SPEKOL 1500 UV VIS Spectrophotometer
Contents
1. 13 6 2 Replacing fUSes a JJ ots as a Ces det et mm mmmmpm tds 13 6 3 Replacing Halogen lamp ui kk emen 14 6 4 Replacing Deuterium lamp esee emen 15 7 ACCGSSODy Ez r k se E HEEE ei HESTE DE cavan A EE 17 7 1 Manual 4 cell changer ener nnns 17 7 2 Adjustable cell holder eret etienne tes 17 7 3 Round cell holder eL 18 7 4 Extension of sample compartment kk kek 18 7 5 Flow through Unit e 2 a HHH HHHH EH HH remz 19 7 6 Thermostatted cell holder iind s 19 7 7 Thermostatted 3 cell manual changer sse 19 7 8 Printer nite toan imei uu n Eu 20 8 Display and Buttons of SPEKOL 1500 21 9 Software nen E 23 9 1 eier 23 9 2 Quantitative measurement cineres tete dereud 24 9 3 Wavelength scan E E ETE AT E T 26 9 4 Kinetic Analysis senesine ea ee deel eee E Ea 27 9 5 Multi Wavelength Measurement kk kk kk kek ka 28 9 6 DNA Protein measurement llllliii kk kk kek kk kk E kk kK AKA KA 28 9 7 Systeri Set p EEN 29 User s manual SPEKOL 1500 Edition 07 2008 1 Analytik Jena AG Contents 9 8 System positioning cce tenete te tei eniin nee trito dei exea 30 9 9 Lights and A D Comversion ene eene 30 9 10 euenit N e rr _rl lc erra 31 10 DIS DOSal ie SIRR WE e 33 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG2. Mode Strike F2 Follow the command please select mode by striking Vi or Abs T or Conc can be selected Please confirm your input striking Ent K Factor Strike F3 After Please input Factor was displayed a value between 0 000 and 9999 can be entered Standard Strike F4 Follow the command Please input Conc of standard input a value between 0 000 and 9999 and confirm Ent User s manual SPEKOL 1500 Edition 07 2008 23 Analytik Jena AG Software function Quantitative measurement 9 1 1 Measurement in photometric mode CP Note Pay attention that the second step of the manual cell changer is only for reading O T There are two steps between Position 1 and 2 of the manual cell changer Choose cells with the same path length for all blanks standards and samples of the analytical method you are using For measurements below 325 nm use quartz cells Glass or plastic cells are only usable above 325 nm The liquid level must be at least 20 mm in a standard square cell 1 Set the wavelength by striking Set WL Follow the command Please input wi input the value and confirm Ent The system will move to the entered wavelength 2 Strike F2 to select the mode 100 T BASELINE 3 When measuring T or Abs put in the reference solution and tap to adjust blank 4 Putin the sample The value is read out instantly To determine a concentrat
3. For setting a standard curve please strike F 1 to select a unit and confirm Ent 4 Strike F2 to set the standard calibration curve Tap F1 to select the fit method and confirm Ent Strike F3 to enter the standard s settings Follow the message Edit the number and confirm Ent Now Input standard Conc and confirm Ent Input further values accordingly Tap Esc to end the setting 5 Performing the measurement After having left the settings by striking Esc the message Blank First will appear Put in the reference substance and strike 100 T BASELIN accordingly After measurement has been completed the corresponding equation will be displayed Other fit methods can be chosen and the system will change the parameters accordingly 6 Strike F4 to view the curve Now follow the message Place Standard 1 and proceed To save the standard curve strike Save Follow the message Please input file name and confirm Ent The file will be saved in STD format 8 After the standard curve has been recorded successfully strike Esc In order to MEASURE determine a sample s concentration strike After the message START Blank first is displayed put in the blank of sample and strike Ent Now follow MEASURE the message Place sample and strike The result is read out START instantly U
4. With the cursor keys Ni or ft you may select Yes or No If No was selected the operation is cancelled If Yes all the files stored will be deleted User s manual SPEKOL 1500 Edition 07 2008 29 Analytik Jena AG Software function System positioning 9 8 System positioning Please enter the mode by either striking 8 ruv or using the cursor keys N or ff and confirm Ent In order to select the operation mode either press a suitable number key to enter the interface or use the cursor keys N or N Now strike Ent to enter the interface The following menu items are accessible 1 Wavelength Calibration 2 Search the first filter 3 Change the filter 4 Set slit width 5 System calibration Wavelength calibration Find the D2 lamp s particular spectrum line at 656 1nmn to adjust precision of the instrument s wavelength positioning Search The First Filter Change The Filter This functions are not valid for the user Set Slit Width This function is not valid for SPEKOL 1500 System Calibration Restores default settings measures dark current and position of the light At last the position of D2 lamp s particular spectrum line at 656 1nm checked by the system 9 9 Lights and A D Conversion Please enter the mode by either striking 9 wxvz or using the cursor keys Vi or l land confirm Ent In order to select the operation mode either press a suitable number key to enter the i
