Installation Guide - DIESSE Diagnostica Senese
Contents
1. MATIC VES Installation Guide DELTA _LIMIT 11 Minimum derivative value used by Method 2 SH THRESHOLD z100 Sample High detection threshold used by both methods START POINT M1 180 Right most point analyzed by Method 1 END POINT M1 0 Left most point analyzed by Method 1 START POINT M2 140 Right most point analyzed by Method 2 END POINT M2 0 Left most point analyzed by Method 2 DIFF 5 10 Value added to the LIMIT VALUE to find the step in Method 1 FILTER MIN ABS 20 Limiting filter applied in Method 1 DIGITS RATE 50 In method 1 as alternative condition to identify the step 1 2 MAX DIFF ESRz20 Maximum difference between the two ESR values PERC CORR ESR 600 Correction Value or ESR used by both methods RETRY 5 140 ESR final value above which the analysis is repeated NUM MAX RETRY 5 0 number of retry in case of error or values gt Retry Ves MAIN METHOD 1 Definition of the Main Method 1 2 MAX DIFF ERR 1 ERROR FLAG SL THRESHOLD 110 Sample Low Threshold START POINT M3 200 Right most point analyzed by Method END POINT M3 0 Left most point analyzed by Method The following section provides information about how to resolve some problems We recommend however also consulting the manual to use the software ReadViwer 1 0 When you change the parameters remember that END POI NT M1 and END POI NT M2 ought to be same2. 0 20 40 60 80 100 120 140 run 1 In this case the test has to be considered not correct since the repetition of a sample changes its state from normal to pathologic and vv Note 1 Before processing the samples assure that they are a fresh or stored at 4 c b wait for them to arrive at room temperature WARNING Avoid analyzing samples directly from the fridge the subsequent warming up will cause the test to be poorly reproducible for a progressive modification in the behavior of the sedimentation Note 2 Before reporting the obtained values in a test of reproducibility pay attention of possible error flags present see paragraph on errors 32 of 33 Revision 2 00 of O4 11 2009 VES Matic RIECCE Installation Guide Use the following evaluation chart ALL SAMPLES IN THE LIMITS 12 NO WITHOUT ERROR CODE TEST PASSED TEST FAILED PERFORM MORE CONTROLS OR SEND 10g FILE TO T A Appendix A Suggested procedure to Set sample LOW code 1 Prepare 3 tubes with 1 5 mL of blodd 2 Enable the reading view page insert the password 131313 like when you enter in the service menu before to press START SM MODE 3 after the mixing of the samples check the values for READ1 The value for SL THRESHOLD parameter will be the average obtained value from vescube minus 5 units E g 131 128 133 set in vescube ini file SL THRESHOLD 125 Michele Meloni Francesco Cocola 33 of 33 Rev
3. ZA Fig 2 Labelling compatible heights with Ves Matic Cube 80 The reading group sensors are able to read correctly the sedimentation rate inside each sample following the reading axis passing through a maximum of three label layers thus are allowed only two labels attached to the tube which must be staggered at least of 90 degrees Fig n 3 Reading Axis Reading Axis i Light Receiver 54 Layer 54 Layer Barcode Label Barcode Label am 2 Layer 27 Layer Qa 2 Factory Label Factory Label 4 2 2 777 Light Emitter Tube cross section 1 Layer Layer Max 3 layers on the Reading Axis Fig 3 Labelling Correct labels placing on sample tube 14 of 33 Revision 2 00 of 04 11 2009 MATIC nIECCE VES Installation Guide NOTICE Verify before loading the instrument that the labels adhere perfectly to the tubes otherwise the unattached parts could cause frictions during the mechanical movements of the groups especially inside the inserting ejecting and sorting module creating inserting and ejecting problems in the analitycal chain and possible blocks of the reading sensors In the following pictures some WRONG labelling examples are displayed An incorrect labelling could cause mechanical blocks and or reading problems to the Opto Electronic Sensors NO Mmm imr AMT Reading Axis 4 N
