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User Manual (English)

1. UV Issue 2a 12 2000 19 01 98 PAR 0 281A 11 35 550 0nm SCAN TYPE INTELLISCAN To perform a scan set parameters ID TEST 1 MODE ABS and press RUN START 400 0nm STOP 600 0nm BANDWIDTH 2 0nm SPEED NORMAL DATA INTERVAL 1 0nm PEAK TABLE om SCAN GRAPH page GRAPH HIGH 2 000 GRAPH LOW 0 000 19 01 98 SCAN GRAPH Ga l LAMP CHANGE 825nm 11 85 ID TEST 1 550 0nm OPERATOR Name UVCALC 0 CELL PROG AUTO 2 000 REF MODE OFF A n UVCALC PRINT SAVE VIEW VIEW N RESULTS METHOD METHOD GRAPH RESULTS View G raph MANIPULATE page 19 01 98 SCAN GRAPH 0 281A A 11 35 ID TEST 1 550 0nm rr S 0 000 m 2 0007 Press Manipulate 400 0 4500 500 0 550 0 600 0 ABS MANIP PRINT SAVE SCAN VIEW MANIPULATE lt TTT ULATE GRAPH DATA PAGE RESULTS TRACK RESCAL COMPARE MODE Choose Track to report PEAKS SMOOTHING wavelength values ORIGINAL N 19 01 98 TRACK 0 281A 0 00 K S 11 35 ID TEST1 550 0nm 400 0 450 0 500 0 550 0 600 0 MANIP PRINT SAVE SCAN VIEW 2 000 ULATE GRAPH DATA PAGE RESULTS s ABS A 3 Choose Peaks to give 2 Peak Table 4 19 01 98 TRACK LIST 0 281A 4 11 35 D SNT 550 0nm K 0 000 MARK ABS A WAVELENGTH nm 400 0 480 0 500 0 550 0 600 0 SCAN PRINT CLEAR past VIEW 1 0 472 447 0 GRAPH GRAPH ALL TABLE 2 2 002 463 0 3 0 375 480 0 4 0 406 526 0 s 2 020 5450 Choose View Table to 6 0 441 559 0 see Track Table
2. 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 432 1nm PRINTERS PRINTER TYPE HP MONO PRINTER EPSON 9 PIN HP LASERJET HP MONO HP PAINTJET HP PLOTTER HP 690C HP 400 SETUP PAGE 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 432 1nm ENVIRONMENT LANGUAGE ENGLISH SOUND OFF DATE FORMAT dd MM yy AUTOMATIC CAL VAL OFF DEFAULT FILE TYPE NORMAL CVU WAVELENGTH TEST FILTER LIMS SUPPORT OFF USE SAMPLE IDS SEEDED SAMPLE ID SAMPLE ID SEED 5 AUTOSAVE RESULTS OFF AUTOPRINT RESULTS OFF USER LOG ON OFF HISTORY FILE ON SETUP UVCALC PAGE OFF 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 432 1nm WHITE LIGHT SETUP INITI PAGE ALISE UV Issue 2a 12 2000 19 01 98 UNICAM UV310 v5 0 11 35 CLOCK TIME DAY 25 MONTH 03 YEAR 98 66 55 T 432 1nm SETUP PAGE CANCEL ACCEPT 19 01 98 UNICAM UV510 v5 0 11 35 EHT CALIBRATION EHT TABLE NORMAL EHT TABLE NORMAL HIGH ABS FLAT SETUP CALIB PAGE RATE 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 432 1nm WAVELENGTH CALIBRATION CALIBRATION USING Hg LAMP CALIBRATION COMPLETED SETUP CALIBR PAGE RATE 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 4
3. UV Issue 2a 12 2000 13 SCAN RESCALE Q This option gives pop up menus for changing the graph x and y axis scales AUTO GRAPH HIGH Q Move the cursor to one of the options and press ENTER GRAPH LOW GRAPH START to select an operation GRAPH STOP PROCEED AUTO Displays the SCAN GRAPH with the x and y axes rescaled so that the Spectrum fills the screen GRAPH HIGH Pops up a window for entry of the GRAPH HIGH limit GRAPH LOW Pops up a window for entry of the GRAPH LOW limit GRAPH START Pops up a window for entry of the required start wavelength GRAPH STOP Pops up a window for entry of the required stop wavelength PROCEED Used after GRAPH HIGH GRAPH LOW GRAPH START or GRAPH STOP to return to the SCAN GRAPH page with the graph rescaled using the new parameters COMPARE Q When selected Compare goes to the LIBRARY page and displays a list of scan data files Highlight the desired file using the Up Down Arrow keys and press LOAD The reference spectrum is displayed as a dotted trace Q Once loaded the reference spectrum will remain on the screen and will be printed with all subsequent scans until removed To remove the reference spectrum from the display select MANIPULATE ORIGINAL or load a new method MODE Select from ABS T 1D 2D 3D 4D ABS Selects Absorbance T Selects transmittance 1D Selects first derivative This records the first derivative of the Absorbance spectrum 2D Selects
4. AUTOPRINT RESULTS OFF USER LOG ON OFF i HISTORY FILE OFF I I AUTOMATIC CAL VAL Toggles between OFF and ON with Enter a When ON and the CVU Calibration Validation Unit is fitted the instrument automatically waits on start up for the warm up period 60 minutes and then performs the Wavelength Absorbance and Stray Light calibration tests See CVU Section Pressing Clear aborts the calibration DEFAULT FILE TYPE i Selects the default file type on the SAVE RENAME page a Available formats are NORMAL The native file type used by the Local Control software CSV Comma separated variable JCAMP DX JCAMP data exchange format Use up down arrow keys to highlight choice and press ENTER to confirm selection CVU WAVELENGTH TEST Toggles between Filter or Hg Selects the method used by the CVU where fitted to perform the Wavelength Accuracy Test LIMS SUPPORT Toggles between OFF and ON a When ON results methods and sample IDs when selected are exported automatically after each measurement to the central LIMS computer via the RS232 port THIS INTERFACE MUST BE CONNECTED BEFORE LIMS SUPPORT IS ACTIVATED USE SAMPLE IDS Choose between OFF SEEDED and PROMPT USER Q OFF The system does not attach an identity to the sample ad SEEDED Enables the system to be set up to attach an identity to each sample automatically This appears on the screen and the print out It is also exported to the LIMS when
5. RATE VIEW ULATE DATA PAGE RESULTS 0 400 ibs MANIPULATE TRACK 0 200 SECTION RESCALE SMOOTHING ABS DISPLAY ORIGINAL ANOTHER CELL 19 01 98 RATE RESULTS 0 281A 0 000 i 550 0nm ID TEST 1 E 00 00 00 15 00 30 MANIP PRINT SAVE RATE VIEW Initial Abs 1 281 ULATE DATA PAGE RESULTS Final abs 0 567 Initial Time 00 00 Final Time 00 30 AA Min 1 281 Activity 4281 Corr Coeff 0 987 Smoothing LOW 19 01 98 TRACK 0 281A 11 35 ID TEST 1 550 0nm LIMS SAVE RATE VIEW PRINT DATA ane EXPORT PAGE GRAPH ABS 0 200 0 000 N A 00 00 00 15 00 30 19 01 98 RATE RESULTS 0 281A 11 35 TASTI 550 0nm RATE CLEAR past VIEW GRAPH ALL RESULTS 1 2 3 4 Initial Abs 1 281 1 281 1 281 1 281 Final abs 0 567 0 567 0 567 0567 Initial Time 00 00 00 00 00 00 00 00 Final Time 00 30 00 30 00 30 00 30 AA Min 1 281 1 281 1 281 1 281 Activity 4281 4281 4281 4281 Corr Coeff 0 987 0 987 0 987 0 987 Smoothing LOW LIMS PRINT SAVE RATE VIEW EXPORT DATA PAGE GRAPH 28 UV Issue 2a 12 2000 RATE a To select Rate highlight the RATE option on the HOME PAGE and press ENTER The RATE Methods page is displayed and from here the instrument and analysis parameters can be set up Q Move the cursor to the required parameter using the Up Down Arrow keys Press ENTER to enable a parameter to be changed Q Once the method has been set up press RUN The spectrophotometer will perform the rate and display the result on the
6. or edited and press ENTER to display the edit box Use the numeric 18 UV Issue 2a 12 2000 FIXED keypad to enter the wavelength and press ENTER when finished The instrument returns to the MULTI screen with the next wavelength in the list highlighted Up to 20 wavelengths may be entered When the list is finished press the ACCEPT function key to accept the new list or the CANCEL function key to return to the FIXED METHOD page without changing the wavelength list SERIAL Press ENTER to display the edit box for the wavelength to be used for the first sample Data entry is as for MULTI above When the required wavelengths have been entered press ACCEPT to accept the new list or press CANCEL to return to the FIXED METHOD page leaving the original list unchanged Available wavelength ranges depend on the instrument and are as follows Unicam UV510 520 530 540 190 0nm to 900 0nm Unicam UV310 320 330 340 190 0nm to 1100 0nm Unicam UV550 190 0nm to 850 0nm If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH N Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 Fixed at 1 5nm Unicam UV310 320 330 340 Select from 4 2 1 and 0 5nm Unicam UV550 Select from 4 2 1 1 5 1 5 1 0 5 and 0 2nm INTEGRATION Enter integration time in seconds a This sets the integration time for which the result
7. 0 2 0 1 120 4 2 1 0 5 0 2 0 1 30 4 2 1 0 5 0 2 0 1 10 4 2 1 0 5 0 2 0 1 1 4 2 1 0 5 0 2 0 1 The current spectrum will be lost if the scan speed is changed PEAK TABLE Select from OFF PEAKS VALLEYS PKS amp VALLEYS ZERO CROSS TRACK RATIO CORR RATIO PEAK HEIGHT Note RATIO CORR RATIO and PEAK HEIGHT do not appear on this menu when UVCalc has been installed UVCalc can perform these calculations if required in addition to a wide range of additional data manipulations See UVCalc Manual This selects the type of peak picking done automatically as part of the method Results are reported on the Peaks Page Peaks information is stored with any saved spectrum OFF PEAKS VALLEYS PKS amp VALLEYS ZERO CROSS TRACK RATIO CORR RATIO 10 Sets Peak Table to Off No peaks information is produced as part of the scan Picks the highest peaks in a spectrum up to a maximum of 10 peaks Picks the lowest troughs in a spectrum up to a maximum of 10 troughs Picks the 5 highest peaks and the 5 lowest valleys Picks all the points where the spectrum crosses zero up to a maximum of 10 crossing points This function allows the Absorbance or other mode values to be reported at up to 10 user selected wavelengths To enter the desired wavelengths select PEAK TABLE TRACK then press VIEW GRAPH You do not have to have a spectrum present on the graph to enter the selected wavelengths Pr
8. SCAN PRINT LIMS VIEW TRACK TABLE page GRAPH LIST EXPORT GRAPH 19 01 98 PEAK TABLE 0 281A 11 35 a men 550 0nm PEAK TABLE page ABS A WAVELENGTH nm 1 PEAK 0 472 447 0 2 PEAK 2 002 463 0 3 PEAK 0 375 480 0 4 PEAK 0 406 526 0 gt 5 PEAK 2 020 543 0 6 PEAK 0 441 559 0 SCAN PRINT LIMS GRAPH LIST EXPORT SCAN ad To select Scan highlight the SCAN option on the HOME PAGE and press ENTER The SCAN Methods page is displayed and from here the instrument and analysis parameters can be set up Q Move the cursor to the required parameter using the Up Down Arrow keys Press ENTER to enable a parameter to be changed Q Once the method has been set up press ZERO BASE to perform a baseline scan with the current method see page 4 and then press RUN The spectrophotometer will perform the scan and display the result on the SCAN GRAPH page From here the spectrum can be manipulated and saved to Library or Disk See LIBRARY for explanation of file functions SCAN PARAMETERS Page Note The current spectrum will be lost if any of the method parameters except for the Operator name are changed i SCAN i SCAN TYPE INTELLISCAN I I I ID TEST 1 I MODE ABS START 400 0 nm 1 STOP 600 0 nm BANDWIDTH 2 0 nm SPEED NORMAL DATA INTERVAL 1 0 nm I PEAK TABLE OFF GRAPH HIGH 2 000 GRAPH LOW 0 000 LAMP CHANGE 825 nm I OPERATOR Name i I UVCALC 0 CELL PROG AUTO REF MO
