MPW-260/R/RH - MPW MED. INSTRUMENTS Spółdzielnia Pracy
Contents
1. Caution Fitting the rotor too shallow will result in lack of identification of the rotor after start A of the centrifuge displaying the ERROR ROTOR VER message and stopping the centrifuge Screw in the bolt for fixing the rotor clockwise and screw it tightly home with the supplied spanner for the rotor Swing out rotors have to be provided with the buckets in all seats One should remember that every buckets swings individually Bucket suspension studs should be lubricated periodically with technical petroleum jelly In the case of rotors designed with the cover they must not be used without it Rotor covers must be closed exactly Rotor covers ensure smaller drags of the rotors proper setting of the test tubes and airtight sealing One should use only buckets intended for selected types of the rotor see p 2 1 Accessories Fill test tubes outside the centrifuge Put on or screw the caps on vessels and rotors if applicable In the case of centrifuging in an angle rotor test tubes buckets have to be filled properly in order to avoid overflows CAUTION Centrifuge will tolerate small weight differences occurring during loading of rotors However it is recommended to equalize vessels loads as much as possible in order to ensure minimal vibrations during operation When the centrifuge is started with large imbalance the unbalance control system will switch off the drive system and error signal will be transmitted On the monitoring pane2. centrifuge Cat No 10260 10260R 10260RH user manual Cat No 20260 R RH ENG MPW MED INSTRUMENTS 2012 11 09 USER MANUAL Danilo IH 2600 O o E ty o o Qu Or 6 LABORATORY CENTRIFUGE MPW 260 R RH AN Read this before use MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 610 81 07 service fax 48 22 610 55 36 mpw mpw pl www mpw pl Warning sings and hazard icons WARNING Warning of potential injury or health risk DANGER Risk of electric shock with potential for severe injury or death as a consequence DANGER Biohazard with potential for risk to health or death as a consequence DANGER Risk of explosion with potential for severe injury or death as a consequence P P P P Statement of Conformity The following machine is in accordance with the regulations of the EU Directive 98 79 EC and with the harmonized standards PN EN 61010 1 and PN EN 61010 2 020 CONTENTS 1 APPLICATION 2 TECHNICAL SPECIFICATION 2 1 1 BASIC ACCESSORIES DELIVERED WITH EVERY CENTRIFUGE 2 1 2 OPTIONAL ITEMS 2 2 EXPLOITATION ITEMS 3 INSTALLATION 3 1 UNPACKING OF THE CENTRIFUGE 3 2 LOCATION 3 3 CONNECTION TO MAINS 3 4 FUSES 4 CENTRIFUGE OVERVIEW 4 1 OVERVIEW 4 2 CONTROL AND DISPLAY ELEMENTS 5 SAFETY CONDITIONS 5 1 SERVICING PERSONNEL 5 2 GUARANTEE AND OPERATIONAL USE PERIOD 5 3 SAFEKEEPING PERIOD 5 4 H
3. After setting required parameters program is being recorded in the memory by means of PROG MEMO key Confirmation of the program recording is prolonged by sound signal It is possible to resign from the changes introduced at any moment and leave programming mode without recording by pressing STOP key 7 2 9 Cancellation of the programs Centrifuge program enables complete cancellation of the programs being recorded After pressing PROG MEMO and No 9 key clear message is displayed Pressing PROG MEMO keys ereases all programs recorded up till now while use of STOP key causes cancelling this option 7 2 10 Version of the centrifuge Pressing PROG MEMO and No 7 keys will cause the centrifuge and software versions displaying e g 260RH 0844 522 and total time of work of the centrifuge in hours and minutes _268 _ 8544 5 E 3582 17 24 7 3 Mathematical relations 7 3 1 RCF relative centripetal force RCF acceleration is the acceleration generated by the rotary movement of the rotor acting upon tested product and it can be calculated according to the formula 11 18 x r x n 1000 RCF xg r cm n rpm Depending on the distance of particles of the tested product from the axis of rotation one can establish with use of the above formula the minimum average RCF or maximum On the basis of preset RCF value and given radius of the bottom of the bucket one can calculate with it the rotational speed
4. 12193 Swing out rotor 8 x 15 10 ml 4000 2504 140 54 12218 Swing out rotor 2 microtiter plates MTP 3000 916 9 1 7 7 12232 Swing out rotor 4 x 7 x 10 5 ml 4000 2308 129 6 3 12300 Hematocrit rotor 24 capillary tubes 75mm 13000 16816 8 9 1 4 BUCKETS Catalog no Application 13080 Bucket 17 7x87 mm for 15 10 7 6 ml test tubes for rotor 11217 11740 11741 11745 13081 Bucket 17 7x65 for 10 6 5 ml test tubes for rotor 11217 11740 11741 11745 13170 Bucket 13x81 mm for 12x5 ml test tubes for rotor 12232 13182 Bucket 45x89 for 100 ml test tubes and round carriers 14186 14192 14196 for rotor 12183 13184c Bucket 45x96 for 100 ml test tubes and round carriers 14186 14192 14196 for rotor 12183 13195 Hanger for 2 buckets 13080 for 15 ml Falcon test tubes for rotor 12183 13219 Bucket 85x130 mm for 1 microtiter plate for rotor 12218 13233 Bucket 57 2x66 70 ml for rotor 12232 for round carriers 14169 14235 14238 14239 14240 14242 13266 Bucket 30x100 mm for 50 ml Falcon test tubes for rotor 12183 13267 Bucket 30x100 mm for 50 ml Falcon test tubes with thread or cap for rotor 12183 13275 Bucket 30x99 mm for 50 30 25 ml Falcon test tubes with thread or cap for rotor 11213 13276 Bucket 30x96 mm for 50 30 25 ml Falcon test tubes for rotor 11213 11744 11746 13329 Bucket 25 5x86 mm for 30 25 ml test tubes for rotor 11742 11743 13719 Round bucket 45 96 mm for 100 ml test tubes for rotor 11718 ROUND CARRIERS Catal
5. 260 R RH laboratory centrifuge can be operated by laboratory personnel after getting acquainted with Operating Instruction Operating Instruction shall be held all the time near the centrifuge Operating Instruction must be kept always at hand 5 2 Guarantee and operational use period Guarantee period for the MPW 260 R RH centrifuge amounts to minimum 24 months Principles are specified in guarantee certificate The service life of the centrifuge specified by the manufacturer amounts to 10 years After termination of guarantee period it is necessary to carry out yearly technical inspections of the centrifuge Only service personnel authorized by manufacturer may perform the inspections The manufacturer reserves the right to make modifications to produced goods 5 3 Safekeeping period Maximum period of storage of not used centrifuge amounts to 1 year After this period a service authorized by manufacturer should carry out technical inspection of the centrifuge 5 4 Hints on centrifuging Set the centrifuge in horizontal position on rigid base Ensure safe positioning location Ensure free space around the centrifuge amounting to at least 30 cm left free Ensure sufficient ventilation Fix the rotor on the motor axis firmly A e Avoid unbalance A e Load opposite buckets with the same accessories e Centrifugation of the test tubes of different sizes There is a possibility to centrifuge test tubes of diff
