Operator`s Manual
Contents
1. or authorized for use in applications or as system components intended to support or sustain human life as a clinical medical device for humans or for any application in which the failure of the product could create a situation where personal injury or death may occur All brand and product names used in this manual are the trademarks of their respective owners NEXT ADVANCE INC DOES NOT GUARANTEE THE INTEGRITY OF THE TUBES USED IN THE BULLET BLENDER TUBES THAT ARE NOT RECOMMENDED BY THIS MANUAL MAY CRACK OR OPEN IF USED IN THE BULLET 7 ADVANCE Operator s Manual for the Bullet Blender BLENDER NEXT ADVANCE INC OPTIMIZES THE BULLET BLENDER TO SPECIFIC TUBE TYPES AND BRANDS AND CANNOT GUARANTEE THE SAFE USE OF ALL TUBES BEING SOLD ON THE MARKET CONTACT INFO Next Advance Inc Averill Park NY USA Telephone 518 674 3510 www nextadvance com Email info nextadvance com support nextadvance com sales nextadvance com NEXT gt gt ADVANCE OO2. 9 in wide x 21 5 cm 8 5 in high Weight 4 to 5 kg 8 5 to 11 Ibs depending upon model Power Requirement BBX24 and BBX24B models 24 VDC 0 75 Amp BBY24M BBY5E and BBY5M models 24 VDC 2 5 Amp Capacity 24 of 1 5 2 0 mL poly propylene high quality e g Eppendorf snap cap tubes 24 of 1 5 mL RINO screwcap tubes 12 of 5 mL Eppendorf tubes or 12 of 5 mL Axygen 5 mL transport tubes depending upon model Nn ADVANCE Operator s Manual for the Bullet Blender Relative Humidity 5 90 non condensing Operating Temperature 4 40 C Altitude lt 2000m Storage Temperature 40 to 50 C Meets requirements CE models only WARRANTY Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product Within these time periods Next Advance will replace or repair without charge to the original purchaser any part which is defective Bullet Blender warranty Two years The warranty is void if the Product is defective due to product accident product modification exposure to radiation other than for sterilization connection to an improper electrical supply lack of proper maintenance contamination improper installation or misuse If the product is used in a manner not specified by _ the manufacturer the protection provided by the equipment may be impaired The warranty shall also not apply to defects arising fr
3. Operator s Manual The Bullet Blender Models Bullet Blender Bullet Blender Storm Bullet Blender Blue Bullet Blender 5 Storm and Bullet Blender 5 E Congratulations Congratulations on your purchase of a Bullet Blender by Next Advance Inc for lysing disrupting and homogenizing your samples Please read this operator s manual which explains proper operation of the instrument This manual is posted on our website www nextadvance com Click the SUPPORT button on the left hand menu bar and then on the appropriate link to the manual We re confident that your Bullet Blender will become an essential tool in your laboratory and we wish you success with your work Contents Parts of the Bullet Blender 2 SLS L o EE A E bees senducubesendes Operation crises snanensndereaeaceiedewatiaucaonsesnes Protocols and Sample Settings 4 CVC AMIS e 5 Troubleshooting cccccccccccssssseeesseees 5 SUPPO esea e 5 Specifica ONS esseiste irenda 5 M TA Voren ON 6 Warnings and Cautions 00008 6 Dis laMe sof sverinastidnsnminancramrerneaseaconoaets T Contact Info NEXT gt ADVANCE Operator s Manual for the Bullet Blender Parts of the Bullet Blender Cover Operator Panel SYMBOLS USED ON THE BBX AND BBY MODEL BULLET BLENDERS Start Operation A Caution Follow the Instructions in the Operator s Manual C This product complies wi
4. an two thirds of the way after the addition of all contents sample beads and buffer This is because the mechanics of homogenization require empty space in the tube For 5 mL tubes the recommended maximum sample mass is 1 g of organ tissue or mL of plant tissue or pelleted cell culture per tube Do not operate with more than a total of 3 mL combined buffer sample and beads per tube Cutting the tissue into smaller pieces will generally yield better results Tissue with a high aspect ratio ong thin strips will homogenize better than tissue that 1s round or cubic Do not operate the Bullet Blender using the same tubes for longer than 15 minutes Protocols for many types of samples are posted on our website http protocols nextadvance com P ADVANCE Operator s Manual for the Bullet Blender NOTES The Bullet Blender Storm 5 is designed to work with 5 mL Axygen brand tubes only At high speed settings there may be some flaking of the tubes This is a normal side effect of homogenization The higher speed enables homogenization of tougher tissue CLEANING If you wish to clean your Bullet Blender clean the outside of the unit only with mild soap water and a soft cloth Under normal conditions the Bullet Blender should never need to be disassembled for cleaning In the case of a large spill unplug the instrument remove the sample tube plate with a 1 8 hex wrench wipe out the spill using standar