9. ye 19 01 98 0 281A FIXED 11 85 NERT 550 0nm MODE ABS MODE ABS D f1X123 SELECT SINGLE D 11x123 From FIXED TPAVELENGBIa gt i 590 021 SELECT SINGLE BANDWIDTH SELECT WAVELENGTH S 550 0nm page choose INTEGRATION BANDWIDTH 2 0nm DELAY TIME SINGLE INTEGRATION ris LAMP CHANGE MULTI A DELAY TIME 0 00 select type PATON HU LAMP CHANGE 325nm CELL PROG OPERATOR Name REF MODE UVCALC 10 CELL PROG AUTO D REF MODE OFF From FIXED page _ METHOD METHOD RESULTS PRINT SAVE VIEW select Mode METHOD Single no UVCalc 19 01 98 0 281A 19 01 98 0 281A 11 35 FIXED 550 0nm 11 35 FIXED 550 0nm MODE ABS MODE ABS ID f1X123 ID f1X123 SELECT ULTI SELECT SINGLE WAVELENGTH S 550 0nm WAVELENGTH S 550 0nm BANDWIDTH BANDWIDTH INTEGRATION MODE INTEGRATION MODE DELAY TIME DELAY TIME LAMP CHANGE ABS LAMP CHANGE ApS OPERATOR AT OPERATOR AT CELL PROG CELL PROG CONC REF MODE PRINT SAVE VIEW PRINT o METHOD ABS Or OT METHOD METHOD RESULTS 19 01 98 78 96 T z 19 01 9 0 281A 11 95 don ee iias d SAAN MODE 1 T MODE CONC ID f1X128 A SELECT MULTI D sos WAVELENGTH S 550 0nm Members Seon BANDWIDTH 2 0nm ja Li Erian a INTEGRATION 11s BAND SAR INTEGRATION 1s DELAY TIME 0 00 DELAY THE aoi LAMP CHANGE 325nm LAMP CHANGE 325nm OPERATOR Name OPERATOR aa VVCALC 2 FACTOR 1 0000 CELL PROG AUTO NTS ien REF MODE OFF CELL PROG AUTO REF MODE OFF PRINT SAVE VIEW
10. 09 03 98 0 281A 11 35 550 0nm ABS ABS 1 A1 2 036 12 1 646 COMPONENT CONC COMPONENT conc Holmium 0 9983 Didymium 0 9903 GOODNESS OF FIT 0 0000 PRINT SAVE MCA CLEAR LIST DATA PAGE RESULTS 37 MCA Q The MCA Multi Component Analysis application is able to measure up to 20 components in a mixture measuring up to 20 wavelengths per sample Q Standards can be measured at run time or loaded from files obtained using the MULTI 1 function of FIXED MCA METHOD Page MCA ID TEST 1 MEASURE STDS YES STANDARDS o UNITS I I I I WAVELENGTH S 1 BANDWIDTH 2 0nm INTEGRATION 1s DELAY TIME 00 H LAMP CHANGE 325nm OPERATOR Name CELL PROG AUTO REF MODE ______ OFF ID Enter a description using the TEXT ENTRY screen ad The ID identifies the method and will be saved with the method and any spectra produced by the method MEASURE STDS Use ENTER to toggle between YES and NO YES Standards are measured with the run and all fields remain editable NO Standards are loaded from Library or Disk The Wavelength s Bandwidth Integration Delay time Lamp change and Operator Name are also loaded with the standards and cannot be edited Any attempt to do so causes the prompt Change method by loading MCA standards to appear and no action is taken Changing the MEASURE STANDARDS parameter will cause all previous data to be lost STANDARDS Switches to the STANDARDS ENTRY screen Q Iden
11. N B If user log on is in operation the operator name cannot be changed UVCALC 4 Switches to the UVCALC screen if installed CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer reference mode if fitted FIXED Method Page Function Keys FIXED VIEW RESULTS Switches to the FIXED RESULTS page SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk PRINT METHOD Prints the current method parameters on the printer Q Pressing RUN starts a fixed measurement using the current method and switches to the FIXED RESULTS page Q Pressing ZERO starts a zero using the current method Any changes to the WAVELENGTH BANDWIDTH INTEGRATION or LAMP CHANGE parameters will invalidate the current results If AUTOPRINTING is selected see SETUP for details a change to the MODE parameter will invalidate the current results 20 UV Issue 2a 12 2000 FIXED FIXED RESULTS Page Q The layout of the page depends on the Mode and Select in use SINGLE In ABS or T modes up to 2 columns of results are displayed per page In CONC mode a single column of results is displayed on each page Results accumulate on the same page until it is full MULTI Two columns of results are displayed per page Results of each sample always start on a new page SERIAL One column of results is displayed per page Results accumulate on the same pag
12. PRINT SAVE VIEW METHOD METHOD RESULTS METHOD METHOD RESULTS or T measurements set parameters and press Run For Concentration measurements enter Factor and Units set parameters and press Run 19 01 98 39 6991 19 01 98 posta 19 01 98 0 281A x i On ml E p FIXED RESULTS FIXED RESULTS 11 35 FIXED RESULTS 550 0nm T T WAVELENGTH nm ABS ABS conc g 1 1 A 183 63 2 90 35 1 41 550 0 0 078 1 0 281 0 2108 4390 39 4 90 33 2 42 560 0 0 044 2 0 281 0 2108 5 90 30 6 89 73 3 43 570 0 0 044 3 0 281 0 2108 4 24 575 0 0 043 4 0 281 0 2108 5 A5 580 0 0 044 5 0 281 0 2108 6 16 454 0 0 644 7 17 536 0 0 248 8 1 8 420 0 0 152 9 19 410 0 0 057 LIMS PRINT SAVE FIXED CLEAR LIMS PRINT SAVE FIXED CLEAR LIMS PRINT SAVE FIXED CLEAR EXPORT LIST DATA PAGE RESULTS EXPORT LIST DATA PAGE RESULTS EXPORT LIST DATA PAGE RESULTS UV Issue 2a 12 2000 17 FIXED ad Instrument and analysis parameters are set up on the FIXED page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q Once the method has been set up press ZERO BASE to zero the instrument for the current method See page 4 and press RUN The spectrophotometer will perform a measurement and display the result on the FIXED RESULTS page Q Once all results have been collected sa
13. instrument An entry is put into the file when there are changes to default baselines wavelength calibrations EHT calibrations sipper calibrations and CVU tests noting date time and user The file will also contain records of operations carried out by engineers during maintenance visits When HISTORY FILE is set to ON the HISTORY FILE function key becomes available on the Environment Page unless USER LOG ON is enabled and the user has been denied access by the Administrator Pressing this function key brings up the History File Pop up box SAVE HISTORY ON DISK The user is prompted for a HISTORY FILE file name and the instrument history is saved in CSV format which may be read by a suitable spreadsheet or SAVE HISTORY ON DISK text editor CLEAR HISTORY PRINT HISTORY CLEAR HISTORY Clears the Instrument History PRINT HISTORY The Instrument History is printed out UV Issue 2a 12 2000 53 SETUP Make sure the printer is connected and ready before selecting PRINT HISTORY The History File contains a maximum of 400 entries When the number of entries reaches 390 a warning message is displayed and it is necessary for the Administrator or a user with the History File privilege save to disk and or print the existing history file then clear the history file to make room for more entries ENVIRONMENT DIVIPIG O HISTORY FILE Appears when History File is enabled CHANGE USERS Available only to Administrator w
14. is measured The current data will be lost if the integration time is changed DELAY TIME Set a time in the range 00 05 to 99 59 using to separate minutes and seconds a This sets the time between pressing RUN and the start of the measurement The range is from 0 to 99 minutes and 59 seconds LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE FACTOR Enter the factor for the concentration Only available in CONC mode the factor is used to multiply the absorbance result to produce a concentration result Changing the factor will not cause current results to be lost They will be recalculated using the new factor CONC mode is not available when UVCalc has been installed or when MULTI A or SERIAL X is selected UNITS Enter units for concentration using the TEXT ENTRY page UV Issue 2a 12 2000 19 FIXED Only available in CONC mode used to enter the required description of the concentrations up to 11 alphanumeric characters OPERATOR Switches to the TEXT ENTRY screen a The operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost
15. last calibration has been done put the first sample to be analysed in the beam and press RUN 42 UV Issue 2 09 99 MCA ANALYSING A SAMPLE When a calibration has been performed or loaded with the method the MCA application is ready to use When RUN is pressed from the METHOD or RESULTS pages the instrument will measure the absorbance of the sample at each of the wavelengths specified in the method and compare with the absorbances of the standards at these wavelengths The concentrations of each component are calculated and displayed on the results page Results for each sample are displayed on a new page Use the up and down arrow keys to move through the pages of results olll CLEAR RESULTS Clears the results table MCA PAGE Returns to the MCA METHOD page SAVE DATA Displays the SAVE page from which the results can be saved to Library or Disk PRINT LIST Prints the list on the selected printer UV Issue 2a 12 2000 43 SETUP SETUP UNICAM UV310 v5 0 SERIAL No 100661 19 01 9 11 35 HOME SCAN FIXED OUANT RATE MCA LIBRARY INSTRUMENT HOURS 12345 66 55 432 1nm CAL ACCESS SETUP VAL ORIES REMOTE 19 01 98 UNICAM UV510 v5 0 11 35 SETUP CLOCK EHT PRINTER ENVIRONMENT WAVELENGTH INITIALISE WHITE LIGHT 66 55 T 432 1nm
16. print using an external printer at any point during operation of the instrument ensure that the printer is ready to print Failure to do so will result in an error condition Press CLEAR to clear the error message Then rectify the problem with the printer and try again ENVIRONMENT page This page is used to select the language used for the software the use of the beep date format and to enable disable Automatic Calibration Validation and LIMS Laboratory Information Management System Support to select the default filetype used when saving results and to select whether wavelength calibration validation is carried out using the mergcury lamp or glass filters in the CVU with the tungsten lamp Where installed UVCalc can be turned off from the Environment Page by pressing the UVCALC OFF function key When UVCalc has been turned off the UVCalc disk will be required to reinstate it 50 UV Issue 2a 12 2000 1 LANGUAGE Select from the list ENVIRONMENT The language used immediately changes to the one selected i LANGUAGE ENGLISH SOUND Turns the warning i SOUND OFF i beeper ON or OFF If set to OFF then DATE FORMAT dd MM yy the only indication of any error is the AUTOMATIC CAL VAL OFF screen message DEFAULT FILE TYPE NORMAL i DATE FORMAT Defaults to CVU WAVELENGTH TEST FILTER dd MM yy but will toggle with ENTER to LIMS SUPPORT OFF i MM dd yy USE SAMPLE IDS OFF AUTOSAVE RESULTS OFF
