User Manual 520A
Contents
1. INTERFACE SETTINGS TEMPERATURE DIFFERENCE SET UP FACTORY DIAGNOSTICS TEMP OFFSET Chamber 1 2 C SELECT SELECT Bottle 1 1 C pH SET UP MENU Low buffer pH 4 0 SELECT High buffer pH 7 0 SELECT Temperature comp AUTO SELECT Manual temp Value 20 C SELECT LOGIN CODE TO DIAGNOSTICS Type login code to get Access a 5 HY Code init value 51761 OLLU CHAPTER 3 19 3 7 6 Normalization Parameter NORMALIZATION PARAMETER 1 In the manual Procedures for investigations of small vessel using small vessel Wire Myograph there is a description on how the nor Norm Time 60 Sec SELECT malization is done and also a description of the parameters used Before the automatic normalizations are started the normalization parameters Wire diameter 40 um SELECT must be set Release Near 1000 um SELECT Norm Time The Time setting in seconds that defines the duration of each step in the Release Far 1000 um automatic normalization Wire diameter The diameter of the wire used to mount the tissue Release Near and Release Far The pre defined distance that the jaws will move apart when RELEASE is pressed The motor run until the distance between the jaws is equal to the release value IMPORTANT BE CAREFUL NOT TO ENTER A TOO HIGH VALUE AND THEREBY STRETCH YOUR MOUNTED TISSUE TOO MUCH WHEN THE RELEASE NEAR OR RELEASE FAR BUTTON IS PRESSED NORMALI2. 520A Step 1 Involves setting up the chamber for calibration Fill the chamber to a normal level with double distilled water Move the jaws apart Press NEXT STEP IMPORTANT TURN ON THE HEAT AND SET THE TEMPERATURE AT THE LEVEL AT WHICH THE EXPERIMENTS WILL BE PERFORMED Step 2 Involves setting up the Calibration Kit for the actual force calibra tion Make sure that the pin of the T balance is placed between the wire and the jaw as illustrated in figure 3 2 Carefully move the calibration bridge until the pin of the T balance is placed freely between the wire and the jaw which means it does not touch the wire or the jaw see figure 3 2 NOTE THE WEIGHT SHOULD NOT BE PLACED ON THE T BALANCE YET Press NEXT STEP when the calibration kit has been properly placed 14 WIRE MYOGRAPH SYSTEM 520A USER MANUAL Step 5 C3 9 C9 Follow the weight calibration procedure in the User Manual Fill the chamber with double distilled water When ready go to next step NEAR CALIBRATION IB CO CO Place the calibration bridge on the myograph Place the bridge so the pin does not touch the wire jaw When ready go to next step NEXT STEP Figure 3 2 Illustration of how to fit the T balance pin between the wire and the gap in the jaw support Step 3 Initiates the heating process for the chamber In order for the cali bration to be accurate the transducers must be heated to the exper
3. EN QU NB DUM RU nnmnnn ODUN M XA EUR A 6 LA Wire interface SEI UO TTE 6 1 2 Auto Dual Wire Myograph EI MI Suite 7 Chapter 2 8 2 1L Setting Up the Wire Myograph Systemi GEM a 8 ESE TOROS calibrato mm m 9 Chapter The Wire Interface Menus cccsessseccsnnsseeceensseeceensseceenssseceonssseceoonssseneonssseeeeasseceonssseeeonsaseeeonsseeeeenssseeneasseceonsass 10 3 1 General description on how to Navigate the touch screen 10 A NE 10 God LUN I T E E N E AEAN A E D PEE 11 N a T AEEA EOE 12 P ANI E m m m E A A AA E A AN P I EE A A E A TT 12 GRONN BI II T aeei E E T fan E a EE E E 12 a eE NO e E
4. waiting for input When ALL is chosen all lines corresponding to all four channels will turn blue ALL Changing the numeric value for the chosen parameter can be done by touching UP or DOWN arrows J LY Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory ENTER Pressing the white X in the red box will exit the menu and take you automati cally to the Main Menu MULTI INTERFACE 3 2 Power up screen Multi Myograph System After turning on the Wire Interface an introduction screen appears It dis plays the product system model no software version number date andthe Model 520A system ID number Software Revision 02 04 The system is auto initializing while this screen is displayed 10 WIRE MYOGRAPH SYSTEM 520A USER MANUAL 3 3 Main Menu The Main Menu gives a good overall status on how the Wire Interface is working It displays the force pH probe temperature timer and the active status buzzer timer heat Icon for Heater ON OFF K Timer Icon for Buzzer ON OFF MAIN MENU 00 00 00 4 f ON the icon is green Near chamber force 20 2 mN Far chamber force 10 2 mN Probe temperature 36 8 pH value 6 81 TIMER ZERO HEAT BUZZER SETTING Buzzer icon This icon indicates the status on the Buzzer If the icon is grey the Buzzer is OFF If the icon
