Hematocrit Rotor User Manual
Contents
1. V V z NEOPRENE z v a SILICONE RUBBER lt A HA O Chromic Acid 50 C C A c C C AN Cresol Mixture Cyclohexane Deoxycholate Distilled Water Dextran Diethyl Ether NA A cc AA ND N C N Diethyl Ketone Diethylpyrocarbonate Dimethylsulfoxide Dioxane A A A A A c c z POLYCARBONATE c c z 9 9 Z lt lt a POLVINYL CHLORIDE RULONA TEFLON Z Ferric Chloride co Acetic Acid Glacial 40 Hematocrit Rotor o cz oo lt S 9 ALUMINUM c BUNAN Z vv dv v ov DELRIN c aj aj NYLON i z z V z c c POLYALLOMER2. 8 Rotor Specifications ninna 9 Thermo Scientific Heraeus Megafuge 8 Centrifuge 10 Thermo Scientific Sorvall ST 8 Centrifuge 11 Thermo Scientific SL 8 Centrifuge aa jae eke See EE REOR ee i 13 ACCESSORIES ran aa 15 Thermo Scientific Auto Lock Rotor Exchange 17 Botor Installation 5222254 ea bora 18 Removing the ROC siii Lele 19 R t r rni MEER u m 21 e e d p a p ok UE pla 22 Filling ihe Hematocrit Capillaries 25 bebe 22 Rotor Temperature Rance d ea eo 22 Rotor Loading ced crte mies een ER b A 22 Maximum Loading siriaca cord epe w od apo esita 24 Using the Reading Graph Loue ce edo seas s eX REC ai eee Ree durs 24 Rotor Lifetime ireland 25 Maintenance and Care ia ia ai ira 21 Cleaning oo eoe 28 Replace Sedo 28 How To Deal With Broken Hematocrit Capillary Tubes 29 CAME iii ee 29 cn 31 Dec ntaminati n eres cessi t t Hm ERIS S ie roi 32 qe PETI 33 Thermo Fisher Scientific 33 Thermo Scientific Hematocrit Rotor 5 Contents ie LI eT E Deeds vee nae 35 Chemical Compatibility 39 Con
3. Visit us online to register your warranty www thermoscientific com labwarranty 2013 Thermo Fisher Scientific Inc All rights reserved HERAEUS is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific Delrin TEFLON and Viton are registered trademarks of DuPont Noryl is a registered trademark of SABIC POLYCLEAR is a registered trademark of Hongye CO Ltd Hypaque is a registered trademark of Amersham Health As RULON A and Tygon are registered trademarks of Saint Gobain Performance Plastics Alconox is a registered trademark of Alconox Ficoll is a registered trademark of GE Healthcare Haemo Sol is a registered trademark of Haemo Sol Triton X 100 is a registered trademark of Sigma Aldrich Co LLC All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc Manufacturer Thermo Electron LED GmbH Robert Bosch Strafe 1 D 63505 Langenselbold Germany The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document
4. Figure 2 Handling of Auto Lock Thermo Scientific Hematocrit Rotor 19 4 Thermo Scientific Auto Lock Rotor Exchange Removing the Rotor 20 Hematocrit Rotor Thermo Scientific 5 Rotor Loading Rotor Loading Thermo Scientific Hematocrit Rotor 21 5 Rotor Loading Before a Run Before a Run 1 Please read and observe the safety instructions contained in these operating instructions and in the instructions for use 2 Check the rotor and all accessory parts for damages such as cracks scratches or traces of corrosion 3 Check the rotor chamber the centrifuge spindle and the Auto Lock 4 Check the rotor suitability using the Chemical Compatibility Chart on page 39 Filling the Hematocrit Capillaries Note Mind the lifetime of the capillaries 1 Fill the hematocrit capillary 760000923 with a blood column of approximately 65 mm Make sure that the second opening remains dry 2 Close the dry end of the hematocrit capillary with sealing putty 75000964 To do this push the hematocrit capillary vertically into the sealing putty until its rim touches the sealing putty s plate Tilt it slightly and pull the hematocrit capillary carefully out of the putty Make sure that the capillaries are well sealed with the sealing putty WARNING The rotor cannot be used for aerosol tight applications The O ring on the rotor lock has the function to secure the rotor lid when screwed on The external lid lip cannot be