17. results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page RATIO TABLE Page RATIO TABLE MARK ABS A WAVELENGTH nm Q The page shows the positions and values of the wavelengths and the ratio as selected by the RATIO or 2 2 002 463 0 CORR RATIO functions Not available if UVCalc has been installed VIEW GRAPH Returns to the SCAN GRAPH page LIMS EXPORT Sends the results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page PEAK HEIGHT Page PEAK HEIGHT a This page shows the position and values of the AA SAVELENOTH nen wavelengths and the peak height as selected by the 0 472 srt PEAK HEIGHT function Not available if UVCalc has 0 375 480 0 been installed VIEW GRAPH Returns to the SCAN GRAPH page LIMS EXPORT Sends results via the RS232 port PRINT LIST Prints the list on the selected printer SCAN GRAPH Returns to the SCAN GRAPH page 16 UV Issue 2a 12 2000 FIXED FIXED Page FIXED 19 01 98 11 35
18. tables first select the table to be calibrated and then press CALIBRATE CAUTION DO NOT RECALIBRATE THE EHT TABLES UNLESS YOU ARE ABSOLUTELY SURE YOU NEED TO DO SO EHT CALIBRATION CALIBRATE Calibrates the selected EHT table SETUP PAGE Returns to the SETUP page UV Issue 2a 12 2000 49 SETUP PRINTERS Page Q This page sets the system to work with the selected printer THE PRINTER MUST BE FITTED WITH A SERIAL INTERFACE Q The printer type is always highlighted The default printer is HP Mono To choose a printer press ENTER to display the list of supported printers and select using cursor keys Press ENTER to confirm entry Printer options Supported Printers EPSON 9 PIN Epson 9 or 24 Pin Dot Matrix EPSON 9 PIN using HP LASERJET ESC P language HP MONO HP LASERJET HP Laserjet Series HP PLOTTER HP MONO HP Deskjet 500 Series and HP 690C above Black amp White HP 400 HP PLOTTER Compatible with plotters using HPGL language HP 690C HP Deskjet 690C Colour HP 400 HP Deskjet 400 Mono Printers not on the above list that claim Epson 9 pin 24 pin ESC P or HP PCL Programming Control Language Level 3 compatibility should work with the instrument but are not guaranteed to do so and are therefore not supported If in doubt contact Thermo Spectronic approved Customer Support Note Printers designed to work only in a Windows environment are not compatible with Local Control Software Before attempting to
19. the Standard and press the ACCEPT function key when finished The instrument returns to the STANDARDS ENTRY page with the concentration field for the standard highlighted Press ENTER to display the edit dialogue box enter the concentration using the numeric keypad and press ENTER when finished The instrument returns to the STANDARDS ENTRY page with the ID for the next standard highlighted Up to 20 standards can be entered in this way The standards are displayed on two pages The STD11 STD20 function key toggles between the two pages When MEASURE STDS NO Press ENTER to display the LIBRARY page Select and load the files for each standard in turn A library of standards can be built up using the MULTI function in FIXED The same method must be used for each MULTI result and will be used for the MCA analysis When the first MULTI A file is loaded the current MCA method is changed to that used to obtain the MULTI A result Standards can thus be used in any combination without having to recalibrate for each new mixture UV Issue 2 09 99 MCA STD1 STD10 STD11 STD20 Toggles between displaying the first and second halves of the Standards List ACCEPT transfers to the MCA METHODS page with the new list accepted CANCEL Transfers to the MCA METHODS page leaving the old list unchanged WAVELENGTH ENTRY page Use the up and down arrow arrow keys to move up and down the list When the highlight is on the fiel
20. 2 2000 29 RATE da If the cell programmer is ON then the measurement time sets the time between individual measurements on the first cell i e the time for each cycle DELAY TIME Set a time in the range 00 05 to 99 59 This sets the time between pressing RUN and the start of the measurement The range is from 0 to 99 minutes and 59 seconds in steps of 1 second SLOPE N Select from POSITIVE or NEGATIVE Q Sets the graph to display positive or negative changes in Absorbance Choose POSITIVE if Absorbance increases with time Choose NEGATIVE if Absorbance decreases with time RANGE Set a number in the range 0 to 3A Q This sets the graph y axis Enter a number slightly larger than the change in Absorbance expected FACTOR Enter the factor for Activity as a number in the range 0 001 to 9999 999 UNITS Enters the units of Activity using the TEXT ENTRY page a Enters the required description or units of Activity up to 11 alphanumeric characters LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Q Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVERIT IS NOT IN USE OPERATOR Switches to the TEXT ENTRY screen a The operator name is automatically saved with the method and any data produced by the method Chan
21. 32 1nm INITIALISE INITIALISATION TYPE OPTICS INITIALISE WITH D2 OFF INITIALISE OPTICS BASELINE SETUP INITI PAGE ALISE 19 01 98 UNICAM UV310 v5 0 66 55 T 11 35 432 1nm LAMPS TUNGSTEN ON HOURS 54321 ENERGY 91 D2 OFF HOURS 1234 ENERGY 0 Hg OFF HOURS 2 ENERGY 0 SWITCH SWITCH RESET D2 Hg ENERGY HOURS 47 SETUP a This section describes how to set up the instrument Q The main system setup options are available directly from the SETUP function key on the HOME page SETUP Page Q From the SETUP page move the cursor to the required option using the Up Down Arrow keys Select the option by pressing the ENTER key WHITE LIGHT r 1 CLOCK SETUP EHT I I I I eae PRINTER PRINTER ENVIRONMENT ENVIRONMENT WAVELENGTH WAVELENGTH N INITIALISE I I I I WHITE LIGHT Page To return to the HOME page press HOME CLOCK Page CLOCK can be reset TIME HOURS 16 I I I I I I I I I I I I I I I I I I I I i i I I VINE se press ENTER i i I I I I I I i i i DATE I I I I DAY 25 MONTH 12 YEAR 96 unchanged OR TEEN RENNAIS N 48 Switches to the CLOCK page Switches to the EHT CALIBRATION page UV500 series spectrophotometers only Switches to the PRINTER page Switches to the ENVIRONMENT page Switches to the WAVELENGTH CALIBRATION page Switches to the OPTICAL
22. 4 0 300 65000 50 000 100 00 Place std 1 1 in beam and press run STOP Press RUN 19 01 98 QUANT PH 0 281A 11 35 ID UV123 550 0nm 3 000 ABS 2 000 1 000 0 300 l 0 0000 50 000 o g ml 100 00 COEFFICIENT 0 9999 SAVE PRINT STAND QUANT VIEW METHOD GRAPH ARDS PAGE RESULTS 19 01 98 0 281A 11 35 STAND s 550 0nm STD CONC ABSORBANCE 1 0 2500 0 1299 0 1299 2 0 5000 0 2179 0 2179 3 0 7500 0 4904 0 4904 4 1 0000 0 9312 0 9312 5 1 2500 1 4335 1 4335 6 1 5000 1 9332 1 9332 COEFFICIENT 0 9725 EQUATION OX 1 472X 0 425 EDIT EDIT QUANT VIEW VIEW CURVE STD PAGE RESULTS CALIB 22 STANDARDS page calibrated STANDARDS ENTRY page 19 01 98 11 35 STANDARDS 0 281A 550 0nm STD1 0 2500 STD11 STD2 STD3 VALUE STD4 STDS CONC 0 2500 STD6 STD7 STDS MINIMUM 0 0000 STD9 MAXIMUM 9999 9000 STD10 STD20 CANCEL ACCEPT QUANT RESULTS page 19 01 98 0 281A 11 35 QUANT RESULTS 550 0nm COEFFICIENT 0 5670 ABS CONC g ml 1 0 281 2 8100 2 0 281 2 8100 3 0 281 2 8100 4 0 281 2 8100 LIMS PRINT SAVE QUANT CLEAR EXPORT RESULTS DATA PAGE RESULTS Press Run to run samples UV Issue 2a 12 2000 QUANT Instrument and analysis parameters are set up on the QUANT page Move the cursor to the required parameter using the Up Down Arrow keys Change the parameter by pressing the ENTER key Q Once al
23. CROSS RATIO CORR RATIO PK HEIGHT Ratio Corr Ratio and Pk Height are not available when UVCalc has been installed SMOOTHING Applies LOW MEDIUM or HIGH modified improved Savitsky Golay smoothing to the spectrum ORIGINAL Resets the graph to display the data as originally collected TRACK Q To move the vertical cursor across the screen use the Left and Right Arrow keys The cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current wavelength Up to 10 wavelengths can be selected TRACK page function keys Q Pressing CLEAR will delete markers in turn highest number first Q The x axis values are listed on the TRACK table page ne abs Further markers can be added to the spectrum at any time however selecting TRACK will cause any previous PEAK TABLE information to be lost VIEW TABLE Switches to the TRACK TABLE page FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater The function key label shows the next speed ie the opposite to the one selected CLEAR ALL Clears all the markers and the TRACK TABLE PRINT GRAPH Provides a hardcopy of the results showing the markers and x and y axis values SCAN GRAPH Returns to the SCAN GRAPH page
24. DE i OFF J SCAN TYPE i Select from STANDARD INTELLISCAN Q Standard mode enables the setting of a given scan speed and data interval Intelliscan mode sets the data interval automatically and varies the scan speed according to the Absorbance of the spectrum ID Enter a description using the TEXT ENTRY screen ad The ID identifies the method and will be saved with the method and any spectra produced by the method MODE Select from ABS T 1 1D 2D 3D 4D ABS Selects Absorbance T Selects transmittance Selects Intensity mode This will measure the intensity of the signal in the sample beam Note If you are scanning in this mode on a 500 series instrument you should set the EHT table to FLAT 1D Selects first derivative This records the first derivative of the Absorbance spectrum 2D Selects second derivative This records the second derivative of the Absorbance spectrum 3D Selects third derivative This records the third derivative of the Absorbance spectrum 8 UV Issue 2a 12 2000 SCAN 4D Selects fourth derivative This records the fourth derivative of the Absorbance spectrum The current spectrum will be lost if the Scan Mode is changed START Selects start wavelength Unicam UV510 520 530 540 Between 190 0nm and 896 0nm Unicam UV310 320 330 340 Between 190 0nm and 1096 0nm Unicam UV550 Between 190 0nm and 846 0nm If the start wavelength requires the Deuterium lamp then this will be swit
25. Deuterium lamp during initialisation WHITE LIGHT Page Q The WHITE LIGHT feature is used to facilitate alignment of optical accessories in the sample compartment Q When the INITIALISE function key is pressed the instrument will align the grating so that the zero order diffraction passes through the sample compartment This provides a beam of white light which can be seen when a white card or similar is placed in the light path In double beam instruments the action of the chopper causes the light to alternate between the sample and reference beams Q When alignment is completed pressing the STOP function key returns the grating to its normal position and pressing the SETUP PAGE function key returns to the Setup Page LAMPS Page Q The lamp functions are available directly from the LAMPS key on the SETUP page If User Log on is not in use the HOME page will also have a LAMPS key ad This page shows the status of the Tungsten Halogen Deuterium and Mercury lamps whether ON OFF or FAILED and their appropriate energy levels It also allows the lamp hours to be reset and the Deuterium and Mercury lamps to be switched on or off THE MERCURY LAMP SHOULD BE SWITCHED OFF WHEN NOT IN USE Q The HOURS parameter states the number of hours that the lamp has been in use The Tungsten Halogen lamp should be replaced after 2000 hours The Deuterium lamp should be replaced after 1000 hours Whenever a lamp is changed then the hours parameter shou
