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Thermo Scientific Multiskan GO

1. Contact service 37 Lamp or AD converter gain setting error Please restart the instrument Contact service 38 Grating home position is too far from the 0 nm reflection angle Contact service 39 Wavelength calibration is not done Contact service 40 No cuvette blank Please measure blank first Select cuvette zero from the user interface before measurement 41 Detector operation failure Please restart the instrument Contact service 42 Cuvette hardware not detected Contact service 43 Absorbance value below measurement range minimum Please check that cuvette zero has been correctly performed Please retry the operation Ensure that the sample you are measuring in the cuvette station has equal or higher absorbance than the blank you used for blanking the cuvette station Use correct cuvette measurement orientation Contact service 44 Measure head X and plate Y axis are not perpendicular Contact service Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 73 Troubleshooting Guide USB memory device 45 The instrument serial number does not match with Contact service the detected instrument configuration 46 Did not find 261 nm calibration peak Contact service USB memory device Contacting service 74 Thermo Scientific Multiskan GO User Manual It is recommended
2. Cuvette Yes Reset to factory defaults The Settings configuration window contains the following parameters e Language Select the user interface language English Chinese French German Japanese Portuguese Russian or Spanish To change the user interface language refer to Changing the language on page 22 e Power Save Select the Power Save setting No 5 min 15 min 30 min or 60 min Thermo Fisher Scientific Instrument User Interface Settings menu For more information on Power Save refer to Power Save feature on page 24 e Export decimal delimiter Select the decimal separator Dot or Comma This setting defines the decimal delimiter only for exported measurement results It does not affect the displayed or printed results which always use the decimal point e Reset to factory defaults Reset the instrument to its factory default values Date amp Time In the Date amp Time parameters submenu you can set the instrument date and time and the date and time formats You should set the correct time before you put the device into use Once the time is set the instrument clock will keep running even when the instrument power is switched off When you connect the Multiskan GO to SkanIt Software the instrument date and time is set automatically 4 Settings Date amp Time parameters 14 04 2010 12 28 30 Date format dd mm yyyy Time format 24 hour Cancel
3. 00 00 00 Shaking No ee Required temp No Measurement 450 nm 620 nm F 24 2200 Cancel The dimensions of the plates when using the instrument user interface are given in Table 5 4 and Table 5 5 Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 35 Instrument User Interface Plate measurement Measurement area 36 Thermo Scientific Multiskan GO User Manual Table 5 4 96 well plate dimensions 96 well plate Dimension mm Plate length Plate width 85 48 127 76 14 38 First well position X First well location Y Corner distance X Corner distance Y Table 5 5 384 well plate dimensions 384 well plate 11 24 99 63 Dimension mm 85 48 Plate length Plate width First well position X First well location Y Corner distance X Corner distance Y 121 16 12 13 8 99 103 5 67 5 The maximum plate height with lid is 18 5 mm If you wish to measure only a certain part of the microplate you can define a measurement area To select a single rectangular measurement area 1 Select the starting point with the arrow keys and press the OK key The OK key toggles between cursor movement and area selection The first point can be any point on the layout 2 Select the last point by highlighting the desired rectangular measurement area on the plate with the arrow keys Thermo Fisher Scientific Instrument User Interface Plate measurement Selected area c3 F7
4. Fasult Highlight a menu item using the UP and DOWN arrow keys and select the item by pressing the OK key Selecting an item opens a selection box or enters a submenu in which you can make selections using the arrow keys and the OK key Settings Date amp Time parameters Time 14 04 2010 12 27 59 Date format Time format Cancel In number or text fields you can enter characters with the UP and DOWN arrow keys or the alphanumeric keys Settings Date amp Time parameters Time 14 04 2010 12 26 30 Date format dd mm yyyy Time format 24 hour Cancel Thermo Scientific Multiskan GO User Manual 31 Instrument User Interface Instrument incubator In each view of the user interface the info text bar shows the actions available for the F1 F3 keys Bb Ae 3000 Results The info text bar also displays the current instrument temperature as well as the target temperature when the incubator is activated If a printer or USB memory device is connected to the instrument a corresponding icon is displayed on the info text bar Note The Multiskan GO maintains the used settings and parameters I until these are changed A Instrument The incubator temperature can be controlled from the Plate and Cuvette menus using the Fl key incubator The info text bar shows the current temperature in degrees Celsius C Depending on the selected menu either the plate measurement chamber or the cuvette
5. Intended users How to use this user manual Thermo Scientific Skanlt Software Thermo Fisher Scientific About This User Manual Intended users About This User Manual The Multiskan GO as standalone or with Thermo Scientific SkanIt Software can be used in research laboratories by professional personnel This user manual is for the following instruments Multiskan GO w o cuvette Cat no 51119200 and 51119250 Japan and Multiskan GO with cuvette 51119300 and 51119350 Japan It aims to give you the information you need for e Reviewing safety precautions e Installing the Multiskan GO e Navigating in the Multiskan GO user interface e Operating the instrument e Programming measurement sessions e Performing basic cleaning and maintenance procedures e Troubleshooting the instrument This user manual also describes all the features and specifications of the Multiskan GO instrument as well as ordering information Note that simulated data appears in the screen captures Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available For SkanIt Software related issues refer to the Skan t Software for Microplate Readers User Manual Cat No N16243 or Skanlt Software for Microplate Readers Technical Manual Cat No N16046 The SkanIt Software for Microplate Readers Research Edition CD
6. The Date amp Time parameters submenu contains the following parameters e Time Set the instrument date and time e Date format Select the date format dd mm yyyy dd mm yyyy dd mm yyyy yyyy mm dd or mm dd yyyy e Time format Select the 12 hour or 24 hour time format Print header You can also include the print header in the printed report and export file if it has been defined in the settings Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 53 Instrument User Interface Export file formats Status report Export file formats 54 Thermo Scientific Multiskan GO User Manual Settings Print header Enable header Yes Header text Lab info The Print header submenu contains the following parameters e Enable header Enable or disable the print header If activated the header is used for all printouts and is displayed at the top of each page e Header text Enter a header text A customized print header can contain for example laboratory information The maximum length of the header is approximately 50 characters You can view export and print the instrument s status report Settings 1009 INSTRUMENT STATUS REPORT 03 06 2010 10 09 18 Instrument Name Multiskan GO Cuvette Yes Serial number 1510 00000C FW version 1 00 24 Startup tests Plate movement NOT TESTED Measure head movement PASSED amp 2200 The status report provides current information on
7. Thermo Fisher Scientific Warning and other markings used in the documentation Thermo Fisher Scientific DPP 5 About This User Manual Safety symbols and markings A black label with the following text Figure 2 3 CAUTION WARNING DISCONNECT SUPPLY BEFORE SERVICING and AVERTISSEMENT COUPER L ALIMENTATION AVANT L ENTRETIEN ET LE DEPANNAGE 4 The following symbols and markings appear in this user manual Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A Thermo Scientific Multiskan GO User Manual 5 About This User Manual Safety symbols and markings 6 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents rr A ti a oe ae ae ee Uae 3 How to Wise this User Miata bea EN RN 3 Thermo Scientific Skanlt Software ooooooo 3 For more INFOMATION akan NN 4 Saten symbols and marking ae ia AI 4 Introduction to the Multiskan GO ooooooooo oo oom 9 Intended use an en ty Sse State ee ee a 10 General Description iii ee 11 ASEEUMENE VIC WS tic scott dde 11 Pe ae a aetna 12 Cu CEES Dorna anta ahem
8. 3 Start End wavelength 200 1000 nm L Step 1 800 nm L Mode Fast Precision 34 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Plate measurement Session name The Session row in the Plate menu shows the name of the active session Sessionl is the default active session until the first Save As operation A session name can be up to 15 characters long You can open a session by pressing the OK key on the Session row or by pressing the FILE key and selecting Open from the FILE menu The list of saved sessions shows the session names and the date and time of the last save You can scroll the session list with the UP and DOWN arrow keys You can store at least one hundred 100 single wavelength 96 well measurement sessions in the instrument s memory There are two types of sessions those that have not been run sessions without measurement data and those that have been run sessions containing measurement data Note Session contains data of one measurement only A Press the F3 Sort key to sort the list of saved sessions by their name or by the date they were last saved e Save Overwrites previous results from the same session e Save As Creates a new session file including the results Plate format You can select the utilized plate format 96 or 384 wells with the Plate format setting Session Session12 Plate format 96 wells 364 wells Area All
9. 8 Troubleshooting Guide The external printer must support HP PCL 5 PCL5e or PCL5c emulation types Figure 2 3 on page 11 Typically printers with emulation type PCL6 also support PCL5 You can print measurement data with or without printout header texts Skanlt Softwa re The instrument can be controlled with SkanIt Software for Microplate Readers which controls all the instrument functions and provides data processing as well as reporting functions For more information refer to the Skanlt Software for Microplate Readers User Manual Cat no N16243 or SkanIt Software tor Microplate Readers Technical Manual Cat No N16046 Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 15 General Description Skanlt Software 16 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific What to do upon delivery Unpacking the Instrument a Checking delivery for completeness and damage Environmental Thermo Fisher Scientific requirements Chapter 3 Installation This chapter describes the installation of the Multiskan GO instrument This section covers the relevant procedures to be carried out on receipt of the instrument Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective plastic wrapping until the ambient temperature has been reached Unpack the Multiskan GO instrument and its accessories carefully so t
