Eppendorf ThermoMixer C Operations Manual (PDF
Contents
1. 9 SBF te 4 se d 3 131 111 1 11111 11 11 ane ails 9 3 3 Information on product ab 9 3 4 Warnings for intended use 9 3 5 Warning signs on the device lslseseleee nennen 12 4 Installation s s e bh Ee 13 4 1 Selecting the location oec Rr E eee eee kage Lowa ER che 13 4 2 Installing the instrument 2 0 0 cece ee 13 5 Operation eoe 2 EN 711111111 e 14 5 1 Overview of operating controls llis 14 5 2 Setting the language e e 16 5 3 Installing the thermoblock 16 5 3 1 Attaching the thermoblock 18 5 3 2 Removing the hermobiock eh 18 5 4 Inserting tubes and plates 2 4 2 4 445645 0 18 5 5 Installing the ThermoTop xe e x e x e e cece x e K III 20 5 6 Temperature control ENEE km Rh hber a 21 5 6 1 Temperature control with time setting llle 21 MM Iro ni 3 3 33 3131 1 1 0 22 5 7 1 Mixing without temperature contre 22 5 7 2 Mixing and tempering IR 22 5 7 3 Mixing temperature control with continuous run 23 5 7 4 Interrupting the mixing process eR 24 5 7 5 Short MiX o sis 3 TRIPS n pbi nete 200 24 5 7 6 Mixing tempering with Time Control or Temp Control 24 Operating manual Eppendorf ThermoMixer C Operating manual 10 5 83 MENU 22 2 ee teur eine net Yu teen Bde Cea Manas paws wees 25 5 8 1 Navigating2. rn re nens Time mode Temp Control 15 24 Time Control s Time setting Application parameters 40 R TTT 16 18 U USB interface unse 7 V Verification of temperature control 36 W Warning sign 1 T 1 0 12 UI 6 45 jenuew Burje1ado Operating manual 46 Eppendorf ThermoMixer C Operating manual P Program data form program time temp speed 1 o gt WIN a A m A C5 gt oa o ai N ak wk D eo rni cl d EG i e Go en onformity D on A E ck nag eclaration d baren Demi at Par dra A grupo E rr ieie ck cer aR Uer dag og ee ger x re kara De r e rah Fe TH pE deaan SE i pn ce wer ck amp s d ar t E Se Ire Grott cina fen Vit Ee aa element be Ea come of une ore erudiri Eh Bc Lae iw Tei voeta Ha vraa vi GX ch ck ct n ca K i LK K g MX KA FT ch cA Sy 7 rm KK AWA YD st TH TR ce LE ch d e 4 Ze ce e e e ET mH A E Opera Derating Manu j Evaluate your operating manual www eppendorf com manualfeedback eppendorf Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendor
3. 1 2 1 Danger symbols N Biohazard A A Explosion A Electric shock Hot surface A Hazard point Risk of fire Y IN L9N d Crushing A Material damage 1 2 2 Danger levels DANGER Will lead to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage jenuew Burje1ado Eppendorf ThermoMixer C Operating manual EN 1 3 Symbols used Symbol Meaning Ki gt Handling gt 5 1 Actions in the specified order E 2 2 5 List D 4 Text Name of fields in the software o i Useful information 1 4 Abbreviations used PCR Polymerase chain reaction rpm Revolutions per minute in rpm 1 5 Glossary Deepwell plate Plate with 48 96 or 384 wells with a larger volume than Pirin microplates Suitable for the preparation mixing centrifuging transporting and storing of solid and liquid samples Lid Lid for the thermoblock Ensures uniform temperature control and protects samples from unwanted exposure to light Microplate Plate with 24 48 96 or 384 wells for the preparation EBENE mixing centrifuging transporting and storing of solid E and liquid samples PCR plate Plate with 96 or 384 wells for PCR applications ThermoTop Heated cover for the thermoblock Prevents the formation of condensation on the inner wall or the lid of the
4. Interval Mix Mixing time 1 00 min Pause 0 30 min In the program level the device performs the setting 1 min mixing and 0 30 min pause 4 times 29 Operating manual 30 Eppendorf ThermoMixer C Operating manual 5 9 3 Restricting ramp rates You can use the ThermoMixer C to choke the heating rate as well as the cooling rate Restricted ramp rates can only be specified for programs Tab 1 Heating rates and cooling rates Heating rate max 3 0 C min max 2 0 C min max 1 0 C min max 0 1 C min Cooling rate max 1 0 C min max 0 5 C min max 0 1 C min 1 Select an empty program space under Menu gt Programs 2 Set the mixing time temperature and mixing frequency with the time temp and speed arrow keys Select Options Confirm with the menu enter key 4 Select Ramp rates Confirm with the menu enter key e P OE ptions Ramp Rates Back Heating rate dar 1 0 min bk Cooling rate mamnimal 5 Use the menu arrow keys to select and change Heating rate or Cooling rate 6 In order to leave the Hamp rates menu select the Back menu item Confirm with the menu enter key When you start a program that runs with restricted heating or cooling rates a message is displayed The program s ramp rates are restricted 5 9 4 Quick save with program keys To quickly save a single level program you can use the program keys 1 Set
5. Eppendorf ThermoMixer C Operating manual eppendorf Copyright 2013 Eppendorf AG Hamburg Germany No part of this publication may be reproduced without the prior permission of the copyright owner eppendorf Eppendorf Thermomixer and Eppendorf ThermoTop are registered trademarks of Eppendorf AG Hamburg Germany condens protect is a trademark of Eppendorf AG Hamburg Germany Trademarks are not marked in all cases with or 9 in this manual Operating nex ER 55 3 Program data form 3 3 1509 223 5382 900 016 01 012013 Eppendorf ThermoMixer C Operating manual EN L Table of contents 1 Operating instructions cr m nr 5 1x1 ne ene heat Oeo eer b OR deo Luke 5 1 2 Danger symbols and danger levels eeee es 5 3 t21 Danger symbols 54 eere icai rn raus base 5 S 1 2 2 Danger leVels TTT 5 EZ 1 3 Symbols Used ag 1 baee ace m usto donatus a cda atus A 6 3 1 4 Abbreviations used cs rR a ga R r r RR 0 R e hh heh rn 6 1 5 Glossa 1 1 50 6 CH 2 Product description a NV KN R 69 6 gR IEN NEE Be ERR RRR e 7 2 Ma inill str tion 25 5 8 ecce ies 7 2 2 Delivery package ies cae mn b hc yk roe HER OR RERO Red 7 2 3 Features et eh Re 3 3 11 1 90 8 9 7 77 7 7 9 3 1 Intended use 2 3 od
6. temp and speed arrow keys 2 Use the menu arrow keys to select Save Confirm with the menu enter key Entering the program name 3 Select letters or numbers with the menu arrow keys and confirm with the menu enter key The program name can have a maximum of 15 characters In order to delete individual characters select lt and press the menu enter key 4 order to save the program with the program name use the menu arrow keys to select Save 5 Select the program space with the menu arrow keys Confirm with the menu enter key Creating a multi level program 1 Select an empty program space in the Menu gt Programs menu item Defining step 1 2 Set the mixing time temperature and mixing frequency for the first program step with the time temp and speed arrow keys Adding step 2 3 Select Options Confirm with the menu enter key P OE Options Back FES S Delete ste Interval Mix Ramp rates 4 Select Add step Confirm with the menu enter key P D Cancel Save ET Step 1 01h D m 2 1000 rpm Step 2 10m 00s 500 rpm E The set parameters have been adopted in step 1 Eppendorf ThermoMixer C Operating manual 5 Set the parameters for the second program level In order to save the program with 2 program levels select Save n order to program a third and a fourth program level respectively select Options gt Add step i In order to delet
