Hybridization Ovens User Manual
Contents
1. 32 kW OV3 0 31 kW OV1 2 Fuses 2 x 2 0 AT OV1 2 230 Volt 2 x 3 15 AT OV3 230 Volts 2 x 4 AT OV5 230 Volts 2 x 3 15 AT OV1 2 115 Volts 2 x 4 0 AT OV3 115 Volts 2 x 8 AT OV5 115 Volts class IP20 ambient conditions 15 C to 30 C lt 85 relative humidity 1 3 5 Dimensions B x T x H Outer dimensions 433 x 400 x 432 mm OV1 OV2 433 x 400 x 605 mm OV3 Chamber dimensions 355 x 240 x 245 mm OV1 OV2 355 x 240 x 400 mm OV3 355 x 240 x 245 mm OV5 Weight 20 05 kg OV1 21 25 kg OV2 excluding bottle and mains cable 26 05 kg OV3 36 25 kg OV 5 433 x 400 x 726 mm OV5 1 4 Legal Notes 1 4 4 Copyright All rights reserved It is not allowed to copy and publish the manual or parts of it in any form as copies micro film or other methods without a written authorisation from Biometra Biometra is pointing out that applied company and brand names are usually protected trade marks 6 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company 1 4 2 Liability Biometra is not liable for damages and injuries caused by use not considering these operating instructions in parts or completely 1 4 8 Meaning of the Instructions Biometra recommends that you first read these instructions carefully This operation instruction is part of the product and should be kept over the full life time of the instrument It shoul2. from the rotor clamps Instruction Manual Hybridisation Ovens 10 2009 13 Biometra An Analytik Jena Company 4 7 Auto Restart The hybridization ovens OV1 OV2 OV3 and OV5 have an auto restart function In case the mains power supply is interrupted for a few seconds up to several hours the oven will restart automatically as soon as the power is present again The last temperature set will be regulated to 4 8 Working at ambient temperature For working at ambient temperature the value should be set to about five degrees below the ambient temperature in the laboratory The controller this way will never switch on the heater 14 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company 5 Accessories Hybridisation bottle 300 x 35 mm 052 001 Hybridisation bottle 150 x 35 mm 052 002 Hybridisation bottle 100 x 35 mm 052 003 Spare lid incl O ring 052 011 O ring for lid 052 012 Meshes 5 pieces 230 x 230 mm 052 005 Meshes 5 pieces 150 x 100 mm 052 105 Instruction Manual Hybridisation Ovens 10 2009 15 Biometra An Analytik Jena Company 6 Service Should you have any problems with this unit please contact our service department or your local Biometra dealer Biometra GmbH Service Department Rudolf Wissell StraBe 14 16 D 37079 G ttingen Phone 49 0 5 51 50 686 10 or 12 Fax 49 0 551 50686 11 e mail Service biometra com If you would l
3. housing of the oven disconnect it from the mains power Danger by electric shock Open vessels with aqueous solutions must not be used in the oven Vapor may condense on inner surfaces and penetrate into drive and electronics Biometra hybridisation ovens contain no user serviceable parts Do not open the instrument housing Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company Do not use the instrument when damages of the housing block cable or other parts are visible This instrument is designed and certified to meet EN 61010 1 safety standards It should not be modified or altered in any way Alteration of this instrument will void the warranty void the EN61010 1 certification and create a potential safety hazard The instrument must not be used to dry temperate or shake substances that may lead to concentrating an explosive gas inside the oven When using Biometra hybridisation bottles a temperature of 70 C Celsius must not be exceeded Closed vessels must be suited for the vapor pressure resulting from the temperature chosen Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains Connect the hybridisation oven to a grounded socket Appropriate safety regulations must be observed w
4. hybridisation oven on a stable surface in a dry safe environment e Let equilibrate the hybridisation oven to room temperature before starting operation 1 to 6h e Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains e Connect the hybridisation oven to a grounded socket e The maximum temperature that can be used in the Biometra hybridization ovens OV 1 2 3 or OV 5 is 85 C Higher values should not be set as the built in temperature fuse will switch off the heater at about 90 degrees The fuse will start the heater again after cooling down Thereby of course an undefined temperature regulation with temperature fluctuating a lot will result Note When opening the doors rotor shaking platform fan and heater will be switched off from safety reasons The temperature setting will remain in store and the oven will heat to the set temperature again as soon as the door is closed e The rotors in the incubation chambers can hold up to 6 large bottles 300 x 35 mm or six small bottles 150 x 35 mm or 100 x 35 mm Any combination of large and small bottles can be used The rotor speed can be set from about 1 to 9 5 revolutions per minute For longest lifetime of the drive system the rotor should be loaded as balanced as possible e g when 10 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company three bottles are inc