5. z c z c z ajajajaj POLYRTHYLENE z z ajal z ajajaj POLYPROPYLENE NM aj zl SM coo o aj aj TITANIUM al oo o Thermo Scientific B Chemical Compatibility Chart DS lt gt gt a E REG Sys cc lt Lu lt 2 LU di s HB e DE E eo gt 0 lt 555 Se ie BE avs seu 57 3523 pB So ome E Ce CHEMICAL Seam oe ee Acetic Acid 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SM Acetic Acid 60 S SU US 5 U SMS UUMUS 5 MS MS MUS MU Ethyl Acetate MMUUS SMMS SUS U MU U 5 5 UU 5 MMS U Ethyl Alcohol 50 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SMS MU Ethyl Alcohol 95 5 5 5 5 SMS 5 5 5 5 5 5 5 5 5 SUS MU Ethylene Dichloride S UU SMO 5 UUUUUUU US U 5 5 Ethylene Glycol 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 MS Ethylene Oxide Vapor S U U 6 U 6 SM 5 5 5 US US 5 SU Ficoll Hypaque MS 5 5 5 5 5 5 5 5 5 5 5 6 5 6 5 5 5 5 5 5 Hydrofluoric Acid 10
6. 2 c cin DA A A A A A gt 1 1 0 0 cc N N Dimethylformamide 42 Hematocrit Rotor co co co co co c z z co co co Thermo Scientific B Chemical Compatibility Chart n z lt gt gt a E 8 lt EE E Sys cc 2 2 lt Lu lt 2 es T 5 o 2s p ES u ESBg lt GS m G e cc uj ct 7 9 C z uii cruitmtr lt LZ 9 pu BSS ES ES m m o 2 O0 oloez SOrHR tot Sos BSS ESS ESP SRR RSS CHEMICAL Jaen e m cL RUSSIE BG ZI Sodium Borate 5 5 5 5 65 65 65 65 565 5 65 5 6 65 565 6 6 6 6 6 5 5 5 Sodium Bromide US 5 5 5 5 65 65 565 65 6 65 65 565 565 6 6 6 6 6 5 5 5 Sodium Carbonate 2 MUS 5 5 5 5 5 5 5 5 5 5 5 US 5 5 5 5 5 5 5 5 5 5 Sodium Dodecyl Sulfate 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 5 6 5 5 6 5 5 5 5 E MS S MU S 5 5 5 5 5 5 SMS 5 5 MU SMS Sodium lodide MS 5 5 5 5 5 5 5 5 5 5 5 65 6 6 6 6 6 5 5 5 Sodium Nitrate 5 5 5
7. POLYPROPYLENE POLYSULFONE co N POLYVINYL CHLORIDE RULON A TEFLON SILICONE RUBBER c STAINLESS STEEL TITANIUM gt TYGON c VITON co Potassium Hydroxide conc 4 co c c Potassium Permanganate co co co co co N co co co c co Calcium Chloride co Calcium Hypochlorite Kerosene z Sodium Chloride 1096 cn zio Sodium Chloride sat d Carbon Tetrachloride ND o Zn o z Aqua Regia nA Nn 1 1 1 Solution 555 20 Magnesium Chloride co Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Methyl Ethyl Ketone V nm cy C c Metrizamide z c z nA TNA gt Zn zio vv cl A na na wn c Lactic Acid 100 Lactic Acid 20 lt oo N Butyl Alcohol oo N Butyl Phthalate un AN lt cu z c
8. 5 5 5 US U Ammonium Acetate 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Ammonium Carbonate MS US 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SMS 5 5 UUSUSSMSSSSS SUMSSSSSSSSSMS UUSUSUMSSSSSUSUMSSSSSSSSSMS Hydroxide UUUUSUMS S SUSUUSSS MSSSS U conc Ammonium Phosphate U 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Ammonium Sulfate UMS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 US SU Alcohol S 5 5 MS 5 5 5 M U 5 Aniline S SU US US MS UUUUUUU 5 MUUS 5 5 5 US U MS S S SMS S 5 MMS 8 5 55 SMS do U MU MMS 56 0500555555 5 5 10 Thermo Scientific Hematocrit Rotor 39 B Chemical Compatibility Chart CHEMICAL Barium Salts MATERIAL CELLULOSE ACETATE BUTYRATE POLYTHERMIDE Benzene gt c ANODIC COATING for ALUMINIUM a POLYURETHANE ROTOR PAINT c COMPOSITE Carbon Fiber Epoxy GLASS NORYL POLYSULFONE c STAINLESS STEEL v TYGON Benzyl Alcohol Boric Acid co z ETHYLENE PROPYLENE v PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYESTER GLASS THERMOSET Cesium Acetate Cesium Bromide Cesium Chloride Cesium Formate Cesium lodide Cesium Sulfate Chloroform Chromic Acid 10 NA A A NA A
9. UUUM U UUS SM 5 5 5 SMS UUU Hydrofluoric Acid 50 U U U U U UUUUS UUUS 5 MMS U U U M UUUU UUM UMUUMUUU S USUUU conc Formaldehyde 40 MMMS 5 5 SMS 5 5 5 5 5 5 5 5 8 SSMS MU Glutaraldehyde 5 5 5 5 6 5 5 5 5 5 8 5 5 5 6 6 6 6 Glycerol MS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Ui a a 2 9 gs ei Hydrochloride Haemo Sol SSS 6 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SUS UMUS SUS SMS US 5 5 Isobutyl Alcohol MU SS U 5 5 SMS 5 5 5 5 5 6 5 Isopropyl Alcohol MMM 5 5 5 5 5 5 SUS UMS 5 5 5 5 5 5 5 lodoacetic Acid SSM 5 5 5 SMS SMS 5 MS 5 5 5 5 MS 5 MM Potassium Bromide USS 5 5 5 5 6 5 5 5 5 5 6 5 5 6 5 55 5 5 5 Potassium Carbonate MUS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Potassium Chloride USS SS 5 5 5 56 5 5 5 5 5 5 5 6 5 5 5 5 5 SS Thermo Scientific Hematocrit Rotor 41 B Chemical Compatibility Chart CHEMICAL Potassium Hydroxide 5 MATERIAL C ALUMINUM ANODIC COATING for ALUMINIUM BUNAN CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT gt COMPOSITE Carbon Fiber Epoxy DELRIN co ETHYLENE PROPYLENE v GLASS v NEOPRENE v NORYL NYLON POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER co POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHERMIDE co co POLYRTHYLENE 2