7. vibrations of the work surface and e g fall off the work table K Do not place easily movable items near the device or secure them adequately NOTICE Damage to the display due to mechanical pressure K Do not apply mechanical pressure to the display NOTICE Damage to electronic components due to condensation Condensate can form in the device after it has been moved from a cool environment to a warmer environment K After installing the device wait at least for 3 h Only then connect the device to the mains NOTICE Damage from the use of aggressive chemicals K Do not use any aggressive chemicals on the device or its accessories such as Strong and weak bases strong acids acetone formaldehyde halogenated hydrocarbons or phenol K Ifthe device has been contaminated by aggressive chemicals immediately clean it by means of a mild cleaning agent 3 5 Warning signs on the device Representation Meaning Location Risk of burns from hot surfaces Upper device side On the thermoblock K Observe the operating manual Rear of the device Eppendorf ThermoMixer C Operating manual 4 Installation 4 1 Selecting the location Select the location for the device according to the following criteria Suitable power connection as per the name plate Minimum distance to other devices and walls 10 cm 3 9 in Bench with a horizontal and even work surface which is designed to support
8. 951010057 PCR Tubes 0 5 mL 500 pieces colorless thin walled with hinged lid 0030 124 332 0030 124 200 951010006 PCR Tubes 0 2 mL 1 000 pieces PCR clean colorless clear 0030 124 340 951010014 PCR Tube Strips 0 2 mL five tube strip pack of 125 625 tubes colorless 0030 124 359 951010022 PCR Tube Strips 0 2 mL 10 x 12 strips colorless 0030 128 648 951020401 twin tec PCR Plate 96 skirted Wells colorless 25 pieces low profile border clear 0030 128 575 951020303 twin tec PCR Plate 96 semi skirted Wells colorless 25 pieces standard profile border clear Eppendorf ThermoMixer C Operating manual Order no international Order No North America Description 0030 521 102 951031003 Eppendorf Deepwell Plate 384 200 uL 40 plates wells clear PCR Clean white border color 0030 501 101 951031801 Eppendorf Deepwell Plate 96 500 uL 40 plates wells clear PCR Clean white border color 0030 501 209 951032603 Eppendorf Deepwell Plate 96 1000 uL 20 plates wells clear PCR Clean white border color 0030 501 306 951033405 Eppendorf Deepwell Plate 96 2000 uL 20 plates wells clear PCR Clean white border color All plates are available with different border colors red yellow green and blue and purity qualities in large packs as well as with barcoding on request You can find further informa
9. frequency 14 22 Setting the mixing time SIOP EE Symbol Sep temperature control run 14 with continuous run 23 without temperature control 22 0 Operating controls uuss 7 14 P PAUSG 77 0 24 29 PGR uenenum 6 PCR pl al cintas 6 16 18 Power connection socket 7 7 Program GREGG vantsstaet Multi level Program name 22222224660oii 15 28 Program number 15 SAVE arisini Single level Programi keys ett nennen Ramp rates Restrict oC S Safety Drecautlons sss 9 Select location eee 13 Set Temperature 15 Short MIX tein nint 14 24 Speaker Set the volume eee eee 26 Switch off SYMBOL Eppendorf ThermoMixer C Operating manual Ste iis 77 0 0 7 777 28 Storage 2 3 nadanoandapad 37 T Technical data Ambient conditions 38 Application parameters 39 Power supply 2 nnn 38 Weights dimensions 38 Temperature control Setting the temperature control duration21 Tempering Application parameters 39 Set temperature 14 Switching off the temperature control 22 SYMibol iain Thermoblock Attach Ke dE Thermolop
10. in the menu eos 25 5 82 Menu Str ctUre ue ox rr hA ORE Y ERE E ERO ER den 25 5 9 Proglas rerne 233 3 1313 1311 1131 1 1 1 6 27 5 9 1 Creating a program 27 5 9 2 Interval Mix Creating a program level with pause interval 29 5 9 3 Restricting ramp rates x e e x K e x K x K K KKK 30 5 9 4 Quick save with program Keya ess 30 5 9 5 Loading a saved program 1 eee 31 5 9 6 Editing programs cer re Rm nee he yh EE 32 5 9 7 Deleting programs NEEN t RR REIR E EUER IR 32 Troubleshooting 4 2 esser esa ree EE ri rE Era RRR R qa ERR R SENE 33 6 1 General errors periere teie ARR KR an EAE x RO 33 6 2 Errormessages eid eas 34 Maintenance 3 1 8 90 0 34 7 1 Setting service intervals 0 0 eee 34 7 2 Cleanings bb dee res 35 7 2 1 Cleaning the thermoblock ee 35 7 3 DisinfectionDecontamination I 36 7 4 Decontamination before shipment 2 eese 36 7 5 Verification of temperature Control 36 Transport storage and disposal 2 37 8 1 Transport 1 1 33310 0 0 37 Ge ern ee ne ee 37 8 3 DispOSal na ner a on 37 Technical data 2 7 Y PR EG ER RO 38 9 1 Powersupply 2022 RR ana ae 38 9 2 W
11. keys The 5 most common mixing and temperature control parameters are already Stored as programs and can be selected directly with the program keys The programs can be overwritten Lid and ThermoTop The Lid ensures uniform temperature control and protects samples from unwanted exposure to light The ThermoTop prevents the formation of condensation on the inner wall or the lid of the tube thanks to the condens protect technology Eppendorf ThermoMixer C Operating manual E 3 Safety 3 4 Intended use The ThermoMixer C is intended for use in a molecular biology laboratory The ThermoMixer C is designed for the temperature control and mixing of liquids in closed tubes and closed plates for the preparation and processing of samples The ThermoMixer C is exclusively intended for use indoors All country specific safety requirements for operating electrical equipment in the laboratory must be observed Only use Eppendorf accessories or accessories recommended by Eppendorf 3 2 User profile The device and accessories may only be operated by trained and skilled personnel Before using the device read the operating manual carefully and familiarize yourself with the device s mode of operation 3 3 Information on product liability In the following cases the designated protection of the device may be compromised Liability for any resulting property damage or personal injury is then transferred to the operator The devic
12. of samples after inserting samples into a pre heated thermoblock the displayed actual temperature may fall temporarily While the device is tempering the blue LED of the ThermoTop is flashing Eppendorf ThermoMixer C Operating manual EN 5 6 Temperature control NOTICE Damage to electronic components due to condensation Condensate can form in the device after it has been moved from a cool environment to o a warmer environment 3 K After installing the device wait at least for 3 h Only then connect the device to the s mains Si LO The ThermoMixer C can be used for temperature control in a range of 15 C below the ambient 3 temperature to 100 C S c B When the actual temperature flashes on the display the device is not in temperature control mode operation As soon as the set temperature is changed with the temp arrow keys the device begins to perform temperature control When the set temperature has been reached the display only shows one value 5 6 1 Temperature control with time setting Prerequisites The time mode is set to Time Control see p 24 1 To switch off the mixing function use the speed arrow keys to select the 0 rpm setting v below 300 rpm or A above 3 000 rpm d y 10 0 0 E min rpm 2 Using the time arrow keys set the temperature control duration Using the temp arrow keys set the temperature 4 To start
