User Manual for 320A
Contents
1. Gin E A sia f 1 2 1 Confocal II 31 0112 aoo 00W PET T 1 2 2 Single Wire Myograph 3 EA n Chapter 2 Setting UP cc n 9 2 1 Setting up the complete Wire Myograph System c ssccsccsssssseceeecccsesseeceeccccesssesesececesseeseeececesesasesececesesetessceccecsssetececeseestseesoes 9 PAM Mee MES CTO MOS Cara anann a i 10 Chapter 3 The wire Interface Menug c ssscccssssseccennsseeceensseeceensseeneensseceennsseeeonnaseeneonssseeeeassseneenssecennnssseconnsseeneenssseseenssseneenses 11 3 1 General description on how to navigate 50186 Ru dunaknanndduiantcncsincceenissliesus 11 ouo POWO UP OI aiena E stems E E BU E O GUN DD UE 11 R EE EE E A E I E AA na dele A E A A EA 12 IS FU A PE A AEE N A IA ai A E A A AA EAN AT AN D DEA E A AE A Gach EEAS A E N 12 VAS E A N A N EA E 13 eo HME E O a T3 Meet UNE Eom AA A N A A AAA A NEE EA E E T E A ET 14 INU aoi a AS 14 JLA PA INO FAO EE2. OmN and 2 5V 100mN NOTE REMEMBER TO PRESS ENTER TO SAVE THE LINE CHANGES IN MEMORY Step no The pH electrode is now calibrated if OK go to next step else repeat the calibration OH value 4 00 Probe Temp 25 0 NEXT STEP SELECT ANALOG OUTPUT Select output 1 SELECT Select output 2 SELECT Select output 3 SELECT Select output 4 SELECT OUTRO Output 1 Force SELECT Setting 1 2 5V 100mN SELECT Setting 1 2 59 100mN SELECT o UIS TES Output 1 Force SELECT Setting 1 2 5V 100mN SELECT Setting 1 2 5V 100mN SELECT CHAPTER 3 19 3 7 4 Interface Settings The Interface Settings sub menu has an additional three sub menus Temperature Difference pH Set up Factory Diagnostics 3 7 4 1 Temperature Difference offset The temperature difference function allows the user to fine tune the tem perature set point of the system Although the temperature set point for the system can be set in the Heat Menu there may be a small discrepancy between the actual temperature of the system and the defined set point The user can adjust the temperature of each chamber individually to fine tune the temperature setting so the exact temperatures are achieved in each chamber This is referred to as a temperature offset TEMP OFFSET ON CHAMBER The SELECT and ALL functions are the same in this menu as for previously described menus Pressing ENTER will store the numbers in memory
3. supportedmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Te 566 0 21 5425 1330 Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com support dmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
4. 18 3 3 Select Analog Output ODIO aN 5 a a A AEAEE Sa AONA A Aaa A Aa 19 A E OU NR TTE E E RE 20 3 7 4 1 Temperature Difference offset RMM A fMfE UU 20 PH OEP e 20 NS Tm 20 Appendix 1 System specifications ssvnciisivccetsnscacessnnscsessensecnnssisectuancsssnnsnsveessbsensiedeusswenssossnednansssenudaneeceusadeecsuohsswensnansieneusivecnuannenssn 21 INS oe ee ners eee oe ete eater cc eee eee eine dee peau wea A ae dere eee en wo 23 CONTENTS 3 SAFETY The Wire Myograph System has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be used for these purposes Nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the apparatus the internal electronics pose a risk of electric shock Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus e Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Do not install near any heat sources such as radiators h
5. for future experiments 3 7 4 2 pH Set up Menu The pH calibration is a 2 point calibration In the pH set up menu the two pH values used for the 2 point pH calibration should be selected The default in the pH Calibration setup is the pH values 4 and If using pH buffer with different pH values than 4 and 7 for the pH calibration enter the appropri ate pH The temperature is an important parameter in the calibration formula and is obtained automatically if the temperature compensation it is set to AUTO as shown If MANUAL is chosen a manually set temperature is used in the pH calibra tion formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffer is measured with a thermometer and entered manually in the Manual temp Value line 3 7 4 3 Factory Diagnostics Entering Factory Diagnostics will display the Login code to diagnostics window This window is only for trained technicians and used for diagnos tics and troubleshooting purposes The general user will not have access to this window Entering the proper five digit login code however will allow the trained technician access to diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer 20 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL INTERFACE SETTINGS TEMPERATURE DIFFERENCE SET UP FACTORY DIAGNOSTICS TEMP OFFSET Chamber 1 2 C B
