Pipet-Lite - Mettler Toledo
Contents
1. OI 0 d ert f E amp Limited Warranty See the Limited Warranty and Limitations of Liability Statement Please complete and return the Warranty Registration Card on receipt of your pipette RAININ pipettes are calibrated with RAININ tips To assure excellent reproducibility and performance use only RAININ tips as recom mended in this manual Specified performance is guaranteed only when RAININ tips are used Contacting RAININ Technical Information T 800 543 4030 F 510 564 1617 tech support rainin com Pipette Service T 800 662 7027 F 781 935 7631 service rainin com Direct Order Line T 800 472 4646 F 510 564 1617 pipets rainin com RAININ website www rainin com From outside North America T 1 510 564 1600 global rainin com from outside the U S METTLER TOLEDO Offices METTLER TOLEDO website www mt com rainin Mettler Toledo Schweiz GmbH Im Langacher 8606 Greifensee CH Verkau 044 944 45 45 salesRN ch mt com Service 044 944 47 47 serviceRN ch mt com Mettler Toledo GmbH Ockerweg 3 35396 Giessen DE Verkau 49 641 507 222 info mtd mt com Service 49 641 507 307 info mtd mt com Mettler Toledo SAS 18 20 avenue de la P pini re 78220 Viroflay FR Ventes 0130971717 marcom fr mt com Laboratoire d Etalonnage Accr dit 264 rue Abraham Lincoln Service Pipette 62400 Bethune FR Service 032. Example volumes for the 200 uL model are shown below note the intermediate setting at the right KMA ol FA 67uL 108 uL 157 6 uL Volume ranges and increments are shown below Pipet Lite Range pL Increment Volume Adjustable Recommended pL 2 uL 0to2 0 1 to 2 0 002 10 pL 0 to 10 0 5 to 10 0 02 20 pL 0 to 20 2 to 20 0 02 100 pL 0 to 100 10 to 100 0 2 200 pL 0 to 200 20 to 200 0 2 300 pL 0 to 300 30 to 300 0 5 1000 pL 0 to 1 000 100 to 1 000 2 0 2000 pL 0 to 2 000 200 to 2 000 2 0 5000 pL 0 to 5 000 500 to 5 000 5 0 10 mL 0 to 10 mL 1 mL to 10 mL 20 0 20 mL 0 to 20 mL 2 mL to 20 mL 20 0 Filter Pipet Lite 5000 uL 10 mL and 20 mL pipettes use a filter in the end of the shaft to help prevent liquid entering the shaft and contaminating the piston should the plunger snap up during aspiration Using such a filter is particularly important when pipetting large volumes Replace the filter if it gets wet END OF END OF END OF 5000 pL SHAFT 10 mL SHAFT 20 mL SHAFT SS Ho H i FILTER FILTER Figure 2 Filter Orientation The 5000 uL and 10 mL pipettes use the same filter oriented as shown in the diagram above 5000 pL insert the small diameter into the shaft 10 mL insert the large diameter into the shaft Filter part numbers are 6190 164 pack of 100 and 6190 165 pack of 1000 The filter for 20 mL is a cylinder Filter part numbers 6190 221 pack of
3. Pipet Lite TOME gees eh eeh Sa E 13 Pipet Lite 20ML nien yie mereen nae iaae nia 13 Contents of package Pipet Lite as ordered Sample tips Instruction Manual Rainin Test Report Performance Assurance Brochure If any item is missing call Customer Service 800 472 4646 Rainin Pipet Lite LTS are registered trademarks and LiteTouch Hang Ups are trademarks of Rainin Instrument LLC Pipet Lite pipettes are manufactured under U S Patent Nos 5 614 153 5 700 959 and 5 849 248 For use under U S patents 6 168 761 B1 6 171 553 B1 and D426 643 Introduction Pipet Lite is an air displacement pipette which incorporates major ergonomic improvements to reduce the risk of repeti tive strain injury and pain low force seals and lower spring forces contribute to a lighter feel A magnet is used to help sense and hold the piston in the Zero position which reduces static force on the operator s hand In the models with LTS shafts the patented LiteTouch Tip Ejection System reduces total pipetting forces by up to 70 Plunger button Volume lock volume adjustment Tip ejector button Digital volume indicator 4 high impact low thermal conductivity polymer quick release b Es Tip ejector arm Stainless steel tip ejector arm Shaft Disposable tip Figure 1 Pipet Lite 200 pL Setting Volume 1 Turn the volume lock counter clockwise to the position shown at