6. 5 n 4 11 18 1000 5 200 Drawing No 5 Nomograph 31 14 2 Table of chemical resistance to the interaction of various categories of reagents of plastics Groups of the substance PS SAN PMMA PC PCV POM PE LD PE HD PP PMP ECTFE PTFE FKM EPDM NR SI in temp 20 C TIRE x Aldehydes Cyclic alcohols Esters Ether Ketones Strong or concentrated acids Weak or diluted acids Oxidizing acids or oxidizing substances z 5 cyclic hydrocarbons Ahs Haloid hydrocarbons Alkalis very good chemical resistance Permanent action of the substance does not cause damage through 30 days The material 1s able to be resistant through years o chemical resistance of good to limited Continuous action of the substance causes insignificant and partly reversible damage through the period of 7 30 days e g puffing up softening reduced mechanical durability discolouring limited chemical resistance The material should not have the continuous contact with the substance The immediate occurrence of damage is possible e g the loss of mechanical durability deformation discolouring bursting dissolving Abbrevia
7. STOP Oo ww MPW 260RH Centrifuge MEW Z60RH COVER SHORT START STOP MW oe 17 Control panel comprises following elements 1 Rotor status signalling behind means of arrows on the display arrow upwards accelerating of the rotor arrow directed at the direction of the indicator of the speed the rotor reached the setup speed down arrow slowing down of the rotor Signalling choice of the COOL AFTER option behind means of star on the display Signalling choice of the AUTO COVER option behind means of wheel on the display Function key START 4 Function key STOP 5 Function key COVER 2 Function key SHORT 3 0 key 9 PRECOOLING program with spinning or without spinning Numeric keypad digits 0 9 7 10 Two function key PROG MEMO 8 11 Two function keys 4 6 A 6 Sound signal serves for signalling function recording and determinating of the centrifuge status N 1 Q WN START key can be used for starting centrifugation program with parameters presented on display STOP key serves for Interrupting centrifugation program in any program phase and braking the rotor Interrupting centrifugation setting parameters without saving them clearing the errors COVER key serves for cover opening PROG MEMO key serves for programs scrolling switching to setting mode Saving program scrolling of programs at down k
8. agents generally used in medical care should be used e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F User is responsible for proper disinfections of the centrifuge if some dangerous material was A spilled inside or outside of the centrifuge When doing above operations one must wear safety gloves 27 9 Troubleshooting 9 1 General errors Almost every error can eliminated by switching the centrifuge OFF and then ON After switching the centrifuge ON parameters of the recently implemented program shall be displayed and sound signals comprising four successive tones shall be generated In case of short duration power failure the centrifuge terminates the cycle and displays PROGRAM ERROR code Please find below the most frequent faults and their repair methods e The above was checked and still there is no display Call service active and check sound audible 2 Centrifuge cannot be started e START key pressing does not generate reaction or single tone only Rotor stopping symbol is not displayed yet Wait till rotor stops and displaying the rotor stopping symbol W Cover opening symbol 4 displayed Close cover Square symbol must displayed arrow on the display is blinking Centrifugation cycle in progress press STOP key or wait till cycle ends e Indications are proof for cycle in progress and Switch power supply OFF ON If fault still persists motor does not start
9. call service e After stopping ERROR UNBALANCE message is displayed Unequal rotor load Centrifuge load shall be balanced Inclined centrifuge Centrifuge shall be leveled Faulty drive mechanical damage Call service Centrifuge was displaced during operation Switch ON the centrifuge afresh after opening and closing the cover Check if rotor number in started program is consistent with the number of the rotor installed in the centrifuge rotor status if there are coding magnets inserted e Centrifuge does not recognize the rotor and does not Switch the centrifuge OFF then ON and check stop correctness of loaded program e After stopping ERROR ROTOR VER message is displayed 28 e Rotor stopping symbol not displayed yet after Rotor is still rotating Wait for stopping of the pressing COVER key single tone is audible rotor and the square symbol displaying e Nothing is displayed Check the centrifuge power supply e Rotor stopping symbol is displayed but cover Call serice can not be opened C viia Wait for stopping of the rotor clear the error by e ERROR POWER FAIL message is displayed pressing the STOP key ERROR T SENSOR message is displayed Check whether the sensor is connected to the steering plate correctly e The sensor is connected correctly and the error is Call service still applying 7 Error of the exceeding the temperature in the Remedy chamber E
10. drive printed or opened with compatible windows application 33 printer Components needed to connect printer name quantity pcs cat no thermal printer KAWKA RS232 cable 16595 16593 Actions Connect printer to the source of power according to the instructions enclosed in user manual connection diagram is given below Connect centrifuge with a printer using delivered data transmission cable 16593 Press START button begin centrifuging When centrifuging process Is finished report will be printed Printout example 82 MIN 88 SEC 82 MIN 88 SEC 2 11771 2888 348 28 C 2888 19 08 47 Cac exo o2 di e a x cr gt lt Lem Ch ka ROZRUCHU 0 00 28 Ch ka HAMOWANIA 6 9 88 22 PREDKOS TEMPERRTURR PREDKOSE TEMPERATURA VIRNIK CZAS RCF CZAS CZAS LICZONY OD HACISHIECIA START PROG KONIEC CYKLU WIROWANIA START CYKLU WIROWANIA PARAMETRY OSIAGNIETE DANE PROGRAMU 2008 91 81 MATERIAL OPERATOR RAPORT NR Centrifuging date and time are listed in the report Date and time are set by the manufacturer for the local area n case of printing incorrect date time one should connect printer to the PC and set current date time using delivered application 34 Connection diagram gt USB CONVERTER gt USB cable RS232 cable PRINTER data transmission cable 15 3 2 Information about
11. manual the user shall contact the device manufacturer in order to check whether the cleaning method chosen does not damage the device 8 2 Cleaning accessories In order to ensure safe operation one shall carry out in regular way periodical maintenance of the accessories Manufactured rotors buckets and round carriers have to withstand steady high stresses originating from the field of gravitation Chemical reactions as well as corrosion combination of variable pressure and chemical reactions can cause corrosion or destruction of metals Hard to observe surface cracks increase gradually and weaken material without visible symptoms In the case of observation of surface damage crevice or other change as well as the corrosion the given part rotor bucket etc shall be immediately replaced In order to prevent corrosion one has to clean regularly the rotor with the fastening bolt buckets and round carriers Cleaning of the accessories shall be carried out outside of the centrifuge once every week or still better after each use Then those parts shall be dried using soft fabric or in the chamber drier at ca 50 C Especially prone to the corrosion are parts made of aluminium For cleaning them one should use neutral agent of pH value from 6 to 8 It is forbidden to use alkaline agent of pH above 8 In this way the useful service life of the device is substantially increased and susceptibility to corrosion is diminished Accurate maintenance incr