26. HLB ADMIN ADMIN VEN SEA E EDIT METHODS H HISTORY FILE C CALIBRATIONS L RESET LIFETIMES F DELETE FILES B DEFAULT BASELINE I INITIALISATIONS USERS CANCEL ACCEPT spp Pressing the CHANGE USERS function key brings up the CURRENT USERS page Up to 20 users can be listed by name and password The privileges of each user can be set individually by the Administrator and can be any combination of Edit Methods Calibrations Delete Files Initialisations History File Reset Lifetimes Default Baseline Only the Administrator is able to change passwords or edit the Current Users Page It is strongly recommended that a new user name and password for the Administrator are set as soon as USER LOG ON is activated and that USER LOG ON is activated whenever the instrument is to be used in a multi user environment After USER LOG ON is enabled each time the instrument is powered up the user will be prompted to log on by entering name and password At the close of a session the user logs off by pressing the LOG OFF function key on the Home Page and choosing whether to PROCEED or STOP When PROCEED is chosen the system waits for the next user to enter their name USER LOG ON can be reset to OFF only by the Administrator After USER LOG ON has been set to OFF the list of users is cleared and the default User Name and Password are both reset to ADMIN HISTORY FILE Toggles between ON and OFF The History File contains the history of the
27. INITIALISATION page WHITE LIGHT Switches to the Q From this page the internal spectrophotometer clock calendar Q To reset the time or date highlight the required parameter and press ENTER Enter the new value using the number keys and a Once all the parameters have been changed press ACCEPT The date or time will not be changed unless ACCEPT is pressed Q CANCEL cancels the edit leaving the previous values UV Issue 2a 12 2000 SETUP EHT CALIBRATION Page EHT CALIBRATION EHT TABLE NORMAL EHT TABLE NORMAL HIGH ABS FLAT SETUP CALIB PAGE RATE Q The EHT function is not available on the Unicam UV300 series spectrophotometers a This page is used to select and calibrate the EHT table used The EHT table sets the amount of gain used at the photomultiplier detector ensuring that the response of the detector is kept optimal across the wavelength range If the EHT is set too low then the signal will be noisy conversely if the EHT is set too high then the detector will saturate a To select the EHT table press ENTER NORMAL This is the table for normal use HIGH ABS Use this table for highly attenuating accessories FLAT Use this table when scanning in intensity mode Normally the EHT table compensates for variations in the source intensity Consequently use of the NORMAL or HIGH ABS options will result in major loss of detail in the intensity spectrum Q To recalibrate any of the
28. OD setup and MANIPULATE functions are exactly the same except that the MEASURE TIME now sets the time between each cycle i e the length of time between measurements on the first cell The number of measurements taken on each cell is set by the CELL CYCLES parameter on the cell programmer page The total time over which the measurements are made is the result of the time between measurements MEASURE TIME and the number of measurements CELL CYCLES For example an analysis using 4 cells with MEASURE TIME set to 15 seconds and CELL CYCLES set to 20 would give a total measurement time of 5 minutes To set up a parallel rate method do the following a Set up the method as normal on the RATE PARAMETERS page Note that MEASURE TIME specifies the time between each measurement on the first cell a Go to the Cell Programmer page Press HOME then ACCESSORIES and select CELL PROG Set up the cell programmer method as required Return to the RATE PARAMETERS page press ZERO BASE to zero the instrument if reguired then press RUN The analysis will proceed with the rate graph for the first cell being drawn on screen To view the result of any other cell press MANIPULATE on the RATE GRAPH page and select ANOTHER CELL The cell number currently displayed is shown to the right of the ID line UV Issue 2a 12 2000 35 RATE Q To print the results press PRINT on either the RATE GRAPH page or the RATE RESULTS page Three further prin
29. Once all the required characters have been entered press ACCEPT Numbers are entered using the numeric keypad If you make a mistake ololoiole will act as a backspace or CLEAR will clear the whole entry Filenames are limited to 8 characters a To change the FILE TYPE highlight the field and press ENTER to display the pop up menu Available formats are NORMAL the native file type of the Local Control Software CSV Comma separated variable and JCAMP DX JCAMP data exchange format Highlight your choice and press ENTER to select The ID field will contain the characters entered for the method To edit the ID highlight the ID field and press ENTER change as necessary then press ACCEPT To select the destination of the file highlight the DRIVE field Pressing ENTER will toggle between LIBRARY and DISK SAVE loloj lt Acts as backspace and clears the last character in the line ACCEPT SAVE Accepts the edited field ready for saving Only reads ACCEPT if edit is in progress SAVE saves file to disk and is not present during an edit CANCEL Abandons the naming operation No files will be changed and the software returns to the calling screen UV Issue 2a 12 2000 SCAN SCAN page SCAN
30. RATE GRAPH page From here the data can be manipulated and saved to Disk RATE PARAMETERS Page Note The current data will be lost if any of the method parameters except for the ID Slope Factor Units and Operator name are changed RATE ID TEST 1 WAVELENGTH 340 0 nm BANDWIDTH 2 0 nm MEASURE TIME 00 30 DELAY TIME 00 00 SLOPE POSITIVE RANGE 0 5 FACTOR 1 000 UNITS 1 U LAMP CHANGE 325 nm OPERATOR Name CELL PROG AUTO REF MODE oi OFF ID Enter a description using the TEXT ENTRY screen ad The ID identifies the method and will be saved with the method and any results produced by the method WAVELENGTH Unicam UV510 520 530 540 Unicam UV310 320 330 340 Unicam UV550 Selection depends on the type of spectrophotometer 190 0nm to 900 0nm 190 0nm to 1100 0nm 190 0nm to 850 0nm a Ifa wavelength is selected that requires the Deuterium lamp then this will automatically be switched on Any current data will be lost if the wavelength is changed BANDWIDTH Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 Fixed at 1 5nm Unicam UV310 320 330 340 Select from 4 2 1 5 1 and 0 5nm Unicam UV550 Select from 4 2 1 5 1 0 5 and 0 2nm MEASURE TIME Set a time in the range 00 05 to 99 59 a This sets the time over which the sample will be measured The range is from 5 seconds to 99 minutes and 59 seconds in steps of 1 second UV Issue 2a 1
31. Track Table screen if track has been used VIEW GRAPH Switches to the Scan Graph screen SAVE METHOD Brings up the Filename Function screen and then saves the method including ID operator name and track wavelengths if the PEAK TABLE parameter is set to TRACK PRINT METHOD Prints the current method parameters on the printer UVCALC RESULTS Switches to UVCalc results screen if an equation has been entered and results are available Only available if UVCalc has been installed SCAN GRAPH Page a This page displays spectra and allows them to be manipulated 400 0 500 0 600 0 VIEW RESULTS Switches to the PEAK TABLE page SCAN PAGE Returns to the SCAN page SAVE DATA Displays the SAVE screen from where method and data can be saved to disk PRINT GRAPH Provides a hardcopy of the results as shown on screen MANIPULATE Displays the Manipulate options Q Pressing RUN starts a scan using the current method Q Pressing ZERO BASE starts a baseline using the current method See page 4 12 UV Issue 2a 12 2000 SCAN MANIPULATE OPTIONS TRACK Reports x and y axis values using the tracking cursor RESCALE Changes x and y axis scales automatically or manually SMOOTHING COMPARE Loads reference spectrum for comparison ORIGINAL MODE Changes mode Select from T ABS 1D 2D 3D 4D PEAKS Finds spectral peaks Select from PEAKS VALLEYS PEAKS amp VALLEYS ZERO
32. Unicam UV Series Local Control Software User Manual English 9499 230 43311 Issue 2a 001201 Copyright 2000 Unicam Limited Registration No 441506 All rights reserved Thermo Spectronic Europe Thermo Spectronic North America For Indian Sub continent Far East Middle East and Africa and Latin America and Australasia Mercers Row Cambridge CB5 8HY UK 820 Linden Avenue Rochester NY 14625 Contact Thermo Spectronic Europe Tel 44 0 1223 446600 USA Middle East and Africa Fax 44 0 1223 446644 Tel 800 654 9955 or 716 248 4000 E mail info thermospectronic co uk Fax 716 248 4014 www thermospectronic com E mail info thermospectronic com www thermospectronic com Thermo Spectronic A Thermo Electron business GENERAL GENERAL Introduction ad The range comprises a group of UV Visible spectrophotometers which can be controlled independently via an integral keypad and LCD display or externally from a computer The Unicam UV300 and 500 series include both local control and blind bench variants Q This manual describes the operation of the Local Control software on the Unicam UV310 UV510 UV530 and UV550 spectrophotometers Q The system is composed of a spectrophotometer with integral keypad LCD display 1 44 Mbyte Disk Drive Local Control Software and output device Q Always remove disks from the disk drive when not in use Never power up the instrument with a disk in place as permanent damage may be c
