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3. Installation of the QuickPHOTO INDUSTRIAL 3.0

1. 7 t a w Image Settings part Gain the slider affects the signal s strength coming from the camera With higher gain values noise in the image may be more visible Brightness the slider sets camera s brightness Contrast the slider sets camera s contrast 208 Control Panels of Imaging Devices Gamma the slider is used to set gamma correction combination of the brightness and contrast adjustment Hue the slider enables to change image hue Saturation the slider is used for setting the color saturation Illumination Compensation Illumination Compensation the color balancing of the image le can be adjusted according to the used type of illumination Fluorescent Daylight Tungsten Xenon Halogen S Restore Default Settings clicking this button sets all values of the control elements in the Image Settings part to the default values White Balance White Balance sliders enable to manually adjust the level of each of the three color channels R red G green and B blue s Restore Default White Balance Settings clicking this button sets all values of the sliders in the White Balance part to the default values PE Black Balance sliders R G B R red G green and B blue enable to manually set the black balance In case the camera is set to grey scale mode or if you are using a monochromatic camera only one slider is available S Restore D
2. 4 Hardware Imaging Devices Analog Video Source Remote Trigger Magnification Encoders Illuminators fey Backup Settings a Load Settings Configuration Wizard OK Cancel Tab Remote Trigger This page is used for setting and verification of the remote trigger s functionality Remote trigger is an optional accessory which can be connected via serial or USB port If you want to use the remote trigger check the Use Remote Trigger option and select the COM port to which it is connected in case of the USB trigger we are talking about the USB Serial Port COM In the Remote Trigger Type part select the type of the used remote trigger Trigger with two buttons for example the two button foot switch is designed for image acquiring and for capturing of video recordings using the optional module for video recording Functionality of the remote trigger can be verified by pressing its button s If 185 Main Program Window the small square s change color to green after you press the button s and they turn back to black after you release the button s the remote trigger is set correctly and it is ready to be used Options x Srini vi Use Remote Trigger Default Folders ateata d H Image Names amp Saving Port Settings Sound Language Port COMi 1 Select a COM port to which is the remote trigger connected Do Not Show This Window Annotations f i 3 ae DR Appearance
3. Configuration Wizard ok Cancel 173 Main Program Window 7 2 Section Measurements Tab Units On this tab you may choose units which will be used for measuring micrometers or millimeters For the chosen units you may also choose the number of decimal places for displaying of the measured values Important The use of measuring accuracy in tenths of micrometers will only be relevant when the used microscope imaging device set provides such accuracy Way of expressing of the measured angle values can be set in the Display Measured Angle Values part Values can be displayed in Decimal form or In Degrees and Minutes Note Measured angle values will always be exported into Microsoft Excel program in decimal form irrespective of the settings of this option 174 Main Program Window Options x anes Units Display Measured Angle Values Default Folders Image Names amp Saving um Number of Decimal Places 0 Decimal Sound Language mm Number of Decimal Places Pa In Degrees and Minutes Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Backup settings g Load Settings Configuration Wizard
4. Zoom Magnification Changer Magnification Objective Position 1 4 10 2 20 a 40 4 Change Objective Positions Graph of Calibration Values 1 Calibration Value 1 mm _ _ _ C Objective Magnification OK Calibration of Individual Magnifications Using the Magnification Encoder for Upright and Inverted Microscopes When calibrating the individual magnifications proceed in a similar manner as described above for more information see Standard Calibration of Individual Magnifications on page 38 Major difference from the standard calibration of individual magnifications is the automatic filling of the Current Objective Magnifications field on 1 Magnification Selection page in the Calibrate New Magnification Calibration Calibration Name guide with values from the magnification encoder 50 Calibration of the Program for Measurements Calibrate New Magnification Calibration CCD Camera Upright Microsco X 1 Magnification Selection Define the magnification for calibration Current Objective Magnification Current Objective Position Zoom Magnification Changer Magnification Table of Magnifications after a correctly done calibration might for an upright microscope with the magnification encoder without the magnification changer look for example like this one Table of Ma
