FLEX-LOGIC CORRELATOR USER MANUAL
Contents
1. 19 WON NE 23 1 13 1 11 23 ae 1 311111111100 23 1111111111111111111 5 1 0 0 24 APPENDIX 22222222 25 Dynamic light scattering photon correlation spectroscopy Error Bookmark not defined Basic ideas Of a correlator 6 300 Error Bookmark not defined 8 6 0 8 2 2 2 022222222222 2 22. 27 1 INTRODUCTION Overview The correlator FC is a multipurpose real time device for auto and cross correlation measurements built on the software configurable flex logic integrated circuits A capability of further software upgrade of hardware functions is the unique feature of the FC correlator The FC is a suitable device for photon correlation spectroscopy because it has all necessary modes of operation and is a fully hardware standalone device Therefore it is possible during accumulation of the correlation function to use the computer for other purposes e g temperature control The FC has TTL input levels as standard Both autocorrelation and cross correlation functions can be measured The living display of a probability distribution of the real input signal favors correct result making The FC provides most efficient data collection in a wide dynamic range of the input signal Specifications Hardware structure Programmable flex logic Processing algorithm Hardware updated through software Processing 32 bits Input frequency 100 MHz Capacity of each channel hardware 48 bits Minimum sample time true real time operation 10 ns Input signals TTL Operation mode Autocorrelation cross correlation Time scale Multiple tau linear Multiple tau scale Number of channels 288 Configuration 36 sections x 8 chan
3. 66 6 ma eS r r oe tTt p PL a a eee npn 0 82041 4 S60 10 0 900 10 Input intensity Cops Temperature Monitor window Temperature Monitor window display current temperature in graphical form For activation of the window Temperature Monitor it is necessary to open menu View and select Temperature Monitor Temperature C 200 400 BOO n 1000 1200 Time Window for saved files Saved correlation functions can be displayed in corresponding window This window appears after open of file from menu File or clicking the file open button Name of window corresponds to name of open file ZF OPAL1 txt ic Cc orrelation F unction 10 107 Time Control window Control window is used to set parameters of experiment For activation of the Control window it is necessary to open the View menu and select one of the items in the Control menu or simply click Control button on the toolbar Correlator tab Channel Spacing Two radio buttons Channel Spacing serves to switch Multiple tau or Linea
4. 3 33 1 19 6 7 Redding WON 77 77 6 lt 7 COUNT Rate Monitor WINDOW 1 0 8 Count Rate Distribution WINKOW 0 9 8 Tempa se 111 1110 9 1 9 3 3 111111111 11 9 Control 1 1111 1 0 10 1 2 1 11111131 9 10 111111111 113113111 0 00 11 Angl TaD 1 1111111 13 2 EAE A 90 14 Cycle TiD 7 0 777 7 14 GOR 1 252 0 7 7 1 2 15 10 1111111110101 15 33 111 009 909 16 STS a sec 111111111 1111 1 01 18 TOO 2 111111 1 1 18 1 15 ev 3 Pn 19 Macro functions of the FC program 2220
5. expand the Universal Serial Bus controllers list double click the USB Serial Converter A device switch to the Advanced tab clear the Load VCP checkbox click OK now you need to perform the same actions for the USB Serial Converter B device Note In future if you connect the FC correlator to another USB port of the computer you will need to perform step 4 again 5 Installation of the FC correlator is completed Now you may start working with the FC software 3 DESCRIPTION OF FC PROGRAM Typical FC window A user may combine a suitable configuration of the FC windows on the screen Example of a screen is shown below Ed Photocor FC Control x File View Tools Macro Window Help gt Run O Reset E Stop mp Read Ld Myf Ovnals gt Control 2 gt p Run SM 3 weet 0 D ox Correlator Constants Angle Temperature Cycle 150 Channel 9 Constant temperature Temperature trend Temperature C Initial temperature C Final temperature C Trend C min Current temperature C 26 97 150 200 250 300 Time s Time For Help press F1 Angle 90 00 Temperature 27 03 Intensity 81443 Elapsed Time 150 2 Accum Time 66 8 Coherence 0 4 There are the following types of windows 1 To display measured values Accumulation Reading Count Rate Monitor Temperature Monitor File window to show saved correlation function 2 To cont
6. button executes the same command as Print from menu File Run starts the process of accumulation of the correlation function in the correlator The button executes the same command as Run from menu Tools Stop stops the process of accumulation of the correlation function The button executes the same command as Stop from menu Tools Reset resets the correlator i e sets all channels of the correlator to zero Thus the correlator is ready for a new measurement If the data accumulated was not preliminary saved before Reset it will be completely lost The button executes the same command as Reset from menu Toools Read reads out the current value of the correlation function into the computer This correlation function may be fitted saved and so forth Reading out of the correlation function into the computer does not interfere into the accumulation process Therefore repeated reading of the correlation function without stopping the process of accumulation is available The button executes the same command as Read from menu Tools DynaLS export measured correlation function to DynaLS software Control activates Control window The button executes the same command as Control from menu View FC status bar The status bar of the program is placed in the bottom of the main FC window The status bar contains six indicators Angle 34 50 Temperature 1 68 Intensity Elapsed Time 1680 0 Accum Time 0 0 Coherence U Angle shows curr
