Data File - GE Healthcare Life Sciences
Contents
1. Accessories SWIFT 1000 applications software 80 2110 00 requires serial interface adapter lead M anual 2 position cell changer 10 50 mm cell holder Water heated cell holder requires circulation bath Peltier heated cell holder 80 2109 04 80 2109 05 80 2109 06 80 2109 07 requires Temperature Controller Temperature Controller 25 30 37 C Fitting kit for external sample delivery 80 2109 01 80 2109 08 requires pump and 10mm flowcell Test tube holder Spare 10 mm single cell holder Printer stand Spare Ultrospec 1000 User manual Custom Serial Interface Adapter Lead Chart Recorder Lead 80 2109 33 80 2109 09 80 2109 96 80 2109 12 80 2109 02 80 2109 03 10 50 mm cell holder The Ultrospec 1OOOE is a version of the Ultrospec 1000 which has the basic measurement modes only absorbance transmission factor concentration time intervals and wavescan no method storage and no output to printer facility EUROPE O 01 68 66 25 0 0800 515313 0761 4903 0 01 424 92 00 0 1 69 35 67 00 CN 09 8520 74 00 63 89 23 10 D 01 802 8150 018 16 40 00 93 589 05 45 0800 73 888 O 02 27322 1 x 45 16 24 00 0165 580 400 USA 1 800 526 3593 JAPAN 03 3492 9481 INTERNATIONAL ASIA PACIFIC 852 811 8693 EASTERN EUROPE 43 1 982 3826 MIDDLE EAST 30 1 9600 687 92 LATIN AMERICA 55 11 872 6833 Amersham Biosciences Biochrom Ltd2. rubber membrane which is very hard wearing The instrument is easy to use with function select entry soft keys on the keypad F1 F2 and F3 being situated directly below the corresponding option on thedisplay On the absorbance page for example concise help text is available at the push of the F1 key whereas menu and Set up are accessed by F2 and F3 respectively On other displays the function of the keys change but this is clearly indicated for example Fl and F3 act as return and accept options on parameter entry displays Pressing the red stop key acts as an escape mechanism in most situations A rapid operation short form card is supplied and this can be attached to the top of the instrument for reference if required Absorbance and Transmission M odes A sample absorbance or transmittance measurement is so easy simply key in wavelength directly via the A button on the keypad insert reference and press the set reference key then insert sample and read the result Factor Concentration M ode Entry to the Factor mode presents wavelength and factor parameter entry displays the conversion factor is used to obtain a concentration value directly from an absorbance reading After setting reference samples are inserted as required and the corresponding concentration is shown Wavescan M ode A plot of absorbance against wavelength spectrum can be Output to a chart recorder via the analogue output for permanent
3. measuring a sample entry to Set up using the F3 function key provides the opportunity to store the parameters currently loaded as a method This option is password protected and up to 9 methods can be saved A stored method can be enabled as an option directly on the instrument menu so that it is possible for an operator to switch the Ultrospec 1000 on and have a specified method available straight away Easy to clean sample compartment area Ultrospec 1000 is supplied complete with a single cell holder which can accommodate standard 10 mm pathlength cells disposable glass or UV silica a range of other accessories is also available Since the sample compartment area is actually part of the accessory cleaning it after a messy experiment is exceptionally easy simply pull it out and wipe it clean Instrument Set up pages Pressing the F3 function key on the Absorbance page provides access to basic information such as lamp usage hours and EPROM version an option to recalibrate is also available It is then possible for authorised personnel to go further via a password protected routine to customise the Ultrospec 1000 menu by disabling menu options including stored methods which are not currently required Facilities for lamp control parameters setting display contrast and enabling serial output are also present Presentation of results The Ultrospec 1000 has a multi purpose output connector on the rear pane
