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Accela Open Autosampler - Getting Started Guide

1. Peripheral Control Stat Ready Start n Out Out mMm M pooo000 j Interconnect cable TSQ Series mass spectrometer right panel Adapter q Pad cable a N 30V Max yli l Start In 10 Accela Open Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Connecting the Accela Pump Adapter Cable Use the Accela Open Autosampler interconnect cable see Figure 2 on page 5 and the Accela Pump adapter cable see Figure 8 to interconnect the system modules for the following hardware configurations e Accela Open Autosampler Accela Pump and MSQ Plus Mass Detector e Accela Open Autosampler Accela Pump and an LTQ Series LCQ Fleet LXQ LTQ XL or LTQ Velos mass spectrometer Figure 8 Accela Pump adapter cable lt 23 cm 9 in gt TO 60157 63024 Em 60157 63022 mmm TO PUMP To connect the Accela Pump adapter cable 1 If you have not already done so plug the DB15 connector of the Accela Open Autosampler interconnect cable into the Interface 1 port of the Accela Open Autosampler 2 Connect the 25 pin connector of the Accela Pump adapter cable to the 25 pin connector of the Accela Open Autosampler interconnect cable see Figure 9 Figure 9 Accela Open Au
2. Accela Open Autosampler Accela 600 or 1250 Pump INTERFACE 1 back panel MSQ Plus or LTQ Series _ Mass Detector mass spectrometer User I O ay Start n Peripheral Control q Start In st tat Ready Start e hn O Out me Ef erous E S Accela 600 or i Ready Out Y 2 1250 Pump A D SUB25 0 e T ge aa Accela Open Autosampler S interconnect cable G 6 Accela Open Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector Use the Accela detector and MS detector adapter cable see Figure 4 to connect the Accela Open Autosampler to an Accela detector a TSQ or Exactive mass spectrometer or both Figure 4 Adapter cable for an Accela detector and a TSQ Series MS detector P N 60157 63026 23 cm 9in gt E9 Si 60157 63024 Rw 60157 63026 Ran LCO FLEET TO LTQ LXQ MSQ 2 pin male 8 pin female connector 2 pin female connectors connector TO TSO EXACTIVE 200 cm 79 in gt To connect the LC detector and MS detector adapter cable 1 If you have not already done so connect the Accela Open Autosampler interconnect cable to the autosampler and the Accela 600 or 1250 Pump see Connecting the Accela Open Autosampler Interconnect Cable on page 5 2 Connect the adapter cable to the interconnect cable by
3. Parameter Description Method Use this list to select a method file The visible DLW methods in the Method dropdown list are only available if you have installed the DLW option that comes with your automsampler Description This box contains a description of the selected method Syringe This box displays the configured syringe Device Status This box displays the status of the PAL Status states Vial Index 1 In this box type the vial index to be used with the method SL index 2 In this box type the vial index to be used with the method SL index2 Trays 1 From this list select the tray to be used with the method SL tray 2 From this list select the tray to be used with the method SL tray2 Volume 1 In this box type the injection volume to be used with the method SL volume 2 In this box type the second injection volume to be used with the method SL volume2 Injectors 1 From this list select the injector to be used with the method SL injector 2 From this list select the second injector to be used with the method SL injector2 Injections Per Vial Type the number of replicate injections to be made from each vial Run Runs the selected method Accela Open Autosampler User Guide 57 4 Using the Direct Controls to Operate the Autosampler Changing the Tray Configuration Changing the Tray Configuration Use the Tray Configuration dialog box to specify the tray types to be used
4. 59 Wrapping Direct Control Injections with Additional Macros 62 Using Dynamic Load and Wash DLW 0000cceeeeeeeeees 63 COM EEVIGW farce Gade geile Ends EE wali gaits tn a ot chp a cts A EE E A 63 Installing Cycle Composer Macros or ICC Cycles 00 0 cece eee 64 General Considerations facies case a pe whet e ee bi caste ga 66 Priming the Solvent Lines Wash1 and Wash2 00000000 66 Standard DLW Injection Cycl se tack eh he Ma ee BN eo 68 Fast DLW Injection Cyclonic co innere enhi i ina e aera wea 70 Operating Dynamic Load and Wash DLW 0 0 cece eee eee 72 Priming che Solent hates s f 4 Met haere eh Palen e We aa ay ds 72 Location of Solvent and Waste Bottles n n naunnuuu eee eee 73 DLW PUM pS ons etp ern enr ge a a e So pie eos OEE ERE 73 DLW Actuator Solenoid is bcs the Sei ae dal wi ewe eS 73 DEW Cycle Step by Step ocna Sat wary E rd ean weet oe oats 75 Cycle for Standard Injection t tiesiclo ene pile eal es hae 75 Additional Valve Toggle Step to DLW Standard Cycle 4 88 Cycle for Fast Injection oe vai iaies See tian ese aude Re et eal 97 ES E E EE E E E E nee wan 109 viii Accela Open Autosampler User Guide Thermo Scientific Oo Y Preface Accela Open Autosampler manufactured by CTC Analytics is supplied by Thermo Fisher Scientific as part of a solution for high throughput LC MS applications Thermo Pal one of the device drivers provi
5. 2011 Thermo Fisher Scientific Inc All rights reserved The following are registered trademarks in the United States and possibly other countries Agilent and ChemStation are registered trademarks of Agilent Technologies Inc Analyst is a registered trademark of AB Sciex Pte Ltd Cheminert is a registered trademark of Valco Instruments Company Inc Kalrez is a registered trademark of E I du Pont de Nemours amp Company PAL is a trademark of CTC Analytics AG Ryton is a registered trademark of Chevron Phillips Chemical Company Simriz is a registered trademark of Carl Freudenberg KG Corporation Windows is a registered trademark of Microsoft Corporation Empower and MassLynx are trademarks of Waters Corporation PEEK is a trademark of Victrex plc All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc
6. Accela Open Autosampler User Guide 67 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Standard DLW Injection Cycle Table 15 lists the Stand Injection Accela Open macro parameters Table 15 Stand Injection Accela Open macro parameters Sheet 1 of 3 Macro description The PAL system waits first for the Sync Signal Ready before the injection cycle is started Macro variable Remark Sync Signal setting Start The injection valve is brought in a Inject to defined Standby position Standby The Rear air segment is pulled into Airgap Volume the Holding Loop Filling Speed The sum of Rear Sample List and Front Volume is aspirated into the Front Volume Rear Volume Holding Loop SL volume The Front air segment is aspirated Airgap Volume Filling Speed Pullup Delay The injection unit moves to the DLW Wash Station Wash position The needle is inserted dipped for 1 second to wash the outer needle surface No plunger movement at this step The injection unit moves to the Inject to specified injection valve Front Volume The Front and Airgap Volume is Airgap Volume ejected Injection Speed The PAL system waits for the data Wait for DS system The injection valve is switched to Inject to Active position The time Pre Inject Delay is awaited Pre Inject Delay The loop is filled with the sample volume as specified in the sample List
7. e Ask The autosampler asks you if you want to overwrite existing macros e Always Imported macros overwrite existing macros c Click Import to import the macros 9 In the Active Trays area select the trays that you want to use You can use trays that have a Yes in the Allow Use column Clicking the tray name clears the Allow Use cell and removes the tray from the list of available trays in the Xcalibur data system IMPORTANT Use the autosampler control terminal to specify the tray information You can change the tray information when you are controlling the autosampler from the Xcalibur data system but these changes are temporary When you close and reopen the Xcalibur data system the tray information resets to the autosampler control terminal settings 10 To accept the configuration settings and close the Pal Configuration dialog box click OK 11 Configure the other instruments of your LC MS system such as the LC pump and the mass spectrometer 12 In the Thermo Foundation Instrument Configuration window click Done PAL Configuration Parameters Use the Pal Configuration dialog box to select the PC Com port where the autosampler is connected specify macros to import and select sample trays or stacks Table 4 Pal Configuration dialog box parameters Sheet 1 of 2 Parameter Description Communications Com Port Select the computer port where you plug the autosampler communication cable 18 Accela Open Autosam
8. Accela Open Autosampler User Guide SL volume Injection Speed Thermo Scientific Thermo Scientific 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Table 15 Stand Injection Accela Open macro parameters Sheet 2 of 3 Macro description The injection valve is switched to Standby position the loop content is injected Timer 1 Delay Stator Wash is started and a Start signal to the HPLC system is sent Macro variable Post Inject Delay Timer 1 The plunger of the DLW Syringe is pushed down to dispense the Rear Sample and Air Segment to Waste The Holding Loop is still filled with Wash Solvent 1 Syr Eject Speed The DLW Actuator Solenoid is activated to deliver Wash Solvent 2 into the Holding Loop to clean the injection valve from Port 1 to Port 2 For this step the needle tip is lifted releasing the sealing pressure to enable rinsing around the tip sealing point Wash2 Inject to Needle Gap Valve Clean Valve Clean Time Solvent 2 Remark For a detailed explanation of the Needle Gap Valve Clean see DLW Priming The injection unit is moved to the DLW Wash Station Wash2 position The needle is rinsed inside and out with Wash Solvent 2 Wash2 Syr Eject Speed Post Clean Time Solvent2 The injection unit is moved back to the injection valve The Inlet Port and engraving to waste Port are flushed with Wash Solvent 1 to prepare the valve f
9. Holding Loop SL volume Airgap Volume The Front air segment is aspirated Filling Speed Pullup Delay The injection unit moves to the specified Inject to injection valve The Front and Airgap Volume is ejected to Waste Front Volume Airgap Volume Injection Speed The PAL system waits for the data system Wait for DS The injection valve is switched to Active position The Pre Inject Delay time is awaited The loop is filled with the sample volume as specified in the sample list The injection valve is switched to Standb y position the loop content is injected Inject to Pre Injection Delay SL volume Injection Speed Post Inject Delay The plunger of the DLW Syringe is pushed down to dispense the Rear Sample and Air Segment to Waste The Holding Loop is still filled with Wash Solvent 1 Injection Speed The DLW Actuator Solenoid is activated to deliver Wash Solvent 2 into the Holding Loop to clean the injection valve from Port 1 to Port 2 For this step the needle tip is lifted releasing the sealing pressure to enable rinsing around the tip sealing point Inject to Wash2 Needle Gap Valve Clean Valve Clean Time Solvent 2 Remark For complete details on the Needle Gap Valve Clean see DLW Priming Accela Open Autosampler User Guide 71 5 Using Dynamic Load and Wash DLW Operating Dynamic Load and Wash DLW Table 16 Macro Fast Injection Accela Open Sheet 2 of 2
10. 78 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 41 Standard Steps 5 6 Passive needle clean outside dip in wash position 1 Solenoid Actuator Valve Column Thermo Scientific Accela Open Autosampler User Guide 79 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 42 Standard Steps 7 8 Dispense front air segment and front sample volume to waste Solenoid Actuator Valve 80 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 43 Standard Steps 9 10 Valve is switched to LOAD position loop is filled with Inject Volume Solenoid Actuator Valve Pump 1 lt gt gt Column Thermo Scientific Accela Open Autosampler User Guide 81 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 44 Standard Step 11 Valve is switched to INJECT position start chromatographic process Solenoid Actuator Valve Column 82 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 45 Standard Step 12 Rear sample volume and air segment are dispensed to waste Solenoid p iji Actuator Valve E Column E g E Thermo Scientific Accela Open Autosampler User Guide 83 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figu
11. Creating an Instrument Method This section provides information about how to create an instrument method To specify the instrument method parameters for the autosampler 1 Open the Xcalibur data system from the computer desktop as follows For Xcalibur version 2 1 0 or later choose Start gt All Programs gt Thermo Xcalibur gt Xcalibur The Roadmap Home Page appears claimed 2 Click 2 Instrument Setup The Instrument Setup window appears with the configured devices displayed in the view bar see Figure 16 By default the view for the first device is open as indicated by a bright green triangle in the lower right corner of the device 24 Accela Open Autosampler User Guide Thermo Scientific 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Figure 16 Instrument Setup window ES Untitled Thermo Xcalibur Instrument Setup BAR File AccelaOpenAS Help osm S x pA R R A A DA CO A DA DA CCL CO CCC A D OL R A A D R A D A A 2 Method Setup Method Summary Template Selection CENELEC emate l Bright green triangle dr Template Description r Mac Var 2 Thermo Pal 2 A a p Syringe Dooa A z A m Macro Sequence A N E 3 In the view bar click Accela Open AS The Method Setup page for the autosampler appears see Figure 17 on page 26 Thermo Scientific Accela Open Autosampler User Guide 25 2 Getting Started Specifying the In
12. Pump 94 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 56 Stator Wash Step 5 Second valve is cleaned with Wash Solvent 1 Solenoid Actuator Valve Column Pump Thermo Scientific Accela Open Autosampler User Guide 95 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 57 Stator Wash Step 6 Valve switched back to Inject Position toggle Solenoid Actuator Valve Pump 1 lt gt Pump 2 gt Column Pump 96 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Cycle for Fast Injection Figure 58 to Figure 69 illustrate a step by step cycle for the Fast Injection Figure 58 Fast Cycle start Solenoid Actuator Valve Pumpi lt gt Pump2 c gt Holding Loop Thermo Scientific Accela Open Autosampler User Guide 97 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 59 Fast Step 1 Aspirate rear air segment Solenoid Actuator Valve Pump 1 gt Pump2 gt Holding Loop Column 98 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 60 Fast Step 2 Get sample aspirate rear inject and front volume Solenoid Actuator Valve Pump gt Pump2 c gt Holding Loop Thermo Scientific Accela
