MLA-80 ROTOR
Contents
1. Calculated for all Beckman Coulter preparative rotors as a measure of the rotor s relative efficiency in pelleting sample in water at 20 C Relative Centrifugal Fields RCF for the MLA 80 Rotor 450 000 400 000 350 000 300 000 250 000 200 000 max av RCF x g min 150 000 100 000 50 000 0 0 10 000 20 000 30 000 40 000 Speed rpm 50 000 60 000 70 000 80 000 MLA SO Rotor 11 MLA S80 Rotor 1 90 1 85 1 80 1 75 1 70 1 65 1 60 1 55 ES SS 1 50 1 45 iomogeneous CsCl Solution g mL 1 40 1 35 1 30 1 25 1 20 MLA 80 ROTOR 10 20 25 30 35 40 45 50 55 60 65 70 75 80 Rotor Speed K rpm Figure 2 Precipitation Curves for the MLA 80 Rotor Using combinations of rotor speeds and2. see Table 1 Quick Seal Cordless Tube Topper kit 60 HZ 358312 Quick Seal Cordless Tube Topper kit 50 Hz Europe 358313 Quick Seal Cordless Tube Topper kit 50 Hz Great Britain 358314 Quick Seal Cordless Tube Topper kit 50 Hz Australia 358315 Quick Seal Cordless Tube Topper kit 60 Hz Canada 367803 Tube Topper rack ss AER een eut ae 348123 Floating spacer removal tool 338765 Tube removal tool 361668 Curved hemostat 6 in 927208 Straight hemostat 6 in 961519 Spinkote lubricant 2 0Z 306812 Silicone vacuum grease 1 0Z 335148 Rotor Cleaning Kit 339558 Rotor cleaning brush 347404 Beckman Solution 555 1 qt 339555 19 ULTRACENTRIFUGE ROTOR WARRANTY All Beckman Coulter ultracentrifuge Fixed Angle Vertical Tube Near Vertical Tube Swinging Bucket and Airfuge rotors are warranted against defects in materials or workmanship for the time periods indicated below subject to the Warranty Conditions stated below Preparative Ultracentrifuge Rotors 5 years No Proration Analytical Ultracentrifuge Rotors 5 years No Proration ML and TL
3. 10 min Approximate deceleration time from maximum speed fully loaded 3 min Weight of fully loaded rotor 2 06 kg 4 54 Ib Rotor materials issus des daube titanium Relative Centrifugal Field RCE is the ratio of the centrifugal acceleration at a specified radius and speed r amp to the standard acceleration of gravity g according to the following formula RCF DO D where r is the radius in millimeters o is the angular velocity in radians per second 2 m RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution 2 RCF 1 12r al 1000 MLA S80 Rotor DESCRIPTION Lid 367089 Lid O ring Fluid 892292 Containment Annulus Rotor Body This Beckman Coulter rotor has been manufactured in an NSAI registered ISO 9001 or 9002 facility for use with the appropriately classified Beckman Coulter ultracentrifuge The MLA 80 fixed angle rotor rated for 80 000 rpm has a tube angle of 26 degrees from the axis of rotation The rotor can centrifuge up to eight tubes and is used in Beckman Coulter Optima MAX and MAX E tabletop ultracentrifuges The rotor is made of titanium and is finished with black polyurethane paint The lid is made of aluminum and anodized to resist corrosion A rotor retention mechanism on the ultracentrifuge drive hub secures the rotor during the run A lubricated O ring made of
4. 13 MLA 80 Rotor SELECTING CsCl GRADIENTS Precipitation during centrifugation would alter density distribution and this would change the position of the sample bands Curves in Figures 2 and 3 are provided up to the maximum rated speed of the rotor I gt NOTE The curves in Figures 2 and 3 are for solutions of CsCl salt dissolved in distilled water only If other salts are present in significant concentra tions the overall CsCl concentration may need to be reduced Rotor speed is used to control the slope of a CsCl density gradient and must be limited so that CsCl precipitation is avoided Speed and density combinations that intersect on or below the curves in Figure 2 ensure that CsCl will not precipitate during centrifugation in the MLA 80 rotor Curves are provided at two temperatures 20 C black curves and 4 C gray curves The reference curves in Figure 3 show gradient distribution at equi librium Each curve in Figure 3 is within the density limits allowed for the MLA 80 rotor each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities one for each fill level that avoid precipitation at that speed The gradients in Figure 3 can be generated from step or linear gradients or from homogeneous solutions But the total amount of CsCl in solu tion must be equivalent to a homogeneous solution corresponding to the concentrations specified in Figure 2 Figure 3 can also be us