5. 23 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com salesedmt asia com supportedmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China TEO 0 215425 1330 Fax 86 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 1 678 302 7013 www dmt usa com sales dmt usa com support amp dmt usa com
6. E 13 CNN OCS CR asasina teo 13 aha Normalzation TES FELD 16 JLo PA CalLraton 9 17 3 74 Select Analog Output a n cuc E RET cond ER dU 18 gt E E ee sens E EA NA E E A AA cos pas EU 19 S LO Normalization Paramete a 20 C NAI e QNI REEL 20 Appendix 1 System specifications EE EXY REFER RR RR ERR YR HEMN RE EE ER KEEN GE VR NN RE RN REF ERN EUN KU EUN RI C E ER RID NE CRM UN 23 CONTENTS 3 SAFETY The Wire Myograph System has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be used for these purposes Nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the apparatus the internal electronics pose a risk of electric shock Do not use this apparatus near water e reduce the risk of fire or electric shock do not expose this apparatus to rain or mo
7. in the calibration formula and is obtained automatically if the Temperature com pensation has AUTO selected If MANUAL is chosen the Manual temperature is used in the pH calibration formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffers is measured with a thermometer and entered manually in the Manual temperature line The calibration procedure is listed in 4 individual steps and needs to be performed one by one to calibrate the pH Step 1 Involves cleaning the pH electrode and the temperature probe with double distilled water When ready press NEXT STEP Step 2 Place the pH electrode and temperature probe in the high buffer solution here pH 7 and turn on stirring When the relative pH output in the blue line is stable press NEXT STEP Step 3 Place the pH electrode and temperature probe in the low buffer solution here pH 4 and turn on stirring When the relative pH output in the blue line is stable press NEXT STEP Step no 83 Follow the pH calibration procedure in the User Manual Clean the pH electrode and the temperature probe When ready go to next step NEXT STEP pH CALIBRATION sevo CC Apply buffer with the High pH value to the pH and temp probe When the pH reading is stable go to next step pH readings 1863 NEXT STEP pH CALIBRATION sere Apply buffer with the Low pH value to the pH and temp probe When t
8. 4 to 70 C For Download of Firmware and connection to PC Software BNC connector at the back used to Connect to ext acquisition system Analog output range Range is 2 5Volt Technical specifications Auto Dual Wire Myograph 520A Vessel size Vessel alignment Mounting supports Chamber Chamber cover Force range Force resolution Force calibration Chamber size Chamber material Chamber temperature range Chamber heating Chamber temp Resolution Chamber temp Stability Temperature probe Micrometers One pH input pH calibration Output impedance Data on the transducer Max Range Operating temperature Frequency range Optional accessories Enable pH electrode on the Wire Interface range temp correction Electronic valve gt Manually in the X Y and Z plan Jaws One chamber Supplied 200mN On display 0 1 mN Resolution on analog BNC connector is higher than O O1mN Manual with guidens on display Standard 10ml Acid resistant stainless steel 20 45 C Built in With built in pipes for suction gassing superfusion in chamber cover 0 2 When target temperature attained External PT1000 Manually operated precision micrometers max 12mm Range pH 0 14 Manual with guidance on display 200 Ohm T 200mN 15 to 50 20Hz pH O 14 50 100 to 240 auto 50 60 Hz via external power supply APPENDIX 1
