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1. Each line defines the characteristics for a sequence and the DOC10s software module can generate three types of sequences wait sequences stimulation sequences and output sequences Sequences follow one another The whole protocol can be repeated To create a sequence click the Insert icon button After the icon is O clicked a new line is created and highlighted in blue and the default values for the sequence are shown DOC10s H Start Time Type Comment Duration s Channel Perodime Widthipe 00 00 00 Wak Sequence description sequence description 00 00 00 The next step consists in defining the sequence and its configuration For each sequence a new line is created and each line is divided in eight columns Information concerning sequence s configuration is displayed in each column There can be a maximum of 99 sequences During an experimentation which requires a stimulator you can for example create stimulation and wait sequences November 28 2007 Edition 2 8 NOTOCORD Systems To define a sequence click the line related to the sequence in the Type column then select a type Default Type value for sequences is Wait In order to prepare the entire protocol the type of each Uutput sequence must be selected As the module is started and the sequences follow one eae Output another the type of each sequence is displayed in the status p bar Each sequence is given a number by the module
2. NOTOCORD Systems DOC1 0s DOCIO 1 Applications The Digital Output Controller DOC10s software module allows control over various hardware devices Some of its common applications are the control of stimulators perfusion systems or aerosol delivery Its user friendly interface allows you to program the series of events as they should occur in the framework for your experimental protocol Warning CAUTION THIS SOFTWARE PRODUCT HAS NOT BEEN VALIDATED FOR USE ON HUMAN 2 Intended use DOC10s module makes it possible to define the protocol of an experiment by planning and by u r p SS p u setting the parameters for various sequences of Output signals likely to follow one another within the framework of an experiment and these signals can be e stimulation signals e control signals or output signals since they come out through the output channel During the stimulation sequences the software module controls the stimulation which materializes by periodic pulses of which the period and the width are adjustable During the output sequences the module sends out control commands which materialize by single short pulses of which the width is not adjustable During the wait sequences the module sends out wait commands which materialize by no signal emission Sequences likely to follow one another are limited to 99 November 28 2007 Edition 1 8 Stimulator control and stimulation signals Equipment co
3. 0 5 V maximum IOH 24 mA 0 5 V maximum IOH 12 mA 22 Q series resistor Specifications Output driver Output driver high voltage Output driver low voltage Stimulation Output ALS652 TTL 2 0 V minimum IOH 15 mA 2 4 V minimum IOH 3 mA 0 5 V maximum IOH 24 mA 0 4 V maximum IOH 12 mA Specifications Output driver Output driver high voltage Output driver low voltage Termination e DT9800 series November 28 2007 Edition ALS244 TTL 2 0 V minimum IOH 15 mA 2 4 V minimum IOH 3 mA 0 5 V maximum IOH 24 mA 0 4 V maximum IOH 12 mA 22 Q series resistor 7 8 NOTOCORD Systems Output Specitications Nomber of lines 8 Port B Termination 22 kQ resistor Output driver 74HCT244 TTL Output driver high voltage 2 4 V minimum IOH 1 mA Output driver low voltage 0 5 V maximum IOH 12 mA Diodes EMF Yes Stimulation Output Specifications Output driver high voltage 3 0 V minimum 1 mA Source 0 4 V maximum 2 mA Sink Output driver low voltage Bibliography DT3010 Series User s Manual Data Translation Inc Marlboro MA 4 ed March 2002 DT300 Series User s Manual Data Translation Inc Marlboro MA 7 ed March 2002 DT9800 Series User s Manual Data Translation Inc Marlboro MA 7 ed March 2002 November 28 2007 Edition 8 8
4. This i Sine number is the sequence order number 1 00 00 00 2 00 00 00 A comment can be added to each sequence To enter a ae comment click the line related to the sequence in the EEM Comment column then enter a short comment in the field that appears This comment will eventually be used to define event marker labels when the sequence is activated As the module is started and as the sequences follow one another the comment of each sequence is also displayed in the status bar Sequence description To delete a sequence simply click the Delete icon button i To start sequencing simply click the Start icon button O Important The DOC10s software module start does not depend on main NOTOCORD hem start Therefore it is recommended to first start signal acquisition and next start the stimulator Start times are displayed in the Start Time column For Start Time each sequence in each line there is a start time ee 15 16 00 A start time is defined for each sequence according to time 1516 05 when sequencing is started 15 16 06 To stop sequencing simply click the Stop icon button o To loop through the sequences simply click the Loop O icon button F November 28 2007 Edition 3 8 NOTOCORD Systems When sequencing is started progression is simple to visualize since the active sequence is highlighted in blue Besides the order number of the active sequence blinks in the status