13. the parameters For programs with program levels Use the or menu arrow keys to select a step change the parameters of the step 3 Start the program After completion of the program a message appears stating that the program has been changed You can confirm or discard the changes 5 9 7 Deleting programs Programs 1 to 5 cannot be deleted You can change and overwrite the program name and all parameters of these programs 1 In order to delete a program from the program spaces 6 to 20 select the program under Menu Programs Confirm with the menu enter key 2 Use the menu arrow keys to select the Delete menu item Confirm with the menu enter key The display shows the message Confirm delete In order to confirm press the menu enter key Eppendorf ThermoMixer C Operating manual C 6 Troubleshooting If you cannot remedy an error with the recommended measures please contact your local Eppendorf partner The contact address can be found online at www eppendorf com worldwide 6 1 General errors Symptom Cause Remedy message Display remains No mains connection K Check the mains connection dark gt and the power supply Switch on device Set temperature is not reached Set temperature is more than 15 C below ambient temperature gt Set up the device in a cooler environment ThermoTop LED does not light No thermoblock has been attached The th
14. the weight of the device Surrounding area must be well ventilated Location must be protected against direct sunlight The mains power switch and cutting unit of the mains power line must be easily accessible during operation e g residual current circuit breaker 4 2 Installing the instrument WARNING Risk from incorrect supply voltage K Only connect the device to voltage sources which correspond to the electrical requirements on the name plate Only use sockets with a protective earth PE conductor and suitable power cable 1 Place the ThermoMixer C onto a suitable work surface Position the device in such a way that the ventilation slots on the underside of the device are not obstructed 2 Connectthe power cable to the power connection socket of the device and the power supply 13 jenuew Burje1ado EN Operating manual 14 Eppendorf ThermoMixer C Operating manual 5 Operation 5 1 Overview of operating controls 1 2 M b prog 2 prog 3 prog 4 rv time temp A T 9 8 7 6 5 4 3 Fig 2 Operating controlsThermoMixer C 1 Program keys with control LEDs Press the program key Load program Keep the program key pressed for 2 s Save current parameters short key Short Mix runs while the short key is being pressed see p 24 3 start stop key Arrow keys speed Press the start sto
15. to infectious liquids and pathogenic germs K When handling infectious liquids and pathogenic germs observe the national regulations the biological security level of your laboratory the material safety data sheets and the manufacturer s application notes K Wear personal protective equipment K For comprehensive regulations about handling germs or biological material of the risk group Il or higher please refer to the Laboratory Biosafety Manual source World Health Organisation Laboratory Biosafety Manual in its respectively current valid version WARNING Risk of fire K Do not use this device to process any highly flammable liquids Eppendorf ThermoMixer C Operating manual WARNING Damage to health due to contaminated device and accessories K Decontaminate the device and the accessories before storage and shipping WARNING Risk of injury due to incorrect consumables Poorly fitting tubes or plates can become detached from the thermoblock Glass tubes can smash K Only use the thermoblocks with the consumables designed for them K Never use tubes made of glass or other fragile material WARNING Contamination due to opening seals of consumables In the following cases the seals of tubes can spring open Sample material can escape high vapor pressure of the content improperly sealed cover damaged sealing lip improperly fastened foil Always check that consumables have been sealed t
16. tube thanks to the condens protect technology Well Cavity Microplate PCR or deepwell plate tube Eppendorf ThermoMixer C Operating manual 2 Product description 2 1 Main illustration Fig 1 ThermoMixer C 1 Operating controls 2 Display Heating cooling plate 4 Centering pins 6 Power connection socket only 3 5 Power switch 7 USB interface for Eppendorf Service 2 2 Delivery package Quantity Order no Order No Description international North America 1 5382 000 015 5382000023 ThermoMixer C basic device without thermoblock 1 Power supply device 1 5382 900 016 Operating Manual ThermoMixer C 1 5382 900 024 Short Instructions ThermoMixer C 0 Check the delivery for completeness Check all parts for damage in transit To safely transport and store the device keep the transport box and packing material jenuew Burje1ado Operating manual Eppendorf ThermoMixer C Operating manual 2 3 Features With the ThermoMixer C two basic sample preparation applications can be easily performed in a single step simultaneous mixing and temperature control of the sample material The thermoblocks can be exchanged quickly and easily without the use of tools The thermoblocks allow the use of lab tubes in the microliter and milliliter ranges Tubes e g Eppendorf Safe Lock Tubes with volumes of 0 2 mL to 5 0
17. 2 Use the menu arrow keys to select the Load menu item Confirm with the menu enter key The display shows the parameters of the program Programs with several program levels The display shows the parameters of the first program level To display the parameters of the other program levels use the 4 or menu arrow keys to select the corresponding step 3 In order to start the program press the start stop key P OB RT eR diu Step METENDp Step Step 01 00 30 12 S00 h min T rpm The asterisk marks the active program level Step 1 The display shows the parameters of Step 2 31 jenuew Burje1ado Operating manual 32 Eppendorf ThermoMixer C Operating manual 5 9 6 Editing programs There are two ways to change a saved program Changing the program via the Edit menu item in the program list Changing the program during operation Changing the program via the Edit menu item in the program list 1 In order to change the parameters of a program select the program under Menu Programs Confirm with the menu enter key 2 Use the menu arrow keys to select the Edit menu item Confirm with the menu enter key The display shows the saved parameters You can change all parameters and save them see Creating a program on p 27 Changing the program during operation 1 Load the program with the program keys or from the program list 2 Change