4. internal components to corrosive fumes can be reduced by using RAININ tips with aerosol barrier filters 10 Replacement Parts see pages 12 13 Legend for Pipet Lite 2 pL to 2000 pL A Plunger Button B Shaft Coupling C Shaft D Tip Ejector E Piston Assembly F Seal G O ring H Stroke Spring J Seal Retainer L Series note the second set of A and B shaded are for the new handle Pipet Lite 2007 L 2 L 10 L 20 L 100 L 200 L 300 L 1000 L 2000 6202 055 6202 056 6202 057 6202 058 6202 059 6202 306 6202 060 6202 192 6202 500 6202 501 6202 502 6202 504 6202 505 6202 506 6202 507 6202 509 6202 062 6202 062 6202 062 6202 062 6202 062 6202 062 6202 062 n a 6202 499 6202 499 6202 499 6202 499 6202 499 6202 499 6202 499 n a 6202 063 6202 064 6202 065 6202 066 6202 067 6202 425 6202 068 6202 214 6202 071 6202 071 6202 071 6202 073 6202 073 6200 419 6202 074 6200 168 6202 076 6202 077 6202 078 6202 079 6202 080 6202 427 6202 081 6202 082 6200 131 6200 138 6200 143 6200 150 6200 154 6200 415 6200 161 6200 166 6200 132 6200 139 6200 170 6200 151 6200 155 6200 414 6200 162 6200 167 6202 083 6202 083 6202 083 6202 084 6202 084 6202 084 n a n a ejrjo mnj mj olo wv 6200 196 6200 196 6200 198 6200 201 6200 200 6200 416 n a n a SL Series note the second set of A and B sh
5. 100 and 6190 222 pack of 500 Tip Selection and Mounting Always use RAININ tips with Pipet Lite pipettes Pipet Lite pipettes are calibrated with RAININ tips and performance to published specifications can only be guaranteed when RAIN IN tips are used To mount a tip press the Pipet Lite shaft into the end of the tip with light force The tip will seal properly on the shaft with minimal force do not use more force than is required Tip Immersion Depth The recommended depth for tip insertion into the sample for each Pipet Lite volume is shown below Pipet Lite Volume Range Immersion Depth 2 uL 0 1 2 uL 1 2 mm 10 uL 0 5 10 uL 1 2 mm 20 uL 2 20 uL 2 3 mm 100 uL 10 100 uL 2 3 mm 200 uL 20 200 uL 3 6 mm 300 uL 30 300 uL 3 6 mm 1000 pL 100 1000 uL 3 6 mm 2000 uL 200 2000 UL 3 6mm 5000 uL 500 5000 pL 6 10mm 10 mL l mL 10mL 6 10mm 20 mL 2 mL 20 mL 6 10mm Tip immersion depth is critical If these depths are exceeded the volume measured may be inaccurate possibly out of specification Tip angle is also important Hold the pipette within 20 degrees of vertical Operation Before pipetting valuable samples it is a good idea to prac tice aspirating and dispensing water before pipetting with actual samples 1 Set the desired volume as described on page 2 2 Attach a new RAININ tip Press the shaft into the tip with only sufficient force to make a good seal 3 Press the plunger button to th
6. 21 64 54 66 marcom fr mt com N V Mettler Toledo S A Leuvensesteenweg 384 1932 Zaventem BE Sales 32 2334 0211 general mtb mt com Service 32 2334 0211 general mtb mt com Mettler Toledo S A E Miguel Hern ndez 69 71 08908 L Hospitalet de Llobregat Barcelona ES Ventas 0034 93 223 7666 mtemkt mt com Servicio T cnico 0034 93 223 7666 mtemkt mt com Mettler Toledo A S Naverland 8 2600 Glostrup DK Salg 43 27 08 25 info mtdk mt com Service 43 27 08 25 info mtdk mt com Rainin Instrument LLC 7500 Edgewater Drive Box 2160 Oakland CA 94621 0060 a METTLER TOLEDO Company Prices and specifications are subject to change without notice Copyright 2007 Rainin Instrument LLC 9920 237 Rev M
