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UT-3100

1. 8 Left Left position Corresponds to the left position of the first strip of microwells Keys and H are used for adjustment by 0 1mm 9 Right Position on the right side Corresponds to the right side position of the first strip of microwells Keys and H are used for adjustment by 0 1mm Microplate Washer User s Manual Tips of operation by keyboard In the vertical direction key means an upward movement while key means a downward movement in the horizontal direction key means a left movement while key means a right movement Note The position parameters have to be set up according to the longer pin of wash head and the first strip of microwells of ELISA plates otherwise the plate washing function can t be completed normally Meanwhile the drawing in the right side means the plate type to be select and the current setting position Microplate Washer User s Manual 3 3 Switch bottle Microplate Washer can switch between three wash bottles conveniently keys Al IVi and H are used to select Switch bottle and pressing START will enter the program of switching wash bottles as shown in Fig 3 9 Keys A and V are used to select bottle number or switching time The keys and H are used to modification the switching time When th
2. If 8 way wash head is used the edges with 8 microwells of the ELISA plate shall be put parallel to the wash head Similarly when 12 way wash head is used the edges with 12 microwells of the ELISA plate shall be put parallel to the wash head When installing the ELISA plates with your right hand catch the two sides of the long edge of the ELISA plate and from top to bottom put it into the cross groove and make the rear edge of the ELISA plate closely contact the rear edge of the nearest groove Note Please operate the instrument strictly according to aforementioned requirements otherwise it may lead to the fact that the dispensing volume is not correct the liquid spills over or the wash head is damaged 4 2 Washing the plates 4 2 1 Parameter confirmation Use A V H and H to select Plate washing press START to enter the parameter confirmation program as shown in Fig 4 1 From top to bottom and left to right the parameters are as follows 21 Microplate Washer User s Manual 1 Program No Code number of the current program use and SELECT to switch between changing program number by 1 or 10 to select program Press 2 Settings If the parameters of the current program need modification the parameters can be edited in the Settings menu 3 1 12 F Means that the plate type setting with code number of 1 is selected 12 mean
3. Use the h and M keys to select Time Speed and Extent of plate shaking And Press and to adjust values Shaking intensity and time are adjustable There are 10 levels of Speed the bigger value the faster Extent is the distance of moving back and forth can be set 0 1 2 0mm Maximum shaking time is 23H 59M 59S Intensity of plate shaking in Plate washing menu depends on intensity set on Plate shaking menu In plate shaking menu incubation settings is also available For more details about incubation you can reference to chapter 4 5 Maximum incubation time is 18h Plate shaking and incubation can be carried out independently Press START to save changes and shake plate Shaking time is displayed Double press SELECT key to start counting of incubation time Press CANCEL to quit Microplate Washer User s Manual 3 1 4 Stroke settings User can adjust the stroke scope of motor on horizontal and vertical directions Keys A and MV are used to select Stroke Settings and press START to enter the stroke settings program as shown in Fig 3 6 Stroke settings Horizontal direction 136 1 mm Vertical direction 19 1 mm Fig 3 6 Stroke settings Keys A and V are used to select Horizontal direction or Vertical direction Keys and H are used to adjust the maximum stroke scope Press START to save the chang
4. 0 START cont CANCEL Fig 4 9 Rinsing interface 28 Microplate Washer User s Manual Time The rinsing time can be set from 1 60 seconds After setting the rinsing time press START to save and start rinsing the pipeline Status will show the real rinsing time Note Rinsing and priming time of pipeline should not be too long usually it shall be about 8 seconds 29 Microplate Washer User s Manual 4 5 Incubator 4 5 1 Parameter settings Use A M and H keys to select Incubator Press START to enter the incubator settings interface as shown in Fig 4 10 Select the item using and M and press and H to edit the parameters 00 06 00 00 08 00 Warning 5 mye Warning 5 1800 Time 00H 30M 00S Time 00H 30M 00S Temperature Ambient Temperature 370 Click START to off incubator Click START to incubate a Turn off incubator b Turn on incubator Fig 4 10 Incubator parameter settings Warning when incubation is complete the system will emit a warning tone It can be set to Off or 1 10 with Off meaning no warning and 1 10 indicating the number of warning signals Time incubation time can be set at 0 18 hours Press the SELECT key to switch between 1 second and 10 second increments Temperature the incubation temperature can be set to Ambient 25 30 or 37
5. Press START to save the settings and start the incubation program Press CANCEL to quit without saving Note After setting the temperature at 250 30 or 37G the incubator works continually While setting the incubator temperature to Ambient the incubator turns off automatically 4 5 2 Incubation process The incubation mode is displayed in the top right corner when the incubator is set to 25 30 or 37 as shown in Fig 4 10 b The first row displ ays the incubation processing time while the second row indicates the programmed incubation time The incubation mode will not be displayed when the temperature is set to ambient Place a microplate on the incubator and press SELECT twice to display the incubation mode top right corner When the programmed incubation time has elapsed the instrument will emit a tone according to the programmed settings 30 Microplate Washer User s Manual Warning When the incubator is heated don t touch the incubation plate with hand in order to avoid or prevent scald furthermore the incubator is in a state of high voltage power supply so when the instrument is working it is forbidden to touch the incubation plate with hand or conducting objects as ironware etc in order to avoid electric shock 31 Microplate Washer User s Manual 5 Maintenance 5 1 Regular maintenance of the worktable The worktable usually has remnant liquid