6. 1 3 Confocal Wire Myograph 360CW with close up of the chamber CHAPTER 1 7 1 2 2 Single Wire Myograph 320A Port for connection to Wire Interface Transducer house SS Ty e tie Micrometer Allen screws for fine alignment of the wire jaws 2 on each side Pipe for gas supply C Tm Pipe for filling the chamber using the 40 mm funnel Suction pipe for connection to vacuum pump 0 06000000000000000000000040000000000 999999 eter emen e Force transducer pin Wire jaw connected to amp TTA BM e Wire jaw connected to force transducer micrometer 0 00000000000000000000000000000000000000 999999 9999999999 9899999696 Window at the bottom of the chamber for imaging under the wire jaws Figure 1 1 Single Wire Myograph with close up of chamber 8 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL CHAPTER 2 SETTING UP 2 1 Setting up the complete Wire Myograph System Wire Interface rear panel POWER pH Meter Power supply Wire Myograph amp r 2 yoprap Data Acquisition System via BNC cables or USB Oxygen Carbogen Compressed air Gas supply manifold Vacuum Pump Suction Bottle Figure 2 1 Example of a complete Wire Myograph System 320A Figure 2 1 is an example of a complete set up for the Wire Myograph System 320A A set up for Confocal Wire Myograph would look the same but with a Conf
7. 6 C SET TIMER Set Timer 00 00 00 SET TIMER Set Timer 00 00 00 CHAPTER 3 13 To activate the buzzer press ON and it will turn green SET TIMER In Main Menu the bell icon also turns green Q ON Set Timer 00 00 00 SELECT UB 3 7 Settings Menu The Setting Menu contains several sub menus These sub menus include SETTINGS Force calibration pH calibration FORCE pH RECORD Record out select CALIBRATION CALIBRATION OUT SELECT Interface settings INTERFACE SETTINGS 3 7 1 Force Calibration Menu Prior to shipping the Wire Myograph System has gone through two days of continuous testing including final force calibrations However DMT recommends that new force calibrations are performed before starting to use the Wire Myograph System for the first time Begin the calibration procedure by pressing Force Calibration in the Settings Menu The calibration procedure is listed in 6 indi vidual steps and needs to be performed to calibrate the system Before starting the Force calibration the following is performed e Place the calibration bridge and T balance on the Wire Myograph see figure 3 1 for 360CW or 3 2 for 320A allowing it to be warmed up together with the Wire Myograph Turn on the heating in the Heat sub menu to the appropriate temperature used during your experiments e Mount a 40 um stainless steel wire on the jaws connected to the force transducers NOTE THE WEIGHT SHOULD NOT BE PLACED
8. DMI DANISH MYO TECHNOL Ost WIRE MYOGRAPH SYSTEMS 360CW AND 320A USER MANUAL User Manual Version 1 0 TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 35 are trademarks of ADInstruments Pty Ltd DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the Wire Myograph system No part of this document may be reproduced by any means without the prior written permission of Danish Myo Technology A S Copyright Danish Myo Technology A S DANISA MeO TECHNOL OGS 2 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL CONTENTS ee pe re ne ce enact EE ce ee 2 ERO IL D o oo G Al EC Declaration OF ConFOrmilEy eicussssnnesencansuavisndexnennasiverusvecvdseunesicensensneceeies d Rc ow D c Er On x VD OR OR DNE a Chapter cc c 6 1 1 Wire REM EFI SEIN 6 WIV O ON
9. ON THE T BALANCE YET Step 1 Involves setting up the chamber for calibration Fill the chamber to a normal level with double distilled water Move the jaws apart FORCE CALIBRATION Press NEXT STEP Step no 1 Follow the weight calibration procedure in the User Manual Fill the chamber with double IMPORTANT distilled water TURN ON THE HEAT AND SET THE TEMPERATURE AT THE LEVEL AT WHICH __ When ready go to next step THE EXPERIMENTS WILL BE PERFORMED 14 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL Figure 3 1 Force calibration set up showing placement of the calibration bridge and T balance with weight in place for Wire Myograph 360CW The 2 g weight is placed on the pan Figure 3 2 Force calibration set up showing placement of the calibration bridge and T balance for Wire Myograph 320A CHAPTER 3 15 Step 2 Involves setting up the Calibration Kit for the actual force calibra tion Make sure that the T balance pin is placed between the wire and the jaw as illustrated in figure 3 3 Carefully move the calibration bridge until the tip of the T balance pin is placed freely between the wire and the jaw which means it does not touch the wire or the jaw see figure 3 3 Press NEXT STEP when the calibration kit has been properly placed Step 3 Initiates the heating process for the chambers if not already done In order for the calibration to be accurate the transducers must be heated to the experi