10. Cat no 5187139 includes the following manuals e SkanIt Software tor Microplate Readers User Manual and its respective language versions eo SkanIt Software tor Microplate Readers Technical Manual Thermo Scientific Multiskan GO User Manual 3 About This User Manual For more information For more information Safety symbols and markings Safety symbols and markings used on the Multiskan GO Bc jo 4 Thermo Scientific Multiskan GO User Manual For the latest information on products and services visit our website http www thermoscientific com http www thermosci entific com mpi http www thermoscientific com platereaders http www unitylabservices com In our efforts to provide useful and appropriate documentation we would appreciate any comments you may have on this user manual to your local Thermo Fisher Scientific representative Manufacturer Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 FI 01621 FINLAND These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards The following symbols and markings appear on the type label and the instrument itself Power on A Power off a Serial number a Catalog number a Date of manufacture A Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC 4
11. TAT CUYOOO0S TAT CUYOOO02 TAT CUYOOO01LTAT 10 04 2010 16 59 06 10 04 2010 15 20 36 10 04 2010 15 26 38 10 04 2010 15 25 45 10 04 2010 15 23 34 05 04 2010 15 26 46 Printing results Settings menu Thermo Fisher Scientific Instrument User Interface Settings menu e Append the current cuvette session to the end of the selected session on the USB memory device by pressing File gt Export append Press the UP and DOWN arrow keys and the F1 Accept or the OK key to select the session Nucleotide dsDNA lt A260 A320 gt lt A280 4320 gt 120 50 4260 6 83 pg ml You can print measurement results directly to a connected USB printer In addition to session and run information the printed report also includes the print header if it has been defined in the settings The Settings menu allows you to configure instrument operation The settings are universal for the instrument and do not differ between plate and cuvette use The values shown in the Settings menu are stored in the instrument memory and are not session specific Settings Configuration Date amp Time Print header Status report The Settings menu contains the following parameters e Configuration Select the user interface language Power Save setting and export decimal delimiter or reset the instrument to factory default values e Date amp Time Set the instrument date and time and select the date and time form
12. a Enter the value for constant a The available range is 0 0001 1 000 000 e Wavelength 1 3 Enter the measurement wavelength The available range is 200 1000 nm e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength e Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Thermo Scientific Multiskan GO User Manual 47 Instrument User Interface Cuvette measurement Kinetic Use the Kinetic session to perform a kinetic measurement on a cuvette Cuvette Kinetic parameters Wavelength nm 1 Pathlength correction No Number of readings 100 Interval mm ss 00 00 Total time 0 00 16 Cancel The Kinetic submenu contains the following parameters e Wavelength Enter the measurement wavelength The available range is 200 1000 nm e Number of readings Enter the number of readings The range is 1 100 e Interval If the Number of readings is greater than 1 the measurement is considered kinetic and you have to set the time interval 00 00 05 00 When selecting 00 00 the instrument measures as fast as possible The total kinetic measurement time is calculated and displayed e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 1
13. chamber door e Use the original packaging for shipping e Pack the instrument according to the packing instructions e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system can be very useful in properly maintaining the system Refer to Appendix A System Log Copy the table as many times as necessary but leave the blank original inside the user manual If the Multiskan GO is exposed to potentially infectious chemical samples toxic or corrosive chemicals or radioactive chemicals waste management of the complete instrument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 59 A Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Warning A used lithium Li battery is regulated waste and must be disposed of ac
14. is selected by entering 0 zero In a Spectrum measurement you define the start and end wavelengths for a spectral scan The end wavelength must be greater than the start wavelength The start wavelength range is 200 999 nm and the end wavelength range is 201 1000 nm Moasurement parameters Moasurement parameters Measurement parameters Wavelength mm 450 Wavelength 1 nm 450 Start wavelength nm 200 Wavelength 2 mm bal End wavelength nm 1000 Wavelength 3 mm oper Step nm 2 Aobept anaal E PC Canael Cancel single Multiple spectrum e Step Only used in Spectrum measurements The measurement step range is 1 800 nm in increments of 1 nm e Mode Select the measurement mode Fast or Precision The default Fast mode is a measurement speed optimized mode that produces results that are sufficiently precise for most uses The Precision mode takes more time to perform a measurement when the best possible measurement precision is reguired Plate results After the instrument has completed a measurement it automatically displays the results In single and multiple wavelength measurements the results are displayed as a table while spectral scanning measurements are displayed as a graph Cuvette Session12 Spectrum a o N o y a sa 20 O oF O NN 2 o o 90 ae o oo N UG OU D uu N oO an o oF a oF 5 o a N a al N N N 2 Ap gt ya Qu Gal Nu Nh 0 OF O
15. nm F Results The asterisk by the session name indicates unsaved results These are retained until new results are generated To store results for later use you should save them in the instrument s memory export them to a connected USB memory device or print them on a connected USB printer Saving results To save results in the measurement results view select the F2 Save As key Or press the FILE key to open the File menu and select either the Save or Save As menu item e Save Overwrites previous results from the same session Session contains only data for one measurement e Save As Creates a new session file with the results data for one measurement Abs 2 00 1 50 1 00 2000000000000 ia i i 942 ee YAM cc MAN 88 as 200 300 400 500 600 700 800 900 1000 902 1733 nm Cancel Exporting results 40 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Printing results Cuvette measurement Thermo Fisher Scientific Instrument User Interface Cuvette measurement You can export measurement results to a connected USB memory device Figure 5 15 by pressing the F1 Export key in the result view or use the File menu Selecting Export in the Results view automatically exports the current session By using Export in the File menu you can export multiple sessions simultaneously You can also import plate measurement results into SkanIt Software For more information re
16. not optimal and V bottom plates are not recommended The plate carrier Figure 2 4 acts as the sample holder The maximum plate height of a combined plate and lid is 18 5 mm Cuvette port The Multiskan GO with cuvette Figure 2 4 has an incorporated cuvette port that can measure the absorbance of solutions in suitable cuvettes Cuvette port dimensions for selecting suitable cuvettes are presented in Table 2 1 Table 2 1 Cuvette port properties Cuvette width x depth Cuvette height Measurement beam Beam diameter position 12 5 x 12 5 mm 40 58 mm 8 5mm lt 1 8 mm Beam center height from the bottom of the cuvette port Cuvettes corresponding to the dimensions in the table above can be used with the Multiskan GO cuvette port Refer to Appendix C Example of Available Cuvettes 12 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific General Description Optical system To maximize the linear range the following cuvette window sizes must be used with the Multiskan GO e When plastic cuvettes are used in the UV area the window can be 2 mm or more e When plastic cuvettes are used in the VIS area the window must be at least 3 mm e When masked wall cuvettes are used the window can be 2 mm or more e When clear quartz or glass cuvettes are used the window must be at least 3 mm Black masked wall cuvettes are always recommended with the Multiskan GO over clear quartz or glass cuvettes beca
17. outside of the instrument or to remove stains using a cloth dampened with 70 ethanol Place the instrument in a large plastic bag Ensure that the cuvette port and the measurement chamber door are open and the plate Carrier is out Place a cloth soaked in the prepared decontaminant solution into the bag Ensure that the cloth does not come into contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag After decontamination clean the instrument using a mild detergent After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package Thermo Fisher Scientific Refitting the transport lock Thermo Fisher Scientific Maintenance Refitting the transport lock When you relocate the instrument or ship it for service make sure that y p you refit the transport lock Figure 6 16 Figure 6 16 Transport lock bar with warning tag To refit the transport lock 1 Switch the instrument off by pressing and holding the STOP OFF key for a few seconds This drives the measurement head and plate carrier into the correct positions 2 Disconnect the power supply and USB communication cable and any connected peripheral devices 3 Open the measurement chamber door by pushing it from the bottom edge and pulling it from the top ed
18. station temperature is displayed Press the F1 key to set the instrument incubator temperature Cuvette Settings Cancel The temperature setting range is 10 45 C with a resolution of 1 C When the heating is on and the temperature set the target temperature is displayed on the info text bar see below 32 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Instrument incubator Cuvette 1 Settings Single Spectrum DNA RNA You can toggle the instrument incubator on and off with the Fl key When you turn off the heating the heating icon blinks for a few seconds Note The Plate incubation setting Required temp does not affect the I real instrument temperature but is only used as a session specific temperature reminder for the user A File menu The File menu provides you with data management functions such as exporting and printing results or storing session data File Press the FILE key to open the File menu in the Plate or Cuvette menu Use the arrow keys to highlight the desired menu function and press OK to select it In the Plate menu you can open save and delete sessions from instrument memory as well as export and print measurement results In the Cuvette menu you can export and print results but not save session parameters or results in the internal memory Instrument help The instrument Help feature presents information on the menu items in
19. the instrument status including the instrument model and serial number firmware version current temperature and other parameters When exporting data to an external USB memory device the Multiskan GO stores the files in a folder named GO The folder is created automatically if it does not already exist The Multiskan GO can export three types of files e Plate measurement results PLA TXT e Cuvette measurement results CUV TXT e Instrument status reports REP TXT All files of the same type are numbered sequentially PLA00001 TXT PLA00002 TXT and so on Thermo Fisher Scientific Thermo Fisher Scientific Instrument User Interface Export file formats The exported files are in ASCII format and can be opened with any text editor such as Microsoft Notepad Result files can be imported into Microsoft Excel The measurement results are exported using four decimal places Note Set the export decimal delimiter to the desired value in Settings gt Configuration A Plate sessions saved in the instrument memory can be transferred to SkanIt Software Cuvette data cannot be transferred to SkanIt Software The checksum at the end of exported files is used by SkanIt Software when importing data Plate and cuvette export files contain the following information e Print header e Measurement session information e Instrument information e Session start and stop times e Temperatures at the beginning and end of th