4. Certificate No 890333 Technical Specification Wavelength range M onochromator Wavelength calibration Spectral bandwidth Wavelength accuracy Wavelength reproducibility Light sources D etector Photometric range Photometric linearity Photometric reproducibility Stray Light Stability N oise Analogue output Digital output Dimensions Weight Power requirements 200 900nm Plane grating with 1200 lines mm automatic upon switch on 5nm 2nm 0 5nm tungsten halogen and deuterium arc single solid state silicon photodiode 0 300 to 3 000A 0 01 to 99999 concentration units 0 1 to 200 T 0 5 or 0 005A to 2 000A at 546nm whichever is the greater 0 5 of absorbance value to 2 000A at 546nm typically lt 0 2 T at 220nm using Nal and lt 0 2 T at 340nm using NaNO2 according to ANSI ASTM E387 72 0 002A h at OA after warm up 0 001A near OA and 0 002A near 2A at 600nm 100 mV per 1 000A via interface lead Centronics parallel not bi directional as standard 9 pin serial via interface lead 300 x 400 x 190 mm 6 kg 90 265 V 50 60 Hz 100 VA Specifications are measured after the instrument has warmed up at a constant ambient temperature and are typical of a production unit As part of our policy of continuous development we reserve the right to alter specifications without notice Ordering Information Ultrospec 1000 80 2109 10 Ultospec 1000E 80 2109 00 For any additiona
5. ULTROSPEC 1000 Data File UV Visible Spectrophotometer Basic measurement modes coupled with enhanced software functionality Customisation of instrument menu to suit user needs User defined method storage Multiple language software Compact reliable and easy to use The Ultrospec 1000 UV Visible Spectrophotometer is an easy to use instrument optimised not only for the teaching laboratory but also for industrial utility QC and environmental testing situations In addition to the basic modes of Operation the instrument has enhanced software and method storage functionality A laboratory technician or supervisor can customise the Ultrospec 1000 for students and operators respectively by enabling only the menu options which are required The Ultrospec 1000 will output alphanumeric text directly to a standard parallel printer not bi directional it can also be connected to a PC if required using a custom serial interface lead so that results can be further processed or electronically archived The UV lamp can be switched off in the event that the required measurements are in the visible region only in addition lamp replacement is extremely easy The instrument is delivered with a single cell holder a range of accessories including a 50 mm long pathlength cell holder is available 80 2108 68 Features W ide range of software modes Customisation of menu with password protected set up Def
6. inition of 9 stored methods M odern intuitive and easy to use Flexible function soft keys Easy lamp replacement W ide range of accessories ISO 9001 and CE Benefits Instrument can be used in many situations now and in the future as laboratory needs change M odes not currently required can be disabled by a supervisor Ease of use in QC and production line situations Will appeal to the new generation of users On screen prompts make instrument easy to use Low service cost since users can do it themselves Use in a wide range of sample handling situations and applications Quality manufacture using an approved system Amersham Biosciences Ullreapec boos LA di ie Set pri Time Intervals M ode Simple kinetics studies for teaching laboratory experiments can be readily performed The wavelength of interest is entered together with the time interval at which absorbances are to be read between 1 and 60 seconds the option of having a reference reading prior to the run is available A count down facility indicates the time remaining until the next measurement Data can be output continuously via the analogue output to chart recorder or to PC with the appropriate interface Display and Keypad The back lit liquid crystal display has large characters which are easily visible useful if a group of students are gathered around for a demonstration for example The keypad isa spill proof
7. l and will output alphanumeric results via a Centronics cable to a standard parallel printer not bi directional if connected The time intervals and scan modes will output to a chart recorder if the appropriate interface lead is used The use of the custom serial interface cable means that results can be downloaded to a PC and pasted into a spreadsheet application This in turn can be ultimately downloaded to a Laboratory Information M anagement System LIM S for project manufacture archiving purposes if required A Windows based applications software package comprising modules for Wavelength Scanning Enzyme Kinetics and Quantification is also available SWIFT 1000 Summary of Ultrospec 1000 software modes Basic M easurement M odes Absorbance and Transmission M ake basic sample measurements Factor concentration Calculate concentration from a known factor Time intervals Use for simple kinetics studies Wavescan Record a sample spectrum Enhanced Software Functionality Standard concentration Compare sample concentrations with that of a known standard Standard curve Store multi point calibration curves in memory for subsequent use with samples Reaction rate Apply a factor to an absorbance change over a specified time interval for use with reagent test kits M ulti Wavelength equations Use absorbance values in equations specified by you for direct output of results saving post run calculation M ethod storage Store up to 9 me