10. UAL Step 4 The calibration is now finished The readings in the two bottom lines are the actual pH and temperature readings 3 7 3 Select Analog Output optional The Analog Output Menu determines what will be sent to the BNC analogue output REC 1 REC 2 REC 3 REC 4 at the back of the Wire Interface There are 4 analog outputs and each output is individually programmable Any change made to the analog output will affect the data sent from the Wire Interface to a data acquisition system such as AD Instruments PowerLab Therefore remember to check the data acquisition system s set up when a change is made The analog output is working in range of 2 5V to 2 5V Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up Use SELECT to select the line to be changed Use the UP and DOWN arrow or DEFAULT to change select a new set up The following parameters in the Wire Interface can be selected as output to the analog channels Force Near Force Far Temperature Probe Chamber Temperature pH1 Optional Digital Output 1 Digital Output 2 Digital Input 1 Digital Input 2 When the chosen parameter is selected press ENTER to save the value in the memory The output range is 2 5V to 2 5V The user can select the parameter value that gives 2 5V and the value that gives 2 5V In this example LOOMN is 2 5V and 100mN is 2 5V Selecting asymmetrical values is also valid They can be 2 5V
11. brations 12 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL 3 5 Heat Menu The heating temperatures are controlled from this menu To turn the heat on or to change the temperature for the system press HEAT in the Main Menu The Heat Menu will be displayed allowing the user to change the system temperature as well as turn the heat on or off Pressing DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing UP or DOWN arrows Pressing ENTER will save the new temperature setpoint ON To turn the heat on press ON and it will turn green The system will heat to the desired temperature setpoint In the Main Menu the heat icon turns green when the heat is on 3 6 Timer and Buzzer Menu The timer and buzzer are controlled from this menu Use SELECT to program the timer The timer can be programmed in the interval from O to 23 59 59 Hours minutes seconds When Set Timer is selected use the UP and DOWN arrows to program the timer Use the RIGHT arrow to move from hours minutes and seconds Press ENTER to save the programmed time To start the timer press ON and it will turn green When the timer reaches zero it automatically switches to OFF If the buzzer is turned on it will make a tone when the timer reaches zero SET CHAMBER TEMPERATURE Temperature setpoint 37 0 C Probe temperature 36 6 C Temperature setpoint 37 0 C Probe temperature 36
12. d If MANUAL is chosen the Manual temperature is used in the pH calibration formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffers is measured with a thermometer and entered manually in the Manual temperature line The calibration procedure is listed in 4 individual steps and needs to be performed one by one to calibrate the pH Step 1 Involves cleaning the pH electrode and the temperature probe with pH CALIBRATION double distilled water When ready press NEXT STEP Seo no BOOB Follow the pH calibration procedure in the User Manual Clean the pH electrode and the temperature probe When ready go to next step NEXT STEP Step 2 Place the pH electrode and temperature probe in the high buffer solution here pH 7 and turn on stirring When the relative pH output inthe pH CALIBRATION blue line is stable press NEXT STEP Ste no BOF Apply buffer with the High pH value to the pH and temp probe When the pH reading is stable go to next step pH readings 1863 Step 3 Place the pH electrode and temperature probe in the low buffer solution here pH 4 and turn on stirring When the relative pH output in the pH CALIBRATION blue line is stable press NEXT STEP som OSG Apply buffer with the Low pH value to the pH and temp probe When the pH reading is stable go to next step pH readings 883 NEXT STEP 18 WIRE MYOGRAPH SYSTEM 360CW 320A USER MAN