18. Block 1 5 mL 0 5 C 1 0 C You can find information on the temperature accuracy of other SmartBlocks on the website www eppendorf com Temperature homogeneity In the range of 20 C 45 C max 0 5 C for all positions of the thermoblock Heating rate 6 0 C min The change of temperature in filled tubes is slower Cooling rate if the set temperature is above if the set temperature is ambient temperature between ambient temperature and 15 C below ambient temperature 2 5 C min 0 5 C min 0 8 C min Heating rate and cooling rate can be throttled see Restricting ramp rates on p 30 39 jenuew Burje1ado Eppendorf ThermoMixer C Operating manual EN 9 4 2 Mixing Thermoblock Mixing frequency can be set in increments of 50 rpm E SmartBlock 0 5 mL 300 rpm 2 000 rpm a SmartBlock 1 5 mL 300 rpm 2 000 rpm E SmartBlock 2 0 mL 300 rpm 2 000 rpm amp SmartBlock 5 0 mL 300 rpm 1 000 rpm SmartBlock 12 mm 300 rpm 2 000 rpm SmartBlock cryo 300 rpm 2 000 rpm SmartBlock 15 mL 300 rpm 1 000 rpm SmartBlock 50 mL 300 rpm 1 000 rpm SmartBlock plates with deepwell plates and temperature control up to 80 C with deepwell plates and temperature control from 80 C 300 rpm 3 000 rpm 300 rpm 2 000 rpm 300 rpm 1000 rpm 40 SmartBlock PCR 96 300 rpm 2 000 rpm SmartBlock PCR 384 300 rpm 3 000 rpm 9 4 3 Tim
19. and mixing process begin immediately Time Control Time counting and mixing process begin when the set temperature has been reached Temp Control 1 Under Menu select the Time Mode menu item HMenu Time Mode zm Back Time Control A 2 Use the menu arrow keys to select Time Control or Temp Control A tick indicates the selected setting 3 To exit the menu press the left 4 menu arrow key twice Eppendorf ThermoMixer C Operating manual 5 8 Menu 5 8 1 Navigating in the menu The menu has 3 levels To change settings proceed as follows 1 To open the menu press the menu enter key Select the menu item with the menu arrow keys To confirm your selection press the menu enter key Change the settings with the menu arrow keys 000 89006 To confirm the changed setting press the menu enter key A tick appears in front of the setting 6 To exit the menu level select the Back menu item and press the menu enter key 5 8 2 Menu structure Menu items and options Description Symbol on the display Programs Load saved program Create program List with 20 program spaces Select a program key prog 1 to prog 5 or Select the program from the program list Menu Programs Load Start the program with the start stop key Save the set parameters mixing time tempera
20. aning or disinfection work Do not allow any liquids to penetrate the inside of the housing Use closed tubes and closed plates Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside vv v v Auxiliary equipment Lint free cloth Disinfectant Switch the ThermoMixer C off and isolate from the power supply Allow the device to cool down Clean the device see Cleaning on p 35 Select a disinfection method which complies with the legal requirements and regulations applicable to your range of application 5 Wipe the surfaces with the lint free cloth and disinfectant B Up MIS 7 4 Decontamination before shipment If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following WARNING Risk to health from contaminated device 1 Observe the information in the decontamination certificate You find it as a PDF file on our website www eppendorf com decontamination 2 Decontaminate all the parts you would like to dispatch 3 Include the fully completed decontamination certificate in the package 7 5 Verification of temperature control To verify the temperature accuracy of the thermoblock use the Eppendorf Temperature Verification System Single Channel In combination with the temperature sensor for the ThermoMixer C the exact temperature in the
21. e a step from a program select Options Delete step 5 9 2 Interval Mix Creating a program level with pause interval By using the Interval Mix function you can determine the mixing process to be interrupted by one or several pauses within one program level Interval Mix can only be specified in programs For program levels with Interval Mix the i symbol will appear in the display jenuew Burje1ado 1 Select an empty program space under Menu gt Programs 2 Setthe mixing time temperature and mixing frequency with the time temp and speed arrow keys Select the mixing time so that it covers the entire duration including mixing processes and pauses Select Options Confirm with the menu enter key 4 Select Interval Mix Confirm with the menu enter key e POG ptians Interual Mis Cancel 10 m Mixing time E Oim 00s Pause 5 Set the mixing time before the pause in the Mixing time row using the time arrow keys 6 Set the pause duration in the Pause row using the time arrow keys 7 In order to save the program select Save Confirm with the menu enter key The Interval Mix settings are now saved for the program level 0 To program a change between several mixing processes and pauses within a program level select a correspondingly longer mixing time for the program level Change between mixing process and pause Mixing time set for program level 6 00 min
22. e is not used in accordance with the operating manual The device is used outside of its intended use The device is used with accessories or consumables which are not recommended by Eppendorf The device is maintained or repaired by people not authorized by Eppendorf The user makes unauthorized changes to the device 3 4 Warnings for intended use Read the operating instructions and observe the following general safety information before using the ThermoMixer C DANGER Risk of explosion gt Do not operate the device in areas where work is completed with explosive substances K Do not use this device to process any explosive or highly reactive substances K Do not use this device for processing any substances which could generate an explosive atmosphere DANGER Electric shock as a result of penetration of liquid K Switch off the device and disconnect the power plug before starting cleaning or disinfection work Do not allow any liquids to penetrate the inside of the housing Use closed tubes and closed plates Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside vv v v jenuew Burje1ado Operating manual 10 Eppendorf ThermoMixer C Operating manual WARNING Electric shock due to damage to device or mains cable K Only switch on the device if the device and mains cable are undamaged gt Only use devices
23. e setting Run time 15 s 99 30 h unlimited In the range of 15 s 20 min adjustable in steps of 15 s n the range of 20 min 1 00 h adjustable in steps of 1 min n the range of 1 00 h 10 00 h adjustable in steps of 5 min n the range of 10 00 h 99 30 h adjustable in steps of 30 min 9 5 Interface USB interface For Eppendorf service only Eppendorf ThermoMixer C Operating manual 10 Ordering information CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf 10 1 Device and accessories Order no international Order No North America Description ThermoMixer C basic device without thermoblock 5382 000 015 220 V 240 V u 5382000023 100 V 130 V 5308 000 003 5308000003 ThermoTop with condens protect technology 5363 000 233 5363000233 Lid for ThermoMixer F1 5 ThermoMixer FP for SmartBlocks 0 5 mL 1 5 mL 2 0 mL plates PCR 96 PCR 384 5361 000 031 5361000031 SmartBlock 0 5 mL Thermoblock for 24 t
