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Biomek NX Multichannel Hardware Manual.book

1. Figure 2 7 Chamfer location on a Multichannel head 7 Slide the first shoulder screw Shoulder 1 into the head Figure 2 6 Make sure the shoulder screw is in far enough to engage the threads Figure 2 8 Sequence for installing four plunger screws and four shoulder screws on all multichannel heads Beckman Coulter Inc Multichannel Pod 2 11 8 Using a 9 64 Allen wrench tighten the first shoulder screw until it is snug then back it off loosen it 1 2 turn Note This 1 2 turn allows the other three shoulder screws to be installed without skewing the head 9 Using the sequence designated in Figure 2 8 follow the procedure in step 6 to install the remaining three shoulder screws 10 After all four shoulder screws are installed go back and tighten them securely in the same order 11 Using a 9 64 Allen wrench follow the installation sequence designated in Figure 34 8 to install the four plunger screws Turn each plunger screw until barely snug 12 After all four plunger screws are installed go back and tighten all of them securely in the same order 13 Replace the bottom pod cover and tighten the three screws 14 Turn on the power 15 Retract the grippers using Advanced Manual Control Note After installing a multichannel head on a Multichannel Pod Hardware Setup must be updated refer to Section 3 3 Configuring a Multi
2. Choose from Shift deck Shift ALP or Shift position for appropriate teaching instructions refer to Section 4 2 4 1 Selecting Appropriate Teaching Instructions Ifthe coordinates displayed appear reasonable choose OK Position Properties appears again and the position is framed for the selected pod Choose OK to close Position Properties For framing additional positions move the AccuFrame to the next position to frame refer to Section 4 2 3 Installing AccuF rame and repeat steps 3 through 11 Choose Save to save framing information for all positions and close the Deck Editor Figure 4 4 Note Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened Remove the framing fixture from the head of the Multichannel Pod Biomek NX Multichannel Liquid Handler User s Manual 4 10 Framing Instructions 4 2 4 1 Selecting Appropriate Teaching Instructions In Teaching Instructions Figure 4 7 the entire deck a deck position or an ALP can be shifted by the teaching process Determine what should be shifted using the following information taught Shift deck shifts all ALPs and positions resulting in incorrect CAUTION Use Shift deck only before other ALPs or positions are coordinates if applied to previously framed ALPs and positions e Shift deck shifts all ALPs and positions associated with the deck by the change amounts shown
3. COULTER Biomek NX Multichannel Liquid Handler Hardware User s Manual Beckman Coulter PN 987892 Revision AA January 2004 Beckman Coulter Inc 4300 N Harbor Boulevard Fullerton CA 92834 3100 Copyright 2004 Beckman Coulter Inc Printed in U S A Except as provided in writing signed by an officer to Beckman Coulter Inc this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but SAGIAN does not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability currentness omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user Except as expressly provided herein SAGIAN makes no other warranty whether oral or written expressed or implied as to any matter whatsoever including but not limited to those concerning merchantability and fitness for a particular purpose nor is freedom from any patent owned by SAGIAN or by others to be inferred LIMITATIONS OF LIABILITY SAGIAN shall not be liable to any extent whatsoever for any damages resulting from or arising out of the use or performance of this system and related documentation or the procedures specified in this man
4. 12 Choose Advanced Controls and select Pod1 Advanced Manual Control appears Figure 4 21 13 Choose Move Z Max Note Choosing Move Z Max raises the pod to allow access to the AccuFrame 14 Choose Close to close Advanced Manual Control 15 Choose Exit to close Manual Control 16 Remove the AccuFrame from the ALP position 4 4 1 Testing Gripper Framing Accuracy To make sure the grippers work properly create and run a method at reduced speed around 10 to move a piece of labware from one framed deck position to another refer to Section 3 3 2 Setting Multichannel Pod Properties If problems occur repeat the gripper framing process until the grippers are working correctly Beckman Coulter Inc Framing Instructions 4 27 4 5 Troubleshooting Perform the troubleshooting techniques provided in Table 4 1 when necessary Note In the case of any other framing related problems contact a Beckman Coulter Service Engineer Table 4 1 Troubleshooting Framing If Then AccuFrame power light not on Check the CAN connection to make sure the AccuFrame is connected to Biomek NX instrument The Y axis and the X Z axes Light Make sure the AccuFrame is Beams cannot be broken when receiving power moving a finger around the interior of AccuFrame One or both beam indicator lights on There is most likely an internal AccuFrame stay on when no objects obstruction to sensors Call a are breaking the light bea
5. ssessssessssessesssssresseserssrsseesse 4 4 Fig re 4 3 Framing FIXtUT sa vvaprcnacvarsesesnoncesasnedveaswwat ae oaia EN ENARE EE EREE EKETA EAEE EESE 4 5 Biomek NX Multichannel Liquid Handler User s Manual xvi List of Figures Figure 4 4 Deck Editot sac52asssssidacssancsnensdvaseasvacssessiadsonssGayhdatvianiniesssaaniediaassald sap RANEA EEA E EEEE 4 7 Fig re 4 5 Position Properties siasscsernivecsacenacesuvneieunsmarsnuhendoeasannyesielacsvosnertsnaatanntsseplaasidontconts 4 8 Fig r TATUM occ se coro ce ng arene hag ec ex unpeaGe uaachan ona a Aeae AE AAEN EEEa EE AEA Rena ia antaG iee 4 8 Figure 4 7 Teaching TS CUO serisicsseia nsise ieni EEA E DE EENES 4 9 Figure 4 8 Deck Editor amp ycicceraceancensetenas ceyunuaterasencsvediecwus derine arent resusitet Ereet SERRE Tantana iNES ie Staate reS 4 11 Figure 4 9 Position Properties for a Positive Position ALP sssessessessssesessrseesesseserssssrrsrssesee 4 12 Figure 4 10 Manual Framing Wizard Warning ccccccsccesceesceeseeceseceeceeceeeaeecsaeceeenaes 4 12 Figure 4 11 Manual Framing Wizard if tips are not already loaded eee eeceseeeeeeeeeeeeeee 4 13 Figure 4 12 Manual Framing Wizard if tips are loaded cccecseceseceseceeeeeeeeeeeeceeeeeseeesaeenes 4 13 Figure 4 13 Framing to the corner of four Wells cssccssesssoncessestcerscrscescceetssensonsenscoacenacsenes 4 15 Figure 4 14 Framing to the center of a well nec csatssaceicdessdesmas
6. The Biomek NX Multichannel liquid handler is controlled using Biomek Software operated from the host computer The Biomek NX Multichannel can be e Operated as a standalone instrument gripping and moving microplates without the assistance of a laboratory robot using the gripper tool e Integrated into an automated robotic system e Operated using a combination of these capabilities Biomek NX Multichannel Liquid Handler User s Manual 1 2 Biomek NX Multichannel Instrument Introducti 1 2 System Components The system components described below correspond to the configurations in Figure 1 1 Multichannel Pod with gripper tool Figure 1 1 Biomek NX with Multichannel Pod Beckman Coulter Inc iomek NX Multichannel Instrument Introduction 1 3 1 2 1 Towers The Biomek NX Multichannel towers Figure 1 1 form the two upright sections of the base unit along which the pod travels on the X axis The links for master control of the Biomek NX system plus utility hook ups and ALP connections are located on the towers Green and amber indicator lights are built into the main cover and keep users aware of the current operational status of the liquid handler The towers house the following connections Figure 1 2 Figure 1 3 and Figure 1 4 e Main power switch on the outside of the right tower e Communication hookup to host computer via RS232 on the outside of the right
7. A Beckman Coulter Service Engineer frames the grippers during system installation however it may be necessary to repeat the framing procedure if e the head is changed e grippers are replaced e extraordinary circumstances occur such as accidentally bending a gripper Before framing the grippers make sure instrument is homed refer to Section 4 2 1 Homing All Axes of the Pods e deck position is framed prior to framing the grippers to that location refer to Section 4 2 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame e AccuFrame is installed on the appropriate position refer to Section 4 2 3 Installing AccuFrame e framing fixture is not attached to the Multichannel Pod To frame the grippers 1 Choose Start gt Programs gt SAGIAN Software gt Biomek Software to start the Biomek Software 2 From the Instrument menu choose Hardware Setup Hardware Setup appears 3 Choose the Multichannel Pod The configuration to frame the grippers appears on the right Figure 4 18 Reconnect ff Home All Axes db Add Device s Ren x Accept gt lt Cancel Sl Bi NX SN N ad H m A ara one Serial Number None Save Settings Restore Settings Delete Settings 96 Head Type 36 Mandrel 200 pL Head x Last Validation Not Specified Set Validation Time 1 amp Devices Axis Limit Settings 2 ea X em Y cm Z cm D ul D em Correlate Pads 67 Shaker a
8. NX Multichannel Liquid Handler User s Manual 3 10 Configuring the Biomek NX MC in Biomek lt 3 4 Saving Restoring and Deleting Settings The axes limits and pod settings may be saved restored and deleted using the Save Settings Restore Settings and Delete Settings options in Hardware Setup These options are particularly useful when changing heads on a Multichannel Pod Because a new D axis limit must be established when a head is changed these options allow the D axis limit to be saved and restored for specific head types When settings are no longer needed they may be deleted 3 4 1 Saving Pod Settings To save settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane 2 Choose Save Settings Save Configuration appears Figure 3 6 Save Configuration Name M ultichannelPodSettings1 Description Figure 3 6 Save Configuration 3 Create a name for the current configuration 4 If desired enter a description of the configuration 5 Choose OK The settings are saved Beckman Coulter Inc ing the Biomek NX MC in Biomek Software 3 11 3 4 2 Loading Pod Settings To load the saved settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane 2 Choose Restore Settings Load Settings appears Figure 3 7 Load Settings Settings Sett
9. Orient the gripper so that the front of the gripper faces the outside and gripper pads face the inside of the Multichannel Pod Figure 2 10 Grasping both sides of the gripper push the gripper straight up into the Multichannel Pod Note A click can be heard and felt when the grippers are seated in the Multichannel Pod Pull down on the gripper to ensure that it is fully seated in the pod Reframe the Multichannel Pod refer to Section 4 4 Framing the Grippers Configuring the Biomek NX MC in Biomek Software 3 1 Overview The Biomek NX Multichannel liquid handler must be configured in Biomek Software to ensure proper operation Hardware Setup tells Biomek Software what devices pods and heads to expect on the instrument by providing a connection between the instrument and the software This connection is established by installing configuring and removing devices in Hardware Setup Any active ALPs that require a CAN communication or other devices integrated on the deck of the Biomek NX Multichannel must also be configured in Hardware Setup Refer to the ALPs User s Manual or the specific device integration manual for instructions on configuring the ALP or device in Hardware Setup Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support f CAUTION Do not make any changes to the pod axes limits in Hardware Hardware Setup is used for e Configuring the Biomek
10. NX Multichannel Instrument Section 3 2 Configuring a Multichannel Pod Section 3 3 Saving Restoring and Deleting Settings Section 3 4 Biomek NX Multichannel Liquid Handler User s Manual 3 2 Configuring the Biomek NX MC in Biomek 3 1 1 Accessing Hardware Setup Hardware Setup Figure 3 1 is accessed from within Biomek Software To access Hardware Setup 1 Choose Start gt Programs gt SAGIAN Software gt Biomek Biomek Software appears 2 From the Instrument menu choose Hardware Setup Hardware Setup appears Figure 3 1 Biomek Hardware Setup 2 Reconnect Mf Home All Axes dp Add Device ms Remove Device Y Accept gt lt Cancel iomek nx SN None Serial mmber o fa AccuFrame 96 Podi Port Simulate gt a Devices 7 DeviceController0 Pod Type Multichannel Pod 7 7 ShakerALPO g7 ShakerALP1 oF StirrerALPO oF StirrerALP1 67 TiploaderO 47 Tiploader1 f Digital Devices ep Simulator E Camera E Materials amp Stacker Carousels BiomekNX MC _ Figure 3 1 Hardware Setup for a Biomek NX Multichannel instrument Beckman Coulter Inc ifiguring the Biomek NX MC in Biomek Software 3 3 3 1 2 Understanding the Options in Hardware Setup An understanding of the options on the toolbar in Hardware Setup Figure 23 1 is necessary to properly install configure and remove devices Table 23 1 lists and describes the toolbar opt
11. Setup showing the configuration of a Multichannel Pod A CAUTION Always ensure that the grippers are retracted before homing 4 Choose Home All Axes Choosing Home All Axes homes all pod axes The following Warning appears Figure 3 3 Warni aI xl CAUTION Before selecting OK to home all axes the Following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Cancel Figure 3 3 Warnings must be addressed before homing process begins 5 Choose OK after confirming that the Warning has been addressed appropriately Note Additional Warnings may appear depending on deck configuration and the type of head installed on the Biomek NX Multichannel instrument Address all warnings appropriately and choose OK to continue 6 In Hardware Setup choose Set D to establish a new D axis limit Beckman Coulter Inc ing the Biomek NX MC in Biomek Software 3 7 7 Choose Set Z to establish a new Z axis limit 8 Frame the grippers according to the procedures outlined in Section 4 4 Framing the Grippers Note An asterisk next to the pod indicates that the head has been modified since the workspace was loaded Note Change Head is used only when physically removing or installing a head Choosing Change Head mov