13. e E 9 9 will only detect 1 gram or 9 81 mN of force The weight placement should When the relative force reading cause a positive increase in the four digit number Wait at least 30 to 45 is stable go to next step seconds for the applied force to stabilize before pressing NEXT STEP Force Chamber 3346 NEXT STEP Step 6 is to verify that the calibration was performed correctly The Force FORCE CALIBRATION Chamber reading should be 9 81 0 1 mN If the Force Chamber reading is off by more than O 1 mN then remove the weight press BACK to return to S EB 2 3 4 2 tep no 1 2 3 4 Step 4 and repeat the calibration process If the Force Chamber reading is E E satisfactory then press NEXT STEP to end the calibration The transducer is now calibrated Force read out should be 9 81 mN 0 1 mN If OK go to next step Otherwise repeat the calibration NEXT STEP After calibrating the force transducer carefully remove weight T balance and calibration bridge The Wire Myograph System is now ready for force measurements Force Chamber 9 81 mN CHAPTER 3 17 3 7 2 pH Calibration Procedure Before the pH calibration is performed be sure to select the way the pH electrode is intended to be used See pH Set up Menu under Interface Settings in chapter 3 7 4 2 The temperature is an important parameter in the calibration formula and is obtained automatically if the Temperature com pensation has AUTO selecte
14. eat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time The Wire Interface is delivered with an external 100 240VAC to 24VDC adapter Protect the power adapter and cord from being walked on or pinched Particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped EC DECLARATION OF CONFORMITY Danish Myo Technology A S Certify and declare that the following apparatus Wire Myograph System DMT360CW DMT320A Restrictive use Only for laboratory use Manufactured by Danish Myo Technology A S Skejbyparken 152 8200 Aarhus N Denmark Conforms with the essential requirements of the EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to source
15. ed before using the Wire Myograph System The force calibra tion procedure is described in detail in chapter 3 7 1 10 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL CHAPTER 3 THE WIRE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Wire Interface 3 1 General description on how to navigate the touch screen Menus on the Wire Interface are all accessible by a touch screen To access a menu simply touch the screen When a setting needs to be changed TEMP OFFSET press SELECT beside the line to be changed Chamber 1 20 SELECT SELEC Bottle LTG SELECT The line to be modified will turn blue indicating that the Wire Interface is waiting for input When ALL is chosen all lines corresponding to all four channels will turn blue ALL Changing the numeric value for the chosen parameter can be done by pressing UP or DOWN arrows AJY Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory ENTER Pressing the white X in the red box will exit the menu and take you automati cally to the Main Menu MULTI INTERFACE 3 2 Power up screen After turning on the Wire Interface an introduction screen appears It dis Multi Myograph System plays the product system model no software version number date and the Model 360CW 320A system ID number Software Revision 02 04 The sys
16. f the USB ports on the computer CHAPTER2 9 3 Turn on the Power Turn on the power to the Wire Interface at the power switch and then turn on the computer Start the data acquisition pro gram on the computer and the Wire Myograph System is now ready for use 4 Gas supply Connect the pipes for gas supply on the Confocal Wire Myograph 360CW chamber cover see figure 2 3 to an adjustable gas supply using thin silicone tubing For the Wire Myograph 320A connect the pipe for gas supply on the Wire Myograph side see figure 2 4 5 Suction connection Connect the suction pipe on the Confocal Wire Myograph 360CW chamber cover see figure 2 3 to a vacuum pump via a suction bottle and the electronic vacuum valve optional For the Wire Myograph 320A connect the suction pipe on the Wire Myograph side see figure 2 4 6 pH electrode optional Connect the pH electrode to the pH port on the rear of the Wire Interface Pipes for gas supply Suction pipe for connection to vacuum pump Figure 2 3 Chamber cover for for Confocal Wire Myograph 360CW vo Pipe for gas supply Suction pipe for connection Pipe for filling the chamber to vacuum pump Figure 2 4 Pipes on the side of Wire Myograph 320A 2 2 The first force calibration Prior to shipping the Wire Myograph System has gone through two days of continuous testing including final force calibrations However DMT recommends that a new force calibration is perform