9. 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded intentional RF sources as these may interfere with the proper operation 4 WIRE MYOGRAPH SYSTEM 520A USER MANUAL UNPACKING THE WIRE MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be pursued as quickly as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Wire Myograph System Please contact DMT Sales Department for packing instructions if the original packing material is unavailable After unpacking your new Wire Myograph System please use the following list to check that the system is complete Wire Interface Power supply the shape of the AC plug varies by country be sure that the plug has the right shape for your location Wire Myograph 520A Wire Myograph connection cable with a temperature probe wo sets of stainless steel mounting jaws with supports Chamber cover Accessories 1 x calibration kit incl
10. DANISH MYO TECHNOL Ost WIRE MYOGRAPH SYSTEMS 520A USER MANUAL TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 35 are trademarks of ADInstruments Pty Ltd DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with Wire Myograph system 520A No part of this document may be reproduced by any means without the prior written permission of Danish Myo Technology A S Copyright Danish Myo Technology A S DANISA MeO TECHNOL OGS 2 WIRE MYOGRAPH SYSTEM 520A USER MANUAL CONTENTS IIo CN ERE cR PRRURDURENS 2 RIED ooo m M 2 EC Declaration COMPOCIIEY si nx ric On cR nc acc RR RN C OTRA DR d 2 Unpacking the Wire Myograph wiDO Re XE SRM ER M EN M VIA CULVR VEA FON rid wx IQ CR K KR EBEN ROC OL FER EK UR RN REMO nnna 5 Chapter 1 Wire Myograph System oVOlIVIGW asususunkexxu Sk x EE MIO EE GU EDU ME EN EN
11. PENSATION DONE BY IF THE MESSAGE OFF 15 DISPLAYED ON THE MAIN PAGE OF THE WIRE INTERFACE EVEN THOUGH THE CHAMBER IS PLUGGED IN AT THE REAR OF THE WIRE INTERFACE THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED IN ADDITION IF THE FORCE READING S APPEAR YELLOW CANNOT BE RESET TO ZERO AND THE TRANSDUCER CANNOT BE RECALIBRATED THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED Step no x7 5 Turn the heat on Wait until the temperature is stable Temperature setpoint 37 0 Probe temperature 36 8 BACK HEAT OFF NEXT STEP otep no 5 Make sure that the transducer is not subjected to any force When the relative force reading is stable go to next step Force Chamber 3261 BACK NEXT STEP CHAPTER 3 15 Step 5 At this step place the 2 gram weight at the pan on the calibration T balance closest to the transducer over the transducer see figure 3 1 to NEAR CALIBRATION simulate the stretch created by the contraction of a mounted ring prepara tion Step no ge Carefully place the 2 g weight on the pan When the relative force reading is stable go to next step Remember a 2 gram weight in a 90 vector is divided and the transducer will only detect 1 gram or 9 81 mN of force The weight placement should cause a positive increase in the four digit number Wait at least 30 to 45 seconds for the applied force to stabilize before pressing NEXT S
12. RMALIZATION Step no 1 Time 48 This is the force that the vessel is stretched successively at the stretch steps xo 0 00 uM Yo 12 13 mN e Norm Force Force 12 14 mN When all parameters are programmed start the automatic normalization Motor pos 0 00 um Pressure 0 00 kPa PAUSE NORM CHAPTER 3 21 If the normalization is successful a parameter screen is shown and the mounted artery is ready for use with the optimal pre load tension If the normalization is NOT successful an error message is shown 22 WIRE MYOGRAPH SYSTEM 520A USER MANUAL NEAR NORM RESULTS Norm Pressure 13 3 kPa Norm Factor IC1 1C100 0 9 Norm Time 60 sec Norm Force 1 5 mN Wire diameter 40um Eyepiece cal 0 50mmyvdiv Eyepiece a1 0 5 Eyepiece a2 218 F 0 98445 Xo 0 00 um Yo 37 44mN Normalized lumen Dia 397 26 um Motor pos 458 80 um NEAR NORMALIZATION Step no 2 Time 0 Xo 0 00 um Yo 48 13 mN Force 53 14 mN Not enough steps to normalize GO BACK and check parameter APPENDIX 1 SYSTEM SPECIFICATIONS Technical specifications Wire Interface Voltage Current max Dimensions Net weight Environmental humidity Environmental operating temperature Environmental storage temperature USB connector 4 Analog outputs External 100 240VAC to 24VDC ADAPTER 10 3 3Amps at 24VDC 34 x 25 x 15 cm LxDxH 5kg 20 to 80 RH none condensing 15 to 40 C