20. through the cuvette This leads to incorrect zero levels A To zero the cuvette station press the F2 Zero key The yellow triangle alerts the user when the zero is not valid Cuvette 44 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Cuvette measurement When Zero is pressed the instrument measures the current absorbance level and sets it to 0 000 Abs The following applies for zeroing e Itis not possible to start a cuvette measurement without a valid zero value e The user interface shows the status of the zeroing e There are separate zero values for each measurement type The zeroing is valid only if it has been performed with the same parameters as during the session e The zeroing values are valid for ten hours or until the measurement mode or parameters are changed e The zeroing values are reset at shutdown Single wavelength Use the Single session to perform a single wavelength measurement on a cuvette Cuvette Single parameters Wavelength nm Pathlength correction Pathlength mm Cancel The Single submenu contains the following parameters e Wavelength Enter the measurement wavelength The available range is 200 1000 nm e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength e Pathlength Enter the cuvette pathlength The avail
21. 0 48 00 45 00 45 00 45 00 45 00 45 00 45 00 Width mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Example of Available Cuvettes Depth mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Volume pl 800 400 3500 2500 2500 3500 1400 55 65 115 Ia 700 Meas position height mm 4 40 2 40 4 40 4 43 4 43 4 43 8 5 8 5 8 5 8 5 8 5 4 40 Thermo Scientific Multiskan GO User Manual 83 Example of Available Cuvettes Manufacturer Brand http www brandtech com Kartell http www kartell it uk labware w dis po htm Sarstedt http www sarstedt com php main php GE Healthcare http www gehealthcare com worldwide html 84 Thermo Scientific Multiskan GO User Manual Table 9 15 Disposable plastic cuvettes Code s 759070D 759071D 759080D 759081D 199170 759075D 759076D 759085D 759086D 159165 799150 759152 759210 759200 759215 759218 1937 1938 67 742 67 746 67 740 67 741 67 745 67 738 80 2004 53 80 2084 11 80 3000 77 Pathlength mm 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 Height mm 45 00 45 00 45 00 45 00 45 00 45 00 45 00 45 00 45 00 45 00 Width mm 12 50 12 50 12 50 12 50 12 50 12 50 1
22. 0 mm pathlength e Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Cuvette results The results of a cuvette measurement are displayed automatically after the measurement Note The user cannot save cuvette sessions or measurement results to the instrument s memory The last result of all predefined cuvette sessions is automatically saved until parameters are changed or a new cuvette measurement is run If you want to save the cuvette results transfer them immediately to a USB memory device after the measurement 48 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Instrument User Interface Cuvette measurement The presentation of the results depends on the session Single wavelength sessions the latest result is presented at the top and the measurement history is listed below it in three columns An entry of 0 0000 Z indicates instrument zeroing Cuvette Single Latest 0 3107 The measurement time is displayed to the right of each result Up to 24 measurement results are simultaneously displayed on the screen However the instrument stores up to 100 latest measurements which you can retrieve by exporting the single measurement to the USB memory device or by printing the results Press the C key to clear the result history list Spectrum sessions the measured spectrum is di
23. 10 20 10 00 45 00 12 50 1250 3500 2 40 100 10 40 100 10 46 6030 10 10 6030 UV 10 531 110 10 20 10 00 46 00 1250 1250 3500 2 40 110 10 40 110 10 46 6040 10 10 10 00 45 00 12 50 12 50 1400 4 40 6040 UV 10 531 104 10 20 104 10 40 104 10 46 104B 10 20 104B 10 40 104F 10 40 114 10 20 10 00 46 00 12 50 12 50 1400 4 40 114 10 40 114B 10 40 104 002 10 20 10 00 45 00 12 50 12 50 700 4 40 104 002 10 40 104 002B 10 20 104 002B 10 40 104 002F 10 40 115 10 40 10 00 40 00 12 50 12 50 400 2399 1158 10 40 105 200 85 40 10 00 45 00 12 50 12 50 180 8 5 105 201 85 40 10 00 45 00 12 50 12 50 120 8 5 105 202 85 40 10 00 45 00 1250 12 50 70 8 5 105 203 1085 40 Thermo Scientific Multiskan GO User Manual 81 Example of Available Cuvettes Manufacturer Code s Pathlength Height Width Depth Volume Meas mm mm mm mm pl position height mm Hellma Cont 105 204 1085 40 10 00 45 00 250 1200 40 8 5 105210 585 40 5 00 40 00 12907 1250 D 8 5 105210 1085 40 10 00 40 00 12 50 12 50 10 8 5 107 100 10 40 10 00 56 00 12 50 1250 3500 2 50 107 104 10 40 10 00 56 00 12 50 12 50 1400 2 50 Spectrocell R 0010 10 00 45 00 12 50 12 50 3500 2 40 R 2010 R 3010 R 4010 R 2010 T 10 00 55 50 12 50 12 50 3500 2 38 R 3010 T R 4010 T R 0010 S 10 00 57 00 12 50 12 50 3500 2 40 R 2010 S R 3010 S R 4010 S SM 0010 10 00 45 00 12 50 12 50 1400 4 40 SM 2010 SM 3010 SM 4010 SM 3010 B SM 4010 B
24. 2 50 12 50 12 50 12 50 Depth mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Volume pl 4500 3000 850 4500 1500 1500 4500 4500 2500 800 Meas position height mm 4 40 4 20 8 5 4 40 4 40 4 40 4 40 NA NA NA Thermo Fisher Scientific Glossary absorbance optical density A logarithmic function of the transmission of a wavelength of light through a liquid log 4 dimension Abs decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination disinfection The destruction of pathogenic bacteria usually with an antiseptic chemical or disinfectant dynamic range The range of signals an instrument can read from the minimum to the maximum detectable For example a dynamic range of three decades means that the difference between the lowest and highest signals that can be measured is 10 EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions ELISA Abbreviation for enzyme linked immunosorbent assay kinetic measurement Measurement over a time period at certain intervals memory device A USB memory stick to store results for later reference The results are saved in the instrument s memory and exported to the connected
25. 37 incubator 14 32 33 41 66 info text bar 30 32 installation 3 17 18 24 25 67 68 category 67 68 intended use 3 10 internal memory 33 Thermo Scientific Multiskan GO User Manual 87 K keypad 24 29 56 65 keys 21 22 23 24 25 26 27 30 31 32 33 35 36 37 39 40 43 48 49 50 60 kinetic 9 41 42 47 49 84 measurements 9 47 49 84 session 47 49 L lamp 24 65 layout 36 linear shaking 65 low volume plates 12 maintaining a system log 55 62 maintenance 3 14 55 56 62 checklist 55 measurement 3 12 13 14 15 18 19 21 26 27 30 31 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 52 54 57 59 60 61 62 65 66 68 84 chamber 18 19 21 57 59 60 61 62 chamber door 18 19 21 59 60 61 62 direction 13 26 mode 39 44 45 parameters 26 31 34 38 41 speed 39 45 66 technique 66 time 48 measuring 25 26 84 a cuvette 26 a plate 25 memory device 15 65 84 menu 22 30 31 32 33 35 40 42 navigating 3 29 0 optical 13 14 43 55 56 84 system 13 14 55 56 ordering information 3 74 p packing 17 62 63 88 Thermo Scientific Multiskan GO User Manual instructions 62 list 17 materials 63 photometric 10 assay 10 photometry 66 84 plate 9 10 12 14 15 19 21 25 26 27 30 31 32 33 34 35 36 37 39 40 42 50 53 54 55 57 58 59 60 61 62 65 66 8
26. 4 measurement 14 32 34 40 53 menu 26 33 34 35 results 39 type 26 Power Save mode 9 24 50 51 52 65 84 precision 34 39 42 45 66 printer 15 20 32 40 41 50 65 printing 33 41 48 50 results 33 41 50 printing or exporting data 26 27 41 refitting the transport lock 60 62 remote control 24 30 S saving results 40 self diagnostics 21 25 84 service 17 21 55 56 58 60 62 69 74 Settings 22 23 24 31 50 51 54 menu 22 23 24 31 50 51 shaker 15 65 shaking 9 15 34 37 65 speed 15 37 shutdown 44 Single 34 37 38 39 41 42 44 48 single measurement 38 48 84 spare parts 74 specifications 3 65 general 65 performance 66 safety 63 67 Spectrum 34 38 39 41 42 44 45 48 speed 15 34 37 66 startup 25 54 switching on 25 symbols 4 Thermo Fisher Scientific system 2 5 10 62 76 84 configuration 51 log 62 T transport 17 18 19 20 59 60 61 62 65 74 package 17 59 troubleshooting 3 15 69 type label 4 56 U USB 15 20 32 40 41 47 48 49 50 53 60 65 74 84 memory device 15 32 40 41 47 48 49 50 53 84 User formula 41 42 46 49 user interface 3 22 24 30 31 32 35 44 50 51 65 Thermo Fisher Scientific V viewing results 26 27 W warning codes 70 warranty 17 wavelength 9 34 35 38 39 42 44 45 46 47 48 65 66 84 accuracy 66 range 9 38 66 r
27. Chapter 5 Instrument User Interface This chapter describes the display and the keypad Figure 5 14 for navigating the menus and editing the parameters of the Multiskan GO instrument MULTISKAN GO Session Plate format Area Incubation Measurement Menu Session12 96 wells All 00 00 00 Shaking No Required temp No 450 nm 620 nm F Arrow keys and OK key START ON STOP OFF and IN OUT keys Figure 5 14 Keypad and display of the Multiskan GO Thermo Scientific Multiskan GO User Manual 29 Instrument User Interface Display and keys JA V gt mng m lt ch z a Pi T z E o Es un oo E Ss al ro Tl O OUT 30 Thermo Scientific Multiskan GO User Manual The user interface keys are detailed below The LEFT RIGHT UP and DOWN arrow keys navigate the menu structure move inside windows and change numeric values Repeat a selection by holding down the arrow key The OK key selects and edits the highlighted item The alphanumeric keys enter numerical data and text e Press a key several times to cycle through the characters e Pressing the O key enters a character space in text fields e Period Dot decimal point The CLEAR C key deletes text and numbers The F1 F3 keys select the action shown on the info text bar above the corresponding key The info text bar is updated according to the
28. ML 0010 10 00 45 00 12 50 12 50 600 4 40 ML 2010 ML 3010 ML 4010 ML 3010 B ML 4010 B GE Healthcare 80 2003 83 1 00 45 00 12 50 12 50 200 NA 80 2002 54 http www gehealthcare com 80 2003 85 5 00 45 00 12 50 12 50 1000 NA worldwide html 80 2002 57 80 2003 87 10 00 45 00 12 50 12 50 2000 NA 80 2002 58 80 2099 89 80 2109 80 80 2109 81 80 2002 77 10 00 45 00 12 50 12 50 800 4 40 80 2004 15 80 2100 13 80 2109 82 80 2002 95 10 00 45 00 12 50 12 50 400 2 40 80 2100 25 80 2109 83 80 2002 70 10 00 48 00 12 50 12 50 2000 NA http www spectrocell com Continued 82 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Manufacturer GE Healthcare Cont Starna http www starna com Thermo Fisher Scientific Code s 80 2003 98 80 2002 81 80 2100 22 80 2002 99 1 6 10 1 1 10 1 0 10 1 50G 10 9 1 10 9 0 10 9 SOG 10 9B 1 10 9B 0 10 9B SOG 10 29 1 10 29 0 10 29 506 10 21 6 10 21 1 10 21 0 10 21 506 10 298 1 10 298 0 10 29B 506 10 16 10 0 10 Z8 5 16 R 10 0 10 28 5 16 40 0 10 8 5 16 R 40 0 10 8 5 16 50 0 10 Z8 5 16 100 0 10 Z8 5 16 160 0 10 Z8 5 18 1 10 18 0 10 18 506 10 18B 1 10 18B 0 10 18B 50G 10 28 1 10 28 0 10 28 506 10 28B 1 10 28B 0 10 28B SOG 10 Pathlength mm 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 Height mm 48 00 48 00 45 00 45 00 48 00 48 0
29. N a NN ou N Ya daa 0 owu u an ON N on 44 NU o ou 99 99 So so 99 99 oo BO hu N NA JO N SU oO A rx A P aS D a uu a 20 0 a D ou aN 9a am BO WO ON BN NO NG oy o Ia 0 900 UN NO 2 00 00 o gt gt 99 909 99 oo oO oo o gt ut uo of an Ad Sp AN oo oo N ooo Ox N a oOo Nu o o 2 O 70 00 00 00 o b aan a a o N a its D o a Le a a 99 90 oo o cn N 0 Oo D D D D D D D D 0 1 99 oo ox oo oo oo oo Or o ou NIN on Plate Graph If a result table does not fit the view you can scroll the table with the arrow keys The icon at the top right of the view shows your current location in the table In multiple wavelength measurements the results are listed starting from the top for each well Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 39 Instrument User Interface Plate measurement Spectral measurement results are displayed one well at a time You can move between wells with the arrow keys The identified measurement peaks for each well are marked on the graph and listed beside it You can scale the graph between the well and plate maximum values with the F2 Scale key Press the F3 Close key to close the measurement results Press the F3 Results key to view the most recent measurement results if present Settings Session12 96 wells All 00 00 00 Shaking No Required temp No 450 nm 620