13. Syringe This list contains the allowed syringe volumes Select the appropriate syringe volume Macro Sequence Displays the sequence of macros that make up the method chosen in the Template window Select a macro name to be displayed in the Macro area its variables also appear in the Variables area Recommended Injection Volume Volume pl When you select an instrument method for use to generate a new sequence on the Xcalibur Home Page the injection volume is set to what is entered in this box e When From AS From Auto Sampler is specified in the LCquan injection volume sequence cell the CTC PAL autosampler uses the volume in this box when you submit the LCquan sequence for acquisition Look Ahead Injections Delay Time mins Enter the Delay Time in minutes between injections when using the Look Ahead feature You enable the Look Ahead Injections option from the Pal Configuration dialog box When you enable this option the next Look Ahead Injection is delayed the amount of time entered in this box The delay time countdown begins at the completion of any post injection steps This prevents a sample from being held in the injection syringe for the duration of the current run If the entered Delay Time is too long or the current run ends prematurely the entered Delay Time is canceled and the next injection commences in the normal manner Macro The Macro area shows the name of the macro selected in the
14. The DLW macro definitions Macro name Macro description Priming AccelaOpen For initial and daily routine priming of the solvent lines and DLW manifold The Clean Time for both wash solvents is a variable for the user to define the intensity of washing Standard Injection Standard injection cycle using all possibilities of the DLW option Accela Open The injection valve inlet port and the needle are washed with both wash solvents inside and out You can add an extra Stator Wash for intensive washing of the injection valve valve toggle Fast Injection Accela Tuned for speed and high throughput application It differs from Open the Standard DLW macro in that some steps are left out to shorten the cycle time Installing the Cycle Composer Macros or ICC Cycles Thermo Scientific The autosampler is shipped with a CD ROM containing various cycles for the DLW option Macros for the Xcalibur data system are installed by the LC Devices 2 4 installer Copy these macros to your Thermo PAL driver method folder or corresponding folder for application within the integrated system 1 bd P To copy cycles to your Cycle Composer Navigate in Windows Explorer to the Thermo PAL driver folder The usual folder location is as follows C Thermo Instruments LC Devices ThermoPAL PAL Ifyou wish to add the DLW option macros to an existing method folder copy the macros pma and the methods pme files from the DLW Optio
15. E LC Inj DLW Standard RevO3 3KB PMEFile 3 31 2010 9 28 AM E lt amp Local Disk C E Multi Step LC Injection PME 4KB PMEFile 3 31 2010 9 28 AM Program Files E Priming Accela Open pme 2KB PMEFile 3 31 2010 9 28 AM GS Projects E single Step LC Injection PME 2KB PMEFile 3 31 2010 9 28 AM Quarantine Z Standard Injection Accela Op 3KB PMEFile 3 31 2010 9 28 AM Tech Pubs E Startup Shutdown PME 1KB PMEFile 3 31 2010 9 28 AM B test Thermo amp O Instruments S LC Devices amp AccelaOpenas par Macros 5 In the Template list select a template that represents the task that you want to do The autosampler comes with these templates e DLW Priming Rev0l pme e Fast Injection Accela Open pme e LC Inj DLW Fast_Rev03 pme LC Inj DLW Standard_Rev03 pme e Multi Step LC Injection pme e Priming Accela Open pme e Single Step LC Injection pme e Standard Injection Accela Open pme Startup Shutdown pme There are three templates in instrument setup designed to be used for Accela Open Autosampler with Dynamic Load and Wash DLW They are as follows e Fast Injection Accela Open pme e Standard Injection Accela Open pme e Priming Accela Open pme WARNING Do not use the Single Step LC Injection pme and Multi Step LC Injection pme templates for Accela Open AS with DLW These are settings for Accela Open AS without DLW and Thermo PAL Using these templ
16. Inject to Delay Stator Wash Active Inject to Wash2 Stator Wash Time Solvent 2 Wash Solvent is changed to Wash Solvent 1 Inject to Wash 1 Stator Wash Time Solvent 1 The injection valve is switched back to the Standby position Fast DLW Injection Cycle 70 Inject to Standby The Fast injection cycle differs from the Standard cycle as follows e The needle is not dipped in the Wash station Wash1 after sample pickup and before it moves to the injection valve e The wash steps after injection are reduced to Valve Clean with Wash Solvent 1 and Wash Solvent 2 The DLW needle is flushed in the DLW Wash Station with Wash Solvent 1 only e Stator Wash valve toggle is not available Accela Open Autosampler User Guide Thermo Scientific Thermo Scientific 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Table 16 lists the Fast Injection Accela Open macro parameters Table 16 Macro Fast Injection Accela Open Sheet 1 of 2 Macro description The PAL system waits first for the Sync Signal Ready before the injection cycle is started Macro variable Remark Sync Signal setting Start The injection valve is brought to a defined Inject to position Standby Standby The Rear air segment is pulled into the Holding Airgap Volume Loop Filling Speed The sum of Rear Sample List and Front Volume is aspirated into the Front Volume Rear Volume
17. LC MS system includes an Accela detector or a TSQ Series or Exactive mass spectrometer go to Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector on page 7 e Or if your LC MS system includes an MSQ Plus Mass Detector or an LTQ Series mass spectrometer but does not include an Accela detector go to step 4 4 Connect the cable labeled TO MSQ LTQ LXQ to the mass spectrometer Do one of the following e For the MSQ Plus Mass Detector connect the cable to the User I O Start In pins on the MS detector s back panel see Figure 3 For the LTQ Series mass spectrometer connect the cable to the Peripheral Control Start In pins on the MS detector s right panel see Figure 3 Accela Open Autosampler User Guide 5 1 Getting Connected System Synchronization Connections Figure 3 Accela Open Autosampler interconnect cable connections back panel view of the LC MS modules Accela Open Autosampler back panel MSQ Plus Mass Detector LTO Series mass spectrometer back panel back panel
18. Macro description Macro variable Wash Solvent 1 follows to prepare the valve for Wash1 the next injection Inject to Needle Gap Valve Clean Valve Clean Time Solvent 1 The injection unit is moved back to the DIW Wash1 Wash station Wash1 position to flush the syringe Post Clean Time Solvent 1 needle inside and out with Wash Solvent 1 This is a preparation step for the next injection and is especially important for biofluid samples Cycle end for LC Inj DLW Fast macro Operating Dynamic Load and Wash DLW This section describes how to operate the Dynamic Load and Wash DLW option e Priming the Solvent Lines e Location of Solvent and Waste Bottles e DLW Pumps Priming the Solvent Lines Note For trouble free DLW operation make sure the two solvent lines are free of air bubbles at all times If the solvent lines are being connected for the first time or during a solvent change you must prime the solvent lines properly until air bubbles are no longer visible Use solvent degassing for best results To make the initial and daily priming efficient and controllable the Open Autosampler comes with a Cycle Composer macro or ICC cycle To prime the solvent lines 1 Load the macros and methods into the folder 2 Start the corresponding macro for initial or daily priming 3 Check the solvent lines and prime until air bubbles are no longer visible 4 Press F4 for Home 72 Accela Open Autosampler User Guide Ther
19. Menu by pressing the F1 key see Figure 14 Figure 14 Autosampler control terminal with the Job Queue screen displayed JOB QUEUE Menu Ins Del Pause Job Job F1 F2 F3 F4 ESG Q STOP 2 From the Menu screen open the Tray screen as follows Inner knob button Outer knob a Rotate the outer knob to move the cursor bar highlight to Setup b Press the inner knob button to select Setup c Rotate the outer knob to move the cursor bar to Objects d Press the inner knob button to select Objects e Rotate the outer knob to move the cursor bar to Trays f Press the inner knob button to select the Trays screen The Trays screen lists the available trays Accela Open Autosampler User Guide 21 2 Getting Started Using the Autosampler Control Terminal 3 Select a tray as follows a Rotate the outer knob to move the cursor bar to the tray to be selected b Select the tray by pushing the inner knob button The screen now shows the Tray Type c Make the Tray Type active by pushing the inner knob button The Tray Type is highlighted d Rotate the outer knob to select the tray type Select NONE if no tray is present rotate the outer knob until NONE appears as the Tray Type e Push the inner knob button to select this tray type 4 Repeat step 3 for all the trays to be used 5 Return to the Job Queue screen by push
20. Note Thermo Fisher Scientific has discontinued the Accela Pump To connect the required cables listed in Table 3 follow these procedures as appropriate e Connecting the Accela Open Autosampler Interconnect Cable e Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector e Connecting the Accela Pump Adapter Cable Accela Open Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Connecting the Accela Open Autosampler Interconnect Cable Figure 2 shows the Accela Open Autosampler interconnect cable P N 60157 63024 Figure 2 Accela Open Autosampler interconnect cable P N 60157 63024 lt 102 em 40 in gt TO MSQ LTO LXO 305 cm 120 in p To connect the Accela Open Autosampler interconnect cable to the LC or LC MS system modules 1 Plug the DB15 connector cable end labeled TO A S into the Interface 1 receptacle on the back panel of the Accela Open Autosampler see Figure 3 2 Depending on the Accela pump model do one of the following e Ifyour LC system includes an Accela 600 or 1250 Pump plug the 25 pin connector cable end labeled TO PUMP into the D Sub25 receptacle on the back panel of the pump seeFigure 3 e Ifyour LC system includes an Accela Pump go to Connecting the Accela Pump Adapter Cable on page 11 3 Depending on the LC detector or MS detector setup do one of the following e Ifyour LC or
21. Selection Template Description This box shows the names of the default method templates After you select a template the Template box changes to a pane This pane shows the path to the chosen method template file Method Folder This pane shows the path to the default template folder location before a default method template is chosen After a default method template is chosen in the Template box the Method Folder label disappears leaving the Template label next to a pane that shows the path to the chosen template Browse This button appears next to the Method Folder pane before the method template is chosen in the Template box Click the Browse to search the computer file system for the method template of your choice When you select a method template the window changes to show the path to the chosen template and the Browse button changes to the New Template button New Template This button appears after choosing a method template in the Template box Selecting this button brings back the Template box Method Folder pane and Browse button which are used to find a new method template Template Description A description of the template chosen in the Template Selection portion of the Method Setup page Accela Open Autosampler User Guide 31 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Table 5 Method Setup page parameters Sheet 2 of 3 Parameter Description
22. The pumps are self priming with a suction lift of up to 3 m water column DLW Actuator Solenoid Thermo Scientific The DLW Actuator Solenoid has the function of separating and completely shutting off the lines in the direction of the syringe sample loading or the wash solvent lines After opening the DLW Actuator Solenoid for the wash solvent lines you can pump the desired wash solvent into the system by activating the corresponding DLW pump Figure 66 on page 105 illustrates this functionality The wetted parts in the DLW Actuator Solenoid are made from the following materials e Solenoid body PEEK e Seal material FFKM Simriz Accela Open Autosampler User Guide 73 5 Using Dynamic Load and Wash DLW Operating Dynamic Load and Wash DLW Note PEEK exhibits excellent chemical resistance to most of the chemicals used However the following solvents are not recommended for use with PEEK DMSO THE methylene chloride dichloromethane nitric acid or sulfuric acid For more details refer to the compatibility tables provided by the manufacturer of PEEK material or components Note Current applied from the actuator control PCB to the actuator solenoid activates a pp green LED This activation does not indicate that the solenoid opens or closes Figure 35 DLW manifold and Actuator Solenoid Closed Solvent f DLW Flow i Diverter Solvent 1 _ Solvent 2 g 4 3 ii AM DL
23. box Run Step Automatically executes the macros that constitute the Method Macro Sequence Executes the Method Macro Sequence in a stepwise manner Macro Choose individual PAL macros in this list When you select a macro it is highlighted in blue Displayed at the top of the Macro list is the sequence number x of the chosen macro and the total number of macros y in the Macro Sequence pane Macro x of y You can place the chosen macro in the Macro Sequence box see explanation above by using the Insert Above Insert Below or Replace buttons as appropriate see corresponding explanations above Default All 38 Accela Open Autosampler User Guide Changes all of the Method Template Editor variables to their default values Thermo Scientific 3 Creating Custom Templates and Macros Testing a Custom Template Table 6 Template Editor page parameters Sheet 3 of 3 Parameter Description Variables The variables that make up the macro in the Macro list are displayed in this area Variables with specific allowed values discrete variables are displayed in lists Continuous variables are displayed in boxes You may choose the allowed values of discrete variables from those presented in the list for the variable The allowed values for continuous variables are displayed when you hold the cursor over the respective box for the variable Then choose the appropriate value for the continuous variable to type i