33. age STANDARDS Switches to the Quant Standards page PRINT GRAPH Prints the Quant method and calibration graph SAVE METHOD Switches to the SAVE page QUANT RESULTS Page a To view further pages of results use the Up and Down arrow keys Q Pressing RUN takes another sample measurement and displays the result Q Results are numbered sequentially and any batch can be of up to 600 samples QUANT RESULTS ABS CONC mg 1 1 0 201 10 122 2 0 201 10 122 CLEAR RESULTS Deletes all results from the results table QUANT PAGE Switches back to the Quant page SAVE DATA Brings up the SAVE page PRINT LIST Gives a printout of the results LIMS EXPORT Sends the results via the RS232 port UV Issue 2 09 99 27 RATE RATE 19 01 98 11 35 RATES ID WAVELENGTH 550 0nm BANDWIDTH 2 0nm MEASURE TIME 00 30 DELAY TIME 00 00 SLOPE POSITIVE RANGE 0 5 FACTOR 1 000 UNITS LAMP CHANGE en 19 01 98 RATE GRAPH 0 281A OPERATOR Name 11 35 ID TEST 1 550 0nm CELL PROG AUTO 0 400 PRINT SAVE VIEW VIEW METHOD METHOD GRAPH RESULTS Pa 0 200 Press Run 0 000 N i 00 00 00 15 00 30 x x 0 281A KI i hy DT TESTI So Onm MANE PRINT SAVE
34. ally after each run AUTOPRINT RESULTS Toggles between ON and OFF When ON results are printed automatically after each run Before attempting to print at any point during operation of the instrument ensure that the printer is ready to print ie switched on on line and supplied with paper Failure to do so will result in an error condition Press Clear to clear the error message the system may take a little time to respond Ensure the printer is ready and retry USER LOG ON The default setting is OFF Changing the setting to ON is password protected When set to OFF any user has full access to all the functions of the instrument When set to ON users must identify themselves by user name and password at log on and then have access to whichever functions are enabled for them by the System Administrator Setting User Log on to ON is password protected Use the up and down arrow keys to move the highlight to USER LOG ON and press Enter This brings up the Text Entry Page When the correct password is entered USER LOG ON is set to ON otherwise an error message is displayed and it remains set to OFF The default password is ADMIN Note that the password is case sensitive and in this password all the letters are upper case Logging on as ADMIN gives access to the system at Administrator level and the CHANGE USERS function key is enabled 52 UV Issue 2a 12 2000 SETUP CURRENT USERS NAME PASSWORD PRIVILEGES ECFI
35. aph At least one wavelength must be entered for each standard UV Issue 2a 12 2000 41 MCA oloo ACCEPT Transfers to the MCA METHODS page with all marked wavelengths added to the wavelength list FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater CLEAR ALL Clears all marks RESCALE Rescales the x and y axes so that the spectrum fills the screen CALIBRATION When the Standard Identifications and concentrations have been entered via the STANDARDS ENTRY page and the wavelengths have been entered via the WAVELENGTH ENTRY page the MCA application is ready for calibration Clear the beam s and press ZERO BASE to perform a baseline scan Press the CALIBRATE function key from the MCA METHODS page If any results are present you will be given the option to proceed or cancel with PROCEED highlighted All previous data will be lost if PROCEED is selected Press ENTER to proceed The message Press RUN to measure STD1 will be displayed Put the first standard in the beam then press RUN Continue until all standards have been measured Results for each standard will be stored in the Calibration Results Table with results for each standard on a new page Use the up and down arrow keys to move between standards When the
36. aused to the disk The only exception to this rule is when upgrading the instrument software and installing the UVCalc accessory Then automatic recognition of a software disk causes an automatic upgrade of the software Q The Local Control software controls all aspects of the systems operation Q Where installed the UVCalc software accessory provides automatic calculation of results from measurements using user defined equations in SCAN FIXED and QUANT modes I DEUTERIUM LAMP TUNGSTEN LAMP REFERENCE BEAM POWER ON OFF SAMPLE BEAM MAINS DISK DRIVE RS 232 FOR COMPUTER PRINTER SUPERSIPPER sue aa KEYPAD COMPARTMENT CONTRAST END PLATE CONTROL LCD UNDER LEDs CELL PROGRAMMER UV Issue 2a 12 2000 3 GENERAL User Interface lt lt NUMERICAL KEYS LCD DISPLAY ARROW KEYS FUNCTION KEYS ON CLEAR ZERO BASE ENTER GREEN KEY KEY KEY STANDBY HOME RUN RED KEY KEY Key Operation Arrow Keys Highlight menu options or move track cursor or move Cell Programmer depending on page in use Change display contrast with lt gt from Home Page Numerical Keys Enter numbers minus and decimal point Function Keys Access and perform system functions Operation depends on screen in use and is indicated by labels at bottom of screen Clear Clears entry leaving field or parameter ready for new entry clears pop up and clears error messages Enter Enters changes to field or paramete
37. beams are clear From WAVELENGTH CALIBRATION page press CALIBRATE Once calibration is complete switch the instrument OFF Switch instrument and Deuterium lamp ON and allow to warm up Run Default Baseline as detail below Oooooo OPTICAL INITIALISATION Page Q This page is used to reset the instrument and define its initialisation and default baseline These procedures ensure the optimum performance of the spectrophotometer INITIALISATION TYPE When selected this displays a pop up menu to choose between initialisation of optics or baseline OPTICS During the normal initialisation procedure the spectrophotometer optimises the alignment of the lamp change mirror over a predefined range to give the maximum energy throughput this operation defines the range over which the optimisation takes place This should always be done when one of the lamps is changed BASELINE This re measures the default baseline Ensure that both lamps are on and that the spectrophotometer is fully warmed up This process will take about one hour UV Issue 2a 12 2000 55 SETUP The default baseline should be re measured whenever one of the source lamps is changed or if the instrument is working at temperatures significantly different from 25 C or if the wavelength calibration is altered INITIALISE WITH D2 This sets the instrument to initialise with or without the Deuterium lamp on If set to ON then the instrument will automatically strike the
38. ce between the new start and stop times Results are listed on the RATE RESULTS page Up to four discrete pairs of cursors can be placed on the graph Arrows are placed on the cursors and results are displayed on the RATE RESULTS page for those parts of the graph indicated by the arrows Q Selecting SECTION will remove the TRACK markers Q The minimum interval between TRACK cursors is one second 32 UV Issue 2a 12 2000 RATE VIEW RESULTS Switches to the RATE RESULTS page FAST SLOW Toggles between two cursor speeds In FAST mode the cursor jumps 5 of the graph or to the next data point whichever is the greater In SLOW mode the cursor jumps to the next data point or the next display pixel whichever is the greater The function key label shows the next speed ie the opposite to the one selected CLEAR ALL Clears all the markers RATE GRAPH Returns to the RATE GRAPH page SECTION Q To move the vertical cursor across the screen use the Left and Right Arrow keys The cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current time ad To delete a marker place the cursor on the marker and press CLEAR Up to five markers can be placed on the graph Rate results will be reported between markers providing a maximum of four sets of results Sections The minimum Section size is one second Results are listed on the RATE RESULTS page Selecting TRACK will remove t
39. ched on The Start wavelength must be at least 4 nm less than Stop wavelength The current spectrum will be lost if the Start wavelength is changed STOP Selects stop wavelength Unicam UV510 520 530 540 Between 194 0nm and 900 0nm Unicam UV310 320 330 340 Between 194 0nm and 1100 0nm Unicam UV550 Between 194 0nm and 850 0nm The Stop wavelength must be at least 4 nm greater than the Start wavelength The current spectrum will be lost if the Stop wavelength is changed BANDWIDTH Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 Fixed at 1 5nm Unicam UV310 320 330 340 Select from 4 2 1 5 1 and 0 5nm Unicam UV550 Select from 4 2 1 5 1 0 5 and 0 2nm SPEED Selection depends on Scan Type selected a In Intelliscan mode select from ZIP SURVEY NORMAL QUANT HIGH RES VERY HIGH RES Q In Standard mode select from 3800 2400 1200 600 240 120 30 10 or 1 nm per min DATA INTERVAL Sets the frequency of data points in the spectrum Selection depends on Scan Type In Intelliscan mode the data interval is fixed according to the intelliscan type selected Intelliscan Interval between points ZIP 4nm SURVEY 2nm NORMAL 1nm QUANT 0 5 nm HIGH RES 0 2 nm VERY HIGH RES 0 1 nm UV Issue 2a 12 2000 9 SCAN In Standard mode the choice of data interval depends on the scan speed selected Speeds Data Intervals 3800 4 2400 4 2 1200 4 2 1 600 4 2 1 0 5 240 4 2 1 0 5
40. d to be entered or edited press ENTER to display the EDIT box and enter the values with the numeric keys It is not necessary to enter the values in numerical order although analysis will be quicker if they are Press ENTER when finished The instrument will return to the WAVELENGTH ENTRY page with the new value inserted in the list and the highlight moved to the next wavelength When all wavelengths have been entered press the ACCEPT function key to return to the MCA methods page using the new list or the CANCEL function key to return leaving the old list unchanged Alternatively wavelengths may be entered directly from a scan Clear the beam s and press ZERO BASE to perform a baseline scan then put the sample in the cell holder and press the SCAN GRAPH function key The instrument performs a scan using the method currently entered in the SCAN PARAMETERS page Use left and right arrow keys to move the vertical cursor to a suitable wavelength and press ENTER to mark it Repeat until all required wavelengths have been marked Marks can be removed using the CLEAR key or the CLEAR ALL function key When all required wavelengths have been marked press the ACCEPT function key to accept the list and return to the MCA Methods page Wavelengths can be selected either from a scan of the mixture to be analysed or by performing a scan on each standard in turn and selecting suitable wavelengths Wavelengths already entered in the table are shown on the Scan Gr