30. OOOO00000 O08 00 1 1 4 100000 100 1 11 00000 COPOOOODODOdO WOOOOOOOOOO00O Cancel 3 Press the Fl Accept key to accept the area selection 4 You can toggle between A and None with the F3 Select all Clear key Press AV if you want the entire plate to be measured or None to clear the plate Incubation and Yov can define the incubation and shaking parameters in the 5 Incubation parameters submenu shaking parameters Incubation parameters Duration hh mm ss 00 00 00 Shaking Interval Speed Medium Required temp C No duration L 22 0 C Accept Cancel The Incubation parameters submenu contains the following parameters e Duration hh mm ss The duration is the period between pressing the START ON key to the beginning of the measurement The shaking principle is visualized differently according to the shaking type e Shaking You can specify the shaking mode e No Shaking is off e Single Shaking for 10 seconds before measurement e Double Two shaking periods one at the beginning of the incubation period and one right before the measurement Both shaking periods have a duration of 10 seconds Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 37 Instrument User Interface Plate measurement e Interval Shaking in intervals the On time is 10 s and the Off time 10 s The last period before measurement is always on You can set the duration for interva
31. Operate the instrument only with software and hardware that is specifically designed or selected for it Thermo Fisher Scientific assumes no liability for the use of third party software applications A 10 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Chapter 2 General Description This chapter describes the main parts of the Multiskan GO instrument Instrument The front view of the Multiskan GO with cuvette instrument is shown Vi ews in Figure 2 2 A Cuvette port cuvette model Display ON key OFF key Keypad Plate measurement chamber door Figure 2 2 Multiskan GO with cuvette front view The rear view of the Multiskan GO instrument is shown in Figure 2 3 Power supply connector USB ports for printer and memory device Air inlet av Type label USB port for PC connection Transport lock bar storage position Figure 2 3 Multiskan GO rear view Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 11 General Description Plate carrier The side view of the Multiskan GO with cuvette instrument is shown in Figure 2 4 Plate carrier OUT Figure 2 4 Multiskan GO with cuvette side view Plate Ca rrier The Multiskan GO supports all common clear bottom 96 and 384 well plates with and without lids as well as low volume plates preferably a high base type Flat and C bottom shaped well bottoms are recommended U bottom plates are suitable but
32. Power consumption Heat dissipation User interfaces USB connections 2 0 compatible Light source Detectors Wavelength selection Microplate shaker Plates Cuvettes 66 Thermo Scientific Multiskan GO User Manual 285 mm W x 430 mm D x 260 mm H 11 2 W x 16 9 D x 10 2 H 10 8 kg 23 8 Ibs 10 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only 40 C to 0 C packed in transport packaging 25 C to 50 C packed in transport packaging 100 240 Vac 50 60 Hz Max 110 W typical operation lt 22 W Power Save lt 2 5 W 375 BIU max Standalone use 4 5 inch color display and keypad PC control Skanit Software PC Memory device port for data export External printer HP PCL5 compatible Xenon flash lamp Lamp lifetime typically 10 flashes 10 million 96 well microplates measured in fast mode Photodiodes for measurement and reference Monochromator Linear shaking with adjustable speeds The cuvette option does not support shaking 96 and 384 well formats Maximum plate height with lid 18 5 mm Maximum plate footprint 128 4 x 86 1 mm Dimensions 12 5 W x 12 5 D x 40 58 H mm Beam center height 8 5 mm Beam window gt 2 mm Thermo Fisher Scientific Technical Specifications Performance specifications Performance This section provides the performance specifications for the relevan
33. Precision The default fast mode is a measurement speed optimized mode that produces results that are sufficiently accurate for most uses The Precision mode takes more time to perform a measurement when the best possible measurement accuracy is required Use the DNA RNA session to perform a RNA dsDNA or ssDNA measurement on a cuvette Cuvette DNA RNA parameters Nucleotide Pathlength correction Accept Thermo Fisher Scientific Thermo Fisher Scientific User formula Instrument User Interface Cuvette measurement The DNA RNA submenu contains the following parameters e Nucleotide Select the nucleotide to be measured e RNA Calculate the RNA concentration e dsDNA Calculate the double stranded DNA concentration e ssDNA Calculate the single stranded DNA concentration e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength e Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Use the User formula session to perform a measurement using a user defined calculation on a cuvette r i Cuvette User formula parameters Pathlength correction a 1 0000 A1 nm 260 A2 nm 280 A3 nm 320 Accept The User formula submenu contains the following parameters e Formula Select the predefined formula e
34. Step 1 800 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm L Mode Fast Precision DNA RNA L Nucleotide RNA dsDNA ssDNA L Pathlength correction Yes No L Pathlength 0 1 10 0 mm User formula L Formula aM1 a A1 A2 a A1 A2 alA1 A3 A2 A3 La 0 0001 1 000 000 L Wavelength 200 1000 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Kinetic L Wavelength 200 1000 nm L Number of readings 1 100 L Interval 0 5 min L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Zeroing for cuvettes In order to perform measurements the instrument needs to initialize calibrate itself This process is called zeroing In microplate measurements the Multiskan GO performs zeroing automatically In cuvette measurements however the user must perform zeroing manually The instrument must be zeroed to determine the instrument zero absorbance level 0 000 Abs before the first measurement of each cuvette measurement type All measured sample absorbances will be measured in comparison to this baseline level The instrument s zeroing for cuvettes can be performed in several ways each having some benefits and pitfalls e The recommended zeroing process is to run the zero measurement twice first with an empty cuvette port and then with an assay blank cuvette o First zero with the empty cuvette port to set the baseline level of the instrument to zero Thermo Fisher Scientific Th
35. Thermo Fisher Scientific improves temperature uniformity to lt 0 5 C Thermo Scientific Multiskan GO User Manual 67 Technical Specifications Safety specifications Safety This section describes the safety specifications for the Multiskan GO specifications In conformity with the requirements 68 Thermo Scientific Multiskan GO User Manual instrument Multiskan GO bears the following markings Type 1510 24 VDC 4 A CE mark cT Vus monogram Multiskan GO conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2012 19 EU WEEE Directive Waste of Electrical and Electronic Equipment 2011 65 EU RoHS Directive Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equipment 2006 42 EC Machinery Directive Safety performance IEC 61010 1 2001 Ed 2 including US and CA National differences IEC 61010 2 010 2003 Ed 2 IEC 61010 2 101 2002 Ed 1 Particular Requirements for In Vitro Diagnostic IVD Medical Equipment The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains
36. Thermo Scientific Multiskan GO User Manual Rev 1 2 Cat No N10588 Copyright Copyright 2015 Thermo Fisher Scientific Corporation All rights reserved Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks Multiskan and Skanlt are registered trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots Screenshots may be slightly different on your system depending on the firmware version No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Power failure The system requires uninterrupted power supply in order to operate correctly Thermo Fisher Scientific has no responsibility whatsoever for system malfunctions arising from power failures
37. USB memory device monochromator Transmits certain wavelengths of the incoming light Consists of a rotatable diffraction grating and entrance exit slits optical density absorbance log 1 transmittance log h dimension O D photometer A device measuring absorbance or optical density photometry The measurement of the properties of light particularly luminous intensity Thermo Fisher Scientific power saving mode The instrument has a Power Save feature which lowers power consumption while the instrument is idle Refer to Power Save feature on page 24 and Configuration on page 52 self diagnostics Initialization tests and adjustments that the instrument performs before operation as well as autocalibration single measurement Synonym to endpoint measurement assay method The measurement is only done with a single reading transmittance The ratio of transmitted J and incident light 4 A zeroing To perform measurements the instrument needs to initialize calibrate itself The process is called zeroing and is automatically performed for microplate measurements but manually for cuvette measurements Refer to Zeroing for cuvettes on page 43 Thermo Scientific Multiskan GO User Manual 85 Glossary 86 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Index A absorbance 9 12 42 43 44 66 84 accessories 17 74 accuracy 2 45 56 66 append 50 appli
38. VE 5 Press the F3 Close key to return to the Settings menu For more information on the Settings menu refer to Settings menu on page 51 22 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Installation setting the instrument clock Setti ng the The instrument date and time settings may be incorrect on delivery and instrument clock should be checked and set before using the instrument To set the instrument date and time 1 Select the Settings menu tab using the arrow keys 2 Select Date amp Time with the arrow keys and press the OK key 3 Press the OK key to select Time 4 Enter the correct date and time with the arrow keys and the alphanumeric keys and press the OK key to confirm the selection Settings Date amp Time parameters 03 06 2010 10 08 11 Date format dd mm yyyy Time format 24 hour 220c F3 5 Press the F3 Close key to return to the Settings menu In the Date amp Time submenu you can also select the display formats for the date and the time Note Connecting the instrument to SkanIt Software automatically updates the instrument date and time according to the PC clock a Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 23 Installation Power Save feature Power Save feature Installing Skanlt Software for Multiskan GO 24 Thermo Scientific Multiskan GO User Manual The Multiskan GO has a Power Save feature which lo
39. aa es balai 12 oa Se ne oa 13 GU ato ea 14 ea 15 USB ports for memory device and printer 15 Skane SONWANE bet dsd Na 15 Instala ON Aa PA SA TAP AAA ANTA 17 W barto do Upon deleted dnd led 17 A el BB 18 Warnings and CU os 20 Operational Ena 21 Chano no tol lilas 22 Setting the instrument dok aa KE 23 Power Save feature ooocoocoWcoooco oooco co oo co o o o oo oooco o ooo 24 Installing SkanIt Software for Multiskan GO oo oooo 24 Routine OperatiOM o ooooo o ooo oo oo mo mo momo mmm 25 Se On nn EE EAE 25 Meas urine Ple emang dana 25 Measttinea Oven 26 5 ten do Wina ae aan ea 27 Instrument User Interface o oooooo oo oo oo 29 Depan an key aio 29 Ment A ee nan Sae 31 ASENCIO sen REA NS RN Es 32 Plate measurement a 34 Cuvette masi En sedal ds 41 A en ea Ma BA 51 Thermo Scientific Multiskan GO User Manual 7 Contents Chapter 6 Chapter 7 Chapter 8 Chapter 9 Appendix A Appendix B Appendix C 8 Thermo Scientific Multiskan GO User Manual Export Mle ormas iO 54 Maia eka eat 56 Regular and preventive Manta ads acid 56 Clean ofthe Intro adas 57 Disposal of materializan BBB 59 Decontamination protein 59 Refitting the transport load 61 o O NN 63 Mantas syto a 63 Disposal of the InsttO ment dai 63 Technical Specifications o ooo oooo oo oo oom oom 66 Generalspeci YON cells eaten ana an 66 Performance specifications aeieeiaii a e TA 67 Safety Spec cios 68 Troubleshoot