13. TEP Force Chamber 3346 BACK NEXT STEP Step 6 is to verify that the calibration was performed correctly The Force NEAR CALIBRATION Chamber reading should be 9 81 0 1 mN If the Force Chamber reading is off by more than 0 1 mN then remove the weight press BACK to returnto Step no 1 5 Step 4 and repeat the calibration process If the Force Chamber reading is The transducer is now calibrated satisfactory then press NEXT STEP to end the calibration Force read out should be 9 81 mN 0 1 mN If OK go to next step Otherwise repeat the calibration Force Chamber 9 81 mN BACK NEXT STEP NORMALIZATION RESULTS 3 7 2 Normalization results Is used to check the normalization results Select which one to check and press ENTER NEAR Norm Result FAR Norm Result SELECT The normalization results NEAR NORM RESULTS Norm Pressure 13 3 kPa Norm Factor C1 IC 100 0 9 Norm Time 60 sec Norm Force 1 5 mN Wire diameter 40 Eyepiece cal 0 50mmy div Eyepiece a1 0 5 Eyepiece a2 2 5 0 98445 0 00 um Yo 37 44mN Normalized lumen Dia 397 26 um Motor pos 458 80 um 16 WIRE MYOGRAPH SYSTEM 520A USER MANUAL CHAPTER 3 3 7 3 pH Calibration Procedure Before the pH calibration is performed be sure to select the way the pH electrode is intended to be used See pH Set up Menu under Interface Settings in chapter 3 7 5 2 The temperature is an important parameter
14. ZATION PARAMETER 2 Norm Pressure The target transmural pressure usually 13 3 kPa 100 mmHg Norm Pressure 13 3 KPa SELECT Norm Factor Norm Factor 0 9 IC100 is the internal circumference corresponding to a certain target trans mural pressure typically 13 3 kPa 100 mmHg IC1 is the normalized inter Eyepicece cal 0 40 SELECT nal circumference calculated from IC100 by multiplying it with a factor less than 1 usually O 9 Eyepiece cal The distance in mm between lines on the eyepiece 3 7 7 Mounting MOUNTING ON CHAMBER NEAR Mount the vessel using the LEFT or RIGHT arrows for either the NEAR or FAR chamber to move the jaws together or apart Near After setting the normalization parameters go to Mounting on chamber Force Near 12 1 mN near or Mounting on chamber far Actual Motor pos Near 0 RESET makes the jaws move together When the jaws touch each otherthe Wating for command motor stops and the Actual Motor pos is set to 0 00um MOTOR NEAR MANUAL RELEASE makes the jaws move apart the distance set in the Release Set 4 Value see TOGETHER makes the jaws move together until they touch each other When the jaws touch the motor stops LEFT and RIGHT arrows move the jaws manually in the given direction STOP MOTOR stops the motor 20 WIRE MYOGRAPH SYSTEM 520A USER MANUAL 3 7 7 1 Mounting step by step 1 With no wires on the jaws and with the jaws a
15. aws 4 on each side Motorized micrometer Arrow buttons for manual adjustment of the jaw position a P Ru Fle ae E Window at the bottom of the chamber F OLINE 2 under the wire jaws HE ESO Wire jaw connected to force transducer Wire jaw connected to micrometer e 2 Chamber separator e i Figure 1 4 Auto Dual Wire Myograph 520A with close up of the chamber CHAPTER 1 7 CHAPTER 2 SETTING UP 2 1 Setting up the Wire Myograph System Wire Interface rear panel Power supply 2 oc WWII YY pH Meter L Data Acquisition System via BNC cables or USB Gas supply manifold amm e 2 JL Electronic vacuum valve Vacuum Pump Suction Bottle i Auto Dual Wire Myograph Oxygen Carbogen Compressed air Figure 2 1 Example of a complete Wire Myograph System 520A Figure 2 1 is a complete set up for the Wire Myograph System 520A The set up includes optional equipment such as pH electrode for measuring pH in the buffer an electronic vacuum valve suction bottle vacuum pump and a computer and Data Acquisition System IMPORTANT TO RECORD A NOISE FREE SIGNAL IT IS IMPORTANT TO CONNECT THE WIRE INTERFACE GROUND CONNECTION TO THE DATA ACQUISITION SYSTEM S GROUND CONNECTION 1 Wire Myograph to Wire Interface connection Connect the Wire Myograph to the Wire In