7. 6202 525 C 6200 373 6200 374 6202 298 6200 373 6200 374 D 6202 215 6202 216 6202 296 6202 215 6202 216 E 6200 363 6200 369 6202 299 6200 363 6200 369 F G H CH 6200 365 6200 371 6202 301 6200 365 6200 371 6200 364 6200 370 6202 300 6200 364 6200 370 6202 222 6202 223 6202 302 6200 362 6200 368 hese parts are autoclavable C tip ejector arm H shaff Common parts for 5000 pL 10 mL and 20 mL Tube of grease 6100 555 Filters for 5000 pL 10 mL 6190 164 100 6190 165 1000 Filters for 20 mL 6190 221 100 6190 222 500 11 Pipet Lite 2 amp 10 pL Pipet Lite 20 pL A Legend for Pipet Lite 2 pL to 2000 pL Models this page and top of next page A Plunger Button B Shaft Coupling C Shaft D Tip Ejector E Piston Assembly F Seal G O ring H Stroke Spring J Seal Retainer 12 Pipet Lite 1000 pL TP A LJ on m III Wm L Pe Pipet Lite 2000 pL FP A RS Legend for Pipet Lite 5000 pL 10 mL and 20 mL below A Plunger Button C Tip Ejector Arm D Piston Assembly E Piston O ring F Cylinder G Cylinder O ring H Shaft Pipet Lite 5000 pL amp 10 mL Pipet Lite 20 mL A
8. left below so the volume setting mechanism is unlocked and free to turn LOCKED Volume is locked when wings are straight across 2 With the mechanism unlocked orient Pipet Lite so you are looking at the digital volume indicator then rotate the plunger button to change volume counter clockwise to increase and clockwise to decrease volume The volume indicator is read from the top down 2 ul 10 pl 20 pl 100 pl 200 ul 200 1 1000 pl 2000 pl 5000 pl 10 ml 20 ml 4 fo 4 fo fal jo 1 4 HK 2 z 2 7 12 2 7 2 2 7 2 5 5 5 5 5 5 5 5 5 5 5 1 25 pl 7 5 pl 12 5 ul 75 pl 125 pl 225 pl 0 75 ml 1 25 mill 4 25 ml 7 5 ml 12 5 ml Red digits Black digits 2 20 uL Black uL Red tenths hundredths of uL 100 300 UL All digits black whole yL 1000 5000 uL Red mL Black tenths hundredths of mL 10 mL Red mL Black tenths of mL 20 mL Red mL Black tenths of mL 3 To eliminate errors due to mechanical backlash when set ting the desired volume first turn the knob 4 turn above the desired volume Then turn the knob slowly clockwise until the desired volume is displayed Always dial down to the desired volume 4 Turn the volume lock clockwise see diagram above to prevent accidental changes to the volume setting
9. tip to the original sample container if desired Discard the used tip Pipetting Liquids of Varying Density Pipet Lite lets you compensate for solutions of density much different from water by setting the volume slightly higher or lower than that required The compensation amount must be determined empirically For example if pipetting 10 uL of CsCl solution you deter mine that the volume delivered is actually 8 5 uL 25 sam ples Change the volume setting to 11 8 uL and repeat the measurements If the volumes delivered are still not close enough to 10 uL make another slight volume adjustment until the measurements are as desired Temperature Considerations Warm or cold liquids can be measured with good precision by using a consistent pipetting rhythm This will help mini mize any differences in heating or cooling effects within the pipette Use a new tip each time for best accuracy and precision when measuring samples with temperatures greatly different from ambient and do not pre rinse As with any air displace ment pipette you will get best results if there is no delay between picking up the sample and dispensing it Autoclaving Autoclavable parts of Pipet Lite are the shaft and the tip ejector 121 C 1 bar 15 20 minutes Do not autoclave the complete pipette or any parts other than the shaft and the tip ejector Tip Ejector Arm Removal Three types of tip ejector are used and all types can be removed with min