40. able range is 0 1 10 0 mm Shown only when pathlength correction is enabled Spectrum Use the Spectrum session to perform a spectral scanning measurement on a cuvette Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 45 Instrument User Interface Cuvette measurement DNA RNA 46 Thermo Scientific Multiskan GO User Manual Cuvette Spectrum parameters SiteMeter Mode End wavelength nm 1000 step nm 2 Pathlength correction No Cancel The Spectrum submenu contains the following parameters Start wavelength Enter the start wavelength for the spectral scanning measurement The available range is 200 999 nm End wavelength Enter the end wavelength for the spectral scanning measurement The available range is 201 1000 nm The end wavelength must be greater than the start wavelength Step Enter the measurement step for the scan The available range is 1 800 nm in increments of 1 nm The default is the value used for the previous measurement The last step wavelength will be the last possible wavelength that is smaller than or equal to the end wavelength Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Mode Select the measurement mode Fast or
41. active menu The FILE key opens the File menu for containing data management functions The HELP key offers information on the current view The IN OUT key moves the plate carrier into or out of the instrument The START ON key e Switches the instrument on e Starts a microplate or cuvette measurement e Accepts settings in submenus The STOP OFF key e Terminates the measurement process e Terminates the computer remote control e Cancels the current setting in submenus e Switches the instrument off press and hold Thermo Fisher Scientific Menu structure Session Plate format Area Measurement Thermo Fisher Scientific Sessioni All 000000 Shakeng No Required temp No 250 1000 nm 2 nm F to Results Instrument User Interface Menu structure The user interface is divided into the Plate Cuvette and Settings menus These main menus are displayed as tabs at the top of the display You can switch between the Plate Cuvette and Settings menus with the LEFT and RIGHT arrow keys when you are on the main menu level The Plate and Cuvette menus are used to define the measurement parameters for microplate and cuvette measurements The Settings menu contains universal instrument configuration settings Cuvotte i Settings 13 Plate i Guest Settings single FAN nen Fi Configuration Spectrum il Date amp Time DNA RNA Print header User formula j Status report Kinctio
42. and laboratory use EN 61326 2 6 2006 Product family standard Electrical equipment for measurement control and laboratory use in vitro diagnostic IVD medical equipment Test standards Performance limits EN 55011 2007 Class B 150 kHz 1 GHz EN 61000 3 2 2006 Class A EN 61000 3 3 1995 A1 2001 A2 2005 ANSI C63 4 2003 Class B 450 kHz 1 GHz EN 61000 4 2 1995 A1 1998 A2 2001 4 kV CD 8 kV AD Criteria B EN 61000 4 3 2002 A1 2002 3 V m 80 MHz 2 7 GHz Criteria A EN 61000 4 4 2004 1 kV Criteria B EN 61000 4 5 2006 2 kV line to ground 1 kV line to line Criteria B EN 61000 4 6 2007 3 Vims 150 kHz 80 MHz Criteria A EN 61000 4 8 1993 A1 2001 3 A m Criteria A EN 61000 4 11 2004 30 500 ms Criteria B 60 100 ms Criteria B 100 10 ms Criteria C 100 20 ms Criteria C 100 5000 ms Criteria C Thermo Scientific Multiskan GO User Manual 69 Troubleshooting Guide Handling abnormal situations Chapter 8 Troubleshooting Guide Note Do not use the instrument if it appears to malfunction A Hand ling If there is an abnormal situation during operation such as fluids spilling abnormal inside the instrument situations Switch off the instrument Figure 2 3 on page 11 2 Unplug the instrument immediately from the power supply Figure 3 11 on page 20 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 If the corrective measures taken do not hel
43. ative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Cleaning of the instrument on page 57 Clean the keypad surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used A Caution Never use acetone as it will damage the covers A Caution Do not autoclave any part of this instrument A Clean the instrument regularly as stated below Caution Although the Multiskan GO is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water a Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur A 1 Turn the power off and unplug the instrument 2 Use disposable gloves Thermo Scientific Multiskan GO User Manual 57 Maintenance Cleaning of the instrument 3 Clean the instrument outside and the plate carrier with a soft cloth
44. ats e Print header Enable or disable the header for printed reports and enter the header text e Status report View export and print the instrument status report Thermo Scientific Multiskan GO User Manual 51 Instrument User Interface Settings menu Configuration 52 Ihermo Scientific Multiskan GO User Manual A detailed overview of the Settings menu is shown in Table 5 7 Table 5 7 Settings menu parameters Settings menu Configuration L Language English Chinese French German Japanese Portuguese Russian Spanish L Power save No 5 15 30 60 L Export decimal delimiter Dot Comma L Reset to factory defaults Yes No Date amp Time LTime L Date format dd mm yyyy dd mm yyyy dd mm yyyy yyyy mm dd mm dd yyy L Time format 12 hour 24 hour Print header L Enable header Yes No L Header text Status report L Print L Export The Configuration parameters submenu allows you to select the user interface language Power Save setting and the export decimal delimiter You can also reset the instrument to its factory default values The submenu also displays the instrument s firmware version serial number and whether it is eguipped with a cuvette station Bo ke A PX Chinese Configuration Frangais French Deutsch German A BA Japanese 1 Multiskan GO Portugu s Portuguese Nan Power save Pyccrui Russian Firmware 1 00 12 Export Espa ol Spanish SN 1510 00000C decimal delimiter
45. c Chapter 9 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Table 9 12 and Table 9 13 Table 9 12 Instrument catalog number Code 51119200 51119250 51119300 51119350 Item Multiskan GO w o cuvette Multiskan GO w o cuvette Japan Multiskan GO with cuvette Multiskan GO with cuvette Japan Table 9 13 Codes for spare parts and accessories Code 5187139 5187149 N08217 N09997 NO4001 longer USB Item Quantity Skanlt Software for Microplate Readers Research Edition CD 1 skanlt Software for Microplate Readers Drug Discovery Edition CD 1 Power supply 1 Transport lock bar 1 USB A B device cable 1 8 m 1 cables available from PC stores Thermo Scientific Multiskan GO User Manual 75 Ordering Information List of spare parts and accessories 76 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Appendix A System Log Instrument name number User Date Comments PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 77 System Log 18 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Appendix B Certificate of Decontamination Name Address Tel Fax Instrument Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the return
46. cation 2 5 9 10 autocalibration 84 bandwidth 66 Brief User s Guide 74 C calculated results 48 changing the language 22 52 connecting to a computer 20 connecting to a printer 20 cuvette 3 9 10 11 12 13 14 15 26 27 30 31 32 33 41 42 43 44 45 46 47 49 50 51 53 54 55 57 58 59 62 65 66 74 84 glass or quartz 80 measurement 9 30 31 41 42 44 47 53 84 menu 26 31 32 33 41 42 plastic 83 port 10 12 13 14 15 26 27 42 43 55 57 58 59 02 port cover 26 results 47 sessions 41 47 49 50 station 26 32 43 51 D Date amp Time 23 35 50 51 52 58 decontamination 55 57 58 59 62 78 84 Certificate of 59 62 78 procedure 55 57 58 59 62 78 disposal of instrument 62 disposal of materials 58 DNA RNA 41 42 45 46 48 Thermo Fisher Scientific E endpoint measurement 38 48 84 environmental requirements 17 error 21 54 62 70 codes 70 messages 21 62 export file formats 40 49 53 exporting results 26 33 40 41 49 50 51 52 53 54 65 F File 30 33 40 49 50 G glossary 84 H header 15 41 50 51 52 53 54 heating 14 32 33 element 14 Help 30 33 69 how to decontaminate the instrument 55 57 58 59 62 78 how to install the Multiskan GO 17 18 21 67 how to maintain the instrument 55 how to switch on 25 importing data 54 incubation 9 14 33 34 37 parameters 34
47. cording to local regulations The Li battery must only be changed by an authorized service technician Instructions for changing the Li battery are described in the service manual A Thermo Scientific Multiskan GO User Manual 63 Maintenance Disposal of the instrument 64 Thermo Scientific Multiskan GO User Manual Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Pollution degree 2 see Safety specifications on page 68 Method of disposal Electronic waste Contaminated waste Infectious waste Regarding the original packaging and packing materials use the recycling operators familiar to you For more information contact your local Thermo Fisher Scientific representative Thermo Fisher Scientific Maintenance Disposal of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 65 Technical Specifications General specifications General specifications Chapter 7 Technical Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program Table 7 9 through Table 7 11 Table 7 9 General specifications General specifications Overall dimensions Weight Operating conditions Transportation conditions Storage conditions Mains power supply
48. dampened with water or mild detergent YAN aN 4 Ifyou have spilt infectious agents on the instrument decontaminate sa the instrument Refer to Decontamination procedure on page 59 A Cleaning the sample holder Plate carrier 58 Thermo Scientific Multiskan GO User Manual Caution Do not use any solutions containing oxidative chemicals such as hypochlorites and peroxides or strong bases on any of the anodized aluminum surfaces plate carrier cuvette port as this may cause permanent damage to the finish A To clean the sample holder follow the instructions below To clean the plate carrier Caution Keep the instrument plate carrier clean to avoid dust and dirt from entering the measurement chamber Figure 2 2 Clean the plate carrier surface at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution soap solution or 70 ethanol Wipe up spills immediately Do not use formaldehyde or strong alkalines A Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur A 1 Turn the power off and unplug the instrument 2 Use disposable gloves 3 Check that the plate clamp secures the plate when the plate is moving into the instrument 4 Clean the plate carrier with a soft cloth dampened with
49. ditional room for cables covers etc There is sufficiently room behind the instrument to enable disconnecting the device The ambient air is clean and free of corrosive vapors smoke and dust The ambient temperature range is between 10 C and 40 C The humidity is low so that condensation does not occur relative humidity is between 10 and 80 Caution Do not operate the instrument in an environment where potentially damaging liquids or gases are present A installation ste ps This section describes the installation steps that have to be carried out before operating the instrument Caution Leave the instrument to sit for at least three hours before installing and switching it on to prevent condensation causing a short circuit A Removing the To remove the transport lock transport lock Caution Do not attempt to operate the instrument with the transport lock in place Figure 3 7 A Figure 3 7 Multiskan GO with the transport lock in place l 18 Thermo Scientific Multiskan GO User Manual Open the measurement chamber door by pushing the bottom edge and pulling the upper edge Figure 3 8 Thermo Fisher Scientific Thermo Fisher Scientific Installation Installation steps Figure 3 8 Transport lock bar with warning tag 2 Unscrew the transport lock bar by turning it counterclockwise Use a suitable flat head screwdriver if necessary 3 Pull the plate carrier until it is fully out of the i