10. g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at N max 16810 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK Thermo Scientific Hematocrit Rotor 3 Accessories Accessories Article No Capillaries 76000923 Sealing Kit 75000964 Rotor Seal Replacement 75003437 Replacement Linear Scale Reading Graph 76000938 Thermo Scientific Hematocrit Rotor 15 4 Thermo Scientific Auto Lock Rotor Exchange Thermo Scientific Auto Lock Rotor Exchange Contents Rotor Installation on page 18 e Removing the Rotor on page 19 Thermo Scientific Hematocrit Rotor 17 4 Thermo Scientific Auto Lock Rotor Exchange Rotor Installation Rotor Installation CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge This rotor is equipped with an Auto Lock system This system is used to automatically lock the rotor to the centrifuge spindle The rotor does not have to be bolted onto the centrifuge spindle Proceed as follows 1 Open the lid of the centrifuge and if necessary remove any dust foreign objects or residue from the chamber Auto Lock and O ring must be clean and undamaged Auto Lock O ring Figure 1
11. Auto Lock 2 Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle The rotor clicks automatically into place CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with a small amount of pressure 3 Check if the rotor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle WARNING Ifthe rotor cannot be properly locked in place after several attempts then the Auto Lock is defective and you are not permitted to operate the rotor Check for any damage to the rotor Damaged rotors must not be used Keep the hub area clear of objects CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle 18 Hematocrit Rotor Thermo Scientific 4 Thermo Scientific Auto Lock Rotor Exchange Removing the Rotor WARNING sure to check all seals before starting any aerosol tight applications 4 Close the centrifuge door Removing the Rotor To remove the rotor proceed as follows 1 Open the centrifuge door 2 Grab the rotor handle with both hands and press against the green Auto Lock button At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this
12. C 3 9 lt SILICONE RUBBER Trisodium Phosphate Tris Buffer neutral pH Triton X 100 Urea oco V 2 z POLYALLOMER z nn z z z 2 z lt S a Ww POLYRTHYLENE V 2 2 POLPROPYLENE RULON A TEFLON z Wi TITANIUM vITON9 Hydrogen Peroxide 10 44 Hematocrit Rotor c co co c co z nn co co Thermo Scientific lt e 5 a Few gt 52 lt irr lt 2 E Q Z o gt wus 5 fc oc cm mj 8 gt Og 28 5 EX m c4 on 4 Sa Ss E a e CHEMICAL Hydrogen Peroxide 3 5 5 5 5 5 5 5 5 5 5 5 5 5 Xylene 55 US 5 5 MUS UUUUUUM Zinc Chloride 5 5 5 5 5 5 5 5 5 5 5 5 5
13. Zinc Sulfate USS 5 5 5 5 5 5 5 5 5 5 5 5 Citric Acid 10 MS SMS 5 5 5 5 5 5 5 5 5 5 Polyethyleneterephthalate Key S Satisfactory M suggest testing under actual conditions of use U Unsatisfactory not recommended vo v POLYTHERMIDE z POLYRTHYLENE Mm POLYPROPYLENE POLYSULFONE a POLYVINYL CHLORIDE E LL CO eo eons 6822 cnn 5 5 5 5 SUMS SS US 5 5 5 5 5 5 5 5 M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc No data available Because no organized chemical resistance data exists for materials under the stress of centrifugation when in doubt we recommend pretesting sample lots suggest testing using sample to avoid loss of Thermo Scientific valuable material Chemical resistance data is included only as a guide to product use Hematocrit Rotor vv TYGON vv VITON 45 Contact Thermo Scientific Country United States Canada C Contact Telephone number 1 866 984 3766 Latin America 1 866 984 3766 Austria 43 1801400 Belgium 32 53 73 42 41 France 33 2 2803 2180 Germany 0800 1 536 376 49 6184 90 6000 Italy 39 02 95059 552 Netherlands 31 76 579 55 55 Nordic Baltic Countries CIS 358 9 329 10200 Russia 7 812 703 42 15 Spain Portugal