41. e until it is full Q To move up or down pages of results use the Up Down arrow keys Q Results are numbered sequentially from 1 to 600 Conc values are from 0 0001 to 9999 9 Any concentration value outside this range is marked UNDER RANGE or OVER RANGE FIXED RESULTS ABS ABS 1 0 201 15 2 0 201 16 CLEAR RESULTS All results are cleared ready for the start of the next batch FIXED PAGE Returns to the FIXED METHOD page SAVE DATA Brings up the SAVE page from where the results can be saved to Disk PRINT LIST Prints the list on the selected printer LIMS EXPORT Sends the results via the RS232 port a Press RUN to take another sample measurement Q Press ZERO BASE to zero the instrument at the wavelength s specified in the method See page 4 UV Issue 2a 12 2000 21 QUANT QUANT page QUANT Set parameters as required eae Fill in standards _ 19 01 98 0 281A 11 35 QUANT 550 0nm ID WAVELENGTH 550 0nm BANDWIDTH 2 0nm INTEGRATION 1s STANDARDS 3 REPLICATES 21 UNITS CURVE FIT LINEAR LAMP CHANGE 325nm OPERATOR Name UVCALC 0 MEASURE STDS YES CELL PROG AUTO CALIB PRINT SAVE VIEW VIEW RATE METHOD METHOD RESULTS CALIB Calibrate method 19 01 98 QUANT GRAPH 0 281A 11 35 ID 550 0nm 3 000 ABS 2 000 1 000 E L F
42. enabled with the results of the run and method used Selection of SEEDED causes two additional items to appear in the Environment Menu UV Issue 2a 12 2000 51 SETUP SAMPLE ID Enter the name of the sample via the Text Entry Page Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters up to 11 have been entered press ACCEPT If you make a mistake will act as a backspace or CLEAR will clear the whole entry SAMPLE ID SEED Sets the number to be used for the first sample via the numeric keypad The Sample ID is incremented automatically before each run Set the seed to zero for the results of the first run to be numbered 1 Q PROMPT USER Before each run the Text Entry Page appears and the user is prompted to enter an identity for the sample NOTE a When the Cell Programmer is used in AUTO mode the Sample ID is incremented automatically without stopping for ID confirmation between samples b PROMPT USER is not compatible with the Sipper used in Sip and Run or AutoSampler modes AUTOSAVE RESULTS Toggles between ON and OFF When ON results are saved automatically after each run Selecting ON causes two additional items to appear in the Environment menu FILENAME Enter a filename of up to 5 characters via the Text Entry Page FILE NUMBER Enter a number between 0 and 999 via the numeric keypad The number is appended to the filename and incremented automatic
43. ess MANIPULATE and select TRACK For each wavelength move the cursor to the desired position press ENTER Once all the wavelengths have been entered go back to the SCAN METHOD page and save the method This function allows the ratio 44 4 to be automatically calculated at the end of the scan To enter the desired wavelengths select PEAK TABLE and press ENTER then select RATIO A pop up box appears in which to enter the first wavelength Enter the desired wavelength and press ENTER Repeat for the second wavelength Once all the method parameters have been set save the method This function allows the ratio of two wavelengths to be calculated relative to a third wavelength A A3 A A3 automatically at the end UV Issue 2a 12 2000 PEAK HEIGHT GRAPH HIGH GRAPH LOW LAMP CHANGE SCAN of ascan To enter the desired wavelengths select PEAK TABLE and press ENTER then select CORR RATIO A pop up box appears in which to enter the first wavelength Enter the desired wavelength and press ENTER Repeat for the second and correction wavelengths Once all the method parameters have been set save the method This function allows the height of a peak to be calculated relative to a local baseline rather than y 0 To enter the desired wavelengths select PEAK TABLE and press ENTER then select PEAK HEIGHT A pop up box appears in which to enter the wavelengths required and define the baseline defines the peak Once all the
44. ging the operator name will not cause the current data to be lost If User Log on is in operation the operator name cannot be changed CELL PROG Switches to the CELL PROG screen if fitted REF MODE l Toggles the status of the Cell Programmer reference mode if fitted 30 UV Issue 2a 12 2000 RATE RATE PARAMETERS Page function keys RATE olol E VIEW RESULTS Goes to the RATE RESULTS page VIEW GRAPH Goes to the RATE GRAPH page SAVE METHOD Brings up the SAVE page from where the method can be saved to Library or Disk PRINT METHOD Prints the current method parameters on the printer RATE GRAPH Page Q This page displays the RATE curve and allows it to be manipulated VIEW RESULTS Switches to the RATE RESULTS page RATE PAGE Returns to the RATE PARAMETERS page SAVE DATA Brings up the SAVE screen from where method and data can be saved to disk PRINT Provides a hardcopy of the results i e RATE GRAPH and RATE RESULTS If a Cell Programmer is fitted see the note below MANIPULATE Brings up the Manipulate options ad If more than one rate has been run in parallel using the Cell Programmer then three further print options are available ALL OVERLAY ALL SEQUENTIAL and ONE RESULT ALL OVERLAY Prints the results of all cells in the run on one sheet of paper up to a maximum of 4 results If more than four results are present then the remainder are
45. h at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE The current data will be lost if the lamp change parameters are changed OPERATOR Switches to the TEXT ENTRY screen a The operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost If User Log on is in operation the operator name cannot be changed CELL PROG Switches to the CELL PROG screen if fitted REF MODE Toggles the status of the Cell Programmer Reference Mode if fitted UV Issue 2a 12 2000 39 MCA VIEW RESULTS Transfers to the Results Table if it contains results VIEW STDS Transfers to Standards results table if a calibration has been performed SAVE METHOD Brings up the SAVE page from where the method can be saved to Disk PRINT METHOD Prints the current method parameters on the printer CALIBRATE Moves to the CALIBRATION PAGE to perform a calibration if standards and wavelengths have been entered STANDARDS ENTRY Page Q When MEASURE STDS YES Use the up and down arrow keys to move up and down the list When the highlight is on the field to be entered or edited press ENTER to display the Text Entry dialogue box Enter the name of
46. he method can be saved to Library or Disk PRINT METHOD Prints the current method parameters and the standards table on the printer CALIBRATE Switches to the Standards Entry page QUANT STANDARDS Page Q This page lists the standards as defined in the QUANT METHOD Before the system can be calibrated each standard must have a concentration entered Q To enter Standard concentrations use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display the Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry Page with the highlight on the next standard on the list Up to 20 standards can be specified When all the standards have been entered press the ACCEPT function key to return to the QUANT Page with the new list of standards or CANCEL to return leaving the old list unchanged a Ifa calibration has been done then the correlation coefficient and the equation are displayed a Ifa calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and switches to the Quant Results screen Pressing ZERO BASE starts a zero using the current method UV Issue 2 09 99 25 QUANT STANDARDS VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration ex
47. he Edit pop up Use the numeric keys to enter the concentration of the standard and press ENTER when finished The instrument returns to the Standards Entry page with the highlight on the next standard on the list Up to 20 standards can be specified Changing the standards will cause any current data to be lost UV Issue 2 09 99 23 QUANT REPLICATES Enter number of replicates for each standard Q Sets the number of times each standard is measured maximum 3 Each value obtained is used in the calibration UNITS Enter units for concentration using the TEXT ENTRY page CURVE FIT Select from LINEAR LINEAR TO 0 QUADRATIC QUAD TO 0 Q Selects the curve fit algorithm used in the calibration LINEAR performs a linear calibration At least two standards are required LINEAR TO O performs a linear calibration forced through zero QUADRATIC performs a quadratic fit on the data At least three standards are required QUAD TO 0 performs a quadratic fit with the data forced through zero At least two standards are required Changing the curve fit will cause the existing calibration to be recalculated Any results associated with the previous calibration will be lost LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the
48. he SECTION markers RESCALE a This option gives a pop up menu for changing the graph y axis scale Q Move the cursor to one of the options and press ENTER to select an operation The rescale options depend on whether Absolute or Relative Absorbance has been selected Absolute Absorbance AUTO Displays the RATE GRAPH with the y axis rescaled so that the trace fills the screen AUTO GRAPH HIGH GRAPH HIGH GRAPH LOW Allow the user to set the RAF LOW upper and lower limits for the RATE GRAPH y axis UV Issue 2a 12 2000 33 RATE Relative Absorbance RESCALE AUTO Displays the RATE GRAPH with the y axis rescaled so that the trace fills the screen AUTO RANGE RANGE Allows the user to set the upper y axis value ABS DISPLAY a This option enables the RATE GRAPH to be redisplayed with Absolute Absorbance or Relative Absorbance values SMOOTHING a This option offers a pop up menu to choose one of three smoothing algorithms Choices are NONE LOW MEDIUM and HIGH A moving point average is performed on the data and in each case data points will be lost from either end of the data SMOOTHING No of POINTS POINTS LOST AT USED EACH END NONE 0 0 LOW 9 4 MEDIUM 17 8 HIGH 37 18 ORIGINAL This removes any manipulation and displays the rate graph as originally specified by the rate method ANOTHER CELL Q If more than one rate has been run in parallel using the Cell Programmer then this function allows