50. e measurement eo Measurement results The instrument status report contains the following information e Print header e Instrument information name serial number and firmware version e Startup test results e Memory usage e Operating statistics e Temperature information e Detailed technical instrument data e Error log Thermo Scientific Multiskan GO User Manual 55 Maintenance Regular and preventive maintenance Regular and Chapter 6 Maintenance Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary preventive maintenance Maintenance This chapter contains an outline of the points mentioned in the checklist checklist below Table 6 8 Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied Table 6 8 Maintenance checklist Item gt A 5 amp o S a gt Keep the instrument free of dust See Instrument care on page 57 Y Avoid disturbing any of the optical system components including Y optical covers See Instrument care on page 57 Wipe away spilled saline solutions solvents acids or alkaline solutions Y from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Cleaning of the instrument on page 57 If any surfaces have been contaminated with biohazardous materia
51. ears briefly on the display 3 Press the START ON key 4 Check that the plate carrier moves in and the measurement chamber door closes properly The instrument starts the measurement After the measurement the plate carrier comes out and the measurement results are displayed No error messages should appear on the display 5 Press the F3 Close key to close the result table Thermo Scientific Multiskan GO User Manual 21 Installation Changing the language Chang ng the You can select the preferred language for the user interface The user lan uage interface errors and warnings printouts and exported data are available g g in the selected language To change the user interface language di fe 1 Select the Settings menu tab using the arrow keys A Y OK 2 Select Configuration with the arrow keys and press the OK key 3 Press the OK key to select Language f PX Chinese Configuration Fran ais French Deutsch German Asa Japanese Portugu s Portuguese ra oo Power save Pycckwit Russian Firmware 1 00 12 Export Espa ol Spanish SN 1510 00000C ba Es a ok Seige oo zo z decimal delimiter Ba ia Yes Reset to factory defaults Cancel 4 Choose the user interface language from the list using the arrow keys and press the OK key to confirm the selection The available languages are English default German French Spanish Portuguese Russian Chinese and Japanese A
52. ed items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 79 Certificate of Decontamination 80 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Manufacturer Hellma http www hellma worldwide com Continued Thermo Fisher Scientific Appendix C Example of Available Cuvettes These cuvette lists are given only as an example Table 9 14 and Table 9 15 There are many other cuvettes available that can also be used with the Multiskan GO The Multiskan GO can be used with cuvettes that have external dimensions of 12 5 W x 12 5 L x 40 58 H mm and are compatible with a beam center height of 8 5 mm Table 9 14 Glass or quartz cuvettes Code s Pathlength Height Width Depth Volume Meas mm mm mm mm pl position height mm 105 810 UVS 1 00 53 00 12 50 12 50 39 8 5 100
53. epeatability 66 resolution 66 Z zero 26 38 42 43 44 zeroing 26 38 42 43 44 48 84 Thermo Scientific Multiskan GO User Manual 89 Notes 90 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 91 Notes 92 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific
54. ermo Scientific Multiskan GO User Manual 43 Instrument User Interface Cuvette measurement o Then place the assay blank cuvette into the cuvette port and measure the absorbance of the blank sample with a normal measurement This reveals possible contaminations or other interferences in the assay blank samples o When the assay blank level is considered to be suitable with regard to the assay chemistry re zero the instrument using the blank cuvette e Alternatively perform the zeroing only with an empty instrument o First zero with the empty cuvette port to set the baseline level of the instrument to zero o Measure all samples including the blank sample This will also record the blank sample absorbance together with the sample results o Subtract manually the blank sample absorbance from all the other samples e The third possible way is to directly zero the instrument with the blank sample cuvette o Zero with the blank cuvette in the port to set the level of the blank sample to zero o Note that with this method possible contaminations in the blank sample are not recognized and this can therefore lead to incorrect subtraction of the blank levels Caution Do not perform zeroing with an empty cuvette An empty cuvette has four optical boundary surfaces between air and the cuvette material whereas real sample cuvettes have only two which leads to quite different beam behavior when the measurement beam is transmitted
55. fer to Export file formats on page 54 Figure 5 15 Multiskan GO with a USB memory device connected You can print measurement results directly to a connected USB printer In addition to session and run information the printed report also includes the print header if it has been defined in the settings Cuvette measurements are configured and performed in the Cuvette menu The Cuvette menu contains five predefined measurement sessions Single Spectrum DNA RNA User formula and Kinetic Cuvette Single Spectrum DNA RNA User formula Kinetic Results Cuvette sessions or measurement results cannot be stored in the instrument s memory You should export or print measurement results if you wish to use them later The instrument memorizes the most recent measurement parameters and results for each predefined session type Thermo Scientific Multiskan GO User Manual 41 Instrument User Interface Cuvette measurement The displayed instrument temperature indicates the cuvette incubator temperature A detailed overview of the Cuvette menu is shown in Table 5 6 42 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Cuvette measurement Table 5 6 Cuvette menu parameters Cuvette menu Single L Wavelength 200 1000 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Spectrum L Start wavelength 200 999 nm L End wavelength 201 1000 nm L
56. ge 4 Pull the plate carrier fully out of the instrument Figure 6 17 Figure 6 17 Plate carrier fully out of the instrument 5 Take the transport lock bar from the back of the instrument and locate it into the slot on the plate carrier and then fasten the red fixing screw Figure 6 18 Thermo Scientific Multiskan GO User Manual 61 Maintenance Refitting the transport lock Fixing screw da yA r Figure 6 18 Transport lock bar in place on plate carrier 6 Push the plate carrier fully into the measurement chamber with the transport lock bar and screw the rod into locked position 7 Close the measurement chamber door and make sure that the end of the transport lock bar enters the corresponding recess in the door and that the warning tag hangs out of the instrument Figure 6 19 Figure 6 19 Multiskan GO with the transport lock in place 62 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Packing for service Maintaining a system log Disposal of the Thermo Fisher Scientific Instrument Maintenance Packing for service To pack the Multiskan GO for service follow the instructions presented below e Inform about the use of hazardous materials e Remove any microplate or cuvette before decontamination Decontaminate the instrument e Refit the transport lock Refer to Refitting the transport lock on page 61 e Close the cuvette port and the measurement
57. hat the arrows on the transport packaging point upwards Place the instrument onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will void the instrument warranty A Retain the original packaging for future transportation The packaging is designed to ensure safe transport and minimize transit damage Use of insufficient packaging materials may invalidate the warranty Retain all instrument related documentation provided by the manufacturer for future use If you relocate your instrument or ship it for service refer to Packing for service on page 63 Check the enclosed packing list against the order Visually inspect the transport package the instrument and the accessories for any possible damage If any parts are missing or damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy When setting up your Multiskan GO avoid locations with excessive dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations It is recommended to place the instrument with the whole plate carrier above the table when the plate carrier is in the out position Thermo Scientific Multiskan GO User Manual 17 Installation Installation steps Make sure that The working area is flat dry clean and vibration proof and leaves ad
58. he USB memory stick printer error Please check the USB memory stick printer Contact service 24 The USB memory stick printer error The device memory stick printer connected to the USB port is not responding Try with another device Contact service 25 26 Did not find position calibration hole centerpoint Command parser optimization failure Contact service Contact service 21 Temperature reference is out of limits Incubation is not available Contact service 28 29 Instrument parameter memory error Parameters memory erase failure Contact service Contact service 30 Parameters memory write failure Contact service 72 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Troubleshooting Guide Error and warning codes 31 Incubator unable to reach target temperature Check the temperature setting and or restart the instrument Do not request incubation temperatures below ambient 2 degrees Contact service 32 Incubator not responding correctly Please restart the instrument Contact service 33 Diffraction filter not responding Contact service 34 Did not find 0 nm reflection peak Contact service 39 36 Did not find 882 nm calibration peak Grating constant calculated from the measured calibration peaks differs too much from the nominal value Contact service
59. ing Guide oooooo oom 69 Handling abnormal situations id 69 Error and warning Codes sena aa 70 USB memory A e a a lo Contacto Sencilla 74 Ordering Information oooooo oom 15 O Toba ea aa aa gt List Of Spate parts and Acceso 3 Systemi Lof aa RN 11 Certificate of Decontamination cscs 19 Example of Available Guvettes ooooooooo oo oom 80 Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Introduction to the Multiskan GO The Multiskan GO Figure 1 1 is a high quality monochromator based UV VIS spectrophotometer It is used in spectral scanning end point and kinetic measurements to measure absorbance in the 200 1000 nm wavelength range from appropriate 96 or 384 well plates with and without lids and various types of cuvettes The instrument allows incubation up to 45 C and shaking of the microplate The instrument can be controlled as standalone with straight forward internal software for quick and simple plate and cuvette measurements or by PC using SkanIt Software It can be operated in several languages English German French Spanish Portuguese Russian Chinese and Japanese In addition the Multiskan GO is robotic compatible and can be connected to plate handling devices such as stackers The unique Power Save function lowers its energy consumption The Multiskan GO can be used in a variety of research applications including nucleic acid and protein analysi