4. You can download the software at the following address http www diesse it support ves vescube Please note that access to the site is submitted to a password in case you do not have your password please contact salesoffice diesse it After downloading unpack the file Zip it is not necessary to create a specific directory Evaluation Flag type Max If max e If gt max o Check the ti the tube Volume and If the check results label 27 of 33 Revision 2 00 of 04 11 2009 VES Installation Guide Procedure 5 Precision between RUNS visually ok send the log txt file to the tech assistance Check the tube If the check results visually ok send the log txt file to the tech assistance Check tube and M1 M2 reading If the check results visually ok send the log txt file to the tech assistance If a lot of M2 values are O check the volume If the sample volume is near to 1 5 ml try to increase START POINT M2 of 20 units otherwise if the sample volume is near to 4 ml decrease START POINT M2 of 20 units Check tube and M1 M2 reading If the check results visually ok send the log txt file to the tech assistance Volume and label Label and calculation Label and calculation samples must be processed TWO TIMES using the same Ves Matic Cube 200 with temperature correction active The instrument whic
5. it own NE Xi imr iun d fo f AE TT DI ELI en rl d x Ui KI il N ui VES Mie 3096 94348 15 10 2008 oo Access password gt 131313 After the password the software comes back to the main windows 20 of 33 Revision 2 00 of 04 11 2009 MATIC VES Installation Guide Procedure 4 ANALYSI S RESULTS CALCULATI ON ALGORI THMS and Error Evaluation The reading sensors that measure the height of the blood inside the tubes are composed by an high power white led and an analogue light sensor The reading procedure executed by Reader 1 and Reader 2 is the following The Reading sensor goes to the end position that corresponds to the top position immediately under the cap of the tube The LED is powered ON The sensor starts to move slowly from top to bottom During the movement at predefined fixed space intervals the Light Sensor output signal is acquired and stored The acquisition is completed when the sensor reaches the home position The Tablet PC requires to the Motor Driver Board that controls the Reader Unit the acquired light samples to elaborate them and to calculate the blood level An example of the reading data is shown in the following images Actual Reading Data Derivative of Actual Reading Data E Filtered Reading Data m
6. o Rad I N T E N 5 Y Fig 5 1 READINGS In fig 5 1 the typical reading curve is shown On the vertical axis there is the Light Intensity measured by the light sensor On the horizontal axis there are the various readings starting from the first R1 that corresponds to the upper reading point to the last R231 that corresponds to the lower reading point In the next images the correspondence between the reading curve and the physical blood sample are explained in detail 21 of 33 Revision 2 00 of O4 11 2009 MATIC WIESE VES Installation Guide Top of the Tube DIOE pont of blood Bottom of the Tube Fimo Ir Hit me t e eS eA Fig 5 2 Start point of blood In fig 5 2 are explained the various points of the readings curve with their physical correspondence on the tube The above curve is related to the First Reading of the sample that is immediately after the Mixing This is the Initial blood level 22 of 33 Revision 2 00 of 04 11 2009 MRTIC VES Installation Guide Top of the Tube Final Start point of blood Bottom of the Tube Gn I N 5 READINGS PLASMA Fig 5 3 Initial blood level Final reading level In fig 5 3 is represented the same sample of fig 5 2 but read after the sedimentation time of 20 minutes In this case the Red Cells are deposited on the bottom of the tube and abo
7. rd SL Barcode Label 15 of 33 Revision 2 00 of O4 11 2009 MATIC RIECCE VES Installation Guide Fig 4 Labelling Incorrect label for Ves Matic Cube 80 16 of 33 Revision 2 00 of O4 11 2009 VES Installation Guide Materials 1 Ves Matic Cube 80 2 Fresh blood samples collected from the laboratory suggested N 50 Note Each instrument executes the analysis of the samples directly from test tubes being used on the blood cell counter in the laboratory it is therefore not necessary to do a double drawing nor a transfer of biological material Procedure 1 Check VESCUBE INI parameters Enter in the Service Menu as described Amalas Mod wee Loader Wod View Apri amp portelio 2T Samples ai JI iy Em 7 TIE uL f 2 al Diren Date Time BE ml NM I i Li gt gt 3096 943 48 15 10 2008 Archive Access password gt 111111 17 of 33 Revision 2 00 of 04 11 2009 DIESE VES Installation Guide 0 00 EEP settings m 0x0 Postioner Ox10 ClampH 004 Mixer C 11 C 0x05 Reader1 C 0x13 RackPh O Ox06 Reader2 0x20 Transponder C 0xD8 RackShifter _
