User Manual Lx POL - Labo America, Inc.
Contents
1. Slide glass Cover glass Fig 28 i Rotate the coarse adjustment knob 2 in anticlockwise direction to fully lower the stage 2 Press the spring loaded clip lever 3 by pushing lever handle 1 place the specimen by sliding the specimen glass plate s on the stage 3 After positioning your specimen slides 1 max release the lever handle 4 Rotate the stage 360 to achieve best viewing position The degree of position can be noted Cover glass This is the glass plate placed on the specimen For optimum optical performance the cover glass thickness which is the distance from its surface to the specimen surface should be 0 17 mm Slide glass This glass plate should ideally have a length of 76 mm width of 26 mm 1 mm and thickness between 0 9 and 1 4 mm Vernier Scales These scales allow for easy identification of the specimen s position coordinates making it easy to return to a particular region of interest after scanning the slide Figure 29 21 Adjusting the Focus Focusing Procedure Figure 30 lt 3 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We Wb recommend starting with 10X O 2 While observing the specimen through the eyepieces slowly rotate 1 the coarse adjustment knob 1 counterclockwise to lower the 2 s2. Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 991 9862 CA 94538 sales laboamerica com LABOMED and Lx POL are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2009 Labo America Inc 9126000 990A 02 2009 Printed in U S A
3. amp the Analyzer It allows to introduce compensator amp retardation plates between the cross polarizer which can enhance optical path differences in the specimen details Lx POL objectives are free from stress amp both type of strain i e glass characteristics during various stages of the assembly like cementing or mounted in close proximity with tightly fitted frames Lx POL objectives are marked P on objectives barrel only after passing the strict testing Lx POL comes with standard 4x 10x 40x objectives that solve the purpose of viewing specimen in conoscopic amp orthoscopes modules These objectives are anti refraction coated Some other objectives like 2 5x 20x amp 100x are also available 13 Eye Pieces Fig 12 Bertrand Lens Lx POL comes with a pair of Eye Pieces One of the eyepieces has a crosshair reticule to mark the center of the field of view and the second for normal viewing Orientation of the Eye Piece with respect to the Polarizer and Analyzer is ensured by the eye tube lower that slides into the observation Bino Body tube These eye pieces also have a focusing mechanism for diopter correction amp foldable eye guards to prevent ambient light from entering into your line of vision Lx POL comes with a Bertrand Lens that projects an interference pattern formed at the objective rear focal plane into focus at the microscope image plane It is
4. at the center of the Eye Piece crossline Fig 24 d Check that it stays at the center of the Eye Piece crossline when the stage is rotated 360 degree Repeat the centering process if necessary Note Each objective must be centered separately Position 1 Position 1 Position 1 an 7 a ay PE Position 2 Position 2 24 a 24 b 24c 24 0 Extinction Adjustment lt LABOMED e aS Polarizer away from Fig 25 a Light Path gq Conoscopical Observation onoscopi a mage Fig 26 Cross Li nes 1 Remove all compensator specimen amp test plates out of light path 2 Swing in 10x Objective in the light path 3 Swing out Top lens of Flip Top Abbe Condenser from light Path Ref Fig 08 4 Bring preset vibration direction Analyzer plate by moving Analyzer lever to A position Ref Fig 14 Part 3 5 Bring polarizer to light Path Ref Fig 25 loosen polarizer lock screw for free 360 degree rotation of polarizer 6 Rotate polarizer until you achieve complete extinction Lock Polarizer by tightening Polarizer locking screw 1 Bring an objective of your choice between 20x to 100x in the light path Bring the Pol specimen into focus 2 Keep the abbe condenser in its lowest position 3 Swing in Top lens of Flip Top Abbe Condenser in the light path 4 Open the apert
5. degree and locking provision to stop the stage at desired location Lx POL round mechanical stage is based on hard steel ball bearing which provides even and smooth jerk free motion across 360 degree E Centerable revolving nose piece ie Quartz Wedge 1 4 Order AY y gt Fig 10 a LABOMED USA RP POL PLAN ACHR 40X0 65 0 17 9124040 Fig 11 Lx POL comes with a centerable revolving nose piece to compensate optical axis of each objective as they vary from one assembly to another Lx POL Nose Piece is fully equipped with centering mechanism for each objective to make sure that each object is centered to the stage and microscopic optical axis so that the specimen remains in the center when the stage is rotated Movement of the turret is based on hard steel ball bearing which provides smooth and jerk free motion around 360 degree This nose piece is highly precision parfocalized and par centered Fig 10 1 Objective Centering Allen Screws 2 Nose Piece Cover 3 Port for Lamda Plate Centerable revolving turret is also equipped with a Nose Piece cover which provides a click stop lockable port for Gypsum Lambda wave plates fig 10 a This feature provides comfort while using different Lambda Wave Plates It is ideally located in between specimen