24. eight dimensions rR e 9 38 9 3 Ambient conditions occ ke RU hh KR RR Hom te 38 9 4 Application parameters es 39 9 4 1 Temperirniga sisse Rm nen a ee RK 39 94 2 MIXING EE 40 9 4 3 CTIME SEHING ai 3 nc tn t 40 Ban Interfaces o is ton etre ret ioi n Ue der Eod dae eco betta oe ds 40 Ordering information 0 0 c eee eect I hh 41 10 1 Device and accessories 0 x e c x e x ccc ee cee ee eee ee hne 41 10 2 Tubes and plates 22s commete nn os 42 10 3 Temperature Sensor cene Ferr RR SEE eA EC ER rir a d x CER EEN 43 Eppendorf ThermoMixer C Operating manual amp User instructions 1 1 this manual gt Read this operating manual completely before using the device for the first time Please also note the operating instructions for the accessories if applicable K This operating manual is part of the product Thus it must always be easily accessible K Enclose this operating manual when transferring the device to third parties K If this manual is lost please request another one You will find the current version on our webpage www eppendorf com worldwide 1 2 Danger symbols and danger levels The safety instructions in this manual appear with the following danger symbols and danger levels
25. ermoblock is not compatible with ThermoTop Use a compatible thermoblock with a condens protect symbol The interface between the device and the ThermoTop is dirty Remove any dirt from the front of the ThermoTop Remove any dirt from the top of the device especially from the viewing window beside the heating cooling plate not mix or control the temperature ThermoTop does The thermoblock is not K compatible thermoblock not fit on the device compatible with ThermoTop with a condens protect The lid is attached to the symbol f thermoblock K Ifusing the ThermoTop do not use the lid The device does Various causes are possible K Contact your local Eppendorf partner 33 jenuew Burje1ado EN Operating manual Eppendorf ThermoMixer C Operating manual 6 2 Error messages recognized with the device The thermoblock is not attached properly Symptom Cause Remedy message Thermoblock not Thermoblock is not compatible Use a compatible thermoblock Remove the thermoblock and then reattach it The interface between the device and the thermoblock is dirty Remove any dirt from the underside of the thermoblock Remove any dirt from the top of the device especially from the viewing window beside the heating cooling plate Error message preceded by a number code Various causes are possible 2 Switch off d
26. erval After 500 operating hours After 1000 operating hours After 2000 operating hours No notification Go to next higher menu level Back Exit menu item without saving Cancel Save set value Save The program keys as well as the Programs and Time Mode menu items are locked while time counting is active 5 9 Programs A program consists of up to four program levels steps The program levels automatically run one after the other You can save separate settings for each program level Mixing time temperature control duration Mixing frequency Temperature Program levels with pause interval Interval Mix Program levels with restricted ramp rates The program ends automatically i The ThermoMixer C has 20 program slots At the end of this operating manual there is a printed form of a program table The program data can be noted there 5 9 1 Creating a program 1 In order to open the menu keep the menu enter key pressed 2 Use the menu arrow keys to select the Programs menu item Confirm with the menu enter key 3 Use the menu arrow keys to select an empty program space Confirm with the menu enter key P OG Cancel Gave lg Options 01 00 or 1000 TC h min rpm 27 jenuew Burje1ado Operating manual 28 Eppendorf ThermoMixer C Operating manual Creating a single level program 1 Set the mixing time temperature and mixing frequency with the time
27. evice and wait 10 seconds Switch on device If the error message appears again contact your local Eppendorf partner E 7 Maintenance 7 1 Setting service intervals The ThermoMixer C offers the option of activating a reminder that the device needs to be Serviced To set a service interval proceed as follows 1 Under Menu Settings select the Service menu item Confirm with the menu enter key 2 Select a service interval with the menu arrow keys after 500 1 000 or 2 000 operating hours To switch off the notification select No notification When the specified operating hours have been reached a message appears Contact your local Eppendorf partner The contact addresses can be found online at www eppendorf com worldwide 34 Eppendorf ThermoMixer C Operating manual 7 2 Cleaning Clean the housing of the ThermoMixer C and the thermoblocks regularly DANGER Electric shock as a result of penetration of liquid K Switch off the device and disconnect the power plug before starting cleaning or disinfection work Do not allow any liquids to penetrate the inside of the housing Use closed tubes and closed plates Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside vv v v NOTICE Damage from the use of aggressive chemicals gt Do not use any aggressive chemicals on the device or its accessor
28. f eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com
29. ies such as Strong and weak bases strong acids acetone formaldehyde halogenated hydrocarbons or phenol K Ifthe device has been contaminated by aggressive chemicals immediately clean it by means of a mild cleaning agent NOTICE Corrosion from aggressive cleaning agents and disinfectants K Do not use corrosive cleaning agents aggressive solvents or abrasive polishes K Do not use lab cleaners with sodium hypochlorite Auxiliary equipment Lint free cloth Mild soap based lab cleaner Dist water Cleaning the ThermoMixer C 1 Switch off the ThermoMixer C and disconnect it from the power supply 2 Allow the device to cool down 3 Clean all of the outer parts of the ThermoMixer C with a mild soap solution and a lint free cloth 4 Wipe off the soap solution with dist water 5 Dry all cleaned parts 7 2 1 Cleaning the thermoblock Auxiliary equipment Lint free cloth Mild soap based lab cleaner Dist water Clean the thermoblock immediately if sample fluid enters the bore holes or comes into contact with the surfaces 1 Clean with a mild soap solution 2 Wipe off the soap solution with dist water 3 Drythe cleaned thermoblock 35 jenuew Burje1ado EN Operating manual 36 Eppendorf ThermoMixer C Operating manual 7 3 Disinfection Decontamination DANGER Electric shock as a result of penetration of liquid K Switch off the device and disconnect the power plug before starting cle
30. ightly before use WARNING Injury from sample material being thrown out Sample material can be thrown out of open improperly sealed or unstable tubes and plates K Only mix in closed tubes and closed plates K Observe the nationally prescribed safety environment when working with hazardous toxic and pathogenic samples Pay particular attention to personal protective equipment gloves clothing goggles etc extraction and the safety class of the lab CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf CAUTION Crush hazard due to moving parts K Do not replace any consumables during the mixing process K Do not remove the thermoblock during the mixing process K Put on the ThermoTop or Lid prior to the mixing process K Do not remove the ThermoTop or Lid during the mixing process 11 jenuew Burje1ado Operating manual 12 Eppendorf ThermoMixer C Operating manual NOTICE Caution Strong vibration When mixing at high speeds items located near the device may be moved by the