6. and 59 seconds 13 Shake time Can be set from 0 seconds to 23 hours 59 minutes and 59 seconds If the washing time is more than twice including twice the system will ask for a parameter setting for the last washing as shown in Fig 4 2 c The upper right corner of the screen displays Last time and the detailed parameter setting is the same as items 8 13 Note When some specific parameters of relatively big scope are set up key SELECT can be used to switch the increase decrease coefficient to 0 1 1 or 10 in a flexible way And show the current increase decrease coefficient value in the right side of the revised parameters 4 2 3 Plate washing Note During the plate washing open the incubator set temperature at 250 30 or 37 the incubating status will display on the top right corner of the screen three times press SELECT key the incubator works and counts time then give out alert when time is up When the parameters in Fig 4 1 are confirmed to be correct and the number of the program is selected the number of the program is highlighted press START to get into the plate washing procedure as shown in Fig 4 3 The settings of strip number and position are as follows Program No 1 Strip No 8 Position Miki Press START to continue Fig 4 3 Main interface of plate washing Strip No means the strip number of plates to be washed When 12 pin wash
7. and V type respectively 3 Settings Enter the plate type program settings and the detailed operation is similar to that of 4 2 Plate setting 4 Wash times Set up the number of times of plate washing which can be set from 1 99 times 5 Wash Mode The plate washing method can be selected between plate washing and strip washing 6 Wash strips According to the type of the wash head the number of strips is initialized into 8 or 12 strips Note The wash head can only be set up in Plate Settings please see 4 2 Plate setting for details the Wash strips is similar to 4 2 3 Plate washing operation and is able to be revised directly 7 Volume Dispensing volume the dispensing volume of wash liquid for each well can be set as 10 3000uL SELECT is used to switch between changing the volume by 1 or 10 8 ASPR mode Aspirate method single aspirate or double aspirate 9 Bot wash Bottom washing whether the bottom washing is carried out or not 23 Microplate Washer User s Manual 10 ASPR time Aspirate time set up the aspirate time adjustable from 0 1 to 10 seconds SELECT is used to switch between changing the time by 0 1 or 1 11 Shk grade Plate shaking grade can be set up from 1 3 grades of plate shaking intensity 12 Soak time The time between dispensing of wash liquid completed and starting to aspirate the wash liquid can be set from 0 seconds to 23 hours 59 minutes
8. parameters are as follows Mode Indicates the liquid aspirate method which can be selected between single aspirate and double aspirate Time The liquid aspirate time can be set from 0 1 to 10 0 seconds SELECT is used to switch changing the time by 0 1 and 1 Plate Choose the plate type program 1 indicates the program number and 12 F means that this program is used for 12 pin wash head and the position is set up according to the flat type ELISA plates Strips Set up the strip number for single aspirate Position Liquid aspirate strip settings Pressing the key will confirm the liquid aspirate while pressing the key will cancel the liquid aspirate After setting the parameters press START to save and start single aspiration 4 3 2 Single dispense As shown in Fig 4 6 b the single dispense means that only add liquid to the microwells according to fixed parameters no aspiration The parameters are as follows Bottle bottle number for dispensing About switching bottle please refer to 3 3 Switch bottle Volume The dispensing volume of wash fluid for single well can be set from 10 3000uL SELECT is used to switch between changing the volume by 1 or 10 Plate Choose the plate type program 1 indicates the number of the program while 12 F 26 Microplate Washer User s Manual means that this program is used for 12 pin wash head and t
9. the waste liquid is pumped to waste bottle by vacuum pump Meanwhile three wash channels of the instrument are automatically switched over by a solenoid valve and are controlled in real time by a relevant liquid surface sensor which can feed back the liquid surface status of the current working bottle The wash bottle liquid aspirate pump and dispensing pin thin metal pipe of the wash head make up the dispensing routes of Microplate Washer dispensing of the three kinds of the wash liquid and the distilled water is controlled by four corresponding solenoid valves respectively The liquid aspirate pin relatively thick metal pipe of the wash head the waste bottle and the vacuum pump make up the draining routes of Microplate Washer The negative pressure generated by the vacuum pump reaches the liquid aspirate pin via the waste bottle while the liquid within the microwells gets into the waste bottle under the atmospheric pressure Microplate Washer User s Manual 2 Installation of the instrument First of all for Microplate Washer prepare a place which meets the following requirements e Environment temperature 10 C 30 C e Relative humidity lt 70 e No direct sunlight or high dust concentration e No electro magnetic radiation e With a sufficiently big smooth and firm tabletop 2 1 Unpack Unpack the package and transportation materials of the instrument Keep the packing box and packing material for future storage an