12. pressing any key on numeric keypad Single time pressing will make them be displayed speed time acceleration characteristic and deceleration characteristic Double pressing will display the set temperature and RCF value After 3 seconds display returns to displaying current measurements of rotational speed and time Single time pressing Double pressing PRG SPEED 22 7 2 8 Programming Programming mode is activated with pressing PROG MEMO key after previous selection of number of the program which we would like to set or edit On the display occurs PARAM EDIT message that means transformation into setting mode Using A key press forward list of parameters subjected to edition as follows rotor rotational speed acceleration time of rotation in minutes symbol which is before 0 is for infinity centrifuging time of rotation in seconds acceleration mode deceleration mode is for selection time counting begins when START key is pressed or is for selection time counting begins when rpm of the rotor rich rotational speed set up in the program automatic opening of the cover after end of centrifuging cooling temperature possibility to cooling the rotation chamber after the end of program 11291914 and so on in round robin algorithm 25 After setting on selected parameters there is possible to change the parameter s values up 6 key down 4 key
13. to be set in the program of centrifuging Selection of the time of sedimentation and the RCF value shall be carried out experimentally for any given product Once every 100 rpm an electronic circuit automatically calculates and displays RCF value In order to program required RCF value one shall use nomograph Drawing No 5 or change the rotational speed matching displayed value to required acceleration value 7 3 2 Nomograph of relationship rotational speed centrifuging radius RCF Drawing No 5 7 3 3 Maximum load In order to avoid overloading of the rotor one shall observe maximum load which is recorded on every rotor Maximum permissible load is reached when all test tubes are filled with the fluid with 1 2 g cm density If density of the centrifuged liquid is higher than 1 2 g cm then test tubes could be filled only partially or one shall limit operation speed of the centrifuge which is being calculated from the formula n perm n max y y specific gravity cm Nmax max rpm 7 4 Temperature in rotational chamber MPW 260R RH Centrifuge is equipped with ecological refrigerating system with temperature control During centrifugation there may be differences in displayed temperature and real temperature of the samples in the rotor It depends on thermal conductivity of the rotor samples and centrifugation time In order to centrifuge preparations of a lowered temperature stored in a refrigerator i
14. 610 55 36 22 610 81 01 i T VRheinland e DIN EN ISO 9001 E mail mpw mpw pl www mpw pl p e DIN EN ISO 13485 000007 No 10 260 03 ENG We MPW Med instruments Sp dzielnia Pracy 46 Boremlowska Street 04 347 Warsaw Poland PN EN 61326 2 6 PN EN 207 PN EN 13612 2006 PN EN 980 2010 PN EN ISO 18113 3 2011 M P W Med instruments SPOEDZIELNIA PRACY 1 w Warszawie STATEMENT OF CONFORMITY Warszawa 15 03 2012 RU tuv co EN Tel 48 22 673 04 08 22 879 70 46 N Management p Pr Fax 48 22 610 55 36 22 610 81 01 i T VRheinland e DIN EN ISO 9001 E mail mpw mpw pl www mpw pl p e DIN EN ISO 13485 000007 No 10 260R 03 ENG We MPW Med instruments Sp dzielnia Pracy 46 Boremlowska Street 04 347 Warsaw Poland PN EN 61326 2 6 PN EN 207 PN EN 13612 2006 PN EN 980 2010 PN EN ISO 18113 32011 M P W Med instruments SPOEDZIELNIA PRACY 1 w Warszawie STATEMENT OF CONFORMITY Warszawa 15 03 2012 RU tuv co Tel 48 22 673 04 08 22 879 70 46 N Management Pr Fax 48 22 610 55 36 22 610 81 01 i T VRheinland e DIN EN ISO 9001 E mail mpw mpw pl www mpw pl p e DIN EN ISO 13485 000007 No 10 260RH 03 ENG We MPW Med instruments Sp dzielnia Pracy 46 Boremlowska Street 04 347 Warsaw Pol
15. 9 14235 Round carrier 056 5 for 12x50 ml test tubes 12 75 mm for bucket 13233 7 14238 14239 14240 14242 14248 14255 14256 TEST TUBES Catalog no 15015 15016 15040 15046 15048 15050 15052 15053 15054 15055 15067 15098 15100 15102 15115 15116 15117 15118 15119 15120 15121 15122 15124 151253 15127 15128 15130 15131 15419 Round carrier 056 5 short for 7x10 ml test tubes 16 7 75 mm for bucket 13233 Round carrier 056 5 short for 7x5 ml test tubes 13 5 75 mm for bucket 13233 Round carrier 056 5 for 9x2 0 1 5 ml test tubes 611x38 5 mm for bucket 13233 Round carrier 056 5 for 12x1 2 ml S Monovette test tubes 99 13 x 66 mm for bucket 13233 Round carrier 029 8 for 30 25 ml test tubes 26x100 mm for bucket 13266 13267 13275 and 13276 Round carrier 25 for 7 ml test tubes 13x100 mm for bucket 13329 Round carrier 25 for 15 10 ml test tubes 17x120 mm for bucket 13329 Specification Polypropylene test tube 2 ml with cap 6 10 8x41 2 mm Polypropylene test tube 1 2 ml S Monovette with cap 6 8 8 13 4x82 3mm Polypropylene test tube 100 ml with cap 6 44 5x103 mm Polypropylene test tube 14 ml with cap 6 16 8x113 mm Polypropylene test tube 15 ml Nalgene 6 16x113 mm Polypropylene test tube 15 ml Falcon 6 17 21x120 mm Polypropylene test tube 50 ml Falcon 30 35x120 mm Polypropylene test tube 10 ml with cap 6 16 19x100 mm Polypropylene test tube 6 ml with
16. FECTIONS OF ROTATIONAL CHAMBER AND ACCESSORIES 27 9 TROUBLESHOOTING 28 9 1 GENERAL ERRORS 28 9 2 WORK SAFETY INSPECTION 29 9 3 INSPECTION PROCEDURES CARRIED OUT BY THE OPERATOR 29 10 GUARANTEE CONDITIONS 30 DISPOSAL SD 12 MANUFACTURER S INFORMATION 30 13 DISTRIBUTOR INFORMATION A 14 APPENDIX 31 14 1 NOMOGRAPH 31 14 2 TABLE OF CHEMICAL RESISTANCE TO THE INTERACTION OF VARIOUS CATEGORIES OF REAGENTS OF PLASTICS 32 14 3 PRINTING CENTRIFUGING USB RS232 PRINTER 33 Annexes Statement of conformity Declaration of decontamination repair Declaration of decontamination return 1 Application The MPW 260 R RH centrifuges are table top laboratory centrifuge for in vitro diagnostic IVD Devices are used for separation samples taken from people s animal s and plant s components of different densities under the influence of the centrifugal force to provide information about their biological state MPW 260 ventilated MPW 260R with cooling MPW 260R with cooling and heating Its construction ensures easy operation safe work and wide range of applications at laboratories engaged in routine medical analyses biochemical research works etc This centrifuge is not biotight and therefore during centrifugation of preparations requiring biotightness one has to use closed and sealed containers and rotors In the centrifuge it is prohibited to centrifuge caustic inflammable and explo
