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1. Gat oF Genemed PAP Pen Cat No 10 0041 10 0045 Intended Use Reagents Supplied Summary And Explanation Recommended Usage Storage Precautions Symbols 30369 Rev 01 Genemed Biotechnologies Inc east 458 Carlton Ct South San Francisco CA 94080 U S A Tel 650 952 0110 Fax 650 952 1060 Biotechnologies Inc Product Mini PAP Pen Super PAP Pen For In Vitro Diagnostic Use This product is intended to be used for immunohistochemical IHC procedures The hydrophobic properties of PAP Pen allow the user to create a barrier on the slides in any pattern to confine the flow of reagents to a defined area One Mini PAP Pen or one Super PAP Pen During IHC staining it is necessary to wipe off the liquid around the sections to reduce dilution of the next reagent applied on the specimen This is a time consuming and tedious process This drawback can be avoided when using PAP pen After drying the circle drawn with the PAP Pen is water repellent and alcohol and acetone insoluble The circle provides a barrier to liquids such as antibody enzyme and chromogen solution applied to the sections Using PAP Pen can reduce turn around time for staining by eliminating the need for wiping off liquid around sections between different steps of the staining procedure It reduces the volume of required reagent and it also provides more uniform staining results The PAP Pen is well suited for use on most typ
2. es of coated and uncoated microscope slides Before first time use or when the pen tip is dry 1 Press tip down against blank glass slide or paper towel to gradually moisten the felt tip with pen s liquid Note Repeat this step when pen starts to dry out After de paraffinization and rehydration of tissue sections 1 Draw a circle around tissue section with the PAP Pen 2 Let circle dry 1 2 minutes at room temperature Be sure not to let tissue sections dry out 3 Continue with normal IHC process Note De paraffinization and heat induced epitope retrieval HIER should be performed before use of PAP Pen since xylene dissolves the drawn circle and very high temperatures tend to destroy the hydrophobic properties of the circle Store at room temperature with cap on tight and tip pointed down All performance claims are void after the expiration date For professional users only Risk Statements R10 Flammable R36 37 38 Irritating to eyes respiratory system and skin R48 20 Harmful danger of serious damage to health by prolonged exposure through inhalation R63 Possible risk of harm to the unborn child 25 C REF LOT IVD X ba IVD en Catalog No Batch No In Vitro Diagnostic Use Temperature Range Use By Page 1 of 1 MDSS GmbH Schiffgraben 41 C 30175 Hannover Germany

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