5. bes only ON ADVANCE Operator s Manual for the Bullet Blender No user serviceable parts are inside of the instrument For indoor use only Pollution Degree 2 per EN 61010 1 Overvoltage Category II per EN 61010 l Enclosure Protection Not Protected Against the Ingress of Moisture Sound Pressure Level up to 90dBA for BBX24 models up to 100dBA for BBY24M BBYS5E and BBX5MB models Use hearing protective devices that reduce exposure to below 85 dBA during prolonged exposure Do not immerse in liquid Before touching the Bullet Blender touch a bare metal surface to discharge static electricity NEXT gt gt DISCLAIMER Next Advance Inc makes no representations or warranties expressed statutory or implied regarding the fitness or merchantability of this product for any particular purpose In no event shall Next Advance be liable for incidental or consequential damages Next Advance Inc is not liable for any damages including but not limited to lost profits lost savings or other incidental or consequential damages arising from ownership or use of this product or for any delay in the performance of its obligations under the warranty due to causes beyond its control Next Advance Inc also reserves the right to make any improvements or modifications to the product described in this manual at any time without notice of these changes Next Advance Inc products are not designed intended
6. d laboratory safety precautions and replace the sample tube plate Do not touch or tamper with the electronics Contact tech support for a detailed protocol support nextadvance com TROUBLESHOOTING In addition to the tips given below thorough list of troubleshooting tips 1s at http bbtroubleshooting nextadvance com If the Bullet Blender doesn t start the plug of the power supply cord may not be in a live wall outlet or the power supply connector may not be fully inserted in the socket on the back of the Bullet Blender If the unit stops working turn the system off for 15 minutes to allow the electronics to reset If the Bullet NEXT gt gt Blender does not turn on after this period contact customer service If the caps on snap cap tubes pop open or the caps on screw cap tubes loosen make sure that the interface regions or screw threads between the lids and the caps is dry when you close the caps or screw them on so that there is enough friction for the caps to remain tight Using recommended types of tubes will minimize cap failure SUPPORT FAQs protocols and other helpful information are available on our website http www nextadvance com Click on the Bullet Blender then on the appropriate link If you cannot find an answer there please contact customer service by email at support nextadvance com or by telephone at 1 518 674 3510 or 800 738 1681 SPECIFICATIONS Size 28 cm 11 in deep x 23 cm
7. l will light up After the second minute the second LED behind the number 2 will light up and so on At the settings Shown above after 4 minutes the Bullet Blender will stop In the Blue and Storm modles the words Air Cooling will light up in blue during operation and for about one half minute afterward as the fan continues to operate Note that the Bullet Blender Storm models have additional time settings for 15 and 30 second pulses In addition the Bullet Blender Storm has an additional more powerful speed setting of 12 PROTOCOLS AND SAMPLE SETTINGS The following ratio should be used as a guideline for determining the amount of beads and buffer to use given a certain sample size 1 volume mass of tissue volume of beads 2 volumes of buffer For more specific information regarding the use of various beads as NEXT gt D well as specific protocol information please refer to our website www nextadvance com As the tissue amount becomes smaller the above recommended ratio may differ due to the limitations of handling small volumes With microcentrifuge tubes we recommend using a minimum of 25 uL of buffer regardless of your sample size For the 5 mL tubes we recommend a minimum volume of 100 UL With microcentrifuge tubes the recommended maximum sample mass is 300 mg of organ tissue or 300 uL of plant tissue or pelleted cell culture per tube in the Bullet Blender The tube should not be filled more th