31. k i Speaker switched on N Key lock activated parameters cannot be XX Speaker switched off changed y No key lock 9 Time mode 10 Mixing frequency x Time Control Time counting begins immediately Temp Control Time counting begins when the set temperature has been reached 11 Actual temperature 12 Set temperature When the actual temperature flashes on the When the set temperature has been display the device is not in temperature reached only one value is displayed control mode operation 13 Mixing time 15 Eppendorf ThermoMixer C Operating manual EN 5 2 Setting the language The device is delivered with English as the default language To set another language proceed as follows 3 1 Switch on the device with the power switch at the 5 rear of the device E D 2 To open the menu press the menu enter key E 5 3 Select the Settings menu item with the menu 5 arrow keys To confirm your selection press the menu enter key Select the Language menu item with the menu arrow keys Confirm with the menu enter key Select the language with the menu arrow keys and press the menu enter key A tick appears in front of the selected language To exit the menu press the left menu arrow key several times 5 3 Installing the thermoblock WARNING Personal injury or material damage due to chemically or mechanically damaged ther
32. k plates Microplates with various 3 000 rpm ThermoTop or bottom contours Lid Deepwell plates with various 2000 rpm bottom contours up to 80 C Deepwell plates with various 1000 rpm bottom contours from 80 C SmartBlock PCR 96 96 well PCR plates 2 000 rpm ThermoTop or 0 2 mL PCR tubes Lid SmartBlock PCR 384 384 well PCR plates 3 000 rpm ThermoTop or Lid The height sensor of the SmartBlock plates automatically differentiates between deepwell plates and microplates f you are using the Lid select a mixing frequency of max 2 000 rpm 17 jenuew Burje1ado Operating manual Eppendorf ThermoMixer C Operating manual 5 3 1 Attaching the thermoblock 1 First only attach the rear edge of the thermoblock The writing must face to the front 2 Push the front edge of the thermoblock down The thermoblock audibly engages The display shows the name of the thermoblock 5 3 2 Removing the thermoblock WARNING Risk of burns from hot surfaces The thermoblock and the heating cooling plate can be very hot after heating and cause burns K Allow the thermoblock and heating cooling plate to cool down completely before removing the thermoblock 1 To unlock the thermoblock press the lever at the front of the thermoblock down 2 Liftthe front edge so that the thermoblock is tilted backwards 3 Remove the thermoblock upwards 5 4 Inserting tube
33. mL Conical tubes with volumes of 15 mL and 50 mL Microplates and deepwell plates with any kind of bottom shape PCR plates e g Eppendorf twin tec PCR Plate 96 Eppendorf twin tec PCR Plate 384 Tubes with a diameter of 11 mm to 11 9 mm Cryo tubes Temperature control Peltier cooling allows samples to be cooled to 15 C below the ambient temperature The temperature range can be set from 1 C to 100 C Mixing Depending on the thermoblock being used you can select mixing frequencies between 300 rpm and 3 000 rpm Anti spill technology prevents lid wetting and cross contamination e 2DMix Control technology provides for controlled and efficient mixing to ensure rapid and complete mixing of even the smallest volumes Short Mix Short uncomplicated mixing of sample material The mixing process runs at the selected speed as long as the short key is pressed Interval Mix Continuous switching between mixing phases and pauses The mixing frequency and the duration are freely selectable Interrupt time counting If you want to add reagents or exchange tubes while mixing you can interrupt time counting and the mixing process Multi level mixing temperature control n addition to a normal mixing temperature control run you can freely define programs with up to four successive levels steps The program levels automatically run one after the other Atotal of 20 program slots are available Program
34. mmediately starts to perform the temperature control 3 Setthe mixing frequency with the speed arrow keys quy 01 00 1000 TC h min rpm 4 In order to start the mixing process press the start stop key The symbol flashes on the display The mixing time is counted down The display shows the remaining mixing time the actual temperature set temperature and the mixing frequency Eppendorf ThermoMixer C Operating manual EN 5 After the set mixing time has elapsed the device stops automatically A signal tone sounds The display shows the last used parameters Temperature control is continued o a 5 7 3 Mixing temperature control with continuous run a 3 0 Icing of the thermoblock EZ Ice may form on the thermoblock if samples are tempered at low temperatures for a 3 long time 3 c E 1 In order to mix without any time limits use the time arrow keys to select the setting v below 15 s or A above 99 30 h 2 Setthe temperature with the temp arrow keys The device immediately starts to perform the temperature control 3 Setthe mixing frequency with the speed arrow keys 1000 C rpm 4 In order to start the mixing process press the start stop key The symbol flashes on the display The display alternately shows the mixing time and the o symbol the act
35. moblocks K Do not use thermoblocks that show signs of corrosion or mechanical damage K Regularly check the condition of the thermoblocks WARNING Contamination due to opening seals of consumables In the following cases the seals of tubes can spring open Sample material can escape high vapor pressure of the content improperly sealed cover damaged sealing lip improperly fastened foil K Always check that consumables have been sealed tightly before use When you attach the thermoblock the device automatically recognizes the mounted thermoblock The mixing frequency is automatically limited to the maximum value for the thermoblock being used Only the following thermoblocks can be used with the ThermoMixer C Exchangeable thermoblocks for the Thermomixer comfort Thermomixer R are not compatible 16 Eppendorf ThermoMixer C Operating manual Thermoblock Tubes Plates Maximum mixing Accessories frequency SmartBlock 0 5 mL Tube volume 0 5 mL 2 000 rpm ThermoTop or Lid SmartBlock 1 5 mL Tube volume 1 5 mL 2 000 rpm ThermoTop or Lid SmartBlock 2 0 mL Tube volume 2 0 mL 2 000 rpm ThermoTop or Lid SmartBlock 5 0 mL Tube volume 5 0 mL 1 000 rpm SmartBlock 12 mm Tubes with a diameter 2 000 rpm of 11 mm to 11 9 mm SmartBlock cryo Cryotubes 2 000 rpm SmartBlock 15 mL Conical tubes volume 15 mL 1 000 rpm SmartBlock 50 mL Conical tubes volume 50 mL 1 000 rpm SmartBloc