17. INTS ON CENTRIFUGING 5 5 HAZARDS AND PRECAUTIONS 6 OPERATION 6 1 MOUNTING OF THE ROTOR AND ACCESSORIES 6 3 DRIVE 6 4 DATA INPUT AND OUTPUT 6 5 CONTROLS 6 6 SAFETY DEVICES 6 6 1 COVER LOCK 6 6 2 UNBALANCED LOAD CHECKING SYSTEM 6 6 3 ROTOR INSTALLATION AND SOFTWARE COMPATIBILITY VERIFICATION UNIT 6 6 4 COVER OPENING 6 6 5 CHECKING OF EXCESSIVE TEMPERATURE 7 OPERATING CONTROLS AND FUNCTION 7 1 CONTROL PANEL 7 2 SWITCHING THE CENTRIFUGE ON 7 2 1 SELECTION OF THE PROGRAM 7 2 2 START OF THE PROGRAM 7 2 3 ROTOR INSTALLATION AND SOFTWARE COMPATIBILITY VERIFICATION UNIT 7 2 4 ERROR UNBALANCE FAULT 7 2 5 EMERGENCY STOP 10 10 10 10 10 11 11 11 12 12 12 12 12 13 14 14 15 15 15 16 16 16 16 16 17 17 21 21 21 22 22 7 2 6 END OF THE CENTRIFUGING 22 7 2 7 PARAMETERS MONITORING DURING CENTRIFUGING 22 7 2 8 PROGRAMMING 23 7 2 9 CANCELLATION OF THE PROGRAMS 24 7 2 10 VERSION OF THE CENTRIFUGE 24 7 3 MATHEMATICAL RELATIONS 25 7 3 1 RCF RELATIVE CENTRIPETAL FORCE 25 7 3 2 NOMOGRAPH OF RELATIONSHIP ROTATIONAL SPEED CENTRIFUGING RADIUS RCF DRAWING NO 5 25 7 3 3 MAXIMUM LOAD 25 7 4 TEMPERATURE IN ROTATIONAL CHAMBER MPW 260R RA 25 8 CLEANING DISINFECTION MAINTENANCE 26 8 1 CLEANING OF THE CENTRIFUGE 26 8 2 CLEANING ACCESSORIES 26 8 3 LUBRICATION 26 8 4 GLASS TUBE CRACKING 26 8 5 STERILIZATION AND DISIN
18. and id Heated Laboratory ssified to list A or B and not for PN EN ES 2 020 2008 PN EN 61010 2 101 2005 PN EN 61326 2 6 2006 PN EN ISO 14971 2011 PN EN 13612 2006 PN EN 980 2010 PN EN ISO 18113 3 2011 M P W Med instruments SPOEDZIELNIA PRACY 1 w Warszawie STATEMENT OF CONFORMITY Warszawa 15 03 2012 DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer repair 1 Device SAND AN 2 Description of decontamination see user manual 3 Decontamination carried out by RAR qj hh J kRkKIJk lqll Qo nnnm 4 Date and signature intention DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer return 5 Device STYPE ju u aaa hatsu nuw enceinte serial No 6 Description of decontamination see user manual 7 Decontamination carried out by name 8 Date and signature ooneacnicii Cb ku
19. buckets if such are used 8 Control of execution of the guarantee yearly technical inspection of the centrifuge Only the manufacturer specified holders included in the equipment list as well as centrifuge capillaries which diameter length and durability are suitable should be used for spinning in this centrifuge The use of equipment made by other manufacturers should be consulted with the manufacturer of the centrifuge Disinfectants and cleaning agents generally used in medical care should be used in this centrifuge e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 29 10 Guarantee conditions Manufacturer grants to the Buyer the guarantee on conditions specified in the Guarantee Certificate Buyer forfeits the right to guarantee repair when using the device inconsistently with the Operating Instruction provisions when damage results from the User s fault or when the Guarantee Certificate was lost Repairs should be carried out in authorized service workshops granted with the MPW Certificate The centrifuge shall be sent to repair after decontaminating disinfections Information about authorized service workshops could be obtained from the Manufacturer 11 Disposal When you are disposing the device the respective statutory rules must be observed Pursuant to guideline 2002 96 EC WEEE all devices supplied after August 13 2005 may not be disposed as part of domestic waste The device belongs t
20. cap 6 11 5x92 mm Polypropylene test tube 30 ml with cap 6 24 8x100 mm Polycarbonate test tube 85 ml Nalgene with cap 6 37 8x106 mm Capillary tube stoppers Microhematocrite capillary tubes heparinized 1 4x75mm Micro titer plate with cap 85 5x127 mm Glass tube 100 ml q 45x100 mm Glass tube 50 ml 35x100 mm Glass tube 25 ml 25x100 mm Glass tube 10 ml 16x100 mm Glass tube 7 ml 12x100 mm Glass tube 5 ml 12x75 mm Polypropylene test tube 10 ml with stopper 6 17x70 mm Polypropylene PCR test tube 8x0 2 ml 6x21 mm with integrated caps Polypropylene test tube 0 4 ml 6 5 7x46 mm Polypropylene test tube 0 2 ml PCR 6 6x21 mm Polypropylene test tube 0 5 ml with 7 8x30 mm Polypropylene test tube 1 5 ml with cap 6 10 8x39 mm Polypropylene PCR test tube 8x0 2 ml p 6x21 mm Polypropylene PCR test tube 4x0 2 ml p 6x21 mm Polypropylene test tube 5 ml p 12x75 mm OTHER ACCESSORIES Catalog no 16135 16164 16594 16595 17151 17185 Specification Hematocrite reader flat Hematocrite reader round Data recording set of working parameters by USB port Thermal printer of working parameters by serial RS 232 Polycarbonate cap for bucket No 13267 13275 Aluminium cap for bucket No 13184 CAUTION Optional accessories marked by are delivered for individual order 2 2 Exploitation items A For operating in centrifuge on
21. d program and displays program number rotational speed duration of centrifugation and cover opening status Provided that rotor in the centrifuge is stopped and then it is possible to open the cover by means of COVER key 7 2 1 Selection of the program Control panel can save up to 99 programs preset by the user Selection of the program consists in selection of its number by means of numeric keypad as follows for 1 digit number press digits on numeric keypad for 2 digit number press A key symbol will be displayed press A key symbol will be displayed press tens digit digit will be filled in in tens place 4 press units digit digit will be filled in in units place 4 7 Program 0 is reserved for the PRECOOLING function preliminary cooling of the rotation chamber The choice of this function is occurring after pressing the 0 key time with spinning 4 t 916 PRECOOLIWNG two times without spinning BBJ PRG PRECOOLING After selecting program number in relevant fields will be displayed parameters of this program In order to make calling easier were introduced functions increment program number A key and decrement program number PROG MEMO key Those functions are active for 3 seconds from last program selection 7 2 2 Start of the program After selection of program number and checking if proper rotor was installed consistent with requirements of th
22. e has to be consistent with local supply voltage MPW Med A instruments laboratory centrifuges are 1 safety class devices and they are provided with the three core cable with the plug resistant to dynamic loadings Mains socket shall be provided with the safety pin It is recommended to install emergency cut out that shall be located far from the centrifuge near the exit or beyond the room Before switching on check whether the centrifuge is connected to power supply correctly Check centrifuge before usage whether she is installed correctly 3 4 Fuses The centrifuge is equipped with thermal current protection Fuse is situated in the plug in socket unit at back wall of the centrifuge 10 4 Centrifuge overview 4 1 Overview New generation of MPW Med instruments laboratory centrifuges Is provided with state of the art microprocessor control systems very durable and quiet asynchronous brushless motors and accessories consistent with requirements of the present day user 4 2 Control and display elements 1 Power switch 4 2 Control panel 3 3 Cover 4 Inspection glass 2 5 Emergency cover open place 1 Drawing No 1 General view Drawing No 2 Back of centrifuge 1 Plug in socket 2 Fuse base 3 RS 232 socket 1 2 3 1 Motor axle 2 Rotor 3 Rotor cover 4 Complete clamp Drawing No 3 Unit elements of the angle rotor 11 5 Safety conditions 5 1 Servicing personnel The MPW