24. e Specifying the Location of the Direct Control Methods e Wrapping Direct Control Injections with Additional Macros Opening the Direct Control Dialog Box The direct control pages for the autosampler are available from the Instrument Setup window and the Tune window To open the Direct C ialog box from the Instrument Setup window 1 In the view bar click Accela Open AS The Instrument Setup view for the autosampler appears 2 From the menu bar choose Accela Open AS gt Direct Control The Direct Control dialog box appears with the Methods page displayed see Figure 30 on page 54 Thermo Scientific Accela Open Autosampler User Guide 53 4 Using the Direct Controls to Operate the Autosampler Opening the Direct Control Dialog Box Figure 30 Direct Control dialog box Direct Control Methods Tray Configuration Direct Control Configuration Method Vial Index Description r Syringe Device Status Ready To Download To open the Inlet Direct Control dialog box from the Tune window 1 From the menu bar choose Setup gt Inlet Direct Control 2 Click the Thermo Pal tab The Inlet Direct Control dialog box for the autosampler appears with the Methods page displayed The Inlet Direct Control dialog box has the same functionality as the Direct Control dialog box shown in Figure 30 54 Accela Open Autosampler User Guide Thermo Scientific 4 Usin
25. eee een eens 15 Adding the Autosampler to the Xcalibur Instrument Configuration 15 Firmware Versions Supported lt 5 6 0045 cee csraksacdeseeateew anne dace 20 Using the Autosampler Control Terminal 0 occ o0s ecw iene cceeeen nes 20 Selecting the Iray Typer sores insiden i e E E ee cee coes Leeman 21 Changing the Pulse Time Setting 20 0 0 0 cece ee eee eee ee 22 Specifying the Instrument Method Parameters for the Autosampler 24 Viewing the Method Summary 14 05 542450 8 deeacdisdeateciatecheutans 33 Chapter3 Creating Custom Templates and Macros 0020eeeeeee eens 35 Using the Template Editor to Create Custom Templates 35 Testing a Custom Template cc nips ies Mae cdd etoe ees eee ees MSs 39 Viewing the Template Suing cnt 44 oe ie head ko nes nner erena 42 Standard MICOS tte oie ig hee kere hee Shee EEEE 44 Using the Macro Editor to Create Custom Macros 0 0000000 ee 45 Defining VateDles ccc he keene einir oei a setae seas tees 50 Thermo Scientific Accela Open Autosampler User Guide vii Contents Chapter 4 Chapter 5 Using the Direct Controls to Operate the Autosampler 53 Opening the Direct Control Dialog Box 1 0 0 ee eee ee 53 Making Single Injections from the Tune Window 00008 55 Changing the Tray Configuration oi teien are oats a 8 are RON a alee da ees 58 Specifying the Location of the Direct Control Methods
26. for direct control injections while you have the Direct Control dialog box open The autosampler uses this tray information instead of the tray information specified in the autosampler Control Terminal IMPORTANT The autosampler does not store the tray configuration that you specify on the Tray Configuration page of the Direct Control dialog box When you close the dialog box the tray configuration resets to the autosampler Control Terminal settings To change the tray configuration 1 Open the Direct Control dialog box see Opening the Direct Control Dialog Box on page 53 2 Click the Tray Configuration tab The Tray Configuration page appears see Figure 32 Figure 32 Tray Configuration page Direct Control Methods Tray Configuration Direct Control Configuration Tray Tra Holder Type l Vials Inx Direction Vials In Y Direction Help 3 In the Tray list select the tray that you want to modify 4 In the Type list select the tray type 58 Accela Open Autosampler User Guide Thermo Scientific 4 Using the Direct Controls to Operate the Autosampler Specifying the Location of the Direct Control Methods Direct Control Tray Configuration Page Use the Tray Configuration page in the Direct Control dialog box to enter the Tray Configuration information required for the autosampler to carry out autosampling Table 11 Tray Configuration page parameters Parameter Description Tray From thi
27. makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history Revision A April 2010 Revision B March 2011 Software version Xcalibur 2 1 or later LC Devices 2 5 0 Firmware version 4 1 2 or later For Research Use Only Not for use in diagnostic procedures Regulatory Compliance Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations When the system is delivered to you it meets all pertinent electromagnetic compatibility EMC and safety standards Changes that you make to your system may void compliance with one or more of these EMC and safety standards Changes to your system include replacing a part or adding components options or peripherals not specifically authorized and qualified by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional co
28. plugging the 2 pin male connector labeled TO 60157 63024 on the adapter cable into the 2 pin female connector labeled TO MSQ LTQ LXQ on the interconnect cable see Figure 5 Figure 5 Accela Open Autosampler interconnect cable to detector adapter cable connection Accela Open Accela detector and Autosampler MS detector interconnect cable adapter cable Thermo Scientific Accela Open Autosampler User Guide 7 1 Getting Connected System Synchronization Connections 3 Using the adapter cable connectors make the appropriate connections to an Accela detector a Thermo Scientific mass spectrometer or both as follows e For an Accela detector UV Vis or PDA connect the 8 pin connector attached to the cable labeled TO PDA to pins 1 through 8 on the detector s back panel e For an MSQ Plus Mass Detector connect the 2 pin connector attached to the cable labeled TO LTQ LXQ MSQ to the Start In pins on the mass spectrometer s back panel e Foran LTQ Series mass spectrometer connect the 2 pin connector attached to the cables labeled TO LTQ LXQ MSQ and TO LCQ FLEET to the Start In pins on the right side of the mass spectrometer e Fora TSQ or Exactive mass spectrometer connect the cable labeled TSQ EXACTIVE to the Start In pins on the right side of the mass spectrometer Figure 6 and Figure 7 show the contact closure connections for a standalone Accela LC system and an LC MS system respectively 8 Accela O
29. that you want to inject e In the Injection Per Vial box type the number of injections that you want to make from each vial 5 Click Run The autosampler performs the actions specified in the template step by step At the completion of each step the autosampler pauses the injection sequence and prompts you with the Pal Method Editor dialog box see Figure 23 Figure 23 Pal Method Editor dialog box Pal Method Editor Current Macro is SL Entry Current Command is SET_OUT_SIG CCRuning 1 D Stop Run 40 Accela Open Autosampler User Guide Thermo Scientific 3 Creating Custom Templates and Macros Testing a Custom Template 6 Do one of the following e To continue stepping through the template click Step To run the remaining portion of the template click Run e To stop the run click Stop Run Torun the complete sequence of macros in the template without pausing 1 If the Template Editor dialog box is closed open it 2 If you closed the custom template choose File gt Template gt Open 3 Click Run The Run Auto Sampler Method dialog box appears see Figure 24 Figure 24 Run Auto Sampler Method dialog box Run Auto Sampler Method Vial Index Injectors 1 fl 1 ILC Viv 2 f 2 Lev Trays Volume 1 CStk1 01 z 1 fio 2 CStk1 01 x 2 fio Injections Per Vial Cancel Help 4 Make the appropriate entries and selections 5 Click Run The autosampler executes the macros listed in
30. the template Run Auto Sampler Method Dialog Box Use the Run Auto Sampler Method dialog box to enter the Methods information required for the PAL autosampler to carry out autosampling Thermo Scientific Accela Open Autosampler User Guide m 3 Creating Custom Templates and Macros Viewing the Template Summary Viewing the Template Summary 42 Table 7 Run Auto Sampler Method dialog box parameters Parameter Vial Index Description 1 In this box type the vial index to be used with the method SL index In this box type the vial index to be used with the method SL index2 From this list select the tray to be used with the method SL tray From this list select the tray to be used with the method SL tray2 Injectors 1 From this list select the injector to be used with the method SL injector From this list select the second injector to be used with the method SL injector2 Volume In this box type the injection volume to be used with the method SL volume In this box type the second injection volume to be used with the method SL volume 2 Injections Per Vial Run Type the number of replicate injections to be made from each vial Run the selected method Cancel Cancel any changes and close the page To view a summary of a template 1 Open the PAL Template Macro Editor window see Using the Template Editor to Create Custom Template
31. 11 12 Valve is cleaned with Wash Solvent 2 Solenoid Actuator Valve wu Thermo Scientific Accela Open Autosampler User Guide 105 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 67 Fast Steps 13 14 Valve is cleaned with Wash Solvent 1 Solenoid Actuator Valve Pump gt Pump 2 gt 106 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 68 Fast Step 15 Active syringe needle wash with Wash Solvent 1 Solenoid Actuator Valve Pump gt Pump2 gt Thermo Scientific Accela Open Autosampler User Guide 107 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 69 Fast Cycle end Solenoid Actuator Valve Pump lt gt Pump2 lt gt Holding Loop 108 Accela Open Autosampler User Guide Column Thermo Scientific Ooo Y Index A Accela Open Autosampler adapter cable for detector 7 interconnect cable 5 autosampler control terminal using 20 C cables Accela Open Autosampler system interconnect cable 5 Accela Pump adapter cable 11 DB9 to DB9 serial cable 14 detector adapter cable 7 compliance FCC iii regulatory iii WEEE v computer Com port 17 control terminal attached to the autosampler 20 cycle composer macros 64 cycle composer macros or ICC cycles installing 64 Cycle Editor 64 delay time 30 Direct Control Configuration page 59 D
32. Macro Sequence pane The sequence number x of the selected macro out of the total number of macros y in the Macro Sequence is also displayed next to the name of the selected macro Macro name x of y This area also lists the variables associated with the macro chosen in the Macro Sequence pane 32 Accela Open Autosampler User Guide Thermo Scientific 2 Getting Started Viewing the Method Summary Table 5 Method Setup page parameters Sheet 3 of 3 Parameter Variables Description The variable parameters used by the selected macro are displayed in this portion of the Macro area Variables with specific allowed values discrete variables are displayed in lists Continuous variables are displayed in panes You may choose the allowed values of the discrete variables from those presented in the list for the variable The allowed values for continuous variables are displayed when you hold the cursor over the respective pane for the variable You can type the value for the continuous variable in its pane Default All Viewing the Method Summary This button changes the variables used in the selected Macro Sequence to their default values To view a summary of the autosampler portion of an instrument method 1 Open the Instrument Setup view for the autosampler see Specifying the Instrument Method Parameters for the Autosampler on page 24 2 Choose File gt Open and select the instrument method that you want
33. Open Autosampler User Guide 99 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 61 Fast Step 3 4 Aspirate front air segment Solenoid Actuator Valve Pump gt Pump 2 gt Holding Loop 100 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 62 Fast Steps 5 6 Dispense front air segment and front sample volume to waste Solenoid Actuator Valve uuu Thermo Scientific Accela Open Autosampler User Guide 101 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 63 Fast Steps 7 8 Valve is switched to LOAD position loop is filled with Inject Volume Solenoid Actuator Valve Inject volume at Column Pump 102 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 64 Fast Step 9 Valve is switched to INJECT position start chromatographic process Solenoid Actuator Valve Pump Thermo Scientific Accela Open Autosampler User Guide 103 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 65 Fast Step 10 Rear sample volume and air segment are dispensed to waste Solenoid j Actuator Valve H Pump c gt wl 104 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 66 Fast Steps
34. Si Autosampler torir We ee back panel Boaaae J Fa a Te te Ea j j 5 _ k H m So om Accela Pump yl Te back panel ta L ltl Pi z aE aah p a awl y aa Q Q i 1 ay TO MSQ LTO LXO_ N LTO Series mass spectrometer right panel Interconnect cable MSQ Plus back panel cable gt I ss mii a e D S ae 4 g USER VORT seene u Bl Start In W i M a i o e POP ae o Ready Out aan x E BF Ready Out 7 veo a ae Dlo lt Thermo Scientific Accela Open Autosampler User Guide 13 1 Getting Connected Connecting the Accela Open Autosampler to the Data System Computer Connecting the Accela Open Autosampler to the Data System Computer To connect the Accela Open Autosampler to the data system computer Using the serial cable that is supplied with the autosampler connect the SER 1 port on the back panel of the autosampler to the serial port on the back panel of the data system computer see Figure 11 Figure 11 Data system connection firmware 4 1 2 Data system computer Autosampler o o o TERMINAL SERI LAN MODBUS suzen 4 wasi INTERFACE1 INTERFACE 2 AUX2 POWER FUSE mon CO e DB9 to DB9 serial cable 14 Accela Open Autosampler User Guide Thermo Scientific ee i G
35. T Template Editor enabling 17 opening 35 templates editing 35 Single Step LC Injection 27 testing 39 Thermo PAL driver for Xcalibur 64 Tray Configuration page 58 tray type selecting with the PAL Control Terminal 21 Trays screen 21 Tune window making single injections from 55 V variables defining 50 W Waiting for contact closure status for LC pump 22 110 Accela Open Autosampler User Guide WEEE compliance v Thermo Scientific