10. wash bottle using Switch bottle or refill the current bottle with wash liquid 3 Waste bottle full Check the waste bottle If it is full empty the bottle and reconnect it If the bottle is not full check to make sure that the liquid level sensor cable is connected correctly to the port on the back of the instrument Note 1 When using the instrument if the user meet an error which can not be solved by yourself or some error appears repeatedly please get in touch with the seller 2 For the instrument of different editions its configuration may be subject to a change But the change does not influence the performance and application of the instrument so please use it at ease 34 Microplate Washer User s Manual Declaration We reserve the sole right to the interpretation of this manual Pictures in this manual are only for example may be different from the actual displays on the product They shall not be used for other purposes Unless authorized by us with written consent no person or organization has the right to copy edit or translation the content of this manual Instructions on optional parts of Microplate Washer e This manual is written for the operation of Microplate Washer with incubator and 3 wash bottles e Product with different version may be different with optional parts please operate according to following instructions Microplate Washer without incubator users don t need to read 4 5 Incu
11. which will corrode the metallic elastic claw And if there are too much liquid it might overflow the edge of the worktable and leak into the machine It is essential to eliminate the remnant liquid frequently on the worktable A pair of tweezers is used to clip a small piece of absorbent cotton and to clean the remnant liquid But be careful that the heads of the tweezers can not scratch the surface of the worktable Note Any organic solvent oil and corrosive liquid can not be used to clean the worktable or the surface of the instrument 5 2 Regular maintenance of the wash head The wash head is one of core parts of Microplate Washer Its status will affect the accuracy of the dispensing volume and the quantity of washing so it is very important to maintain the wash head The suspended particulate substance in rinsing liquid will block up the dispensing pipe so please use fresh rinsing liquid and frequently check whether there is suspended substance existing or not The dispensing and aspirate pins may be blocked up as a result of the crystallization of the rinsing liquid so in order to prevent the crystallization from appearing be sure to remember to carry out the rinsing RINSE operation all the time after the work is completed If the dispensing volume of one row is found to be lower than other rows obviously it shows that the corresponding dispensing pin thin and short pin is blocked up when the remnant liquid volume of a row is fou
12. EP gt amp a a a a EEX Hel This symbol means that the labeled item is hot while the instrument is in use Don t touch the labeled item as you could be burnt The symbol is labeled on the lamp support of optic system This means that the labeled item could lead to personal injury and or damage to the analyzer The symbol is labeled beside the power outlet and some external interface The symbols for SERIAL NUMBER The serial number shall be after or below the symbol adjacent to it The symbol means the product is in vitro diagnostic medical device The symbol indicates the manufacturer and its address after which are shown its name and address The symbol indicates the date of manufacture after which are shown its manufacture date The symbol indicates EU representatives of the manufacturer and their addresses after which are shown their names and addresses The symbol indicates biological pollution marked in the part where the instrument contacts the clinical reagent The symbol appears in black side and yellow background The symbol means that the environment of instruments must be dampproof in the course of transport and instrument must be kept in a dry environment This means that instrument should handle with care in the course of transportation so as not to damage it The symbol means the instrument packaged should not be upended at any time The symbol means that the level piled up can t
13. ER WASTE WASH1 WASH WATER WAS TE WASH WASH 2 p WATER lt Tubing connectors Waste liquid wash liquid and distilled water bottles Fig 2 1 Wiring diagram of tubing and alarm signals Note The instrument works with the principle of atmospheric pressure so the bottleneck of the waste bottle as well as all tubing interfaces must be sealed up 2 3 Install the wash head e Respectively rotate two ends of the dispensing tube and the draining tube to the corresponding colored tube joints e Hang the wash head on the left side of the supporting bracket and make sure that the two pins at side fall into the groove of the supporting bracket 2 4 Start the instrument e Fill the wash bottles and the distilled water bottle with cleaning wash liquid and distilled water respectively Take out the power line in the accessories bag and have one end connected to the power inlet of the rear plate while the other end connected to the 220V power supply e Start the instrument by pressing the power switch at the back of the instrument Microplate Washer User s Manual 3 Settings and adjustment of the instrument In the software of Microplate Washer a system setting menu has been provided for users to set up the system To make sure that the ins
14. Laboratory Equipment oo A www mrclab com CE Net THE STANDARDS STITUTION OF SRAL Operation Manual for Microplate Washer UT 3100 PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3 Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC VER 01 9 12 Microplate Washer User s Manual How to use this manual It s greatly appreciated you become the user of Microplate Washer Be sure to read this manual carefully before operating the instrument In order to obtain the best result it is important for you to get familiar with our instruments and their functions before performing clinical diagnostic tests This User s Manual is the operation instructions for Microplate Washer which including the contents of instrument installation daily use and maintenance etc Please reserve all the packing materials for the use of deposit transportation and maintenance in the future You may contact with your distributor if there is any problem As to the instruments of different version or configuration their functions are slightly different Notes Tips advices and suggestions will be presented in italics for distinction Warning The warning shall be strictly abided by in order to ensure that the instrument may works normally and the test results may be correct and authentic Black letters are adopted for distinction Microplate Washer User s Manual Symbols on the instrument L
