Nichipet 7000【362KB】
Contents
1. For pipetting follow the forward method the way explained in this manual If it is performed in a different way it may result in inaccurate pipetting Operate push button very gently If it is quickly released it may result not only inaccurate pipetting but also deteriorated the pipette because sample liquid may be permeated into the main body Do not reuse tip that has been used once and carefully dispose used tip If tip is used repeatedly it may cause inaccurate and impure pipetting and cross contamination among samples For example if previous sample liquid is left inside tip it is mixed with new sample liquid and the new sample is contaminated by the previous one Therefore pipetting of the next sample results wrong This phenomenon is called mutual contamination of samples Do not hold pipette horizontally or upside down when there is liquid inside tip otherwise the liquid gets into the main body and the pipette may be contaminated When autoclaving do not pile pipettes on others in the autoclave or lean pipettes with a nozzle top facing down so that self load is applied on the nozzle This pipette is made of an autoclave compatible material but because of high temperature in the sterilizer there is a risk that parts subject to load will be deformed After autoclaving and drying pipette leave it until it gets completely cool before using again If the pipette is used when warm the accuracy ma2. Fig C 7 TOOT N Too il Too A 8 TUCO N Tyo N Fig D 3 Discharge 1 Touch the top of the tip to the inside of the receiving vessel Fig D 3 2 Gently depress the push button from a to b Wait about 1 second then depress to the c position The liquid is now discharged Fig D 4 5 3 Press the eject button to remove the used tip Fig D 6 N Do not touch the tip during use or after use when liquid that is harmful to the body is being used Disassembly and assembly of the airtight unit If any of the problems included in the Troubleshooting section of this manual arise use the following procedure to disassemble and inspect this unit 1 Disassembly 1 Turn the ring in the direction of the arrow and remove the casing from the handle Fig 1 Note If the connection screw is loosened tighten it with the provided wrench Fig 2 2 Remove the screw that holds the ejector and remove the ejector from the casing Fig 3 3 Remove the screw that holds the casing Fig 4 4 Lightly press the nozzle and remove the casing A set as shown in Fig 4 5 Remove each part Fig 5 2 Assembly 1 Attach each part to casing B Fig 5 2 Assemble and attach the casing A set Fig 4
3. Nichipet 7000 Multi channel digital micro pipette User s manual CERTIFIED 1SO9001 Thank you very much for purchasing Nichipet 7000 For safe and proper use of the Nichipet 7000 please be sure carefully to read this manual After reading this manual please keep it in a convenient place for quick reference BE ORIGINATIVE Always Pursuing Originality Since 1944 Nichipet 7000 Multi channel digital micro pipette Features e Complete autoclaving 121 C 20 minutes is possible with this unit e Fatigue free operation for long time e Digital system allows easy one touch volume setting e One touch locking function with single hand e Covering 0 5uL to 300uL range with 4 models each of 8 and 12 channels e Unit construction design helps to prevent accuracy change due to hand temperature e Suitable for sampling to a micro plate with 96 well and 9 mm pitch e Because the handle and casing angle can be adjusted freely 360 degrees the unit can be used at a position of your choice e Includes tip eject function The tips can be removed without touching them by fingers e The simple casing structure enables easy maintenance Standard accessories TID 8 Ch oes x 16 12 Nee x 24 e Silicone grease nn xl e Cleaning wire nosses xi e Wrench nsss xi e User s manual ee xi Please make sure that none of the above accessories are missing before using this unit Safety precautions e For safe
