Biosum User Manual
Contents
1. If Biosum is left unused during a longer period it can be necessary to reset date and time Date and time can also be set very easily by the PC software SIDENA 502 avenue de Neuville 78950 Gambais JPD 17 07 07 7 Il Jen 8 01 34 87 19 05 201 34 87 19 06 Sidena 24 06 2007 User Manual Ver 1 2 Connecting to a PC Copy the file Biosum_XFER EXE to a directory in a Windows PC Switch the Biosum on and strike function XFER in the main menu Launch the program Biosum_XFER on the PC The PC tries to connect to Biosum If it succeeds one sees connected displayed at the top of the PC screen and on the Biosum screen If not there is a warning message In this case check that the right serial port is selected if necessary change it and click again the button Connect To finish the transfer and to send Biosum back to its main menu click the button Disconnect or exit the PC program The PC Program shows the operating mode of the Biosum and the last counted key On the PC screen the TRANSFERS group gives the possibility to receive data from Biosum transfer these data to an Excel spreadsheet erase the data in Biosum memory export the data as a text file The TOOLS group allows to set Biosum date and time the PC date and time are then automatically transferred modify the names and mnemonics of the Biosum grid cells Changing names and mnemonics The windo2. Sidena 24 06 2007 User Manual Ver 1 2 BIOSUM DIFFERENTIAL CELL COUNTER USER MANUAL SIDENA 302 avenue de Neuville 78950 Gambais JPD 17 07 07 7 info sidena com 01 34 87 19 05 01 34 87 19 06 Sidena 24 06 2007 User Manual Ver 1 2 Overview Biosum is a stand alone differential cell counter which facilitates observation counting and classification of cells into 24 different categories It can store results in its own memory transfer them into a PC and or print directly to a serial printer The PC software provided with Biosum can transfer the results to an Excel sheet and it gives the possibility to rename the names and mnemonics of all the keys for specific uses Operating modes Biosum is particularly designed for the characterization of spermatozoa and it shows the results according to the David method or according to the Kruger method It can also be useful for hematology or generally for any application requiring the classification of cells or tissues Kruger Method Professor Kruger s method takes into account only the main anomaly of a spermatozoon At the end of the counting Biosum displays the percentage of typical forms David Method The method described by Professor David counts the spermatozoa by noting up to six abnormalities per specimen and calculates the IAM multiple anomalies index The screen cells display the number of times when the abnormality has been noted and the number of times it was a
3. al or typical cells must be counted by striking key 18 TYPICAL Key 6 has no effect Function key ABSO requires to enter the number of white blood cells and then displays the absolute values Keys 23 and 24 do not increase the total counting These keys are just used to record characteristics elements which must not be taken inside the cumulated number of cells The last key inside then main count is thus key 22 but it can be changed by the PC software Its value must be between 19 and 24 in the upper key line SIDENA 302 avenue de Neuville 78950 Gambais JPD 17 07 07 7 info sidena com 01 34 87 19 05 01 34 87 19 06 Sidena 24 06 2007 User Manual Ver 1 2 The other menus SETUP menu ESC sends back to the main menu MODE selects the operating mode o Hematology counting o Spermatozoa counting following DAVID method o Spermatozoa counting following KRUGER method BEEP sounds when striking a key CSTD resets the standard key names one can change cells names and mnemonics with the PC software DATE sets date and time it can also be done by the PC software IMPR selects the type of serial printer connected to Biosum if any Setting date and time Enter the date following the format indicated obave including character For entering time the separator character is In case of bad keying the clock will not start Date and time are hold by a small internal battery during about fifteen days
4. ermatozoa must be counted with key 18 TYPICAL key 6 as no effect striking keys 19 to 24 upper line do not increase the cumulated total counting These keys are used to record elements outside the main counting isolated flagella for example the line at the bottom of the screen shows the number of typical spermatozoa the number of atypical spermatozoa and the percentage of typical The DAVID method the normal elements must be counted directly by sriking on key 18 TYPICAL This increases the content of the corresponding grid cell the abnormalities are noted by striking one or more abnormality keys an asterisk character appears in the corresponding screen cell One can note up to 6 abnomalities on one spermatozoon the abnormalities are then validated by the key 6 ENTER This increments the first figure in the abnormalities cells and erases the asterisks The second figure of each cell indicates the number of times where this abnormality was associated with one or more other abnormalities keys 19 to 24 top line do not increase the total counting The corresponding cells are immediately incremented by each striking these keys are used to record elements outside main counting isolated flagella for example The bottom of the screen displays the index of multiple anomalies IAM Hematology counting Striking any key increments the contents of the corresponding screen cell The norm
5. n or completely by the ERASE function Counting One must initially enter the identification of the sample It can have up to 8 characters including figures 0 to 9 and slash The function key DEL erases the last character function key ESC goes back to the main menu The OK function key validate the identification and starts counting The total number of cells typical and atypical is displayed at bottom right of the screen followed by the character and by the target number to be reached When starting the counting the target number is equal to 100 When one reaches with this number Biosum sounds and gives a warning It is possible then to confirm the end of the counting or to add one or more times 50 to the target number by striking the key 50 Counting can then be continued Thus if one decides to count until 200 cells one can press 2 times on the key 50 before starting counting It is possible to stop counting at any time by striking then END function key Then the counting can be validated continued or aborted The DEL key cancels the last pressed key key displays the results as percentages SIDENA 302 avenue de Neuville 78950 Gambais JPD 17 07 07 7 info sidena com 01 34 87 19 05 01 34 87 19 06 Sidena 24 06 2007 User Manual Ver 1 2 The KRUGER method each key increments the content of the corresponding grid cell on the screen the normal sp
6. ssociated with one or more other abnormalities Hematology mode The absolute values can be displayed after entering the number of white cells The keyboard The keyboard comprises 30 keys divided into 6 function keys the 6 keys closest to the screen and 24 counting keys divided into 4 lines of 6 keys The key 1 is placed bottom left side key 6 is bottom right and so on until the 24th key which is placed top right side Generally key 25 is the ESCAPE key and key 30 is the validation key ENTER 25 26 27 28 29 30 19 20 21 22 23 24 13 14 15 16 17 18 7 8 9 10 11 12 1 2 3 4 5 6 SIDENA 302 avenue de Neuville 78950 Gambais JPD 17 07 07 7 info sidena com 01 34 87 19 05 01 34 87 19 06 Sidena 24 06 2007 User Manual Ver 1 2 Getting started The AC adapter is suitable for supplies from 100 to 260 VAC Plug in the adapter and switch the Biosum on with the switch placed at the rear of the case It displays the following screen which is the Main Menu The upper line shows the functions available with the 6 keys functions The actions will change according to the situation Here we have the following set of choices QUIT to leave READ to read the formulas recorded in memory SETUP to configure Biosum XFER to connect to a PC ERASE to ERASE all the formulas recorded in memory COUNT to start a new counting Formulas in memory can be erased one by one inside the READ functio
7. w which appears when striking button Edit Grid shows the mnemonics and the full names of the cells It also displays the last counted key that is to say the last key that increases the total number the following keys will not see above the 3 operating modes This key can be changed only when in the hematology mode and it must remain beetweem 19 and 24 While clicking modify one can change all the elements of the grid One must then transfer the new values to the Biosum by clicking button Send to Biosum These sets of names can also be saved onto the disc and be reloaded Each of the three operating modes can have its owm set of names saved on disk SIDENA 302 avenue de Neuville 78950 Gambais JPD 17 07 07 7 info sidena com 01 34 87 19 05 01 34 87 19 06
Download Pdf Manuals
Related Search