5. Introduction 1 Introduction The SPEKOL 1500 is a single beam spectrophotometer The instrument is compact easy to use and rugged The large sample compartment accepts various accessories and cell from 10mm to 100mm path length The built in RS 232 serial interface enables the instrument to be connected to a printer The SPEKOL 1500 is ideal to use in a clinical laboratory biochemical laboratory petrol chemical laboratory environmental protection and various fields of quality control This user s manual provides the description of the SPEKOL 1500 a list of main specifications and available accessories After carefully unpacking the content check the materials against the packing list to ensure that you have received everything in good condition If some parts are missing damaged or have any other defects please contact your dealer or the sales representative immediately 1 4 Warning and safety labels on SPEKOL 1500 The following warning and safety labels are located on the SPEKOL 1500 A Caution Hazardous area Do not remove this cover under any circumstances Trained service people only No serviceable components inside Select the right voltage Disconnect the power cable before opening the device cover or replacing fuses User s manual SPEKOL 1500 Edition 07 2008 3 Analytik Jena AG Introduction 1 2 Notes on the User s Manual In this manual the following symbols are used to refer
6. To enter the peak search mode please strike F3 The function shortcut Threshold F1 is active Striking F1 leads to Please input peak height Confirm your input and press the key N or to search a peak Use the cursor keys or gt to display ordinate values at selected wavelengths above threshold Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Software function Kinetic Analysis 9 4 Kinetic Analysis In this mode time dependent changes of absorption or transmittance can be recorded It is possible to do a correlation analysis To enter the mode please strike either 4 cui or use the cursor keys Vi or fi and Ent There are the following function shortcuts Setup F1 Mode F2 Process F3 and Search F4 Setup Please strike F1 Enter the total measuring time s and confirm Ent Input time interval using the cursor keys N or f and confirm Ent Mode Striking F2 leads to the message please select mode Select between Absorption or Transmission using the cursor keys W or f and confirm Ent Process Calculates a U value Strike F3 Enter a Begin Time an End time and a Factor and confirm Ent respectively The result is read out instantly Search Strike F4 to enter the search mode Tap 4 or 5j to move along the abscissa from data point to data point 9 4 1 Measurement in mode Kinetic
7. for cells with an aperture height of 8 5 mm User s manual SPEKOL 1500 Edition 07 2008 17 Analytik Jena AG Accessory Screw 1 Fig 9 SPEKOL 1500 adjustable cell holder 1 Insert the adjustable cell holder in the sample compartment with screw 1 to the back site 2 Use the screw 1 to adjust the cell horizontal to the light beam and screw 2 to adjust the cell vertical to the light beam The cell has the optimal position if the highest transmittance is reached Together with the extension of sample compartment 8 4 and an external pump also flow cells can be used with this cell holder 7 3 Round cell holder This special cell holder can be used for round cell of standard test kits Cells with a diameter up to 16 mm and a height up to 110 mm can be used 7 4 Extension of sample compartment This extension is an attachment for tube openings that allows the use of thermostatted cell holders It can easily be adapted to the SPEKOL 1500 sample compartment Fig 10 SPEKOL 1500 extension of sample compartment 18 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Accessory 7 5 Flow through unit The flow through unit requires the adjustable cell holder the extension of sample compartment and a pump system 1 2 3 6 7 Insert the adjustable cell holder into the sample compartment Mount the extension on the border of the sample compartment Slide the obliquely cut end of P
8. given on the labels and the information on the use storage and disposal of reagents reaction cells and the notes on the package inserts of test kits Intended use The SPEKOL 1500 including original accessories may only be used for the applications described in this manual The manufacturer cannot assume any liability for any other application including that of individual modules or single parts This also applies to all service or repair work that is not carried out by authorized service personnel All warranty claims shall be forfeited Local safety regulations Observe the local safety regulations relevant to the operation of the device e g labor safety regulations regulations for the prevention of and protection against accidents References to possible risks given in this manual do not replace the relevant labor safety regulations that are to be followed Personnel The SPEKOL 1500 may only be operated by appropriately qualified and trained personnel For that the knowledge of the information provided by this manual is indispensable and taken for granted Emergency stop In the case of emergency disconnect the SPEKOL 1500 and its components from line power by removing the power plug from the power outlet Caution This procedure involves the risk of data loss and damage to the operating system Electric shocks The SPEKOL 1500 is electrically powered At several parts inside the system extremely ha