5. 16 6 1 Instructions Tor return shipment 2 ee 16 Instruction Manual Hybridisation Ovens 10 2009 3 Biometra An Analytik Jena Company 62 Equipment Decontamination Certificate 17 7 Note for the disposal of electric electronic waste e 19 8 EU Konformitatserklarung EU Declaration of Conformity 20 9 TTT 21 10 GET M AR 22 4 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company 1 Introduction 1 1 Field of Applications The hybridization ovens described herein have been designed for incubation of materials in closed and heat resistant vessels bottles basins within the conditions described in chapter 1 3 4 technical data The ovens are not suited for incubation in open vessels or for drying of glasses or other things 1 2 Special features 1 2 1 Vacuum double glass door The ovens OV2 3 und 5 have a metal framed double glass vacuum door The door protects the user as far as possible from low energy radiation like B emission and provides for optimal thermal insulation 1 2 2 Rotor and oven interior All ovens have at least one rotor that can be equipped with different size spring clamps and therefore offers maximal flexibility Underneath the rotor is drip tray made of stainless steel that safely collects spills that may occur Moreover the complete oven interior is line
6. Biometra An Analytik Jena Company Hybridisation Ovens Instruction Manual Ver 09 10 Modell Best Nr Mini Hybridisation Oven OV1 230V 052 000 Mini Hybridisation Oven OV1 115V 052 090 Mini Hybridisation Oven OV2 230V 052 100 Mini Hybridisation Oven OV2 115V 052 190 Mini Shaking Hybridisation Oven OV3 230V 052 200 Mini Shaking Hybridisation Oven OV3 115V 052 290 Duo Therm Hybridisation Oven OV5 230V 052 300 Duo Therm Hybridisation Oven OV5 230V 052 390 Please read these instructions carefully before using this apparatus Biometra GmbH Rudolf Wissell Str 30 Service Department D 37079 G ttingen Rudolf Wissell Strasse 14 16 Tel 4449 0 551 50 68 6 0 D 37079 G ttingen Fax 4449 0 551 50 68 6 66 Tel 49 0 551 50 68 6 10 or 12 email Info biometra com Fax 4449 0 551 50 68 6 11 Internet http www biometra com email Service biometra com Biometra An Analytik Jena Company This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change 2 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company Contents 1 PEDRO Ib 1 D 5 1 1 Field of Applications E 5 l 2 Speal feature HT TR 5 1 2 1 Vacuum double glass door ana Edad iR i PERI Ras RUE 5 1 2 2 Rotor and oven EE 5 1 2 3 Shaking PA OPI ade Soxralud ded eds dsc mtus orsi Siret i cep lagi adsis ua Rc mmed vasa eai ER Mde 5 UR WR e
7. d also be forwarded to subsequent owners and users Make sure that additions and updates are inserted into the operation instructions Instruction Manual Hybridisation Ovens 10 2009 7 Biometra An Analytik Jena Company 2 Safety and Warning Notices 2 1 Definition of Symbols Symbol Definition Caution Refer to instruction manual Danger High voltage Fragile Danger Hot surface 2 2 General Safety Instructions Please read this manual carefully before starting operation the hybridisation ovens General safety precautions for laboratory work must be observed when working with the instrument ANA AA AA AA AA AA AA Parts in the oven and parts of the housing may be heated to up to 85 C Avoid any contact especially with metal parts hybridization bottles and solutions at high temperatures in the bottles Danger of burning Hybridization bottles containing hot solutions must not be opened or handled without safety protection Bottles containing hybridization solution must not be transferred rapidly from high to low temperature or vice verse Danger of explosion When opening hybridization bottles at high temperatures hot steam will escape Danger of burning and contamination Due to the sealing and heat insulation of the incubation chambers cooling down takes quite some time with doors closed Danger of burning after too short cooling times Before unscrewing parts to open the