60. is too high Please restart instrument Contact service 11 Lamp or optics failure Please restart the instrument Contact service 12 Non volatile parameters lost Contact service 13 Attempt to set the instrument serial number when Do not try to set the instrument serial number it already has been set Thermo Scientific Multiskan GO User Manual 71 Troubleshooting Guide Error and warning codes 14 Instrument user parameter memory full The internal memory is full Take a backup by exporting the protocols runs Release space from the internal memory by deleting protocols runs from the internal memory 15 Startup initialization error Please restart the instrument Contact service 17 Well to well distance too short for fast measurement Ensure that the well distance in the PC software plate template is large enough or use precision measurement mode 19 Too high background light level The instrument must be operated with all covers on and lids closed Cuvette door might be slightly open Please check that cuvette lid is closed Please retry the operation Contact service 20 Too high measurement signal Please retry the operation Contact service 21 Measurement position check failed Please restart the instrument Contact service 22 The USB memory stick printer error Please check the USB memory stick printer Contact service 23 T
61. l Y disinfect with a mild sterilizing solution See Instrument care on page 57 Clean the case of the instrument periodically See Cleaning of the Y instrument on page 57 Clean the plate carrier and cuvette port when necessary See Cleaning Y the sample holder on page 58 Perform verification with the Spectrophotometric Verification Plate Y Cat No NO3394 Decontaminate the instrument when relocating the instrument or Y sending it for service See Decontamination procedure on page 59 Y service the instrument regularly See Cleaning of the instrument on page 57 and Maintaining a system log on page 63 Y depending on the laboratory conditions and the use and configuration of the instrument 56 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument care gt D p Cleaning of the Thermo Fisher Scientific instrument gt gt Maintenance Cleaning of the instrument Always ensure that the electricity supply in the laboratory conforms to that specified on the type label of the instrument To guarantee the continuous reliability and accuracy of the Multiskan GO avoid disturbing any of the optical system components A misalignment of the light path affects measurements e Prevent any liquid from entering the instrument e Keep the instrument free of dust and other foreign matter In the event of any damage contact your local Thermo Fisher Scientific represent
62. l shaking e Speed You can define the shaking speed Low Medium or High e Required temp C You can add a target temperature for the session which is a temperature reminder for the user The Required temp parameter works as a reminder to use a certain temperature for a specific session If the target instrument temperature is different from the Required temp or if it has not been reached yet the instrument will give a warning to ensure whether the measurement shall be executed The user can select to measure or cancel the session Settings temperature A Note The Required temp parameter does not affect the instrument Measurement The Measurement parameters submenu allows you to define the parameters measurement method wavelengths and mode The Multiskan GO provides three measurement methods Single wavelength Multiple wavelength and Spectral scanning measurements Measurement paramete Start wavelength n End wavelength nm step nm Cancel The Measurement parameters submenu contains the following parameters e Type Select the measurement type Single Multiple or Spectrum e Wavelength Enter the measurement wavelengths 38 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Plate measurement In a Single measurement you can define one wavelength In a Multiple measurement you can define up to three measurement wavelengths lt None gt
63. nstrument Figure 3 9 Fixing screw Figure 3 9 Plate carrier pulled fully out 4 Unfasten the red fixing screw and remove the lock bar from the Carrier 5 Push the plate carrier back into the instrument and make sure that the measurement chamber door closes properly Figure 3 10 Figure 3 10 Transport lock removed Thermo Scientific Multiskan GO User Manual 19 Installation Warnings and cautions Store the transport lock bar on the back of the instrument Figure 3 11 for use in future instrument relocation or transportation Connecting the To connect the power supply cable power supply cable Warning Never operate your instrument from a power outlet that has no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region A 1 Connect the power supply cable to the power supply connector and plug in the instrument Figure 3 11 2 Connect the power supply to a correctly installed line power outlet with a grounded conductor Power supply connector USB ports for printer Air inlet and memory device Type label Transport lock bar e USB port for PC storage position connection Figure 3 11 Connecting the power supply cable Connecting toa Ifyou are using a computer with the Multiskan GO connect the computer communication cable to the USB port marked USB PC Figure 3 11 Connecting toa Ifyou are using a printer connec
64. p contact authorized technical service or your local Thermo Fisher Scientific representative 70 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Error and warning codes are presented Troubleshooting Guide Error and warning codes When an error is detected the current operation is aborted After an error it is best to restart from the beginning after the problem has been fixed When an error occurs the error code and the suggested actions Code Explanation Suggested action 2 The instrument received illegal command Please restart both instrument and PC Contact the PC software vendor 3 The instrument received illegal command Please restart both instrument and PC Contact the parameter PC software vendor 4 Plate carrier position error Check that there is nothing hindering plate movement and retry the operation Contact service 5 Measurement head position error Check that there is nothing preventing free measurement head movement Please retry the operation Contact service 6 Grating position error Please retry the operation Contact service 7 Diffraction filter position error Please retry the operation Contact service 8 Diffraction filter home tolerance error Please retry the operation Contact service 9 A D converter offset voltage is too high Please restart instrument Contact service 10 A D converter noise level
65. rameters in the Cuvette menu 5 Zero the instrument by pressing the F2 Zero key For more information see Zeroing for cuvettes on page 43 6 Insert the sample cuvette into the cuvette port with its measurement windows aligned with the measurement direction arrow on the cuvette station Figure 4 13 Ensure that the cuvette is fully inserted Thermo Fisher Scientific Routine Operation shutting down Figure 4 13 Multiskan GO cuvette port 7 Press the START ON key to start the measurement 8 View the results of the measurement 9 Export or print the measurement data if desired Note Always keep the same cuvette orientation A Caution Ensure that you use a correct cuvette type with a measurement position height of 8 5 mm When you work in the UV range use quartz or other UV compatible cuvettes A When not using the cuvette port keep the cuvette port cover closed to protect it from dirt and dust Shutting down To shut down the Multiskan GO instrument 1 Remove any plates or cuvettes from the instrument 2 Switch off the instrument after use by pressing and holding the STOP OFF key for a few seconds Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 27 Routine Operation Shutting down 28 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Display and keys Active row Display Info text bar Function keys Alphanumeric keypad Thermo Fisher Scientific
66. s ELISA assays enzyme assays cytotoxicity and cell proliferation assays as well as apoptosis assays The Multiskan GO is available in the following configurations e Multiskan GO w o cuvette 100 240 V Cat no 51119200 and 51119250 Japan 96 and 384 well plate reading shaking and incubation e Multiskan GO with cuvette 100 240 V Cat no 51119300 and 51119350 Japan 96 and 384 well plate reading shaking and incubation Cuvette reading capability with incubation Note This manual covers the operation of the Multiskan GO with the cuvette instrument version If you have the Multiskan GO without the cuvette you can skip over the parts of the manual referring to cuvette use A Thermo Scientific Multiskan GO User Manual 9 Introduction to the Multiskan GO Intended use Figure 1 1 Multiskan GO with cuvette port A and without cuvette port B Intended use The Multiskan GO is a spectrophotometer that is used to measure photometric assays with defined 96 and 384 well microplates The Multiskan GO with the cuvette version can also measure photometric assays with cuvettes The Multiskan GO is intended to be used in research laboratories by professional personnel The Multiskan GO is designed to be part of an analyzing system for the end user who is responsible for validating the system to ensure reliable and safe results We recommend using Good Laboratory Practices GLP during the analyzing process Caution
67. splayed with up to five identified peak values Cuvette Spectrum 1 00 ui HAWA AY il Mi 200 300 400 500 600 700 800 900 1000 nm Export DNA RNA sessions the measured spectrum is displayed along with measured and calculated results Cuvette Nucleotide dsDNA A260 A3205 lt A280 A320 gt 50 4260 32 6 pg ml Thermo Scientific Multiskan GO User Manual 49 Instrument User Interface Cuvette measurement e User formula sessions the result of the user formula calculation is shown with the measured results Cuvette User formula Pe o 4 54 a 1 0000 Al nm A260 0 0501 A2 nm A260 0 1660 AS nm A320 0 2013 e Kinetic sessions the result curve of the kinetic measurement is displayed Cuvette Kinetic Curve Export Exporting results You can export measurement results to a connected USB memory device by pressing the F1 Export key in the result view For more information refer to Export file formats on page 54 To export the cuvette session e Export the current selected cuvette session by pressing the Fl Export key or File gt Export new file in the result view Nucleotide dsDNA A260 A3205 W lt A280 A320 gt soo AAN TI 50 A260 6 83 230 250 270 pg ml sport new file Export append Print 50 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific CUYOO006 TXT CUYOOO05 TAT CUYOOO04
68. stics passed after it has been passed The instrument is now ready for use diagnostics tests A Note The cuvette port cover must be closed during the startup self when data is transferred to the memory device A Note Do not switch the power off during startup self diagnostics or Measu ring a To measure a microplate plate 1 Load the microplate If the plate carrier is in first drive the plate carrier out by pressing the IN OUT key Always insert the microplate so that the Al corner is positioned in the top left hand corner of the plate carrier Figure 4 12 Figure 4 12 Multiskan GO with plate carrier out Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 25 Routine Operation Measuring a cuvette Measuring a cuvette 26 Thermo Scientific Multiskan GO User Manual Caution Ensure that you select the correct plate type An unsuitable plate may become jammed in the instrument When you work in the UV range use quartz or other UV compatible microplates A 2 Define the measurement parameters in the Plate menu 3 Press the START ON key to start the measurement 4 View the results of the measurement 5 Save export or print the results if desired To measure a cuvette 1 Open the cuvette port cover 2 Ensure that the cuvette port is empty or insert a cuvette with buffer to perform instrument zeroing 3 Close the cuvette port cover 4 Select the measurement session and define its pa