10. RAININ Pipetting 360 Pipet Lite Magnetic Assist Pipette continuously adjustable digital microliter pipettes 11 pipettes for volume ranges from 0 1 pL to 20 mL EDO Table of Contents NMOCUCTION 2 sedcs sce ahh ouaa ea a Setting Volet EEEEENENENEENEE ENEE EE 2 FUNGI tee a cient are S 3 Tip Selection and MOuNnting ccccccceeeceseeteteeeneeees 3 Tip Immersion Depp 4 ODO ON pieces E 4 Pipetting Guidelmes 5 Pre Rinsing Recommended e 5 Reverse Mode Pipetting cccccceecceeeeeeeseeeteeeeeeees 5 Pipetting Liquids of Varying Dee 6 Temperature Considerations 6 AUTOCIOVING 20 3 EE ee EENS 6 Tip Ejector Arm Removal l enn eeren 7 Pipet Lite Storage enrere 7 Troubleshooting and Repairs c 8 Service Calibration and Report 9 Specifications sennnnneeenniiseeniriiseeeririrnerrrrrrrrerrrrrns 10 Replacement Parts cccccceceeeeesesetessssstseeaaaees 11 Contacting RAININ Back Page Figures Figure 1 Pipet Lite Pipette 2 0 Figure 2 Filter Ortentofion 3 Figure 3 Removing the Tip Ejector Am 7 Line Drawings Pipet Lite SCH ege Gegen ege 12 Pipet Lite TO He acs Sha ties weaned ae e e ots 12 Pipet Lite QO IL e stage a a 12 Pipet Lit TOQ seed E antes tree 12 Pipet Lite 200 UL c 12 Pipet Lite 300 UL oo eeeececceteeetteeeettereeeteees 12 Pipet Lite 1000 UL sisisi 13 Pipet Lite 2000 UL eree 13 Pipet Lite 5000 UL eeng a ass 13
11. aded are for the new handle Pipet Lite 2007 SL 2 SL 10 SL 20 SL 100 SL 200 SL 300 SL 1000 SL 2000 6202 109 6202 110 6202 111 6202 112 6202 113 6202 426 6202 114 6202 115 6202 516 6202 517 6202 518 6202 519 6202 520 6202 521 6202 522 6202 523 6202 062 6202 062 6202 062 6202 062 6202 062 6202 062 6202 062 n a 6202 515 6202 515 6202 515 6202 515 6202 515 6202 515 6202 515 n a 6200 134 6200 140 6200 145 6200 147 6200 157 6200 413 6200 160 6200 169 6200 133 6200 133 6200 144 6200 148 6200 156 6200 419 6200 163 6200 168 6202 183 6202 184 6202 185 6202 079 6202 080 6202 427 6202 081 6202 082 6200 131 6200 138 6200 143 6200 150 6200 154 6200 415 6200 161 6200 166 6200 132 6200 139 6200 170 6200 151 6200 155 6200 414 6200 162 6200 167 6202 083 6202 083 6202 083 6202 084 6202 084 6202 084 mio n a 6200 196 6200 196 6200 198 6200 201 6200 200 6200 416 n a n a j jzomm o o lo 5 hese parts are autoclavable C shaft D tip ejector arm Legend for Pipet Lite 5000 pL 10 mL and 20 mL A Plunger Button C Tip Ejector D Piston Assembly E Piston O ring F Cylinder G Cylinder O ring H Shaft L Series SL Series L 5000 L 10ML L 20ML SL 5000 SL 10ML A 6202 193 6202 194 6202 297 6202 217 6202 218 A 6202 510 6202 511 6202 512 6202 524
12. e FIRST STOP and hold if in this position The magnetic assist will help you sense and hold this position 4 Holding Pipet Lite vertically place the tip into the sample to the proper depth and relax your thumb pressure on the plunger The light piston spring will move the piston upward aspirating sample Do not let go of the plunger button or the piston may snap up quickly resulting in inaccurate measurement 5 Pause briefly longer for macrovolume pipettes to ensure that the full volume of sample is drawn into the tip 6 Withdraw the tip from the sample If any liquid remains on the outside of the tip wipe it carefully with a lint free tis sue taking care not to touch the tip orifice Dispensing 1 Touch the tip end against the side wall of the receiving vessel and press the plunger slowly past the FIRST STOP to blowout bottom of stroke Wait 1 second for 2 300 uL volumes 1 2 seconds for 1000 uL and larger Longer for viscous solutions 2 Still holding the plunger withdraw the tip sliding it along the wall of the vessel Release the plunger 3 Press the tip ejector button lightly to discard the tip Use a new tip for each sample to prevent carry over Repeat for the next pipetting cycle Pipetting Guidelines Pipet Lite pipettes incorporate several new features which enhance pipetting consistency You should also maintain 1 Consistent pickup and dispense rhythm 2 Consistent speed and smoothnes