36. Thermo Scientific The DLW Standard Cycle has the built in option for the user to switch the injection valve at the end of the chromatographic run before equilibration of the column to the start conditions If the method variable Stator Wash is set to 1 the extra cleaning process for the valve with Valve Toggle is part of the standard cycle If the method variable is deactivated setting O the DLW Standard cycle ends as shown in Figure 50 The macro cycle is written so that the optional valve toggle steps can be executed before re equilibration of the column You must synchronize the time to switch the valve with the chromatographic method using the method variable Delay Stator Wash The two wash solvents are timed by the method variables Stator Wash Time Solvent 1 and Stator Wash Time Solvent 2 After these wash times have elapsed the valve is switched back to the start position Figure 51 illustrates the recommended retention time for Stator Wash or Valve Toggle times Figure 51 Timing for Stator Wash Step w Valve Toggle gt 2 O w ao 2 FENER ERETE ETENE Start Time From a chromatographic viewpoint the optional cleaning step is important to understand Assuming that the valve stator between ports 1 and 6 for example in the standard Cheminert valve is contaminated and cannot be cleaned during the injection process the valve toggle brings the engraving bac
37. Vinh Mi Add Edit Delete Command Sequence GET_SAMPLE Insert Above Insert Below Delete Save As Device Status Ready To Download 2 Under Macro select a macro from the list The description of the selected macro appears under Macro Description and a list of macro variables appears under Macro Variables 46 Accela Open Autosampler User Guide Thermo Scientific 3 Creating Custom Templates and Macros Using the Macro Editor to Create Custom Macros 3 To add a variable to the macro click Add The Variable Definition dialog box appears 4 Define the variable see Defining Variables on page 50 5 Save the macro as follows a Click Save As The Save As dialog box appears b Select a file location c In the File name box type an appropriate name d Click Save The autosampler saves the macro with the file extension pma PAL Template Macro Editor Page Use the Macro Editor page of the Pal Template Macro Editor window to create custom macros Table 8 Macro Editor page parameters Sheet 1 of 3 Parameter Description Macro This list displays existing autosampler macros Select the macro that you want to edit Macro Description A description of the macro selected in the Macro list Macro Variables Variables used by the macro selected in the Macro list Add Adds a new variable to the macro variables The new variable is defined in an Edit Parameter dialo
38. W Flow Holding Loop Diverter 74 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step DLW Cycle Step by Step This section provides illustrations to demonstrate a step by step DLW cycle e Cycle for Standard Injection e Additional Valve Toggle Step to DLW Standard Cycle e Cycle for Fast Injection Cycle for Standard Injection Figure 37 to Figure 50 illustrate a step by step cycle for the standard injection Figure 37 Standard Cycle start Solenoid Actuator Valve Pumpi gt Pump gt Holding Loop i Thermo Scientific Accela Open Autosampler User Guide 75 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 38 Standard Step 1 Aspirate rear air segment Solenoid Actuator Valve Pump 1 gt Pump2 gt Holding Loop Column 76 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 39 Standard Step 2 Get sample aspirate rear inject and front volume Solenoid Actuator Valve Pump gt Pump gt Holding Loop gt Column Thermo Scientific Accela Open Autosampler User Guide 77 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 40 Standard Steps 3 4 Aspirate front air segment Solenoid Actuator Valve Pump gt Pump2 Holding Loop Front air segment
39. acro Active Trays Tray Name Hardware configured tray name Stack Stack location of a given tray name Allow Use Click to switch between YES and blank YES means that the PAL system can access a given tray for obtaining samples use is allowed A blank in this row means that the PAL system cannot access the tray for obtaining samples use is not allowed Communications Com Port Select the computer port where you plug the autosampler communication cable Auto Select Instructs the system to automatically determine the computer port where you plug the autosampler communication cable Accela Open Autosampler User Guide 19 2 Getting Started Using the Autosampler Control Terminal If you need more fimware information please contact a local sales person for help Firmware Versions Supported For Accela Open Autosampler the only supported firmware version is 4 1 2 Using the Autosampler Control Terminal For general information on using the autosampler control terminal refer to the Accela Open Autosampler Hardware Manual To select the sample trays and to change the default pulse time setting to 4 0 seconds follow these procedures e Selecting the Tray Type e Changing the Pulse Time Setting 20 Accela Open Autosampler User Guide Thermo Scientific Selecting the Tray Type Thermo Scientific To select the tray type 2 Getting Started Using the Autosampler Control Terminal 1 On the Job Queue screen select
40. acro Default New Method efault amp l Variables Syringe m Macro Sequence LCi Save Template Save s Device Status lt unknown gt 36 Accela Open Autosampler User Guide Thermo Scientific 3 Creating Custom Templates and Macros Using the Template Editor to Create Custom Templates 2 Optional In the Template Description box type a description of your custom template 3 In the Syringe list select the appropriate syringe size to be specified in the template 4 Add macros to the template as follows a Under Macro select a macro from the list The Insert Above and Insert Below buttons become available b Click Insert Above or Insert Below The selected macro appears in the Macro Sequence list 5 Delete macros from the Macro Sequence list as follows a Select the macro that you want to delete from the Macro Sequence list b Click Delete 6 Replace a macro in the Macro Sequence list as follows a Under Macro select a macro from the list b Select the macro that you want to replace in the Macro Sequence list c Click Replace 7 Save the template a Click Save As The Save As dialog box appears b Select a file location c In the File name box type an appropriate name d Click Save The autosampler saves the template with the file extension pme Template Editor Page Use the Template Editor page of the Pal Template Macro Editor window to make a new template a sequence of
41. anufacturing location see the label on the instrument WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermo com WEEERoHS for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country WEEE Konformitat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96 EC erf llen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann Mehr Information ber die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific ber die Verwerter und weitere Hinweise die n tzlich sind um die Produkte zu identifizieren die unter diese ROHS Anweisung fallen finden sie unter www thermo com WEEERoHS Thermo SCIENTIFIC Conformit DEEE Ce produit doit tre conforme la directive europ enne 2002 96 EC des D chets d Equipements Electriques et Electroniques DEEE Il est marqu par le symbole suiva
42. ates for Accela Open AS with DLW could cause damage to the instrument and cause the injection to not perform any washes at all Accela Open Autosampler User Guide 27 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler WARNING If you select a DLW template make sure the Rear Volume Injection Volume Front Volume 2x Airgap is less than the syringe size Otherwise it will result in Error 86 Sringe Volume Out of Range In addition to these templates you can create your own custom templates see Using the Template Editor to Create Custom Templates on page 35 When you store a custom template in the same folder as the standard templates the template appears in the Template list Figure 19 shows the macro sequence and the macro variables for the Single Step LC Injection template 28 Accela Open Autosampler User Guide Thermo Scientific 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Figure 19 Method Setup page with LC Inj template Method Setup Method Summary Template Selection Template C Xcalibur System PAL Methads Single Step LC Injection pme Template Description Macro LCInj 1 of 1 Variables Air Volume fpl fo This is only a sample method for Pre Clean with Solvent 1 fo short runs For analytical work the method parameters must be set Pre Clean with Solvent 2 fo according to the specific needs Pre Clean with Sample fo Fili
43. c 3 Creating Custom Templates and Macros Using the Macro Editor to Create Custom Macros Using the Macro Editor to Create Custom Macros Macros are the building blocks that you use to create templates Creating a Custom Macro This section provides information about how to create a custom macro To create a custom macro 1 Open the Macro Editor dialog boxas follows a Open the Instrument Setup view for the autosampler b From the menu bar choose Accela Open AS gt Template Macro Editor c Click the Macro Editor tab The Macro Editor page of the Pal Template Macro Editor window appears see Figure 27 Thermo Scientific Accela Open Autosampler User Guide 45 3 Creating Custom Templates and Macros Using the Macro Editor to Create Custom Macros Figure 27 Macro Editor page of the Template Macro Editor window am Pal Template Macro Editor Aspirate File PAL View Help Deh Template Editor Macro Editor Template Summary Macro Command Aspirate v DISPENSE _DIL v Default All Parameters Dilutor m Macro Description Fill syringe from a vial or a wash station Volume ul Sample volume air volume filling Diutor Eject Speed SCS speed and filing strokes are Eject Speed l s Dilutor Eject Speed specified as parameters Dispense Delay ms bo Macro Variables Air Volume pl 0 0 SYR Max Volun A Filling Speed pl s SYR Fill Speed C aranda Vinh flOVD hdi
44. c Rotate the outer knob to move the cursor bar highlight to Objects and then press the inner knob button The Objects screen appears d Rotate the outer knob to move the cursor bar highlight to Out Signals and then press the inner knob button The Out Signals screen appears e Rotate the outer knob to move the cursor bar highlight to Injected and then press the inner knob button The Injected screen appears see Figure 15 2 Rotate the outer knob to move the cursor bar highlight to Pulse time and then press the inner knob button The time cell is highlighted 3 Rotate the outer knob to change the pulse time to 4 0 seconds and then press the inner knob button to set this selection as the default 4 Return to the Job Queue screen by pushing the F4 key Home Accela Open Autosampler User Guide 23 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Specifying the Instrument Method Parameters for the Autosampler To inject a sample set automatically you must create an instrument method that contains the chromatographic conditions and mass spectrometer data acquisition settings and then create an acquisition sequence that specifies the instrument method and vial location for each run This section describes how to specify the basic instrument method parameters for the autosampler For information about creating acquisition sequences refer to the Sequence Setup view Help
45. ct Control Configuration m Wrap each direct control method run with the following macros V Sequence Entry Exit V Method Entry Exit Method Search Paths Path Browse Add Delete Reset C Xcalibur Methads m Firmware Version 2 4 0 3 Add additional file locations as follows a In the Path box type the folder location or click Browse and browse to the appropriate folder to select it b Click Add The new folder location appears in the box below the row of buttons 4 Delete file locations as follows a Select the file location in the box below the row of buttons b Click Delete 5 To reset the location of the methods files to the default folder click Reset 60 Accela Open Autosampler User Guide Thermo Scientific 4 Using the Direct Controls to Operate the Autosampler Specifying the Location of the Direct Control Methods Direct Control Direct Control Configuration Page Thermo Scientific Use the Direct Control Configuration page in the Direct Control dialog box to enter the Direct Control Configuration information required for the autosampler to carry out autosampling Wrap each direct control method run with the following macros Table 12 Direct Control Configuration page parameters Parameter Sequence Entry Exit Description Use this check box to wrap methods with the sequence list entry and exit macros SLentry and SLexit Method E
46. ded on the LC Devices software CD is the device driver that enables the Xcalibur data system control of the autosampler This user guide provides you with information on how to connect the autosampler to other devices in the LC MS system and how to operate it from the Xcalibur data system This manual is provided in electronic format only You can access this manual by choosing the following from the computer desktop Start gt All Programs gt Thermo Instruments gt Manuals gt LC Devices gt Accela gt Accela OpenAS To provide us with comments about this document click the link below Thank you in advance for your help SURVEY Related Documentation The Accela Open Autosampler includes Help and these manuals as PDF files Accela Open Autosampler User Guide Accela Open Autosampler Hardware Manual Thermo Scientific Accela Open Autosampler User Guide ix Preface Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol IMPORTANT Highlights information necessary to prevent damage to software loss of data or invalid test results or might contain information that is critical for optimal performance of the system Not