7. 14 2101 x1 Correlator Constants Angle Temperature Cycle Cycle Units for data filtration Number of 3 Duration of unit 2 Delay between rung z f 0 Number of units Status of cycle Duration of run 3 Data filtration Spike tolerance 75 f 0 Corect units Standard deviation Repeat data filtration andard deviation 72 The user has two options to assign duration of each run 2 To write desirable duration of measurement in the Duration of unit field and write 1 in the Number of units field In this case duration of run will be equal to duration of unit and possible following data filtration will be not available Desirable duration of measurement can be divided on many short units with duration of each unit written in the Duration of unit field and number of these units written in the Number of units field Obtained array of correlation function can be analyzed with the Data filtration procedure This procedure allows indicating the bad units and creating the summarized correlation function without these bad units Data filtration procedure based on well known fact that light scattering intensity on seldom large particles of contaminations dust particles considerably increases the average light scattering Threshold for indication of the bad units can be assigned in Spike tolerance field Correctness of the data filtration procedure can be estimated with corresponding value
8. Time s Count Rate Monitor window Count Rate Monitor window displays current count rate of input signals of correlator coming from photon counting system Take into account that count rate of input signals will be measured if the Count Rate Monitor window was open before Run of the correlator For activation of the Count Rate Monitor window it is necessary to open menu View and click Count Rate Monitor Count rate history can be saved in common file of results by clicking button Save 50 100 150 200 z250 300 350 Time Count Rate Distribution window Count Rate Distribution window displays current differential probability distribution of input signals of correlator coming from photon counting system Take into account that probability distribution of input signals will be measured if the Count Rate Monitor window was open before Run of the correlator For activation of the Count Rate Distribution window it is necessary to open menu View and click Count Rate Distribution Count Rate Distribution can be saved in common file of results by clicking button Save Bf Count Rate Monitor 2 Ioj x ee mm ee 111111111 111111 1 111 1111 111111 1111111111114 1 39 3 kh ulungia bemm
9. for temperature control set temp R set the temperature in the Temperature filed on the Temperature tab in the Control window The process of temperature control starts immediately read tx 0 read the SetTemperature txt file from the folder where the Photocor exe file located At every command execution the next line from the SetTemperature txt file will be read Then the value from this string will be entered to the Temperature field on the Temperature tab in the Control window and temperature control starting usage example see Macro termol phm file Using this command it is impossible to read any string from the Set Temperature txt file Main functions to work with the measurement file open win file conf 0 open the File report setup window set file index N set the file index in the Index field in File report setup window Take into account that file index while saving to the disk of the computer will be converted to five digit format i e if you enter 1 as file index file name will be Test00001 txt save 0 save the standard file of the measurement with txt extension File name and file index correspond to the name assigned in the File report setup window After execution of this command the value of the file index in the Index field in File report setup window will be increased by 1 Take into account that before saving the data to files it is necessary to read the correlation function to the computer using the f