13. er s specifications should be used as guide lines when establishing your own performance specification Pipet Lite Specifications Volume Increment Accuracy Precision Model uL uL uL yL lt 2 pL 0 2 0 002 12 0 0 024 6 0 0 012 1 0 2 7 0 027 1 3 0 013 2 0 1 5 0 030 0 7 0 014 10 pL 1 0 0 02 2 5 0 025 1 2 0 012 5 0 1 5 0 075 0 6 0 03 10 0 1 0 DI 0 4 0 04 20 pL 2 0 02 7 5 0 15 2 0 0 04 10 1 5 0 15 0 5 0 05 20 1 0 0 2 0 3 0 06 100 pL 10 0 2 3 5 0 35 1 0 0 1 50 0 8 0 4 0 24 0 12 100 0 8 0 8 0 15 0 15 200 pL 20 0 2 2 5 0 5 1 0 0 2 100 08 08 0 25 0 25 200 0 8 1 6 0 15 0 3 300 pL 30 0 5 2 5 0 75 1 0 0 3 150 0 8 1 2 0 25 0 375 300 08 2 4 0 15 0 45 1000 pL 100 2 3 0 3 0 0 6 0 6 500 08 40 0 2 1 0 1000 08 80 0 15 1 5 2000 uL 200 2 3 0 6 0 0 6 1 2 1000 08 80 0 2 2 0 2000 0 8 16 0 0 12 24 5000 pL 500 5 24 12 0 0 6 3 0 2500 0 6 15 0 0 2 5 0 5000 0 6 30 0 0 16 8 0 10mL mt 20 5 0 50 0 0 6 6 0 5 mL 1 0 50 0 0 2 10 0 10 mL 0 6 60 0 0 16 16 0 20mL 2mL 20 5 0 100 0 0 6 12 0 10 mL 1 0 100 0 0 2 20 0 20 mL 0 6 120 0 0 16 32 0 Specifications are subject to change without notice Acids and Corrosives After pipetting concentrated acids or highly corrosive solu tions disassemble Pipet Lite and inspect and clean the piston assembly shaft and seal with distilled water Dry all components thoroughly and reassemble Extensive contact with corrosive fumes may result in pre mature seal wear and damage to the piston Exposure of
14. er to the appropriate drawing on page 12 or 13 On pipettes up to 1000 uL unscrew the shaft coupling B and remove the shaft C For 2000 uL unscrew the shaft For 5000 uL 10 mL and 20 mL unscrew the lower part of the shaft Note the way the spring seal and O ring fit on the piston Inspect the seal assembly and piston for contamination The piston should be shiny and free of corrosion Clean with distilled water or isopropyl alcohol Dry with a lint free tissue and reassemble after inspecting the interior of the shaft for any contamination For 5000 uL 10 mL and 20 mL models apply a small amount of grease to the seal If piston corrosion or staining is evident do not use the instrument Call 800 662 7027 for Service Leaks Inaccuracy Abnormal Stroke 1 2 Loose shaft Tighten coupling by hand Split or cracked shaft Remove the tip ejector and inspect the shaft Replace the shaft if necessary If the shaft was dropped remove it to see if the piston is bent If so return the instrument for service Worn seal and or o ring All models up to 2000 uL incorporate a polyethylene seal and o ring Examine the seal and o ring replacing them as necessary Pull off the old seal and o ring position the new seal and o ring on the piston assembly as shown in the drawings on pages 12 13 and reassemble the pipette Note It is NOT necessary to recalibrate Pipet Lite after changing seals Do not lubricate any component