12. collisions is not completely eliminated by this command for example if the gripper is extended and holding a microplate and the pod is moved over a tip box on a tiploader a collision could occur 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 Choose Advanced Controls Select Pod1 Advanced Manual Control for the pod appears Figure 5 5 Choose Move Z Max Choose Close to close Advanced Manual Control nH Wn A W N Choose Exit to close Manual Control Controlling the Biomek NX in Biomek Software 5 9 5 4 3 Understanding Movement Vectors for the Pod The use of Advanced Manual Control for the pod centers around the building and applying of movement vectors A movement vector simply indicates the magnitude and direction of motion applied to the pod When the point of reference is at the front and center of the unit positive and negative pod values are as follows e Positive value X right motion e Positive value Y forward motion e Positive value Z up motion e Positive value D squeezing aspirating motion e Negative value X left motion e Negative value Y back motion e Negative value Z down motion e Negative value D unsqueezing dispensing motion 5 4 4 Setting Delta Values for the Pod A Delta value is the amount of change in an axis that is applied to the Movement Vector when a button in the Vector Builder is pressed For example if the Delta value for X
13. grippers Biomek NX Multichannel Liquid Handler User s Manual 4 24 Framing Instructions 7 Choose OK This moves the pod to that position and extends the grippers Advanced Manual Control Figure 4 21 and Frame Gripper Figure 4 22 appear side by side Note The grippers are extended a little high and opened a little wide at the end of the move initiated by step 6 Advanced Manual Control Podi Multichannel Vector Builder Delta Home D A nea x o cm Home zZ xY a7 a Extend Gripper Left eo gt Right zZ 0 1 cm 0 1 Retract Gripper Fwd i D cm x Down Move Z Max D Units sie lt gt C om Absolute Move Squeeze Aspirate UnSqueeze Dispense T pl Current Position x o cm Y 0102 om Z 3 95 cm D 0 342 cm Movement Vector x fo cm Y fo cm Z fo cm D fo cm Stop MV utoClear 7 Clear Speed 100 rA Go S Figure 4 21 Advanced Manual Control for moving grippers during framing Note Do not select Close if Advanced Manual Control closes the framing process must be completed again beginning with step 1 Manually move the pod into gripping position and hit OK Cancel Figure 4 22 Moving pod into gripping position Beckman Coulter Inc Framing Instructions 4 25 8 Use Advanced Manual Control to move the grippers until they are aligned with the bottom of the AccuFrame Figure 4 2
14. pod Due to the weight of the head approximately five pounds maintain a firm grasp on the head while sliding the head out 5 Cover and store the head flat side down in a clean cool dry place 2 3 1 2 Installing Multichannel Heads CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist ground strap when installing a multichannel head Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek NX instrument The same method is used to install the 96 Channel 20 uL Head 96 Channel 200 uL Head and 384 Channel 30 uL Head To install a head 1 In Hardware Setup select the appropriate Multichannel Pod Note A Multichannel Pod is identified with a 96 or 384 2 Choose Change Head Note Choosing Change Head moves the D axis to 0 6 cm and extends the grippers 3 Turn off power and make sure the plastic bottom cover is removed Figure 2 4 Note Do not remove top cover 4 Position the strap portion of the wrist ground strap snugly around the wrist Figure 2 3 Biomek NX Multichannel Liquid Handler User s Manual 2 10 Multichannel Pod 5 Clamp the alligator clip of the wrist ground strap to the instrument deck base plate Figure 2 3 6 With the chamfers Figure 2 7 pointed to the back of the instrument carefully slide the head up into the pod Geo o Oe
15. strap when installing a multichannel head WARNING Grippers may bend if not taught framed properly with the AccuFrame WARNING Do not connect or disconnect any cable while power is applied to the Biomek NX WARNING Avoid all labware on the Biomek NX deck when removing or installing grippers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware PEPE EPEEEE PPRP Biomek NX Multichannel Liquid Handler User s Manual x Safety Information PRP EER gt A A A A A A Beckman Coulter Inc CAUTION Use caution when removing grippers Since grippers mount tightly into the Multichannel Pod they pull free of the Multichannel Pod suddenly CAUTION Do not make any changes to the pod axes limits in Hardware Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support CAUTION If the hardware configuration is not updated using Hardware Setup hardware crashes or inaccurate liquid transfer may occur CAUTION To avoid hardware crashes a new D axis limit must be established and the grippers framed in the Biomek Software after the head has been configured in Hardware Setup CAUTION Always ensure that the grippers are retracted before homing CAUTION Before selecting OK to home all axes the following conditions must be met e Make sure there is no liquid present in the tips e Make sure there are no disposa
16. top cover 2 3 1 1 2 Removing the Head To remove the head from the pod 1 Make sure the head is completely in the down position D axis before proceeding with removing the head This helps to ensure that the seals are protected during removal 2 Using the 9 64 Allen wrench provided remove the four plunger screws from the plunger plate Figure 2 5 in the sequence designated in Figure 2 6 CAUTION Before removing the fourth shoulder screw take hold of the A N head firmly to make sure it does not fall once all screws are removed Note Itis very important to remove the plunger screws in the correct sequence before removing the shoulder screws in the correct sequence Biomek NX Multichannel Liquid Handler User s Manual 2 8 Multichannel Pod 3 With the same 9 64 Allen wrench remove the four shoulder screws from the mandrel plate Figure 2 5 in the sequence designated in Figure 2 6 Ar ee E ES an Th Plunger plate Mandrel plate D Plunger l if screws 2 Shoulder g g screws li Figure 2 5 Plunger screws and shoulder screws removed from multichannel head Beckman Coulter Inc Multichannel Pod yA Figure 2 6 Sequence for removing four plunger screws and four shoulder screws on all multichannel heads 4 Slide the head down and out of the
17. 144 cm and teach position P9 Press OK to continue or Cancel to abort J Cancel Figure 4 6 Confirm 5 Visually verify that the framing probe is positioned to avoid hitting the wall of the AccuFrame and that it is positioned to lower within the AccuFrame tool 6 Choose OK Note The pod lowers and moves around inside the AccuFrame automatically until it breaks both light beams simultaneously Figure 2 1 The pod stops after teaching framing is completed and the two light beam indicators are illuminated Note An error message appears if both light beams are not broken when the pod is lowered into the AccuFrame If this occurs using Advanced Manual Control refer to Chapter 5 Manually Controlling the Biomek NX in Biomek Software move the pod until the probe breaks both light beams and all the indicator lights are on Choose Teach and the pod continues the teaching process The pod may also be moved by hand until the probe breaks both light beams and all the indicator lights are on Beckman Coulter Inc Framing Instructions 4 9 7 Wait until the pod stops moving and Teaching Instructions appears Figure 4 10 11 12 13 7 Teaching Instructions The location is 58 597 cm 18 751 cm 16 750 cm The change is 3 937 cm 0 088 cm 1 050 cm What would you like to do C Shift ALP Shift position Cancel Figure 4 7 Teaching Instructions
18. 2 Pod e Head Holds mandrels and tips for performing full plate replication refer to Section 2 3 Interchangeable Heads e Grippers Grip and reposition labware by gripping along the long side of the labware refer to Section 2 4 Grippers Pod Mandrels Grippers Figure 2 2 Multichannel Pod main components Biomek NX Multichannel Liquid Handler User s Manual 2 4 Multichannel Pod 2 2 Pod The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions 2 2 1 Pod Movements The Multichannel Pod performs movements in the Y Z and D axes see Table 2 1 Table 2 1 Multichannel Pod Axes Movement Y Entire pod moves front to back Z Entire pod moves up and down D Up and down aspirate dispense disposable tip shucking and close open gripper 2 2 2 Control Modes Commands entered via Biomek Software on the host computer control the operations of the Multichannel Pod Manual control associated with the operation of the Multichannel Pod is accessed through Biomek Software Note Refer to Chapter 5 Manually Controlling the Biomek NX in Biomek Software for information on manually controlling the Multichannel Pod Beckman Coulter Inc Multichannel Pod 2 5 2 3 Interchangeable Heads An interchangeable mu
19. 3 and squeeze the grippers until they firmly touch the AccuFrame inside the notches at the bottom of the AccuFrame Figure 4 24 If necessary refer to Chapter 5 Manually Controlling the Biomek NX in Biomek Software for instructions on moving the grippers Note Both front and rear grippers must touch at the same time when squeezing which usually necessitates a move along the Y axis Note Assessing the position of the grippers in step 8 is a visual task Figure 4 23 Gripper framing position on the AccuFrame Correct Gripper Alignment ncorrect Gripper Alignment Bottom of gripper finger aligned Top of gripper finger aligned to to bottom of AccuFrame and top of cut out of AccuFrame aligned to the inside of cut out Without aligning to inside of cut out Figure 4 24 Correct and incorrect gripper framing alignment 9 In Frame Gripper choose OK Figure 4 22 The grippers are now framed Biomek NX Multichannel Liquid Handler User s Manual 4 26 Framing Instructions 10 From Hardware Setup choose Accept to save changes and close Hardware Setup 11 From Biomek Software choose Instrument gt Manual Control Manual Control appears
20. 7 ShakerALPt inane fag 53 fa 55 58 434 0614 AA 7 P eigen fas 23 2 f22 229 2 404 _ Frame Gripper oF Stirrer EF StirrerALP1 Sax say _sez SA Change Head oF Tiploader0 47 Tiploader1 Additional Pod Settings 6 Digital Devices H WashPump1 Tip And Gripper Settings a e Simulator e Camera E Materials Stacker Carousels BiomekNx MC Figure 4 18 Hardware Setup Beckman Coulter Inc Framing Instructions 4 23 4 Choose Frame Gripper 5 When a Warning appears make sure the topics that appear in the Warning are addressed and choose OK Figure 2 14 1 The grippers cannot be holding anything for this operation Make sure they have been retracted aoa ee I lat between the pod and the location that you will frame the grippers at such as a stack of Ea Figure 4 19 Warning to make sure grippers are ready for framing 6 In Pick Position Figure 4 20 select a previously framed deck position and frame the grippers to that location The deck position must be framed first and should be a small passive ALP if possible Note Make sure that the AccuFrame is correctly placed on the selected position refer to Section 4 2 3 Installing AccuFrame Pick Position x Place the framing tool on a patien that has been framed select that position below and hit Ok OOo Pe Biz P18 P19 Cancel Figure 4 20 Pick previously framed deck position to frame the
21. AccuFrame framing tool Framing the Biomek NX instrument includes Framing Deck Positions on the Biomek NX Instrument Using AccuFrame Section 4 2 Manually Framing Deck Positions on the Biomek NX Instrument Section 4 3 Framing the Grippers Section 4 4 Biomek NX Multichannel Liquid Handler User s Manual 4 2 Framing Instructions 4 2 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame The AccuFrame is a tool used for framing ALP and labware positions on the Biomek NX deck Figure 4 1 Framing using the AccuFrame tool requires no human judgment of alignment and is reproducible Hard stop AccuFrame light beams Power light grippers cutout grippers are framed in the cutout at the bottom of both sides of AccuFrame light frame tool beam indicators Figure 4 1 AccuFrame framing tool The AccuFrame fits snugly on an ALP and a teaching process is performed through Biomek Software to obtain the coordinates for each deck position The framing is completed by breaking two light sensors on the AccuFrame at their intersection point The coordinates for each ALP are generated automatically through the software based upon teaching one position however when precision is critical as when using 384 well plates frame each position before using the Biomek NX instrument This ensures that the pod and grippe
22. Choose Shift deck when framing the first location of a new deck The shift amount required typically is not large but everything on the deck may need to move cm for example e Shift ALP shifts the entire ALP and all deck positions associated with the ALP by the change amounts shown Shift ALP typically is precise enough for using 96 well plates e Shift position shifts only the deck position containing the AccuFrame by the amounts shown Shift position is the most precise teaching procedure and it is useful when 384 well microplates are used especially on larger ALPs such as the 4x4 otherwise Shift ALP is usually sufficient Note When framing a multiple position ALP 1x3 1x5 4x3 or 4x4 Shift ALP on the first position then Shift position on the rest Beckman Coulter Inc Framing Instructions 4 11 4 3 Manually Framing Deck Positions on the Biomek NX Instrument Biomek NX Manual Framing Wizard is a wizard type interface that is used to manually frame deck positions primarily for using high density labware Since the wells of high density labware are relatively small using the Manual Framing Wizard helps to ensure that the tips can access the wells without causing any damage to the tips probes pod or ALP Note Some ALPs such as the Positive Position ALP must be framed using Manual Teach to improve pipetting accuracy to high density labware Note When not using high density labware the standard fr
23. E REEE RE ERR vi Chemical and Biological Safety 0406 ccv o 40 44 eae nnee vii Moving Parts ass mesas aae Gon at wlan bp eaten Seg tn stead Sk Becta les ee at viii Cleaning rerperr EEEE EN EE ees bee EE Fre ca viii Mamtenance son reri ce ky eoi seie a ea E EE EE haw A ERa eases viii Warnings and Cautions Found in this Manual 0 00000 cece ee eens ix Table of Contents ssanannnnaanan anunn xi List Of Figures soccssr r enreret en ood E ee eee E XV List f Tables ieee heat ere eer eee eee aan xvii 1 Biomek NX Multichannel Instrument Introduction 1 1 Instrument Overview ic 6 sab crror 6a oe 45S Eh 0 tearra ORES EER OS 1 1 1 1 1 Control Mod s si lo das dances ee daw aeaii darane inaia ea iaaa 1 1 1 2 System Components ssssas AREA ERED SERA EERE EAR RED ERE EDS 1 2 1 2 1 MLO WGIS eei a ocd actare arte ects a e e D E acre mee E arabe het atl as oe ators 1 3 1 2 2 Multichannel Pod 9 625900 se44 05 00459 4 eee nd bana a ede R Ewe RE eA 1 5 1 2 3 DCO EEEE EE E E E EE EE 1 6 2A ALPS erea a ete ns E A A E a 1 6 1 2 5 Device Controller 24 unuau naunau nener 1 6 1 2 6 OptionaliDevices lt cickscto ted vietetetwettotegsiedaeedatoawae 1 6 1 2 7 Protective Barriers 2 014 ca din ta aeeden dee de deka aas kd haem enka 1 7 Biomek NX Multichannel Liquid Handler User s Manual xii Table of Contents 1 3 1 4 Troubleshooting nannan unanunua 1 9 1 3 1 Changing FUSES ueue cwadietset ew Set acwad d