47. e 0 0 99 Filling Speed pl s S R Fill Speed SYR Min Speed SYR Max Speed Filling Strokes 1 0 99 Inject to NJECTOR Injection Speed pl s SR Inject Speed SYR Min Speed SYR Max Speed Thermo Scientific Device Status Ready To Download Accela Open Autosampler User Guide 43 3 Creating Custom Templates and Macros Standard Macros Standard Macros Figure 26 shows the macros that are provided with a standard installation Figure 26 Standard macros C Xcalibur system PAL Wacros Edit ay File Back 7 t pa Search Folders O C ixcaliburisystem PAL Macros x aa Add to Sample and Mix pma a f Aspirate Sample pma E E Aspirate pma E Clean Syringe pma E Clean Yalve pma E Clear Output Signal pma E Dispense pma E Error Entry pma E Error Exit pma E Home pma E Inject pma LC Inj pma E Method Entry pma E Method Exit pma Pulse Output Signal pma E Reset pma E Set Output Signal pma E SL Entry pma 2P SL Exit pma E Switch Yalve to Inject pma Switch valve to Load pma E Transfer Liquid and Mix pma laa Transport Vial pma E Wait For Data System pma E Wait Signal pma y E Wait pma View Favorites Tools Help Eii JPE Address Folders O xcalibur data 4 O examples O Install Logs E methods E O Project E O system O PAL O Macros O methods O programs O temp O templates gt 44 Accela Open Autosampler User Guide Thermo Scientifi
48. e Highlights information of general interest Tip Highlights helpful information that can make a task easier Contacting Us There are several ways to contact Thermo Fisher Scientific for the information you need To contact Technical Support Phone 800 532 4752 Fax 561 688 8736 E mail us techsupport analyze thermofisher com Knowledge base www thermokb com Find software updates and utilities to download at mssupport thermo com To contact Customer Service for ordering information Phone 800 532 4752 Fax 561 688 8731 E mail us customer support analyze thermofisher com Web site www thermo com ms X Accela Open Autosampler User Guide Thermo Scientific Thermo Scientific Preface To copy manuals from the Internet Go to mssupport thermo com and click Customer Manuals in the left margin of the window To suggest changes to documentation or to Help e Fill out a reader survey online at http www surveymonkey com s aspx sm R7gKOvhLXn3NTkpK2BefHQ_3d_3d e Send an e mail message to the Technical Publications Editor at techpubs lcms thermofisher com Accela Open Autosampler User Guide xi o Y i Getting Connected The Accela Open Autosampler is a high throughput autosampler manufactured by CTC Analytics and supplied by Thermo Fisher Scientific For high throughput LC MS applications use the Accela Open Autosampler a Thermo Scientific or an Agilent liquid chromatography LC pump and a Thermo Sc
49. ected to the data system computer and turned on b Ifyou have already connected the autosampler to the data system computer click Check to verify the connection 6 To shorten the time between injections select the Enable check box under Look Ahead Injections When you enable Look Ahead Injections the autosampler sets up for the next injection during the current run The setup for the next injection involves carrying out the steps up to but not including the injection Tip Do not modify or delete a sequence while the data system is running with the Look Ahead Injections enabled Use the stop function to finish the current injection before you modify or delete a sequence 7 To make the Method Template and Macro Editor dialog boxes available in the Instrument Setup window select the Enable check box under Method Template Macro Editor Thermo Scientific Accela Open Autosampler User Guide 17 2 Getting Started Adding the Autosampler to the Xcalibur Instrument Configuration 8 In the Cycle Composer Macro Importer area import the macros that you plan to use to create injection methods a In the Import Path box type the location where the macros reside Or click Browse to locate the directory that contains the appropriate macros b In the Overwrite Existing Macros list make one of the following selections e Never The autosampler does not import the specified macro if it has the same file name as an existing macro
50. elay time longer than the method run time the autosampler begins the next injection at the end of the current run This means that adding a long delay time does not add additional run time to the method run time but it does cancel the effect of using the Look Ahead Injections feature Note The Look Ahead Injections feature is available if you selected the Enable check box in the Look Ahead Injections area of the Pal Configuration dialog box Specify the acquisition parameters for the other devices of your LC or LC MS instrument 30 Accela Open Autosampler User Guide Thermo Scientific 11 Save the method 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler a From the Instrument Setup window menu bar choose File gt Save As The Save As dialog box appears b Select an appropriate file location for the method c In the File name box type an appropriate name for the method d Click Save The File Summary Information dialog box appears e Optional In the Comment box type additional information about the method f Click OK Xcalibur or other Thermo Scientific data acquisition applications such as LCquan stores the method as a meth file in the specified file location Instrument Setup Parameters Thermo Scientific Use the Method Setup page to specify instrument method parameters for the autosampler Table 5 Method Setup page parameters Sheet 1 of 3 Parameter Template
51. et Direct Control Thermo Pal Accela Pump Methods Tray Configuration Direct Control Configuration Method Vial Index Latanoprost 7 fis Description _ fi Duplication of PAL local LC Inj cycle This is only a sample method for 1 CStk1 01 v short runs For analytical work the method parameters must be set 2 Cstk1 01 according to the specific needs Trays v Volume gt 1 130 2 No m Injectors Syringe 100ul m Device Status Ready To Download 7 Make the following selections and entries e Under Vial Index type the location of the sample vial e Under Trays select the tray where the sample vial is located e Under Volume type the volume that you want the autosampler to inject 8 Click the tab for the configured LC pump and start the solvent flow from the LC pump Note Thermo PAL only downloads the method parameters for the autosampler It ignores the method parameters for the LC pump and the MS detector 9 Return to the Methods page for the Thermo Pal and click Run 56 Accela Open Autosampler User Guide Thermo Scientific Direct Control Methods Page Thermo Scientific 4 Using the Direct Controls to Operate the Autosampler Making Single Injections from the Tune Window The Methods page of the Inlet Direct Control dialog box has the following parameters Table 10 Methods page parameters
52. etting Started This chapter describes how to add the autosampler to the Xcalibur instrument configuration how to specify the available tray types from the Accela Open Autosampler control terminal and how to specify the injection parameters for the autosampler from the Xcalibur data system Contents e Adding the Autosampler to the Xcalibur Instrument Configuration e Using the Autosampler Control Terminal e Specifying the Instrument Method Parameters for the Autosampler Adding the Autosampler to the Xcalibur Instrument Configuration The Accela Open Autosampler device driver provides control of the autosampler from the Xcalibur data system To control the autosampler from the data system add the autosampler to the Xcalibur instrument configuration and specify the data system COM port that the autosampler is connected to Specifying the Configuration Options This section provides information about how to specify configuration options for the autosampler 1 To specify the configuration options for the autosampler 1 Open the Instrument Configuration window from the computer desktop as follows For Xcalibur 2 1 0 or later versions choose Start gt All Programs gt Thermo Foundation 1 0 gt Instrument Configuration The Unloading Device Drivers dialog box appears followed by the Thermo Foundation Instrument Configuration window see Figure 12 Thermo Scientific Accela Open Autosampler User Guide 15 2 Getting Started Add
53. front view of the Accela Open Autosampler During installation a Thermo Fisher Scientific field service engineer connects the solvent lines between the injection valve and the LC pump and between the injection valve and the Thermo Scientific mass spectrometer Figure 1 Accela Open Autosampler System Synchronization Connections Thermo Scientific The system interconnect cables and adapter cables that synchronize the run signals for an LC or LC MS system with an Accela Open Autosampler depend on the Accela pump model the mass spectrometer model and whether the LC system includes an Accela detector Table 3 lists the system interconnect and adapter cables that Thermo Fisher Scientific supplies with the Accela Open Autosampler or by special order Accela Open Autosampler User Guide 3 1 Getting Connected System Synchronization Connections 4 Table 3 Accela Open Autosampler contact closure and adapter cables Cable description Accela detector and MS detector adapter cable Part number 60157 63026 Use Required for hardware configurations that include one or more of the following e Accela detector app deid witi e TSQ or Exactive mass spectrometer autosampler Accela Open 60157 63024 Required for all hardware configurations Autosampler system interconnect cable supplied with autosampler Accela Pump adapter cable available by special order 60157 63022 Required for the Accela Pump
54. g box that opens when you click Add The Edit Parameter dialog box has a check box for the Parameter Type numeric or object If you select a numeric Parameter Type you see a pane for entering the new parameter name a list for the parameter units and panes for entering the default value for the parameter along with the lower and upper limits for the parameter If you select an object Parameter Type you see a check list of objects that might be represented by the parameter Thermo Scientific Accela Open Autosampler User Guide 47 3 Creating Custom Templates and Macros Using the Macro Editor to Create Custom Macros Table 8 Macro Editor page parameters Sheet 2 of 3 Parameter Edit Description Edits an existing macro variable The editing is carried out in an Edit Parameter dialog box that opens when you click Edit The Edit Parameter dialog box has a check box for the Parameter Type numeric or object If you select a numeric Parameter Type you see a pane in which you enter the new parameter name a list for the parameter units and panes in which you enter the default value for the parameter along with the lower and upper limits for the parameter If you choose an object Parameter Type you see a check list of objects that may be represented by the parameter Delete Command Sequence Deletes the selected Macro Variable The sequence of commands that make up the macro selected in the Macro list Double clickin
55. g one of the commands highlights it in blue and causes its display in the Command area In the Command area the command parameters are shown in both panes continuous variables and lists discrete variables Insert Above The command shown in the Command area is inserted above the command selected highlighted in blue in the Command Sequence pane Insert Below The command shown in the Command area is inserted below the command selected highlighted in blue in the Command Sequence pane Replace The command selected highlighted in blue in the Command Sequence pane is replaced by the command shown in the Command area Delete 48 Accela Open Autosampler User Guide Deletes the command selected highlighted in blue in the Command Sequence pane Thermo Scientific 3 Creating Custom Templates and Macros Using the Macro Editor to Create Custom Macros Table 8 Macro Editor page parameters Sheet 3 of 3 Parameter Description Command Individual autosampler commands are displayed in a list These individual commands make up the command sequence of a autosampler macro Select the command by moving the cursor over the command no click is needed highlighting each command in blue Clicking the command displays it in this list highlighted in blue Simultaneously the command parameters are displayed in the Parameters area below the Command list At the bottom of this area below the Parameters area is a pa
56. g the Direct Controls to Operate the Autosampler Making Single Injections from the Tune Window Making Single Injections from the Tune Window Thermo Scientific You can make single injections from the Tune window K2 bd I 2 To make single injections from the Tune window Open the Tune window for your mass spectrometer Open the Inlet Direct Control dialog box see Opening the Direct Control Dialog Box on page 53 Specify the location of the methods that you want to use see Direct Control Tray Configuration Page on page 59 Specify the tray types that are loaded in the autosampler L tray holders see Changing the Tray Configuration on page 58 Click the Methods tab The Methods page appears The Syringe area lists the size of the configured syringe The Device Status area lists the status of the autosampler In the Method list select a method A description of the method appears in the Description box see Figure 31 on page 56 The method list depends on the file locations specified on the Direct Control Configuration page Note The visible DLW methods in the Method dropdown list are only available if you have installed the DLW option that comes with your automsampler Accela Open Autosampler User Guide 55 4 Using the Direct Controls to Operate the Autosampler Making Single Injections from the Tune Window Figure 31 Methods page of the Inlet Direct Control dialog box Inl
57. he recommended injection volume and the variables specified in the LC Inj macro IMPORTANT Make sure that your syringe selection matches the size of the syringe that is installed in the autosampler e Ifthe autosampler is set up to recognize the installed syringe size an error message appears when you download methods that specify a different syringe size Ifthe autosampler is not set up to recognize the installed syringe and the specified syringe size does not match the actual syringe size the autosampler does not inject the specified injection volume Under Recommended Injection Volume in the Volume UL box type the volume of sample that you want the autosampler to inject The allowable injection volume range is based on the syringe size IMPORTANT For partial loop injections variable volume make sure that the recommended injection volume is less than half the sample loop size Under Look Ahead Injections if the Delay Time mins box is available type the amount of time that you want the autosampler to wait after it completes the current injection before it starts the next injection During a look ahead injection the autosampler aspirates the sample for the next injection and waits until the end of the current run to inject the sample into the valve When you add a delay time the autosampler does not begin the next injection cycle immediately after completing the current injection cycle If you enter a d