4. 3 Assemble and attach the ejector to the assembled casing Fig 3 4 Assemble and attach the casing set to the handle Fig 1 Note When assembling be sure to set the counter to the maximum volume range Parts will not mesh proper Note If the connection screw is loosened tighten it with the provided wrench Fig 2 Fig 1 Handle Casing Handle Ring Connection washer Connection screw Fig 2 m lt D Wrench Ejector plate holding screw Fig 3 Casing Aset Ejector button XK OD QO lt I S URS A VW Q Ag Te RB Casing holding screw Fig 4 Plunger head spring First spring Plunger holder set Plunger set X ring holder X ring Nozzle Position to gear plungears Nozzle spring Fig 5 Autoclaving Autoclaving of this complete unit is possible Follow the procedure below at a temperature of 121 C for 20 minutes 1 Be sure to loosen the lock handle and set the counter to the maximum volume number of the volume range Fig A 2 Loosen a connecting screw in the Fig 2 by 180 degree 3 Loosen four screws on the casing in the Fig 4 by 180 degree 4 When placing inside the autoclaving unit be careful not to damage the nozzle Also place in a position that does not put any weight on the nozzle 5 After autoclaving be sure to dry the unit thoroughly Drying Car
5. liquid measurement devices your pipette should be calibrated under conditions of use The calibration results obtained in your laboratory may vary from our results due to differences in environmental testing conditions NICHIRYO Tokyo office 1 10 1 Kandanishiki cho Chiyoda ku Tokyo 101 0054 Japan TEL 81 3 6273 7652 English FAX 81 3 6273 7944 URL http www nichiryo co jp E mail i sales nichiryo co jp 2013 Ver 1
6. 0 5 G 40 1 4 lt 0 5 RES Sg 407200 lt z uL 100 1 0 lt 0 4 40 200uL 200 0 9 lt 0 3 5 50 1 4 lt 0 7 NP7 12K 50 300 S E uL 150 1 0 lt 0 5 50 300uL 300 0 6 lt 0 2 Tips autoclavable Code Ger i Color Applicable model ei Q ty BMT2 UT 0 1 10 Natural NP7 8V 12V 31 5 1000 BMT2 SG 5 200 Natural NP7 8S 12S 8L 12L 53 0 1000 BMT2 K 50 300 Natural NP7 8K 12K 58 9 1000 Rack tips autoclavable Code Fe Color Applicable model Q ty BMT2 UTR 0 1 10 Natural NP7 8V 12V 960 96 x 10cases BMT2 SGR 5 200 Natural NP7 8S 12S 8L 12L 960 96 x 10cases BMT2 KR 50 300 Natural NP7 8K 12K 960 96 x 10cases RECALI BRATION PROCEDURE 1 Remove the cap by pulling it out with your fingers Fig 6 2 Loosen the lock handle by turning it counter clockwise and stop when the oval opening on the lock handle faces you Fig 7 3 Rotate the push button until one of two hex head screws can be seen through the oval opening Fig 7 4 Loosen both hex head screws with a hex head wrench 1 5 mm by turning them counterclockwise one by one Fig 8 5 Keeping the hex head wrench inserted into one hex head serew turn the push button to calibrate the pipette Fig 8 6 The pipetting volume can be adjusted by rotating the push button clockwise to increase and counter clockwise to decrease Please refer to the table standard volume adjustments Angle 0 5 10uL 5 50uL 40 200uL 50 300uL 360 0 31uL 1 6
7. and proper use of the Nichipet 7000 please be sure carefully to read the Safety precautions in this section and CAUTIONS on the next page before starting work with it e The contents of CAUTIONS are matters that require the user s attention not only for using the Nichipet 7000 properly but also for preventing the user from accidents and physical damage e After reading this manual please keep it in a convenient place for quick reference Please observe the following cautions to ensure the safe use of this product N CAUTIONS Be sure to observe the following instructions for using Nichipet 7000 properly and safely If the user misuses the Nichipet 7000 or disregards the following instructions it may result in injury to the user or and other persons or physical damage to this NOW 10 11 12 13 instrument or and other equipment 1 Do not use for any other purpose than handling of liquid 2 3 Do not use for handling of liquids that will be directly injected into the Never modify this instrument as this may cause an accident body As certain types of liquids are harmful to the body never discharge any sample liquid while pointing the instrument at a person Do not eject tips while this unit is pointed at a person Do not eject tips when there is liquid inside The sharp protruding tips of the unit are dangerous Please use care when handling Please make s