5. Buna N rubber maintains atmospheric pressure inside the rotor during centrifugation The tube cavities are numbered to aid in sample identification The rotor is specially designed with a fluid containment annulus located below the O ring sealing surface The annulus retains fluid that may escape from leaking or overfilled tubes thereby preventing the liquid from escaping into the instrument chamber The ultracentrifuge identifies rotor speed during the run by means of a magnetic speed sensor in the instrument chamber and magnets on the bottom of the rotor This overspeed protection system ensures that the rotor does not exceed its maximum permitted speed See the Warranty at the back of this manual for warranty information PREPARATION AND USE Specific information about the MLA 80 rotor is given here Informa tion common to this and other rotors is contained in Rotors and Tubes for Tabletop Preparative Ultracentrifuges publication TLR IM which should be used together with this manual for complete rotor and accessory operation Publication TLR IM is included in the literature package shipped with the rotor PRERUN SAFETY CHECKS A ROTOR PREPARATION MLA 80 Rotor gt NOTE Although rotor components and accessories made by other manufacturers may fit in the MLA 80 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the MLA 80 rotor may void
6. Series Ultracentrifuge EE 5 years No Proration Airfuge Ultracentrifuge Rotors 1 year No Proration For Zonal Continuous Flow Component Test and Rock Core ultracentrifuge rotors see separate warranty Warranty Conditions as applicable 1 This warranty is valid for the time periods indicated above from the date of shipment to the original Buyer by Beckman Coulter or an authorized Beckman Coulter representative 2 This warranty extends only to the original Buyer and may not be assigned or extended to a third person without written consent of Beckman Coulter 3 This warranty covers the Beckman Coulter Centrifuge Systems only including but not limited to the centrifuge rotor and accessories and Beckman Coulter shall not be liable for damage to or loss of the user s sample non Beckman Coulter tubes adapters or other rotor contents 4 This warranty is void if the Beckman Coulter Centrifuge Sys tem is determined by Beckman Coulter to have been operated or maintained in a manner contrary to the instructions in the operator s manual s for the Beckman Coulter Centrifuge System components in use This includes but is not limited to operator misuse abuse or negligence regarding indicated main tenance procedures centrifuge and rotor classification require ments proper speed reduction for the high density of certain fluids tubes and tube caps speed reduction for precipitating gradient materials and sp
7. 12 homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation The dashed lines represent equation 6 and are shown here to illustrate the inability of that equation to predict CsCl precipitation 1 90 1 80 m 1 70 z0 000 m f 40 000 p i I 5 1 60 RES w 9 Sal GOIN coe pa _ 1 50 Ba ASE Z z Beck 5 ES E A m KEE gt o A E7 A S C ot Q 1 40 amp 8 o gt ef A 1 30 RO gt lt gt f ee ER D Hy 1 20 j MLA 80 ROTOR 1 10 20 C 4 C R 1 00 300 330 38 0 43 0 48 0 53 0 58 0 Distance from Axis of Rotation mm Figure 3 CsCl Gradients at Equilibrium for the MLA 80 Rotor Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds from Figure 2 results in gradients presented here MLA SO Rotor
8. 55 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100022 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmBH Siemensstrasse 1 D 85716 Unterschleissheim Lohhof Germany Telephone 89 35870 0 Fax 89 35870 490 e mail bioresearch de beckman com Berg COULTER vu Beckman Coulter Inc e HONG KONG Beckman Coulter Hong Kong Ltd 15th Floor Gee Chang Hong Centre 65 Wong Chuk Hang Road Aberdeen Hong Kong Telephone 852 2814 7431 2814 0481 Fax 852 2814 1599 2873 4511 ITALY Beckman Coulter S p a Centro Direzionale Lambardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 e mail beckman_italia beckman com JAPAN Beckman Coulter K K Toranomon 37 Mori Bldg 3 5 1 Toranomo
9. BILITY AND BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFAC TURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Factory Rotor Inspection Service Beckman Coulter Inc will provide free mechanical and metallurgical inspection in Palo Alto California USA of any Beckman Coulter rotor at the request of the user Shipping charges to Beckman Coulter are the responsibility of the user Rotors will be inspected in the user s laboratory if the centrifuge in which they are used is covered by an appropriate Beckman Coulter Service Agreement Contact your local Beckman Coulter office for details of service coverage or cost Before shipping contact the nearest Beckman Coulter Sales and Service office and request a Returned Goods Authorization RGA form and packaging instructions Please include the complete rotor assembly with buckets lid handle tube cavity caps etc A SIGNED STATEMENT THAT THE ROTOR AND ACCESSO RIES ARE NON RADIOACTIVE NON PATHOGENIC NON TOXIC AND OTHERWISE SAFE TO SHIP AND HANDLE IS REQUIRED Beckman Coulter Worldwide Bioresearch Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd 24 College Street Unit D Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email myork beckman com CANADA Beckman Coulter Canada Inc 67