24. Save Settings Restore Settings Biomek NX SN None fj AccuFrame Delete Settmgs 96 Podi amp Devices 7 DeviceControllerO oF ShakerALpo 7 ShakerALP1 oF StirrerALPO 7 StirrerALP1 47 TiploaderO 7 Tiploader1 Digital Devices E Simulator e Camera E Materials Stacker Carousels Head Type 96 Mandrel 200 pL Head Last Validation Not Specified Set Validation Time Axis Limit Settings x cm Y em a a 33 2 Set Minimum Maximum we E Z cm mss pz Setz D pl D cm 58 434 osr 4 229 B 404 Set D Additional Pod Settings Tip And Gripper Settings Gripper Settings pp g X em Gripper Offset j 43764 Y cm Gripper Retract Time fa 55 s 0 92 Z cm 6 5532 Gripper Extend Time 0 55 s M Enable Plate Sensor lt D cm jos Correlate Pods Frame Gripper Change Head Enable Plate Sensor Tip Settle Time M Tip Load Settings e s Final D Position fo em Tip Unload Settings 7 Tip Unload Time Unload Speed Final D Position f30 jo cm BiomekNx MC Figure 3 4 Hardware Setup displaying Enable Plate Sensor Biomek Software Error in Move Labware from P3 to Orbitall Eror during Assert labware gripped There was no plate sensed by the orippers 2 17 2004 11 05 22 AM Figure 3 5 Error for no plate sensed Biomek
25. TION Before selecting OK to home all axes the Following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted E Cancel Figure 5 3 Warnings must be addressed before homing process begins 3 Choose OK after confirming that the actions have been addressed appropriately Biomek NX Multichannel Liquid Handler User s Manual 5 4 Manually Controlling the Biomek NX in Biomek S Beckman Coulter Inc 5 3 2 Moving the Pod to a Specific Deck Position Use Manual Control to easily move the pod to a specific deck position Manual Control moves the pod to the top of the Z axis then centers it over the selected position To move a pod to a specific deck position 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Click on the desired deck position on the Manual Control Deck Display Figure 5 2 5 3 3 Stopping the Pod To stop a pod once a movement has started 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Choose Stop 5 3 4 Viewing the Firmware Version Get Version shows the current firmware version for installed devices pods and main firmware To view the firmware version 1 Choose Instrument gt Manual Control Manual Control appears Figur
26. UTION To avoid hardware crashes a new D axis limit must be established and the grippers framed in the Biomek Software after the head has been configured in Hardware Setup To change the head on a Multichannel Pod refer to Section 2 3 1 Changing Heads and then configure Hardware Setup according to the following instructions 1 In Hardware Setup select the Pod 2 Change the Serial Number to correspond to the serial number on the new head Biomek NX Multichannel Liquid Handler User s Manual 3 6 Configuring the Biomek NX MC in Biomek Softy 3 Choose the appropriate head in Head Type Figure 3 2 Reconnect i Home All Axes cp Add Device m Remove Device J Accept gt lt Cancel Bi NX SN N 7 A gt Fa one Serial Number None Save Settings Restore Settings Delete Settings 96 Head Type 96 Mandrel200pL Head Last Validation Not Specified Set Validation Time FER Devices i Aris Limit Setting g DeviceController0 x em F ShakerALPO Y cm Z cm D ul D cm eP e ShakerALP1 Minimum ss 53 jiss 58 434 fos Frame Gripper A MEAT Maximum fas 33 2 f22 229 2 404 Tiploader0 Bex sety Set Z Set D Change Head 47 Tiploader1 amp Digital Devices Additional Pod Settings El p Simulator E Camera Tip And Gripper Settings Materials Stacker Carousels BiomekNX MC Figure 3 2 Hardware
27. al number on the Biomek NX instrument 3 Choose the appropriate Port 4 Choose Accept Hardware Setup closes Note Accept must be chosen after the instrument has been configured to allow Hardware Setup to accept the configurations However other devices may be configured simultaneously as the Biomek NX instrument is configured and Accept may be chosen after all devices have been configured Note An asterisk next to the device indicates the device has been modified since the instrument was loaded Beckman Coulter Inc figuring the Biomek NX MC in Biomek Software 3 5 3 3 Configuring a Multichannel Pod gt gt The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions refer to Chapter 2 Multichannel Pod Configuring a Multichannel Pod in Hardware Setup includes e Configuring a New Head Section 3 3 1 e Setting Multichannel Pod Properties Section 3 3 2 as instructed by a Beckman Coulter Service Engineer Note The settings for a Multichannel Pod can be saved restored and deleted refer to Section 3 4 Saving Restoring and Deleting Settings 3 3 1 Configuring a New Head When a head on a Multichannel Pod is changed the Hardware Setup must be changed appropriately CAUTION If the hardware configuration is not updated using Hardware Setup hardware crashes or inaccurate liquid transfer may occur CA
28. aming procedure using the AccuFrame is acceptable To frame using the AccuFrame refer to Section 4 2 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame To frame using Manual Teach 1 Choose Start gt Programs gt SAGIAN Software gt Biomek Software to open Biomek Software 2 From the Instrument menu choose Deck Editor Deck Editor appears Figure 4 8 Deck1 Default Deck E x t a New Deck Delete Deck Rename Deck OpenDeck Clear Deck Renumber Delete ALP Properties Save Cancel PositivePositionALP ShakerALP Span8ActiveWashLefl SpansActiveWashRigl Span TipTrash Span8TrashLeft xl 7 Coords cm fo jo fo p lt Figure 4 8 Deck Editor Biomek NX Multichannel Liquid Handler User s Manual 4 12 Framing Instructions 3 Open Position Properties for the desired deck position by double clicking on the deck position Position Properties appears Figure 4 9 Position Properties Name Pi ALP Type FourByThreeHD x cm cm zZ cm Precision Podi Coordinates 6 645 jis 436 16 4 Not Framed Advanced MC Teach More gt gt Manual Teach Auto Teach emes Figure 4 9 Position Properties for a Positive Position ALP 4 In Name verify that the ALP is assigned a unique name Beckman Coulter Inc 5 Choose Manual Teach Biomek NX Manual Framing Wizard opens with a Warning Figure 4 10 Note On the left side
29. ar Speed 100 Go Stop a Builds a vector to an absolute coordinate from the current coordinate Immediate Moves Executes the specified action immediately Home D Home Z XY Extend Gripper Retract Gripper and Move Z Max are immediate moves D Units Sets the D axis units Choose cm to set squeeze units and or uL to set aspirate units Current Position Displays the current position of the selected pod Movement Vector Indicates the amount the pod moves when Go is selected Figure 5 5 Overview Advanced Manual Control for a Multichannel Pod See Table 5 1 for further descriptions of fields Beckman Coulter Inc Controlling the Biomek NX in Biomek Software 5 7 Table 5 1 Advanced Manual Control selection areas for the Multichannel Pod Refer to specific subsections for instructional use Area Absolute Move Description Builds a vector to an absolute coordinate from the current coordinate Auto Clear When checked each time Go is selected the Movement Vector resets to the 0 vector no movement Note Auto Clear is on by default Clear Sets movement vector entries to 0 Current Position Current location of the pod after the pod has been homed D Units Sets the D axis units Note Choose cm to set squeeze units or uL to set aspirate units Delta Sets the magnitude of chan
30. arge circle Note When the move is completed the small circle resets itself to the center of the large circle The values displayed in Total Moved from Start cm changes each time steps 1 and 2 are completed If desired the values in Total Moved from Start cm can be reset to zero by selecting Reset 3 Visually verify the position of the tips on the pod in relation to the physical position of the wells of the microplate on top of the ALP If the tips are still not accurately positioned above the target position on the microplate repeat steps 1 and 2 until they are accurately positioned above the target position the microplate Note The tips may also be aligned with the microplate using the delta value and directional buttons refer to Section 4 3 2 Using the Delta Value and Directional Buttons 4 Once the tip is aligned with the wells of the microplate on top of the ALP choose Next to continue Biomek NX Manual Framing Wizard displays Teach Z refer to Section 4 3 3 Framing the Z Axis Biomek NX Multichannel Liquid Handler User s Manual 4 18 Framing Instructions 4 3 2 Using the Delta Value and Directional Buttons The tip can be positioned manually using the delta values and directional buttons to align it with the physical position of the wells of the microplate on top of the ALP Pressing one of the directional buttons moves the pod in that direction by the distance specified in Delta Position the tip
31. arrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such one year period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows a Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list of such components and accessories is maintained at the factory b The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company will give reasonable assistance to Purchaser in obtaining from the manufacturer s own warranty c Any product claimed to be defective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportatio
32. ble tips loaded e Make sure the Framing Probe is NOT installed on the Multichannel Pod e Make sure the grippers on the Multichannel Pod are retracted WARNING Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck WARNING Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately WARNING Make sure the AccuFrame cable does not interfere with pod movement CAUTION Use Shift deck only before other ALPs or positions are taught Shift deck shifts all ALPs and positions resulting in incorrect coordinates if applied to previously framed ALPs and positions CAUTION Select Move Z Max to move the pod to its highest point before extending the grippers To avoid breaking labware or bending the grippers make sure the grippers will not hit any labware when extended CAUTION Do not retract grippers when they are holding labware Table of Contents Warranty and Returned Goods Requirements 00 cece cece eee iii Safety Information 1 ccac oceans Cee ee eee eee annann v Warning and Caution Deniniiions 2 5 242 lt 944e5 bnese bee buen dons bie ead cesentaeks v PT Salete sera ee ar E E EE TEE E EE en E vi High VONAGE 6 en eh hee ed athaneeg Rhee Ped EEA GEE EE erena vi L ser Light cated heteutetwertovatheser tet aa E e a E aa aa enaa is vi Laser Specifications 46 on ican sea Cid eh AGES AE EE ER
33. bserve the cleaning procedures outlined in this user s manual for the Biomek NX liquid handler Prior to cleaning equipment that has been exposed to hazardous material e Appropriate Chemical and Biological Safety personnel should be contacted e The Chemical and Biological Safety information contained in this user s manual should be reviewed Perform only the maintenance described in this manual Maintenance other than that specified in this manual should be performed only by Beckman Coulter service representatives It is your responsibility to decontaminate components of the Biomek NX liquid handler before requesting service by a Beckman Coulter service representative or returning parts to Beckman Coulter for repair Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so If any parts are returned they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated Safety Information ix Warnings and Cautions Found in this Manual Please read and observe all cautions and instructions Remember the most important key to safety is to operate the Biomek NX liquid handler with care The WARNINGs and CAUTIONSs found within this document are listed below WARNING Dark non reflective material affects the sensitivity of the light AN curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex glo
34. ce coe suue caataesennsus sus tesansniceacesturheseanatsetrcumtacsneuatusuetme cabins 2 12 Figure 2 10 Removable front gripper assembly cccceccceesseceseceeceesceceaeceseeeeeeesaeecsseeneenees 2 17 Figure 3 1 Hardware Setup for a Biomek NX Multichannel instrument 0 cc eeeeeeeeeeeeeeeee 3 2 Figure 3 2 Hardware Setup showing the configuration of a Multichannel Pod eee 3 6 Figure 3 3 Warnings must be addressed before homing process begins ccccesceeeeeeeteeeeees 3 6 Figure 3 4 Hardware Setup displaying Enable Plate Sensor c ccceccceeseeeseeeseeeeeeeteeeeeeeeaees 3 9 Figur 3 57 Error for 10 plate Gensel az ccisssateianccayisniatcavagaaaesteeaneeeanianieatat deca 3 9 Fig re 3 6 Save ONT BUCA OM seses vseaiuaddssapncavsdanveescseniteesadincnsaaentnenesoniansiesnansauenplavdeannacisins 3 10 Fig r 3 7 Load S ttingS incer irei E EAEE A A 3 11 Figure 3 8 Confirmation to reset stored configuration sssesesssesssseessessresresseeseesresseesresressee 3 11 Figure 3 9 Delete SEU ies lt sacezcecaces hvecausnaneatceesetecccancestvvastceeveeninceanesmeaaeurasneicorecacurentiaeueetnnes 3 12 Figure 3 10 Confirmation to delete stored configuration ccccecsecssecsseceeeceeeeeeseceeenteeeees 3 12 Figure 4 1 AccuFrame framing tool cvicsectasnecinsvensvacasenenncaendetenssiends seavexeinnansnuensatieeenninencinentonnies 4 2 Figure 4 2 Warning to address before homing process begins
35. ceable grippers Beckman Coulter Inc gt gt Multichannel Pod 2 17 2 6 1 1 Removing Gripper Fingers WARNING Avoid all labware on the Biomek NX deck when removing or installing grippers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware CAUTION Use caution when removing grippers Since grippers mount tightly into the Multichannel Pod they pull free of the Multichannel Pod suddenly To remove grippers 1 Move the Multichannel Pod to the maximum Y and Z axis limits refer to Section 5 4 Using Advanced Manual Control 2 Grasp both sides of the gripper Figure 2 10 3 Apply force in a downward direction to pull the gripper from the head Front gripper assembly Gripper shaft ra Gripper pads Front of gripper Figure 2 10 Removable front gripper assembly Biomek NX Multichannel Liquid Handler User s Manual 2 18 Multichannel Pod 2 6 1 2 Installing Gripper Fingers WARNING Avoid all labware on the Biomek NX deck when removing or AN installing grippers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware To install gripper fingers 1 Beckman Coulter Inc Move the Multichannel Pod to the maximum Y and Z axis limits refer to Section 5 4 Using Advanced Manual Control
36. channel Pod Biomek NX Multichannel Liquid Handler User s Manual 2 12 Multichannel Pod 2 4 Grippers The Multichannel Pod incorporates a gripper tool grippers Figure 2 9 that grasps and moves labware from one location on the Biomek NX deck to another Grippers can e move labware no taller than 2 35 inches in height move stacks of standard height labware no more than four plates high maximum 2 35 inches Note Grippers stack and unstack groups of labware from the bottom Ifa stack containing four pieces of labware is being unstacked the grippers grasp the top three and move them to a new position Then the grippers grasp the top two of that stack and move them finally the grippers grasp the top piece of labware in that stack and move it to a new location e place lids on and remove lids from labware e remove lids from tip boxes Note Grippers do not place lids on tip boxes Gripper shaft Gripper shaft Gripper fingers Figure 2 9 Grippers The grippers contain two fingers e a double gripper located to the front of the instrument e a single gripper located to the back of the instrument Beckman Coulter Inc Multichannel Pod 2 13 2 4 1 Framing Grippers WARNING Grippers may bend if not taught framed properly with the UN AccuFrame Grippers are framed during installation by a Beckman Coulter Service Engineer The