14. applications their might be ice in the rotor chamber Let the ice melt and drain it off Clean the rotor chamber as described above 30 Hematocrit Rotor Thermo Scientific Disinfection 6 Maintenance and Care Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation Use The WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if need be a sprayer whenever possible so that all surfaces are covered evenly rotor chamber and the rotor should be treated preferably with a neutral disinfectant CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Observe the safety precautions and handling instructions for the cleaning agents used Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants Disinfect the rotor and accessories as follows 1 QN o N 10 Thermo Scientific Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the ro
15. required both in interest of patients and blood donors and to guarantee the comparability of research results The hematocrit value allows you to calculate the rate of erythrocytes in the blood This applies only if the Hematocrit Rotor is used with approved accessories and items listed in the items supplied This rotor should be operated by trained specialists only Hematocrit Rotor 7 1 Precautions Precautions In order to ensure safe operation of the Hematocrit Rotor the following general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and or cracks Do not touch the electronic components of the rotor and do not make any changes to the electronic or mechanical components e Use only with rotors which have been loaded properly Never overload the rotor Use only accessories which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have been approved for the speed or the RCF value of the rotor Observe the safety instructions Pay particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 1 2 This symbol refers to general hazards CAUTION means that materia
16. sealed Rotor Temperature Range CAUTION Operate the rotor in a temperature range between 9 C and 40 C only A pre tempering in a freezer below 9 C is not allowed Note The rotor can warm up in ventilated centrifuges Temperature above 45 C the samples can be damaged Let the rotor cool down between two runs 22 Hematocrit Rotor Thermo Scientific 5 Rotor Loading Duration Duration The duration depends on the RCF value For a clear separation the run should last at least 5 minutes The following values are suggested for reproducible results 13300 8 Rotor Loading 1 Place the hematocrit capillary with its closed end pointing outwards towards the rim of the rotor horizontally into the hematocrit rotor The hematocrit capillary must touch the sealing 7500 3437 The sealing protects the sensible capillaries from the hard rotor rim The capillaries can break during a run when the sealing is missing CAUTION Ensure that a balancing hematocrit capillary is placed opposite in order to avoid imbalance and possible damage If not all places are filled with hematocrit capillaries they must be placed with an opposite 2 Close the rotor lid by turning it clockwise CAUTION Damaged parts must be replaced immediately Thermo Scientific Hematocrit Rotor 23 5 Rotor Loading Maximum Loading Maximum Loading The rotor can run at high speeds The rotor design has sufficient reserve stability even when spi
17. 10 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK 10 Hematocrit Rotor Thermo Scientific 2 Rotor Specifications Thermo Scientific Sorvall ST 8 Centrifuge Thermo Scientific Sorvall ST 8 Centrifuge Table 3 Sorvall 230V 50 60Hz Centrifuge Sorvall ST 8 Catalog 75007201 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at N pax 16810 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK Table 4 Sorvall 120V 60Hz Centrifuge Sorvall ST8 Catalog 75007200 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at N max 16810 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Te