15. XXX Plate washing Plate settings ASPR DISP Switch bottle PRIME RINSE Adv settings Incubator Switch off Fig 3 1 Main interface of the system Microplate Washer User s Manual 3 1 Advanced settings Set the systematic parameters such as LCD contrast ratio work temperature of the incubator limit stroke of motor and system s hang up time etc On the main interface the keys H and H can be used to select Adv settings and pressing key START will enter the advanced settings program as shown in Fig 3 2 The keys and O are used to select the corresponding programs Contrast ratio settings Hang up settings Plate shaking Stroke settings Asp pump speed settings Fig 3 2 Advanced settings interface 3 1 1 Adjust the LCD contrast ratio The keys A and V can be used to select Contrast ratio settings And pressing key START will enter the contrast ratio setting program as shown in Fig 3 3 Fig 3 3 Interface of contrast ratio settings If the key H is pressed the contrast ratio increases and the color becomes darker if the key H is pressed the contrast ratio decreases and the color becomes lighter After adjusting to optimum value press START to finish the adjustment and get back to previous menu The new value of contrast ratio is saved in t
16. adland 14 eA e A ENO RETORNA e EEEE ainiai aaa ias aidai adis a a aa aaa a adidas 14 S Pe eu ed Fo Shaking 12 iai ae ata aaa ai tas aa A e ii ae aa ai 15 3 134 Stroke Settings a ia iii iii ii i i i i i i ii a a andes 16 3 1 5 Aspirate pump speed settings LL aaa a aaa kaaae aka aaa aaa aa aaa aaa aaa aa aaa aaa aaa 16 3 2 Plate SOMOS stai A ae ai a r ai a ia bai 17 39 9 SWiteh DOLE isian i rd ri i i ra Li L a i k Lk O 19 3 4 Turn off the SYSIC MS L ninkai a sa ie as a bai a is 20 4 Operation of the instrument ccccccceeeeeeeeeceesseeeeeeeenasseeeeeseanaeeess 21 4 1 Worktable and ELISA DIAT6S i kuni iii i a i i ieieldts 21 4 2 Washing the DIAIES 1 A ara a a epee a a a A A 21 4 2 1 Parameter Confirmation ccccccecsceeeceneeeeeeeeeeeeeeeeaeeeeeeeeaeeeeseeaeeseeseaeeeeseeeeeesenaeeeeesaas 21 4 22 Parameter SeCuINGS keee ledais iela iais iai saaa a deni ae hia chained 22 4 2 34 Plate WASTING esi sese EEE seen veatteasetes ais Drei Ais i di i S 24 4 3 Single aspirate single GISPENSE cccceceeceeeeeeeeeneeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneeeaaees 26 ASA Single aspirate iada iai i ss i i i i i i i i iais 26 4 3 2 Single dispense 2 d iai aaa aaa a a i a r a a a a i a a a as 26 AAs FIG BNS 5 ite ii a e as i a k i a a a ae 28 Microplate Washer User s Manual AAT E a EIA EAER EER i eeu E E leet le i a a a a a ee oe 28 4 42 RINSE Assis asa ili a a a Ava i a a a i i iti ss 28 4 5 ME eA eh htc et i St a ck Ses al i
17. bator system displays No incubator please exit Microplate Washer with only one wash bottle users don t need to read 3 3 Switching bottle system displays No switching for single channel washer please exit Different optional parts will not affect the performance of product If you have any question please contact our service center or your supplier 35
18. c when the instrument is in motion in order to avoid electric shock Please wear the rubber gloves while carrying out maintenance please wash your hands to avoid virus infection after each operation finishes e Don t let hands or other positions of body touch the reagent or the patient s sample if the wounded part touches the patient s sample carelessly please wash it timely and see a doctor for examination e Blocked wash head will cause bad aspiration and dispensing In this case please rinse the wash head with distilled water and then dredge the blocked hole with a thin pin If the problem can not be solved this way you need to replace the wash head e The product is a bench type testing device without issues on taboo e The ground wire of the power line of this instrument has ground connection to avoid electric shock The ground wire has to be connected to ground e The AC electric supply must be stable and is forbidden to share the power with high power electric apparatus While pulling out the power line be sure to catch the plug but not the power line e Turn off the power supply immediately and contact the seller if the instrument gives out smog peculiar smell or strange sound e Be sure to cut off the power supply and switch off the main processor when carrying out maintenance or dismantling the outer cover due to other reasons e Pharmaceutical affairs law and regulation it is forbidden to refit the medical in
19. conds Program No 1 Settings 1 12 F Plate 12 Pin 350uL 1 3Times BW N BW Y Single Double Asp 2 0 3 0 s Soak 10 15s Shk10 15s START to cont CANCEL Fig 4 1 Confirmation of plate washing parameters 4 2 2 Parameter settings When the parameters in Fig 4 1 do not meet your requirement A and V are used to select 22 Microplate Washer User s Manual Settings and pressing START will get into the parameter setting program as shown in Fig 4 2 Program No 1 Program No 1 Plate 1 12 F Settings ASPR mode Single Bot wash N Wash times 3 ASPR time 2 0 S Wash mode plate Shk grade 1 Wash strip 8 Soak time 00H 00M 10S Volume 350uL Shake time 00H 00M 10S a Item parameter one b Item parameter two Program No 1 Last time ASPR mode Double Bot wash Y ASPR time 2 0 S Shk grade 1 Soak time 00H 00M 15S Shake time 00H 00M 15S c Item parameter three Fig 4 2 Item parameters The parameter description and the detailed settings are as follows 1 Program No Code number of the program keys H and H can be used to select the program SELECT is used to switch between changing program number by 1 or 10 2 Plate Plate type settings the plate type settings program is respectively 1 10 among them the letters in the bracket AA B AA means a wash head while B means a plate type Flat type U type