23. e should use only original company s buckets comprised in the specification of accessories as well as test tubes for centrifuges of proper diameter length and strength Utilization of test tubes of other makes shall be agreed upon with manufacturer of the centrifuge For cleaning and disinfecting one should use agents generally applied in the health service such as e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 3 Installation 3 1 Unpacking of the centrifuge Open the package Take out the cardboard box containing the accessories Take out the centrifuge from the package Keep the package and packing materials at hand for service transport 3 2 Location The centrifuge shall not be located near source of heat and shall not be subjected to direct sunlight A The table for the centrifuge shall be stable and shall have flat levelled table top It is necessary to ensure a safety zone of the minimum 30 cm round the centrifuge from every direction Normal operating conditions ambient temperature is from 15 C to 35 C Passed parameters of the centrifuge are referring to the above named temperatures At the change of the place from cold to warm one condensation of water will occur inside the centrifuge It is important then that sufficient time be provided for drying the centrifuge prior to starting the centrifuge again minimum 4 hours 3 3 Connection to mains Supply voltage given on the rating plat
24. e spinning process shall be stopped with simultaneous displaying the ERROR ROTOR VER message The conformity of the type of the rotor is signalled with a single audible signal 6 6 4 Cover opening Opening of the centrifuge s cover is possible only with the rotor in the state of rest This state is being checked by the microprocessor which recognizes and signals the rest state prior to opening the cover 6 6 5 Checking of excessive temperature If the temperature of rotation chamber exceeds 60 C possible malfunction of cooling system the drive will be switched off and ERROR OVER TEMP message will be displayed The device will reboot after complete cooling down 16 7 Operating controls and function Power switching ON OFF is carried out with master switch situated on right side of the centrifuge All settings on the centrifuge are done by means of the control panel Panel comprises control keys display and signalling LED s 7 1 Control panel The control panel placed on the front casing serves the purpose of controlling centrifuge operation 6 x centrifuge MEW Zb0ICH 200 PRG 3 COVER SHORT START STOP ww 60 1 2 3 4 5 Drawing No 4 Control panel 1 Display 5 STOP key 9 0 key 2 COVER key 6 Function keys 3 SHORT key 7 Numeric keypad 4 START key 8 PROG MEMO key MPW 260R centrifuge 220 Centrifuge 220202 COVER SHORT START STOP COVER SHORT START
25. eases the service life as well and protects against premature rotor failures Corrosion and damages resulting from insufficient maintenance could not be subject of claims lodged against the manufacturer 8 3 Lubrication The rotor pins shall be always lubricated with technical petroleum jelly In this way the uniform deflection of the buckets and quiet centrifuge operation is ensured 8 4 Glass tube cracking In the case of glass tube cracking all debris shall be accurately removed Rubber inserts shall be exactly cleaned or possibly replaced Otherwise one has to take into account following possibilities Glass particles left in the rubber cushion pad will cause once more glass cracking Glass particles left in containers make impossible uniform deflecting of the buckets and round carriers resulting in unbalance Glass particles left in the rotor chamber cause metal abrasion because of strong air circulation This dust will not only contaminate the centrifuge chamber rotor buckets carriers and centrifuged material but will cause as well damages of surfaces of the accessories rotors and the rotation chamber For complete removal of glass particles and metal dust from the rotor chamber it 1s recommended to place strip of vaseline on the bowl from the top down to bottom Then rotor shall operate for several minutes at moderate speed Glass and metal particles will gather on lubricated area and could be easily removed with the piece of clo
26. ecting preliminary cooling with spinning with constant parameters speed 2500 rpm temperature 4 C time to 15 minute this function is recommended by MPW double pressing makes selecting preliminary cooling without spinning with parameters speed 0 rpm temperature 4 C time to 15 minute 15 6 6 Safety devices Apart from the above described passive devices and safety measures there exist as well active devices and elements as follows 6 6 1 Cover lock The centrifuge can be started only when its cover is properly closed the symbol will display the cover can be opened only after stopping the rotor In the case of emergency opening of the cover during operation the centrifuge will be immediately switched off and the rotor will brake till complete stopping When the cover is opened the amp symbol visible the drive is completely disconnected from the source of power what makes it impossible to start centrifugation 6 6 2 Unbalanced load checking system When loads of opposite buckets or carriers in rotors are unbalanced the drive will be switched off during acceleration or operation of the centrifuge and the ERROR UNBALANCE message will be displayed 6 6 3 Rotor installation and software compatibility verification unit Directly after starting centrifuging a unit verifies the type of the rotor applied and in the case of its incompatibility with the type indicated in the application or absence of the rotor th
27. erent sizes however it is absolutely necessary in such cases that opposite buckets and round carriers be the same Mass of different containers with test tubes spun at the same time has to be comparable e Not only the test tubes shall be inserted symmetrically but also round carriers and their hangers shall be equally loaded It is e g not allowed to operate centrifuge only external part of reductive insert loaded 12 A A N 5 5 Hazards It is necessary to insert test tubes symmetrically on the opposite sides Fill test tubes outside the centrifuge Please pay special attention to the quality and proper thickness of the glass test tubes walls Those shall be test tubes for centrifuges of proper durability up to 5 000 x g In order to protect the centrifuge against unbalance fill in the test tubes up to the same weight Lubricate the swing out rotor journal pins Use only accessories in good condition Protect equipment against corrosion using accurate preventive maintenance Infectious materials could be processed in closed buckets only It is not allowed to centrifuge explosive and inflammable materials It is not allowed to centrifuge substances prone to reacting in result of supplying high energy during centrifugation and precautions Prior to switching the centrifuge on one shall read carefully all sections of this instruction in order to ensure smooth operation and avoid damages of this device