9. you to warnings and notes Warning Warning messages alert you to a specific procedure or practice which if not followed correctly could cause personal injury Caution Caution messages refer you to procedures which if not observed could result in damage to the equipment Caution Dangerous electric voltage Caution Emission of UV radiation D gt gt gt Disconnect power cable Note This note must be followed to obtain correct measurement results q The following symbols are used gt Cross reference Refers to another section illustration of the manual For easier navigation within the manual the manual uses the following system e Chapters and illustrations are numbered consecutively e Every illustration has its own caption e Steps of operation are numbered e Cross references are marked by an arrow e g Notes on the User s Manual p 3 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Safety notes 2 Safety notes gt p e For your personal safety and for a trouble free operation carefully read this chapter before starting the SPEKOL 1500 Observe all safety notes given in this manual and all messages and notes displayed by the control software on the screen In addition observe the safety notes for system components of other manufacturers e g PC printer supplied together with the device In particular you should also observe the safety notes
10. 2008 13 Analytik Jena AG Maintenance Replacing Halogen lamp 6 3 Replacing Halogen lamp Electric shock Before changing the halogen lamp make sure to switch the device off with the power switch d Caution Risk of burns Allow the halogen lamp to cool down to avoid the risk of burns AN Caution Eye injury Never look into the lighting lamp as the radiation may cause eye injury A Caution Do not touch the glass bulb of the lamp to avoid any contamination affecting its performance 7 Turn the power off and unplug the instrument 8 Put all samples and not fixed elements out of the sample compartment 9 Place the SPEKOL 1500 carefully on a soft underlay on the top side of the device so that you can access the underside 11 Fig 4 Lamp house cover on the under side of SPEKOL 10 Remove the two screws 11 and the lamp house cover 12 on the underside of the instrument Fig 5 Lamp compartment of SPEKOL opened 14 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Maintenance Replacing Deuterium lamp 11 Unscrew the holder of the halogen lamp 14 and take the holder with the halogen lamp out of the device Fig 6 SPEKOL 1500 holder of the halogen lamp 12 Remove the lamp 16 from lamp holder 13 Replace the new lamp carefully in the holder A Caution Do not handle Halogen lamp with bare fingers Use a piece of tissue or cloth when handling the lamp The oil from your f
11. 7 8 Printer 1 2 3 To install the printer to the SPEKOL 1500 switch the device off plug the electrical connections into the RS 232 at the right side Fig 2 4 p 11 and the printer power connection at the back side of the device Fig 3 6 p 12 Restart the instrument 20 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Display and Buttons of SPEKOL 1500 8 Display and Buttons of SPEKOL 1500 Fig 12 Display and buttons of SPEKOL 1500 Display The digital readout displays the data of transmittance absorbance and concentration and scanned spectra H H Id Setup X Y axis move up down left right or choose the item When inputting a letter a switch between capital and small letters can be b made using f or V ESC Exit return to the last operation or cancel the operation Print Print Cell Not valid for SPEKOL 1500 Set WL Set a wavelength 100 T Adjust 100 T or adjust blank or calibrate baseline BASELINE MEASURE Start measurement START CE Delete delete input cancel edit delete measured data delete files Ent Enter Save Save file Open Open file F1 F2 F3 F4 Keyboard shortcuts 0 Del Character key 24sec 9wxvz Li I User s manual SPEKOL 1500 Edition 07 2008 21 Analytik Jena AG Display and Buttons of SPEKOL 1500 22 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG So
12. Analysis 1 Strike Set WL follow the message Please input wl and confirm Ent The system will now move to the selected wavelength and adjust blank automatically 2 Enterthe setup striking F1 enter the total measuring time s and confirm Ent 3 Input time interval using the cursor keys N or j and confirm Ent 4 Striking the cursor keys f or H leads to the message Min Y Enter a value and confirm Ent and proceed analogously with Max Y 5 Select absorption or transmission striking F2 100 T 6 Putin the reference and strike _ __ to adjust blank BASELINE MEASURE 7 Putin the sample strike The graph is shown instantaneously START 8 To save data tap Save Follow the message Please input File Name and confirm Ent The file will be saved in kin format 9 To calculate I U values please strike F3 and input Begin Time End Time and a Factor and confirm Ent respectively The result is read out instantly and may be printed p Note The maximum of total time is 30000s and the maximum number of data points should not exeed 4000 User s manual SPEKOL 1500 Edition 07 2008 27 Analytik Jena AG Software function Multi Wavelength Measurement 9 5 Multi Wavelength Measurement Multi Wavelength measurement is suited for checking the sample s absorbance or transmittance at several