20. d transportation over again Take out the packing list and check the accessories Take out the instrument Note Keep the packing box and packing material for future storage and any later transportation Please contact the distributor if any accessory is missing 2 2 Install the bottles and tubing Open the packing box of bottles and take out the bottles tubing alarm signal line etc Install the bottle Towards the rear side of the instrument from left to right sequentially put the waste bottle wash bottle 1 wash bottle 2 wash bottle 3 and distilled water bottle as shown in Fig 1 1 and Fig 2 1 Connect the tubing As shown in Fig 1 2 and 2 1 towards the rear side of the instrument from left to right connect the tubing of the waste bottle wash bottle 1 wash bottle 2 wash bottle 3 and distilled water bottle according to the sequence of the bottles Among them the negative pressure port of the waste bottle is connected on the top of the tubing corresponding to the same interface color Connect the signal line As shown in Fig 2 1 in the face of the rear side of the instrument from left to right connect the signal lines of the waste bottle wash bottle 1 wash bottle 2 wash bottle 3 and distilled water bottle according to the sequence of the bottles Microplate Washer User s Manual DON f Liquid level alarm ee connectors WASTE WASTE WASH1 WASH 2 WASH WAT
21. e bottle number change in the bottom of the interface shows switched to bottle X as shown in Fig 3 9 b Press START to start switching the wash bottle otherwise current bottle number remains as shown in Fig 3 9 a wash bottle will not be switched During switching procedure first of all rinsed the pipelines with distilled water About the rinse time settings please refer to section 4 4 2 Rinse The status shows Rinse X and then prime the pipelines with the wash liquid from the target wash bottle The status shows Prime X During operation pressing key CANCEL will cancel the switching It will keep using the wash liquid from current wash bottle Select wash bottle 2 3 Time 8 S Select wash bottle 2 3 Time 8S Status 0 Status 0 Bottle 1 no switching Switched to bottle 2 a No switching prompts b Switching prompts Fig 3 9 Switching the wash bottles Microplate Washer User s Manual 3 4 Turn off the system If it is confirmed that the plate washing operation has been completed user can directly switch off the instrument or the user can select Switch off program on the main menu then the system automatically rinses the pipelines with distilled water saves the current program code number of the plate washing program and ask user to switch off the system as shown in Fig 3 10 Press START to switch off press key CANCLE to return to the main men
22. ent Microplate Washer User s Manual 1 Power switch 8 Interface 1 of waste bottle 2 Fuse 9 Interface of wash bottle 1 3 Power inlet 10 Interface of wash bottle 2 4 RS 232 interface 11 Interface of wash bottle 3 5 Sensor interface 12 Interface of distilled water bottle 6 Label 13 Air vent 7 Wiring diagram of tubing 14 Interface 2 of waste bottle 1 4 Keyboard ASPR 600622 2e0oesc Fig 1 3 Schematic diagram of keyboard Start a process on Save the parameter modifications Cancel the current operation CANCEL Discontinue the underway treatment Cancel saving and quit the parameter modification A Upwards v Downward T Change the parameters increase upwards left Change the parameters reduce downwards right PRIME Start the priming procedure shortcut key SELECT Switching between rough adjustment fine tuning RINSE Start the rinse procedure shortcut key ASPR Start the single aspirate single dispense procedure shortcut key Microplate Washer User s Manual 1 5 Principle of the instrument Microplate Washer is composed of a wash bottle a liquid aspirate pump a wash head a vacuum pump and a waste bottle Certain volume of the liquid in the wash bottle is pumped by the liquid aspirate pump and then evenly dispensed into the microplate by the wash head after special treatment such as soak and shaking etc
23. es at the same time the corresponding motor moves to the preprogrammed position automatically Press CANCEL to quit without saving 3 1 5 Aspirate pump speed settings Keys _ and are used to select Asp pump speed settings and press START to enter the pump settings program as shown in Fig 3 7 Through keys and to set suitable pump speed value Key is used to open the pump Key is used to close the pump and the status bar will show on or off respectively Asp pump speed settings Pump speed 5 Status Off Fig 3 7 Asp pump speed settings Note Before leaving factory the instrument has been adjusted to optimum position so please don t adjust it at will Otherwise the plate washing effect of the instrument will be influenced Microplate Washer User s Manual 3 2 Plate settings User can preprogram 10 kinds of plate parameters Keys A V and H are used to select Plate Settings and pressing START will get into the plate settings program as shown in Fig 3 8 A and V are used to select the parameters which need modification and keys and are used to modify relevant parameter values Press START to save and return and press key CCANCLE to quit without saving Plate No 1 posi