58. ides information about how to create custom variables To create custom variables 1 Open the Macro Editor dialog box as follows a Open the Instrument Setup view for the Accela Open Autosampler see Specifying the Instrument Method Parameters for the Autosampler on page 24 b From the menu bar choose Accela Open AS gt Template Macro Editor c Click the Macro Editor tab The Macro Editor page of the Pal Template Macro Editor window appears see Figure 27 2 Click Add The Variable Definition dialog box appears see Figure 28 Figure 28 Variable Definition dialog box Variable Definition Variable Type OK Cancel Variable Name sa __ Cancel Variable Units Help Default Value Lower Limit Upper Limit 3 Select a variable type e To create a variable that is a numeric value select the A Number Value option Then go to step 4 e To create an object variable select the An Object option A list of objects appears Go to step 5 50 Accela Open Autosampler User Guide Thermo Scientific Thermo Scientific 3 Creating Custom Templates and Macros 4 Complete the definition of a numeric variable as follows e f In the Variable Name box type an appropriate name In the Variable Units list select a unit of measure Defining Variables In the Default Value box type a default value for the variable In the Lower Limit box type a lower limit for the variable In the Upper Lim
59. ientific mass spectrometer MS detector This chapter describes the contact closure connections between the Accela Open Autosampler one or two Thermo Scientific LC pumps or an Agilent pump a Thermo Scientific MS detector and the Xcalibur data system computer CAUTION Follow all of the recommendations given in the Safety Information section of the CTC Analytics PAL System User Manual The Safety Information section includes information on the Accela Open Autosampler electrical hazards lithium battery and safety labels Contents e Ordering Information e System Synchronization Connections e System Synchronization Connections e Connecting the Accela Open Autosampler to the Data System Computer Ordering Information The Accela Open Autosampler options that Thermo Fisher Scientific supplies include the serial communication cable that you use to connect the Accela Open Autosampler to the data system computer and the contact closure cable that you use to connect the Accela Open Autosampler to a Thermo Scientific LC pump and a Thermo Scientific MS detector Thermo Scientific Accela Open Autosampler User Guide 1 1 Getting Connected Setting Up an LC MS System with the Autosampler Table 1 lists the Accela Open Autosampler options that you can order from Thermo Fisher Scientific Table 1 Accela Open Autosampler ordering information Part number Description OPTON 13008 HTS PAL OPTON 13009 HTC PAL OPTON 13010 HTC PAL with
60. ing the Autosampler to the Xcalibur Instrument Configuration Figure 12 Thermo Foundation Instrument Configuration window 22 Thermo Foundation Instrument Configuration Device Types All Available Devices Configured Devices Accela UVAVIS Accela Open AS Agilent1100 4S Agilent 100 Thermostatted AS Le Agilent 100 WellPlate Agilent 100 Bin AS 2 In the Device Types list select All 3 In the Available Devices list double click the autosampler A copy of the Accela Open AS icon appears in the Configured Devices list 4 In the Configured Devices list double click i Accela Open AS The Pal Configuration dialog box appears see Figure 13 16 Accela Open Autosampler User Guide Thermo Scientific 2 Getting Started Adding the Autosampler to the Xcalibur Instrument Configuration Figure 13 Pal Configuration dialog box Pal Configuration Communications Com Port aa Auto Select Cheol al p Look head Injections r Method Template Macro Editor Enable E Enable v Cycle Composer Macro Importer Import Path C Overwrite exisiting macros Never Import Browse m Active Trays 5 Select the data system computer Com port that the autosampler is connected to a In the Com Port list select the Com port that the autosampler is connected to Or click Auto Select Note The autoselect feature works only if the autosampler is conn
61. ing the F4 key Home Changing the Pulse Time Setting To control LC systems with a Thermo Scientific LC pump and an autosampler from the Xcalibur data system you must change the default pulse time setting for the autosampler from 2 to 4 seconds The pulse time is the length of time that the autosampler sends an output signal after it makes an injection The LC pump receives this output signal through the contact closure cable IMPORTANT If your LC system contains a Thermo Scientific LC pump and an autosampler you must change the autosampler s default pulse time setting If you leave the default pulse time set to 2 seconds the status of the LC pump remains at waiting for contact closure after you make an injection 22 Accela Open Autosampler User Guide Thermo Scientific Thermo Scientific 2 Getting Started Using the Autosampler Control Terminal Change the pulse time setting to 4 0 seconds from the Injected screen see Figure 15 Figure 15 Menu path to Injected screen Menu Setup Objects gt p Out Signals Tels Destination SW Out1 Pulse Time Oms 9 9s Set Defit Home To change the pulse time setting 1 Open the Injected screen as follows a On the Job Queue screen select Menu by pressing the F1 key see Figure 14 b Rotate the outer knob to move the cursor bar highlight to Setup and then press the inner knob button The Setup screen appears
62. irect Control dialog box opening 53 DLW characterizing features 64 cycle 75 cycle for fast injection 97 cycle for standard injection 75 fast injection cycle 70 macro definitions 65 operating 72 overview 63 priming solvent lines 66 standard injection cycle 68 DLW Actuator Solenoid 73 DLW Pumps 73 Thermo Scientific E electromagnetic compatibility iii F FCC compliance iii file types instrument methods meth 31 macro pma 47 templates pme 37 ICC cycles 64 Injected screen on hand held controller 23 Inlet Direct Control opening 54 Instrument Configuration window 15 Instrument Setup window opening 24 J Job Queue screen 21 L level surface importance of 2 lithium battery 1 look ahead injections 17 Macro Editor enabling 17 opening 45 macros creating 45 importing 18 list of standard 44 Menu screen 21 method parameters specifying 24 MSQ Plus MS detector Accela Open Autosampler system interconnect cable Accela Open Autosampler User Guide 109 Index N connection 5 8 numeric variables 51 0 object variables 51 ordering information 2 P part numbers 2 priming solvent lines 66 pulse time of the out signals setting 23 pumps self priming 64 Recommended Injection Volume box 30 regulatory compliance iii S safety information 1 safety standards iii self priming micro pumps 64 solvent lines priming 66 solvent lnes priming 72 syringe size specifying 30
63. it box type an upper limit for the variable Click OK to save the variable and close the Variable Definition dialog box 5 Complete the definition of an object variable as follows a b C In the Variable Name box type an appropriate name Select the appropriate objects see Figure 29 Click OK to save the variable and close the Variable Definition dialog box Figure 29 Object variable selections Variable Definition Variable Type C A Numeric Value Variable Name Please check what objects this variable may be SYRINGE P VIAL TRAY_TYPE TRAY P WASH_STATION INJECTOR AGITATOR MOTOR_DRIVE HEATER PATH l SYNC_SIGNAL F OUT_SIGNAL QUT_EVENT J Sw_EVENT PwR_EVENT M MOTOR_X M MOTOR_Y M MOTOR_Z USER M DILUTOR SER_VALVE Cancel Help TRAY_HOLDER F VALVE POSITION IN_EVENT R9232 CHKMOTOR M OUT_EXP Accela Open Autosampler User Guide 51 3 Creating Custom Templates and Macros Defining Variables Variable Definition Dialog Box Use the Variable Definition dialog box to specify the details of a numerical or object Macro Variable that you want to add or edit in the Macro Variables box Table 9 Variable Definition dialog box Parameter Description Variable Type A Numeric Value Select this if the parameter is a numeric value If you are editing an existing numerical parameter this box is marked with a black dot An Object Select this if the
64. k between the two ports Flushing the valve with both wash solvents eliminates remaining sample material located between stator ports 1 and 6 What points must you consider when you use the Stator Wash or Valve Toggle option Observe the rules if biofluid samples are injected First sample contact should always be with an aqueous solution to avoid protein precipitation After washing with organic solvent higher elution power flush the system again with wash solvent 1 The first toggle near the end of the chromatographic cycle provides the advantage that the sample loop is already flushed out first with the mobile phase with a solvent of high elution power assuming gradient application Accela Open Autosampler User Guide 89 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step The second valve toggle time follows immediately after finishing the second solvent flush You cannot program a second switching time The waiting time for the second valve toggle should be long enough so that the entire system is flushed out by both wash solvents Consider the entire delay volume to determine the second valve switch The DLW internal volumes are as follows Manifold 90 pL Holding Loop 108 pL Syringe Needle Gauge 22 6 7 pL Installed Injection Loop Total delay volume 205 pL Loop content volume Do the second valve toggle back to starting condition before the system equilibration time has started The Loop content is ideally a s
65. macros Each macro in the sequence is made up of variables You may also edit these variables on this page Table 6 Template Editor page parameters Sheet 1 of 3 Parameter Description Template Description Enter the method description in this box Syringe Select the syringe volume from this list Thermo Scientific Accela Open Autosampler User Guide 37 3 Creating Custom Templates and Macros Using the Template Editor to Create Custom Templates Table 6 Template Editor page parameters Sheet 2 of 3 Parameter Macro Sequence Description This box displays the macros chosen for the method being made in the Method Template Editor The macros in this sequence are selected from the Macro list see below Double clicking a macro in the Macro Sequence box displays it in the Macro list as described later in this table along with the variables that make up the macro in the Variables area below the Macro list Insert Above A Macro in the Macro list see below is placed above the macro selected in the Macro Sequence box highlighted in blue Insert Below A Macro in the Macro list see below is placed below the macro selected in the Macro Sequence box highlighted in blue Replace Replaces the macro highlighted in blue in the Macro Sequence box with the macro that is in the Macro list see explanation below Delete Deletes the macro that is selected highlighted in blue in the Macro Sequence
66. mo Scientific 5 Using Dynamic Load and Wash DLW Operating Dynamic Load and Wash DLW Location of Solvent and Waste Bottles DLW Pumps The DLW option contains self priming membrane pumps You can place the solvent bottles either in the fast wash station holder or on the lab bench You must place the Waste bottle greater than 30 cm 11 8 in below the injection valve Make sure that the waste liquid can flow into the waste bottle without restriction Place the waste tubing above the level of the liquid Ideally the tube is fixed at the neck of the waste bottle Note Use good laboratory practice to avoid contaminating the wash solvents and the wash bottles Avoid biological growth in pure water by either replacing it regularly or adding a small percentage of organic solvents such as methanol or acetonitrile Certain buffer solutions can decompose at room temperature when exposed to light Filtering the wash solvents before filling the bottle especially if using salt buffers is mandatory to avoid any clogging of the solvent paths From the control point of view the DLW pumps respond in the same manner as the fast wash station Power out signals activate the pumps Because the electric current setting for the DLW is different you must load the corresponding PAL Firmware Objects for the DLW wash station type The wetted parts in the pump are made from the following materials e Membrane Kalrez FFPM e Body valves Ryton PPS
67. mp the Needle Gap and the Rinse Time are selectable Macro variable Needle Gap is a parameter from Rinse Inj Atom The variable in this macro is Needle Gap Valve Clean The function of this parameter is to raise the needle in the injection port very little to allow rinsing around the needle tip The pressure of the spring loaded balls in the DLW Syringe Holder assembly is released by moving approximately 3 mm up default This leaves a gap between the needle tip and the valve bottom of approximately 1 mm to enable a flush at this contact point A Repeat End loop enables adding an extra rinsing step valve toggle Stator Wash Counter 0 disable valve toggle steps Counter 1 enable valve toggle steps If the counter is set to 1 follow the described steps below If the counter is set to 0 the macro finishes at this point The injection moves to the injection valve The valve is switched to the Active position The valve is rinsed with Wash Solvent 2 followed by Wash Solvent 1 Stator Wash Time Solvent 2 Stator Wash Time Solvent 1 Remark The loop is filled with the last rinse with Wash Solvent 1 Verify the composition of Wash Solvent 1 The solvent should have a lower elution power than the solvent gradient starting conditions or sample solvent composition This is important for partial loop filling The injection valve is switched back to the Standby position End of macro DLW Priming
68. mponents options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives FCC Compliance Statement THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS 1 THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE AND 2 THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION CAUTION Read and understand the various precautionary notes signs and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device Notice on Lifting and Handling of Thermo Scientific Instruments For your safety and in compliance with international regulations the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and or move the instrument This instrument is too heavy and or bulky for one person alone to handle safely Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For m