15. imum effort do not use force For models up to 2000 pL press in the quick release tabs on the ejector arm and pull the ejector down For 5000 pL and 10 mL models grasp the top of the ejector arm and pull outward then downward For the 20 mL model pull off the lower part of the ejector arm the upper part stays in place Replace by aligning the rod with the hole in the upper part and pressing firmly To replace the ejector arm except 20 mL insert the shaft through the large opening align the top with the tip ejector pushrod and push until the ejector arm snaps in place UP TO 2000 uL 5000 uL 10 mL 20 mL Figure 3 Removing the Tip Ejector Arm Pipet Lite Storage After use store the pipette in a clean safe place Pipet Lite is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation Three types of hanger are available to hold your Pipet Lite conveniently when not in use CR 7 Free standing carousel holds seven pipettes HU M3 Set of three individual magnetic Hang Ups for mounting on ferrous surfaces HU S3 Three Hang Ups attached fo a clamp which fits onto a shelf Troubleshooting and Repairs WARNING When removing the shaft from the pipette body make sure the spring seal and o ring do not fall off the piston espe cially on the smaller models Sample Splash liquid inside the mechanism 1 Remove the tip ejector arm Also ref
16. s Pipet Lite uses a dry sealing system Except 5000uL 10 mL and L 20ML models These pipettes require a small amount of grease to be applied to the o ring to make the seal Service Calibration and Repair RAININ Pipette Repair and Calibration facilities It is recommended to use only genuine RAININ replacement parts such as seals o rings and shafts It is NOT necessary to recali brate the pipette after changing the seal o ring or shaft Recali bration of the pipette is only necessary when the piston is replaced and should be done only by qualified factory trained personnel in one of the above mentioned facilities For pipettes under warranty please note that the war ranty will be voided if the pipette has been damaged as a result of physical or chemical abuse or if the pipette has been repaired or recalibrated by any ser vice facility which is not authorized by Rainin In the US call 800 543 4030 for service Or visit the web www rainin com service asp Service is also available outside the US See the RAININ website home page www rainin com and click on the link Customers outside North America METTLER TOLEDO Pipette Repair and Calibration facilities or authorized RAININ distributors who provide service can be found by following this link and specifying the country or geographical region Contact Technical Support at 800 543 4030 for further informa tion Specifications These manufactur
17. s when pipetting 3 Consistent pressure on the plunger button at the FIRST STOP 4 Consistent immersion depth See table on page 4 5 Pipette vertically or within 20 of vertical 6 Don t invert or lay the pipette flat with liquid in the tip Pre Rinsing Recommended Some solutions may leave a film on the inside tip wall This film remains relatively constant in successive pipettings with the same tip so excellent precision can be obtained by re filling the tip and using the refilled volume as the sample Successive samples from this same tip will exhibit good reproducibility Reverse Mode Pipetting Another way of reducing error due to film retention especially useful for more viscous liquids is reverse mode pipetting The operating sequence is reversed 1 Mount a disposable tip on the pipette shaft 2 Press the pushbutton fully to the SECOND STOP 3 Immerse the tip in liquid and allow the button to return slowly to the fully UP position Wait a moment for the liq uid column to reach equilibrium in the tip 4 Wipe any excess liquid from the outside of the tip without touching the orifice 5 To dispense rest the end of the tip against the vessel wall and press the plunger to the first stop Hold this position a few seconds or long enough for the liquid column to reach equilibrium again 6 Remove the tip from the receiving vessel without blowing out the remaining liquid 7 Return excess sample in the
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