10. the file The name of the file is a combination of a prefix and an index 3 Folder for saving is specified in Folder edit string 16 4 Click OK After auto indexation has been adjusted to save a protocol file simply open File menu and choose Save Auto or click Save on the toolbar Print Prints a document Print Preview TeeChart Print Preview IOl x Printer EPSON Stylus Photo R220 5 7 Setup Print Orientation Portrait Landscape Reset Margins View Margins Proportional Smooth After all settings are completed check how the data is arranged on pages before printing For this purpose open File menu and select Print Preview Your file will be displayed on the screen in the way as it will be printed Click Print in TeeChart Print Preview window Print Setup Properties Ready EPSOM Stylus Photo R220 Seres Comment Orientation Size 4 210 297 mm Source Sheet Help Network Cancel Portrait Landscape Before printing make sure that parameters of pages are set correctly For this purpose open File menu and select Print Setup An appropriate window will appear 17 View menu View Tools Macro Window Help contol o Constants Accumulation Angle Reading l Temperature Count Rate Monitor F Cycle Count Aate Distribution Temperature Monitor w To
11. FLEX LOGIC CORRELATOR FC USER MANUAL CONTENTS 77111111 555511111111111 10 00 1 1 INTRODUCTION 222222222 2 2 2 CV 1 1 1 11 2 OSIM LNG eee acces 2 823 3 E 71110 2 2 8 680 3 SYSTEM 3 3 3 3 33313133131311311 1131 1 1 111 1 1 1 0 0901 3 2 3 3 3 3 1 1 11 3 installation of the 8 1 2 7 11 101 3 3 DESCRIPTION OF FC PROGRAM 2 2222222222 6 TB CAFC
12. Light Beating Spectroscopy Plenum Press N Y 1974 B J Berne and R Pecora Dynamic Light Scattering with Applications to Chemistry Biology and Physics Willey Interscience N Y 1976 H Z Cummins and E R Pike eds Photon Correlation Spectroscopy and Velocimetry Plenum Press N Y 1977 V Degiorgio M Corti and M Giglio eds Light Scattering in Liquids and Macromolecular Solutions Plenum Press N Y 1980 S H Chen B Chu and R Nossal eds Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems Plenum Press N Y 1981 B E Dahneke ed Measurement of Suspended Particles by Quasi Elastic Light Scattering John Wiley amp Sons N Y 1983 R Pecora ed Dynamic Light Scattering Applications of Photon Correlation Spectroscopy Plenum Press N Y 1985 K S Schmitz An Introduction to Dynamic Light Scattering by Macromolecules Academic 1990 W Brown ed Dynamic Light Scattering the Method and Some Applications Clarendon Press Oxford 1993 E R Pike and J B Abbiss eds Light Scattering and Photon Correlation Spectroscopy Kluwer Academic Publishers 1997 LK Yudin M A Anisimov V A Agayan V I Kosov G L Nikolaenko J Sengers A compact photon correlation spectrometer for research and education Int J Thermophys 18 pp 1237 1248 1997 27
13. abeled A and B on the rear panel of the FC correlator for photon counting system with single PMT module only one input A or B is connecting 4 After all cables is connected additional configuration of FC correlator are required open the Device Manager for Windows XP click the Start button click the Control Panel click Switch to Classic View in the upper left area of the window click the System shortcut switch to the Hardware tab click the Device Manager button for Windows Vista 7 click the Start button click the Control Panel click the System and Security shortcut only for Windows 7 click the Device Manager in the category Sytem File Action View Help H Sound video and game controllers H ie System devices Universal Serial Bus controllers Intel R ICH9 Family USB Universal Host Controller 2934 Intel R ICH9 Family USB Universal Host Controller 2935 Intel R ICH9 Family USB Universal Host Controller 2936 Intel R ICH9 Family USB Universal Host Controller 2937 Intel R ICH9 Family USB Universal Host Controller 2938 Intel R ICH9 Family USB Universal Host Controller 2939 Intel R ICH9 Family USB2 Enhanced Host Controller 293A Intel R ICH9 Family USB2 Enhanced Host Controller 293C USE Composite Device USB Root Hub USB Root Hub USB Root Hub USB Root Hub USB Root Hub USB Root Hub USB Root Hub
14. an choose a solvent from the Solvent list box and Viscosity of a solvent will be calculated automatically according to the set temperature The data for solvents are stored in text files with cns extension These files should be in the same directory as Photocor exe file Similar files for any solvents can be created in the same manner if necessary 21011 Corelator Constants Angle Temperature Cycle Solvent water Temperature C 2 Viscosity cF 1 008 Corect viscosity for temperature Refractive index 1 33285 Wavelength nm 532 8 Angle degree 36 77 7 Experimental parameters setting Determination of a particle size from the characteristic decay time of a measured autocorrelation function is based on the Stokes Einstein formula see p 21 of this Guide Therefore before data processing it is necessary to let the program know the values of the experiment conditions represented in the formula They should be set in the Control Constants window It is necessary to set four values in this menu They are temperature solvent viscosity solvent refractive index and laser wavelength 11 To free the user from the searching of solvent viscosity and refractive index for the given temperature and wavelength through the books and special tables the FC software includes corresponding files for most widely used solvents To use this possibility just set the appropriate solvent in the Solvent toggle field of