5. bar DOC10s Stark Time Type Comment Duration s Channel Pernodimal Width ps 15 27 09 Stimulation Stimulation 1 15 27 20 Stimulation Stimulation 2 H 1 2 15 27 19 Walt Pause 3 4 15 27 25 Wi ait Pause O eo Stimulation Stimulation 1 00 00 05 Active sequences order numbers ea Inactive sequences order numbers Q Important 1 In order to use DOC10s to control a stimulator you must be using a NOTOCORD DT P6 ref hrd 1015 connection box only this connection box has the right Stim channel 2 When used with supported DataTranslation cards DOC10s can send out a stimulation signal on one channel only Remarks 1 To visualize outgoing signals simply proceed the same way you do to visualize any output signal from a module with an output 2 To visualize sequence changes with a marker simply connect DOC10s output with KBD 30a input November 28 2007 Edition 4 8 NOTOCORD Systems 3 Features Inputs and The DOC10s module has no input and only one output It outputs isan event type output and is discrete Output Period width T period for pulses in stimulation sequences goes from the T step and start of a pulse to the start of the next pulse duration of Range is from 20ms to 100s in steps of 1ms stimulation aD and wait TP width ange is from 0 1ms to 10ms in steps of lus sequences Minimum duration for a sequence is 1 s Maximum duration for a sequence is 10 hour
6. n the acquisition card used connections e on the DT301 and DT3010 series there are 8 input output channels among the 16 digital channels These channels are numbered 9 to 16 As soon as the DOC10s module is started all these channels become output channels only However it is possible to acquire the outgoing signals with these cards Stimulation signals come out on the clock channel Output signals come out on digital channels Caution Connecting DT301 cards to the NOTOCORD DT P6 ref hrd 1015 connection box requires only one cable but two cables are required to connect with DT3010 cards on DT9800 series there are 8 input channels and 8 output chanels To acquire output signals simply connect output and input channels Isolation Characteristics depend on the acquisition card used Ouipulsiage e DT300 series Outputs Port A Port B Port C Output driver FCT2574 TTL FCT2574 TTL ASIC TTL Output driver high voltage 2 4 V minimum 2 4 V minimum 2 4 V minimum IOH 15 mA IOH 15 mA IOH 4 mA Output driver low voltage 0 5 V maximum 0 5 V maximum 0 8 V maximum IOL 12mA IOL 12mA IOL 4mA November 28 2007 Edition 6 8 Stimulation Output NOTOCORD Systems Specifications Output driver Output driver high voltage Output driver low voltage Termination e DT3010 series Output ALS244 TTL 2 4 V minimum IOH 15 mA 2 4 V minimum IOH 3 mA
7. ntrol and output signals Defining a protocol NOTOCORD Systems To control a stimulator one must define the stimulation signal To define the T period click in the Period ms column in the required line and fill the right value in the field Values Period ms are expressed in milliseconds JILT To define the TD width when the stimulation signal logical level is high click in the Width us column in the _Wwidthips required line and fill the right value in the field Values are expressed in microseconds Th i To define the duration of the sequence click in the Duration s column in the required line and fill the right Durationts value in the field Values are expressed in seconds i Output sequences need no adjustments Equipment control simply responds to orders based on a short single signal transition However you can select specific output channels for these sequences aa Click in the Channel column in the required line and select a channel in the list Some experiments could require stimulator control and the control of an equipment at the same time Therefore it is necessary to define the sequences of each experiment For Action Potential measurements for example it may be recommended to program wait sequences between stimulation sequences after or while fibers are being washed Moreover the output sequences command equipment controls such as drug or cleaning pumps
8. s If a sequence ends in the midst of a pulse pulse level is set back to 0 Width and T durations in output sequences do not change This pulse duration of is only meant for equipment that responds to orders based output ona short single signal transition sequences D T duration of an output sequence lasts the time needed to start the next sequence and is approximately equal to the TD width of the pulse Pulse begins as soon as the sequence is started TD width is 50 ms Output signals can only come out on one channel at a time To get something close to coming out on multiple channels several output sequences should follow each other and they should be configured for signals to come out on different channels Precision e DT300 and DT3010 series Precision for period and width of stimulation signals depends on the precision of the clock on the acquisition card Precision for the width of the output signal depends on the operating system Equipment controled by these signals must not depend on a precise pulse width CPU load CPU load due to DOC1O0s software module is not significant November 28 2007 Edition 5 8 NOTOCORD Systems 4 Material Requirements In order to work DOC10s software module should run on a system with e DT9800 BNC Box and a connector provided by NOTOCORD Systems e DT301 or DT3010 card and the NOTOCORD DT P6 ref hrd 1015 connection box Cables and Cables and connections depend o

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