37. ckman Coulter Service Engineer Grippers are not deploying Check input air pressure Contact a Beckman Coulter Service Engineer Power is lost to the Y axis Contact a Beckman Coulter Service Engineer The head does not work properly Contact a Beckman Coulter Service Engineer Aspiration or dispense actions are not accurate Contact a Beckman Coulter Service Engineer A mandrel on the head is damaged Contact a Beckman Coulter Service Engineer the head may need to be returned to the factory for repair or replacement Front gripper shaft is bent Front gripper pads look worn Replace the front gripper fingers using Front Removeable Rod Assembly Beckman Coulter Part Number 394062 Refer to Section 2 6 1 Replacing Grippers for instructions Rear gripper shaft is bent Rear gripper pads look worn Replace the rear gripper fingers using Rear Removeable Rod Assembly Beckman Coulter Part Number 394063 Refer to Section 2 6 1 Replacing Grippers for instructions Biomek NX Multichannel Liquid Handler User s Manual 2 16 Multichannel Pod 2 6 1 Replacing Grippers Grippers are easily replaced by pulling the damaged grippers out of the head and pushing the replacement grippers into the head Pod front Front removeable Rear removeable gripper assembly gripper assembly Figure 2 1 Installed repla
38. d strap when removing a multichannel head Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek NX instrument Figure 2 3 Strap portion of wrist 4 ground strap snugly attached to the wrist Alligator clip clamped to the instrument deck base plate Figure 2 3 Wrist ground strap attached to the deck The same method is used to remove the 96 Channel 20 uL Head 96 Channel 200 uL Head and 384 Channel 30 uL Head Removing a head from the Multichannel Pod includes removing the bottom cover e removing the head 2 3 1 1 1 Removing the Bottom Cover To remove the bottom cover to expose the head 1 In Hardware Setup select the appropriate Multichannel Pod Note A Multichannel Pod is identified with a 96 or 384 2 Choose Change Head Note Choosing Change Head moves the D axis to 0 6 cm and extends the grippers 3 Turn off power to the Biomek NX instrument 4 Position the strap portion of the wrist ground strap snugly around the wrist 5 Clamp the alligator clip of the wrist ground strap to the instrument deck base plate Figure 2 3 Beckman Coulter Inc Multichannel Pod 2 7 6 Using a Phillips screwdriver remove the three screws from the plastic bottom cover Figure 2 4 Figure 2 4 Bottom cover removed from the pod 7 Carefully open and remove the bottom cover Note Do not remove
39. e 5 2 2 Choose Get Version The firmware version displays in an Information dialog similar to Figure 5 4 r atio tJ Nx Master Controller 3 05 J2 Left arm Y Board 21 0 1 7 BL 0 0 10 Left arm Y Span Board alge oF 7 BL 0 0 10 Left arm 21 4 Board 23 0 1 7 BL 0 0 10 Left arm 25 8 Board 24 0 1 7 BL 0 0 10 Left arm Pump Board 26 0 4 5BL 1 6 0 Left arm Gripper Board 28 0 1 7 BL 0 0 10 Framing Tool 5 0 2 2 1 BL 0 1 3 62 00 62 0 1 2 26 BL 2 0 0 68 00 68 0 1 1 5 BL 2 0 0 Figure 5 4 Firmware version information 3 To close Information choose OK 5 3 5 Accessing Advanced Manual Control To access Advanced Manual Control 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Choose Advanced Controls 3 Select a device from the menu Advanced Manual Control for the selected device appears Controlling the Biomek NX in Biomek Software 5 5 5 4 Using Advanced Manual Control Use Advanced Manual Control to e View the current position of the pod e Move the pod to a safe roving height e Build and apply movement vectors to the pod e Allow small movements that are useful when teaching the deck or framing the grippers e Extend and retract the grippers 5 4 1 Viewing the Current Position the Pod The current position of the pod is displayed as four coordinates Each axis coordinate is the distance from the home position X Y and Z are displayed
40. e Restrictions up to 2000m 6562ft Installation Category Category II Pollution Degree 2 Fuses US 250VAC 3 15 amp 5x20 mm SLO BLO UL recognized CSA certified Europe 250VAC 3 15 amp 5x20 mm SLO BLO CENELEC approved Communications to Host RS 232 port Biomek NX Multichannel Liquid Handler User s Manual Table A 1 System Specifications Continued Item Description Communications to Tip CAN Loader Communications to CAN Active ALPs Biomek controller host The following specifications are the minimum PC requirements needed for the IBM PC CPU Pentium IV 2 GHz RAM 512 MB Hard Drive 13 5 GB CD ROM Drive 24X Monitor 17 Super VGA 1024 x 768 small fonts w 16 bit color Operating System Windows NT 4 0 with Service Pack 6a Windows 2000 with Service Pack 4 or Windows XP with Service Pack la Other Software SQL Server Personal Edition Beckman Coulter Inc Index Numerics 384 Channel 30uL Head description 2 5 96 Channel 200uL Head description 2 5 A Absolute Move using in Advanced Manual Control 5 12 AccuFrame calibrated by Beckman Coulter Service Engineer 4 2 defined 4 2 installing the 4 6 troubleshooting 4 27 using the 4 6 Advanced Manual Control accessing 5 4 defined 5 1 selection areas for Multichannel Pod 5 7 ALPs overview 1 6 B Biomek NX described 1 1 main components ALPs 1 6 deck 1 6 Device Controlle
41. e tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target Delta pey em A Back Left Bight A s Ewd figs IV Hysteresis compensation Total Moved From Start cm C Well Comer Well Center Finish Graphic alignment tool The graphic alignment tool is a visual representation of the tip small circle and the microplate wells or corner The small circle is moved until it represents the tip s current physical location in relation to the microplate wells or corner cont tor Figure 4 15 Manual Teaching the X and Y axes Beckman Coulter Inc Framing Instructions 4 17 To use the graphic alignment tool 1 Drag the center small circle until it represents the tip s physical position in relation to the physical position of the wells of the microplate on top of the ALP Note The small circle represents the tip on the pod The objective is to provide the software with a representation of the tip s position in relation to the physical position of the wells of the microplate on top of the ALP The software uses this graphical representation to know approximately how far in any direction the pod must move to align the tips with the wells of the microplate on top of the ALP 2 Select Go The pod moves in accordance with the position of the small circle in relation to the l
42. ed to the Biomek NX Multichannel instrument to accommodate specific operations Refer to Appendix B Using a Stacker Carousel lomek NX Multichannel Instrument Introduction 1 7 1 2 7 Protective Barriers The Biomek NX Multichannel instrument has safety measures to protect the Biomek NX operator Safety features of the Biomek NX liquid handler include a front light curtain and side safety shields Figure 1 5 Note An optional side safety shield allowing device integrations is available Light curtain Side safety shield Figure 1 5 Protective barriers for the Biomek NX Instrument Biomek NX Multichannel Liquid Handler User s Manual 1 8 Biomek NX Multichannel Instrument Introd 1 2 7 1 Light Curtain Protection System WARNING Dark non reflective material affects the sensitivity of the light AN curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex gloves do not degrade light curtain operation however it is advisable to test the impact of the all lab dress on light curtain sensitivity before operating the Biomek NX Verify lab dress impact on light curtain sensitivity as follows Use Manual Control in Biomek Software and insert the material no more than 1 past and approximately 21 above the light curtain panel Make sure the solid amber indicator light changes to blinking amber The diffuse reflective perimeter light curtai
43. elete Deck Rename Deck Open Deck Clear Deck Renumber Delete ALP Properties Save Cancel Coords cm o 903 13 436 fis 935 f Figure 4 4 Deck Editor 2 In Biomek Software open the deck that requires framing in the Deck Editor Verify that it reflects the current configuration of ALPs on the physical deck If it does not reflect the current physical deck configuration place devices on the appropriate deck locations in the Deck Editor by dragging and dropping from the list onto the deck When the Deck Editor reflects the configuration of the current physical deck proceed to step 3 Biomek NX Multichannel Liquid Handler User s Manual 4 8 Framing Instructions 3 Double click on the deck position containing the AccuFrame Position Properties appears Figure 4 5 Note The coordinates displayed are default values the software must be taught precisely where the position is on the physical deck Position Properties Name Py ALP Type FourByThreeHD x cm cm Z cm Precision Podi Coordinates 6 645 13 436 16 4 Not Framed Advanced MC Teach More gt gt Manual Teach Auto Teach cancel Figure 4 5 Position Properties 4 Choose Auto Teach A Confirm similar to Figure 4 6 appears Note This moves the pod to the position being taught The framing probe should be above the AccuFrame in that position Q The pod is about to go down 5
44. ense Delta D Sets the distance the head or grippers move in the D axis Subtracts Delta D from movement value Note Positive values move the pod to the right X toward the front of the deck Y up Z and squeeze aspirate D Negative values move the pod to the left X towards the back of the deck Y down Z and unsqueeze dispense D Note Minimum and Maximum X Y Z and D values displayed in Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors Enter a value in Speed to specify the percent of the pod s maximum speed Choose Go The pod moves from its current position to a new position by the values displayed in the Movement Vector The new position is displayed in Current Position Choose Close to close Advanced Manual Control Choose Exit to close Manual Control Biomek NX Multichannel Liquid Handler User s Manual 5 12 Manually Controlling the Biomek NX in Biomek 5 4 6 Performing Absolute Moves for the Pod Absolute Move allows the pod to move to a specific coordinate position in the workspace of the instrument Use Absolute Move when the coordinates of the desired position are known Note When an Absolute Move is entered the values displayed in Movement Vector reflect the relative move required to physically move the pod to the desired position Make sure the physical locatio
45. ent power switch 2 Unplug the instrument from the power source 3iomek NX Multichannel Instrument Introduction 1 11 3 Using a small flathead screwdriver pry open the fuse carrier containing two fuses Figure 1 6 from the AC power input module Figure 1 6 Replacing fuses in tower 4 Gently pull old fuses from the carrier by hand 5 Gently place new fuses into carrier by hand Note Use 250v 3 15 amp 5x20 Slow Blow type fuses for replacement 6 Replace the fuse carrier into the AC power input module 7 Plug the instrument into the power source 8 Turn on the instrument power switch Biomek NX Multichannel Liquid Handler User s Manual 1 12 Biomek NX Multichannel Instrument Introc 1 4 Preventive Maintenance To ensure optimum operation of the Biomek NX liquid handler perform the following maintenance procedures as necessary WARNING To prevent injury use proper decontamination procedures e Wipe up all spills on the deck immediately e Approximately every 100 km of travel or every six to twelve months schedule a Beckman Coulter service call to lubricate the linear rail e Once a week verify proper operation of light curtain using Manual Control in the software refer to Chapter 5 Manually Controlling the Biomek NX in Biomek Software and the light curtain test rod supplied with Biomek NX Insert the test rod no more than 1 past and approximately 21 above the front light curtain
46. ere D 58 43 229 00 Speed Limit 100 Additional Roving Height 0 75 cm Additional Timeout 25 00 s Do not move to Z max when roving Gripper Settings Gripper Offset xl Cmar Figure 3 9 Delete Settings 3 Select the desired settings to delete 4 Choose OK The following Confirm appears Figure 3 10 Confirm Q Are you certain you want to delete the stored configuration MultichannelPodSettings1 Click Yes to delete MultichannelPodSettings1 Click No to return to Hardware Setup without deleting Figure 3 10 Confirmation to delete stored configuration 5 Choose Yes The stored configuration is deleted Beckman Coulter Inc 4 1 Framing Instructions Overview Framing is the procedure of teaching Biomek Software the location of the ALPs and devices positioned on the deck Biomek Software uses this framing information to move the pod or grippers to the appropriate position to perform liquid handling operations and manipulate labware A Beckman Coulter Service Engineer frames the Biomek NX instrument during system installation It may be necessary to repeat the framing if e ALPs or devices are added moved or removed from the deck e the head on the Multichannel Pod is changed Framing deck positions can be performed automatically using the AccuFrame framing tool or manually using a piece of labware to visually align the pod to the wells The grippers may also be framed using the
47. es the D axis to 0 6 cm and extends the grippers to remove and install a head 3 3 2 Most of the properties including pod settings and axes limits of a Multichannel Pod are initially configured by a Beckman Coulter Service Engineer in Hardware Setup and should not be modified without specific instructions from a Beckman Coulter Service Engineer or Beckman Coulter Technical Support Setting Multichannel Pod Properties CAUTION Do not make any changes to the pod axes limits without contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support Table 23 2 describes the pod properties and axes limits listed in Hardware Setup for a Multichannel Pod Table 3 2 Multichannel Pod Properties Property Additional Roving Height Description Margin above the default height for the pod as it moves over everything on the deck Additional Timeout Specifies a number of seconds to wait in addition to the normal time it takes to carry out a step before a timeout error occurs Use when experiencing timeout problems without actual hardware problems Always move to max Z when roving Check this field to move the pod to its maximum height during any move in the X or Y axis Enable Plate Sensor Enables the plate sensor on the grippers to sense labware Default setting is checked enabled Refer to Section 3 3 2 1 Using the Plate Sensor Final D Position under Tip Load Settings Positio