16. fset TEMP OFFSET ON CHAMBER The SELECT and ALL functions are the same in this menu as for previously described menus Pressing ENTER will store the numbers in memory for future experiments 3 7 5 2 pH Set up Menu The pH calibration is a 2 point calibration In the pH set up menu the two pH values used for the 2 point pH calibration should be selected The default in the pH Calibration setup is the pH values 4 and 7 If using pH buffer with different pH values than 4 and 7 for the pH calibration enter the appropri ate pH The temperature is an important parameter in the calibration formula and is obtained automatically if the temperature compensation it is set to AUTO as shown If MANUAL is chosen a manually set temperature is used in the pH calibra tion formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffer is measured with a thermometer and entered manually in the Manual temp Value line 3 7 5 3 Factory Diagnostics Entering Factory Diagnostics will display the Login code to diagnostics window This window is only for trained technicians and used for diagnos tics and troubleshooting purposes The general user will not have access to this window Entering the proper five digit login code however will allow the trained technician access to diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer
17. g DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing UP or DOWN arrows Pressing ENTER will save the new temperature setpoint Probe temperature 36 6 To turn the heat on press ON and it will turn green The system will heat to the desired temperature setpoint In the Main Menu the heat icon turns green when the heat is on Temperature setpoint 37 0 Probe temperature 36 6 ON 3 6 Timer Alarm Menu SET TIMER The timer and buzzer are controlled from this menu Use SELECT to program the timer The timer can be programmed in the interval from O to 23 59 59 Set Timer 00 00 00 Hours minutes seconds When Set Timer is selected use the UP and DOWN arrows to program the timer Use the RIGHT arrow to move from hours minutes and seconds Press ENTER to save the programmed time imer 12 WIRE MYOGRAPH SYSTEM 520A USER MANUAL CHAPTER 3 To start the timer press ON and it will turn green SET TIMER When the timer reaches zero it automatically switches to OFF Set Timer 00 00 00 If the buzzer is turned on it will make a tone when the timer reaches zero To activate the buzzer alarm press ON and it will turn green SET TIMER In Main Menu the bell icon also turns green Set Timer 00 00 00 Q ON OFF 3 7 Settings Menu The Setting Menu contains several sub menus These sub menus include SETTINGS Force cal
18. he pH reading is stable go to next step pH readings 883 NEXT STEP 17 Step 4 The calibration is now finished The readings in the two bottom lines are the actual pH and temperature readings pH CALIBRATION sevo 2 GB The pH electrode is now calibrated if OK go to next step else repeat the calibration pH value 4 00 Probe Temp 25 0 NEXT STEP 3 7 4 Select Analog Output optional SELECT ANALOG OUTPUT The Analog Output Menu determines what will be sent to the BNC analogue Select output 1 SELECT output REC 1 REC 2 REC 3 REC 4 at the back of the Wire Interface There 4 analog outputs and each output is individually programmable Any Select output 2 SELECT change made to the analog output will affect the data sent from the Wire Interface to a data acquisition system such as AD Instruments PowerLab Select output 3 SELECT Therefore remember to check the data acquisition system s set up when a change is made The analog output is working in range of 2 5V to 2 5V Select output 4 SELECT Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up 1 SETUP Use the UP and DOWN arrow or DEFAULT to change select a new set up Output 1 Force Setting 1 2 5V 100mN Setting 1 2 5V 100mN The following parameters the Wire In