36. p key Start or stop Setting the mixing frequency mixing temperature control Keep the arrow key pressed Quick setting Press and hold the start stop key for 2 s Pause interrupt mixing process and time counting 5 Arrow keys temp Arrow keys time Set temperature Setting the mixing time Keep the arrow key pressed Quick setting Keep the arrow key pressed Quick setting As soon as the target temperature is modified the device begins to perform temperature control 7 Display Menu arrow keys Navigating the menu Load or edit programs set key lock set the time mode edit the settings 9 menu enter key Open the menu Confirm your selection Eppendorf ThermoMixer C Operating manual EN 3 6 7 8 9 P 06 ELISA Oe wir lt ET Step Step Step E 3 1 00 EIER EUU h min T rpm 7 13 12 11 10 Fig 3 Display ThermoMixer C 1 Program number 2 Program name 3 Program levels step 1 to step 4 4 Device status current step lt x Device is performing mixing temperature control lI Mixing process interrupted temperature control to be continued 5 ThermoTop 6 Interval Mix Pi ThermoTop has been attached Interval Mix has been activated for the To prevent the formation of condensate the current step device heats up the ThermoTop before the temperature of the thermoblock is controlled 7 Speaker 8 Key loc
37. s and plates WARNING Risk of injury due to incorrect consumables Poorly fitting tubes or plates can become detached from the thermoblock Glass tubes can smash K Only use the thermoblocks with the consumables designed for them K Never use tubes made of glass or other fragile material 18 Eppendorf ThermoMixer C Operating manual NOTICE Damage to plates due to too high temperatures Polystyrene microplates melt at temperatures above 70 C Polypropylene deepwell plates deform at temperatures above 80 C Deformed plates can become detached from the thermoblock K Only heat microplates up to 70 C K If you are heating deepwell plates above 80 C do not exceed the mixing frequency of 1 000 rpm NOTICE Material change of consumables due to extreme temperatures Extreme temperatures e g during refrigeration or autoclaving affect consumables material The mechanical strength dimensions and shape of the consumable will change Use consumables that are suitable for the selected temperature range or selected procedure jenuew Burje1ado i The height sensor of the SmartBlock plates automatically differentiates between deepwell plates and microplates K When inserting microplates make sure that the height sensor is not covered K Take care that the height sensor does not get contaminated K Place the suitable thermoblock on the device see Attaching the thermoblock on p 18 Inserting the pla
38. te K Insert the plate with the back edge first Then press it down at the front Inserting tubes K Insert the tubes completely into the bores of the thermoblock SmartBlock plates SmartBlock PCR 96 or SmartBlock PCR 384 To ensure uniform temperature control place the lid on the thermoblock 1 Height sensor 19 Operating manual 20 Eppendorf ThermoMixer C Operating manual 5 5 Installing the ThermoTop The ThermoTop is compatible with thermoblocks which feature the condens protect symbol The condens protect technology available with ThermoTop prevents the formation of condensation on the inner wall or the lid of the tube Prerequisites A compatible thermoblock has been attached Tubes or plates have been inserted Place the ThermoTop on the device vertically from above The centering pins behind the heating cooling plate fit into the recesses of the ThermoTop The ThermoTop is correctly positioned if the seal is fully flush with the upper part of the device The blue LED of the ThermoTop lights The f symbol appears in the display 0 Functioning principle of the ThermoTop In order to prevent the formation of any condensate in a reliable manner the device first heats the ThermoTop until it reaches the set temperature The tempering of the thermoblock occurs with a delay The temperature sensor of the thermoblock reacts to the temperature
39. that have been properly installed or repaired K Incase of danger disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or by using the isolating device intended for this purpose e g emergency stop switch in the laboratory WARNING Lethal voltages inside the device K Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident K Do not remove the housing of the device K Do not allow any liquids to penetrate the inside of the housing K Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf WARNING Risk from incorrect supply voltage K Only connect the device to voltage sources which correspond to the electrical requirements on the name plate K Only use sockets with a protective earth PE conductor and suitable power cable WARNING Risk of burns from hot surfaces The thermoblock and the heating cooling plate can be very hot after heating and cause burns K Allow the thermoblock and heating cooling plate to cool down completely before removing the thermoblock WARNING Personal injury or material damage due to chemically or mechanically damaged thermoblocks K Do not use thermoblocks that show signs of corrosion or mechanical damage K Regularly check the condition of the thermoblocks WARNING Damages to health due
40. the mixing time temperature and mixing frequency with the time temp and speed arrow keys 2 Keep one of the program keys prog 1 to prog 5 pressed for 2 seconds Asignal tone sounds The LED above the program key lights blue The parameters of the program are saved 0 f you want to assign a program name save the program in the menu Menu Programs f you want to save a program with multiple levels under the program numbers 1 to 5 select the program space under Menu Programs Eppendorf ThermoMixer C Operating manual 5 9 5 Loading a saved program Loading program prog 1 to prog 5 On supply the prog 1 to prog 5 program keys are assigned as follows Program name Temperature Mixing time Mixing temperature frequency control duration prog 1 key Cooling 8 C oo D rpm prog 2 key Ligation 16 C 16 00 h D rpm prog 3 key Restr Digest 37 C 1 00 h 1 000 rpm prog 4 key Prot K Digest 56 C 10 00 min 1 000 rpm prog 5 key Denaturation 95 C 30 00 min D rpm 1 In order to call up a program on the program spaces 1 to 5 press one of the program keys prog 1 to prog 5 The LED above the program key lights blue The display shows the parameters of the program 2 In order to start the program press the start stop key Loading a program from the program list 1 Inorder to load a program from the program list select the program under Menu Programs Confirm with the menu enter key
41. thermoblock can be measured Details on the verification process with the Eppendorf Temperature Verification System Single Channel can be found in the corresponding operating manual Eppendorf ThermoMixer C Operating manual EN 8 Transport storage and disposal E 8 1 Transport Ei CAUTION Bodily injury due to lifting and carrying heavy loads a The device is heavy Lifting and carrying the device can lead to back injuries Si K The device must be transported by least two people K Use a transport aid e g dolly to transport the device longer distances 3 to K Use the original packaging for transport E to Air temperature Relative humidity Atmospheric pressure General transport 25 C 60 C 10 75 30 kPa 106 kPa Air freight 40 C 55 C 10 75 30 kPa 106 kPa 8 2 Storage Air temperature Relative humidity Atmospheric pressure In transport packaging 25 C 55 C 10 96 95 70 kPa 106 kPa Without transport 5 0 45 C 10 96 95 70 kPa 106 kPa packaging 8 3 Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According