48. esenting the motion required to physically move the pod down until the tip just touches the microplate at the well corner or the bottom of the well Note Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction Note The tip can be can physically positioned over the labware positioner using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 3 Once the tip is just touching the target position select Finish The pod moves up to its maximum height in the Z axis Manual Teaching closes and Position Properties appears Figure 4 9 4 Choose OK to save the framing information and close Position Properties 5 Repeat the procedure to frame additional deck positions using manual teach 6 Choose Save to save framing information for all positions and close the Deck Editor Figure 4 8 Note Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened Biomek NX Multichannel Liquid Handler User s Manual 4 22 Framing Instructions 4 4 Framing the Grippers
49. etectesdaedcuvuanebineecaraenayeeeas 4 15 Figure 4 15 Manual Teaching the X and Y axes ssccssssscrsssssssessscssnessssonteassonssesevecesacsonns 4 16 Figure 4 16 Manual Teaching the X and Y axes 0 ce ceseeseeseeeeeeeeceseeeeceseceaecaeeeaeeeeeeeeeeaeenaes 4 18 Figure 4 17 Manual Teaching Teach 7 csscvissarasusstsnsapewssnedusecmnaasiactoncaieeesanceustonsaseensweiuctisdeens 4 20 Figure 4 18 Hardware Setup sccesavsatecissicpnsunscacveevenatosndebesancecedevswincasurennsauseameaceucearrarsnausracorssaiees 4 22 Figure 4 19 Warning to make sure grippers are ready for framing ccccsceesseeeteeeteeeees 4 23 Figure 4 20 Pick previously framed deck position to frame the gripperS cccceeteeeeeeeee 4 23 Figure 4 21 Advanced Manual Control for moving grippers during framing 00 4 24 Figure 4 22 Moving pod into gripping position ssccsscsseccsescssscssscsssccsescssccssacssenseneneees 4 24 Figure 4 23 Gripper framing position on the AccuFrame ceceeceeeeceseceeeeseeneeeaeeeeeeeeeeseenaes 4 25 Figure 4 24 Correct and incorrect gripper framing alignment 0 cccecceeceeeseeeseeeteeeeeeeees 4 25 Figure 5 1 Confirms Manual Control is connecting 0 0 0 cceeeseeceeseceeceeeceseceeeeseeeeeeeeeeaeceaeeneeees 5 2 Fig re 3 2 Manual Cono lecun A Ss 5 2 Figure 5 3 Warnings must be addressed before homing process begins cescceeeeeereeeeees 5 3 Figure 5 4 Firmwa
50. framing process for the grippers must be repeated when e A head is changed e Extraordinary circumstances occur such as accidentally bending a gripper Should grippers need to be framed refer to Section 4 4 Framing the Grippers Biomek NX Multichannel Liquid Handler User s Manual 2 14 Multichannel Pod 2 5 Preventive Maintenance The Multichannel Pod requires little preventive maintenance however to ensure optimum operation perform the following maintenance procedures as necessary WARNING To prevent injury use proper decontamination procedures e Wipe up all spills immediately e Return heads to their original packaging when they are not in use e Check connections periodically to make sure that all are secure refer to Section 2 6 Troubleshooting e Check and tighten head mount screws and gripper mount screws e Make sure that gripper shafts are straight and gripper pads are in good condition Replace as needed refer to Section 2 6 1 Replacing Grippers Beckman Coulter Inc 2 6 Multichannel Pod 2 15 Troubleshooting A WARNING Do not connect or disconnect any cable while power is applied to the Biomek NX Perform the troubleshooting techniques provided in Table 2 3 when necessary Note In the case of any other pod related problems contact a Beckman Coulter Service Engineer Table 2 3 Multichannel Pod Troubleshooting If Power is lost to the pod Then Contact a Be
51. ge the Vector Builder applies to the Movement Vector for each axis Extend Gripper Extends the grippers Home D Moves D axis to home position Home Z XY Moves Z and then X and Y axes to home position Move Z Max Moves the pod to highest configured height Movement Vector The amount of movement that occurs when Go is selected Movement vectors are relative to the current position Retract Gripper Retracts the grippers Speed Sets the speed of the pod with 0 percent meaning use current speed Vector Builder Relative moves which allow the pod to move from its current location to anywhere on the deck are created using the Vector Builder Each time a Vector Builder button is pressed the Movement Vector is changed in the corresponding axis by the amount indicated in Delta Up and Down move the pod in the Z axis Left and Right move the pod in the X axis and Back and Fwd move the pod in the Y axis Squeeze Aspirate and UnSqueeze Dispense move the head in the D axis based upon the selection made in D Units Biomek NX Multichannel Liquid Handler User s Manual 5 8 Manually Controlling the Biomek NX in Biomek Beckman Coulter Inc 5 4 2 Moving the Pod to a Safe Roving Height Use Move Z Max in Advanced Manual Control to move the pod to its highest configured height This helps to avoid collisions when moving the pod around the deck manually Note The possibility of
52. he deck also contains predrilled locating holes used to precisely position ALPs refer to Section 1 2 4 ALPs Note Some ALPs and devices require a Device Controller refer to Section 1 2 5 Device Controller to provide power and communications between the device and Biomek NX instrument 1 2 4 ALPs Automated Labware Positioners ALPs are interchangeable structures that are installed on the Biomek NX deck in suitable positions to accommodate a wide variety of labware ALPs are either active or passive e Passive ALPs hold labware in place or act as receptacles for method by products or consumables e Active ALPS contain mechanisms that may use power and or air sources for various mechanical operations Note Refer to the ALPs User s Manual for more information on ALPs 1 2 5 Device Controller A Device Controller is a peripheral box attached to the Biomek NX that provides a means to control a number of high voltage 1 10OVAC 220VAC digital devices low voltage 24VDC digital devices and low voltage digital inputs High voltage devices receive power through an AC Power Input while low voltage devices receive power through one of the two Controller Area Network CAN interfaces located on the back of the Device Controller Note Refer to the ALPs User s Manual Appendix A Device Controller for more information on the Device Controller 1 2 6 Optional Devices Optional devices such as a stacker carousel can be add
53. he labware placed in the position to frame Biomek NX Multichannel Liquid Handler User s Manual 4 14 Framing Instructions 9 Choose Next to continue to the next screen Biomek NX Manual Framing Wizard Teach X Y appears Beckman Coulter Inc Framing Instructions 4 15 10 Select Well Corner to align the tips to the corners or junction of four wells Note Well Corner is available only when framing to a deck position occupied by a piece of labware with square wells W Biomek NX Manual Framing Wizard 10 x The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Comer and press Go Press Next gt when the tips are directly above their target Delta pa om Setup A Back Left Right Teach X Y mae 4 Down JV Hysteresis compensation Total Moved From Start cm TeachZ o p foes Well Comer Well Center Go Finish cont tor Figure 4 13 Framing to the corner of four wells OR Select Well Center to align the tips to the center of the wells W Biomek NX Manual Framing Wizard 5 x The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target 1 Delta 0 05 H cem mn Back Left Bight Teach X Y f eac e Fue Down IV Hysteresis compe
54. hoose Clear to change the Movement Vector values to 0 5 Enter the desired X Y Z and D values in Movement Vector OR Click the appropriate buttons see Table 5 2 in Vector Builder Figure 5 6 until the desired values appear in Movement Vector Figure 5 5 Note Hold the button down to quickly add Delta value to the Movement Vector rs asc Left p Bor 4 Fwd Deve aie Squeeze Aspirate Vector Builder J UnSqueeze Dispense Figure 5 6 Vector Builder Beckman Coulter Inc Controlling the Biomek NX in Biomek Software 5 11 Table 5 2 Vector Builder Buttons for the Multichannel Pod Button Description Right Delta X Sets the distance the pod moves in the X axis Adds Delta X to movement value Left Delta X Sets the distance the pod moves in the X axis Subtracts Delta X from movement value Fwd Delta Y Sets the distance the pod moves in the Y axis Adds Delta Y to movement value Back Delta Y Sets the distance the pod moves in the Y axis Subtracts Delta Y from movement value Up Delta Z Sets the distance a pod moves in the Z axis Adds Delta Z to movement value Down Delta Z Sets the distance the pod moves in the Z axis Subtracts Delta Z from movement value Squeeze Aspirate Delta D Sets the distance the head or grippers move in the D axis Adds Delta D to movement value UnSqueeze Disp
55. hoose Start gt Programs gt SAGIAN Software gt Biomek Software to open Biomek Software 2 Choose Instrument gt Home All Axes Warning appears Figure 4 2 Note Additional Warnings and Information may appear depending on the instrument configuration Note Choosing Home All Axes homes all of the axes for all pods lt lt xl CAUTION Before selecting OK to home all axes the following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Figure 4 2 Warning to address before homing process begins 3 Choose OK on each Warning and Information after confirming that the actions have been addressed appropriately Beckman Coulter Inc Framing Instructions 4 5 4 2 2 Attaching the Framing Fixture to the Pod After homing the pod the framing fixture must be installed on the pod used for framing When positioning the framing fixture the framing probe must be pointed down and away from the mandrels of the head From the front perspective the framing guides on the framing fixture must be to the back and left To install the framing fixture on the multichannel head 1 Hold the framing fixture Figure 4 3 against the head with the framing guides pressed to the outside of the back row and left column of ma
56. icing of equipment requiring the removal of covers or panels High Voltage This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation maintenance and servicing of all modules Do not remove system covers To avoid electrical shock use supplied power cords only and connect to properly grounded three holed wall outlets Do not use multiplug power strips This symbol indicates that a potential hazard to personal safety exists from a laser source When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol Laser Specifications Laser Type Class II Laser Diode Maximum Output 11 mW Wavelength 670 Safety Information vii Chemical and Biological Safety Normal operation of the Biomek NX liquid handler may involve the use of materials that are toxic flammable or otherwise biologically harmful When using such materials observe the following precautions e Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease e Observe all cautionary information printed on the original solutions containers prior to their use e Dispose of all waste solutions according to your facility s waste disposal procedures e Operate the Biomek NX liquid handler in accordance
57. in centimeters The D axis is displayed in either centimeters cm or microliters uL Refer to the description for D Units in Table 5 1 To view the current position of a pod 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 5 5 The current position is shown in Current Position 4 Choose Close to close Advanced Manual Control 5 Choose Exit to close Manual Control Biomek NX Multichannel Liquid Handler User s Manual 5 6 Manually Controlling the Biomek NX in Bio Controls the amount of change applied to the Vector Builder Delta Builds a movement vector controls the direction and distance the pod moves These choices change Movement Vector when the Vector the values in the Movement Vector displayed Builder is used below Home D Advanced Manual Control Pod1 Multichannel Moves D axis to Vector Builder the home Up me position Home Z XY t uae Home Z XY Extend Gripper Left 4 ee aa eds Moves Z and then X and Y Retract Gripper Fwd E axes to home Down positions Move Z Max gt lt e gt Move Z Max peace Squeeze Aspirate UnSqueeze Dispense Moves pod to M Current Position e x 0324 cm y 0102 om z f3 om D 0 336 configured height M Movement Vector x fo om Y 0 cm Z 0 cm D fo Absolute Move M AutoClear 2 Cle
58. ings Information MultichannelPods ettings Validation Time Not Validated Serial Number Head Type 96 Mandrel 200 pL Head Desired settings to load are selected here Z 15 50 2 20 D 58 43 229 00 Speed Limit 100 Additional Roving Height 0 75 cm Additional Timeout 25 00 s Do not move to Z max when roving Gripper Settings Gripper Offset Ceai Figure 3 7 Load Settings 3 Select the desired settings 4 Choose OK The following Confirm appears Figure 3 8 C N Are you certain you want to reset your pod configuration to the values in the stored configuration 2 MultichannelPodSettings1 Click Yes to modify the current pod configuration Click No to retain the current pod configuration Figure 3 8 Confirmation to reset stored configuration 5 Choose Yes The stored configuration is reset Biomek NX Multichannel Liquid Handler User s Manual 3 12 Configuring the Biomek NX MC in Biome G 3 4 3 Deleting Pod Settings To delete settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane 2 Choose Delete Settings Delete Settings appear Figure 3 9 Delete Settings Settings Settings Information MultichannelPodS ettings Validation Time Not Validated Serial Number Head Type 96 Mandrel 200 pL Head Select desired settings Z 15 56 2 20 to delete h
59. ions in Hardware Setup Table 3 1 Hardware Setup Options Option Description Reconnect Allows Hardware Setup to reexamine the devices present Choose this option to determine what devices are present rather than closing and reopening Hardware Setup Home All Axes Provides the Biomek NX Multichannel instrument with a point of reference from which to make subsequent moves Home position is left back Note The pod must be homed each time the Biomek NX instrument is powered on Add Device Installs a device Remove Device Removes a device Accept Saves all changes to the instrument and closes Hardware Setup Choose this option after the device has been installed and configured Cancel Closes Hardware Setup without saving the modifications to the instrument Biomek NX Multichannel Liquid Handler User s Manual 3 4 Configuring the Biomek NX MC in Biomek Softy 3 2 Configuring the Biomek NX Multichannel Instrument The Biomek NX Multichannel instrument is configured in Hardware Setup to relate information about the configuration of the multichannel pod on the system to the software and to specify the communications port to which it is connected To configure the Biomek NX instrument 1 From Hardware Setup select Biomek NX from the left pane The configuration view appears in the right pane Figure 3 1 2 Make sure the serial number listed in Hardware Setup corresponds to the seri