8. om fire flood lightning or other conditions unrelated to correct operation of the Product Next Advance s liability is limited at the company s election to 1 refund of the original purchaser s purchase price for the Product 2 repair of the Product or 3 replacement of the Product or defective parts Evidence of NEXT gt gt purchase by the original purchaser is required Next Advance may also request documentation of proper maintenance if applicable Next Advance makes no other warranty expressed or implied with respect to its Products NEXT ADVANCE MAKES NO WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR THEIR SUITABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE Next Advance shall not be liable for indirect special incidental or consequential damages of any nature Any recovery for any claim shall be limited to the original purchase price for the product OPERATOR S RESPONSIBILITY Provide proof of purchase and provide normal care and maintenance WARNINGS AND CAUTIONS Read the users manual _ before operating Do not open lid when Bullet Blender is in use Do not insert fingers or objects other than recommended tubes into sample tube holes Use caution when closing Bullet Blender lid do not close on fingers When working with hazardous or pathogenic samples operate the Bullet Blender in a biosafety cabinet or other standard laboratory safety enclosure Use recommended tu
9. th European Low Voltage and EMC Directives Please dispose the test tubes and the BBX or BBY Bullet Blender in accordance with local regulations 1 al oe i F a NO NEXT gt gt ADVANCE Operator s Manual for the Bullet Blender SETUP Place the Bullet Blender on a stable level lab bench Plug the cord from the AC DC power adapter into the 24V socket on the back of the Bullet Blender and then insert the plug into a wall outlet You should see lights on the time setting flash for several seconds indicating that the unit is receiving power It is now set up OPERATION To begin using your BBX or BBY series Bullet Blender lift open the cover and insert the appropriate tubes For BBX24 models BBX24 and BBX24B place up to twenty four closed 1 5 mL to 2 0 mL polypropylene high quality snap cap e g Eppendorf Safe Lock microcentrifuge tubes containing samples into the sample plate and close the cover When using 2 0 mL tubes we recommend using at least two tubes per homogenization cycle In the Bullet Blender 5 Storm BBY5M load up to twelve 5 mL Axygen brand screw cap tubes In the Blender 5 E BBY5E load up to twelve 5 mL Eppendorf tubes For the Bullet Blender Storm BB Y24M use up to 24 Next Advance RINO screw cap tubes or 1 5 mL Eppendorf Safe Lock snap cap tubes In order to use these tubes the Bullet Blender must be fitted with the appropriate gasket corresponding to the tube
10. type Each gasket is clearly labeled NEXT gt gt RINO or Eppendorf The gasket is removed by unscrewing the three screws with a Phillips head screw driver and lifting the gasket off of the lid Attach the proper gasket by aligning the holes in the gasket with the holes in the lid and inserting the screws Make sure that the gasket is screwed on securely or else homogenization efficiency may be affected Do not over tighten the screws Only RINO screw cap tubes are recommended for use in the Bullet Blender outfitted with the RINO gasket other screw cap tubes may break or result in sub optimal homogenization The snap caps on the microcentrifuge tubes must be closed securely before using in the Bullet Blender The threads on all screw cap tubes must be dry and the caps must be screwed on very tightly before using in the Bullet Blender Do not operate with the cover open There will be excess noise your samples will not be properly processed and the tubes may be knocked entirely out of the instrument potentially causing injury If you turn the minutes knob to 0 the instrument will stop 09 ADVANCE Operator s Manual for the Bullet Blender EXAMPLE The figure above shows the Bullet Blender set to run for 4 minutes at a speed of 8 after running for 2 minutes To operate press the START button After the first minute the LED behind the number on the minutes dia
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