13. Before exchanging a lamp please switch of the SPEKOL 1500 unplug the divice and wait until the lamps cooled down Accumulation of heat Heat accumulation might result in overheating and faults on the device Make sure not to cover the ventilation slots of the SPEKOL 1500 Before you switch on the SPEKOL 1500 remove its dust cover Handling liquids Take care to ensure that no liquids can enter the SPEKOL 1500 to avoid any damage to the device Risk of corrosion Do not set up the device in the vicinity of aggressive vapors e g strongly corrosive acid or caustic vapors The vapors might corrode the connections mechanical and optical components of the device Avoid exposing the sample compartment to strongly corrosive substances For the analysis of such samples use vapor tight stoppered cells After the measurement do not leave the samples unnecessarily long in the sample compartment of the device Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Technical data 3 Technical data Model SPEKOL 1500 Optical System Littrow type single beam grating system 1200 Line mm Spectral Slit width 2nm Wavelength Range 190 to 1100 nm Wavelength Accuracy 2nm Repeatability 1nm Stray Radiant Energy 0 3 T at 220 nm and 340nm Photometric Range 0 T to 125 0 T 0 1 A to 3 A 0 C to 9999 C Stability 0 002 A hr at 500 nm after 1 hour warm up Photometric Accura
14. SELINE MEASURE 4 Place your sample and strike As soon as measurement is START completed the result will be read out reference and strike 5 To save your data please strike Save Follow the message Please input File Name and confirm Ent The file will be saved in dna format 9 7 System setup Please enter the mode by either striking 7 pars or using the cursor keys or and confirm Ent In order to select the operation mode either press a suitable number key to enter the interface or use the cursor keys N or Now strike Ent to enter the interface The following menu items are accessible 1 Restore default settings 2 Beeper on off 3 Select language 4 Printer setup 5 Format file system Restore default settings This operation would reset the instrument to its original settings Files are not formatted Beeper on off If the beeper is activated the icon of a loudspeaker is displayed At the default state the beeper is on Select language The message Select Language appears With the cursor keys 4 or f you can select Chinese English or German The message Printer is displayed With the cursor keys N or ff you can select from the following printers SPRT SP _T HP PCL 1 color cartridge HP PCL black mode or SPRT SP POS76II Format file system The display shows Delete entire files are you sure
15. TFE sipping hose over the hose stern on the cell body marked with an arrow Put the silicon sleeve of the sipping hose over the stern in order to make the connection leak proof Slide the other hose end through one of the tube opening Slide the pump hose through the second tube opening Connect the second cell stern with the pump system Insert the flow through cell in the cell holder and adjust the cell section Adjustable cell holder on p 17 Start the flow through operation Stop the flow through operation Read the measurement values The carry over from sample to sample can be kept low if an ample portion of the sample is used for rinsing the cell before a measurement 7 6 Thermostatted cell holder The water thermostatted cell holder can be used for temperature dependent measurement of samples It is possible to connect the thermostatted cell holder to an external thermostat This accessory is available for cells with 10mm or with 50 mm pathlength 7 7 Thermostatted 3 cell manual changer The 3 cell water jacketed manual changer is available for cell with 10mm pathlength Use the cell selector arm Fig 1 p 11 of the manual cell changer to set the position of the manual cell changer Position 4 Position 3 Position 2 Position 1 Sample 2 Sample 1 0 T Reference Fig 11 Position of 3 cell changer User s manual SPEKOL 1500 Analytik Jena AG Edition 07 2008 19 Accessory
16. analytikjena SPEKOL 1500 UV VIS Spectrophotometer lytik J AG analytikjena Kundendienst Konrad Zuse Str 1 07745 Jena Phone Hotline 49 3641 77 7407 Fax 49 3641 77 7449 e mail service analytik jena de General information about Analytik Jena AG on the internet http www analytik jena de Copyrights and Trademarks Microsoft Windows 2000 XP Vista MS Excel are registered trademarks of Microsoft Corp Edition July 2008 Technical documentation made by Analytik Jena AG This documentation describes the state of this product at the time of publishing It need not necessarily agree with future versions of the product Subject to change Analytik Jena AG dez O Copyright 2008 Analytik Jena AG milia Contents Contents 1 Introd UC HOT ere eee end rica Ce Lo Peas rO rere 3 1 1 Warning and safety labels on SPEKOL 1500 sss 3 1 2 Notes on the User s Manual 4 2 Safety notes cusseeedcacedsdeedtcaedaanodtetecsrnasundaeddducuesadudvededaueataeten 5 3 Technical data sue odere vusvescuaadivenctecncauezcetustes 7 4 Unpacking instruction ioi ns ciya lan ee s ka da eee sue nna 9 4 1 Installation and transport conditions kk 9 4 2 Unpacking instruction ciiin rent innere idet He kek KEV Alka k k 10 5 Design ofthe SPEKOI Dee 11 6 Mailnte ran C s ss cien et ecerecdutrtsnesecanstecacctbaseeranevedsusedarreetass 13 6 1 Cleaning E