6. minimum exertion of the user However some of the risks that the user should keep in mind are i Risk of Infection After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to prevent infection 1 Be sure to remove the specimen before moving this product 2 In case the specimen is damaged by erroneous operation it is important to clean all surfaces that may come in contact with the specimen ii Electrical Hazards To avoid potential electrical hazards when replacing halogen bulb turn the microscope s main switch to the OFF position and disconnect power cord from wall outlet in advance Whenever you replace your microscope bulb allow lamp socket and bulb to cool before touching Electrical Data I General Instructions 1 Install microscope on a sturdy level table or bench and aovid any restriction of air vents in the base of the unit Do not place microscope on a flexible surface as this could result in blocking the air vents and cause overheating 2 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 3 When installing the Microscope route the power cord away from the microscope frame Should the power cord come in contact with the Microscope base the power cord could melt due to overexposure heat 4 Always ensure that the grounding terminal of the Microsco
7. the direction of intensity level J EN EJ Note Never use an adapter between the power cord and the power Electrical GUURI Orati Switch source it will render the microscopes grounding feature ineffective t Fig 20 16 Lx POL Step 1 Disengage the Analyzer and Bertrand Lens by switching the operating lever to O position as shown in Fig 13 Fully open the aperture diaphragm of the condenser by rotating its ring to the extreme left Lower the microscope stage Place a POL specimen on the stage Swing in the 10x objective into working position Raise the Microscope stage using the coarse adjustment knob until you reach its positive stop Use the fine adjustment knob to bring the POL specimen section into focus by lowering down the sage iN TT el Step 2 Swing in the 10x objective into working position Flip in top lens of Flip Top condenser in light path Close the Koehler field diaphragm so that its closed iris leaves image is present within the field of view Use the condenser focusing knob to bring the image into sharp focus Operate the condenser centering screws simultaneously to Center the image of the field diaphragm Ref Fig 22 a b c After centration open the field diaphragm until the Image iris leaves disappears just beyond the field of Oe 22 a 22 b 22 c Step 3 DO Look through 10x Objective amp Eye Pie
8. the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipments as outlined in this instruction manual Maintenance and Care l General Cleaning Your Microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscope working parts lubricated and optics clean e Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe with cleaning cloth slightly moistened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only ina well ventilated room Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent _ _ wo Ill Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance When not using the microscope ensu
9. Stain free optical system 2 The feature to swing in amp out the top lens of the Abbe Condenser in the light path in order to achieve almost parallel illumination wave fronts for low magnification and birefringence observation Also equipped with iris diaphragm as shown in fig 7 respectively 3 Rotate knurled ring to open and close IRIS Diaphragm for respective Objective magnification in order to match numerical aperture This high quality primary polarizer 1 is 360 degree rotatable with fiducial marking 2 for quick identification of Polarized Cross Polarized positions It is ideally located below condenser and with a lockable mechanism 3 to stop polarizer filter at desired position for comfortable observation experience Lx POL Polarizer is also equipped with a mechanism of swing in out the polarizer from the light path Lx POL round stage has both the key feature of rotatability amp centerability Fig 9 Lx POL round stage is equipped with the key features of rotatability and centerability Fig 9 It provides a 360 degree circular rotation which allows the user to study the orientation by coinciding the objective centration with the microscope s optical axis This makes the center of rotation coincide with the center of field of view Rotatibility allows the user to observe the specimen in the diagnol position the brightest position of anisotropy It is equipped with a vernier scale to measure the accuracy of 0 1