49. hen User Log on enabled UVCALC OFF Only appears when UVCalc has been installed Disables UVCalc and re enables functions that UVCalc disables SETUP PAGE Returns to the SETUP page WAVELENGTH CALIBRATION Page Q From this page the Wavelength calibration of the instrument can be adjusted CALIBRATION Select between USING HG LAMP and USING D2 LAMP WARNING DO NOT ATTEMPT TO RECALIBRATE THE INSTRUMENT UNLESS YOU ARE ABSOLUTELY SURE YOU NEED TO DO SO IF IN ANY DOUBT CONTACT THERMO SPECTRONIC APPROVED CUSTOMER SUPPORT To find out whether recalibration is needed it will be necessary to check the Wavelength Accuracy This is most conveniently done with the optional CVU and Self Test Accessories Performing a Recalibration ENSURE BOTH SAMPLE AND REFERENCE BEAMS ARE CLEAR BEFORE ATTEMPTING ANY WAVELENGTH CALIBRATION a There are two options calibration with the optional Mercury lamp or recalibration using the Deuterium lamp Pressing ENTER toggles between them Q Press the SETUP PAGE function key to return to the SETUP page Q Press the CALIBRATE function key to begin calibration STOP becomes available during the calibration process It stops the calibration process and leaves the existing calibration unchanged 54 UV Issue 2a 12 2000 SETUP Mercury Lamp Calibration a This option will optimise the wavelength calibration of the instrument by measuring the position of the emission lines of the mercury lamp and fitting
50. ists VIEW RESULTS Switches to the QUANT RESULTS page if there are any results in the table QUANT PAGE Switches back to the Quant page EDIT STD Available after calibration Allows each standard to be used ignored or remeasured EDIT CURVE Available after calibration Allows curve fit to be changed QUANT CALIBRATION Q Q 26 Press ZERO BASE to zero the instrument with the current method See page 4 To calibrate the system press return to the QUANT page and press CALIBRATE The QUANT CALIBRATION graph will be displayed and the instrument will prompt for each standard and replicate in turn As the measurements of the standards proceed the datapoints are marked on the graph When all the standards have been measured the system calculates the equation rescales the graph then draws and displays the line of best fit on the graph A calibration can be stopped by pressing the STOP function key The calibration will be aborted and the software will return to the QUANT STANDARDS page Any values obtained will be lost If a calibration has not been done pressing RUN causes the warning prompt CANNOT RUN WITHOUT CALIBRATION to appear otherwise it takes a sample measurement and switches to the Quant Results screen UV Issue 2a 12 2000 QUANT 0 0000 50 000 COEFFICIENT 0 9999 VIEW RESULTS Switches to the Quant Results page if there are results QUANT PAGE Switches back to the Quant p
51. ky Golay smooth is performed on the data and in each case data points will be lost from either end of the data SMOOTHING No of POINTS POINTS LOST AT USED EACH END NONE 0 0 LOW 9 4 MEDIUM 17 8 HIGH 33 16 ORIGINAL Q This removes any manipulation and displays the spectrum as originally collected and specified by the scan method It will also clear any Compare spectrum TRACK TABLE Page lt TRACK LIST a The list shows the y axis values of the spectrum for the MARK ABS A WAVELENGTH nm wavelengths marked during TRACK The measured values 1 ara ane will be ABS T INTENSITY or 1D 2D 3D 4D depending on the current mode 2 002 463 0 VIEW GRAPH Switches to the TRACK page PRINT LIST Prints the list on the selected printer LIMS EXPORT Sends the results via the RS232 port SCAN GRAPH Returns to the SCAN GRAPH page a Ifa spectrum is saved then the track markers are also saved and will be displayed when the spectrum is reloaded UV Issue 2a 12 2000 15 SCAN PEAK TABLE Page Sane PEAK TABLE ABS A WAVELENGTH nm Q The list shows the positions and values of the peaks as 1PEAK 0 472 447 0 calculated by the function selected in MANIPULATE PEI ASS 3888 PEAKS The measured values will be ABS T INTENSITY or 1D 2D 3D 4D depending on the current mode and are sorted by wavelength Each marker is identified as a peak valley or zero crossing LIMS EXPORT Sends the
52. l results have been collected save the data QUANT METHOD Page r QUANT i ID WAVELENGTH 550 0 nm BANDWIDTH 2 0 nm I INTEGRATION 1s STANDARDS 3 REPLICATES 1 UNITS CURVE FIT LINEAR LAMP CHANGE 325 nm H 1 OPERATOR 1 UVCALC 0 MEASURE STDS YES CELL PROG AUTO REF MODE _______ L OFF ID Enter a description using the TEXT ENTRY screen ad The ID identifies the method and will be saved with the method and any results produced by the method WAVELENGTH Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 190 0nm to 900 0nm Unicam UV310 320 330 340 190 0nm to 1100 0nm Unicam UV550 190 0nm to 850 0nm If the wavelength reguires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 Fixed at 1 5nm Unicam UV310 320 330 340 Select from 4 2 1 5 1 and 0 5nm Unicam UV550 Select from 4 2 1 5 1 0 5 and 0 2nm INTEGRATION Enter integration time in seconds This sets the integration time for which the result is measured The current data will be lost if the integration time is changed STANDARDS Brings up the Standards Entry Page Use the up and down arrow keys to move through the list of standards When the standard to be entered or edited is highlighted press ENTER to display t
53. ld be reset to zero 56 UV Issue 2a 12 2000 SETUP a The LAMP ENERGIES are measured as follows TUNGSTEN HALOGEN LAMP Measured at 546 nm DEUTERIUM LAMP Measured at 250 nm MERCURY LAMP Measured at zero order LAMPS TUNGSTEN ON HOURS 987 ENERGY 99 D2 STRIKING HOURS 234 ENERGY 96 Hg OFF HOURS ENERGY RESET HOURS Displays a menu to choose whether the tungsten deuterium or mercury lamp is to be reset When IIo selected resets hours and measures energy at the appropriate wavelength ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE RESETTING ITS HOURS ENERGY Displays a menu to choose whether the energy of the tungsten deuterium or mercury lamp is to be measured ALLOW THE LAMP AT LEAST 10 MINUTES TO WARM UP BEFORE MEASURING ITS ENERGY CLEAR BOTH SAMPLE AND REFERENCE BEAMS BEFORE MEASURING LAMP ENERGIES SWITCH Hg Switches the Mercury lamp ON or OFF depending on its current status THE MERCURY LAMP SHOULD BE TURNED OFF WHEN NOT IN USE SWITCH D2 Turns the Deuterium lamp ON or OFF depending on its current status UV Issue 2a 12 2000 57
54. page is used for entry of method operator and sample identities in all applications and for entry of concentration units in QUANT and FIXED methods The title displayed at the top of the page depends on which parameter is being edited On entry to this page the name field is filled with the current value OPERATOR Enter up to 11 characters for operator ID METHOD ID Enter up to 11 characters for method description UNITS Enter up to 11 characters for Conc units SAMPLE ID Enter up to 11 characters for sample ID a Use the Arrow keys to move the cursor to the required character and press ENTER Once all the required characters have been entered press ACCEPT Numbers are entered using the numeric keypad If you make a mistake olololole will act as a backspace or CLEAR will clear the whole entry TEXT ENTRY DOIO OO lt Acts as backspace and clears the last character in the line ACCEPT Enters the names and returns to the calling screen CANCEL Abandons the naming operation and returns the calling page The original entry is not changed UV Issue 2a 12 2000 5 GENERAL SAVE RENAME This page is used for saving or renaming files from any part of the LOCAL CONTROL software The page is used to name a file and or change the ID Q On coming to the page the Filename field is ready for editing Use the Arrow keys to move the cursor to the required character and press ENTER
55. parameters have been set save the method Select from range GRAPH LOW 0 01 to 6 00 Sets the upper graph limits on the SCAN GRAPH page GRAPH HIGH must be 0 01 greater than GRAPH LOW Select from range 0 3 to GRAPH HIGH 0 01 Sets the lower graph limits on the SCAN GRAPH page GRAPH LOW must be 0 01 less than GRAPH HIGH Select from 315 320 325 330 335 340 D2 W Hg Selects the wavelength at which the source is changed between the Tungsten and Deuterium lamps Selecting D2 W or Hg overrides any changeover and the selected lamp will be used regardless of the wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE OPERATOR UVCALC CELL PROG REF MODE UV Issue 2a 12 2000 Switches to the TEXT ENTRY screen The operator name is automatically saved with the method and any spectra produced by the method Changing the operator name will not cause the current spectrum to be lost If User Log on is in operation the operator name cannot be changed Appears when the UVCalc software has been installed Switches to the UVCALC screen Appears when the Cell Programmer is fitted Switches to the CELL PROG screen Appears when the Cell Programmer is fitted Toggles the status of the Cell Programmer reference mode 11 SCAN SCAN PARAMETERS page function keys SCAN VIEW RESULTS Switches to the Scan Peak Table screen if any of the peak functions have been performed or the
56. printed on a second sheet ALL SEQUENTIAL Prints each result in the run on a separate sheet of paper ONE RESULT Prints the result currently displayed UV Issue 2a 12 2000 31 RATE Q Pressing RUN starts a measurement using the current method Q Pressing ZERO BASE zeros the instrument using the wavelength specified in the current method See page 4 MANIPULATE TRACK SECTION RESCALE SMOOTHING ABS DISPLAY ORIGINAL ANOTHER CELL TRACK Sets the start and stop time for the rate calculations SECTION Sets sequential start and stop times to enable rates to be calculated on up to four sections of the rate curve RESCALE Changes y axis scale automatically or manually SMOOTHING Allows three levels of smoothing to be applied to the Rate Curve ABS DISPLAY Sets the display of absolute absorbance values or absorbances relative to the first measurement s of the run ORIGINAL Resets the graph to display the data as originally collected ANOTHER CELL Only present if the Cell Programmer has been used Enables the results of another cell from the same run to be displayed TRACK Q To move the vertical cursor across the screen use the Left and Right Arrow keys The cursor always moves to a data point regardless of the displayed scales Pressing ENTER places a marker at the current time Q To delete a marker place the cursor on the marker and press CLEAR Q The x axis values are used to recalculate the rate of change of Absorban