60. is 3 each time Right is clicked in the Vector Builder 3 cm is added to the X axis of the Movement Vector Note The move does not occur until Go is selected in the Movement Vector area of Advanced Manual Control To set Delta values 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 Choose Advanced Controls Select Pod1 Advanced Manual Control for the pod appears Figure 5 5 Enter a value in X Y Z and D to set the Delta value for each axis In D Units select cm or pL Choose Close to close Advanced Manual Control a A vA A U N Choose Exit to close Manual Control 5 4 5 Performing Relative Moves for the Pod Relative moves allow the pod to move from its current location to anywhere on the deck Relative moves are created in the Vector Builder using the Delta values or the vector can be manually edited Note Use Auto Clear when the values in the Movement Vector fields must be reset to zero after the move is performed Turn off Auto Clear to retain the values after the move has occurred Choose Clear to set the values back to zero at any time Biomek NX Multichannel Liquid Handler User s Manual 5 10 Manually Controlling the Biomek NX in Biomek To move a pod relative to its current position 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 5 5 4 C
61. itions on the Biomek NX can also be framed by choosing Manual Teach on Position Properties Manual Teach involves loading tips and visually aligning them with the wells on a piece of labware refer to Section 4 3 Manually Framing Deck Positions on the Biomek NX Instrument Manual Teach is useful if specialized or very high density labware is used or when Auto Teach yields unsatisfactory results Some ALPs such as the Positive Position ALP must be framed manually 4 2 1 Homing All Axes of the Pods CAUTION Before selecting OK to home all axes the following conditions must be met e Make sure there is no liquid present in the tips e Make sure there are no disposable tips loaded e Make sure the Framing Probe is NOT installed on the Multichannel Pod e Make sure the grippers on the Multichannel Pod are retracted Prior to framing the Biomek NX deck all axes must be homed Homing the pod gives Biomek NX a point of reference from which to make subsequent moves Home position is left and back While it is necessary to home the pod after the Biomek NX instrument is powered on it is not necessary to home the pod each time the host computer is turned on or the software is accessed Note Home the pod each time the Biomek NX instrument is powered on When attempting to use the pod error messages result until the pod is homed Biomek NX Multichannel Liquid Handler User s Manual 4 4 Framing Instructions To home the pod 1 C
62. k NX in Biomek Software 5 1 OVETVIEW srnedi naar Pinpin er Rak ee EEE a ARA ENERALE ETA 5 1 5 2 Accessing Manual Conttlie ccxdscGse 4d Pe eee ereere 5 2 5 3 Using Manual Control 2 450 vesreee ents eee araneae 5 3 5 3 1 Homing all Axes of the Pod o n sau wee es OSs aaa 5 3 5 3 2 Moving the Pod to a Specific Deck Position 5 4 5 3 3 Stopping Me POG 66644 seatot et asn eda saatena aiaia Ea aa 5 4 5 3 4 Viewing the Firmware Version 000 0 cece cece nee ees 5 4 5 3 5 Accessing Advanced Manual Control 0 000000 eee 5 4 5 4 Using Advanced Manual Control 0 0 nananana nerra 5 5 5 4 1 Viewing the Current Position the Pod 0 000005 5 5 5 4 2 Moving the Pod to a Safe Roving Height 0 5 8 5 4 3 Understanding Movement Vectors for the Pod 5 9 5 4 4 Setting Delta Values for the Pod ccc cisccudacceieesdeeieness 5 9 5 4 5 Performing Relative Moves for the Pod 0 0 0008 5 9 5 4 6 Performing Absolute Moves for the Pod 0 00 5 12 5 4 7 Extending and Retracting the Grippers 000005 5 13 Appendix A Specifications 0 0c ee A 1 ING OX 22 occ che ene de eee et esse eee bese Geko ee ce eee oes xix Biomek NX Multichannel Liquid Handler User s Manual List of Figures Figure 1 1 Biomek NX with Multichannel Pod cece ceecceeseeeseceeeeeeeeeeseeceaeceeeseeeeeseeesaeen
63. ltichannel head is attached to the bottom of the pod to perform a specific liquid handling procedure Depending on the head and the desired liquid handling procedure different tip types may be used Presently there are three types of heads that can be attached to the Multichannel Pod e 96 Channel 20 uL Head e 96 Channel 200 uL Head e 384 Channel 30 uL Head Interchangeable heads installed on the Multichannel Pod aspirate and dispense liquid using disposable tips The tips compatible and the maximum volume that may be aspirated and dispensed with those tips varies for each head as described in Table 2 2 Table 2 2 Tip compatibility and max volumes on Multichannel Heads b 2 ma A a 96 Channel 20 uL Head 55 uL 20 pL SSuL 50uL 96 Channel 200 uL Head 120 uL 20 uL 225 uL 125 uL 384 Channel 30 uL Head 35 uL 2 3 1 Changing Heads Each Multichannel head can be removed and replaced to accommodate the needs of a particular method Note When a head is changed the Hardware Setup must be changed appropriately If the hardware configuration is not updated using Hardware Setup inaccurate pipetting may occur refer to Section 3 3 Configuring a Multichannel Pod Biomek NX Multichannel Liquid Handler User s Manual 2 6 Multichannel Pod 2 3 1 1 Removing Heads CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist groun
64. me All Axes Get Version Click on a position to move the pod to it Figure 5 2 Manual Control ly Controlling the Biomek NX in Biomek Software 5 3 5 3 Using Manual Control Use Manual Control for Homing all Axes of the Pod Section 5 3 1 e Moving the Pod to a Specific Deck Position Section 5 3 2 e Stopping the Pod Section 5 3 3 Viewing the Firmware Version Section 5 3 4 e Accessing Advanced Manual Control Section 5 3 5 5 3 1 Homing all Axes of the Pod CAUTION Before selecting OK to home all axes the following uN conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Tool is NOT installed on the Multichannel Pod Make sure the grippers are retracted Home the pod each time the Biomek NX instrument is powered on Homing the pod gives Biomek NX a point of reference from which to make subsequent moves Home position is left back Note When attempting to use the pod error messages result until the pod is homed Note While it is necessary to home the pod after the Biomek NX instrument is powered on it is not necessary to home the pod each time the host computer is turned on or the software is accessed To home the pod 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 2 Choose Home All Axes The following Warning appears Figure 5 3 Warning x CAU
65. ms Beckman Coulter Service Engineer Biomek NX Multichannel Liquid Handler User s Manual Manually Controlling the Biomek NX in Biomek Software 5 1 Overview Manual Control and Advanced Manual Control are used to control e Movement of the pod e Grippers independently of a method e Pod when teaching the deck framing the grippers and recovering from errors e Tip Loader and other ALPs independently of a method Note Refer to the ALPs User 5 Manual for information on manually controlling specific ALPs The sections in this chapter include e Using Manual Control Section 5 3 e Using Advanced Manual Control Section 5 4 Biomek NX Multichannel Liquid Handler User s Manual 5 2 Manually Controlling the Biomek NX in Biomek Sot 5 2 Accessing Manual Control Beckman Coulter Inc To open Manual Control choose Instrument gt Manual Control An Information dialog Figure 5 1 appears briefly as the connection is made with the Biomek NX instrument immediately followed by Manual Control Figure 5 2 Note Manual Control is available only when a method is not being executed If a need for manual control is realized during a method run stop the method using the Stop button or Snap Continuation button refer to the Biomek Software User 5 Manual Chapter 26 11 2 Snapping a Continuation Information x G Connecting Manual Control lt SS FA Advanced Controls Ho
66. n along the front edge of the Biomek NX instrument is a standard component Note Side safety shields are installed by a Beckman Coulter Service Engineer when the Biomek NX instrument is installed The light curtain projects a diffused array of infrared light rather than a vertical laser like beam Figure 1 5 When a part of the human body or an object larger than approximately 1 in diameter such as labware and large cables penetrates this protective zone the instrument shuts down immediately stopping all pod and head operations Some ALP operations such as shaking or stirring continue ALPs respond to a violation according to safety and operational requirements specific to each ALP For example a refilling reservoir may continue to operate if user safety is not compromised ALPs that operate with a motion that could present a danger to the operator go to a safe state when the light curtain is violated Note When active ALPs or optional devices are operating and the light curtain is violated an error message may not appear until after the ALP or optional device operation is complete Note It is important to become familiar with this protected zone This reduces the possibility of causing the instrument to shut down accidentally during operation by unintentionally violating the light curtain zone When the instrument is sitting idle or in the paused mode no violations are registered when the protective zone is penetrated This all
67. n charges collect unless the product is found to be defective in which case the product must be properly decontaminated of any chemical biological or radioactive hazardous material d The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company e Ifthe product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement Representatives and warranties made by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company Parts replaced during the warranty period are warranted to the end of the instrument wa
68. n of the pod is not changed between the time the vector is built and the time the Go button is pressed 1 Choose Instrument gt Manual Control Manual Control appears Figure 5 2 Choose Advanced Controls Select Pod1 Advanced Manual Control for the pod appears Figure 5 5 A WwW N Choose Absolute Move Absolute Move appears Figure 5 7 Absolute Move M Curent Position x 1 092 cm Y f 0 1 em Z 3 95 em D 219 379 cm Enter the absolute coordinates to move to cm 0 1 em Z 3 95 cm D A 9 379 uL These values will be converted to the necessary relative move numbers on the Advanced Manual Control form when you click OK Then you can press Go to move to these coordinates Cancel Figure 5 7 Enter Absolute Move Coordinates 5 Enter the X Y Z and D values for the desired position Note Minimum and Maximum X Y Z and D values displayed in Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors 6 Choose OK The Movement Vector changes to reflect the necessary relative move 7 Enter a value in Speed to specify the percent of the pod s maximum speed to use for the move 8 Choose Go The pod moves from its current position to a specified absolute position The new position is displayed in Current Position 9 Choose Close to close Advanced Manual Control 10 Choose Exit to close Manual Cont
69. n the pod returns to along the D axis after loading tips Final D Position under Tip Unload Settings Position the pod returns to along the D axis after unloading tips Gripper Extend Time Specifies the time the pod takes to extend grippers Gripper Retract Time Specifies the time the pod takes to retract grippers Biomek NX Multichannel Liquid Handler User s Manual 3 8 Configuring the Biomek NX MC in Biomek Soft Beckman Coulter Inc Table 3 2 Multichannel Pod Properties Continued Property Gripper X Y Z D Offsets Description Controls gripping operation along X Y Z and D axes from center of back edge of deck position These offsets are automatically updated when the grippers are framed Note Gripper X offset is read only Last Validation Set by a Beckman Coulter Service Engineer using the Set Validation button Maximum X Y Z D The furthest position the pod may move to along the X Y Z and D axes relative to the Home position Set by using the appropriate buttons under the X cm Y cm Z cm and D uL columns The D axis must be established when a head is changed but do not change the other axes limits without contacting a Beckman Coulter Service Engineer Minimum X Y Z D The closest position the pod may move to along the X Y Z and D axes relative to the Home position Set by using the appropriate buttons under
70. ndrels Note Make sure the front of the framing fixture is to the front of the instrument and the framing guides are to the back and left of the instrument Figure 4 3 2 Gently pull the framing fixture to the front and right Verify the framing guides are touching the mandrels 3 Tighten each thumb screw by turning to the right until the framing fixture is firmly attached to the head The pod is now ready for framing Back of framing fixture Front of framing fixture Framing probe SAREE J Thumbscrews Four thumb screws attach the framing fixture to the Multichannel head Figure 4 3 Framing Fixture 4 Install AccuFrame in the position to frame refer to Section 4 2 3 Installing AccuFrame and frame the Multichannel Pod according to the procedure in Section 4 2 4 Framing the Position Biomek NX Multichannel Liquid Handler User s Manual 4 6 Framing Instructions gt A Beckman Coulter Inc 4 2 3 Installing AccuFrame AccuFrame is used during the framing process to frame deck positions After the framing shaft is attached to the pod and AccuFrame is positioned on the ALP the framing process is completed through the software WARNING Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck 1 Turn off power to the Biomek NX instrument before connecting the Acc
71. nsation Total Moved From Start cm 0 0 0 Reset C Well Comer well Center Go Finish Figure 4 14 Framing to the center of a well Biomek NX Multichannel Liquid Handler User s Manual 4 16 Framing Instructions 11 To align the tips in the X and Y axes with the wells of the microplate on top of the ALP lower the tips in the Z axis until they are approximately 1 mm above the top of the ALP Note Since tip height is set in the next step in the Manual Teaching process it is safe to move the pod to any height to make aligning the tips with the microplate easier 12 Visually verify the physical position of the tips in relation to the physical position of the wells of the microplate on top of the ALP 13 Adjust the position of the tip to align it with the microplate well or corner by either e Using the Graphic Alignment Tool Section 4 3 1 Using the Delta Value and Directional Buttons Section 4 3 2 4 3 1 Using the Graphic Alignment Tool The graphic alignment tool Figure 4 15 is used to instruct Biomek Software of the current position of the tips to the physical position of the wells of the microplate on top of the ALP Biomek Software uses the information it is given to move the pod so the tips are directly above the target position W Biomek NX Manual Framing Wizard E 0 x The tips should be about a half centimeter above the plate Drag the circle to where th