10. and lens is a standard feature in the module which provides conoscopic observation Aset of high quality compensators for advanced polarized observation include Gypsum full wave length Quartz Wedge anda Mica 1 4 wave plate Lx POL J Z SAFETY INFORMATION General Instructions 1 Amicroscope is a precision instrument with delicate glass components please handle with care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body when installing the unit 4 Armhandle is provided for carrying the microscope To prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unit to avoid damage to the specimen slide B Safety Symbols The following symbols are found on the microscope For optimal use it is recommended that users understand these symbols and always use the equipment as prescribed Symbol Explanation This surface has a tendency to heat up and should not be touched unless system has completely cooled down injury to the user and or damage to the equipment M Before use carefully read the instruction manual Improper use could result in Warning against risk of electric shock Main switch is ON O Main switch is OFF if
11. ble in field of View The condenser is too low The condenser iris diaphragm ring is closed Raise condenser light Adjust the aperture according to the objective magnification 4 Observation image is hazy or unclear The objective is not engaged in the light path Engage the objective into position until it clicks The objective eyepiece condenser and or specimen glass is dirty Clean glass parts thoroughly with lens tissue and cleaning cloth Immersion oil is not used with an immersion objective Use immersion oil as suggested Bubbles are present in immersion oil Remove the bubbles by agitation The specified immersion oil is not used Use the immersion oil supplied by LABOMED 5 Part of image is defocused The objective is not properly engaged in the light path Engage the objective into position until the nose turret clicks The specimen is not set properly on the stage Set the specimen correctly on the stage and secure using the specimen holder 6 Coarse focus adjustment cannot lower the stage low enough The condenser is too low Raise the condenser 7 Fields of view through both eyepieces is inconsistent The interpupillary distance is not adjusted properly Dioptric compensation for the two eyes is not set The left and right eyepieces are of different magnification Adjust IPD to the appropriate setting Adjust diopter settings Ensure that both e
12. can be quickly referred to in the future This happens when multiple users work with the microscope Fig 31 0 00 ooow 22 Lx POL Adjusting the Diopter Fig 32 Procedure for adjusting the diopter 1 Rotate the right eyepiece to match the markings of your IPD If your IPD is 64 rotate the eyepiece to 64 mark 2 While looking through the right eyepiece with your right eye rotate the coarse and fine adjustment knobs to bring the specimen into focus 3 While looking through the left eyepiece with your left eye rotate only diopter adjustment ring on the eyepiece until specimen is at its best possible focus Using the Eye Guards When Wearing Eyeglasses Use with the eye guards in the normal folded down position This will prevent the eyeglasses from being scratched When Not Wearing Eyeglasses Extend the folded eye guards outwards direction of the arrow to prevent ambient light from entering into your line of vision The condenser is most often used in the highest position If the observed field of view is not bright enough brightness may be improved by lowering the condenser slightly 1 Rotate the condenser height adjustment knob 2 to move the condenser to the highest or desired position 2 The aperture iris diaphragm ring 1 has an objective magnification scale Slide the diaphragm lever left right
13. capable of examining the objective rear focal plane to ensure exact adjustment of the illuminating aperture diaphragm and to view interference images These images form in the objective rear focal plane when an optically anisotropic specimen is viewed between cross polarizer using a high numerical aperture objective condenser combination It is ideally located between analyzer and eye pieces for an easy in and out movement from the light path Lx POL Bertrand lens is also centerable with respect to the optical axis of the microscope and has focusable mechanism for a comfortable viewing experience Fig 13 amp 14 Bertrand Lens Focusing Knob Bertrand Lens Operating Lever Analyzer Operating Lever Gypsum wave Plate Allen wrench for Objective centering Allen screw for Bertrand Lens Centering ON BON Fig 13 Bertand Lens lever 2 in out O position i e away from light path Analyzer Lever 3 in out O position i e away from light path Fig 14 Bertand Lens lever 2 in BL position i e in light path Analyzer Lever 3 in A position i e in light path 14 Lx POL INITIAL SETUP Observation Head Install the observation head using the following procedure 1 Using the 3mm allen wrench provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in the dovetail cavity 2 Mount the Analyzer attachment by engaging the dovetail provided on the attachment into the dov