28. errors during centrifuging added to the report In case of the interruption of the centrifugation cycle instead of the command END CYCLE OK the command END CYCLE FAIL plus error number and the achieved parameters are printed or displayed on the screen Error types interrupting centrifugation program by pressing STOP button error number FAIL 3 and description STOP interrupting centrifugation program by imbalance sensor switching on error number FAIL 2 and description UNBALANCE 15 3 3 Date and time of centrifuging Actual date and time for the thermal printer is set up by the manufacturer for his local time In case of printing incorrect date and time one should connect printer directly to the PC computer and using installation software program CD delivered with the printer adjust date and time 15 4 Obtaining Centrifugation Parameters Protocol by computer Report can be obtained by connecting centrifuge directly to the computer 15 4 1 Obtaining printouts To print out the report follow the steps set forth bellow Connect the centrifuge directly to the computer serial interface RS 232 with the cable RS 232 provided as accessory of Assembly for recording of working parameters through RS 232 port see Fig 3 Socket is situated at the back of the centrifuge 35 RU tuv co Tel 48 22 673 04 08 22 879 70 46 N Management p Pr Fax 48 22
29. ey serves for scrolling of parameters list when in programming mode programs scrolling introducing of the double digit number of the program SHORT key serves for short duration operation Pressing will increase speed of the rotor to the speed saving in the program The centrifuge will be working as long as the key is pressed The time is being taken into account from pressing of the SHORT key Impressing of the key will make stopping of the rotor 0 key serves for activate PRECOOLING program with spinning or without spinning Yellow key 4 serves for changing down parameter s value Yellow key serves for changing up parameter s value 12 Display field 1 On the top of display are contains the parameters of the centrifuging lower part are displayed messages of the state of work I program number value of speed setup working time closed cover symbol 1 3n PRG SPEEL 18 II symbol of the choice of the AUTO COVER option opened cover symbol values and symbols of acceleration deceleration symbol of the rotor status Dre IV MPW 260R RH symbol of the choice of the COOL AFTER option temperature value 13 ERRORS gt ERROR USER STOP gt ERROR ROTOR VER gt ERROR UNBALANCE 19 gt ERROR T SENSOR gt ERROR POWER FAIL gt ERROR MOTOR FAIL gt ERROR OVER TEMP 20 7 2 Switching the centrifuge on After switching power ON control system calls recently implemente
30. is program it s possible to start centrifugation process by single pressing of START key The centrifuge can be started provided that cover is closed symbol Bison ERROR message is not displayed 7 2 3 Rotor installation and software compatibility verification unit It is impossible to set rotor speed above maximum rotational speed preset for it It is impossible as well to centrifuge without the rotor or with the rotor with the other number that preprogrammed because the system verificated correctness of installed rotor and compare number of the installed rotor with programmed one at low rotational speed range In case of incompatibility centrifuge will be stopped and ERROR ROTOR VER message will be displayed Consecutive start of centrifuge is possible only after cancellation of the ERROR ROTOR VER message pressing the STOP key then introduction of proper speed correction or installing proper rotor with preset number 21 7 2 4 ERROR UNBALANCE fault The centrifuge is provided with the rotor unbalance sensor and when it will be activated centrifugation process will be stopped through fast braking and at the same time ERROR UNBALANCE message will be displayed Cancellation of this error is possible only through pressing COVER key after stopping of the rotor One must check if rotor was correctly loaded close the cover and once more start the program In order to protect the rotor against beating in opposite areas of the rotor it has t
31. ivered with following accessories HS hermetically sealed HS accessories maintenance Make sure that rubber O rings are lightly coated with silicone grease Use high vacuum grease e g type by LUBRINA ANGLE ROTORS Cat No Type of rotor Angle Rotor capacity DN x Tmin rpm em cm 11199 Angle rotor HSL 45 12 2 0 1 5 ml 18000 24088 6 65 3 5 11213 Angle rotor 30 8x 50 ml 5000 3494 12 5 7 9 11216 Angle rotor 30 12x5 ml 14000 19064 8 7 3 5 11217 Angle rotor 30 10x15 ml 6000 4226 10 5 4 0 11461 Angle rotor HSL 45 24 x 2 0 1 5 ml 14000 18187 8 3 5 0 11462 Angle rotor HSL 45 36 x 2 0 1 5 ml 14000 18187 8 3 4 0 11715 Angle rotor HSL 30 10 x 10 ml 14000 15558 7 1 3 0 11716 Angle rotor HSL 45 4x8 x 0 2 ml PRC 14000 15339 70 6 2 11717 Angle rotor HSL 45 18 x 2 0 1 5 ml filter 14000 18187 8 3 5 0 11718 Angle rotor 30 4x 100 ml 5000 3158 11 3 5 0 11740 Angle rotor 30 12x 15 ml 5500 4058 12 4 6 11741 Angle rotor 30 8x15ml 6000 4226 10 5 4 0 11742 Angle rotor 45 4x 30 25 ml 6000 4829 12 55 11743 Angle rotor 30 12 x 30 25 ml 4500 2716 12 5 0 11744 Angle rotor 30 10 x 50 ml 4500 2830 12 5 4 3 11745 Angle rotor 300 24x 15 10 ml 5000 3354 12 4 6 11746 Angle rotor 30 6x 50 ml 6000 4427 Jl 4 9 SWING OUT ROTORS Cat No Type of rotor Rotor capacity oe RCF ju in 12172 Swing out drum rotor 6 adapters 20 x 0 4 0 2 ml 14000 15338 7 0 3 0 or 10 x 2 0 1 5ml 12183 Swing out rotor 4 x 100 ml 4000 2469 13 8 432
32. l ERROR UNBALANCE message will be displayed In order to prolong lifetime of the rotor and gaskets rotors shall be lubricated with the maintenance oil while gaskets and threaded parts shall be lubricated with the technical petroleum jelly For replacement of the rotor one shall unscrew clamping and then grab the rotor with both hands at opposite sides taking it away from drive shaft by pulling it up 14 6 2 Construction and safety measures The centrifuge has rigid self supporting structure Housing was made of sheet aluminium back made of steel sheet Front and cover was made of ABS type plastic Cover 1s fixed on steel axles of hinges and from the front it is locked with two electromagnetic locks blocking possible opening during centrifugation Rotation chamber casing was made of thick steel sheet The rotation chamber bowl is made of stainless steel sheet Rotors and containers are made of aluminium lids are made of polycarbonate and reductive inserts are made of the polypropylene 6 3 Drive Low noise induction motor constitutes the drive 6 4 Data input and output Data setting and read out system forms hermetically closed keyboard with distinctly accessible operation points Easily readable displays signalling individual performed operations facilitate operator s programming and recording of parameters and condition of the centrifuge The centrifuge is equipped with USB interface It enables connection of the centrifuge to ex