18. 22 95 1100 2 0 8 5 27 115 1200 2 0 8 5 32 137 1300 2 0 8 5 38 161 1400 2 0 8 5 44 186 1500 2 0 8 5 50 214 1600 2 0 8 5 57 243 1700 2 0 8 5 65 275 1800 2 0 8 5 72 308 1900 2 0 8 5 81 343 2000 2 0 8 5 89 380 2100 2 0 8 5 99 419 2200 2 0 8 5 108 460 2300 2 0 8 5 118 503 2400 2 0 8 5 129 547 2500 2 0 8 5 140 594 2600 2 0 8 5 151 642 2700 2 0 8 5 163 693 2800 2 0 8 5 175 745 2900 2 0 8 5 188 799 3000 2 0 8 5 201 855 3100 2 0 8 5 215 913 3200 2 0 8 5 229 973 3300 2 0 8 5 244 1035 3400 2 0 8 5 258 1099 Thermo Scientific Hematocrit Rotor 35 A RCF Values Speed rpm Rmin Rmax RCF Rmin RCF Rmax 3500 2 0 8 5 274 1164 3600 2 0 8 5 290 1232 3700 2 0 8 5 306 1301 3800 2 0 8 5 323 1372 3900 2 0 8 5 340 1445 4000 2 0 8 5 358 1520 4100 2 0 8 5 376 1597 4200 2 0 8 5 394 1676 4300 2 0 8 5 413 1757 4400 2 0 8 5 433 1840 4500 2 0 8 5 453 1924 4600 2 0 8 5 413 2011 4700 2 0 8 5 494 2099 4800 2 0 8 5 515 2189 4900 2 0 8 5 537 2282 5000 2 0 8 5 559 2376 5100 2 0 8 5 582 2472 5200 2 0 8 5 605 2570 5300 2 0 8 5 628 2669 5400 2 0 8 5 652 2771 5500 2 0 8 5 676 2875 5600 2 0 8 5 701 2980 5700 2 0 8 5 726 3088 5800 2 0 8 5 752 3197 5900 2 0 8 5 778 3308 6000 2 0 8 5 805 3421 6100 2 0 8 5 832 3536 6200 2 0 8 5 860 3653 6300 2 0 8 5 887 3772 6400 2 0 8 5 916 3892 6500 2 0 8 5 945 4015 6600 2 0 8 5 974 4140 6700 2 0 8 5 1004 4266 6800 2 0 8 5 1034 4394 6900 2 0
19. 34 93 223 09 18 Switzerland 41 44 454 12 22 UK Ireland 44 870 609 9203 China 86 21 6865 4588 86 10 8419 3588 India 91 22 6716 2200 Japan 81 45 453 9220 Other Asian Countries 852 2885 4613 Australia 61 39757 4300 New Zealand 64 9 980 6700 Countries not listed D Hematocrit Rotor 47 O Y Index A Accessories 15 Autoclaving 33 Auto Lock 17 Before a Run 22 C Chemical Compatibility Chart 39 Cleaning 29 Cleaning Intervals 28 Decontamination 32 F Filling the Hematocrit Capillaries 22 H Heraeus 10 How to deal with broken Hematocrit Capillary Tubes 29 Intended Use 7 Items Supplied 7 Maintenance and Care 27 Maximum Loading 24 Precautions 8 Preface 7 RCF Values 35 Removing the Rotor 19 Replace Sealing 28 Thermo Scientific Rotor Installation 18 Rotor Life time 25 Rotor Loading 21 22 Rotor Specifications 9 Rotor Temperature Range 22 5 Sorvall 11 T Thermo Fisher Scientific Service 33 U Using the Reading Graph 24 WEEE compliance 3 Hematocrit Rotor 49 Part of Thermo Fisher Scientific 3 SCIENTIFIC
20. 5 5 5 5 5 5 5 5 5 5 6 5 6 65 5 65 5 5 5 5 5 Sodium Sulfate USS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Sodium Sulfide 5 5 5 6 5 UU 65 6 6 M 5 Sodium Sulfite 5 5 5 5 5 5 5 5 5 5 5 5 SM 5 5 5 5 5 5 5 5 5 5 Nickel Salts US 5 5 5 5 6 5 565 5555 65 6 65 6 6 6 5 5 5 Oils Petroleum SSS SU 5 5 5 5 UUMS MUUS 5 5 US 5 5 Oils Other 5 5 6 MS 5 5 SUS 5 5 SUS 5 5 5 SSMS Oleic Acid S US 5 5 UUS US 5 5 5 5 5 5 5 5 5 5 MUS MM Oxalic Acid UUMS 5 5 US 5 5 5 5 US US 5 5 5 5 5 5 SUMS 5 Perchloric Acid 10 U U SUU SMM MUMS MM MS 5 S Perchloric Acid 70 U U U UU SU MU UMU UUMMUMS US Phenol 5 U SU SMM 5 UMUUS UMS MS U U SUMMMS Phenol 50 U SU SUM SU MU U U U U SU MU US U UU MS Phosphoric Acid 10 U U MS 5 5 5 5 5 SU 5 65 5 5 6 6 6 6 6 Phosphoric Acid conc U U MM US MS UUMMS 5 5 5 MS SS Physiologie Media 5 5 5 5 55 5 555 5 55 5 5 5 5 8 55 5 5 5 Serum Urine Picric Acid SSU SMS SSMS 5 5 5 US 5 5 5 US UMS MS Pyridine 50 U S U SUU 5 5 U MU US M SSUUUU Thermo Scientific Hematocrit Rotor B Chemical Compatibility Chart CHEMICAL Rubidium Bromide MATERIAL CELLULOSE ACETATE BUTYRATE ETHYLENE PROPYLENE POLYESTER GLASS THERMOSET POLYTHERMIDE Rubidium Chloride Sucrose Sucrose Alkaline co a a co POLYSULFONE Sulfosalicylic Acid PET POLYC
21. 8 5 1065 4524 7000 2 0 8 5 1096 4656 7100 2 0 8 5 1127 4790 36 Hematocrit Rotor Thermo Scientific RCF Values Speed rpm Rmin Rmax RCF Rmin RCF Rmax 7200 2 0 8 5 1159 4926 7300 2 0 8 5 1192 5064 7400 2 0 8 5 1224 5204 7500 2 0 8 5 1258 5345 7600 2 0 8 5 1292 5489 7700 2 0 8 5 1326 5634 7800 2 0 8 5 1360 5782 7900 2 0 8 5 1395 5931 8000 2 0 8 5 1431 6082 8100 2 0 8 5 1467 6235 8200 2 0 8 5 1503 6390 8300 2 0 8 5 1540 6547 8400 2 0 8 5 1578 6705 8500 2 0 8 5 1616 6866 8600 2 0 8 5 1654 7028 8700 2 0 8 5 1692 7193 8800 2 0 8 5 1732 7359 8900 2 0 8 5 1771 7527 9000 2 0 8 5 1811 7697 9100 2 0 8 5 1852 7869 9200 2 0 8 5 1893 8043 9300 2 0 8 5 1934 8219 9400 2 0 8 5 1976 8397 9500 2 0 8 5 2018 8576 9600 2 0 8 5 2061 8758 9700 2 0 8 5 2104 8941 9800 2 0 8 5 2147 9127 9900 2 0 8 5 2192 9314 10000 2 0 8 5 2236 9503 10100 2 0 8 5 2281 9694 10200 2 0 8 5 2326 9887 10300 2 0 8 5 2372 10082 10400 2 0 8 5 2418 10278 10500 2 0 8 5 2465 10477 10600 2 0 8 5 2512 10678 10700 2 0 8 5 2560 10880 10800 2 0 8 5 2608 11084 Thermo Scientific Hematocrit Rotor 37 A RCF Values Speed rpm Rmin Rmax RCF Rmin RCF Rmax 10900 2 0 8 5 2657 11291 11000 2 0 8 5 2706 11499 11100 2 0 8 5 2755 11709 11200 2 0 8 5 2805 11921 11300 2 0 8 5 2855 12134 11400 2 0 8 5 2906 12350 11500 2 0 8 5 2957 12568 11600 2 0