24. exceed 8 layers as not to damage instrument The symbol indicates temperature range of the analyzers during storage and transportation Microplate Washer User s Manual Warning and Safety Indications This instrument is only used for in vitro diagnosis please carefully read the following warnings before the usage and strictly obeys them e The articles and materials used by some test items have biological danger Be sure to wear the protective clothing and the glasses when operating the instrument and put down the protection cover e Some steel pins of the wash head are sharp so touching them may lead to bodily injury Don t put your hand or finger in the area of the worktable when the instrument is in motion Be sure to turn off the power before replacing the wash head It is forbidden to touch the wash head when the instrument is in motion When the instrument is in motion be sure to turn off the power switch at once if the waste bottle is turned over At this moment the instrument probably discharges a small amount of waste liquid through a device in the bottom and this waste liquid must be dealt with according to the biological dangerous cargo When the incubator is being heated don t touch the incubation plate by hand in order to avoid or prevent scald furthermore the incubator adopts high voltage power supply so it is forbidden to contact the incubation plate with hand or such conducting objects as ironware et
25. he FlashROM of Microplate Washer When the instrument started next time the LCD contrast ratio is set up according to the new value During adjustment press CANCEL to quit without saving Microplate Washer has 64 contrast ratio grades 3 1 2 Hang up settings User can set up the hang up time Keys H and H are used to increase or decrease time Pressing 14 Microplate Washer User s Manual SELECT can switch between the operation of changing the time by 1 or 10 minutes Press START to save and quit and press the key CANCLE to quit without saving as shown in Fig 3 4 Hang up settings 20 min Fig 3 4 Hang up settings If there is no operation within the preprogrammed hang up time instrument will rinse the pipeline automatically and get into a hang up status In the hang up status press any key to quit and get back to operation when returned from hang up status the instrument will automatically prime pipelines with preprogrammed wash liquid 3 1 3 Plate shaking The user can shake plate during washing procedure or independently The and M keys are used to select Plate shaking Press START to enter the Plate shaking interface as shown in Fig 3 5 Plate shaking Time 00H 00M 60S Speed 6 Extent 1 7mm Incubator settings Click START to shake plate Fig 3 5 Plate shaking
26. he position is set according to the flat type ELISA plates Strips Set up the strip number for the single dispense to cancel dispensing Position strips for dispensing Press to confirm dispensing and press After setting the parameters presses START to save and start single dispensing by the process of dispensing key CANCEL will be returned to stop operating 27 Microplate Washer User s Manual 4 4 Prime Rinse Select PRIME RINSE menu use M H and and press START to access as Fig 4 7 Fig 4 7 PRIME RINSE menu 4 4 1 Prime Press and M to select Prime and START to enter Prime menu Bottle 1 Priming Time 8S Status 0 START cont CANCEL exit Fig 4 8 Prime interface After setting the priming time press START to save and start priming Status shows the elapsed priming time Bottle 12 indicates that the liquid in wash bottle 1 is used for priming Press CANCEL to stop and exit Advice Set the priming time for 6 8 seconds to enhance accuracy of liquid distribution 4 4 2 Rinse Select RINSE using A Mi H and H and press START to get into the pipeline rinsing program as shown in Fig 4 9 Press keys H and H to revise the rinsing time Rinsing Time 8S Status
27. head is selected the strip number can be selected between 1 8 strips When 8 pin wash head is selected the strip number can be selected between 1 12 strips This parameter is the same as the Wash strip of 4 2 2 Parameter setting But it is only for the washing of this time will not be saved 24 Microplate Washer User s Manual Position shows the position to be washed and the keys A and V are used to select the modification setting Pressing H will confirm the washing while pressing H will cancel the washing After confirming the strip numbers and position put the ELISA plates to be washed on the worktable and press START to start washing as shown in Fig 4 4 Program No 1 Strip No 8 Position Bann Dispensing Fig 4 4 Plate washing status The Program No shows the selected program At the right side 1 3 means wash three times and it is the running the first time at present The middle bottom on the screen will shows status information such as Draining Bottom washing Dispensing Shaking and soaking etc at the same time the right side shows the percentage or time of the current operation During the procedure of plate washing pressing CANCEL will cancel the plate washing and show error information as shown in Fig 4 5 After a short while the system will automatically return to the parameter confirmation scree
28. n Program No 1 Strip No 8 Position Sas User Abort Fig 4 5 User stopping screen Note During washing procedure if the washer detects that the wash bottle is empty or the waste bottle is full it will stop the operation automatically and display the bottle number at the left bottom of screen At this time fill corresponding empty wash bottle with wash liquid or pour the waste liquid from waste bottle Make sure that you have installed the bottle well Press CANCEL to exit or press START to continue 25 Microplate Washer User s Manual 4 3 Single aspirate single dispense Select the ASPR DISP by keys Al V H and H and pressing START will get into the single aspirate single dispense program as shown in Fig 4 6 Press and in the options of Function to switch between Single aspirate and Single dispense Function Single aspirate Function Single dispense Mode single Bottle 1 Time 1 2 S Volume 350uL Plate 1 12 F Strips 8 Plate 1 12 F Strips 8 Position Mai ai Position See START cont CANCEL exit START cont CANCEL exit a Single aspirate b Single dispense Fig 4 6 Single aspirate single dispense interface 4 3 1 Single aspirate As shown in Fig 4 6 a single aspirate means that only aspirate the liquid according to fixed parameters no dispensing operation The