69. n folder on the CD ROM to the method folder If the PAL system is integrated in a data system software that controls the PAL using the Cycle Editor for PAL ICC interpretation for example Analyst ChemStation Empower EZChrom MassLynx Xcalibur an ICC Cycle is used and not the Cycle Composer Macro The cycle extension is cyx Note You can convert a Cycle Composer macro to a cycle extension cyx by using the Cycle Editor software Conversion is available starting with Cycle Editor version 1 4 0 4 Note The provided macros are written for standard injection valve drives which are controlled and activated through the AUX interface Accela Open Autosampler User Guide 65 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles General Considerations You must establish the duration of the wash steps for each configuration and application Consider factors such as the viscosity and surface tension of the individual wash solvent composition and the backpressure of the system Be aware that a higher backpressure builds up if the valve bore size standard valve bore 0 25 mm or the installed loop internal diameter is lower Standard loop internal diameter ID for Thermo defined loops with a volume of 5 10 and 20 pL is 0 25 mm The loop with 2 pL content volume has an ID of 0 125 mm Keep the tubing internal diameters of the tubing in line with the valve dimensions loop ID a
70. n its box Save Template Saves the Method Template under the current file name overwrites the current file Save As Saves the Method Template under a new file name or in a new location that you type in the Save As dialog box Testing a Custom Template This section provides information about testing a custom template Using the Template Editor Dialog Box to Test a Custom Template You can test a custom template from the Template Editor dialog box KD To test your new template step by step 1 If the Template Editor dialog box is closed open it 2 Open the template that you want to review by choosing File gt Open Method Template The default path to open a template is as follows drive Thermo Instruments LC Devices AccelaOpenAS PAL methods Thermo Scientific Accela Open Autosampler User Guide 39 3 Creating Custom Templates and Macros Testing a Custom Template 3 Click Step The Run Auto Sampler Method In Step Mode appears see Figure 22 Figure 22 Run Auto Sampler Method In Step Mode dialog box Run Auto Sampler Method In Step Mode Vial Index Injectors 1fi 1 Lev 2 f 2 Leyi X Trays Volume 1 CStk1 01 1 10 2 CStk1 01 v 2 fio Injections Per Vial ooo Run Cancel 4 Make the appropriate entries and selections e Under Trays select the appropriate trays e Under Vial Index type an appropriate vial location for the template you are testing e Under Volume type the volume
71. nd flow rate Priming the Solvent Lines Wash1 and Wash2 This macro is used at installation to prime the entire system Set the wash time to approximately 120 seconds for each solvent After installation for best results prime the system before activating the first run For daily preparation of the system the wash time can be much shorter approximately 20 seconds The goal is an entire liquid system free of any air bubbles Table 14 lists the Priming Accela Open AS_Rev01 macro parameters Table 14 Priming Accela Open AS_Rev01 macro Sheet 1 of 2 Macro description Macro variable The injection unit moves to the DLW Wash Clean Time solvent 1 Station position Wash1 Eject Speed DLW Syringe The injection unit moves to the DLW Wash Clean Time solvent 1 Station position Wash2 Eject Speed DLW Syringe The DLW system is rinsed with Wash Solvent 1 in position Waste 66 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Table 14 Priming Accela Open AS_Rev01 macro Sheet 2 of 2 Macro description The injection valve is cleaned first with the content of DLW syringe Wash Solvent 1 followed by Wash Solvent 2 and finally the last wash to prepare the system for injection cycle rinsed with Wash Solvent 1 again Remark The Atom Rinse Inj is new available starting with FW 4 1 x The DLW actuator solenoid is activated the Wash Solvent pu
72. ne that describes the function of the selected command Parameters The Parameters area displays the command parameters used by the autosampler command that you select in the Command list Parameters with specific allowed values discrete parameters are displayed in lists Continuous parameters are displayed in panes You may choose the allowed values of discrete parameters from those presented in the list for the parameter The allowed values for continuous parameters are displayed when you hold the cursor over the respective pane for the parameter You can type the value for the continuous parameter in its pane and view other allowed continuous parameter values in a shortcut menu You activate this menu by right clicking the continuous parameter box of interest The parameter you select from the shortcut menu appears in the box Command Description A description of the command displayed in the Command list Save Macro Saves the macro command sequence under the current file name overwrites the current file Save As Saves the macro command sequence under a new file name in a new location or with both a new file name and location that you type in the Save As dialog box Thermo Scientific Accela Open Autosampler User Guide 49 3 Creating Custom Templates and Macros Defining Variables Defining Variables Variables are the building blocks that you use to create macros Creating Custom Variables This section prov
73. ng Speed pls 50 TEF Filing Strokes f Syringe 100ul z Inject to LC Viv v Injection Speed pl s f oo Pre Inject Delay ms 500 Post Inject Delay ms 500 Post Clean with Solvent 1 f Post Clean with Solvent 2 fo Valve Clean with Solvent 1 f Valve Clean with Solvent 2 f Duplication of PAL local LC Inj cycle Macro Sequence Recommended Injection Voluine Volume ul j 0 000 Look Ahead Injections Delay Time mins 0 00 Help Default All Macro sequence for the Variables list Single Step LC Injection template When you select a template the macros used by the template appear in the Macro Sequence area When you click on a macro listed in the Macro Sequence area the variables used by the Macro appear in the Variables area 6 In the Variables area keep the parameters set to the defaults or type the appropriate values for your application Thermo Scientific Accela Open Autosampler User Guide 29 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Ta 10 In the Syringe list select the syringe size that is installed in the autosampler The default variable settings for the Single Step LC Injection template and the default setting of 10 uL in the Recommended Injection Volume box are suitable for a 100 uL syringe Changing the syringe size can change the allowable ranges for t
74. nt Thermo Fisher Scientific s est associ avec une ou plusieurs compagnies de recyclage dans chaque tat membre de Punion europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Fisher Scientific 4 ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la d tection des substances sujettes la directive RoHS sont disponibles sur www thermo com WEEERoHS a a E Contents Prelate eC ee ere rer er er ee ee ix Related Documentation s 209s nese srun nuren nods aie eas EOS wes ix Satctand Special Notes cust dee es due enric sareek enwbieinanh ee x Contacting US er issa cate vee eed tiaa ias EAEI ERRE E ae ee as x Chapter1 Getting Connected 0 cece cece eee eee eee eae 1 Ordering Informati n essers eseng tE deniar E EENE TE OEA GRESS 1 Setting Up an LC MS System with the Autosampler 00 2 System Synchronization Connections 00 cece eect eee ene 3 Connecting the Accela Open Autosampler Interconnect Cable 5 Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector via2ccetlaceiee dee kia aa tee etka eee 2 cee hea 7 Connecting the Accela Pump Adapter Cable 0 0 0 00000 e eee 11 Connecting the Accela Open Autosampler to the Data System Computer 14 Chapter2 Getting Started 0 0c cece eee eee eee
75. ntry Exit Use this check box to wrap methods with the method entry and exit macros Method Entry and Method Exit Method Search Paths Path Use this box to specify the extra path you want added to the method search routine Browse Use this button to search for a path Add Use this button to add the current path to those searched Delete Use this button to delete the currently selected path Reset Use this button to remove all of the user added paths text box This text box displays the directories that the autosampler searches for methods Firmware Version Specifies the firmware version downloaded from the autosampler Accela Open Autosampler User Guide 61 4 Using the Direct Controls to Operate the Autosampler Wrapping Direct Control Injections with Additional Macros Wrapping Direct Control Injections with Additional Macros 62 Use the Direct Control Configuration dialog box to specify whether the autosampler is to run the SL Entry and SL Exit the Method Entry and Method Exit or all of these macros before and after an injection To specify the use of the SL Entry and SL Exit macros Select the Sequence Entry Exit check box To specify the use of the Method Entry and Method Exit macros Select the Method Entry Exit check box Accela Open Autosampler User Guide Thermo Scientific ee e Using Dynamic Load and Wash DLW This chapter describes how to use the Dynamic Load and Wash DLW option which include
76. olvent of a low elution power when switched back If you apply isocratic chromatography the remaining contaminants might be washed into the system and can build up higher background noise for the column the detector or both over a longer period of time 90 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Additional Cleaning Step Stator Wash or Valve Toggle Step by Step Figure 52 to Figure 57 illustrate additional cleaning steps for Stator Wash Stator Wash End of Standard Injection Cycle Figure 52 Start for additional cleaning step Valve Toggle Solenoid Actuator Valve Pump 1 lt gt E gt column Thermo Scientific Accela Open Autosampler User Guide 91 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 53 Stator Wash Step 1 2 Valve switched to Load Position toggle valve is cleaned with Wash Solvent 1 Solenoid Actuator Valve Column Pump 92 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 54 Stator Wash Step 3 Valve is cleaned with Wash Solvent 2 Solenoid Column Pump Thermo Scientific Accela Open Autosampler User Guide 93 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 55 Stator Wash Step 4 Wash Solvent 2 dispensed by Wash Solvent 1 Solenoid Actuator Valve Column
77. or the next injection Wash1 Inject to Needle Gap Valve Clean Valve Clean Time Solvent 1 The injection unit is moved back to the DLW Wash Station Wash1 position to flush the syringe needle inside and out with Wash Solvent 1 This is a preparation step for next injection and especially important for biofluid samples Wash 1 Post Clean Time Solvent 1 Cycle end for LC Inj DLW Standard macro Accela Open Autosampler User Guide 69 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Table 15 Stand Injection Accela Open macro parameters Sheet 3 of 3 Macro description An optional cleaning step is attached to the DLW Standard injection cycle Stator Wash or valve toggle A Repeat End loop can be activated with the Count Macro variable Stator Wash Stator Wash count 1 Cleaning step active Stator Wash count 0 Cleaning step disabled If Stator Wash is activated the following steps will be executed The injection unit is moved to the injection valve From the last step above the Holding Loop is filled with Wash Solvent 1 Timer 1 is awaited to switch the valve Toggle into Active position fill loop The DLW Actuator Solenoid is activated to deliver Wash Solvent 2 to the Holding Loop and into the valve system The first solvent flush arriving at the valve is Wash Solvent 1 parked in the Holding Loop at the beginning followed by Wash Solvent 2
78. parameter is an object If you are editing an existing object parameter this box is marked with a black dot Variable Name If you are editing an existing variable this box shows the name of the variable Type your choice for the name of the variable if you are adding a new one OK Accepts the new Variable Definition or accepts the edits to an existing Variable Definition Cancel Deletes changes made in the Variable Definition dialog box Numeric Value Variable Type Variable Units If you are editing an existing variable this list shows the units for the selected variable If you are adding a new variable you may enter units appropriate for that variable When you click the list arrow you can select the appropriate units from the options in the list Default Value The nominal or center value of the variable Lower Limit The lowest acceptable value of the variable Upper Limit The highest acceptable value of the variable Object Variable Type Please check what objects this variable may be 52 Accela Open Autosampler User Guide Select one or more objects that are described by the variable Thermo Scientific M Using the Direct Controls to Operate the Autosampler This chapter describes how to operate the autosampler from the Direct Control dialog box Contents e Opening the Direct Control Dialog Box e Making Single Injections from the Tune Window e Changing the Tray Configuration
79. pen Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Contact closure connections back panel for a standalone Accela LC system with an Accela Open Autosampler Figure 6 Accela Open Interconnect cable aa a a s Accela PDA Detector N ae t INTERFACE 1 0 l l d e Accela UV Vis Detector 1 e 7 sa SS Te ee 77 N Ne N g Accela 600 Pump or I Accela 1250 Pump pa Ra aes obs ra N TS a x 7 S Ss N 7 I D SUB25 a a l C Sim Teine I 3 A ZY n a eS a 7 en Adapter cable DAR Accela Open Autosampler User Guide 9 Thermo Scientific 1 Getting Connected System Synchronization Connections Figure 7 Accela Open Autosampler interconnect cable and detector adapter cable connections MSQ Plus Mass Detector back panel _ ow pae E N 7 USER I O N Startins Start In Ready Out Ready Out Accela Open Autosampler back panel et song f 7 s T Ei 5 pn m aj j E LTO Series mass spectrometer right panel