15. ent angle Temperature shows current temperature Intensity shows the current average intensity during accumulation Elapsed Time time elapse since run Accumulation time shows effective time of accumulation of the correlation function Coherence shows a ratio of the maximum ordinate of the exponential correlation function to the base line value 24 APPENDIX A DYNAMIC LIGHT SCATTERING PHOTON CORRELATION SPECTROSCOPY Basic ideas of a correlator The FC correlator is a powerful device for the measurements of time dependent correlation functions The cross correlation function Gi of two signals light scattering intensity for example t and h t describes the similarity of the signals in the time domain This function is defined as G12 t lt h t h t t gt where 7 is delay time and the angle brackets denote an average over the time t The autocorrelation function describes the correlation between a signal and a delayed version of itself of a signal 4 t Gi t lt h t h t 7 gt The correlator s algorithm consists of a multiplication of one signal with another that has been shifted delayed in time and then taking the integral of the product over time The FC is designed for operation with the photon correlation spectroscopy PCS technique Briefly it can be explained as follows the laser beam is scattered by the particles or macromolecules suspended in a liquid and undergoing Brownian motion T
16. he scattered light spectrum is broadened due to the various phase shifts The half width of the scattered light spectrum is given by the formula T Dg where D is the linear diffusion coefficient of the Brownian particles and g is the modulus of the scattering wave vector For a monodisperse suspension the diffusion coefficient D is related to the particle hydrodynamic radius R by the Stokes Einstein equation D kT 6nnR where k is the Boltzman constant T is the absolute temperature n is the shear viscosity of the solvent The autocorrelation function of the scattered light field in a normalized form is Tt gi t e The autocorrelation function of the light scattering intensity is the fold of the Fourier transform of the spectrum with itself and has the normalized form 2Tt go 1 g 1 e The decay time is determined by the particle size In the case of a mixture of particles with different sizes can obtain gilt JGMe dr The scattered light is received by a photodetector and the output signal of the detector is processed by the digital correlator to obtain the autocorrelation function A computer calculates the mean size or 25 size distribution of the suspended particles from the autocorrelation function by inversion of the relation given above 26 RECOMMENDED LITERATURE B Chu Laser Light Scattering Academic Press N Y 1974 H Z Cummins and E R Pike eds Photon Correlation and
17. le from Angle tab in the Control window to the file with pcs extension save _end 0 write the end of line symbol to the file with pcs extension Used for pcs file formatting function in non active if Control window is closed 22 Window menu Window Help Cascade Tile Horizontally Tile Vertically wo Accumulation 2 Count Rate Monitor 1 3 Control 4 Reading 5 Temperature Monitor E Macrot T OPAL tet Cascade Arranges windows in an overlapped fashion Tile Horizontally Tile Vertically Arranges windows in non overlapped tiles lri Oe ti Goes to specified window Help menu Help Help Topics About Photocar Help Topics Displays the help file About FC Displays the version number of application FC toolbar The toolbar is placed on the main FC window It duplicates executing some functions of the menu bar To execute a required operation or to choose any option simply click the necessary button Toolbar aaa x ry A gt Run Stop Reset mh Read Aig Dynal s SE Contra E Run SM Open opens the Open dialog box for opening a saved FC file The button executes the same command as Open from the File menu 23 Save saves the current results into a file with presetting made in the File setup window The button executes the same command as Save Auto from File menu Print opens the Report Printing window for printing a hard copy of the measurement protocol The
18. nels factor 2 Number of monitor channels 72 Linear time scale mode Number of channels 128 Number of monitor channels 3 Software dust cutoff regime Yes Prescaling Not required Living monitor of probability distribution Hardware Design USB interface Dimensions LxWxH 160 x 95 x 35 mm Power requirements 5V x 0 2A 2 INSTALLATION System requirements FC correlator requires the following minimum system configuration Microsoft Windows 2000 XP Vista 7 PC compatible computer Spare USB port 30 MB free hard disk space Installation of the FC software Before installing the FC correlator you need to install the FC software 1 Insert Photocor CD into the CD ROM drive 2 Open the Software folder on the Photocor CD double click the FC 6 0E exe file and follow the on screen instructions to complete the installation Note You must have administrative privileges in order to install FC software on Windows 2000 XP Vista Important To run DynaLS after installation you are to have Aladdin HASP key inserted in the parallel or USB port of your computer Without HASP user may run DynaLS during evaluation period with limited number of loadable data files Installation of the FC correlator GY y Rear panel of the FC correlator 1 Connect the USB cable to the connector labeled USB on the rear panel of the FC correlator 2 Connect the other end of the cable to the USB port on the computer 3 Connect cables from photon counting system to inputs l