8. Pc Barcode Reader remember to always do this while the instrument is switc hed off it is necessary to verify compatibility see the relative user manual with the specifics indicated in chapter 7 of the Operator Manual and venfy that the ground connection between them is unintemupted Connection with an extemal PC is possible with specific software Activesink The operator has to be trained to ensure awareness of proper procedures restrictions and wamings indicated in this guide in addition to the required individual laboratory safety procedures consumables used cleaning solutions for the cleaning of the instrument has to be The material for the security of the operator gloves container for the disposal of the always available The collocation of the instrument has to follow the guidelines indicated in paragraph 5 IT IS ABSOLUTELY PROHIBITED to remove or modify the security and protection devices of the instument Revision 2 00 of 04 11 2009 Page 5 of 33 VES Installation Guide 4 UNPACKING executed by atleast two people During the movement of the device avoid janing and excessive inclination that Wy Given the size and weight of the device any movement of the instument has to be could damage the instrument Unpac king of the instrument Execute the phase of unpacking as described and shown below 1 Remove the screws of the wood box 2 Remove the cap ofthe box Fi
9. back in a right to left direction the algorithm looks for a step in the curve This step is assumed to be the Starting Point of blood This Step Point is the blood level in Method 1 Applying this method for both Reader 1 and Reader 2 the two levels of blood are detected and an ESR value is calculated this ESR is the Method 1 ESR value Method 2 Method 2 is based on the first derivative calculation for all the points of the curve This calculation generates a new curve the blue one on the above figures By analyzing this new curve starting from the left most point the software looks for the first higher rate falling edge the point of this falling edge is assumed to be the Blood Level of Method 2 Applying this method for both Reader 1 and Reader 2 the two levels of blood are discovered and an ESR value is calculated this ESR is the Method 2 ESR value Method 3 Revision 2 00 of O4 11 2009 VES nirccr Installation Guide Method 3 is based on a particular function of squaring signal to eliminate all spikes present The software draws a new curve and after applies again 1 M2 data To activate the error message set the following param at 1 M1M2 MAX DIFF ERR 1 In the Ves matic cube it s possible to have 4 different alert data HI GH LOW Res ERR After the first run is important to verify GH For each HIGH message instead of the result check the blood s volume in the tube Procedu
10. Peripheral Flags Error Flags Motor Flags Busy iotor Busy Timeout RackPh sele cl Jl B Resa Harcode Program Read Parameters default values for Vescube ini READ PARAM DELTA LIMIT 11 SH THRESHOLD 100 START POINT M1 180 END POINT M1 0 START POINT 2 140 END POINT M2 0 DIFF DIGITS 10 PERC FILTER MIN ABS 20 DIGITS RATE 50 M1M2 MAX DIFF ESR 20 PERC CORR ESR 60 RETRY VES 140 NUM MAX RETRY VES 0 MAIN METHOD 1 M1M2 MAX DIFF ERR 1 SL THRESHOLD 110 WHIT M2 NOT ZERO 0 OVERDILL ENABLE O 18 of 33 Revision 2 00 of O4 11 2009 VES Installation Guide Note Using the Greiner Vacuette tubes end sarstedt tubes it is suggested to set the parameter START POINT 2 as follows START POINT M2 120 END POINT 1 end END POINT M2 must be equals Procedure 2 Log file activation It s very important to activate the Log txt In the Vescube INI file check and eventually change the following parameter LOG ENABLED 1 19 of 33 Revision 2 00 of O4 11 2009 Installation Guide VES Procedure 3 Values for 1 and M2 activation Procedure directly show the values for 1 and M2 obtained from readerl and reader2 on video during the run Open the Service Menu as described o AIT Lowder bod view Daia Wiew Apri Sportello E m e Sampha rr