10. TL TB 024A October 1999 BECKMAN COULTER MLA 80 ROTOR Used In Beckman Coulter Optima MAX and MAX E Ultracentrifuges 1999 Beckman Coulter Inc PUBLISHED BY THE SPINCO BUSINESS CENTER OF BECKMAN COULTER INC PALO ALTO CALIFORNIA 94304 MLA S80 Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter ultracentrifuge that has been modified without Beckman Coulter s approval A Es 2 Ss ES e Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and
11. ble polypropylene rotor vise 346133 Place the lid on the rotor and tighten it firmly to the right clockwise by hand No tool is required Use an absorbent towel to wipe off condensation from the rotor Carefully place the rotor on the drive hub Refer to the instrument instruction manual for ultracentrifuge operation For additional operating information see the following e RUN TIMES page 9 for using k factors to adjust run durations e RUN SPEEDS page 10 for information about speed limitations e SELECTING CsCl GRADIENTS page 14 for methods to avoid CsCl precipitation during centrifugation MLA SO Rotor REMOVAL AND SAMPLE RECOVERY TUBES AND ACCESSORIES 25 C Com gt A CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Remove the rotor from the ultracentrifuge and place it in the rotor vise 2 Remove the lid by unscrewing it to the left counterclockwise 3 Use a tube removal tool to remove the spacers and tubes The MLA 80 rotor uses tubes and accessories listed in Table 1 Be sure to use only those items listed and to observe the maximum speed limits shown Refer to Appendix A in Rotors and Tubes for information on the chemical resistances of tube and accessory materials Temperature Limits e Plastic tubes have been centrifuge tested fo
12. certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the ultracentrifuge Although rotor components and accessories made by other manufacturers may fit in the MLA 80 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the MLA 80 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density Make sure that cavities in use have the proper spacers a
13. closed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office MLA SO Rotor SUPPLY LIST IR NOTE To obtain copies of referenced publications contact Beckman Coulter Inc Technical Publi cations Department 1050 Page Mill Road Palo Alto CA 94304 U S A telephone 650 859 1753 fax 650 859 1375 See the Beckman Coulter Ultracentrifuge Rotors Tubes amp Accesso ries catalog BR 8101 or contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS MLA 80 rotor assembly 367087 Ld pine ee ea os ee Rhee ce Bt aA ele 367089 Dn Re Et 892292 ROTOR VISE Les A RL Ee dr Aer tit 346133 OTHER Tubes and accessories
14. ed to approximate the banding positions of sample particles Gradient curves not shown in Figure 3 can be interpolated TYPICAL EXAMPLES FOR DETERMINING CsCl RUN PARAMETERS 14 Example A A separation that is done frequently is the banding of plasmic DNA in cesium chloride with ethidium bromide The starting density of the CsCl solution is 1 55 g mL In this separation the covalently closed circular plasmid bands at a density of 1 57 g mL while the nicked and linear species band at 1 53 g mL At 20 C where will particles band At Speed Floating Components Bands Pelleted Material At Rest in Rotor Upright MLA SO Rotor 1 In Figure 2 find the curve that corresponds to the desired run temperature 20 C and fill volume full The maximum allow able rotor speed is determined from the point where this curve intersects the homogeneous CsCl density 63 000 rpm 2 In Figure 3 sketch in a horizontal line corresponding to each particle s buoyant density 3 Mark the point in the figure where each particle density intersects the curve corresponding to the selected run speed and temperature 4 Particles will band at these locations across the tube diameter at equilibrium during centrifugation In this example particles will band about 44 0 and 46 5 mm from the axis of rotation about 2 5 mm of centerband to centerband separa tion at the rotor s 26 degree tube angle When the tube is held upright there wi