8. d with easy to clean stainless steel 1 2 3 Shaking platform The ovens OV3 and OV5 are equipped with an additional platform that shakes with 1 to 10 rpm and may be used for different kind of applications 1 3 Technical specifications 1 3 1 Temperature Digital temperature setting Ambient temperature to 85 C 0 1 C steps Regulation range Ambient temperature 10 C to 85 C Digital display of actual temperature Ambient temperature to 85 0 C 0 1 C Heating up from 20 to 60 C 15 min Temperature varieties inside the chamber 1 0 C Note In the DUO Therm Hybridisation oven in case of using both chambers at the same time the minimal regulation temperature in the chamber used with the lower temperature may be shifted to a higher temperature 1 3 2 Rotor Capacity e 6 large hybridisation bottles 300 x 35 mm or Instruction Manual Hybridisation Ovens 10 2009 5 Biometra An Analytik Jena Company e 12 medium hybridisation bottles 150 x 35 mm or e 12 small hybridisation bottles 100 x 35 mm 1 3 3 Rotor speed Continuously adjustable about 1 to 9 5 rpm Shaking speed Mini Shaking Hybridisation oven OV3 and DUO Therm Hybridisation oven OV5 about 10 to 160 times back and forth 1 3 4 Electrical connection values Power supply 230 Volts 50Hz 052 000 100 200 300 115 Volts 50 60 Hz 052 090 190 290 390 maximum power consumption 0 63 kW OV5 0
9. e instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused Instruction Manual Hybridisation Ovens 10 2009 21 10 Subject index Accessories Auto Restart Declaration of Conformity Decontamination Certificate Disposal Hybridisation bottles Operating Operating elements 22 Biometra An Analytik Jena Company Rotor speed 6 13 Safety and Warning Notices 8 Safety precautions 8 Service 16 Shaking frequency 13 Shaking platform 5 Technical specifications 5 Warranty 21 Instruction Manual Hybridisation Ovens 10 2009
10. e 5 1 3 1 Temperaturen 5 1 3 2 erte O 5 1 3 3 PUCHO SPC RH 6 1 3 4 Electrical connection E 6 1 3 5 DIMENSIONS EE 6 1 amp Legal NOTES EET 6 1 4 1 EE ee 6 1 4 2 OF lr E 7 1 4 3 Meaning or the Insttuclons ae ne Sed ete pati o eR Hehe dua tance ENERO tase 7 2 Safety and Warning Notices ieiuna nan inu ra na IE ran Sas e inan ks costecancccsvacshsstecesciesectcs 8 2 1 Definition of Ssymb lsaun sn ee 8 2 2 General Safety Instructions nee deed up t npa vitta ia ecidu gege 8 Xr ogcun Me e r 10 3d Content of CS T ENSE 10 3 2 Unpack and Check TETTE EU om 10 3 3 Installation el 10 34 Operating elements 11 o one nasaan akaa Eear aaaeeeaa aaa aK See eer ne nee era eee eee re 12 Nei Le ON WEE 12 4 2 Display of the set termperature cccececcccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneeeeesessseeneeseneeeeeees 12 43 selling the temperdatule ui ete tesa eee HER pe odiis taa u e ter EE E S UEBR PE TD PME So UU Eneee 12 4 4 Set rotor speed and shaking frequency sssssssseeseseeeeeneeenene 13 4 5 Inserting ihe hybridisation bottles uses ae para dont sabe iunc rivedere fetus 13 4 6 Removing the hybridisation bottles rre reete x bx ken c o nc Eu RR 13 47 SUIOIMGSI E EE 14 4 8 Working at ambient temperature ea 14 DH tele121 1 70 oo 15 RE eege eege
11. f radioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details 18 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company 7 Note for the disposal of electric electronic waste for disposal of electric electronic Note waste fur die Entsorgung von Hinweis Elektroaltger ten du traitement des d chets des appareils lectrique lectronique This symbol the crossed out wheelie bin means that this product should be brought to the return and or separate systems available to end users according to yours country regulations when this product has reached the end of its lifetime Renseignement For details please contact your local distributor This symbol applies only to the countries within the EEA EEA European Economics Area comprising all EU members plus Norway Iceland and Liechtenstein Dieses Symbol die durchgestrichene Abfalltonne bedeutet dass dieses Produkt von der Firma Biometra fur eine kostenlose Entsorgung zuruckgenommen wird Dies gilt nur fur Gerate die innerhalb Deutschlands gekauft worden sind Kontaktieren Sie fur die Entsorgung bitte die Biometra Service Abteilung AuBerhalb Deutschlands wenden Sie sich bitte an den lokalen Handler Dieses Symbol gilt nur in Staaten des EWR EWR Europ ischer Wirtschaftsraum umfasst die EU Mit
12. gliedsstaaten sowie Norwegen Island und Liechtenstein Cet symbol conteneur d chets barr d une croix signifie que le produit en fin de vie doit tre retourn un des systemes de collecte mis la disposition des utilisateurs finaux en cons quence des r gulations par la loi de votre pays Pour des information additionel nous Vous demandons de contacter votre distributeur Cet symbole s pplique uniquement aux pays de I EEE EEE Espace conomique europ en qui regroupe les tats membres de l UE et la Norv ge Islande et le Liechtenstein Instruction Manual Hybridisation Ovens 10 2009 19 Biometra An Analytik Jena Company 8 EU Konformitatserklarung EU Declaration of Conformity G ttingen den 24 08 2004 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within certain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertraglichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erklaren wir daB folgende Hybridisierungsofen Herewith we declare that the following Hybridization Ovens Typen Mini Hybridisierungsofen OV1 Mini Hybridisierungsofen OV2 Mini Shaking types Hybridisierungsofen OV3 DUO Therm Hybridisierungsofen OV5 Mini Hybridization Oven OV1 Mini H