11. STLOAD SAMPLE FROM RACK end push exec e Ifthe clamp don t conectly goes on the tube you can change the parameter CLAMPH RACK POSI e If the clamp don t conectly pick up the tube from the rack undemeath the cap you can change the parameter LOAD CLAMPV RACK before making the adjustment to this parameter check the Home Offset of the unit 9 In the menu Procedures select the TEST SAMPLE INSERTION end push exec e If you have a problem can change the parameter of vescube ini INSERTION CLAMPV CHAIN INSETRROEN T VCHAIN CLAMP INSERTION CLAMPV OVER INSERTION TOVER CAP CLOSE CLAMP INSERTION CLAMPV CAP DOWN 10 From the Procedures section TEST SAMPLE EXSTRACTION end push exec e If there is e problem whit the positioning horizontal of the clamp ex when the clamp go down it knock on the sensor you must check the Home Offset of the unit e If the clamp don t conectly pick up the tube from the chain undemeath the cap you can adjustthe parameter EXTRACTION CLAMPV CHAIN for go further down it 11 Now Select the Procedure TEST UNLOAD SAMPLE TO RACK and press Exec e If there is a problem you can adjust the parameter CLAMP RACK POSI end UNLOAD TOPEN CLAMPV 12 Exit from the Service menu by pressing BACK 13 Download the rack 14 Press the Start Button to execute the Reset procedure 15 Load a Classifier Rack whit 5 samples and when requested insert the ID code of the rac
12. VES 2e Installation Guide Revision 2 00 of O4 11 2009 English nireer Diagnostica Senese S p A MATIC VES Installation Guide SUMMARY 1 TECHNICAL 1 3 2 STORAGE AND 4 4 2 0 2 1 4 4 3 PREPARATION AND CHECKS BEFORE 5 4 4 cmm xT HH 6 PACEMINE 9 6 M ee 10 7 FUNCTIONAL STARTUP 00 2 2 2 11 8 ANALYTICAL 0 0 0 0 002074 2 13 Revision 2 00 of 04 11 2009 Page 2 of 33 niECCcE VES MATIC Installation Guide 1 TEC HNIC AL SPEC IHC ATIONS Power supply Europe 230Vac 50Hz Usa Canada 110 120Vac 60Hz Absorbed electrical power 2 x 5 0A T 5 x 20 mm UL CSA 650 x 580 x 690 mm Ix hx p ct 5 from 15 to 35 Storage from 5 C to 45 C 5 3 g wit
13. e not subject to shocks or vibrations Furthermore it is advised that the VesMaticCube be placed far from possible generators of Electro magnetic waves for example fridges laboratory centnfuges and from instrumentation without the CE mark since they could affect the proper functioning of the instrument It is advised thata proper table be used that can support the weight of the instrument The table or bench top should not exceed 90cm in height to guarantee an ergonomically conect position forthe operator during the input of the commands on the Tablet PC The table or bench top where the instrument will be placed should allow enough space about 40cm on the sides of the instrument for the operator to easily introduce and extract the sample holder rack in the classifier module Fig 5 1 40 cm 40 cm Fig 5 1 Frontal view with the extensions for rack input Furthermore to be able to reach the connectors on the rear of the window and most of all to be able to quickly access the switch and the power cable in case of emergency itis necessary to maintain safe distance from the wall of atleast 20 c m from the back side of the instrument For operator safety do not place any matenals or objects such as paper or containers on or nearthe instrument Choose a position close to an undisturbed outlet free from electrical fluctuations Revision 2 00 of 04 11 2009 Page 9 of 33 n VES Instal
14. ease 2 24 points out the samples out of the above specified range showing LOW volume sample less than ml 1 5 and HIGH volume sample more than ml 4 0 This range don t apply at the sarstedt tube Total number of labels Labelling procedure and maximum number of labels compatible with the analytical System Ves Matic Cube 80 models are designed to work with a maximum of two labels attached to the same sample tube but they must not be overlapping Fig 1 Reading Axis Reading Axis Light Receiver 54 Layer 54 Layer Barcode Label 2 ayer 24 Layer EM fe Label Factory Label Factory Label Light Emitter Tube cross section Layer Layer Max 3 layers on the Reading Axis Fig 1 Labelling Maximum number of label layers compatible with Ves Matic Cube 80 13 of 33 Revision 2 00 of 04 11 2009 MATIC RIECCE VES Installation Guide The internal barcode reader placed inside the Preparor Module is e mechanically set to read labels attached to the tube at least mm 3 above the the start of bottom roundness Fig 2 e programmed to read barcodes placed at 90 degrees compared to the reading rays that is the barcode must be placed to cross horizontally the tubes vertical axis Fig 2 Q The reader nevertheless can correctly read sloping barcodes the slope must be within 5 and 5 degrees Fig 2 QD 2