29. nd to be higher than other rows obviously it shows that the corresponding liquid aspirate pin thick and long pin is blocked up When the instrument is in a stand by status take off the wash head from the supporting bracket Use a thin pin to dredge the blocked dispensing or aspirate pins Then put the wash head back to the supporting arm and press RINSE to rinse it If the dispensing pin is blocked up seriously the above mentioned course need to be repeated many times 5 3 Replacement of the fuse The specification of the fuse is T3 51AL 250V and the steps of replacing the fuse are as follows e Take out the fuse from the accessory bag Unscrew two fuse caps on the rear plate of the instrument Have the fuse clipped in the middle of the metal reed on the cover and then fasten the fuse caps onto the fuse wire base 5 4 Treatment of the waste bottle The sensor within the waste bottle can detect the height of the liquid level When the waste liquid 32 Microplate Washer User s Manual reaches a certain height the software of Microplate Washer will refuse any liquid aspirate operation At this moment please deal with the waste liquid in time Hold the bottle lid of the waste bottle and lift it slightly Hold the bottle body of the waste bottle and rotate it clockwise until the bottle lid is broken away from the bottle body Dump the waste liquid and put the bottle body back to the original place Hold the b
30. nl ew Ms Die Hc pedal tno 30 45 1 Parameter SOUmingsize ati as das ads ni aa as S ai aaa da 30 4 5 2 IMCUDALION PlOCOSS Ak un nana a aaa 30 54Mainten ANC Os aa ua dai a a aa a a a a a a a o Ads 32 5 1 Regular maintenance of the worktable cccccceceeeeeeeeeeeeeeeeeeeeeeeeteeeeeeeeeeeeeee 32 5 2 Regular maintenance of the wash Nead cccceeesseeeecceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee 32 5 3 Replacement of the fuse ccccecceeeeeeeeeeeeeeeceeeeeeeeeeeeeeeeaeeeeeeeeeeeseecaeeeeeeeeeeeeenes 32 5 4 Treatment of the waste bottle ceeeee cee ecceeeceee eee eeeeeeeeeeeeeeeeeeseseeaaeeeeeeeeeeeeeeea 32 6 System error and solutions sericsex ccna ates cane recevieene eevee 34 PO CLAN AOM east ao i ia i I a a a S i amma a 35 Microplate Washer User s Manual 1 Brief introduction of the instrument 1 1 Brief introduction of product Name of the product Microplate Washer Features of the product English operating system large screen liquid crystal display and graphic interface Support for single and three channel wash system apply to washes flat U and V bottom ELISA plates and strips as well as standard 96 well or other types of plates Convenient programming wash time liquid volume and soak time programmable Plate washing row washing and row skip washing available Automatic positioning Manual positioning to ensure the precision The design of double pin wash head reduces cross contamination Wa
31. ottle lid of the waste bottle and cover it on the bottleneck Hold the bottle body of the waste bottle and rotate it counterclockwise until it is screwed tightly Note 1 If the bottle lid is fastened too loosely the air tightness of the waste bottle can t be ensured and the liquid aspirate effect is influenced but if the bottle lid is fastened too tightly it will damage the bottle lid 2 When opening the waste bottle don t rotate the bottle lid and you can only rotate the bottle body of the waste bottle so as to prevent the cable of the sensor from being cut off 3 Microplate Washer is a precision instrument so please pay attention to maintaining it every day 4 The wash head of the instrument is a precision component To add samples to the ELISA plate please don t add whole blood or other unexpected things so that the wash head is not blocked up 33 Microplate Washer User s Manual 6 System error and solutions When there is a system error Microplate Washer will stop all movement of mechanical parts and report it to the user The buzzer gives out a long sound and the error information shows on the liquid crystal display Wash bottle empty distilled water bottle empty and waste bottle full are the most common error information Detailed treating methods are as follows 1 Distilled water bottle empty refill the distilled water bottle with distilled water 2 Wash bottle empty either switch to another
32. s a 12 way wash head while F means that the position of this program is set up according to F type 4 Plate Plate washing methods 5 12 Pin Shows that it is using the setting of 12 way wash head 6 350uL Means that the dispensed liquid volume for each hole is 350uL 7 12 Means that the bottle 1 will be used for washing About the switching of bottles please refer to 3 3 Switch bottle 8 3 Times Wash 3 times the number of times of plate washing 9 BW N BW Y No Bottom Washing Bottom Washing if the wash time is more than twice including twice the previous plate washing cycles have no bottom rinsing but the last wash cycle has bottom rinsing 10 Single Double Single aspirate Double aspirate if the wash time is more than twice including twice the previous wash cycles are single aspirate but the last one is double aspirate 11 Asp2 0 3 0s Aspirate 2 0 seconds 3 0 seconds if the wash time is more than twice including twice the liquid aspirate time of the previous wash cycles are 2 seconds but in the last cycle the aspirate time is 3 seconds 12 Soak10 15s If the wash time is more than twice including twice the soak time of the previous wash cycles are 10 seconds but in the last cycle the soak time is 15 seconds 13 Shake10 15s If the wash time is more than twice including twice the plate shaking time of the previous wash cycles is 10 seconds but in the last cycle the shaking time is 15 se
33. sh and waste bottles have autoalarm function with liquid surface detecting 8 and 12 way wash head available Incubation function optional Microplate Washer User s Manual 1 2 Specifications of the instrument Wash head 8 way or 12 way No of wash profiles 10 Capacity of the storage Wash bottle distilled water bottle and waste bottle 2L each bottle bottle respectively Washing protocols 100 Washing cycles Max 99 Soak shaking time 0 24 h Mode Plate amp strip wash Accuracy 5 Residual volume lt 1l Washing volume 10 3000 Ll well Resolution 1ul Incubator temperature 25 30 37 0 fixed Temperature accuracy 5 6 Temperature stability 5 6 Temperature uniformity 1 Warm up time 6 min ambient 25 to room tempera ture Display Liquid crystal display Interface RS 232 bi directional communication port External dimensions 450mm L x390mm W X 190mm H Weight 13kg Power supply a c 220V 50Hz Input power 80VA Working environment 10 C 30 C Relative humidity lt 70 Fuse T3 15AL 250V 5X20 Microplate Washer User s Manual 1 3 External structure Fig 1 1 Front view of instrument 1 ELISA plates 6 Wash bottle 3 2 Incubation cover 7 Distilled water bottle 3 Waste bottle 8 LCD screen 4 Wash bottle 1 9 Keyboard 5 Wash botile 2 10 Transparent cover Fig 1 2 Back view of the instrum