5. Black Balance Defined by Point option click the left mouse button on such a point of the image which will define the reference black color for the whole image Black balance will take place in the whole image based on the defined reference value This function is designed for fluorescence applications Adjust Brightness Contrast Gamma Adjust Colors Options Image Adjust Brightness Contrast Gamma and Image Adjust Colors or corresponding icons and open an Image Adjustment dialog box with tabs Brightness Contrast Gamma and Red Green Blue In the top part of both tabs there are sliders for individual options and in the bottom part there are previews of the image before any changes and after changes have been made Buttons and between both previews are there for the change of display size of both previews After previews have been zoomed 87 Main Program Window in it is possible to by dragging the mouse while holding the left mouse button move both previews Using sliders of individual functions it is possible to adjust the desired image parameters Changes will immediately be visible in the right hand side preview Default Values button in the bottom part of the window will bring all settings in this dialog box to the original state Changes made can be applied to the whole image by clicking the OK button Image Adjustment x Brightness Contrast Gamma amp Red Green Blue 20 Brightness
6. Key shortcuts used in imaging device control panels 298 List of Key Shortcuts Display in Full Screen Mode on the numeric keyboard Extension of the exposure time while using the manual exposure mode Page Up Extension of the exposure time while using the manual exposure mode on the numeric keyboard Shortening of the exposure time while using the manual exposure mode Page Down Shortening of the exposure time while using the manual exposure mode 299 List of Key Shortcuts 300 Troubleshooting FAQ QuickPHOTO INDUSTRIAL 3 0 Program How can I get the latest version of the program To get the latest version of the program go to the www promicra com downloads php website select the desired product and proceed according to the guidelines Which imaging devices are supported by the QuickPHOTO INDUSTRIAL 3 0 program List of supported imaging devices in the latest version of the QuickPHOTO INDUSTRIAL 3 0 program can be found on www promicra com cameras php website There appears an error message Unable to access rn xml or Unable to access config xml when terminating the QuickPHOTO INDUSTRIAL 3 0 program If you are using McAfee VirusScan virus protection program try to temporarily deactivate it If the problem disappears it will be necessary to create an exception in the virus protection program for the QuickPHOTO INDUSTRIAL 3 0 program OLYMPUS Digital Cameras E system CAMEDIA
7. ok Cancel 168 Main Program Window Tab Language This tab offers the option to choose a language of the QuickPHOTO INDUSTRIAL 3 0 program To change the language select the desired language from the list and click the Change Language button This operation may take a while Options x 4 General Default Folders Image Names amp Saving Language Notice some program parts may not be translated to all languages In such Sound case untranslated parts will display in English Language Cestina Do Not Show This Window English Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices Language Selection Analog Video Source Remote Trigger Magnification Encoders Illuminators Backup settings Gi Load Settings F Configuration Wizard ok Cancel Tab Do Not Show This Window Some dialog boxes in the QuickPHOTO INDUSTRIAL 3 0 program contain the Do Not Show This Window option If you check this option in some of the dialog boxes this dialog will not be displayed during the next activation of the corresponding function and the default action will be automatically done The corresponding option on this tab will be unchecked This setting will be remembered for the next run of the QuickPHOTO INDUSTRIAL 3 0 program If you want to restore displ
8. Build on Previous Numbering 00001 OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Tlluminators Default JPEG Quality Quality 95 High f Small File Large File fe Backup Settings a gt Load Settings f Configuration Wizard OK Cancel In the Store Images after Acquisition in part it 1s possible to select one of three options where the acquired images are supposed to be stored e Program Only new images will after acquiring be displayed in the Main Program Window Saving of images will be fully in the hands of the user e Program and Folder Automatic Saving if you activate this option acquired images will be displayed in the Main Program Window and at the same time they will automatically be saved into the folder selected in the Folder for Automatic Image Saving part For this function a folder selected as the default folder for image saving is set in the default state Active mode for automatic image saving will be indicated by the icon in the status bar in the right bottom corner of the program If you move the mouse over this icon information about the folder where the images are being saved will be displayed e Folder Only Automatic Saving if you activate this options newly acquired images will be automatically saved to a folder selected in the Folder for Automatic Image Saving part and they will not show in the Main Program Windo