22. 8 5 3009 12787 11700 2 0 8 5 3061 13009 11800 2 0 8 5 3113 13232 11900 2 0 8 5 3166 13457 12000 2 0 8 5 3220 13684 12100 2 0 8 5 3274 13913 12200 2 0 8 5 3328 14144 12300 2 0 8 5 3383 14377 12400 2 0 8 5 3438 14612 12500 2 0 8 5 3494 14848 12600 2 0 8 5 3550 15087 12700 2 0 8 5 3606 15327 12800 2 0 8 5 3663 15570 12900 2 0 8 5 3721 15814 13000 2 0 8 5 3779 16060 13100 2 0 8 5 3837 16308 13200 2 0 8 5 3896 16558 13300 2 0 8 5 3955 16810 38 Hematocrit Rotor Thermo Scientific Chemical Compatibility Chart c PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP B Chemical Compatibility Chart e EE z 2 Eas cc lt 5 BEL 6 de ce ce E Suu 588 E Xue me lt e 2 22 Tr tto Sune 2 Se tttrotsaweVe Oe eo ata 53 CHEMICAL zu aa a e 2 mercaptoethanol SSU SMS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Acetaldehyde S UU M U MUUUMM MS U S U Acetone Ms U SUM SUUSUSUUUSSUUSMMSUU Acetonitrile SSU 5 5 5 5 SU MU U 5 5 5 5 5 Alconox UUS 5 5 5 5 5 SS 9 6 5 5 5 5 5 5 5 5 5 5 SU Allyl Alcohol U 6 6 5 5 5 5 MS Aluminum Chloride UUS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 MU 5 5 Formic Acid 100 SMU U U 5
23. LEAR CLEARCRIMP CCCLEARCRIMP Nitric Acid 10 Nitric Acid 50 ANODIC COATING for ALUMINIUM a POLYURETHANE ROTOR PAINT c GLASS TYGON Nitric Acid 95 Hydrochloric Acid 10 Hydrochloric Acid 50 Sulfuric Acid 10 Sulfuric Acid 50 gt gt V c a NEOPRENE Sulfuric Acid conc co cl c c c c ww COMPOSITE Carbon Fiber Epoxy I I O c S Stearic Acid Tetrahydrofuran Toluene Trichloroacetic Acid gt NORYL V z c c STAINLESS STEEL Trichloroethane 2 2 c c z ALUMINUM z c ml v o DELRIN Trichloroethylene c c c c c o c c c c z a vv BUNAN c c Z S Z Z 9 WD oo NYLON Cile POLYCARBONATE cc a POLYVINYL CHLORIDE c c C C C Z C C
24. adapter can be autoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Clean the rotor before autoclaving and rinse it with distilled water Remove all accessories tubes adapters from the rotor Place the rotor on a flat surface Note No chemical additives are permitted in the steam CAUTION Never exceed the permitted temperature and duration when autoclaving If the rotor shows signs of corrosion or wear it must be replaced WARNING Dangerous materials through leaky seals The O ring of a sealed bucket will be damaged through heat exposure Remove it before autoclaving a sealed bucket Use a new O ring Thermo Fisher Scientific Service Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work Thermo Scientific Hematocrit Rotor 33 RCF Values RCF Values Speed rpm Rmin Rmax RCF Rmin RCF Rmax 300 2 0 8 5 2 9 400 2 0 8 5 4 15 500 2 0 8 5 6 24 600 2 0 8 5 8 34 700 2 0 8 5 11 47 800 2 0 8 5 14 61 900 2 0 8 5 18 77 1000 2 0 8 5
25. duce the stability of plastics Do not subject the centrifuge rotors and plastic accessories to direct sunlight Ifthe rotor shows signs of decoloration deformation wear or imbalance it must be replaced Thermo Scientific Hematocrit Rotor 25 6 Maintenance and Care Maintenance and Care Contents Cleaning Intervals on page 28 Cleaning on page 29 Disinfection on page 31 Decontamination on page 32 e Autoclaving on page 33 e Thermo Fisher Scientific Service on page 33 Thermo Scientific Hematocrit Rotor 27 6 Maintenance and Care Cleaning Intervals Cleaning Intervals For the sake of personal environmental and material protection you have to clean and if necessary disinfect the centrifuge on a regular basis Clean rotor chamber Daily or when polluted Clean rotor Daily or when polluted Accessories Daily or when polluted CAUTION Refrain from using any other cleaning or decontamination procedure than those recommended here if you are not entirely sure that the intended procedure is safe for the equipment Use only approved cleansers If in doubt contact Thermo Fisher Scientific Replace Sealing 1 Remove the old sealing 2 Form the new sealing into a ring without twisting it 3 Put the touching ends into the rotor groove so that they are placed between two capillary slots Touching Ends Press the cushion ring completely into the groove avoiding c