15. eed reduction for high temperature operation 5 Rotor bucket sets purchased concurrently with or subsequent to the purchase of a Swinging Bucket Rotor are warranted only for a term co extensive with that of the rotor for which the bucket sets are purchased 6 This warranty does not cover the failure of a Beckman Coulter rotor in a centrifuge not of Beckman Coulter manufacture or if the rotor is used in a Beckman Coulter centrifuge that has been modified without the written permission of Beckman Coulter or is used with carriers buckets belts or other devices not of Beckman Coulter manufacture 7 Rotor parts subject to wear including but not limited to rotor O rings VTi NVT TLV MLN and TLN rotor tube cavity plugs and gaskets tubing tools optical overspeed disks bear ings seals and lubrication are excluded from this warranty and should be frequently inspected and replaced if they become worn or damaged 8 Keeping a rotor log is not mandatory but may be desirable for maintenance of good laboratory practices Repair and Replacement Policies 1 Ifa Beckman Coulter rotor is determined by Beckman Coulter to be defective Beckman Coulter will repair or replace it subject to the Warranty Conditions A replacement rotor will be warranted for the time remaining on the original rotor s warranty 2 If a Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty Beckman Coulter will suppl
16. f the neck Do not leave a large air space too much air can cause excessive tube deformation e Refer to Rotors and Tubes for detailed information on the use and care of Quick Seal tubes Some of the tubes listed in Table 1 are part of the g Max system The g Max system uses a combination of small bell top Quick Seal tubes and floating spacers also called g Max spacers This means that you can run the shorter tubes listed in the table in the MLA 80 rotor without reduction in g force Additional information about the g Max system is available in publication DS 709 MLA SO Rotor Thickwall Tubes Thickwall polyallomer and polycarbonate tubes can be run partially filled at least half filled without caps but all opposing tubes for a run must be filled to the same level with liquid of the same density Do not overfill capless tubes RUN TIMES TIME HR MIN The k factor of the rotor is a measure of the rotor s pelleting x S efficiency Beckman Coulter has calculated the k factors for all of its L If j preparative rotors at maximum rated speed and using full tubes The k factor is calculated from the formula Si In max min 101 Re 1 oi 3600 where is the angular velocity of the rotor in radians per second 0 105 x rpm Fmax is the maximum radius and Fmin is the minimum radius After substitution 258 10 In max Fin e rpm Use the k factor in the following equation to estimate the run
17. he added tube load Note however that the use of this formula may still produce maximum speed values that are higher than the limitations imposed by the use of certain tubes or adapters In such cases use the lower of the two values 2 Further speed limits must be imposed when CsCl or other self forming gradient salts are centrifuged as equation 6 does not predict concentration limits speeds that are required to avoid precipitation of salt crystals Precipitation during centrifugation would alter the density distribution of CsCl and this would change the position of the sample bands Figures 2 and 3 together with the description and examples below show how to reduce run speeds when using CsCl gradients Table 2 Relative Centrifugal Fields for the MLA 80 Rotor Entries in this table are calculated from the formula RCF 1 12r RPM 1000 and then rounded to three significant digits Relative Centrifugal Field x g Rotor Speed At Fmax At fay At fmin k rpm 61 9 mm 45 7 mm 29 5 mm Factor 80 000 444 000 328 000 212 000 29 75 000 390 000 288 000 186 000 33 70 000 340 000 251 000 162 000 38 65 000 293 000 216 000 140 000 44 60 000 250 000 184 000 119 000 52 55 000 210 000 155 000 100 000 62 50 000 173 000 128 000 66 900 75 45 000 141 000 104 000 52 900 93 40 000 111 000 81 900 117 35 000 84 900 62 700 40 500 153 30 000 62 400 46 100 29 700 209 25 000 43 300 32 000 20 700 300 20 000 27 700 20 500 13 200 469