19. ibration i FORCE NORM pH RECORD Normallze parameter results CALIBRATION RESULTS CALIBRATION OUT SELECT pH calibration Record out select INTERFACE NORMALIZE MOUNTING MOUNTING Interface settings SETTINGS SET UP NEAR FAR Normalize set up Mounting near Mounting far 3 1 Force Calibration Menu Prior to shipping the Wire Myograph System has gone through two days FORCE CALIBRATION of continuous testing including final force calibrations However DMT recommends that new force calibrations are performed before starting to Force calibration near SELECT use the Wire Myograph System for the first time Force calibration far SELECT Begin the calibration procedure by pressing Force Calibration in the Set tings Menu The sub menu will list 2 chambers for calibration The calibra tion procedure is listed in 6 individual steps and needs to be performed to calibrate the system Before starting the Force calibration the following is performed e Place the calibration bridge and T balance on the Wire Myograph see figure 3 1 allowing it to be warmed up together with the Wire Myo graph Turn on the heating in the Heat sub menu to the appropriate temperature used during your experiments e Mounta 40 um stainless steel wire on the jaws connected to the force transducers 13 The 2 g weight is placed on the pan Figure 3 1 Force calibration set up showing placement of the calibration bridge and T balance for Auto Dual Wire Myograph
20. imental temperature to be used This accommodates heatinduced expansion of the electronic parts in the transducer Otherwise inaccurate readings and transducer drift may occur producing errors into the experiment Therefore now turn the heat on by pressing HEAT ON Covering the chamber with the chamber cover will expedite the heating Place the temperature probe into the chamber for the first calibration to determine when the chamber has reached the target temperature Heating will take about 20 to 30 minutes for the chamber and transducers to reach 37 C with the chamber cover in place Once the chamber are heated and have reached the target temperature press NEXT STEP Step 4 is the first step in the actual force calibration process A four digit number will be displayed in blue at the bottom of the screen If nothing has been disturbed during the heating process the zero gram or mN calibration should be stable as indicated by the four digit number Wait at least 30 to 45 seconds until the four digit number is stable before pressing NEXT STEP NOTE NORMAL OPERATING VALUES FOR THE FORCE TRANSDUCER DURING CALIBRATION SHOULD BE BETWEEN 3000 AND 3500 IF THE VALUE IS 0 A SINGLE DIGIT OR ATHREE DIGIT NUMBER THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED IF THE VALUE IS LESS THAN 2000 OR GREATER THAN 4500 BUT STILL A FOUR DIGIT NUMBER THE FORCE TRANSDUCER IS BROKEN BUT SHOULD BE ABLE TO BE REPAIRED BY A TEMPERATURE COM
21. is green the Buzzer is ON active and will make a sound when the timer reaches zero Timer The Timer is a countdown timer that can be set to maximum 24 hours If the 00 00 00 Buzzer is activated it will buzz when the timer reach zero Heat icon This icon indicates the status of the chamber heat 3 If the icon is grey the Heat is OFF If the icon is green the Heat is ON active OFF ON Four sub menus are accessible from the Main Menu screen Zero Menu Heat Menu Timer Alarm Menu Setting Menu CHAPTER 3 11 3 4 Zero Menu SET FORCE TO ZERO This menu is used to zero the output of the transducers When using a data acquisition program like LabChart by AD Instruments this feature will Near chamber force 2 36 mN reset the baseline of the chart traces without affecting the calibrations or physically changing any pre load tensions placed on the mounted vessels The channels can be changed individually by pressing SELECT or all at once by pressing ALL Pressing ENTER will execute the zero function and return the user to the Actual Force display SELECT Far chamber force 3 38 mN SELECT 3 5 Heat Menu The heating temperatures are controlled from this menu To turn the heat on or to change the temperature for the system press HEAT in the Main Temperature setpoint 37 0 C Menu The Heat Menu will be displayed allowing the user to change the system temperature as well as turn the heat on or off Pressin