26. f aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Hematocrit Rotor 29 6 Maintenance and Care Cleaning Clean centrifuge and accessories as follows 1 Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adapters Use a neutral cleaning agent with a pH 6 8 for cleaning N A N N Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C 8 Clean the housing of the centrifuge as needed After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease part no 75003786 CAUTION When cleaning do not allow liquids especially organic solvents to get on the drive shaft the bearings the Auto Lock or the locks Organic solvents break down the grease in the motor bearing The drive shaft could freeze up After some
27. l damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe 8 Hematocrit Rotor Thermo Scientific 2 Rotor Specifications Rotor Specifications Contents e Thermo Scientific Heraeus 8 Centrifuge on page 10 e Thermo Scientific Sorvall ST 8 Centrifuge on page 11 Thermo Scientific SL 8 Centrifuge on page 13 Thermo Scientific Hematocrit Rotor 9 2 Rotor Specifications Thermo Scientific Heraeus Megafuge 8 Centrifuge Thermo Scientific Heraeus Megafuge 8 Centrifuge Table 1 Heraeus 230V 50Hz 60Hz Centrifuge Heraeus Megafuge 8 Catalog 75007211 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at nmax 16810 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK Table 2 Heraeus 120V 60Hz Centrifuge Heraeus Megafuge 8 Catalog 75007210 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at nmax 168
28. methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment For general radioactive decontamination use a solution of equal parts of 70 ethanol 10 SDS and water Disinfect the rotor and accessories as follows WN amp 10 32 Hematocrit Rotor Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adapters and dispose of them or disinfect them Rinse the rotor first with ethanol and then with de ionized water e Adhere strictly to the given application times Be sure the decontamination solution can drain off the rotor Rinse the rotor and accessories thoroughly with water Dispose of the decontamination solution according to the applicable guidelines Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease part no 75003786 Thermo Scientific 6 Maintenance and Care Autoclaving Autoclaving 1 Before autoclaving clean rotor and accessories 2 Place the rotor on a flat surface Rotors and
29. nning at top speed The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adapter exceeds the maximum permissible load e Reduce the fill level Reduce the speed Use the table or the formula permise adm Effective load admissible speed ngay maximum speed Actual Load g cavity RPM 0 2 13300 0 3 10859 0 4 9405 0 5 8412 0 6 7679 0 7 7109 0 8 6650 0 9 6270 1 0 5948 Using the Reading Graph The reading graph 76000938 is supplied with the rotor To read the values proceed as follows 1 Remove the hematocrit capillaries one by one from the rotor Note Be careful not to shake up the sample while removing If the samples have been shaken up during removal they can be centrifuge again 2 Place the bottom end of the hematocrit capillary on the zero line with the blood column and the upper end of the plasma column on the 100 line of the graph The dividing line between erythrocytes and plasma indicates the percentage of packed cells Note Be careful to place the hematocrit capillary at a right angle to the zero line 24 Hematocrit Rotor Thermo Scientific 5 Rotor Loading Rotor Life time Rotor Life time The rotor has no life time restrictions For safety reasons please bear the following in mind UV rays re