19. of angle during turning is shown in Current angle degree string Clicking of Stop button interrupt current angle operation 13 Temperature tab Temperature tab allows to control temperature of thermostat with accuracy 0 1 C 2101 x1 Comelator Constants Angle Temperature Cycle Temperature trend Temperature C 20 Initial temperature C Final temperature C Trend Cmn Curent temperature FC 20 Monitor reset There are two modes of temperature control Constant temperature mode allows to write desirable value in Temperature spinner controls After click of Run button the temperature will go this temperature When thermostat reaches this temperature it will be stabilized with accuracy 0 1 C Temperature trend mode allows to assign desirable temperature trend in range of Initial temperature to Final temperature with assigned Trend This operation starts after click of Set button Running value of temperature is shown in Current temperature string After click of Set button the temperature history will shown in Temperature monitor window during all time To clear Temperature monitor window click Monitor reset button Cycle tab 1 Cycle tab allows to run simple cycle of measurement The Number of runs field allows to assign desirable number of measurements Waiting time between each run is written in Delay between runs field Duration of each run can be assigned in frame Unit for data filtration
20. oggle Any changes in tab Constants will be activated after clicking Set button 12 Angle tab Stepper motor driven turntable allows automatically to set a scattering angle with resolution 0 01 2101 x1 Corelator Constants Angle Temperature Cycle Angular step Angle degree 136 77 Initial angle degree i277 W Verification Angular step degree Current angle degree 136 77 There are two modes of turntable control Constant angle mode allows to write desirable value in Angle degree spinner controls After click of Set button this angle will be set Check the box Verification means that each angle will be set after verification procedure which consist in turning of arm to certain angle 150 After that the arm of turntable will go to the chosen angle We recommend use this procedure seldom for example if scattering angle was set manually with existing knob in the warm gear in this case turntable will have wrong angle scale After single verification procedure please uncheck the box Verification Angular step mode allows to write desirable value of angle in Initial angle degree spinner controls and desirable value of angle increment in Angular step degree spinner controls Each click of Set button will change angle from initial value with angle increment equal to chosen step Sign minus corresponds to decrement of angle changing with clicking of Set button Running value
21. olbar w Status Bar thar Save options Control Opens Control window in appropriate tab Accumulation Reading Count Rate Monitor Count Rate Distribution Temperature Monitor Opens appropriate window Tools menu Tools Macro Window Biur Stop Reset Read DynaLlS Hardware Loading Run starts the process of accumulation of the correlation function in the correlator Stop stops the process of accumulation of the correlation function Reset resets the correlator i e sets all channels of the correlator to zero Thus the correlator is ready for a new measurement If the data accumulated was not preliminary saved before Reset it will be completely lost Read reads out the current value of the correlation function into the computer This correlation function may be fitted saved and so forth Reading out of the correlation function into the computer does not interfere into the accumulation process Therefore repeated reading of the correlation function without stopping the process of accumulation is available DynaLS export measured correlation function to DynaLS software 18 Macro menu FC software supports Microsoft Visual Basic Scripting This means you can write powerful macros that automate your measurements Macro program examples can be found in Macro subdirectory of the main application C Program Files FC Macro by default Macro Window Help EC GotProd Win32 PhCor e Aun Macro Alt F9 ave heade
22. ollowing command read cor 0 Additional functions to work with the measurement file 21 These functions allows to create file with custom selected data save header 0 Create file with pcs extension which defined in the File report setup window In this case the file index entered in the corresponding filed in the File report setup window will be ignored and the 000 always used as file index for the pcs files Impossible to assign a custom file index for the pcs files Important this command is required to use other commands from the following list save time 0 write the system time when the correlation function was red from correlator to the memory of computer save temp 0 write the latest temperature read from the temperature controller or the Temperature string from Constants tab in Control window if controller are switched on save_int 0 write the current measured intensity and the current value of coherence to the file with pcs extension For crosscorrelation mode AB or BA is selected the sum of the intensities from the both channels will be saved save coherence 0 write the current coherence to the file with pcs extension save_conc 0 write the value of Concentration in the File report setup window to the file with pcs extension save _phd 0 write the value of intensity monitor to the file with extension only if intensity monitor switched on save_ang 0 write the value of the Ang
