H301-EC-LG-UP-BL User Manual
Contents
1. UP BL User Manual Lock Spring Closed Figure 5 Replace of the Specimen Holder inside the Chamber Base www oko lab com November 2014 ast H301 EC LG UP BL User Manual November 2014 6 Working with 35 or 60 mm Petri Dishes Flat springs prevent movement of 35 and 60 mm dishes inside of the sample holder See Figure 6 and Figure 7 Water Reservoir Figure 7 Plate adapter for 60 mm Petri dish holder Note On the sample holders for petri dishes there are two water reservoirs see Image 2 of Figure 6 and Figure 7 Fill them with water to ensure a high humidity percentage inside the chamber www oko lab com ew H301 EC LG UP BL User Manual November 2014 7 Working with 1x3 chamber slides Flat springs prevent movement of 1 x 3 chamber slides inside of the sample holder A and B in the Figure 8 below Temperature reference reservoir Figure 8 Plate adapter for 1 x3 chambered cover glass holder Note On the sample holders for chamber slide there is a Temperature reference reservoir When operating in Sample Feedback Mode fill it with water and immerse the temperature sensor This acts as a reference well allowing sample temperature feedback without contaminating the sample 8 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 EC LG UP BL Silicon tubing con nects to a gas input brass opening loc2. H301 EC LG UP BL User Manual November 2014 H301 EC LG UP BL Compatible with the following Z Piezo stages Compatible with the following Okolab Controllers LEICA Super Z Galvo stage SP5 SP8 H301 T BL PLUS UNO COMBINED CONTROLLER H401 T DUAL www oko lab com Page 1 H301 EC LG UP BL User Manual November 2014 Index 1 Components and GIMENSIONS necictsciesteias rinses escent est a eee De Sampe Rold Ss x tect ete cece E EET E eet tec eat ee eet 2 1 Available Sample HoldersS essessessossossoesosossosesoeosessesoesoesoesoesossoesoesoesossoesoesoosossoosossossossoosoo 3 Insertion of the Sample Feedback Temperature SenSor sessesescecescecesoecesoecesoecesoecesoecesoeceseececeeeo 4 Insertion of Sample Holder into Chamber esesesesescesecesesesesesoscecesececesesescecesecesesesesessecececeseseseoo Ds Same TOIGGr FOO ACCUM ai cos a creer ncaa a a S E ciaenencueiaeuseeereeaceee 6 Working with 35 or 60 mm Petri DNS NC Saas ciate sas ecertesee crs scicae cise cree care care sarees cae sie eare ls Wo rtkinge with 1x3 chamber SUNG S crear sco crac carne cars Gserticscae creas creas Grsas ar a ce ae eeureeaam seen ceseees Be CONMIMGCELO On Cine GaS SUDD ssa rs oars Soa osc chess gcc cg sas es rs tre ws re cs ee Ghee we es teen cen ceases 2 WONG IIS VEROS Macaca ny sco caro ae creva areas 10 59 creas Grose a arse ee ae aera a a ee aniees 10 Connection of the Chamber with Super Z Galvo stage
3. ated on the side of the H301 EC LG UP BL See Figure 9 Connect by gently pushing silicon tubing onto brass opening Gas Input Figure 9 Connection with gas supply 9 Working with Perfusion The H301 EC LG UP BL features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2 0 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as needed before introducing perfusion tubing Figure 10 shows the location of perfusion holes Front and 3D views www oko lab com page H301 EC LG UP BL User Manual November 2014 1 Frontal view 2 3D view Figure 10 Perfusion 10 Connection of the Chamber with Super Z Galvo stage H301 EC LG UP BL fits on the Super Z Galvo stage It can be screwed onto the Galvo Stage as shown in Figure 11 place the chamber on the stage and screw the four slotted head pan screws Screws housings are indicated with letter A in Figure 11 Screw M2x6 Figure 11 How to fix the H301 EC LG UP BL on the Super Z Galvo stage www oko lab com Page9
4. back Temperature Sensor through one of 12 perfusion holes see par 9 Figure 10 located in the H301 EC LG UP BL chamber body see Figure 3 Front and 3D views Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screw as needed before introducing the sample Feedback Temperature Sen Sor Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 3 Insertion of the temperature sensor inside the chamber www oko lab com Paget H301 EC LG UP BL User Manual November 2014 4 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by 2 Captive screws M2x6 as shown in Figure 4 Images 1 and 2 Figure 4 Introduction of the specimen Holder in the Chamber Base 5 Sample Holder replacement If you want to change the sample holder follow the steps shown in the images of Figure 5 and listed below 1 2 3 4 5 6 Open the lock spring as shown in Figure 5 Image 2 Open the chamber lid as shown in Figure 5 Image 3 Remove the chamber by lifting it from the workspace as shown in Figure 5 Image 4 Remove the chamber by lifting it from workspace as shown in Figure 5 Images 5 and 6 Unscrew captive screws The Captive screw location is indicated by letter A in Figure 5 Image 7 Lift and replace the sample holder as shown in Figure 5 Image 8 ie www oko lab com H301 EC LG
5. esesesescececececesesescecesecececesesescececececeseseese www oko lab com es H301 EC LG UP BL User Manual November 2014 1 Components and dimensions H301 EC LG UP BL includes the following components Chamber base with embedded temperature sensor Chamber body with holes x12 for the insertion of perfusion tubing up to 2 0 mm outer diameter Objective adapter Please specify objective in use and adapter dimensions at the time of purchase Heated Lid It features a hinge system allowing opening and closing the lid around the objective Total chamber weight 170 g Objective adapter 28 30 O Chamber Body Sample Holder Order Separately Chamber top view Chamber Base Figure 1 H301 EC LG UP BL Components and Dimensions www oko lab com pape H301 EC LG UP BL User Manual November 2014 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish holder 1 standard chamber slide holder 1 60mm Petri dish holder H301 EC LG UP 1x35 H301 EC LG UP 1xGS H301 EC LG UP 1x60 For 1 35mm Petri dish holder For 1 60mm Petri dish holder For 1 1 x3 chamber slide holder Figure 2 Available sample holders 3 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feed
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