34. strument Microplate Washer User s Manual Content How to use this manual sa ss kis ias i curves a erereco a 1 Symbols on the IMSMUMEM ainiai ak i a i a etch d 2 Warning and Safety Indications ccccceesseeeeeeeeseeeeeeeeseeeeeeeeeeaaaeseeeeees 3 1 Brief introduction of the instrument cccceeesceeeeeseeeeeeeeeeneseeeeeeesees 6 1 1 Brief introduction of product is isto a i bi o a eee L ae ee ee 6 1 1 1 Name of the product Microplate Washel ccceeeeeeseeeeseeeeeneeeeneeeteeeessaeeeeseeeseaeeeaaes 6 JA22 Features Ofthe Product i a as ui ai ra NA 6 1 2 Specifications of the instrument se css soos sias a ii ii ed dees cae eee 7 Teo Exe rial SHUCUUIN G e aeae i rss S a S A a a S Aaa AEEA 8 abies A ola e e ia a ae ae a e PE 9 1 5 Principle OL THE INSLLMENT Kiske iii i kiai i aa ks Ldk i i Gr Li 10 2 Installation of the INStrUMENL cccececeseeeeeeeeeeeeeeeeeeeseeaseeeeeeeaaeees 11 21 Unpack sake i a a a Li a i ali calle a ak 11 2 2 Insiall the bottles and tubing Kisse a tensile a a eeeciaveneces 11 a A EAEAN ETE e ua aa ces A E a a a cus A a A aa Au hee a 12 2 4 tar theinstr ment cua ira saaa ia a i S O i S a a a aa a a aa ai 12 3 Settings and adjustment of the instruMeNt ceeeeeeeeeeeeeeeeeees 13 3 1 Advanced SCUINGS Ls sai sai ent ats et ctsa el te leet ete ieee ea thee 14 3 1 1 Adjust the LCD Contrast ratios 2 0 2 2issiseentiedladlaed tildhddidedidedidecedea teeta ad edi
35. tion Plate No 1 position Wash head 12 Wash head 12 Plate Flat type F Plate Flat type F Center 30 1 mm Bot wash 9 8 mm Top 4 5 mm Left 28 0 mm Bottom 15 0 mm Right 32 1 mm a Parameter interface one b Parameter interface two Fig 3 8 Screen of plate parameter settings The parameter description and the specific modification operation are stated as follows 1 Plate No Code number of plate type selected between 1 10 2 Wash head 8 12 way wash head can be selected and the default value is 12 3 Plate settings Flat type F U type U and V type V can be selected and the letters in the brackets are their code numbers The default setting is Flat type F 4 Center The centre position Corresponds to the centre position of the first strip of microwells Keys H and H are used for adjustment by 0 1mm 5 Top Position on the top Corresponds to the top plane top aspirate position of the first strip of microwells Keys and are used for adjustment by 0 1mm 6 Bottom Position at the bottom Corresponds to the bottom position liquid dispensing position of the first strip of microwells Keys and g are used for adjustment by 0 1 mm 7 Bot wash Bottom washing position Corresponds to the middle position bottom wash position in the vertical direction of the first strip of microwells Keys and H are used for adjustment by 0 1mm
36. trument works normally you must set up the system properly Note If the system settings are incorrect it may lead to the result that liquid spills over plates can not be washed effectively or wash head be damaged etc After starting the instrument at first it will initialize the systematic parameters and check if the programs need upgrading Then the system primes the pipelines for parameter setting please see Section 4 4 with preprogrammed wash liquid At this moment press CANCEL key to cancel the priming process or when the selected wash bottle has no liquid the instrument will automatically cancel the priming process and get into the main interface finally the instrument automatically gets into the main interface of the system as shown in Fig 3 1 The keys A V H can be pressed respectively to make the menu selection of upwards downwards right and left and the selected menu will be shown with highlight At the main interface pressing the START key will enter a corresponding menu procedure while pressing the CANCEL key will show the system LOGO On the LOGO interface pressing any key will return to the main interface of the system The menu names of ASPR DISP PRIME and RINSE indicates that the corresponding keys ASPR PRIME RINS on the keyboard can be used to enter the corresponding menu directly Ver1 0e SN XXXXX
37. u of system and continue with operation Switch off the system Power off please a switch off confirmed b Prompts of power off Fig 3 10 Switch off the system r Note We strongly suggest that you carry out the switch off the system program before switch off the instrument The system rinses the pipelines automatically with distilled water saves the current code number of the plate washing program for the purpose of the next use 20 Microplate Washer User s Manual 4 Operation of the instrument 4 1 Worktable and ELISA plates The waiting groove is in the middle of the worktable When the instrument at stand by status the wash head stops in the waiting groove in front of the groove is a carrying table used to place the ELISA plates Warning Only when the instrument is at stand by status the ELISA plates can be put on or taken off from the worktable otherwise some accident may take place and lead to damage to the instrument or injury to the operator Microplate Washer supports ELISA plates of specifications such as 12x8 12x4 and 8x6 etc but different wash heads may have limitations to different ELISA plates The detailed relation is as follows Wash head 8 pins 12 pins ELISA plates 12x8 Available Available 12x4 Not available Available 8x6 Available Not available When installing the ELISA plates pay attention to the direction of the ELISA plates

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