17. ce is over dispose of the device with its electronic components as electronic scrap observing the relevant regulations User s manual SPEKOL 1500 Edition 07 2008 33 Analytik Jena AG
18. cy 0 01A Power Requirement 115 V 230 V adjustable Frequency 50 to 60Hz Line fuses 2x T 4 AH Temperature working range 15 C to 35 C Humidity working range 85 Size W x D x H 465 mm 18 3 x 365 mm 14 3 x 175 mm 6 9 Weight 11 5 kg User s manual SPEKOL 1500 Analytik Jena AG Edition 07 2008 7 Technical data Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Unpacking instruction Installation and transport conditions 4 Unpacking instruction 4 1 Installation and transport conditions Installation conditions Considering the dimensions of the SPEKOL 4500 and a clearance of approximately 100 mm to the sides and the front the device needs a bench space of at least 665 x 565 mm Caution Do not cover the ventilation slots of the device Do not place any objects on the device Do not operate the device in explosion risk rooms Keep aggressive vapors e g strongly corrosive acid or caustic vapors away from the vicinity of the device The installation site of the SPEKOL 1500 must meet the following requirements e The place should be free of draft dust corrosive vapors as well as vibrations e Donot set up the SPEKOL 1500 near electromagnetic fields e g of motors e Avoid dripping or splashing water near the instrument e Do not expose the device to direct sunlight or the radiation of heaters e Temperatur
19. e range 15 35 C e Humidity up to 85 at 30 C Conditions for storage and transport For transport and storage the following ambient conditions must be complied with e Temperature range in transport 40 70 C e Relative humidity in transport up to 95 User s manual SPEKOL 1500 Edition 07 2008 9 Analytik Jena AG Unpacking instruction Unpacking instruction 4 2 Unpacking instruction A amp Observe correct position of device Move the SPEKOL 1500 only in upright position observe label on transport case Do not turn the device upside down Electric shock Before connecting the SPEKOL 1500 and the PC to line power make sure the available line voltage agrees with the operating voltage specified on the type labels Operation of the devices on line voltages other than the specified operating voltage may result in their destruction 1 Unpack the contents carefully and check the materials 2 Remove any obstructions or materials that could hinder the flow of air under and around the instrument vibration Select the right voltage 4 Plug the power cord into an outlet Make sure that the first position of the cell changer is set Remove anything in the sample compartment interfering with the light beam 6 Turnon the instrument A self test starts automatically Caution Please allow the instrument to warm up for 20 minutes before taking any readings Your SPEKOL 1500 i
20. ew the 2 screws of the deuterium lamp 13 disconnect the electronic plug connection 15 and take the deuterium lamp out of the device amp Do not handle Deuterium lamp with bare fingers Use a piece of tissue or cloth when handling the lamp The oil from your fingers can cause the lamp to burn out prematurely 6 Replace the new deuterium lamp and fix it with the screw 7 Close the cover of the lamp house using the 2 screws 8 Replace the device with the top side on top 16 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Accessory 7 Accessory For the installation of accessory use the two screws on the bottom of the sample compartment 7 1 Manual 4 cell changer The standard configuration is a 4 cell changer for 1 cm cells As an option there are also 4 cell changers for cells with 50 and 100 mm path length available Fig 7 Manual 4 cell changer Use the cell selector arm Fig 1 p 11 of the manual cell changer to set the position of the manual cell changer Sample 3 Sample 2 Sample 1 0 T Reference Position 5 Position 4 Position 3 Position 2 Position 1 Fig 8 Positiond of the 4 cell changer 7 2 Adjustable cell holder Micro cells and ultra micro cells have the standard outside width of 12 5 mm but a reduced inside width or filling aperture of 1 to 4 mm This requires accurate alignment of the cell in the light beam The adjustable cell holder can be used
