 1 is measured Std 2 is highlighted Insert Standard 2 T Absorbance for Std 2 is measured Std 3 is highlighted Repeat as necessary Incorrect entry F3 then F1 Clears entry prior to re entry Issue 02 03 2005 WPA S120
23. undergoes self diagnostic tests for the tungsten lamp wavelength calibration and diode array as part of its calibration procedure v for OK X for fail The results of this test are displayed and can be printed out or output to PC for filing and GLP Good Laboratory Practice purpose The messages for tungsten lamp and wavelength calibration are self explanatory involving checking that the cell compartment is clear or replacement of the tungsten lamp In the unlikely event of a diode array fail message contact your local supplier OUTPUT OF RESULTS Use with serial printer Note that all results can be output to PC using the serial lead and Grafico software supplied on the user manuals CD Seiko DPU 414 settings Dip SW 1 Serial Auto line feed off Dip SW 2 40 column width International character set USA Dip SW 3 Baud rate 9600 bps Note that the 80 2108 18 lead that is required will need two small nuts removing before connection Use with chart recorder Kinetics results can be output to a chart recorder using the appropriate cable 80 3003 55 Voltage setting is 1V per 1 Absorbance unit 10 with an offset of 1V 0 000 Abs on the chart recorder corresponding T values are 1V per 100 T 10 with OV 0 T To make the chart cable yourself you require a female 9 way D type at one end with two 1 red 1 black 4mm banana plugs at the other depending on the chart recorder and 2 metres of coaxial cable or screened twin core
24. velength scan graph After pressing OK the instrument it should be already switched on and connected to the PC with the serial lead is recognised by the software e There are two parts to the Grafico software data logging and scan e The default mode is data logging this receives instrument output from absorbance T concentration and rate measurements including time and date stamp o Results can be copied from Grafico and pasted directly into Excel for ease of data transfer Alternatively results can be saved and opened up using Excel e If scan mode is selected View gt Scan mode the full 330 800nm wavelength scan output from the instrument is shown just press the run key as usual Multiple peaks can be identified using a trace routine and labelled if required by dragging the icon at the left side of the displayed graph and releasing at the appropriate point o Graphs can be copied and pasted into Word Excel or powerpoint o Graphs can be saved in a format that can be opened directly by Excel Issue 02 03 2005 WPA S1200 English 15 Menu Descriptions File New Save Save As Setup Print Print Setup Exit Edit Copy Clear Select All View Scan mode File details Autoscale Set scale Display grid Toolbar Status bar Help Tutorial Help topics About Clears any existing data and starts a new report Prompts for file details user name organisation title descriptive text Saves the data
25. yourself with these symbols and their meaning Caution refer to accompanying documents Background colour yellow symbol and outline black Issue 02 03 2005 WPA S1200 English 1 OPERATION Introduction Your spectrophotometer is a simple to use microprocessor controlled instrument It is a diode array product 1024 pixels has no moving parts and scans very quickly After switch on calibration and pressing F2 to proceed the home page is shown offering the choice of Repeat last operation Make a measurement Set up instrument Repeat last operation returns the user to the last screen displayed when the instrument was switched off and provides a short cut to the last test that was performed Within Make a measurement your spectrophotometer has facilities for measurement of absorbance transmission ratio and concentration values cell culture optical density measurements at 600nm entry of a multi point standard curve in memory output of wavelength scan to display output of kinetics assay to display application of a factor to an absorbance change over a specified time interval for an enzymatic determination reaction rate storage of up to 99 user defined methods Within Set up instrument your spectrophotometer can be set up to select the display language option English French German Spanish Italian link via a serial lead to either a serial printer fo
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