33. mergency cover release In case of e g power failure it 1s possible to open the cover manually On the left hand side of the casing there is lock witch it is necessary to turn to the right with the emergency open key 17642 to the unblocking cover moment The cover can be unlocked and opened only when the rotor is in the rest state 9 2 Work safety inspection For safety reasons inspections of the centrifuge carried out by the authorized service at least once a year after the period of warranty The reason for more frequent inspections could be corrosion inducing environment Examinations should end with issuing Report of validation the check on the technical state of the laboratory centrifuge It is being recommended to establish Technical passport or Log of the apparatus in whom every repairs and reviews are being registered Both these documents should be stored in the place of use of the centrifuge 9 3 Inspection procedures carried out by the operator Operator has to pay special attention to the fact that the centrifuge parts of key importance due to safety reasons are not damaged This remark is specifically important as for 1 Motor suspension 2 Motor axis concentricity 3 Fixing the pins in the bucket 4 Centrifuge accessories and especially structural changes corrosion preliminary cracks abrasion of metal parts 5 Screw joints 6 Inspection of the rotor assembly 7 Inspection of bioseals of the rotors and
34. o 8 group medical devices and is categorized in business to business field The icon of the crossed out trash can shows that the device may not be disposed as part of domestic waste The waste disposal guidelines of the individual EC countries might vary If necessary contact your supplier 12 Manufacturer s information MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 610 5667 sales department 48 22 610 81 07 service fax 48 22 610 55 36 e mail mpw mpw pl www mpw pl 13 Distributor information DISTRIBUTOR 30 14 Appendix 14 1 Nomograph Centrifuging radius cm p m R C F x g 50 multiple of 20000 gravitational acceleration 45 100000 15000 40 13000 Formula used for calculation of this 12000 nomogram 50000 11000 2 40000 10000 s R C F 11 18 r n 1000 30000 9000 where B5 20000 ove R C F multiple of 7000 29 gravitational speed 28 Me 27 r centrifuging radius cm 10000 6000 n rotational speed r p m 26 8000 B 25 g gravitational acceleration 5000 24 6000 22 4000 C 4000 21 3000 3500 20 2400 2000 3000 1500 18 1200 2500 A 17 1000 19 ie 2000 1 600 500 400 k 200 1500 13 1400 1300 12 200 1200 Example of making use 150 1100 11 of the nomogram 120 1000 100 900 10 800 60 A 14 4 cm j 7 5 B 4600 r p m 50 dd C 3400 x g i 600 30 25 8 20 500 15 RCF 400 n 1000 m 11 18 r 8 RCF 6 300 2
35. o be provided with identically filled buckets carriers test tubes etc for getting the best balance possible Unbalance causes noise and vibrations during operation and adversely affects power transmission system motor shock absorbers The better balance the smoother will be the centrifuge operation and therefore longer useful life of driving system Moreover ideal separation level is obtained as already separated constituents would not be moved up by vibration 7 2 5 Emergency stop In any centrifuging moment it s possible to interrupt the process and fast stop the rotor with single pressing STOP key The ERROR USER STOP message will be displayed as a result of that the application wasn t ended correctly 7 2 6 End of the centrifuging After ending the time of centrifuging set in the program the rotor decelerates in accordance with chosen characteristics At end of deceleration rotational speed drops slower in order to ensure soft settling of rotor carriers After stopping sound signal is generated symbol and CYCLE FINISH message is displayed clearing this message is occurring after pressing the STOP key or after opening the cover After pressing COVER key the cover opens and symbol is displayed 1 AE 1 89 CYCLE FINISH TEE 7 2 7 Parameters monitoring during centrifuging For easy finding in the course of centrifuging in order to check preset parameters monitoring function was introduced that is called by
36. og no Application 14000 Adapter 20x0 4 ml for rotor 12172 14002 Adapter 10x2 0 1 5 ml for rotor 12172 14024 Round carrier 044 5 for 15 ml Falcon test tubes 0 17x120 mm for bucket 13182 and 13084 14082 Round carrier 17 3 for 7 5 ml test tubes 6 13 3x100 mm for bucket 13080 and 13081 14084 Round carrier 11 0 for 0 5 ml test tubes 8 0x30 mm for rotor 11199 11461 11462 14089 Round carrier 029 for 15 ml Falcon test tubes 0 17x120 mm for bucket 13266 13267 13276 14126 Round carrier 11 for 0 4 ml test tubes 5 8 46 mm for rotor 11259 11461 11462 11739 14133 Round carrier 10 8 for 0 2 ml test tubes 06 2x21 mm for rotor 11259 11461 11462 11739 14169 Round carrier 056 5 for 8x4ml test tubes 13 5x8 for bucket 13233 14181 Round carrier 044 5 for 5x2 7 ml test tubes 4 12 5x58 mm for bucket 13182 13184 14186 Round carrier 044 5 for 4x7 ml test tubes 13 1 100 mm for bucket 13182 13184 14187 Round carrier 044 5 for 4x10 ml test tubes 16 1 100 mm for bucket 13182 13184 14188 Pad under 100 50 30 25 ml test tubes for bucket 13182 13184 13719 14189 Round carrier 044 5 for 50 ml Falcon test tubes 0 30x120 mm for bucket 13182 13184 13719 14190 Round carrier 044 5 for 30 25 ml test tubes 25 5x100 mm for bucket 13182 13184 13719 14192 Round carrier 644 5 for 50 ml test tubes 35x100 mm for bucket 13182 13184 13719 14196 Round carrier under 100 ml test tubes for bucket 13182 13184 1371
37. or its accessories Centrifuge shall not be operated by unqualified personnel Centrifuge must not be transported with the rotor mounted on the shaft One must use original rotors test tubes and spare parts only In the case of faulty operation of the centrifuge one shall ask for assistance of service of MPW Med instruments Company or its authorized representatives It is not allowed to switch the centrifuge on if it is not installed properly or rotor is not fitted correctly The centrifuge must not be operated in places where explosion hazard exists as it is not explosion proof It is not allowed to centrifuge materials capable of generating inflammable or explosive mixtures when subjected to air It is not allowed to subject to centrifugation toxic or infectious materials with damaged leak proof seals of the rotor or test tube Proper disinfection procedures have to be carried out when dangerous substances contaminated the centrifuge or its accessories 13 A e It isn t allowed to open the cover manually in emergency procedure when rotor is still turning e It isn t allowed to exceed load limit set by the manufacturer Rotors are intended for fluids of average homogeneous density equal to 1 2 g cm or smaller when centrifugation is carried out at maximum speed When fluids of higher density shall be used then it is necessary to limit speed see point 7 3 3 Maximum load e It is not allowed to use the roto
38. rs and round carriers with signs of corrosion or other mechanical defects e It is not allowed to centrifuge highly corrosive substances which may cause material impairment and lower mechanical properties of rotor and round carriers e It isn t allowed to use rotors and accessories not admitted by the manufacturer Let to use commercial glass and plastic test tubes which are destined to centrifuging in this laboratory centrifuge One should absolutely not use poor quality elements Cracking of glass vessels and test tubes could result in dangerous vibration of the centrifuge e It is not allowed to carry out centrifugation with the rotor caps taken off or not driven tight e It is not allowed to lift or shift the centrifuge during operation and rest on it It is nor allowed to stay in the safety zone within 30 cm distance around the centrifuge neither leave within this zone some things e g glass vessels e It is not allowed to put any objects on the centrifuge 6 Operation 6 1 Mounting of the rotor and accessories I 11 12 13 Connect the centrifuge to the mains master switch on right side of the centrifuge Open the cover of the centrifuge by pressing the COVER key Prior to putting the rotor in one has to check if rotational chamber is free of impurities e g such as dust glass splinters residues of fluids that must be taken away One shall fit the rotor on the motor shaft driving it home on the cone