21. ftware function Photometric mode 9 9 1 Software function After the instrument was switched on a self testing procedure will be performed When this has been finished the beeper will ring three times and the system will enter the main interface 1 Photometric mode 2 Quantitative measurement 3 Wavelength scan 4 Kinetics analysis 5 Multi Wavelength measurement 6 DNA Protein measurement 7 System setup 8 System positioning 9 Lights and A D conversion 10 Connect to PC In order to select the operation mode either press a suitable number key to enter the interface or use the cursor keys or j Now press Ent to enter the interface When the instrument is restarted the system will remember the last parameters automatically Photometric mode In this mode basic absorbance and transmittance measurements as well as concentration measurements can be performed To enter the mode please strike either 1 4 or use the cursor keys W or T and Ent There are four function shortcuts Unit F1 Mode F2 K Factor F3 Standard F4 Unit Strike F1 to enter the setup After appearance of the message Please select unit strike N or i The following units can be selected mg L g L ng g ug g IU mM L MIL ug ml mg ml other In case other was selected please confirm by striking Ent Follow the message Please input self defined unit and confirm by striking Ent
22. ingers can cause the lamp to burn out prematurely 14 Replace and fix the holder with the new halogen lamp in the device 15 Close the cover of the lamp house using the 2 screws 16 Replace the device with the top side on top 6 4 Replacing Deuterium lamp N Risk of electric shock Before changing the deuterium lamp make sure to switch the device off with the power Switch Caution Risk of burns Allow the deuterium lamp to cool down to avoid the risk of burns Emitted UV radiation Never look directly or indirectly e g via a mirror into the radiation emitted by the lamp to avoid the risk of getting conjunctivitis Dirt on beam exit window gt Do not touch the glass bulb of the new deuterium lamp Particularly avoid contamination on the quartz glass beam exit window If you touched the glass bulb with your fingers wipe the fingerprints off with a clean lintfree cloth and pure alcohol The performance of the lamp will deteriorate by contamination 1 Turn off the power and unplug the instrument 2 Putall samples and not fixed elements out of the sample compartment User s manual SPEKOL 1500 Edition 07 2008 15 Analytik Jena AG Maintenance Replacing Deuterium lamp 3 Place the SPEKOL 1500 carefully on a soft underlay on the top side of the device so that you can access the underside 4 Remove the two screws 11 and the lamp house cover 12 on the underside of the instrument 5 Unscr
23. ion strike F1 and select a unit Perform measurement 100 T BASELINE 6 Putin the sample If the sample has a known K factor please strike F3 and input your K factor The system will measure the absorbance and the concentration is read out directly of blank striking the key 7 Incase the K factor of the sample is unknown you have to use a standard first Please strike F4 input the standard concentration and confirm Ent The system will measure the absorbance and calculate the K factor K Conc Abs Now the sample can be measured The concentration will be read out directly 9 2 Quantitative measurement To enter the mode please strike either 2 aec or use the cursor keys N or 1 and Ent In this mode there are two operating states Quantitative test and Standard calibration curve The first operating state when entering the mode is Quantitative test Quantitative measurements can be performed directly with known coefficients Determination of concentration may also be calculated according to a standard calibration curve see 10 2 1 Two function shortcuts are accessible Unit F1 Fit Curve F2 Unit Enter the setup tapping F1 After appearance of the message Please select unt strike or f The following units can be selected mg L g L ng g ug g 96 IU mM L MIL ug ml mg ml other In case other was selected please confirm by striking Ent Follow the mes
24. ited for quantitative analyses and purity testing of DNA or proteins according to the DNA and protein s special absorbance in the UV range Please enter the mode by either striking 6 mno or use of the cursor keys Vi or l and confirm Ent Two function shortcuts are active Mode F1 Unit F2 Mode Strike F1 The message Measurement appears Using the cursor keys W or you can now select Absorbance difference 1 260 and 280 nm or Absorbance difference 2 260 and 320 nm Please confirm your choice Ent After the message With Reference you can choose wether to use a reference or not The reference wavelength is 320nm Unit Strike F2 Please follow the message Please select unit and strike N or ff The following units are programmed mg L g L ng g ug g IU mM L M L pg ml mg ml other If other was selected please confirm striking Ent Follow the message Please input self defined unit and confirm striking Ent Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Software function System setup 9 6 1 Measurement in DNA Protein measurement mode 1 Strike F1 Choose measuring mode Absorbance difference 1 or Absorbance difference 2 and whether to measure a reference sample 2 Strike F2 to select a measuring unit 3 f you agreed to carry out your analysis with reference measurement put in the 100 T BA