42. time counting press the start stop key The symbol flashes on the display The temperature control duration is counted down The display shows the remaining temperature control duration and the actual temperature set temperature A signal sounds after the temperature control duration has elapsed e 21 Operating manual 22 Eppendorf ThermoMixer C Operating manual 5 7 Mixing You can select the mixing frequency between 300 rpm and 3 000 rpm The mixing frequency can be adjusted in steps of 50 rpm 5 7 1 Mixing without temperature control 1 In order to switch off the temperature control use the temp arrow keys for selecting the off setting v below 1 C or A above 100 C EIL MES 01 00 ott 1000 h min TC rpm 2 Set the mixing time by means of the time arrow keys Set the mixing frequency by means of the speed arrow keys 4 In order to start the mixing process press the start stop key The symbol flashes on the display The mixing time is counted down The display shows the remaining mixing time the actual temperature set temperature and the mixing frequency e 5 After the set mixing time has elapsed the device stops automatically A signal tone sounds The display shows the last used parameters 5 7 2 Mixing and tempering 1 Setthe mixing time with the time arrow keys 2 Setthe temperature with the temp arrow keys The device i
43. tion in our catalog or on our website www eppendorf com 10 3 Temperature sensor Order no international Order No North America Description 0055 000 298 950008059 Temperature Verification System Single channel for Mastercycler Mastercycler ep Mastercycler pro and Mastercycler nexus Thermomixer compact Thermomixer comfort Thermostat plus 43 jenuew Burje1ado Operating manual 44 Eppendorf ThermoMixer C Operating manual Index b A Actual temperature see ee ee eee ee ee eee 15 Application parameters 39 C ET UE 35 Condens Drotert 20 Continuous mun 23 D Decopntaminaton 36 Deepwell plate 6 16 18 DIS Play ic ci cn c tree e xe 7 15 Ree ET 3 3 3 3 131 0 37 G GOSS m as 6 H Hazard Sossar birns sidns eet 9 Heating cooling plate 7 Installation H T 13 Selecting the location 13 Intended use sas icr err a ee hat iie 9 Interval Mix Set Symbol Key lock Symbol Language Set Navigation ca aire decretis Overview nn Setting the language Microplate eene MIXING 33706070 0 0 70 Application parameters Mixing frequency Mixing lan ciere tice Setting the mixing
44. to these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste To document this they have been marked with the following identification Because disposal regulations may differ from one country to another within the EU please contact your supplier if necessary 37 Operating manual Eppendorf ThermoMixer C Operating manual 9 Technical data 9 1 Power supply Power connection 100 V 130 V 10 50 Hz 60 Hz 220 V 240 V 10 50 Hz 60 Hz Power consumption Maximum 200 W Overvoltage category Degree of pollution 2 Protection class 9 2 Weight dimensions Dimensions Width 20 6 cm 8 1 in Depth 30 4 cm 12 0 in Height 13 6 cm 5 4 in Weight 6 3 kg 13 9 Ib 9 3 Ambient conditions Ambience Only for use indoors Ambient temperature 5 C 40 C Relative humidity 10 90 non condensing Atmospheric pressure 79 5 kPa 106 kPa Eppendorf ThermoMixer C Operating manual 9 4 Application parameters 9 4 1 Tempering Temperature control range Minimum 15 C 2 C below ambient temperature Maximum 100 C Temperature setting 1 C 100 C adjustable in steps of 1 C Temperature accuracy Set temperature Set temperature 20 C 45 C lt 20 Cor gt 45 C Smart
45. ture and mixing frequency on a free program space You can save up to 4 sets of parameters as automatically consecutive program levels steps 25 jenuew Burje1ado Operating manual Eppendorf ThermoMixer C Operating manual Repetitions Language Contrast 0 Set repetitions of the signal tone 1 x 5 x 10 x 30 x Unlimited Set the language English German French Italian Spanish Set the contrast 0 25 96 50 75 100 Menu items and options Description Symbol on the display Edit program Overwrite saved program or save it to a new program space Delete program Delete the saved program Programs 1 to 5 cannot be deleted You can edit and overwrite the programs Key lock Key lock on Parameters cannot be changed H Key lock off Parameters can be changed d Time mode Time Control Time counting and mixing process begin a immediately Temp Control Time counting and mixing process only begin 2 when the set temperature has been reached Settings Signal tones The signal tone for error messages is always output at medium volume level regardless of the speaker settings Volume Setthe volume of the speaker il 20 40 60 80 100 Switching the speaker off Xx 26 Eppendorf ThermoMixer C Operating manual Menu items and options Description Symbol on the display Service Set the service int
46. ual temperature set temperature and the mixing frequency The mixing time is counted up 5 n order to end the mixing process press the start stop key Asignal sounds The display shows the last used parameters Temperature control is continued A mixing time of more than 99 30 h is possible After 99 30 h has passed the display only will show the o symbol 23 Operating manual 24 Eppendorf ThermoMixer C Operating manual 5 7 4 Interrupting the mixing process If you want to add reagents or exchange tubes while mixing you can interrupt the mixing process The temperature control continues to run during the pause 1 In order to interrupt the mixing process keep the start stop key pressed for 2 s T EHP Pause 3r 1000 h min T rpm The display shows Pause The mixing process is interrupted Time counting is stopped Temperature control is continued 2 In order to continue the mixing process press the start stop key 5 7 5 Short Mix Use the Short Mix function for mixing for a short while without temperature control 1 Setthe mixing frequency by means of the speed arrow keys 2 Keep the short key pressed The mixing process continues as long as the short key will be pressed 3 In order to end Short Mix release the short key 5 7 6 Mixing tempering with Time Control or Temp Control You can specify when time counting should begin Time counting
47. ubes 0 5 mL 5360 000 038 5360000038 SmartBlock 1 5 mL Thermoblock for 24 tubes 1 5 mL 5362 000 035 5362000035 SmartBlock 2 0 mL Thermoblock for 24 tubes 2 0 mL 5309 000 007 5309000007 SmartBlock 5 0 mL Thermoblock for 8 tubes 5 0 mL 5366 000 021 5366000021 SmartBlock 15 mL Thermoblock for 8 conical tubes 15 mL 5365 000 028 5365000028 SmartBlock 50 mL Thermoblock for 4 conical tubes 50 mL 5364 000 024 5364000024 SmartBlock 12 mm Thermoblock for 24 tubes diameter 11 mm 11 9 mm height 34 mm 76 mm 5367 000 025 5367000025 SmartBlock cryo Thermoblock for 24 Cryo tubes 1 5 2 mL diameter max 12 5 mm all base shapes 41 jenuew Burje1ado Operating manual 42 Eppendorf ThermoMixer C Operating manual Order no Order No Description international North America 5363 000 039 5363000039 SmartBlock plates Thermoblock for microplates and deepwell plates incl Lid 5306 000 006 5306000006 SmartBlock PCR 96 Thermoblock for PCR plates 96 incl Lid 5307 000 000 5307000000 SmartBlock PCR 384 Thermoblock for PCR plates 384 incl Lid 10 2 Tubes and plates Order no Order No Description international North America 0030 121 023 02236361 1 Eppendorf Safe Lock Tube 0 5 mL 500 pieces clear 0030 120 086 Eppendorf Safe Lock Tube 1 5 mL 1 000 pieces clear 0030 120 094 Eppendorf Safe Lock Tube 2 0 mL 1 000 pieces clear 0030 124 502
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