18. ll be about 2 8 mm of centerband to centerband separation This interband distance dup can be calculated from the formula de dup cos where dg is the interband distance when the tube is held at an angle 6 in the rotor Example B Knowing particle buoyant densities such as 1 59 and 1 54 g mL how do you achieve good separation 1 In Figure 3 sketch in a horizontal line corresponding to each particle s buoyant density 2 Select the curve at the desired temperature 4 C that gives the best particle separation 3 Note the run speed along the selected curve 50 000 rpm 4 From Figure 2 select the maximum homogeneous CsCl density in this case 1 60 g mL that corresponds to the temperature and run speed established above These parameters will provide the particle banding pattern selected in Step 2 In this example particles will band at about 45 0 and 40 0 mm from the axis of rotation about 5 0 mm apart When the tube is held upright there will be about 5 6 mm of center of band to center of band separation 15 MLA S80 Rotor CARE AND MAINTENANCE MAINTENANCE Lid 367089 E Lid O ring 892292 SS Check for Corrosion CLEANING Rotor Cleaning Kit 339558 16 IR NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Regularly
19. lubricate the metal threads in the rotor with a thin even coat of Spinkote lubricant 306812 Failure to keep these threads lubricated can result in damaged threads Regularly apply silicone vacuum grease to the O ring Replace the O ring about twice a year or whenever worn or damaged Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials Your Beckman Coulter representa tive provides contact with the Field Rotor Inspection Program and the rotor repair center Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues 1 2 Remove the O ring before washing Wash the rotor and lid in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water I gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight Lid O ring 892292 DECONTAMINATION MLA SO Rotor 3 Rinse the cleaned rotor and components with distilled water 4 Air dry the rotor and lid upside down Do not use acetone
20. n Minato ku Tokyo 105 0001 Japan Telephone 03 5404 8395 Fax 03 3578 4606 MEXICO Beckman Coulter de Mexico Calle Adolfo Prieto 1213 Colonia Del Valle Mexico D F C P Mexico 03100 Telephone 525 559 16 35 Fax 525 559 36 12 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 331 North Bridge Road Odeon Towers 07 01 02 Singapore 188720 Telephone 65 339 3633 Fax 65 336 6303 SOUTH AFRICA Beckman Coulter Stand 1A Fedsure Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 SPAIN Beckman Coulter Espa a S A Avda del Llano Castellano 15 28034 Madrid Spain Telephone 91 728 7900 Fax 91 728 7971 SWEDEN Beckman Coulter AB Archimedesvaegen 2 Box 11156 168 11 Bromma Sweden Telephone 08 98 53 20 Telefax 08 98 74 34 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court King
21. nd or floating spacers inserted before installing the rotor lid If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS and derate the run speed as appropriate Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use MLA SO Rotor MLA 80 ROTOR Tmax Axis of Rotation SPECIFICATIONS Maximum speed 80 000 rpm Density rating at maximum gpeed n usuuus narenn 1 2 g mL Relative Centrifugal Field at maximum speed At Sos OLO MM NEEN 444 000 x g At Fay ASTM EE 328 000 x g AT Pin 2905 MM 25803 Et Beh Ba ant 212 000 x g k factor at maximum speed 29 Conditions requiring speed reductions see RUN SPEEDS Number of tube cavities 8 Available tubes 1880828 SA Bie eS see Table 1 Nominal tube dimensions largest tube 16 x 64 mm Nominal tube capacity largest tube 8 mL Nominal rotor capacity 64 mL Approximate acceleration time to maximum speed fully loaded
22. r use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation MLA 80 Rotor Table 1 Available Tubes for the MLA 80 Rotor Use only the items listed here and observe maximum fill volumes and speed shown Tube Required Accessory Max Speed Dimensions Part Part DCH and Volume Description Number Description Number k Factor 16 x 58 mm Quick Seal 344621 Noryl floating 367094 80 000 rpm 8mL polyallomer pkg 50 spacer 444 000 x g 29 16 x 64 mm thickwall 355646 none 35 000 rom 6 5 mL polyallomer pkg 25 84 900 x g 153 16 x 64 mm thickwall 355647 none 80 000 rpm 6 5 mL polycarbonate pkg 25 418 000 x g 19 16 x 45 mm Quick Seal 345830 Noryl floating 367094 80 000 rom 6 3 mL polyallomer pkg 50 spacer 444 000 x g 23 16 x 38 mm Quick Seal 356562 Noryl floating 367094 80 000 rpm 4 2 mL polyallomer pkg 50 spacer 444 000 x g 18 e Noryl is a registered trademark of General Electric g Max Spacer N L J Bell top Tube Quick Seal Tubes Quick Seal tubes must be sealed prior to centrifugation These tubes are heat sealed and do not need caps however spacers are required on top of the tubes when they are loaded into the rotor e Fill Quick Seal tubes leaving a small bubble of air at the base o
23. smead Business Park London Road High Wycombe Bucks HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman_uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 e Fullerton California 92834 3100 Sales 1 800 742 2345 e Service 1 800 551 1150 e Internet www beckmancoulter com Telex 678413 e Fax 1 800 643 4366 gt Go Printed on recycled paper 1999 Beckman Coulter Inc amp LS EN ISO 9001