25. lues at selected wavelengths 9 3 1 Measurement in mode Wavelength Scan 1 To set the scan parameters please strike F1 Enter a maximum wavelength when the message Scan From appears and confirm your entry Ent 2 Enter the minimum wavelength according to the message Scan To and confirm Ent 3 Choose Scan Step using the cursor keys W or l and confirm Ent Step width can be selected from 1 to 5 0 nm Please note that the maximum number of data points per scan is 1001 4 Select a Scan Speed using the cursor keys Hi or l and confirm Ent The speeds Fast Normal and Slow are available 5 Strike the cursor keys f or N to set the Y axis Enter ordinate values for min Y and confirm Ent Proceed analogously with max Y 6 Strike F2 and select a scan mode Abs or T using the cursor keys WV or f l and confirm Ent 100 T NEN 7 Putin the reference and strike to measure a calibration baseline BASELINE l MEASURE RD l 8 Putin the sample and strike The spectrum is displayed instantly START 9 After a finished scan the wavelength is set to the starting point 10 To save your scans strike Save Follow the message Please input File Name and confirm Ent The file will be saved in wav format 11
26. nterface or use the cursor keys VI or f Now strike Ent to enter the interface The following menu items are accessible 1 Switch On Off D2 lamp 2 Switch On Off W lamp 3 Change D2 W switch point 4 Set amplifier 5 Set W lamp power Switch On Off D2 or W Lamp If the D2 or W lamp was already switched on this procedure would switch it off Operation state of the lamp is shown in the upper right corner of the display If a lamp is switched on on is displayed Change D2 W Switch Point Follow the message Input the D2 W switch point and confirm Ent The switch point may be set from 300 to 360nm Set Amplifier Set W Lamp Power This function is not valid for the user Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Software function Connect to PC 9 10 Connect to PC On operation the display shows Connecting to computer If this procedure was successful the message PC Connected is displayed To quit PC connection please strike Esc User s manual SPEKOL 1500 Edition 07 2008 31 Analytik Jena AG Software function Connect to PC 32 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Disposal 10 Disposal The owner operator of the SPEKOL 1500 must ensure to dispose of the waste material samples cropping up in the analyses in accordance with the relevant legal and local regulations When the service life of the devi
27. only authorized service representatives to perform A procedures requiring removal of the instrument s cover and replacement of electrical components To protect both of yourself and your instrument be sure to contact the authorized service representative to perform any service procedure when you do not feel comfortable performing 6 1 Cleaning If sample substance was spilled in the sample compartment or accessory instantly wipe it up using tissue paper For cleaning the device cover from dust or spillings please use a soft cloth and a commercial neutral cleanser Do not expose SPEKOL 1500 to corrosive atmosphere 6 2 Replacing fuses If the line fuses are defective you can change them yourself Risk of electric shock Before changing the fuses make sure to switch the SPEKOL 1500 off with the power switch and disconnect the power cable from the power input connector of the device 1 Remove the power cable from the SPEKOL 1500 2 Open the bayonet connections of the fuse holders gt Fig 3 p 12 by turning to the left using a screwdriver The fuseholder jumps up a little 3 Replace the defective line fuses Make sure to use only the following fuse types e T4AH 4 Re insert fuse holder and lock the bayonet connection 5 Reconnect the power cable to the power input connector of the SPEKOL 1500 and the power outlet 6 Switch the SPEKOL 1500 on again User s manual SPEKOL 1500 Edition 07
28. p to 100 values can be stored in the table Should the data be saved please strike Save The file will be saved in QUA format User s manual SPEKOL 1500 Edition 07 2008 25 Analytik Jena AG Software function Wavelength scan 9 3 Wavelength scan In this mode absorbance or transmittance spectra can be recorded It is possible to do a correlation analysis To enter the mode please strike either 3 per or use the cursor keys Vi or f and Ent There are the following function shortcuts Setup F1 Mode F2 Search F3 Setup Strike F1 to enter the setup Please note that the system scans from the maximum wavelength to the minimum wavelength Enter a maximum wavelength when the message Scan From appears and confirm your entry Now enter the minimum wavelength according to the message Scan To and confirm Ent Next choose Scan Step using the cursor keys Ni or 1 and confirm Ent Input the Scan Speed analogously Mode Striking F2 leads to the message Please select mode With di or I display of Absorption Abs or Transmission T can be selected Search After the scan has been completed please strike F3 to enter the search mode The function shortcut Threshold F1 is active Striking F1 leads to Please input peak height Confirm your input and press the key NJ or f to search a peak Use the cursor keys or 5j to display ordinate va