22. isture Objects filled with liquids should not be placed on the apparatus Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time The Wire Interface is delivered with an external 100 240VAC to 24VDC adapter Protect the power adapter and cord from being walked on or pinched Particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped EC DECLARATION OF CONFORMITY Danish Myo Technology A S Certify and declare that the following apparatus Wire Myograph System DMT520A Restrictive use Only for laboratory use Manufactured by Danish Myo Technology A S Skejbyparken 152 8200 Aarhus N Denmark Conforms with the essential requirements of the EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2
23. part and therefore with no force on the force transducers press ZERO in the Main Menu 2 Gointo the Mounting Menu and press RESET to zero the position of the micrometers Make sure that the display shows zero force and Actual Motor pos Near shows 3 Press RELEASE or the ARROWS to move the jaws apart Use the manual controls to move them further apart Position the first wire between the two jaws and press TOGETHER The jaws will now move together and grip the wire 4 Fix one end of the wire beneath the screw and press the RELEASE or manual motor controls Place the artery in position bring the jaws together again and fix the other end of the wire beneath the screw 5 Press RELEASE or the manual motor controls and mount the second wire through the artery on the other jaw 6 When the artery is mounted on both jaws make sure that the two wires are aligned so that they will touch each other when TOGETHER is pressed f Press TOGETHER The force readings should be zero After the vessel is mounted go to Arteries Parameter to put in the dimensions of the vessel NEAR ARTERIES PARAMETER a1 Microscope eyepiece reading from the far end of the mounted segment Eyepiece a1 1 0 SELECT a2 Eyepiece a2 2 0 SELECT Microscope eyepiece reading from the near end of the mounted segment Norm Force 1 0 mN SELECT IMPORTANT MAKE SURE THE EYEPIECE CALIBRATION FACTOR HAS BEEN ENTERED IN THE NORMALIZATION PARAMETER 2 MENU NEAR NO
24. terface be selected as output the analog channels Force Near Force Far Temperature Probe Chamber Temperature pH1 Optional Digital Output 1 Digital Output 2 Digital Input OUTPUT 1 SETUP 1 Digital Input 2 Output 1 Force When the chosen parameter is selected press ENTER to save the value in the memory Setting 1 2 5V 100mN The output range is 2 5V to 2 5V The user can select the parameter value 1 2 5V 100mN that gives 2 5V and the value that gives 2 5V In this example 100mN is 2 5 and 100mN is 2 5V Selecting asymmetrical values is also valid They can be 2 5V OmN and 2 5V 100mN OBS DEFAULT REMEMBER TO PRESS ENTER TO SAVE THE LINE CHANGES IN MEMORY 18 WIRE MYOGRAPH SYSTEM 520A USER MANUAL CHAPTER 3 3 7 5 Interface Settings The Interface Settings sub menu has an additional three sub menus Temperature Difference pH Set up Factory Diagnostics 3 7 5 1 Temperature Difference offset The temperature difference function allows the user to fine tune the tem perature set point of the system Although the temperature set point for the system can be set in the Heat Menu there may be a small discrepancy between the actual temperature of the system and the defined set point The user can adjust the temperature of each chamber individually to fine tune the temperature setting so the exact temperatures are achieved in each chamber This is referred to as a temperature of