13. hen working with infectious pathogenic or radioactive material Ask the responsible local safety inspector for details The hybridisation oven must not be used with explosive flammable or volatile liquids Do not move the rotor by hand To change the rotor position close the hybridization oven door and switch the rotor on Be careful when loading the oven with sharp or hard items Do not abut against the glass door Danger of breakage of glass and implosion Do not use abrasives to clean the instrument For transports always use the original Biometra box gt gt gt gt ppp PPPPPPPP PL Instruction Manual Hybridisation Ovens 10 2009 9 Biometra An Analytik Jena Company 3 Installation 3 1 Content of delivery Hybridisation oven Diptray shaking platform Rotor wide necked hybridization bottles 300 x 35 mm with meshes 5 pcs 230 x 230 mm Power cable Instruction manual D OD OO D Please keep the original packaging material for return shipment in case of servicing 3 2 Unpack and Check Unpack and carefully examine the instrument Report any damage to Biometra Do not attempt to operate this device if physical damage is present Please keep the original packing material for return shipment in case of service issues N Attention A Please fill out and send back the warranty registration card This is important for you to claim full warranty 3 3 Installation Conditions e Place the
14. ike to send the unit back to us please read the following return instructions in chapter 6 1 6 1 Instructions for return shipment In case of an instrument failure that cannot be fixed please proceed as follows e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 50881 10 12 Fax 49 551 50881 11 e mail service biometra com e Please contact our service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e Important Carefully clean all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device e Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see section 6 2 must be attached to the outside of the packaging e Use the original packing material If not available contact Biometra or your local distributor e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains the foll
15. owing a Senders name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible 16 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company 6 2 Equipment Decontamination Certificate To enable us to comply with german law i e 71 StrliSchV 17 GefStoffV and 19 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Method of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER Instruction Manual Hybridisation Ovens 10 2009 17 Biometra An Analytik Jena Company General Information for Decontamination Please contact your responsible health amp safety officer for details Use o
16. rmed by the SET key SV is not blinking any more Immediately after confirmation the controller will regulate to the new temperature Note The set temperature should be at least 10 C above the ambient temperature Otherwise in continuous use the set temperature might be exceeded 4 4 Set rotor speed and shaking frequency At the left limit of the knob for setting the rotor speed is the function to turn the rotor on or off To switch the rotor drive off turn the knob counter clock wise over the left limit The ovens containing an integrated shaker Mini Shaking Hybridization oven OV3 and DUO Thermo Hybridization oven OV5 provide an additional turning knob marked Shaker for the shaking speed this one as well has an on off switch at the left stop In the OV 3 it can be used together with the rotor in case the vessels to be shaken are not higher than16 5 cm The shaking speed can be set from about 10 to 160 per minute 4 5 Inserting the hybridisation bottles The rotor axis is connected to the drive by a coupler it fits in two positions After positioning the rotor in the coupler on the left side it must be slightly pushed to the left and afterwards downwards into the holder on the right side By slightly lifting the rotor on the right side it can be taken out To load the bottles the rotor should be taken out of the oven This way the bottles can be pushed into the clamps from the sides Pushing the bottles into the rotor from the front