14. ces cross hair scale Close the me condenser diaphragm until approximately 20 25 of the iris leaves fill the a E Note When changing the objectives adjust the condenser field j VE field of view Adjust the centering of the diaphragm by maintaining Z p condenser centering screws simultaneously with reference to Eye Piece LL DJ l diaphragm with respectto each objective Fig 23 0 cross hair scale Ref Fig 23 a Fig 23 a 17 Objective Centration ICN Swing in 10x objective in the light path Adjust the condenser aperture diaphragm Retrieve the two centering keys Ref Fig 13 Part 5 and insert them in the centering holes Ref Fig 10 Part 1 above the objective you want to center Focus the POL sample 1 ii Bring some prominent point of the specimen to the center of the Eye Piece crossline Fig 24 a Loosen the stage locking thumb screw which will allow the Round Stage to rotate 360 degree horizontally Rotate the round stage 360 degree until the prominent point of the specimen is furthest away from the center of the Eye Piece crossline it may even be outside the field of view Fig 24 b Adjust the image with the centering screws over the objective until the prominent point of the specimen is midway between the center of the Eye Piece crossline Fig 24 c Adjust the specimen so that the prominent point is
15. etail cavity on the microscope arm and tighten the Head Locking Screw 1 See figure 15 3 Remove the Dust Cover Cap from Observation Head Dovetail and mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity on the analyzer attachment 4 Tighten the Head Locking Screw 2 after positioning the Observation Head as desired See figure15 Insert the eyepieces into the ocular tube of Observation Head using the O following procedure Q ON 1 Remove the protective caps from the observation tube See figure16 2 Insert eyepieces into the ocular sleeve for use See figure17 Fig 17 Mounting the Daylight Blue Filter This filter modifies the color of observation light into a natural daylight color Place Blue filter on Koehler Mount Stage Clips Fix Stage Clips on round rotatable stage for holding the pol specimen Connecting Power Cord Attach the power cord and plug it into a grounded electrical outlet 1 Flip the main switch to I ON as shown in Figure 20 2 Rotating the light intensity adjustment knob in the direction of the arrow increases brightness and rotating knob in the opposite mN direction decreases brightness The intensity bar next to the knob e p E o indicates
16. ghted here Eyepieces Intermediate Analyzer Kit Bertrand Lens centering screw Centerable Revolving Nosepiece Polarizing Objectives Specimen Holder Binocular viewing tube 30 inclined Bertrand lens focusing knob Bertrand lens on off Allen keys for 6 objective centerin A ae J g o Parking for additional A wave plates 2 Analyzer on off Port of wave plate Circular stage with vernier Vernier Scale Flip Top Abbe Condenser Stage locking screw O Coarse and x fine focus knob Condenser Centering Screw Polarizer Koehler mount Polarizer locking screw Daylight blue filter Day light blue filter Intensity regulator N Paired Eyepieces Allen Wrench 3mm 6V 20W Halogen bulb Polarizing attachment OPTIONAL ACCESSORIES System Diagram of Optional Accessories iVu 5100 iVu 0 fi Trinocular head WF 10x WF 16x WF 20x l l l RP 2 5x RP 4x l O RP 20x RP 40x SL RP 100x SL Oil Flip Top Abbe Condenser Polarizing kit Blue filter ji l eS Se Bulb 10 Lx POL Installation and Operatio
17. icles away with compressed air of mini dust blower Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton slab To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol Grease must always be removed using a solvent Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above Wipe using a spiral movement Donot use a zig zag motion Cleaning of Painted Surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose 3 tealth Risks This microscope has an ergonomic design that ensures