72. nt adwakbeehad des 1 10 Preventive Maintenance nunnu eae wae noe we oa eee ep alee eS 1 12 2 Multichannel Pod 2 1 2 2 2 3 2 4 2 5 2 6 3 1 OVETVIEW srca sats i epua eE EE ha PETEA Oe Reed TEREE ECE ERR 2 1 2 1 1 Main Components of the Multichannel Pod 2 3 laa PEE EEEE ee eee T ee EEE T E EE 2 4 2 2 1 Pod Movements 4 5 pV a see es amiert ig reia a ea 2 4 222 2 Control Modes css dg horse ae 4S ded ORD aR REINS Hon eRe ni 2 4 Interchangeable Heads vis 4 626 tnand exidaes tert EET E 8s EEN EEEIEE EN 2 5 2 3 1 Changing Heads nnan nananana 2 5 GTIPpeTS resem coated REET ew EREET EE 4 tea ad Bed ERAR EREE E ET ea de 2 12 2 4 1 Framing Grippers vows dent oer ediweathuwtotateandonse ddan 2 13 Preventive Maintenance ca ica oc tGawniaeiednns CabR dee adedawbs ands 2 14 Troubleshooting 4 pueen hoe dec aa ease eee n nira GE En Edra ET Rees 2 15 2 6 1 Replacing Grippers a en ess era ops ad oe ed pense kw Pe de es 2 16 3 Configuring the Biomek NX MC in Biomek Software Ovt VIEW 5 bebe do eins Ek ek kd DEERE Ae CREATEED ECLER ORRERA 3 1 3 1 1 Accessing Hardware Setup ive vuvesdanes eo ku kde ead cua vers 3 2 3 1 2 Understanding the Options in Hardware Setup 3 3 3 2 3 3 3 4 4 1 4 2 4 3 4 4 Configuring the Biomek NX Multichannel Instrument3 4 Configuring a Multichannel Pod 2 0 0c eee eee 3 5 3 3 1 Configuring a New Head 004460 4a 6 eedvan b
73. odestaces ted deces 3 5 3 3 2 Setting Multichannel Pod Properties 00 0000 e eae 3 7 Saving Restoring and Deleting Settings 0 00 c cece eee 3 10 3 4 1 Saving Pod Seuss cae head atts seats eee taeda eee eee 3 10 3 42 Loading Pod Settings 4 lt 524 04b00s 46264 ssn Err ean EE EEE y 3 11 3 4 3 Deleting Pod Settings n annann nnn nanara 3 12 4 Framing Instructions OVERVIEW coccia rin EE NERC EE COREEA ARREARS EREE EENE EA AAEE HAGE REA 4 1 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 4 2 4 2 1 Homing All Axes of the Pods 0 cece cece eee eee 4 3 4 2 2 Attaching the Framing Fixture to the Pod 0005 4 5 4 2 3 Installing AccuFrame 4 lt 5 026454 p00ths adh iasod seed coeds neces 4 6 424 Framing the Positions 4ciai icacagenaacdesacidaatiaasiwndes 4 7 Manually Framing Deck Positions on the Biomek NX Instrument 4 11 4 3 1 Using the Graphic Alignment Tool 000000000 ee 4 16 4 3 2 Using the Delta Value and Directional Buttons 4 18 4 3 3 Framing the Z Axis on cae np weaeie a Re Nee wae ae eR ER ee RES 4 20 Framing the Grippers 26 2iaeds as tak ewes Shae Eek E eed eh AEM aRE RS 4 22 4 4 1 Testing Gripper Framing Accuracy 0000 eee ee eee 4 26 NtGubleshOOUNe 246i ccc einsaadaderba ede Rnnte aca ened ied EREA EE 4 27 4 5 Beckman Coulter Inc Table of Contents xiii 5 Manually Controlling the Biome
74. of Manual Framing Wizard a list of steps required to complete the teaching process is displayed As the steps of Manual Framing Wizard are accessed the steps are highlighted on the left W Biomek NX Manual Framing Wizard E Warning Warning If you have snapped a continuation manually framing will erase it Press Cancel now to avoid this Clear all other positions of labware and then press Next Figure 4 10 Manual Framing Wizard Warning Framing Instructions 4 13 6 Choose Next and Manual Framing Wizard Setup appears Figure 4 11 or Figure 4 12 appears depending on whether or not tips are loaded onto the pod E Biomek NX Manual Framing Wizard Load tips from the v tipbox on position Tu ba Warning Line tips up against 4B384 WellReactionPlate on position P10 Finish cee Laer Figure 4 11 Manual Framing Wizard if tips are not already loaded lo x E Biomek NX Manual Framing Wizard Use currently loaded tips on position P10 Warning Line tips up against Finish cont Laer Figure 4 12 Manual Framing Wizard if tips are loaded 7 Ifno tips are loaded in Load tips from the select the type of tips to load and the position on which the tip box is located Figure 4 11 8 In Line tips up against select the appropriate labware type of t
75. or the Multichannel Pod ssnsssssssessesessseessesssessseeseese 5 11 Table A 1 System SPSCrhi Cations 2 ncncescovsatcaceanasdccasedetahenecensccsasnenseasnessaiensetaceesaiseatanensesiovessan ss A 1 Biomek NX Multichannel Liquid Handler User s Manual Biomek NX Multichannel Instrument Introduction 1 1 Instrument Overview The Biomek NX Multichannel liquid handler is a multiaxis liquid handling instrument used in the drug discovery laboratory The open architecture design along with the extensible operating software provides a foundation for integrating current and future specific use components The design of the Biomek NX provides the foundation for continual evolution and expansion of liquid handling systems The Biomek NX Multichannel is equipped with a Multichannel Pod with a pair of grippers for grasping and moving labware Figure 1 1 A variety of labware and hardware adapt the deck of the Biomek NX Multichannel to accomplish multiple tasks ranging from performing simple labware positioning and liquid transfers to completing complex activities that typically require additional devices in the laboratory Note For detailed information on the Multichannel Pod and interchangeable heads refer to Chapter 2 Multichannel Pod The capabilities of the Biomek NX Multichannel liquid handler depend on the components installed including the pod configuration ALPs and third party devices 1 1 1 Control Modes
76. ows full access to instrument components ALPs and labware on the Biomek NX deck during a pause or system idle time 1 2 7 2 Side Safety Shields WARNING To reduce the risk of personal injury operate the Biomek NX VAN instrument only with all protective shields in place Side safety shields are installed by the Beckman Coulter Service Engineer on the Biomek NX instrument If the instrument overhangs the table work surface on either side a protective bottom shield is also installed on the side of the instrument overhanging the instrument envelope Beckman Coulter Inc 1 3 2k NX Multichannel Instrument Introduction 1 9 Troubleshooting A CAUTION If service is required contact a Beckman Coulter Service Engineer Perform the troubleshooting techniques provided in Table 1 1 when necessary In the case of any other instrument related problems contact a Beckman Coulter Service Engineer Note For troubleshooting information regarding the specific pods refer to the Troubleshooting section in those respective chapters Table 1 1 Troubleshooting the Biomek NX Instrument If Then All indicator lights are out Check fuses The power is on but system does not Check fuses run The fuses appear burned or filaments Change fuses refer to Section 1 3 1 are broken Changing Fuses All indicator lights are out the power Contact a Beckman Coulter Service is on the fuses are okay and the Enginee
77. panel Check to make sure the solid amber indicator light changes to blinking amber each time the light curtain is violated If not contact a Beckman Coulter Service Engineer e When necessary clean light curtain panels with a lint free cloth e Once every 2 3 months clean light curtain panels with a non abrasive cleaner making sure not to scratch the strip Beckman Coulter Inc Multichannel Pod 2 1 Overview The Multichannel Pod is a self contained working unit on the Biomek NX Multichannel liquid handler Figure 2 1 The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions refer to Section 2 3 Interchangeable Heads The Multichannel Pod contains its own electrical cabling communication and pneumatic connections with the base unit and it interacts with ALPs located over the entire deck area of the Biomek NX Biomek NX Multichannel Liquid Handler User s Manual 2 2 Multichannel Pod Figure 2 1 Multichannel Pod installed on Biomek NX Beckman Coulter Inc Multichannel Pod 2 3 2 1 1 Main Components of the Multichannel Pod The main components of the Multichannel Pod are Figure 2 2 e Pod Houses operating mechanism pneumatic air line communication and electrical power connections to the base unit and moves in the Y Z and D axes for liquid handling functions refer to Section 2
78. r system does not run Power is lost to pod Contact a Beckman Coulter Service Engineer Y axis motion is choppy Contact a Beckman Coulter Service Engineer A grinding or growling noise is heard Contact a Beckman Coulter Service Engineer Experiencing problems relating to the Refer to Section 2 6 Troubleshooting Multichannel Pod in Chapter 2 Multichannel Pod for more information Constant light curtain errors even Contact your Beckman Coulter when no violation Service Engineer Biomek NX Multichannel Liquid Handler User s Manual 1 10 Biomek NX Multichannel Instrument Introduct A A Beckman Coulter Inc 1 3 1 Changing Fuses WARNING Do not remove tower covers to access electrical wiring or fuses Change only the fuses that are accessed from the outside of the instrument without removing covers Contact a Beckman Coulter Service Engineer if further access is required WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage The Biomek NX instrument uses any AC power source between 100V and 240V The fuse holder holds two European fuses and one North American fuse Fuses are located in the outside right tower Figure 1 6 If power is lost check the fuses that are accessed from the outside of the instrument If fuses appear burned change them by completing the following 1 Turn off the instrum
79. r 1 6 light curtain 1 8 protective side safety shields 1 8 bridge overview 1 5 C control modes for the Multichannel Pod 2 4 overview 1 1 D deck overview 1 6 Delta defined 5 6 setting values for the Multichannel Pod 5 9 delta values using in Manual Teach 4 18 Device Controller overview 1 6 devices optional overview 1 6 F firmware version viewing 5 4 Biomek NX Multichannel Liquid Handler User s Manual framing using delta value 4 18 using graphic alignment tool 4 16 framing teaching grippers 4 22 instructions 4 1 using Manual Teach 4 11 framing fixture attaching to the head of Multichannel Head 4 5 fuses changing in tower 1 10 G graphic alignment tool using during framing 4 16 grippers defined 2 14 extending and retracting 5 13 framing 2 13 framing teaching 4 22 removing 2 17 testing framing accuracy 4 26 troubleshooting 2 15 H Hardware Setup accessing 3 2 choosing Frame Gripper 4 23 configuring a Multichannel Pod in 3 5 configuring a new head in 3 5 configuring the Biomek NX in 3 4 defined 3 1 Multichannel Pod properties in 3 7 saving restoring and deleting settings in 3 10 understanding the options in 3 3 using Accept in 3 3 heads changing 2 5 installing 2 9 removing 2 7 homing all axes of a pod 5 3 pod 4 4 Beckman Coulter Inc M Manual Control accessing 5 2 defined 5 1 dialog display 5 1 5 2 using 5 2 Manual Teach delta value 4 18 directional bu
80. re version information ics suscaivdescsascsasscotyandencesaaadscabeiavecainea dh snasaanuaetasonssantaanys 5 4 Figure 5 5 Overview Advanced Manual Control for a Multichannel Pod See Table 5 1 for further descriptions of fields ee eeeeceesseceteeeeeeeeeeeesees 5 6 Figure 5 6 Vector ALE esata yc acccs ag ccacss cece a stem ce ee 5 10 Figure 5 7 Enter Absolute Move Coordinates cccccecsceesseeseceeeeeecceeseeceaeceeeeeeeessaecsaeeneenaes 5 12 Beckman Coulter Inc xvii List of Tables Table 1 1 Troubleshooting the Biomek NX Instrument ccc ccccesceseeesseceeceeeeeeeeeeseeenaeees 1 9 Table 2 1 Multichannel Pod Axes Movement s scsiistssutsasivvscxueritonsesdenncivunsinrtanctiendecatuniesuaessenis 2 4 Table 2 2 Tip compatibility and max volumes on Multichannel Heads ceeeeeeneeseereeeee 2 5 Table 2 3 Multichannel Pod Troubleshooting siscs csiieassxaccaiasesecnssaainaiaenneosereaaneee 2 15 Table 3 1 Hardware Setup Options lt 5 ascecssccetsaincanasctabencestanencunsadeacencntasesstntentantunexeesenaraconendeateses 3 3 Table 3 2 Multichannel Pod Properties ssi ssecssoccxsesainsaceisssacatesasctaianepincvsasioeesniesacarsohaatesoassacdbadns 3 7 Table 4 1 Troubleshooting Framing acssasssnucsaawooeucnddesesesasnancastssateasscxcsusudsesuadssosenisdseinessvbeissetees 4 27 Table 5 1 Advanced Manual Control selection areas for the Multichannel Pod 00 5 7 Table 5 2 Vector Builder Buttons f
81. rol Beckman Coulter Inc y Controlling the Biomek NX in Biomek Software 5 13 5 4 7 Extending and Retracting the Grippers Use Advanced Manual Control to extend or retract the grippers when changing the head on the pod CAUTION Select Move Z Max to move the pod to its highest point before extending the grippers To avoid breaking labware or bending the grippers make sure the grippers will not hit any labware when extended CAUTION Do not retract grippers when they are holding labware gt gt From Advanced Manual Control for the pod e Choose Extend Gripper to extend the grippers Choose Retract Gripper to retract the grippers Biomek NX Multichannel Liquid Handler User s Manual Appendix A Specifications Table A 1 System Specifications Item Description Environment Indoor use only Power Requirements US 100 120VAC 60Hz Europe 200 240VAC 50Hz Pneumatic Requirements 40 100 psi of clean dry air 275 690 kPa of clean dry air Compressed Air Air Pressure 40 100 psi of clean dry air Requirements 414 690 kPa of clean dry air Air Flow 2 25 cfm 40 psi 0 064 m min 414 kPa Dimensions of base unit 36 in L x 31 in W x 44 in H 91 44 cm L x 78 74 cm W x 86 36 cm H Weight 180 Ibs includes instrument and tip loader Ambient Operating 5 30 C 59 86 F Temperature Humidity Restrictions lt 85 non condensing 30 C 86 F Altitud
82. rranty Note Performance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used Biomek NX Multichannel Liquid Handler User s Manual Safety Information All Warnings and Cautions in this document include an exclamation point a lightning bolt or a light burst symbol framed within a triangle Please pay special attention to the specific safety information associated with these symbols Warning and Caution Definitions The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing is attempted When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol WARNING A WARNING calls attention to a condition or possible situation that could cause injury to the operator CAUTION A CAUTION calls attention to a condition or possible situation that could damage or destroy the product or the operator s work Biomek NX Multichannel Liquid Handler User s Manual vi Safety Information Electrical Safety A Laser Light A Beckman Coulter Inc To prevent electrically related injuries and property damage properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies Contact an Beckman Coulter service representative for any serv