8. l information call your local Amersham Biosciences representative or contact us on the Internet Internet http www amershambiosciences com Amersham Biosciences 06 98 Issue B D esigned and produced by Bene Imprimatur O1223 839444
9. mation when an appropriate factor defined in the reagent kit protocol is applied These experiments are readily performed on the Ultrospec 1000 by entering the wavelength delay start and end times and the factor using the display window function key combinations in Reaction Rate mode The required result of absorbance change multiplied by the factor is displayed at the end of the experiment A correlation coefficient is also calculated from 10 equally spaced absorbance time points during the course of the experiment and can be printed out with the result if required Multi Wavelength Equation Entry M ode Absorbance measurements at specific wavelengths and combination of these with appropriate factors is a means of overcoming interference effects in several applications By using the equation entry facility of the Ultrospec 1000 post measurement calculations can be done automatically the individual absorbance values and the final result of the computation are displayed and printed out if required The parameter entry window enables entry of equation operators factors and absorbance at wavelength values in any sequence making it very easy for a supervisor to input a specific equation and then save it as a method for use by an operator elsewhere This is a very powerful facility for the busy industrial QC or environmental testing laboratory Method Storage After defining parameters in any of the modes and prior to
10. record or the data viewed on the display absorbance values only The start and end wavelengths are entered and a reference scan is run to store a baseline The sample is then scanned with real time output to display or chart recorder data can also be output directly to PC with the appropriate interface Standard Concentration M ode After selecting wavelength in the Standard Concentration mode the absorbance of a known standard is measured this concentration is entered using the appropriate function key and the corresponding factor calculated This is used in subsequent sample measurements to obtain a concentration value relative to the standard and will be of use in QC laboratories Standard Curve M ode After selecting the curve fit linear regression or cubic spline and wavelength required the number of standards to be used together with their concentrations is defined The absorbance for each is measured in sequence and the software constructs a concentration absorbance calibration curve which can be used to derive the concentrations of Samples measured against it An example is the determination of protein using a standard protocol Reaction Rate M ode Reagent test kits are routinely used for the enzymatic determination of compounds in food beverage and clinical laboratories by measuring NAD NADH conversion at 340 nm The change in absorbance over a specified time period can be used to provide useful infor
11. thods in any combination of the above modes in memory for ease of use Customisation of menu The customisation of the Ultrospec 1000 menu to suit laboratory needs is an important benefit of the instrument This facility is password protected so that only authorised personnel are able to set up or change the instrument In a teaching laboratory for example a laboratory technician might choose to have only the modes of Absorbance Factor Concentration and Time Intervals available In the QC lab the supervisor may choose to have Absorbance Standard Concentration M ulti point Standard Curve entry and Reaction Rate slope calculation Similarly a production line might have Absorbance and method 1 and method 2 as the instrument start up in this case methods 1 and 2 could both be multi wavelength equations involving factors and set up in the analytical laboratory for subsequent use by the process operators The advantages of customisation and the ability to conform with standard operating procedures make Ultrospec 1000 the instrument of choice for routine use in many environments Simple lamp change The lamp assembly has been designed to make lamp changing very easy There is no need to remove the instrument top cover simply unscrew and remove the lamp cover at the back of the instrument slide out the lamp assembly and replace with a new one No lamp alignment is necessary as the lamps are prealigned during manufacture
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