17. four digit number Wait at Step no og Make sure that the transducer is least 30 to 45 seconds until the four digit number is stable before pressing NEXT STEP not subjected to any force When the relative force reading NOTE is stable go to next step NORMAL OPERATING VALUES FOR THE FORCE TRANSDUCER DURING CALIBRATION SHOULD BE BETWEEN 3000 AND 3500 IF THE VALUE Force Chamber 3261 IS 0 A SINGLE DIGIT OR A THREE DIGIT NUMBER THE FORCE TRANS DUCER IS BROKEN AND NEEDS TO BE REPLACED IF THE VALUE IS LESS THAN 2000 OR GREATER THAN 4500 BUT STILL A FOUR DIGIT NUM BER THE FORCE TRANSDUCER IS BROKEN BUT SHOULD BE ABLE TO BE REPAIRED BY A TEMPERATURE COMPENSATION DONE BY DMT IF THE MESSAGE OFF IS DISPLAYED ON THE MAIN PAGE OF THE INTER FACE EVEN THOUGH THE CHAMBER IS PLUGGED IN AT THE REAR OF THE WIRE INTERFACE THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED IN ADDITION IF THE FORCE READING S APPEAR YEL LOW CANNOT BE RESET TO ZERO AND THE TRANSDUCER CANNOT BE RECALIBRATED THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED Step 5 At this step place the 2 gram weight at the pan on the calibration T balance closest to the transducer over the transducer see figure 3 1 or FORCE CALIBRATION 3 2 to simulate the stretch created by the contraction of a mounted ring preparation Step no 1 JO Carefully place the 2 g weight Remember a 2 gram weight in a 90 vector is divided and the transducer on th
18. grease for protection of linear slides e 4xspare screws 360CW for mounting of jaws or x spare screws 320A e 1x screwdriver e 10x Spare cover glass od 12 0 x 0 17 mm Optional Accessories e PowerLab e LabChart e DMT Device Enabler e Stimulator e pH meter e Vacuum Pump e Electronic vacuum valve e Waste bottle e Gas supply manifold UNPACKING THE WIRE MYOGRAPH SYSTEM 5 CHAPTER 1 SYSTEM OVERVIEW 1 1 Wire Interface front and rear Touch display Power indicator Figure 1 1 Wire Interface front Analogue recorder output connection to Grounding connect to external data acquisition software optional Data Acquisition ON OFF switch e MYOGRAF REC 4 RE WP 5 24V DC port e e for power G J REC 2 REC USB connection port Connection port for for computer Connection port Wire Myograph for pH electrode optional Figure 1 2 Wire Interface rear 6 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL 1 2 Wire Myographs 1 2 1 Confocal Wire Myograph 360CW 25 pin serial cable for communication with Wire Interface Vertical micrometer screw Horizontal micrometer e Allen screws for fine alignment of the wire jaws Wire jaw connected to fel aaa Wire jaw connected to horizontal micrometer Force transducer pin Window in the base of the chamber for imaging Figure
19. mental temperature to be used This accommodates heat in duced expansion of the electronic parts in the transducer Otherwise inac curate readings and transducer drift may occur producing errors into the experiment Covering the chamber with the chamber cover will expedite the heating Place the temperature probe into the chamber for the first calibration to determine when the chamber has reached the target temperature Heating will take about 20 to 30 minutes for the chamber and transducer to reach 37 C with the chamber cover in place Once the chamber are heated and have reached the target temperature press NEXT STEP FORCE CALIBRATION Step no o B 5 Place the calibration bridge on the myograph Place the bridge so the pin does not touch the wire jaw When ready go to next step FORCE CALIBRATION NEXT STEP Step no 5 5 Turn the heat on Wait until the temperature is stable Temperature setpoint 37 0 C Probe temperature 36 8 C Figure 3 3 Illustration of how to fit the T balance pin between the wire and the gap in the jaw support 16 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL NEXT STEP Step 4 is the first step in the actual force calibration process A four digit number will be displayed in blue at the bottom of the screen If nothing has FORCE CALIBRATION been disturbed during the heating process the zero O gram or 0 0 mN calibration should be stable as indicated by the
20. ocal Wire Myograph instead The set up includes optional equipment such as pH electrode for measuring pH in the buffer an electronic vacuum valve suction bottle vacuum pump a computer and Data Acquisition System IMPORTANT TO RECORD A NOISE FREE SIGNAL IT IS IMPORTANT TO CONNECT THE WIRE INTERFACE GROUND CONNECTION TO THE DATA ACQUISITION SYSTEM S GROUND CONNECTION 1 Wire Myograph to Wire Interface connection Connect the Wire Myograph to the Wire Interface using the grey 44 25 pin connector cable The end of the cable with the temperature probe is attached to the Wire Myograph 2 Wire Interface to computer connection Data acquisition is possible either by connecting the Wire Interface directly to a computer or through a Data Acquisition System able to collect voltage output 0 2 5volts such as a PowerLab Data Acquisition System or similar system Direct computer USB connection for Labchart AD Instruments users latest version of Labchart Connect the Wire Interface to the computer with the USB cable from the back panel to the USB port Analog Data Output through a PowerLab or similar Data Acquistion System optional Connect the Wire Interface to the analog data acquisition system using BNC cables Connect Rec 1 Chamber 1 on the Wire Interface to Input 1 on the analog data acquisition system Rec 2 Chamber 2 to Input 2 on the analog data acqui sition system 2 and so forth Connect the analog data acquisition system to one o