83. rs locate each position reliably There are three indicator lights on the AccuFrame e First light indicates that the AccuFrame is powered on e Middle light indicates the framing status of the Y axis e Third light indicates the framing status of the X and Z axes Note The AccuFrame is calibrated by a Beckman Coulter Service Engineer to one instrument only and each AccuFrame is assigned a unique identification number This number is stored in Biomek Software and the AccuFrame Each Biomek NX must be framed with the AccuFrame assigned to that instrument Contact a Beckman Coulter Service Engineer to calibrate AccuFrame to multiple instruments Beckman Coulter Inc Framing Instructions 4 3 To frame deck positions of the Biomek NX using AccuFrame a framing fixture is attached to the head of the Multichannel Pod When framing multiple position ALPs such as the 1 X 3 Passive ALP all positions on the ALP should be framed to increase precision To frame the deck positions of the Biomek NX the following operations must be completed Homing All Axes of the Pods Section 4 2 1 Attaching the Framing Fixture to the Pod Section 4 2 2 e Installing AccuFrame Section 4 2 3 Framing the Position Section 4 2 4 Note Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User s Manual for the specific ALP to frame for any special instructions Note Deck pos
84. s 1 2 Fig rt 1 2 Left outside TO WEE sirrane na eeri e E E E EELE ERE REE 1 4 Figure 1 3 Right tower inside x assicaedesueseastaysnastenGascastacen ates seeseatiaconnteecacunentaeins ca tecanaderauescasianess 1 5 Fig re J4 Right tower Outside seais r a 1 5 Figure 1 5 Protective barriers for the Biomek NX Instrument esssssessessesensessesesssrsrrseseesesse 1 7 Figure 1 6 Replacing fuses in TOWEL s cssscsabesenrsader doscassecincsenieanderanasnsaniantese MiacuaeGeesanonens 1 11 Figure 2 1 Multichannel Pod installed on Biomek NX 00 e ce eeceeeeeeeseeeeeeeeceseceaeceeeeaeeeeeeeeneees 2 2 Figure 2 2 Multichannel Pod main componenttS cccsscssseesesseeceeeeeeceeseccesecesceseeseeeneeees 2 3 Figure 2 3 Wrist ground strap attached to the Weeki cicccavseasntecrermiennavid tannins 2 6 Figure 2 4 Bottom cover removed from the pod ccccesessesseeseeseeseceeceaeceeeaeeeeeeeeeeaeenaeeneeees 2 7 Figure 2 5 Plunger screws and shoulder screws removed from multichannel head 2 8 Figure 2 6 Sequence for removing four plunger screws and four shoulder screws on all M ltichanpel WAS sissies nnie a an RASE 2 9 Figure 2 7 Chamfer location on a Multichannel head 00 eee eceeceeeeceneceeeesecneeeseeeeeeeeeeaeenaes 2 10 Figure 2 8 Sequence for installing four plunger screws and four shoulder screws on all M l channel Nedds srera n E a Ea EEE nee 2 10 Figure 2 9 GPPElS y ccrcesc psec caniedaecenn
85. s Manual 4 20 Framing Instructions 4 3 3 Framing the Z Axis After the X and Y axes are framed the Z axis must be framed to ensure aspirate and dispense operations are performed at the desired height The final screen in Biomek NX Manual Framing Wizard is for framing the Z axis Figure 4 17 1 Beckman Coulter Inc W Biomek NX Manual Framing Wizard 0 x The tips should be 0 5 centimeters above the well bottom Lower the pod until the tips just touch the bottoms of the wells until the plate does not move when lifted then click Finish Warning Setup Delta foos 4 cm TeachxX Y E Back Left gt Bight Teach Z Ewd Down J Hysteresis compensation Total Moved From Start cm l Finish e tor Figure 4 17 Manual Teaching Teach Z In Delta select the magnitude of change applied to the pod each time a directional button is selected Figure 4 17 Note The default Delta value is 0 05 cm If the tip is a considerable distance above the position increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tip is almost to the desired location reduce the Delta value minimum setting is 0 005 cm Note Since the X and Y axes were framed previously it is safe to move the pod in the X and Y axes if it makes framing the pod in the Z axis easier Framing Instructions 4 21 2 Select the directional button repr
86. s above the wells of the microplate on top of the ALP Delta value The magnitude of change applied to the pod each time a directional button is selected E Biomek NX Manual Framing Wizard The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target Warning Deta 005 fom ft E Back Left gt Bight Teach XY mes ee Down IV Hysteresis compensation Total Moved From Start cm Well Comer Well Center Go Ead Directional buttons The directional buttons move the pod by the amount shown in Delta with each press of a button e ter Figure 4 16 Manual Teaching the X and Y axes To use the delta value and directional buttons to position the tip 1 In Delta select the magnitude of change applied to the tips each time a directional button is selected Figure 4 16 Note The default Delta value is 0 05 cm If the tips are a considerable distance from the desired location increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tips are almost to the desired location reduce the Delta value to fine tune the position minimum setting is 0 005 cm Beckman Coulter Inc Framing Instructions 4 19 2 Select the directional button representing the mo
87. the X cm Y cm Z cm and D uL columns The D axis must be established when a head is changed but do not change the other axes limits without contacting a Beckman Coulter Service Engineer Speed Limit Controls the speed for pod movement based on a percent of its maximum speed Tip Settle Time Specifies the time required for tips to settle on the mandrels after loading Tip Unload Time Specifies the amount of time the pod takes to unload tips Unload Speed Controls the pod speed when unloading tips based on a percentage of the maximum pod speed Recommended that this be modified only by a Beckman Coulter Service Engineer uring the Biomek NX MC in Biomek Software 3 9 3 3 2 1 A plate sensor is available on the grippers When the grippers have a plate sensor and Enable Plate Sensor Figure 3 4 is checked in Hardware Setup or for the specific labware in the Labware Type Editor labware can be sensed when the grippers are squeezed Using the Plate Sensor An error message Figure 3 5 appears if labware is not sensed when the grippers are squeezed Refer to the Biomek Software User s Manual Chapter 26 Handling and Preventing Errors for information on recovering from errors Note By default Enable Plate Sensor is enabled Biomek Hardware Setup 5 E Reconnect ft Home All Axes qb Add Device me Remove Device JY Accept gt Cancel Serial Number Nong
88. tion required to physically move the tips into position over the wells of the microplate on top of the ALP Figure 4 16 Note Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction Note The tips can be physically positioned over the wells of a microplate using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 3 Visually verify the position of the tips on the pod in relation to the physical position of the wells of the microplate on top of the ALP If the tips are still not accurately positioned above the wells of the microplate on top of the ALP repeat steps and 2 until they are accurately positioned above the target position Note The tips may also be aligned with the target position using the graphic alignment tool refer to Section 4 3 1 Using the Graphic Alignment Tool 4 Once the tips are aligned with the target position choose Next to continue Biomek NX Manual Framing Wizard displays Teach Z refer to Section 4 3 3 Framing the Z Axis Biomek NX Multichannel Liquid Handler User
89. tower e Electrical plug ins and CAN connections for active ALPs on the inside of the right tower and outside of both towers Biomek NX Multichannel Liquid Handler User s Manual 1 4 Biomek NX Multichannel Instrument Introducti Pneumatically regulated air supply ports outside of the left tower for active ALPs Main power fuses for the instrument on the outside of the right tower Q AIR OUT MIN 60 psi 414 kPa 40 psi 110 psi MAX 275 kPa 758 kPa 100 psi 690 kPa AB ABS CAN PORT CAN PORT Figure 1 2 Left outside tower Beckman Coulter Inc iomek NX Multichannel Instrument Introduction 1 5 CAN PORT Figure 1 3 Right tower inside Figure 1 4 Right tower outside 1 2 2 Multichannel Pod The Multichannel Pod is a self contained component and holds various removable and interchangeable heads such as the 96 Channel 200 uL Head that perform liquid handling operations and a pair of grippers for labware movement operations Refer to Chapter 2 Multichannel Pod for more information on the Multichannel Pod The operation of the pod and probes are controlled by Biomek Software from the host computer Biomek NX Multichannel Liquid Handler User s Manual 1 6 Beckman Coulter Inc Biomek NX Multichannel Instrument Introc 1 2 3 Deck The deck Figure 1 1 is the worksurface of the Biomek NX instrument The deck is laser etched with standard deck positions T
90. ttons 4 18 framing Z axis 4 20 using 4 11 using delta values 4 18 using graphic alignment tool 4 16 Move Z Max using in Advanced Manual Control for a Multichannel Pod 5 8 movement vectors understanding for a Multichannel Pod 5 9 Multichannel Pod control modes 2 4 current position coordinates 5 5 defined 2 1 immediate moves for 5 6 main components 2 3 grippers 2 3 head 2 3 pod 2 3 movement values defined 5 9 movements 2 4 moving to a safe roving height 5 8 performing absolute moves for 5 12 performing relative moves 5 9 wrist ground strap changing heads 2 6 displayed as O open architecture instrument design concept 1 1 P pod framing teaching Multichannel 4 3 Span 8 4 3 manually controlling 5 3 moving to a specific deck position 5 4 Multichannel defined 2 1 3 5 stopping in Manual Control 5 4 teaching framing Multichannel 4 3 Span 8 4 3 Pod Editor properties defined 3 7 S settings saving restoring and deleting in Hardware Setup 3 10 specifications Biomek NX system A 1 T teaching framing grippers 4 22 instructions 4 1 using Manual Teach 4 11 tower changing fuses in 1 10 troubleshooting 1 9 V Vector Builder choices defined 5 11 defined 5 6 using 5 10 W wrist ground strap changing heads 2 6 Biomek NX Multichannel Liquid Handler User s Manual
91. uFrame 2 Plug AccuFrame into an available CAN port on the Biomek NX tower WARNING Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately WARNING Make sure the AccuFrame cable does not interfere with pod movement 3 Turn on power to the Biomek NX instrument 4 Manually place the AccuFrame onto the ALP position that requires framing by placing the back right corner first and pushing the AccuFrame gently down onto the ALP position Note The deck is usually framed from from left to right starting at the back left position However ALPs may be framed in any order 5 Make sure that the AccuFrame is fully seated along the posts along the top and right sides of the position Note Some ALPs require a framing adaptor to accommodate using AccuFrame to frame the position Make sure the correct adaptor is used for each type of ALP Framing proceeds as normal once the AccuFrame is placed on the appropriate adaptor Framing Instructions 4 7 4 2 4 Framing the Position To frame the Biomek NX deck position Note Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User 5 Manual for the specific ALP to frame for any special framing instructions 1 In Biomek Software choose Instrument gt Deck Editor Deck Editor appears Figure 4 4 Deck1 Default Deck x a Ej New Deck D
92. ual regardless of foreseeability or the form of action whether in contract tort including negligence breach of warranty strict liability or otherwise and including but not limited to damages resulting from loss of data loss of anticipated profits or any special indirect incidental or consequential damages In no event shall SAGIAN s liability to the user exceed the amount paid by the user to SAGIAN hereunder The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results Beckman Coulter SAGIAN Operations Indianapolis Indiana SILAS is a trademark and SAMI and ORCA are registered trademarks of Beckman Coulter Inc Microsoft and MS DOS are registered trademarks and Windows is a trademark of Microsoft Corporation All other trademarks appearing in this manual are owned by their respective companies Copyright 2004 by Beckman Coulter Inc All rights reserved No part of this publication may be reproduced distributed or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise or stored in a database or retrieval system without the prior written permission of SAGIAN Beckman Coulter Inc Warranty and Returned Goods Requirements All standard Beckman Coulter Inc policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Company w
93. ves do not degrade light curtain operation however it is advisable to test the impact of the all lab dress on light curtain sensitivity before operating the Biomek NX Verify lab dress impact on light curtain sensitivity as follows Use Manual Control in Biomek Software and insert the material no more than 1 past and approximately 21 above the light curtain panel Make sure the solid amber indicator light changes to blinking amber WARNING To reduce the risk of personal injury operate the Biomek NX instrument only with all protective shields in place CAUTION If service is required contact a Beckman Coulter Service Engineer WARNING Do not remove tower covers to access electrical wiring or fuses Change only the fuses that are accessed from the outside of the instrument without removing covers Contact a Beckman Coulter Service Engineer if further access is required WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage WARNING To prevent injury use proper decontamination procedures CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when removing a multichannel head CAUTION Before removing the fourth shoulder screw take hold of the head firmly to make sure it does not fall once all screws are removed CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground
94. with the instructions outlined in this manual and take all the necessary precautions when using pathological toxic or radioactive materials e Splashing of liquids may occur therefore take appropriate safety precautions such as using safety glasses and wearing protective clothing when working with potentially hazardous liquids e Use an appropriately contained environment when using hazardous materials e Observe the appropriate cautionary procedures as defined by your safety officer when using flammable solvents in or near a powered up instrument e Observe the appropriate cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials Note Observe all warnings and cautions listed for any external devices attached or used during operation of the Biomek NX liquid handler Refer to applicable external device user s manuals for operating procedures of that device Biomek NX Multichannel Liquid Handler User s Manual viii Safety Information Moving Parts Cleaning Maintenance Q Important gt Beckman Coulter Inc To avoid injury due to moving parts observe the following e Never attempt to exchange labware reagents or tools while the instrument is operating e Never attempt to physically restrict any of the moving components of the Biomek NX liquid handler e Keep the Biomek NX liquid handler work area clear to prevent obstruction of the movement O

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