15. er network 7 FUNCTIONAL STARTUP PROC EDURE Once the instrument has been placed on the table of the laboratory and all the locks has been removed please follow the procedure below forthe functional startup of the instrument l Switch ON the instrument 2 Push the button Open cover end rise up the upper coverand check that it remains in the open position 3 Remove the Front Panel end insert microswitc hes Two metallic Keys code 10345960 when you remove the panel the instrument show you the enor messages Use the Sound off button to silent the acoustical signal 4 On the touch screen press the Start button to execute the complete Software and mechanical Reset since is important that the cover is open you must push the mic roswitc h when press the button 5 Once the Reset procedure has been completed the Stop button is active and the Start button is inactive press the Stop button 6 Load a Classifier Rack whit 1 sample if in the laboratory the customer use the sarstedt tube is important that you insert this model of tube in the position 7A and when requested insert the ID code of the rack or read it with the extemal barcode reader 7 Enter in the Service Menu with the password 1 1 1 1 1 1 Revision 2 00 of 04 11 2009 Page 11 of 33 VES CUBE Installation Guide 8 Now from the service menu in the Procedures section located in the bottom nght comer of the display end select the TE
16. f the instrument has to be The Vesmatic Cube 80 is a precision instument and has to be handled as such Inappropriate handling can harm the intemal components and cause mechanical damage For the storage and handling of the instrument the environmental conditions specified in paragraph 1 must be strictly adhered Given the size and weight of the machine the transportation has to be camed out using all precautions necessary to avoid jamng and excessive inclination that could damage the instrument PACKAGE DIMENSIONS GROSS WEIGHT including EuroPallet Kg 5 PACKAGING WEIGHT including EuroPallet kg BO Keep the packadi complete with intemal pa for all subsequent transportation of the instrument Revision 2 00 of 04 11 2009 Page 4 of 33 MIECCE VES MATIC Installation Guide 3 PREPARATION AND 5 BEFORE INSTALLATION The following conditions must be enforced for the safety of the instrument and the operator requirements specifications and cument indicated on the electric power plate supplied with the instrument it is advised that the efficiency of the electrical system is periodically verified The network and relative outlets have to be outfitted with an efficient ground connection following the laws in force in the matter of electrical systems WY The power network installaton category Il must be compatible with the electrical Before making the connections with extemal instruments
17. g 4 2 3 Slide the box up from the top see lateral adhesive Remove the manual and the accessories contained in the box 5 Remove the base of the accessory compartment A Fig 3 5 the 4 lateral protections Fig 4 3 and the nylon bag that covers the instrument gt Revision 2 00 of 04 11 2009 Page 6 of 33 nireer VES Installation Guide 6 Apply the supplied handles to the instrument to execute the transfer of the instrument as shown in sequence Fig 4 4 Fig 4 4 7 Check that the packing list conesponds with the supplied material during transport notify the delivery personnel and the Assistance Centre Should the instrument and or the accessories supplied tum out to be damaged Y Should there be any missing items notify the Assistance Centre Revision 2 00 of 04 11 2009 Page 7 of 33 Installation Guide Hr Nee V WX MATIC VES 10340531 2x 10338870 2x 8 20550510 2 21890370 1x 21880040 1x Page 8 of 33 Revision 2 00 of O4 11 2009 MIECCE VES MATIC Installation Guide 5 PLAC EMENT The environment intended for this instrument is the analysis laboratory For safety reasons and given the type of exams that it executes the instrument has to be placed far from sources of heat zones non accessible to liquids in environments free from dust and on perfectly flat work benc hes that ar
18. h is designed with the temperature correction activated relates the results to a temperature of 18 C according to Manley s Nomogram Graph 1 1 28 of 33 Revision 2 00 of O4 11 2009 VES en Room Temperature T 95 35 90 30 85 80 25 75 70 20 65 in RESULTS Installation Guide E S R Found ESR Cgarecied Graph 1 1 Manley s Nomogram MIECCE To evaluate the precision between runs use a very simple grah like the one in the follwing figure 140 120 100 80 60 run 2 40 20 20 40 60 run 1 80 100 theoric line 120 140 All samples have to stay within the red limits 29 of 33 Revision 2 00 of 04 11 2009 VES Installation Guide 140 120 100 80 theoric line 60 run 2 40 20 0 20 40 60 80 100 120 140 run 1 If one or more samples are out of range is important to evaluate the patological signifiance 30 of 33 Revision 2 00 of 04 11 2009 MATIC VES Installation Guide 0 1 140 120 100 80 60 run 2 40 0 20 40 60 80 100 120 140 In this case it is possbile ot accept the test as valid since there is no risk of invalidating the clinical meaning of the test 31 of 33 Revision 2 00 of O4 11 2009 MATIC DIESE VES Installation Guide Case 2 140 120 100 80 60 run 2 40 20