39. sive preparations 2 Technical Specification manufacturer MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland mains voltage L1 N PE 10 PERS RCE time 0 s 99 min 59 s res 1s time counting since start button is pressed since preselected speed is reached short time operation mode SHORT yes up to 15 min continuous operation mode HOLD userprogamms PO adjustable temperature 20 40 C 20 55 C initial cooling PRECOOLING nO 2500 RPM cL 0 RPM 4 C cooling after centrifuging COOL AFTER adjustable curves acceleration deceleration USB interface Z s a EN 5501 ambient conditions PN EN 61010 1 pkt 1 4 1 set up site indoors only ambient temperature 5 40 C humidity maximum relative humidity lt 80 excess voltage category PN EN 61010 1 pollution degree PN EN 61010 1 300 mm 56 dB 46 5 kg 2 1 Accessories 2 1 1 Basic accessories delivered with every centrifuge Cat No Type of accessories pcs 17142 Complete clamp 1 17099T Spanner for the rotor 1 17642 Spanner for emergency opening of the cover 1 17861 Fuses WTA T 4 250 V 2 or 17862 Fuses WTA T 6 3 A 250 V 2 or 17863 Fuses WTA T 10 A 250 V 2 17866 Power cord 230 V 2 or 17866 Power cord 115 V 1 20260 R RH ENG Operating Instruction 2 1 2 Optional items Depending on customer s needs MPW 260 R RH centrifuge can be del
40. t is necessary to make initial refrigerating of the chamber the rotor and containers In order to obtain lower temperatures of the chamber we recommend to follow PRECOOLING operations with centrifuging load 0 program In centrifuge the spin rotors with maximum speed 2500 rpm in 15 min obtaining 4 C temperature without centrifuging load the program pressing 2 times the 0 key Chamber will be cooled down to 4 C temperature without spinning Cooling of the rotation chamber is also occurring when centrifuging in the SHORT function The cooling system allows to cool every rotor with its equipment with maximum speed down to 4 C Obtaining lower temperatures depends on deration the type of rotor and speed 25 8 Cleaning disinfection maintenance CAUTION It is necessary to wear protective gloves when following below instructions 8 1 Cleaning of the centrifuge A centrifuge should be cleaned with water mixed with soap or other water soluble mild detergents One should avoid corrosive and aggressive substances It is prohibited to use alkaline solutions inflammable solvents or agents containing abrasive particles Using wiping cloth remove condensate or residues of the products from the rotor chamber It is recommended to keep the cover opened when the centrifuge does not work in order to expel the moisture In the case the user decides to use centrifuge and equipment cleaning methods other than the ones described in this
41. ternal PC unit with the printer and recording the centrifugation parameters MPW Editor 6 5 Controls The microprocessor control unit of the centrifuge ensures broad possibilities of providing realisation and reading of work parameters that is selection of the spin program 1 99 gt selection of the rotor according to the accession number gt selection of rotational speed within 100 18000 rpm gt setting centrifugation time within 00 00 99 59 min gt selection of SHORT short duration operation gt selecting of the AUTO COVER function automatic opening of the cover after centrifuging is finished choice is signalling by the circle between SPEED and TIME inscriptions gt counting the time of the START key pressing or of the set speed reached Selecting acceleration characteristics from 0 quickest to 9 slowest gt selecting deceleration characteristics from 0 quickest to 8 slowest deceleration characteristic number 9 is subterfuge characteristics with time of free stopping of the rotor setting temperature range 20 40 C setting temperature range 20 55 C BM selection of the COOL AFTER option cooling the rotation chamber after the end of centrifuging choice is signalling by the star between SPEED and TIME inscriptions Ee sls selection of the PRECOOLING program behind means 0 key MY AWPL single time pressing makes sel
42. th together with the grease This operation can be repeated in case of a need 26 8 5 Sterilization and disinfections of rotational chamber and accessories One can use all standard disinfectants The centrifuges and accessories are consists of various materials and one should to take into account possible variety of materials During sterilization by means of steam one should to consider temperature resistance of individual materials STERILIZATION Sterilization Radiation B y Gas Chemical compounds temp 121 C 25 kGy ethylene oxide formalin ethanol yes yes yes yes yes Laboratory vessels must be exactly cleaned and rinsed with the distilled water before the sterilization in the autoclave It is always necessary to remove closures from containers 1 The frequent steam sterilization reduces mechanical durability PC test tubes may become useless 2 Except PVC hoses which are resistant to the steam sterilization in the temperature 121 C Abbreviations of names of characterized plastics High density polyethylene PP Polypropylene S Silicon rubber PMP Polymethylpentene FP For centrifuging infectious materials it is necessary to use hermetically closed buckets in order to prevent they migration into the centrifuge Rotors buckets and round carriers can be sterilized in autoclave with 121 124 C temperature and pressure of 215 kPa during 20 min In the centrifuge disinfectants and cleaning
43. tions of names of characterized plastics PS Polystyrene ECTFE Ethylene chlorotrifluoroethylene SAN Styrene acrylonitrile ETFE Ethylene tetrafluoroethylene PMMA Polymethyl methacrylate PTFE Polytetrafluoroethylene PC Polycarbonate FEP Tetrafluoroethylene perfluoropropylene PVC Polyvinyl chloride PFA Tetrafluoroethylene perfluoroalkylvinylether POM Acetal polyoxymethylenel FKM Fluorcarbon rubber PE LD Low density polyethylene EPDM Ethylene propylene diene PE HD High density polyethylene NR Natural rubber PP Polypropylene SI Silicon rubber PMP Polymethylpentene 22 14 3 Printing centrifuging USB Rs232 Printer When the centrifuging process is finished there is a possibility to obtain report Report can be transferred to PC or printed with a KAWKA printer Devices that offer printing reports are available optionally and they are not delivered with every centrifuge gt PC USB quantity pcs name Connect centrifuge to the PC with the RS232 cable and USB converter connection diagram is given below Install USB converter drivers see attached CD Ensure that serial port COM settings are set as described below 9600 8 n 1 Baud rate 9600 Parity none Stop bit 1 Data length 8 Install MPW Editor application Windows delivered on CD Centrifuging and report printing Begin centrifuging process Complete report is displayed on the PC screen and can be saved on a hard
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