8. d Replace the X ring set with the new set No grease on the L plungers and or X rings Coat the plungers and or X rings lightly with the provided grease J a Liquid in the nozzle A If immediately after suction or if there just some adherence of liquid disassemble and clean the parts Looseness of body and casing L Ring is loose Tighten the ring Connection screw is loose XR Tighten the connection screw with the provided wrench If the problem persists even after the above points have been checked stop usage of the unit immediately and consult with the sales outlet where the unit was purchased Caution At this time please confirm that there has been no contamination by chemicals that are harmful to the human body Replacement parts list Please specify volume and channel number when ordering Spare parts Contents Size Ring x1 Common Connection washer x1 Common Connection screw x1 Common Casing A x 1 Insert nut x 5 F Ejector button x 1 Casing A set Ejector shaft A x 1 Each size Ejector shaft B x 1 Ejector spring x 1 Casing B x1 Each size Plunger fixing unit x 1 First spring x 2 Guide shaft x 2 Plunger fixing unit set Guide tube x 2 Each size E ring x 4 Bush x 1 Stem x 1 Pl
9. ndle in the lock direction and tighten Fig A Note Do not exceed the specified liquid volume limit otherwise the instrument may be damaged or deteriorate in the quality Note After changing the volume use the unit after first carrying outa trial operation V 104L L 2004L SC Ki 0 2 0 0 H 0 SN pas a Pointer mark 4 Fig A Fig B 2 Suction 1 Attach the disposable tip to the nozzle attach from the rack when doing so 2 Press the push button down to a b Fig C 3 With the push button pressed down insert the top of the tip to a point suitable for suction of the desired volume Fig D 1 4 Return the push button gently to its original position The liquid will be pulled into the tip At this time pause for 1 second to wait for complete suction of the liquid Fig D 2 5 Withdraw the tip gently from the liquid By doing this gently hardly any of the liquid will remain on the outside surface of the tip If liquid does remain remove it by paper or something similar being careful not to touch the tip s point Note Do not carry out suction from the c position shown in Fig C liquid will be pulled into the h nozzle if done from this position Note Operate the push button es gently Releasing quickly will cause liquid to be pulled into the inside of the unit _ causing possible loss of accuracy
10. ry out drying immediately after autoclaving Dry the unit thoroughly in a constant temperature air drier at 60 C for over 60 minutes 1 Dry the product in the same condition as when it was autoclaved 2 Be careful not to damage the nozzle when placing the unit in the dryer Also make sure that the unit is placed in a position that does not place any weight on the nozzle 3 Make sure that the unit turned to the normal temperature after the drying Tighten the connecting screw in the Fig 2 and four screws on the casing in the Fig 4 And make sure that a ring in the Fig 1 is tightened Note After drying if the unit is assembled while it is still warm screw head damage etc may occur causing damage to the product or loss of product performance Be sure to reassemble the unit after it has completely cooled down Also using the product while it is warm may result in loss of accuracy Do not touch the unit directly with your hand just after AN autoclaving and drying because the unit is extremely hot during autoclaving and drying Directly touching the hot unit may cause an accident Maximum Permissible Errors i Measured u Pipette types Accuracy Precision Code ee as S 1 8 0 lt 4 0 sae EE 5 4 0 lt 2 0 0 5 10uL 10 2 0 lt 1 0 NP7 8S G 5 3 0 lt 1 5 NP7 12S 25 2 0 lt 0 8 5 50uL uL 50 1 0 lt
11. uL 6 5uL 9 6uL 720 0 63uL 3 2uL 13uL 19 2uL 7 Tighten the both hex head screws after adjusting the push button and gt measure the accuracy of the pipette 8 Repeat the above procedures until the pipette is calibrated within the specified accuracy An accuracy test should be made at the specified minimum and maximum volume of i Ip each pipette cc 9 Return the cap to its original position Fig 6 Push button CD A Increase Ca B Decrease Lock handle Oval openin A I val opening U gt gt Hex head scre w Hex head wrench Fig 7 Fig 8 Troubleshooting Problem Probable cause Solution Foreign matter in the Remove foreign matter with Liquid is not L nozzle provided cleaning wire sucked in e a X ring is worn or Replace the X ring set with the ae Liquid leakage from tip damaged new set r Plunger is damaged or rusty Pa Replace the X ring set and Plunger set with the new sets Nozzle tip attachment L part is damaged Replace the nozzle set with the new set Attached tips are loose Attach the tips firmly J Push button movement is poor Plunger has shifted Press the push button a few times X ring is damage