80. pler User Guide Thermo Scientific Thermo Scientific 2 Getting Started Adding the Autosampler to the Xcalibur Instrument Configuration Table 4 Pal Configuration dialog box parameters Sheet 2 of 2 Parameter Auto Select Description Instructs the system to automatically determine the computer port connected to the autosampler through the autosampler communication cable Check Instructs the system to verify the computer port connected to the autosampler through the autosampler communication cable Look Ahead Injections Enable Selecting this check box enables Look Ahead Injections When Look Ahead Injections is enabled the autosampler sets up for the next injection during the current run The setup for the next injection involves carrying out the steps up to but not including the injection Method Template and Macro Editor Enable Select this check box when you want the Method Template and Macro Editor to be accessed through Instrument Setup Ensuring the check box is clear prevents access to the Method Template and the Macro Editor Cycle Composer Macro Importer Import Path Enter the path to the Cycle Macro to be imported Overwrite existing macros Choices are Never Ask Always Uses chosen action to determine if existing macros are to be overwritten when a new macro is imported Import Import the chosen macro Browse Search through files and folders to find a m
81. re 46 Standard Steps 13 14 Valve clean with Wash Solvent 2 Solenoid Actuator Valve gt Column 84 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 47 Standard Steps 15 16 Active syringe needle wash with Wash Solvent 2 Solenoid Actuator Valve Column Thermo Scientific Accela Open Autosampler User Guide 85 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 48 Standard Steps 17 18 Valve clean with Wash Solvent 1 Solenoid Actuator Valve Pump gt Pump2 gt Column 86 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 49 Standard Steps 19 20 Active syringe needle wash with Wash Solvent 1 Solenoid Actuator Valve Column Thermo Scientific Accela Open Autosampler User Guide 87 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Figure 50 Standard Cycle end Solenoid Actuator Valve Pumpi gt Pump gt Holding Loop gt Column a Additional Valve Toggle Step to DLW Standard Cycle This section contains information about additional steps that are necessary for a DLW Standard Cycle 88 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW DLW Cycle Step by Step Considerations for Additional Stator Wash Cleaning Step
82. s on page 35 2 Open the template that you want to review by choosing File gt Open Method Template The default path to open a template is as follows drive Thermo Instruments LC Devices AccelaOpenAS PAL methods 3 Click the Template Summary tab Accela Open Autosampler User Guide Thermo Scientific 3 Creating Custom Templates and Macros Viewing the Template Summary The Template Summary page appears with a summary of the active template see Figure 25 Figure 25 Template Summary page Pal Template Macro Editor File PAL view Help Dl a ze Template Editor Macro Editor Template Summary Description Default New Method Syringe 100ul Macro Sequence LC Inj Step 1 of 1 Air Volume pl 0 Pre Clean with Solvent 1 0 Pre Clean with Solvent 2 0 Pre Clean with Sample 0 Filling Speed pls 50 Filling Strokes 1 Inject to LC Vlv1 Injection Speed pl s 100 Pre Inject Delay ms 500 Post Inject Delay ms 500 Post Clean with Solvent 1 1 Post Clean with Solvent 2 1 Valve Clean with Solvent 1 1 Valve Clean with Solvent 2 1 Macros used within method Macro name LC Inj COMPLETE CYCLE XXXXXXXXXXXXXXXXXXXXXXXX Duplication of PAL s local LC injection cycle Tray Name Sample Number and Sample Yolume are taken from the sample list Variables 14 Air Volume pl 0 0 SYR Max Yolume Pre Clean with Solvent 1 0 0 99 Pre Clean with Solvent 2 0 0 99 Pre Clean with Sampl
83. s how to install the Cycle Composer Macros or ICC Cycles how to operate the DLW and a step by step illustration of the DLW cycles Contents e Overview e Installing Cycle Composer Macros or ICC Cycles e Operating Dynamic Load and Wash DLW e DLW Cycle Step by Step Overview This section contains an overview of the Dynamic Load and Wash DLW option Figure 34 Dynamic Load and Wash DLW Thermo Scientific Accela Open Autosampler User Guide 63 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles The DLW option represents a new wash station concept that combines an injection cycle with wash steps The linked combination of the two steps which are usually separate minimizes cycle time and carryover There are two characterizing features for the DLW option e The sample solution never contacts the syringe itself it is held between the holding loop e Wash solvents are pumped from back to front into the DLW system to intensely flush all critical parts that are in contact with the sample The DLW option consists of two self priming micro pumps mounted on a dedicated bracket and the wetted parts are Ryton PPS and Kalrez FFPM The pump IN ports are connected to the wash solvent bottles and the OUT ports are connected to the DLW manifold which is part of the assembly of the dedicated DLW syringe holder A holding loop separates the syringe from the DLW actuator to prevent
84. s list select the tray location in the three drawer deep drawer configuration tray 1 amp 2 drawer 1 tray 3 amp 4 drawer 2 tray 5 amp 6 drawer 3 Up to six drawers and twelve trays are possible with the shallow drawer configuration Type From this list select the tray type Tray Holder Stack 1 and Stack 2 are the allowed values Tray holders can hold one or more trays A Stack is a tray holder that is designed to hold microplates The autosampler firmware determines the type and number Vials in X Direction Vials in Y Determined by choice of tray type Direction Specifying the Location of the Direct Control Methods Use the Direct Control Configuration dialog box to specify the location of the methods that you can open in the Methods page of the Direct Control dialog box To specify the method folders 1 Open the Direct Control dialog box see Opening the Direct Control Dialog Box on page 53 2 Click the Direct Control Configuration tab The Direct Control Configuration page appears see Figure 33 By default the methods that appear in the Method list on the Method page are located in the following directory drive Xcalibur Methods Thermo Scientific Accela Open Autosampler User Guide 59 4 Using the Direct Controls to Operate the Autosampler Specifying the Location of the Direct Control Methods Figure 33 Direct Control Configuration page Direct Control Methods Tray Configuration Dire
85. sample from contacting these parts The syringe and holding loop are preloaded with wash solvent 1 at the start The sample is picked up and remains separated from wash solvent 1 by an air gap After loading the loop and injection wash solvent 1 is pushed into the system followed directly by wash solvent 2 to flush the critical valve paths The DLW syringe assembly is moved to the wash station for further cleaning steps and for preparing the syringe and holding loop for the next cycle For further details see DLW Cycle Step by Step on page 75 Installing Cycle Composer Macros or ICC Cycles You can only operate the autosampler DLW option with PAL control software such as the Thermo PAL driver for the Xcalibur data system or Cycle Editor for ICC interpretation in another data handling system software For software control three macros or three cycles are provided The first of three macros covers the initial and daily priming of the solvent lines and covers a complete injection cycle a second macro is used for Standard speed with optimized washing possibilities and the last for a Fast cycle for optimized throughput and less focus on carryover See Table 13 For detailed descriptions of all macros or cycles see Table 13 Figure 14 on page 66 and Figure 15 on page 68 64 Accela Open Autosampler User Guide Thermo Scientific 5 Using Dynamic Load and Wash DLW Installing Cycle Composer Macros or ICC Cycles Table 13
86. strument Method Parameters for the Autosampler Figure 17 Method Setup page for the autosampler Method Setup Method Summary Template Selection Template Method Folder C Xcalibur System PAL Methods Browse S Template Description Macro Variables Browse button Syringe Macro Sequence Recommended Injection Volume Volume fpl Look Ahead Injections Delay Time mins Help 4 Under Template Selection click Browse and browse to the folder that contains the autosampler methods pme For a standard installation the folder that contains the autosampler method is located in the following directory see Figure 18 drive Thermo Instruments LC Devices AccelaOpenAS PAL methods 26 Accela Open Autosampler User Guide Thermo Scientific Thermo Scientific 2 Getting Started Specifying the Instrument Method Parameters for the Autosampler Figure 18 Location of Accela Open Autosampler template amp C Thermo nstruments C Devices AccelaOpenAS PAL methods File Edit View Favorites Tools Help ay Q sax Q i JO Search IE Foes E Address C Thermo Instruments LC Devices AccelaOpenAs PAL methods v gt Go Folders x Name Size Type Date Modified Desktop A DLW Priming_RevO1 PME 2KB PMEFile 3 31 2010 9 28 AM aw B My Documents Fast Injection Accela Open pme 2KB PMEFie 3 31 2010 9 28 4M my Computer 3 LC Inj DLW Fast_Rev03 PME 2KB PMEFile 3 31 2010 9 28 AM E GB 3 Floppy a
87. the 98 x 2 mL tray holder Table 2 lists the contact closure cables used to connect an Agilent pump to a Thermo Scientific MS detector and an autosampler You can order the DB15 x 15 wire cable from Agilent or Thermo Fisher Scientific Table 2 Contact closure cables used to connect an Agilent pump Part number Supplier Description CBL 7890 CTC Analytics PAL Interface Cable for APG Remote DB15 connector X DB9 connector cable 00012 27716 Thermo Fisher Scientific DB15 connector x 15 wire cable G1103 61611 Agilent DB15 connector X 15 wire cable Setting Up an LC MS System with the Autosampler Typical stackable setups include placing the autosampler on top of the two pumps in a dual pump setup and to the left of the MS detector Ensure that the stackable area for the autosampler is level and that system cables behind the autosampler have adequate space CAUTION To prevent damage to the injection unit during operation place the autosampler on a level surface For information on assembling the Accela Open Autosampler and connecting the solvent lines refer to the Accela Open Autosampler Hardware Manual supplied in hard copy format with the autosampler During the installation of a Thermo Scientific MS detector a Thermo Fisher Scientific field service engineer connects the LC system to the MS detector 2 Accela Open Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Figure 1 shows a
88. to review 3 Click the Method Summary tab The Method Summary page appears with a summary of the autosampler parameters for the active instrument method see Figure 20 Thermo Scientific Accela Open Autosampler User Guide 33 2 Getting Started Viewing the Method Summary Figure 20 Method Summary page Description Duplication of PAL local LC Inj cycle This is only a sample method for short runs For analytical work the method paramet ers must be set according to the specific needs Syringe 100ul Macro Sequence LC Inj Step 1 of 1 Air Volume pl 0 Pre Clean with Solvent 1 0 Pre Clean with Solvent 2 0 Pre Clean with Sample 0 Filling Speed pls 50 Filling Strokes 1 Inject to LC Vlv1 Injection Speed pl s 100 Pre Inject Delay ms 500 Post Inject Delay ms 500 Post Clean with Solvent 1 1 Post Clean with Solvent 2 0 Valve Clean with Solvent 1 1 Valve Clean with Solvent 2 1 Recommended Injection Yolume Volume 10 00 Injection Look Ahead Delay Time 0 00 Macros used within method Macro name LC Inj COMPLETE CYCLE XXXXXXXXXXXXXXXXXXXXXXXX Duplication of PAL s local LC injection cycle Tray Name Sample Number and Sample Volume are taken from the sample list Variables 14 Help Method Summary 34 Use the Method Summary page to view the details of the Macro Sequence that makes up an autosampler Method or Template The contents of the Method Summary Template Summar
89. tosampler Interconnect cable to Accela Pump adapter cable connection Goc55g000 Qe T0 Msa iTo Lxa fro 60157 63024 hw 60157 63022 J Accela Open Autosampler Accela Pump interconnect cable adapter cable 3 Connect the 8 pin connector of the Accela Pump adapter cable to pins 1 through 8 on the back panel of the Accela Pump see Figure 10 on page 13 Thermo Scientific Accela Open Autosampler User Guide 11 1 Getting Connected System Synchronization Connections 4 Connect the 2 pin connector of the Accela Open Autosampler interconnect cable to the mass spectrometer see Connecting the Accela Open Autosampler Interconnect Cable on page 5 12 Accela Open Autosampler User Guide Thermo Scientific 1 Getting Connected System Synchronization Connections Figure 10 Accela Open Autosampler interconnect cable and Accela Pump adapter cable connections ay va N a I INTERFACE 1 Peripheral Control Accela Open St Reay
90. y page is read only Accela Open Autosampler User Guide Thermo Scientific ee i Creating Custom Templates and Macros This chapter describes how to create custom templates and macros Contents e Using the Template Editor to Create Custom Templates e Testing a Custom Template e Viewing the Template Summary e Standard Macros e Using the Macro Editor to Create Custom Macros e Defining Variables Using the Template Editor to Create Custom Templates Three standard templates are provided with the autosampler To create a custom template use the Template Editor dialog box Creating a Custom Template This section provides information about how to create a custom template To create a custom template 1 Open the Template Editor page as follows a Open the Instrument Setup view for the autosampler see Specifying the Instrument Method Parameters for the Autosampler on page 24 b From the menu bar choose Accela Open AS gt Template Macro Editor The Template Editor page of the Pal Template Macro Editor window appears see Figure 21 Thermo Scientific Accela Open Autosampler User Guide 35 3 Creating Custom Templates and Macros Using the Template Editor to Create Custom Templates Figure 21 Template Editor page of the Pal Template Macro Editor window Pal Template Macro Editor File PAL View Help rh amp x 2 Template Editor Macro Editor Template Summary Template Description M

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