57. r Run Starts instrument measurement according to current method Home Returns to Home page Zero Base Performs a zero or baseline as appropriate to application REMOVE THE SAMPLE AND ENSURE THAT BOTH SAMPLE AND REFERENCE BEAMS ARE CLEAR OR CONTAIN THE BASELINE SAMPLES RELEVANT TO THE ANALYSIS BEFORE ZEROING THE INSTRUMENT OR PERFORMING A BASELINE SCAN Indicator LEDs Lower green ON upper red STANDBY 4 UV Issue 2a 12 2000 GENERAL Software The Local Control Software is organised in a tree structure with all functions accessed initially from the HOME Page Movement between the software pages is controlled by function keys or by highlighting with cursor keys and pressing ENTER Home will always go to the HOME page The SCAN FIXED QUANT RATE and MCA applications are entirely separate Only one application can be operational at any one time Loading another application will overwrite any current data Local and Computer Control Q From switch on the instrument will automatically be under the HOME control of the LOCAL CONTROL software To enable control from an external computer press REMOTE on the HOME page Control oloo will go to the computer providing the instrument is idle REMOTE a To return control to the Local Control software press the HOME key Control will revert to the Local Control software providing that the instrument is idle and the PC software has relinquished control TEXT ENTRY Page This
58. second derivative This records the second derivative of the Absorbance spectrum 3D Selects third derivative This records the third derivative of the Absorbance spectrum 4D Selects fourth derivative This records the fourth derivative of the Absorbance spectrum PEAKS a This option enables the spectrum to be automatically searched for peaks valleys or zero crossing points Move PEAKS the cursor to one of the options and press ENTER to VALLEYS perform a search When the search is complete the PKS amp VALLEYS Raat fe ZERO CROSS spectrum is displayed with the peak positions marked For RATIO a peak to be found there must be more than 15 data points CORR RATIO between that point and a previous peak PK HEIGHT For RATIO and CORR RATIO enter the wavelengths as prompted All results can be viewed by pressing OlOLO O view RESULTS PEAKS Marks the 10 highest peaks 14 UV Issue 2a 12 2000 SCAN VALLEYS Marks the 10 lowest valleys PKS amp VALLEYS Marks the 5 highest peaks and 5 lowest valleys ZERO CROSS Marks the first 10 zero crossings RATIO Calculates the ratio 44 1 CORR RATIO Calculates the ratio A A3 A2 A3 PK HEIGHT Calculates the peak maximum relative to a local baseline Ratio Corr Ratio and Pk Height are not available when UVCalc has been installed SMOOTHING a This option offers a pop up menu to choose one of three smoothing algorithms Choices are NONE LOW MEDIUM and HIGH A Savits
59. t options are available ALL OVERLAY ALL SEQUENTIAL and ONE RESULT ALL OVERLAY Prints the results of all cells in the run together in batches of 4 TRACK and SECTION markers are not included ALL SEQUENTIAL Prints each result in the run separately TRACK and SECTION markers are included ONE RESULT Prints the result currently displayed TRACK and SECTION markers are included 36 UV Issue 2a 12 2000 MCA 19 01 98 0 281A 11 35 MCA 550 0nm ID TEST 1 MEASURE STDS YES STANDARDS 0 UNITS WAVELENGTH S 1 BANDWIDTH 2 0nm INTEGRATION 1s DELAY TIME 00 00 LAMP CHANGE 325nm OPERATOR Name CELL PROG AUTO REF MODE OFF CALIB PRINT SAVE VIEW VIEW RATE METHOD METHOD STDS RESULTS Press CALIBRATE 09 03 98 0 281A 550 0nm Didymium ABS ABS A 0 206 A2 1 605 STOP UV Issue 2a 12 2000 MCA Enter identities and concentrations of standards Enter wavelengths to be used When all components have been calibrated insert first sample and press RUN 09 03 98 0 281A 11 35 CA 550 0nm STD ID conc g 1 1 HOLMIUM 1 0000 2 DIDYMIUM 1 0000 SO Gris LL O us 4 5 6 7 8 9 10 STD10 CANCEL ACCEPT pa 09 03 98 0 281A 11 35 WAVELENGTH S 550 0nm nm x nm 11 586 13 15 17 19 KAT 113 115 117 119 CANCEL ACCEPT SCAN
60. the calibration accordingly It can only be performed if the Mercury Lamp Accessory Part Number 9423 UV9 0023E is fitted and switched on It should only be attempted if for any reason the instrument no longer achieves its quoted wavelength accuracy specification a The calibration will take at least 15 minutes Once the calibration is complete the Default Baseline must be run Q This operation will only need to be done if the existing calibration is corrupt the grating has been replaced or the monochromator drive has been replaced a Install the Mercury Lamp Accessory as detailed in the Mercury Lamp Accessory Installation and Maintenance Manual a Ensure the Mercury Lamp is ON Q Ensure the instrument is fully warmed up and the beams are clear Q From WAVELENGTH CALIBRATION page select CALIBRATION USING Hg LAMP and press CALIBRATE Q Once calibration is complete switch both the Mercury Lamp and instrument OFF Q Switch the instrument ON and allow to warm up Q Run the Default Baseline Deuterium Lamp Calibration a This option will optimise the wavelength calibration of the instrument by measuring the Deuterium lamp emission line at 656 1nm and fitting the calibration accordingly It should only be attempted if for any reason the instrument no longer achieves its quoted wavelength accuracy specification Q The calibration will take at least 10 minutes Ensure Deuterium Lamp is ON Ensure instrument is fully warmed up and the
61. the results from any cell in the run to be selected and displayed Enter the number of cells you wish to display RATE RESULTS Page Q The Rate Results page displays the Initial and Final Absorbance Initial and Final Time the change in absorbance per minute calculated Activity Correlation Coefficient of the best fit line and finally the smoothing parameter used If the rate curve has been tracked the Initial and Final Absorbance with the Initial and Final Time will reflect those chosen by the two cursors Shown below is the Rate Results page after choosing SECTION and the three sets of data represent results calculated for each section A similar display will be seen with TRACK 34 UV Issue 2a 12 2000 RATE RATE RESULTS 1 2 3 0 992 0 662 0 662 0 512 00 10 00 40 00 40 01 00 0 660 0 450 1630 1111 0 995 0 952 Low LOW VIEW GRAPH Returns to the RATE GRAPH page RATE PAGE Returns to the RATE PARAMETERS page SAVE DATA Brings up the SAVE screen from where method and data can be saved to disk PRINT Provides a hardcopy of the results i e RATE GRAPH and RATE RESULTS LIMS EXPORT Sends the results via the RS232 port Parallel Rate Measurements using the Cell Programmer a The 8 Cell Programmer can be used in conjunction with the RATE software to measure between 2 and 8 cells in parallel For the Cell Programmer to be used it must be ON with MODE set to AUTO The RATE METH
62. tifications and concentrations of up to 20 standards may be entered UNITS Switches to the TEXT ENTRY screen a Enter the units for the concentration WAVELENGTH S i Switches to the WAVELENGTH ENTRY screen Available wavelength ranges depend on the instrument and are as follows Unicam UV510 520 530 540 190 0nm to 900 0nm Unicam UV310 320 330 340 190 0nm to 1100 0nm Unicam UV550 190 0nm to 850 0nm 38 UV Issue 2 09 99 MCA If the wavelength requires the Deuterium lamp then this will be switched on The current data will be lost if the wavelength is changed BANDWIDTH Selection depends on the type of spectrophotometer Unicam UV510 520 530 540 Fixed at 1 5nm Unicam UV310 320 330 340 Select from 4 2 1 5 1 and 0 5nm Unicam UV550 Select from 4 2 1 5 1 0 5 and 0 2nm INTEGRATION Enter integration time in seconds Q This sets the integration time for which the result is measured The minimum is 1s and the maximum is 9999s The current data will be lost if the integration time is changed DELAY TIME Set a time in the range 00 00 to 99 59 using to separate minutes and seconds The number of seconds must always be entered explicitly a This sets the time between pressing RUN and the start of the measurement The range is from O to 99 minutes and 59 seconds This field is only available if UVCalc has been installed LAMP CHANGE Select from 315 320 325 330 335 340 D2 W Hg Q Selects the wavelengt
63. ve the data FIXED METHOD Page i FIXED i MODE ABS i i i ID SELECT SINGLEN WAVELENGTH S 550 0 nm i BANDWIDTH 2 0 nm i INTEGRATION 1s i DELAY TIME 00 00 i LAMP CHANGE 325 nm i OPERATOR i UVCALC 0 i CELL PROG AUTO i i REF MODE i OF L N MODE Select from ABS T CONC ABS Selects Absorbance T Selects transmittance CONC Selects Concentration mode Only available when SINGLE is selected and not available if UVCalc is installed Q CONC allows the result to be automatically multiplied by a factor to give concentration measurements If selected two further parameters are added to the method FACTOR and UNITS ID Enter a description using the TEXT ENTRY screen ad The ID identifies the method and will be saved with the method and any results produced by the method SELECT Select from SINGLE MULTI SERIAL 1 SINGLE This option is used to measure each sample at a single wavelength which is the same for each sample MULTI This option allows each sample to be measured at up to 20 wavelengths which are the same for each sample SERIAL This option allows a single wavelength measurement to be made at a different wavelength on up to 9 samples WAVELENGTH S SINGLE Use the numeric key pad to enter the required wavelength into the pop up box Press ENTER when finished MULTI A Use the up and down arrow keys to move to the wavelength to be entered
64. wavelength set THE MERCURY LAMP MUST BE SWITCHED OFF WHENEVER IT IS NOT IN USE Any current data will be lost if the lamp changeover wavelength is changed OPERATOR Switches to the TEXT ENTRY screen The operator name is automatically saved with the method and any data produced by the method Changing the operator name will not cause any current data to be lost If User Log on is in operation the operator name cannot be changed UVCALC Switches to the UVCALC screen a Only available if UVCalc is installed See UVCalc Manual MEASURE STDS Toggles between YES and NO a When YES and Standard concentrations have been entered from the Quant Standards page pressing Calibrate causes the instrument to prompt the user to put the standard in the beam and press Run to measure for each Standard in turn Q When NO pressing Calibrate causes the system to prompt for an absorbance to be entered manually for each Standard effectively enabling a calibration originating elsewhere to be entered CELL PROG Switches to the CELL PROG screen if fitted REF MODE E Toggles the status of the Cell Programmer Reference Mode if fitted 24 UV Issue 2a 12 2000 QUANT QUANT Page function keys QUANT DIDIDIPIg VIEW CALIB Switches to the QUANT GRAPH page if a valid calibration exists VIEW RESULTS Switches to the Quant Results page if there are any sample results SAVE METHOD Brings up the SAVE page from where t

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