25. terface using the grey 44 25 pin connector cable The end of the cable with the temperature probe is attached to the Wire Myograph 2 Wire Interface to computer connection Data acquisition is possible either by connecting the Wire Interface directly to a computer or through a data acquisition system able to collect voltage output 0 2 5volts such as a PowerLab data acquisition system or similar system Direct computer USB connection for Labchart AD Instruments users latest version of Labchart Connect the Wire Interface to the computer with the USB cable from the back panel to the USB port Analog Data Output through a PowerLab or similar data acquistion system optional Connect the Wire Interface to the analog data acquisition system using BNC cables Connect Rec 1 Chamber 1 on the Wire Interface to Input 1 on the analog data acquisition system Rec 2 Chamber 2 to Input 2 on the analog data acqui sition system 2 and so forth Connect the analog data acquisition system to one of the USB ports on the computer 8 WIRE MYOGRAPH SYSTEM 520A USER MANUAL 3 Turn on the Power Turn on the power to the Wire Interface at the power switch and then turn on the computer Start the data acquisition pro gram on the computer and the Wire Myograph System is now ready for use 4 Gas supply Connect the pipes for gas supply on the Wire Myograph chamber cover see figure 2 2 to an adjustable gas supply using thin silicone tubing 5 Suc
26. tion connection Connect the suction pipes on the Wire Myograph chamber cover see figure 2 2 to a vacuum pump via a suction bottle and the electronic vacuum valve as illustrated in figure 2 1 6 pH electrode optional Connect the pH electrode to the pH port on the rear of the Wire Interface Suction pipes for connection 6 to vacuum pump Access for temperature probe ME Van cM DEC _ TTET ETETETT Access for reagents and buffer Figure 2 2 Chamber cover for Wire Myograph 520A 2 2 The first force calibration Prior to shipping the Wire Myograph System has gone through two days of continuous testing including final force calibration However DMT recommends that a new force calibration is performed before using the Wire Myograph System The force calibra tion procedure is described in detail in chapter 3 7 1 CHAPTER2 9 CHAPTER 3 THEWIRE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Wire Interface 3 1 General description on how to navigate the touch screen Menus on the Wire Interface are all accessible by a touch screen To access a menu simply touch the screen When a setting needs to be changed OFFSET press SELECT beside the line to be changed Chamber 1 2 C SELECT SELEC Bottle LTG SELECT The line to be modified will turn blue indicating that the Wire Interface is
27. uding bridge T balance and 2 gram weight 1 x spool of 40 um stainless steel wire 1 x tube of high vacuum grease 1 x tube of grease for linear slides 4 x spare screws for mounting of jaws 10 x extra myograph window glasses 1 x bath divider 2 x Allen keys 1 x small screwdriver 2x 40 mm funnel Optional Accessories PowerLab LabChart DMT Device Enabler Stimulator pH meter Vacuum Pump Electronic vacuum valve Waste bottle Gas supply manifold UNPACKING THE WIRE MYOGRAPH SYSTEM 5 CHAPTER 1 WIRE MYOGRAPH SYSTEM OVERVIEW 1 1 Wire Interface front and rear 88 22 mN 46 1 mN n Near chamber force Far chamber force Probe temperature OFF pH1 is not mounted Touch display Figure 1 1 Wire Interface front Analogue recorder output connection to Grounding connect to external data acquisition software optional Data Acquisition e e POWER 1 REC 2 ON OFF switch e RECA REC 3 p a pati T OL OL ol M v We Connection port for pH electrode optional 24V DC port e 4 for power e USB connection port e for computer Connection port for Wire Myograph Figure 1 2 Wire Interface rear WIRE MYOGRAPH SYSTEM 520A USER MANUAL 6 1 2 Auto Dual Wire Myograph 520A Connection to Wire Interface Allen screws for fine alignment of the j
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