30. reases or waves 28 Hematocrit Rotor Thermo Scientific 6 Maintenance and Care How To Deal With Broken Hematocrit Capillary Tubes How To Deal With Broken Hematocrit Capillary Tubes Cleaning WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions Broken capillary tubes have sharp edges and are a risk of injury Remove the lid of the Hematocrit Rotor carefully Remove the larger pieces of the capillary tubes with tweezers Remove the rotor Remove the sealing slowly and carefully with the tweezers Clean and disinfect the rotor as described below Place a new sealing When cleaning the centrifuge Thermo Scientific Use warm water with a neutral solvent Never use caustic cleaning agents such as soap suds phosphoric acid bleaching solutions or scrubbing powder Rinse the cavities out thoroughly Use a soft brush without metal bristles to remove stubborn residue Afterwards rinse with distilled water Place the rotors on a plastic grate with their cavities pointing down If drying boxes are used the temperature must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface o
31. sted by HPA Porton down UK Thermo Scientific Hematocrit Rotor 11 2 Rotor Specifications Thermo Scientific Sorvall ST 8 Centrifuge Table 5 Sorvall 100V 50 60Hz Centrifuge Sorvall ST8 Catalog 75007202 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at Nmax 16810 min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK 12 Hematocrit Rotor Thermo Scientific Thermo Scientific SL 8 Centrifuge Table 6 Thermo Scientific 230V 50 60Hz 2 Rotor Specifications Thermo Scientific SL 8 Centrifuge Centrifuge SL8 Catalog 75007221 Weight empty kg 1 0 Maximum permissible load g 24 x 0 2 Maximum speed nmax rpm 13300 Maximum RCF value at N pax 16810 Max min radius cm 8 5 2 0 Pitch angle 90 Accel braking time s 14 24 Sample heating at nmax referred to ambient 16 temperature of 23 C running time 60 minutes Aerosol tight No Permissible temperature range autoclavable C 134 Tested by HPA Porton down UK Table 7 Thermo Scientific120V 60Hz Centrifuge SL8 Catalog 75007220 Weight empty kg 1 0 Maximum permissible load
32. supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or errorfree and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Original instruction manual 50139071 a printed in February 2013 WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermo com rohsweee for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country Contents iri a MN roe she he ne Corr eee eer a eee er rrr 7 items Supplied ian at 7 Intended USE 7 Precautions
33. tact aaa aa aa 47 6 Hematocrit Rotor Thermo Scientific Preface 1 Preface Before starting to use the rotor read through these instruction manual carefully and follow the instructions Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller s warranty Items Supplied Quantity Check Hematocrit Rotor 1 Anti corrosion Oil 1 Linear scale 1 Instruction manual 1 O If any parts are missing please contact the nearest Thermo Fisher Scientific representative Intended Use Thermo Scientific This rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by generation of Relative Centrifugal Force It separates human samples e g blood urine and other body fluids collected in appropriate containers either alone or after addition of reagents or other additives The rotor in the centrifuge is designed to run other containers filled with chemicals environmental samples and other non human body samples If the rotor is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Together with this hematocrit rotor the centrifuge becomes an in vitro diagnostics device to define the hematocrit value by centrifugation Apply DIN 58933 to define the hematocrit value To obtain reproducible results standardization is
34. tor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adapters and dispose of them or disinfect them Treat the rotor and accessories according to the instructions for the disinfectant spray or soak in solution Adhere strictly to the given application times Be sure the disinfectant can drain off the rotor Rinse the rotor and rotor lid thoroughly with water and then rub down Dispose of the disinfectant according to the applicable guidelines Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease part no 75003786 Hematocrit Rotor 31 6 Maintenance and Care Decontamination Decontamination Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all neccessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if needed CAUTION Before using any cleaning or decontamination
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