23. ple time order of magnitude in the Correlator tab of the Control window It is necessary to switch correlator to the linear mode before using this command Range of N values is 9 to 1 read st 0 read the SetTime txt file from the folder where the Photocor exe file located At every command execution the next line from the Set Time txt file will be read Then the value from this string will be entered to the Sample time field on the Correlator tab in the Control window usage example see Macro st1 phm file Using this command it is impossible to read any string from the Set Time txt file Before using this command it is necessary to switch the correlator to the linear mode Functions for the turntable control 20 set angle R set the angle on the turntable wset angle N the checking procedure of the angle setup finishing will be performed for the N seconds If the angle setup is finished before N seconds the next command of the macro file will be executed immediately read ang 0 read the Setangle txt file from the folder where the Photocor exe file located At every command execution the next line from the Setangle txt file will be read Then the value from this string will be entered to the Angle field on the Angle tab in the Control window After that the angle will be set on the turntable usage example see Macro Indik1 phm file Using this command impossible to read any string from the Setangle txt file Functions
24. r 0 eset aco Dim Check Counter Intens Check True Counter U Mew Chri h Do While Counter lt 14 Oper read ang U E dit Undo delay 10 ee reset Corpo ee tle ti Cirle run cor 0j Run 5M Copy Ctrl C delay 10 Paste SS 0 save 0 Delete Del save time QO Save As Select all Etrit Save _cone 0 Save Find Ctrl F save int 0 Find Next F3 0 Counter Counter 1 Replace Ctrl H The FC macro functions on the Macro menu allow one to create save and execute a macro file A macro file contains the FC macro language commands that instruct the FC correlator in accordance with the desirable consequence of steps These commands can cause a message to be displayed control and start sub processes set global variables etc All manual operations which are available to a user can be performed by executing the appropriate macro command or running the macro file Macro functions of the FC program Note Argument of function must be a needed variable inside the parenthesis For functions without variables argument must be 0 Argument of function can be the integer N or the real R For some functions the variables N or R have restricted range Main functions for measurement control run_cor 0 start the correlation function accumulation the Accumulation window will be active after executing these command read co
25. r 0 read the correlation function from correlator to computer the Reading window will be active after executing these command stop cor 0 19 stop the correlation function accumulation the Accumulation window will be active after executing these command reset cor 0 reset of the correlator the Accumulation window will be active after executing these command delay N delay for N seconds If N 1 the function will wait for run _cycle 0 command to be complete update 0 updating the data on the Constants tab in the Control window run cycle 0 start the cycle with parameters defined on the Cycle tab in the Control window After finish of the cycle procedure the Data filtration performs Then the results are saved in the file with txt extension The file name index defined in the File report setup window After execution of this command the value of the file index in the Index field in File report setup window will be increased by 1 Functions for the correlator setting set spacing N if N is O the linear mode set if N is 1 the Multiple tau set get_st 0 return the current value of the Sample time usage example set_spacing 0 a get_st 0 MsgBox Sample time amp a set _ stm N set the the Sample time mantissa in the Correlator tab of the Control window It is necessary to switch correlator to the linear mode before using this command Range of N values is 1 to 99 set ste N set the Sam