12. unger head spring set 8ch x 8 12ch x 12 Common Plunger 8ch x 8 12ch x 12 f Plunger set Plunger head 8ch x 8 12ch x 12 Each size X ring suppressor set X ring suppressor 8ch x 2 12ch x 3 Each size Nozzle spring holder set Nozzle spring holder 8ch x 2 12ch x 3 Common X ring set X ring 8ch x 8 12ch x 12 Each size Nozzle set Nozzle 8ch x 8 12ch x 12 Each size Nozzle spring set Nozzle spring 8ch x 8 12ch x 12 Common Casing holding screw x4 Common Ejector plate x 1 ees Ejector plate holding screw x 1 Gase Wrench x1 Common Note Parts marked with a star x influence precision of the product When replacing these parts please replace all of them with new parts X Please contact your local distributor for price information X Specifications of the instruments and optional accessories as well as contents of accessory sets are subject to change without notice X No part of this manual may be reproduced reprinted in any form without permission of the copyright holder This is strictly prohibited by the copyright law Inspection and Calibration Statement The enclosed pipette was tested and calibrated under closely controlled environmental conditions to ensure that it meets published calibration specifications The precision and accuracy results obtained for this pipette are provided on the enclosed calibration certificate Because temperature and humidity conditions affect the calibration results of
13. ure that the tips are securely attached to the nozzle Scattering of liquid will occur if tips fall off the nozzle If liquid that is harmful to the body has splashed onto the unit treat it properly before using it When using liquid that is harmful to the body never touch a tip that has been used Do not use this unit to mix liquid etc This will cause tips to loosen fall off or result in liquid splashing on the unit After autoclaving or drying do not touch the unit as it is very hot Touching the unit could result in bodily harm Although this product is designed to resist chemicals it may be damaged by some kinds of chemicals such as N methyl pyrrolidinone etc Please contact the manufacturer before using special chemicals CAUTIONS to dispose pipettes or tips Please follow local low or ordinance when pipettes or tips are disposed 10 11 OC NOTE Users are required to strictly observe the following points in order for the instrument to keep its excellent precision reproducibility and original performance for a long time Do not expose pipette directly to the sun when working with it or for 2 hours before starting work otherwise the pipette may lose accuracy Avoid working with pipettes in a humid and hot place Just before starting work with pipette avoid touching tip and nozzle cylinder as far as conditions are allowed If nozzle cylinder is warmed by your hand accuracy may vary
14. y not come up to the standard level After autoclaving and drying pipette assemble the pipette after it is completely cooled if it is assembled when it is still hot it may cause deterioration in the pipette such as breakage of the screw threads When rotating push button do not exceed the specified sample volume limit otherwise pipette may be damaged or deteriorated Do not perform pipetting with less liquid than set volume If the quantity of liquid is less than the set volume it may cause the liquid to scatter into the main body and the pipette may deteriorate in quality Contents _ Operating procedure Pe ee RER RRRREEE RER RR KRIER 5 Disassembling Reassembling the airtight chamber eneen 9 Autoclaving Drying the pipette eee eee ee e 17 Specification ee RER RR RR RRE ERE RER e 18 Calibration procedure Pe ee ee 19 Troubleshooting Ce RER RR RREREREERRRRREREREKEE RER RR RRERERE RER 21 Parts list Pe ee EEE 23 Operation 1 Volume setting 1 Turn the lock handle to the unlock direction and loosen it Fig A 2 Turn the push button to set the digital counter to the desired volume When increasing volume first set the scale a half revolution further and then set it to the desired amount When decreasing the volume the scale can be set directly to the desired amount At this time set the counter s scale to the pointer mark located at the bottom section of the counter window Fig B 3 After volume setting turn the lock ha
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