29. s now ready for operation 10 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Design of the SPEKOL 1500 5 Design of the SPEKOL 1500 1 2 3 1 Display and Button display of data s and control buttons for use of the internal software function 2 Sample compartment to insert accessories and sample cells 3 Cell selector arm of the Cell changer to change the position of the manual cell changer Fig 1 SPEKOL 1500 front side Cr Note Pay attention that the cover of the sample compartment must be closed during the measurements Printer 4 Printer connection 5 RS 232 Interface Fig 2 SPEKOL 1500 right side User s manual SPEKOL 1500 Edition 07 2008 11 Analytik Jena AG Design of the SPEKOL 1500 6 Power cable socket 7 Printer power connection power cable socket from the printer 8 Fuse holder 9 Voltage selection to select the voltage between 220 and 110 V 10 Power switch Fig 3 SPEKOL 1500 back side 12 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Maintenance Cleaning 6 Maintenance This SPEKOL 1500 spectrophotometer has been designed to be durable and reliable Therefore the routine maintenance of the instrument is minimal However you will need to clean spillings or to replace the lamp or you may want check the wavelength calibration and photometric linearity to verify the instrument s performance The manufacturer recommends
30. sage Please input self defined unit and confirm by striking Ent Fit curve Strike F2 to enter the standard curve operating state begin the next setup Four function shortcuts are available Method F1 Params F2 Standard F3 Show curve F4 Edition 07 2008 User s manual SPEKOL 1500 Analytik Jena AG Software function Quantitative measurement Method Tap F1 to enter the setup for Fit method Using the cursor keys duor f one can choose between these methods Linear fit through zero Linear fit Square fit Cubic fit Params To modify coefficients strike F2 With a selected method Linear fit through zero the message Input K1 would appear Input the value and confirm Ent Subsequently Input r and confirm Ent Having selected Cubic fit the factors KO K1 K2 K3 need to be entered For the other methods available please proceed analogously Standard Strike F3 to enter the standard setting Follow the message Edit the number and confirm Ent Now Input standard Conc and confirm Ent Input further values accordingly Tap Esc to end the setting Show Curve To display the standard curve after measurement has been completed please strike F4 1 Measurement in quantitative measurement mode 2 Strike Set WL The message Correction method appears Choose between the methods Single WL 3 Points Isoabsorbance using the cursor keys W or and confirm Ent
31. wavelengths at one time Please enter the mode by either striking 5 k or use of the cursor keys Hi or l and confirm Ent There are two function shortcuts available WL Setup F1 Mode F2 WL Setup Strike F1 to enter the setup Follow the message Edit the number and confirm Ent Now Please input wl and confirm Ent Please input further values accordingly Strike Esc to end the setting Mode Strike F2 Now please select mode using the cursor keys y or lT to select Abs or T Confirm striking Ent 9 5 1 Measurement in mode Multi Wavelength Measurement 1 To set parameters please strike F1 Follow the message Edit the number and confirm Ent Now Please input wl and confirm Ent Please input further values accordingly Strike Esc to end the setting 2 Select the display mode F2 100 T 3 Putin your reference and strike BASELINE MEASURE l 4 Place the sample and strike to measure Note During START measurement process the system will follow the sequence wavelengths were set When a measurement process is completed the wavelength is reset to the first wavelength 5 To save your data please strike Save Follow the message Please input File Name and confirm Ent The file will be saved in mul format 9 6 DNA Protein measurement This mode is su
32. zardous electrical voltages are accessible To ensure Protection Class protective earth connection of the device connect the power plug of the device only to a power outlet with protective earth conductor The protective effect must not be undone by the use of an extension cable without protective earth conductor Before opening the device or removing any device covers disconnect the power cable User s manual SPEKOL 1500 Edition 07 2008 5 Analytik Jena AG Safety notes gt GGG D Before connecting the SPEKOL 1500 to the power outlet make sure the line voltage supplied agrees with the operating voltage specified on the rating plate at the rear panel of the device Operation of the device with any other operating voltage than that specified may result in the destruction of the device Only use fuses of the specified type Do not operate the device in explosion risk rooms UV radiation Never look directly or indirectly e g via a mirror into the radiation emitted by the lamp to avoid the risk of getting conjunctivitis Therefore switch off the lamp for lamp replacement Lamp damage Do not grasp functioning halogen and deuterium lamps on their glass bulbs Contamination on the bulb affects the radiation properties of the lamps Especially protect the beam exit window of the deuterium lamp Risk of burns The lamp housing will heat up when lamps are operating Contact with hot surfaces may cause burns
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