18. ight blue filter Koehler mount Intensity regulator Lx POL Trinocular Eyepieces Intermediate Analyzer Kit Bertrand Lens centering screw Centerable Revolving Nosepiece lt gt e viewing tube 30 inclined Bertrand lens focusing knob Bertrand lens on off Allen keys for f a objective centering Parking for additional wave plates 2 0 Analyzer on off Lx POL Polarizing Objectives Specimen Holder i Port of wave plate Circular stage with vernier Vernier Scale Flip Top Abbe Condenser Stage locking screw Ke Condenser Centering Screw Polarizer Koehler mount Coarse and gt C fine focus knob Polarizer locking screw Day light blue filter LABoMep Intensity regulator Lx POL UNPACKING YOUR MICROSCOPE Power Cord Observation head Eyepieces Lx POL STANDARD COMPONENTS After removing your microscope from its packaging make sure that all of the following contents are present Please note that the contents of your microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highli
19. n of Optional Accessories iVu Camera Module System 1 Mount the Video adapter 1 2 part 3143300 912 Fig 4 on Trinocular observation head Fig 5 2 MountiVu Camera Module System Fig 3 on video adapter Fig 3 Fig 4 Fig 5 Optional Eyepieces 10X eyepieces are provided To replace 1 Pull out the 10x eyepieces out from the observation heads ocular tube 2 Insertdesired eyepieces in empty ocular tube e a O Lx POL DETAILED INTRODUCTION OF ASSEMBLIES Flip Top Abbe Condenser Top Lens Gz Wtididitititibititiiit a Iris shifter Top lens in Light Path Top lens out from Light Path Fig 7 Polarizer TE e 1 H IS Polarizer out of Light Path ames 3 s Round Stage Lx POL Lx POL Flip Top Abbe condenser is has three very important basic requirements i e 1
20. nd lens is away from light path Bring Betrand Lens in light path at BL position Condenser Top Lens not in the light path Swing in Top Lens of condenser in the light path Using lower magnification objective Go to specified objective magnification i e from 20x to 100x 13 Extinction failure Analyzer amp Polarizer out of the light path Bring Analyzer amp Polarizer in the light path Analyzer amp Polarizer are not in cross positions Adjust Polarizer by rotating to get complete extinction SPECIFICATIONS 1 Illumination Built in illumination system Halogen 2 Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 3 0um per graduation Fine adjustment stroke 0 3mm per turn Total stroke 12 7mm Co axial coarse and fine focusing on ball drive 3 Revolving nosepiece Quadruple positions fixed Reverse angle 4 Observation tube Binocular Trinocular Field number 20 Standard 20 Standard Tube tilting angle 30 30 Interpupillary distance 48 75 48 75 adjustment range 5 Round Stage Size Dia 160mm Rotatability 360 degree Specimen holder Spring Loaded stage clips 6 Condenser Type Flip Top Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions amp Weight 405mm L x 210mm W x 425mm H 7 kg net 8 Electrical Halogen 6V 20W upto 2 000 hours 9
21. ndenser If oil is not placed there the observed image may appear dark 5 After use remove oil from the objective front lens by wiping with lens tissue slightly moistened with an ether 70 alcohol 30 mixture Caution If immersion oil makes contact with your eyes rinse eyes out thoroughly with fresh water If immersion oil makes contact with skin wash affected areas with soap and water If prolonged discomfort is experienced consult your physician immediately La aD TROUBLESHOOTING GUIDE a ww Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take corrective action as needed If problem persists please contact LABOMED or your local LABOMED dealer Observation Cause Remedy 1 Uneven brightness in observation field The objective is not engaged in the light path Engage the objective into position until the nose turret clicks The condenser is too low Raise up to achieve more light The objective eyepiece condenser and or window lens are dirty Clean them thoroughly as previously prescribed in Optical Cleaning 2 Dust or stains are visible in observation field The eyepiece condenser window lens and or specimen glass is dirty Clean glass parts thoroughly with lens tissue and cleaning solution prescribed in Optical Cleaning 3 Glare visi
22. of LABOMED a Lx POJ To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended CONTENTS CONTENTS 10 CENTRATION 17 19 a Preparation for centration b Kohler illumination centration c Flip Top Abbe Condenser centration d Objective centration e Extinction adjustment f Conoscopical adjustment 11 SUMMARY OF POLARISED OBSERVATION PROCEDURE 20 12 SUMMARY OF POLARISED OBSERVATION PROCEDURE 21 24 a Placing the specimen on stage b Adjusting the focus c Adjusting the Interpupillary Distance d Adjusting the Diopter e Adjusting the Condenser position and Aperture Iris Diaphragm f Switching the objectives g Using the 100x Immersion Objective 13 TROUBLESHOOTING GUIDE 25 26 14 SPECIFICATIONS 27 INTRODUCTION The Lx POL is a research polarizing microscope reflecting a modern design as well as the latest in optical and mechanical advancements Designed for professionals this microscope offers many features and functions for a diverse set of applications It is a truly professional polarizing Microscope that meets and indeed exceeds the quality of some of the competing Microscopes Here are a few points highlighting the benefits of the Lx POL Extra clarity and contrast is provided through a 360 degree rotatable viewing body inclined at 30 deg