26. r hardware configuration of the correlator If Linear channel spacing is set the correlation function is accumulated and displayed in the linear time scale If Multiple tau channel spacing is set the correlation function is accumulated and displayed in the quasi logarithmic time scale The change of the mode must be confirmed with clicking Set button 15 x Correlator Constants Angle Temperature Cycle Channel spacing Inputs Multiple tau Linear Autocorrelation 2 8 Sample time Crosscorrelation Number of channels 286 10 Operation mode The FC correlator has two functionally equal inputs For autocorrelation measurements input A or B can be chosen in accordance with the physically used input of the correlator For cross correlation AB or BA modes are available which corresponds to current A x delayed B or current B x delayed A multiplication of signals Sample time setting This setting is active for Linear channel spacing only Two spinner controls are used to set value of Sample time one is to set mantissas another one is to set an exponent value of Sample Time Any changes in tab Correlator will be activated after clicking Set button Constants tab The edit strings Temperature Viscosity Refractive index Wavelength and Angle allow to set correspondingly the values of temperature viscosity refractive index of the solvent laser wave length and scattering angle A user c
27. rol correlator and some functions of the BC 1 instrument Control window with tabs File setup 3 Standard dialog boxes of Windows Accumulation window Accumulation window monitors in real time the process of accumulation of the correlation function in the correlator For activation of the window Accumulation it is necessary to open menu View and select Accumulation Depending on the hardware configuration of the correlator multiple tau or linear channel spacing the correlation function shows in form a and b correspondingly EE Accumulation W Accumulation 2 D 2 i D 5 O 0 50 100 150 200 250 20 40 60 80 100 120 Channel Channel a b Reading window Reading window shows the correlation function read out from the correlator to PC for fitting or Saving To be able to perform fitting of the accumulated correlation function you should first copy it into the computer memory using the Read button It is not necessary to stop the accumulation before reading of the correlation function Therefore you can make current estimation of the particle size during the process of accumulation For activation of the Reading window it is necessary to open the View menu and select Reading or simply click Read button on the toolbar Examples of Reading windows for multiple tau and linear hardware configurations are shown below Reading Reading 200 4010 6040 800 1010 1240 Time s
28. s shown in Correct units and Standard deviation fields For the last run of the cycle the Data filtration procedure cab be repeated many times Repeat data filtration button for example with the various spike tolerance values This option is suitable for fine tuning of the data filtration procedure to find the optimal spike tolerance for the certain sample under investigation FC title bar The title bar displays the name of the application and current active window FC menu bar Ed Photocor FC Macro File View Tools Macro Window Help 15 File menu File View Tools Macro Window Help Oper Chl 0 Save s Save Aubo Chrl 5 File setup Print Ctr P Print Preview Print Setup Exit 1 Reading 2 Accumulation 3 ORAL bt 4 C Program Files Aeading Open Opens an existing file Use this command to show and fit the saved correlation function again Save As Save the current file to a specified file name Save Auto Save with auto indexation File Setup x Falder ata File name Pretix Lates Index 20 Concentratior fo Comment FC allows assigning file names automatically To adjust the auto indexation mode follow the steps 1 Open File menu and select File setup File report setup window will appear 2 Specify a new file name into Prefix edit string Specify the starting index into the Index edit string The index is automatically incremented after each saving of
29. the Control Constants window After that solvent viscosity and refractive index will be determined automatically for the preset temperature If the solvent you are using is not in the list or if you prefer to set the constants manually just set this field to no guess Solvent file creating You can create the corresponding file yourself The extension of the file should be cns and the file should be placed in the FC software directory It should be an ordinary text file and you can create it with any text editor The format of the file is very simple An example of a table file follows this is just the file toluene cns file np 1 4969 dn dT 5 67e 4 dn d1 0 0 T eta 20 0 0 989 10 0 0 863 0 0 0 770 10 0 0 667 20 0 0 584 30 0 0 517 40 0 0 469 60 0 0 381 80 0 0 318 100 0 0 269 The first string of the file contents the following values Np is a value of refractive index at t 20 C and for Na D line wavelength dn dT is a refractive index temperature decrement 1 C dn dl is a refractive index wavelength decrement 1 A All the other strings of the file are the table of temperature dependence of solvent viscosity where T is temperature in Celsius and eta is viscosity in cP The program calculates the value of a viscosity using the linear interpolation of the closest points of the table The points should be ordered by the increasing of a temperature The file name will be included the corresponding list of the Solvent t
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