21. ottle 1 1 C pH SET UP MENU Low buffer pH 4 0 High buffer pH 7 0 Temperature comp AUTO Manual temp Value 20 C Type login code to get Access LU C eua Code int vao 51761 88 688 88 18 18 APPENDIX 1 SYSTEM SPECIFICATIONS Technical specifications Wire Interface Voltage Current max Dimensions Net weight Environmental humidity Environmental operating temperature Environmental storage temperature External 100 240VAC to 24VDC ADAPTER 10 3 3Amps at 24VDC 34 x 25 x 15 cm LxDxH 5kg 20 to 80 RH none condensing 15 to 40 C 4 to 70 C Technical specifications Confocal Wire Myograph 360CW Vessel size Vessel alignment Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micrometer Force calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Serial output Voltage Ambient temp 260 um Manually X Y amp Z settings Single bath conical shape Acid resistant stainless steel Max 10 ml NO With connections for suction and gassing Yes 100 to 200mN O 1mN Manually operated precision micrometer Manual Built into chamber independent of superfusion Ambient temp 50 C 5 External Force mN Up to four outputs 1 0V full scale for all acquired signals user defined Serial interface RS232 RS485 100 to 240 VAC a
22. s of strong electromagnetic radiation e g unshielded intentional RF sources as these may interfere with the proper operation 4 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL UNPACKING THE WIRE MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during handling and shipping If you Suspect any kind of damage please contact DMT immediately and the matter will be pursued as quickly as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Wire Myograph System Please contact DMT Sales Department for packing instructions if the original packing material is unavailable After unpacking your new Wire Myograph System please use the following list to check that the system is complete Wire Interface Power supply the shape of the AC plug varies by country be sure that the plug has the right shape for your location Confocal Wire Myograph 360CW or Wire Myograph 320A Myograph connection cable with a temperature probe e Plastic mounting jaws 360CW or stainless steel mounting jaws with supports 320A e Chamber cover Accessories 1x calibration kit including bridge T balance and 2 gram weight e 1xfunnel e 1xstainless steel wire 40 um e 2xAllen keys 360CW or 3 x Allen keys 320A 1xhigh vacuum grease e 1x
23. tem is auto initializing while this screen is displayed CHAPTER 3 11 3 3 Main Menu The Main Menu gives a good overall status on how the Wire Interface is working It displays the force pH probe temperature timer and the active status buzzer timer heat Icon for Heater ON OFF Timer M i Icon for Buzzer ON OFF i MAIN MENU 00 00 00 1 f ON the icon is reen Force chamber 20 2 mN Probe temperature 36 8 C DH value 6 81 TIMER ZERO HEAT BUZZER SETTING Buzzer icon This icon indicates the status on the Buzzer If the icon is grey the Buzzer is OFF If the icon is green the Buzzer is ON active and will make a sound when the timer reaches zero OFF ON Timer The Timer is a countdown timer that can be set to maximum 24 hours If the 00 00 00 Buzzer is activated it will buzz when the timer reach zero Heat icon This icon indicates the status of the chamber heat If the icon is grey the Heat is OFF If the icon is green the Heat is ON active OFF ON Four sub menus are accessible from the Main Menu screen Zero Menu Heat Menu Timer Alarm Menu Setting Menu 3 4 Zero Menu Is used to zero the output from the force transducer Pressing ZERO will reset the baseline of the chart traces without affecting the cali
24. uto 50 60Hz 15 t0 30 C Technical specifications Single Wire Myograph 320A Vessel size Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micrometer Force calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Serial output Voltage Ambient temp gt 60 um Single bath Acid resistant stainless steel Max 10 ml No Yes Yes 100 to 200 mN 0 1 mN Manually operated precision micrometer Manual Built into chamber independent of superfusion Ambient temp 50 C External Force mN Up to four outputs 1 0 V full scale for all acquired signals user defined Serial interface RS232 RS485 100 to 240 VAC auto 50 60 Hz via external power supply 15 to 30 C APPENDIX 1 21 Optional accessories Enable pH meter on the Wire Interface range pH O 14 temp correction 5 5 Electronic valve 100 to 240 VAC auto 50 60 Hz via external power supply 22 WIRE MYOGRAPH SYSTEM 360CW 320A USER MANUAL NOTES NOTES 23 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com salesedmt asia com
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