23. or Fuse replacement Set the main switch to O OFF disconnect the power cord from the wall outlet Before replacing the fuse remove the parts that may drop such as the filter and specimen from the microscope frame Turn around the microscope to its back so thatthe AC inlet is visible 1 Use a flat head screw driver to open the fuse holder 1 2 The fuse tray will come out with 2 live fuse and 3 spare fuse Do not pull out the fuse tray with force as it is locked and will not be out completely 3 Replace the primary fuse 2 with the spare fuse 4 Engage the fuse tray back in Always use the designated Fuse Using a fuse other than those specified by LABOMED may lead to a fire hazard 5 Lx POL Binocular Binocular viewing tube 30 inclined A Bertrand lens focusing knob Bertrand lens on off Allen keys for 6 ee objective centering Bertrand Lens centering screw o Parking for additional wave plates 2 Eyepieces d Intermediate Analyzer Kit Analyzer on off Port of wave plate Centerable Revolving Nosepiece 2 Circular stage Polarizing Objectives with vernier Specimen Holder Vernier Scale Stage locking screw Flip Top Abbe Condenser 0 Coarse and Condenser Centering Screw E fine focus knob Polarizer Polarizer locking screw Day l
24. pe and that of the wall outlet are properly connected If the unit is not grounded LABOMED can not warrant electrical safety 5 Never allow metallic objects to penetrate the air vents of the Microscope frame as this could result in user injury and damage to the Microscope 6 After operation of Microscope be sure to disconnect power cord from connector socket of the Microscope or from the wall power outlet il Bulb Replacement Ill Installing or Replacing the Fuse 1 Before attaching the lamp bulb remove the parts that may drop such as the filter and specimen from the Microscope frame and place the Microscope on its back so that the bottom plate is exposed 2 Pull the lock know 1 on the bottom to open lamp housing door fig 1 3 Hold the halogen bulb 2 without taking it out of the polyethylene bag so as not to taint the bulb with fingerprints and push the bulb into the pin holes on the socket 3 After attaching remove the polyethylene bag 4 With the lock knob pulled out close the lamp housing door then push the lock knob back to lock the cover Always use the designated bulb Using a bulb other than those specified by LABOMED may lead to a fire hazard Fingerprints or stains on the lamp bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alchohol Applicable Bulb 6V20W Halogen Bulb P N CX 013 Caution F
25. re that the frame is fully cooled before storing the unit in a dry locker or covering with a dust cover provided To clean the Flip Top Condenser fully loosen the securing thumb screw 1 and remove the condenser then wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue The Flip Top Abbe can be re attached in its seat by tightening securing thumb screw and raising condenser bracket to desired position As shown in picture I 5 Be sure to observe you local rules regulations for product disposal Always cover the Microscope with the provided dust cover when not in use Optical Cleaning The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage To clean the lens surfaces remove dust using a soft brush or compressed air cans available at your local electronic store For removing finger marks or grease soft cleaning cloth or lens tissue lightly moistened wit cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use Meathanol Observe sufficient caution in handing Methanol Place the Objectives and or eyepieces on a dust free surface e g aluminium foil All other optical components to be cleaned should be as accessible as possible Blow all loose dust part
26. ree with IPD adjustments The pressure die cast stand consists of ball bearing frictionless sideways focusing to avoid any loss in motion The sturdy new stylish design provides a high degree of comfort as well as stability The high powered objectives are spring loaded to prevent accidental damage to specimen slides The highly precision Parfocalized amp Parcentered reverse angle quadruple nosepiece has the provision of centering for all objectives The round ball bearing Rotatable Stage has smooth 360 degree travel and has 1 degree graduations on the scale which provides accurate location of the specimen High power illumination is delivered through our well crafted Universal Power Supply and operates on 100V 240V AC constantinput Halogen bulb 6V 20W has an average life span of up to 2 000 hours The Lx POL is equipped with a Flip Top Abbe Condenser N A 1 25 anda swing in and swing out provision for the Top Lens to attain for brighter illumination levels An iris diaphragm is also provided for better resolution and contrast control High quality Polarizer amp Analyzer filters for perfect extinction level Polarizer This is provided below the condenser and is 360 degree graduated and lockable in any desired position Analyzer The advances Analyzer module is located below the viewing tube and provides specific cross polarization through the full 90 degree quadrant Acenter adjustable focusable Bertr