19. hout condensation Freescale i MX31 ARM11 Microprocessor Hash 128MB NAND 128 MB DDR RAM Display TFT 800x 600 colours with Touch Screen Microprocessor board on host Bus t 5 A d 5 9 5 2 2 E d 5 5 5 a 19 seconds in the analytical cycle 10 10 sliding blocks for housing and transporting the typical haematology racks Sample holder racks with 8x14 positions for storing processed test tubes Two pairs of opto electronic units Led amp Analogue Sensor Alphanumerical with thermal paper 58 mm wide 36 characters per row speed 20 mm sec 2 x RS232C 2 USB Host 1 USB Client 1 Sot Compact Hash CLASSI CEI EN 61010 1 Ed 2001 11 lt 22 2 No 61010 1 04 Ed 2004 07 UL61010 1 Ed 2004 07 EN 61326 Ed 2004 08 Relative humidity threshold Central unit Penpheral control unit Chain advancing step Sample insertion section Analysed sample collection section Optical units Protection Category Safety standards Installation category The safety and performance standards of the instrument are not quaranteed in the case of a different power su cable from the one supplied being used with the instrument Revision 2 00 of 04 11 2009 Page 3 of 33 niECCcE VES MATIC 2 STORAGE AND TRANSPORT Installation Guide executed by atleast two people V Given the size and weight of the device any movement o
20. ision 2 00 of O4 11 2009
21. k or read it with the handheld barcode reader 16 During the cycle check the conect working of the isrument a Identfication Barcode reading b Extraction insertion of samples clampV clampH step 17 c The conect wor of mixer d Try to check the movement of the Reader Sensor 1 amp 2 don t must touch the tube e Venfy the conect positions of the Rector 17 Atthe end of the exam press Stop and thet press Download Classif 18 Check the printout with all the tubes in the classifier rack comparing the barcodes and the positions 19 Remove the two metallic keys code 10345960 from the microswitc hes and place the front panel in its correct position 20 Start the Analytical Startup procedure Revision 2 00 of 04 11 2009 Page 12 of 33 VES meses Installation Guide 8 J ANALYTICAL STARTUP Before testing the system it is important to remember the 1 Volume range of the samples 2 Total number of labels Volume range of the samples Regarding the blood samples volume to be tested by the Ves Matic Cube 80 the following procedure must be followed strictly In order to have reliable accurate and precise results by this analytical system the blood sample volume must fall within the following range MINIMUM VOLUME 1 5 ML MAXIMUM VOLUME 4 0 ML This recommended volume is referred to all the different types of tubes we tested on the Ves Matic Cube 80 for example BD Greiner Terumo Software Rel
22. lation Guide relocation of the instrument be necessary a re venfication of the conditions listed in this paragraph would be required before using the instrument again Whenever the instrument will not be used or an extended period of time it is suggested that it be disconnected from the power source and covered 1 Never move the instrument after it is properly installed Should movement or 6 INSTALLATION 1 Place the instrument on a solid and flat surface as described in the previous paragraph Check the completeness of the supplied materials 3 Continue with the removal of the blocks of the Instrument as show in the following sequence NJ Revision 2 00 of 04 11 2009 Page 10 of 33 VES MATIC Installation Guide 4 Assure thatthe power switch is in the OFF lt lt 0 gt gt position before continuing Exec uted connections with the extemal instruments 6 Remove the tape blocking the window of the printer compartment and assure that the role of thermal paper is positioned conectly and free free it from possible presence of elastic security band used during transport 7 Install the rack insert extension as show in the photographic sequence gi 8 Connectthe plug ofthe power cable use the cable that is supplied with the instrument to the outlet on the nght side of the power switch on the instrument itself as shown in fig Connectthe plug of the power cable to the pow