24. the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Inspect the O ring and lid for damage the high forces generated in this rotor can cause damaged components to fail Make sure to use only tubes and accessories listed in Table 1 Check the chemical compatibilities of all materials used refer to Appendix A in Rotors and Tubes For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Lightly but evenly lubricate metal threads with Spinkote lubricant 306812 Apply a thin film of silicone vacuum grease 335148 to the O ring in the rotor lid Load the filled and sealed if applicable tubes symmetrically into the rotor see page 7 for tube information If fewer than eight tubes are being run they must be arranged symmetrically in the rotor see Figure 1 Opposing tubes must be filled to the same level with liquid of the same density MLA S80 Rotor OPERATION Figure 1 Arranging Tubes in the Rotor Two four six or eight tubes can be centrifuged per run if they are arranged in the rotor as shown Use the required spacers if necessary see Table 1 to complete the loading operation After the rotor is loaded insert it into the porta
25. time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 80 000 4 kone ki ES adj E run speed MLA S80 Rotor 10 RUN SPEEDS SPEED RPM RCF Run times can also be estimated from data established in prior exper iments if the k factor of the previous rotor is known For any two rotors a and b ba ka d 5 th ky For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rota tional speed is adjusted so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 2 Speeds must be reduced under the following circumstances 1 If nonprecipitating solutions more dense than 1 2 g mL are centrifuged the maximum allowable run speed must be reduced according to the following equation reduced maximum speed 80 000 rpm 1 2 g mL Cem 6 where p is the density of the tube contents This speed reduction will protect the rotor from excessive stresses due to t
26. to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to the lid O ring before replacing it in the groove in the lid Clean metal threads as necessary at least every 6 months Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O ring and wipe clean as necessary Clean the O ring groove with a cotton tipped swab Reapply a light film of silicone vacuum grease If the rotor or other components are contaminated with radioactive toxic or pathogenic materials follow appropriate decontamination procedures as outlined by appropriate laboratory safety guidelines and or other regulations Consult Appendix A in Rotors and Tubes to select an agent that will not damage the rotor STERILIZATION AND DISINFECTION Coo D 121 C e The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid and O ring from the rotor and place the rotor and lid in the autoclave upside down e Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterili
27. ty or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Flammability hazard Do not use in or near operating ultracentrifuges 17 MLA S80 Rotor 18 STORAGE RETURNING A ROTOR Ed When it is not in use store the rotor in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be en
28. y free of charge i all centrifuge parts required for repair except the drive unit which will be replaced at the then current price less a credit determined by the total number of revolutions or years completed provided that such a unit was manufactured or rebuilt by Beckman Coulter and ii if the centrifuge is cur rently covered by a Beckman Coulter warranty or Full Service Agreement all labor necessary for repair of the centrifuge 3 If a Beckman Coulter rotor covered by this warranty is dam aged due to a malfunction of a Beckman Coulter ultracentrifuge covered by an Ultracentrifuge System Service Agreement Beckman Coulter will repair or replace the rotor free of charge 4 If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube bottle tube cap spacer or adapter covered under the Conditions of this Warranty Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point 2 above and the replacement policy 5 Damage to a Beckman Coulter rotor or instrument due to the failure or malfunction of a non Beckman Coulter tube bottle tube cap spacer or adapter is not covered under this warranty although Beckman Coulter will assist in seeking compensation under the manufacturer s warranty Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTA
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