27. tage 3 When coarse focusing of the specimen is obtained an image is observed rotate the fine adjustment knob 2 for fine detail Fig 30 focusing Working Distance WD The WD refers to the distance between each objective and the specimen when acute focus of the specimen is obtained Objectives Eye Piece Flip Top Abbe 10x 20 W F 4140010 Condenser Objective N A WD Cover Resolution Total Field of Depth of Obj Flip Position Magnification mm Glass um mag view mm focus um N A In Out 2 5x 9124002 0 08 20 0 0 17 5 0 25x 8 0 400 0 08 Out 4x 9124005 0 10 30 0 0 17 3 36 40x 5 0 200 0 10 Out 10x 9124010 0 25 4 04 0 17 1 34 100x 2 0 30 0 25 Out 20x 9124010 0 45 1 10 0 17 0 75 200x 1 0 6 0 45 In 40x 9124010 0 65 0 45 0 17 0 52 400x 0 50 3 0 65 In 100x oil 9124010 1 25 0 14 0 17 0 27 1000x 0 20 0 70 1 25 In Adjusting the Interpupillary Distance IPD The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it
28. to achieve the desired illumination level 3 Swing in the flip top condenser on 40x and 100x magnification The flip top condenser should be in swing out position on 4x and 10x objective position 23 G Switching the Objectives Ro pay a 0 aara Q USA RP POL PLAN ACHR 40X 0 65 0 17 9124040 aN Using the 100X Immersion Objective Fig 35 Rotate the revolving nosepiece 1 so that the objective to be used is in line above the specimen Always use the knurled surface to rotate the objective nosepiece The designated immersion oil should be in contact with the cover lens of the 100X immersion objective If not the specimen will appear distorted and dull Itis recommended that LABOMED immersion oil is always used Immersion Process 1 Bring the specimen in focus using first the 10x then 40x objective 2 Disengage the 40x cycling towards 100x and place a drop of immersion oil on the center point of the specimen 3 Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condenser of this microscope manifests the full performance when oil is placed between the slide glass and the front lens of co
29. ure diaphragm 5 Swing in lever of Bertrand lens to BL Ref Fig 14 Part 2 and focus image by rotating Bertrand Lens focusing knob Ref Fig 14 Part 1 Note If the conoscopic image is dark then move the condenser upward to find the suitable position where the image is brightest Conscopic image may not be at the centre of Eye piece cross line intersection However it will have no measurable effectdue to universal infinity optical design SUMMARY OF POLARIZED LIGHT OBSERVATION PROCEDURE Flip the main switch to ON Disengage Analyzer and Bertrand Lens lever to O position TES Fuse Holder Main switch Engage the 10x Objective in the light path Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting Adjust Koehler aperture iris diaphragm AA Adjust Flip Top Abbe Condenser aperture iris s Bring the specimen in focus amp adjust Flip Top Condenser in amp out position Adjust objective cent ration and stage cent ration with respect to Eye Piece cross hair scale Engage Polarizer amp Analyzer filters in the light path and use Bertrand Lens and Wave plates Adjust the light intensity y Observe Specimen Lx POL S LABoMep Lx POL
30. yepieces are of are of same magnification LABOMED does not recommend using third party eyepieces in conjunction with LABOMED eyepieces Lx POL Observation Cause Remedy 8 Objective hits the specimen when an objective is switched to a higher The specimen slide is upside down Set the specimen correctly with the cover glass facing upwards magnification objective The cover glass is too thick Use a cover glass with thickness of 0 17mm The stage is raised too high Lower the stage The slide has slipped from the slide holder Re position the slide in the slide holder Slide is of excessive thickness Use slides with thickness between 0 9 and 1 4mm 9 Bulb does not turn On Bulb is not mounted Attach a bulb Bulb is blown Replace the bulb The power cord is unplugged Not firmly secured Ensure power cord is securilly plugged into the box socket wall outlet Fuse is blown Check and replace with live fuse 10 Bulb blows easily The specified bulb is not used Replace with the specified bulb 11 Field remain dark even Bulb is on In the Exintetion Position Remove Analyzer from light path by switch Analyzer lever to out O position Swing out Polarizer from light path Bertrand Lens in the Light Path BL Swing out Bertrand Lens to out O position i e away from light path 12 Conoscopic image is not visible Bertra
Download Pdf Manuals
Related Search