OTMO-0023
Contents
1. when prompted at the time of the analysis Immunohisctochemical staining IHC should be performed on sections of the O STANDARDS block and the test samples in one staining batch using the same reagents and staining procedure and on the same instrument Do not overstain your sections Titrate your antibody first on sections of the O STANDARDS and select the lowest antibody concentration that produces strong staining intensity in the positive cells while showing completely negative staining in negative cells then use this antibody dilution in the IHC staining for the quantitative analysis Use hematoxylin counterstain that stains nuclei light blue but not too weak control negative nuclei should have enough blue stain to stand out clearly from the background When capturing TIFF images of the O STANDARDS slides capture an image of an area with the highest percentage of positive cells using a Plan Apo or similar quality 20 X microscope objective and label the image with the lot number followed by A for example 201 p53 A for the cells in the O STANDARDS section that stained positive for p53 then similarly take an image of the negative cells in the O STANDARDS section and label it with the lot number followed by B Please read the OTMIAS user manual before doing IHC staining of O STANDARDS Watch the demo videos if available Copyright 2015 by Olive Tree Media LLC Please refer to terms of sale on our website www2. Catalogue number OTMO 0023 O STANDARDS for p53 protein overexpression Assay by immunoperoxidase staining LOT numbers 201 p53 What is supplied One paraffin block containing two core one cell line with mutant p53 and p53 protein overexpression positive control and tonsil tissue or a cell line with wild type p53 negative control both have been fixed in 10 buffered formalin overnight at room temperature then processed on a regular histology processor cycle no microwaving and embedded in paraffin When immunostained with antibodies to p53 tested with antibodies DO 7 and BP53 12 1 one of the cores will be positive cell line and the other tonsil or cell line will be negative for p53 See image in the O STANDARDS Image Gallery on our website Use Use as a reference standard when determining the presence and extent of p53 protein overexpression in sections of formalin fixed and paraffin embedded cell lines or tissues Samples must be fixed and processed similar to this O STANDARDS block For optimal results sections of O STANDARDS and the sample to be analyzed should be cut at the same thickness at the same time on charged glass slides and heated in oven to melt paraffin Always write the lot number corresponding to the marker to be analyzed on the slides exactly as written in the corresponding product data sheet you will need to enter the appropriate lot number corresponding to the biomarker being assayed in OTMIAS
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OTMO 0023