69. supply fluctuations 10 from nominal Installation category Il according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Thermo Fisher Scientific Thermo Fisher Scientific Technical Specifications Safety specifications Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation A EMC performance EN 61000 6 3 2007 Generic standards Emission standard for residential commercial and light industrial environments EN 61000 6 1 2007 Generic standards Immunity standard for residential commercial and light industrial environments EN 61326 1 2006 Product family standard Electrical equipment for measurement control
70. syringes disposable tips disposable gloves and so on as biohazardous waste Be cautious and always use gloves A If you have spilt infectious agents carry out the decontamination procedure Warning The decontamination procedure should be performed by authorized and trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another Example of decontaminants e Ethanol 70 Thermo Scientific Multiskan GO User Manual 59 Maintenance Decontamination procedure 60 Thermo Scientific Multiskan GO User Manual Virkon solution 1 3 Glutaraldehyde solution 4 Chloramine T Microcide SQ 1 64 Decon 90 min 4 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde as even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A To decontaminate the instrument l 10 Prepare the decontaminant Empty the plate carrier and cuvette port Ensure that you are wearing disposable gloves Switch off the power and disconnect the power supply cable Disinfect the
71. t specifications measurement technique and other instrument capabilities Table 7 10 Photometry Performance specifications Photometry Wavelength range Wavelength resolution Wavelength accuracy Wavelength repeatability Bandwidth Read out range Linearity 450 nm Accuracy 450 nm Precision 450 nm stray light Measurement speed from A1 back to A1 Spectral scanning speed 200 1000 nm 1 nm 2 nm 0 2 nm lt 2 9 nm Up to 4 Abs Plate Cuvette 0 2 5 Abs 2 0 2 5 Abs 2 1 0 0 003 Abs 0 2 0 Abs 1 0 0 003 Abs 0 2 0 Abs 2 0 2 0 2 5 Abs 2 0 2 0 2 5 Abs SD lt 0 003 Abs or CV lt 0 5 SD lt 0 003 Abs or CV lt 0 5 Precision mode SD lt 0 003 Abs or CV lt 1 0 Fast mode 0 05 230 nm 96 well plate Fast mode 6 s Precision mode 27 s 384 well plate Fast mode 10 s Precision mode 92 s 10 s from 200 to 1000 nm with 1 nm steps 96 well flat bottom plate Quartz semimicro cuvette with 4 mm beam window Table 7 11 Incubator Performance specifications Incubator Range Liguid warm up time Performance at 37 C Incubation with lid Ambient 2 C to 45 C Liquid warm up time lt 55 min from 25 C to 37 C uncovered 96 well plate 200 ul water well Mean temperature of wells 0 5 C Plate temperature uniformity lt 1 0 C with uncovered 96 well plate Using a lid increases the liquid temperature approximately 0 5 C and
72. t it to the USB port marked printer USB PRINTER Figure 3 11 Warni ngs and This instrument is designed to be safe for use When correctly installed cauti ons operated and maintained it will present no hazard to the user 20 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Electrical A A Operational Thermo Fisher Scientific check Installation Operational check The following recommendations are given for added user safety Ensure that the power supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The power plug should only be inserted into a socket outlet with a protective ground contact Never use an extension cable without a protective ground wire Warning Only authorized technical service personnel are allowed to open the instrument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening it A Warning Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply A Before you put the instrument into use perform the following operational check 1 Switch the instrument on by pressing the START ON key The instrument proceeds to perform the self diagnostics 2 Check that the plate carrier comes out of the instrument and the Self diagnostics passed text app
73. the current view Help Press the HELP key to open the help window and use the arrow keys to scroll the view if necessary Press the OK key to close the help window and return to the previous view Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 33 Instrument User Interface Plate measurement Plate Microplate measurements are defined and performed in the Plate menu measurement Settings Session Session12 Plate format 384 wells ES Area A1 H10 i D gt Incubation 00 02 00 Shaking No Required temp 30 C dy Measurement 400 700 nm 10 nm F 24 22 0 The Plate menu contains settings for the session name plate format measurement area plate incubation and shaking and the measurement type A detailed overview of the Plate menu is presented in Table 5 3 Table 5 3 Plate menu parameters Plate menu Session Session name indicates unsaved results L Select session to open Plate format 96 or 384 wells L 96 wells L 384 wells Area All One well e g A1 Area e g A1 D4 L Selected area All One well e g A1 Area e g A1 D4 Incubation Duration Shaking mode Required temperature L Incubation parameters L Duration hh mm ss L Shaking No Single Double Interval L Speed Low Medium High L Required temp No Temp e g 30 Measurement Measurement information L Measurement parameters L Type Single Multiple Spectrum L Wavelength Wavelength 1
74. to use FAT16 or FAT32 formatted USB memory devices It is not recommended to use multiple drive USB memory devices that contain virtual CD drives The multiple drive USB memory devices normally contain one or more removable disk type memory spaces but can also contain virtual CD drive type memory spaces The Multiskan GO does not support virtual CD drive type memory spaces It is possible to check if there is a virtual CD drive present when inserting a multiple drive USB memory device into the PC USB port 1 Select My Computer and note the new drives that appear in the list when the multiple drive USB memory device is inserted 2 Check all the appearing items by using the right click mouse menu and selecting Properties 3 The item type Removable Disk is stated for the USB memory device in the General sheet It is compatible with the Multiskan GO if the File system is FAT16 or FAT32 4 If the item is a virtual CD drive then the type will be CD Drive This may cause the USB memory device to be incompatible with the Multiskan GO Always attach the Multiskan GO Instrument Status Report when contacting service This can be generated by selecting Settings gt Status Report from the Multiskan GO user interface or in the SkanIt Software Home gt Settings gt Instruments gt Edit instrument parameters gt Report Thermo Fisher Scientific Multiskan GO List of spare parts and accessories Thermo Fisher Scientifi
75. trolled by a thermal sensor The incubation temperature range is from ambient 4 C to 45 C Note that the liquid warm up is considerably slower than the incubator warm up The cooling fan is used to maintain the instrument temperature near ambient conditions It is automatically activated when the instrument measurement is ongoing The fan can also be activated for faster instrument cooling by setting the target temperature lower than the current plate incubator temperature The lowest possible temperature is slightly above ambient Thermo Fisher Scientific General Description Shaker Shaker The linear shaker of the plate carrier operates at different adjustable speeds Table 2 2 If the sample physical properties differ considerably from water or if U bottom plates are used ensure that the proper microplate shaking parameters are used The cuvette port does not support shaking Table 2 2 Shaking speeds Speed name Speed Low 5 Hz amplitude 15 mm Medium 11 Hz amplitude 3 mm High 20 Hz amplitude 1 mm USB ports for The USB port can accept an external memory device or printer Fi 2 11 memory device 2740 page 11 and printer You can transfer any measurement data to the PC with a USB memory device It is recommended to use FAT16 or FAT32 formatted USB memory devices It is not recommended to use multiple drive USB memory devices that contain virtual CD drives For more information refer to Chapter
76. use they offer better performance Ensure that the selected cuvette type has a clear window within the measurement window and that the measurement windows of the fully inserted cuvette are aligned with the measurement direction arrow on the cuvette port Figure 2 5 Figure 2 5 Aligning the measurement windows of the inserted cuvette Optical system The optical system of the Multiskan GO instrument is shown in Figure 2 6 Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 13 General Description Incubator Incubator Cooling fan 14 Thermo Scientific Multiskan GO User Manual Sy Moving measurement head Mirror optics gt o mn Detector ke y Microplate reading station L Entrance slit y Fiberoptic link Exit slit Xenon flash light source Fiberoptic link Cuvette reading station Rotating holographic grating SCmaasOptcs dat amp 12 2000 Ju Figure 2 6 Multiskan GO with cuvette optical system Both the plate carrier and the cuvette port have their own incubator The incubation temperature setting is the same for both the plate carrier and the cuvette port The plate measurement chamber incubator consists of thermal sensor controlled incubator zones with two at the top and two on the bottom of the incubator chamber The incubator design prevents condensation forming on the microplate lid The cuvette port incubator is equipped with a separate heating element con
77. water or mild detergent 5 If you have spilt infectious agents on the plate carrier decontaminate the instrument Refer to Decontamination procedure on page 59 Thermo Fisher Scientific Cuvette port AN Instrument clock battery Disposal of materials Decontamination Thermo Fisher Scientific procedure Maintenance Disposal of materials Warning Ensure that the bottom of each microplate is dry Fluid on the bottom of a microplate may present a contamination hazard Use good laboratory practices when handling any hazardous materials A To clean the cuvette port follow these instructions Clean the cuvette port with a Q tip cotton swab moisturized with water mild detergent or 70 ethanol Caution Do not clean the cuvette port Figure 2 2 if a cuvette has broken If the cuvette port becomes completely contaminated contact authorized technical service A The instrument electronics contains a battery for keeping the date and time information The battery is only in use when the power supply is disconnected The typical lifetime of the battery is 2 5 years If the instrument starts losing its date and time information call service Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used disposable plastic plates and cuvettes
78. wers power consumption when the instrument is idle You can control the feature in the Settings menu where you can set the idle period before activation or turn the feature off In the power saving mode the stepper motors and incubators are shut down the flash lamp is off and the display is dimmed The state of the user interface is stored To reactivate the instrument press any instrument key The SkanIt Software reactivates the instrument automatically The user interface returns to the previous state To change the Power Save parameter 1 Select the Settings menu tab using the arrow keys 2 Select Configuration with the arrow keys 3 Select Power Save and press the OK key to change the parameter Select No to inactivate the Power Save feature For information on installing SkanIt Software for Microplate Readers refer to the Skanir Software for Microplate Readers User Manual Cat no N16243 When the instrument is connected to SkanIt Software the Remote mode active text is shown on the display The STOP OFF key on the keypad can be used to disconnect the computer remote control Thermo Fisher Scientific Chapter 4 Routine Operation Switchi ng ON Before switching the instrument on check that all the cables are properly fitted according to the installation instructions Switch the instrument on by pressing the START ON key The instrument proceeds to perform the self diagnostics and displays Se f diagno

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