23. ormally The Uncertain results will be sent to the host with the ESR value calculated and the Abnormal Sample flag set to one The host will recognize Uncertain results by the simultaneous presence of a valid ESR value and the error flag On the other hand if the ESR value is O and the error flag is set the Host computer will assume this as an ERROR If only one of the two methods is able to calculate an ESR while the other returns a zero the value calculated will be printed out also if the method that calculated it is not set as the Main method As in the case of high difference values if the ERROR Flag is set the result will be treated as Uncertain Value otherwise will be considered a valid result The two ESR values calculated by the two methods are processed as described in the following procedure If ESR M1 is lt gt to 0 and ESR M2 is lt gt to 0 and the difference between ESR M1 and ESR M2 is lt of a parameter in vescube ini file ESR is equal to the average of ESR M1 and ESR M2 If ESR M1 is to 0 ESR M2 is to O or the difference between ESR M1 and ESR M2 ERS is gt of a parameter in vescube ini file the software performs a M3 elaboration In this case the software will change the method m1 or m2 unable to evaluate the sample with M3 and will perform again the elaboration as previous described NOTE Parameters that affect the ESR final value 26 of 33 Revision 2 00 of O4 11 2009
24. re Fill a empty cell counter tube with 4 0 ml of water and compare this reference tube visually with all samples that have HIGH as result Is the Blood s volume is lower than the water check the labelling condition LOW For each LOW message instead of the result check the blood s volume in the tube Procedure Fill a empty cell counter tube with 1 5 ml of water and compare this reference tube visually with all samples that have LOW as result Is the Blood s volume is higher than the water check the labelling condition Note see appendix for a suggested procedure to set sample LOW Res ERR 25 of 33 Revision 2 00 of 04 11 2009 MATIC VES Installation Guide Method 1 amp Method 2 cross verification In the printout of the results and in the data sent to the host computer the following criteria are applied If the difference between the two ESR calculated by Method1 Method 2 is lower than the M1 M2 MAX DIFFERENCE the final ESR is the average value between those values and is a valid result Otherwise if the difference is higher than M1 M2 MAX DIFFERENCE the final ESR result will be the one calculated by the main method defined by the parameter MAIN METHOD If the ERROR FLAG is set this final ESR value will be considered as an Uncertain Value and a character will be printed near the result If the ERROR FLAG is not set the ESR will be considered a valid result and printed out n
25. ve them there is the Plasma Plasma is completely transparent for the reading sensor in fact the corresponding readings values are at the maximum level of light This allows the software to detect the final reading level that is that of the Red Cells Calculating the rate of level variation will bring to the final ESR value 23 of 33 Revision 2 00 of O4 11 2009 VES Matic Installation Guide Detecting the Start of Blood Point Two methods are used to elaborate the readings curves and detect the correct Start of Blood Point The two methods are completely independent the one respect to the other and are used to have a double security on the reliability of the readings in fact if the points calculated by the two methods are different a warning is signaled for that result to alert the user that it s an Uncertain result 24 of 33 Method 1 Method 1 first of all the minimum light point is detected on the curve Then using the parameter PERC_FILTER a limiting filter is applied to the curve so that all the values below the limit will be set equal to the limit itself The limit value is represented by the green line in the above figures Once that limit value has been found and the curve has been filtered using this value the curve is newly analyzed starting from the left most point until the first point with light value equal to the filtering limit Value Then starting from this point going
Download Pdf Manuals
Related Search