17. side may dilate the clamps after some time Clamps can be exchanged easily and are available as spare parts To additionally allow the hybridization solution to move back and forth the bottles can be placed slightly inclined Therefore the left clamp disk on the rotor can be dismantled by a knurled screw on the axis on the disks inner side The left clamp disk can be fixed in any position by this knurled screw 4 6 Removing the hybridisation bottles To remove the bottles take out the rotor from the instrument see chapter 4 5 and pull the bottles out of the spring clamps from the side Pulling out the bottles off the rotor from the front side may dilate the clamps after some time Hybridization bottles containing hot solutions must not be opened or handled without safety protection Bottles containing hybridization A A solution must not be transferred rapidly from high to low temperature or vice verse Danger of explosion When opening hybridization bottles at high temperatures hot steam will escape Danger of burning and contamination Parts in the oven and parts of the housing may be heated to up to 85 C Avoid any contact especially with metal parts hybridization bottles and solutions at high temperatures in the bottles Danger of burning In case the hybridization oven is not used for a longer period please remove all liquids from the bottles and from inside the chambers Remove the rotor from the oven and also remove all bottles
18. ubated do not place them beside each other but leave one clamp empty before placing the next If just one bottle is used an empty bottle should be placed opposite to it e Make sure that the ventilation slots on the bottom and the rear are not obstructed Make sure that there is no object underneath the instrument that may block the ventilation slots at the bottom e g a piece of paper etc e There must be sufficient distance between the ventilation slots on the rear side of the hybridisation oven and a wall or another instrument min 10 cm Ensure that both the rear and bottom ventilation slits of the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument 3 4 Operating elements Key function Description switch Green Power Mains switch for on off set keys VA set temperature value u Setting and storing of desired Key SET Set temperature temperature Turning knob rotor Set rotor speed LCD display O Actual temperature red set temperature green Instruction Manual Hybridisation Ovens 10 2009 11 Biometra An Analytik Jena Company 4 Operating 4 1 Switching on After switching on the oven Power switch the display will show the actual temperature in the oven and the last set temperature value Heating up to the set temperature starts immediately The rotor starts turning immediately after switching on as
19. well in case the turning knob for the rotor speed is not at the left stop The shaking platform starts moving with the speed set too When starting the shaking platform a slow speed should be chosen The speed then can be regulated up adequate to the vessels placed on the shaking platform When placing the shaking platform into the oven care must be taken the drive slips into the central guide rail The upper red display shows the actual temperature inside the oven chamber the lower green display shows the temperature value set see chapter 4 2 In continuous use after heating up both displays will show the same temperature 4 2 Display of the set temperature The set temperature is shown in the lower green display Actual temperature red Set temperature green B b4 ES ES 4 3 Setting the temperature The temperature is set by pressing the V and A keys on the controller Increase A or decrease V the value Therefore first the SET key is pressed until SV starts blinking left above the set value Now one of the V A keys is pressed several times or permanently With a key kept pressed the set value increases decreases slowly at first The longer the key is held the faster the set temperature value will change This allows the user to easily perform large temperature changes 12 Instruction Manual Hybridisation Ovens 10 2009 Biometra An Analytik Jena Company The new temperature value set must be confi
20. ybridization Oven OV2 Mini Shaking Hybridization Oven OV3 DUO Therm Hybridization Oven OV5 Best Nr 052 000 052 090 052 100 052 190 052 200 052 290 052 300 052 390 Order No den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG und der EC low voltage directive 73 23 EEC and the EG Richtlinie ber die elektromagnetische Vertraglichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 55011 1998 A1 1999 A2 2000 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 EN 61000 6 1 2001 EN 61010 1 2004 Dr J rgen Otte Quality Manager 20 Instruction Manual Hybridisation Ovens 10 2009 Formular 006 Ver 03 Biometra An Analytik Jena Company 9 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the following conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with th
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