9. Measurements on page 114 Measurements Annotations Imaging Devices Reports Window Hi Insert Horizontal Scale Bar Insert Vertical Scale Bar Line Segment Ellipse io1 0 amp Rectangle Circle t Polygon Polyline Count Objects gt ht 714 Angle gt Distance Between 2 Parallel Lines Arc Ne A Phase Analysis Hardness b Display Orthogonal Grid Display Circular Grid K H Export Measured Values to Microsoft Excel Continuously Units Set Active Calibration Calibration Manager Ctrl Alt C Menu Annotations Menu Annotations contains tools for indication of interesting areas in the image These are the tools available Text Line Arrow Rectangle Circle Ellipse Polygon and Freehand Menu Imaging Devices Menu Jmaging Devices is used for the choice of an active imaging device Only those devices whose support was chosen during program installation are on the list 69 Main Program Window _ Annotations Imaging i i Modules Reports Window Lumenera INFINITY Digital Camera Line uEye digital camera Arrow Rectangle Circle Ellipse Polygon Seneca ee one oe oe Freehand Menu Modules Menu Modules is used for activation of the modules e g built in Jime Lapse Video and Timer modules or other optional modules Reports Window Hel Time Lapse Video F5 Timer F6 Menu Reports Menu Reports contains functions for creatin
10. Time Lapse Video activates a built in Time Lapse Video module designed for creating of video files from the acquired time lapse images Timer activates a built in Timer module designed for an automatic acquiring of images in an adjustable time interval Best Fit Zoom displays the current image in such a size so it fits into the Display Window Zoom In increases the size of display of the current image by one step Zoom Out decreases the size of display of the current image by one step Zoom Actual Size 100 displays the current image in its real size 100 Display Size this field indicates current displayed image size It enables you to pick your desired size from the drop down menu of various values or you can using the keyboard type in a new value t f Main Program Window Crop after clicking on this icon you may select an area of the image which should be preserved Remaining part of the image will be removed Rotate Clockwise by 90 rotates the image to the right Rotate Counter Clockwise by 90 rotates the image to he left Flip flips the image around the horizontal axis Mirror mirrors the image around the vertical axis Resize opens a dialog box used for a change of the image size Adjust Brightness Contrast Gamma opens a dialog box for brightness contrast and gamma correction adjustment Adjust Colors opens a dialog box for color balance adjustment It is possible to indep
11. ok Cancel Tab Scale Bar This tab contains options for setting of the calibrated scale bar In the Scale Bar Type you may set the type of the scale bar Ruler Line Segment Staple and Stripe In the Scale Bar Size you may select one of the two automatic sizes Small Large or you may select the Custom option and type in your own desired length of the scale bar into the Custom Size field Color of the scale bar and its value can be chosen in the Scale Bar Options part by the Color of Scale Bar and its Value option Options Background Area and Background Color can change the area and color of the scale bar s background In the Preview part you can immediately see what will the scale bar look like when using the current settings 175 Main Program Window User defined settings of the scale bar can be saved into one of the four profiles To save the user defined settings to a profile proceed as follows 1 Make all the desired scale bar settings 2 Click the Save button in the Profiles part Save Profile dialog box will open 3 Select one of the four profiles you want to save the settings to from the Save Settings to Profile drop down menu 4 Enter the new profile name into the New Profile Name field 5 Save the settings by clicking the OK button If you check the Insert Scale Bar into Image Automatically after Magnification Assignment option in the Automatic Scale Bar Insertion part a scale bar will be inse
12. 2 On the Profiles tab click the Save to Profile button Save Profile dialog box will open 215 Control Panels of Imaging Devices Save Profile x 1 Select a profile you want to save current camera control panel settings to 2 Type a new profile name l Profile Name 3 In the Available Profiles part select by clicking the corresponding button one of the profiles you want to save the current control panel s settings to 4 Type in a new profile name into the Profile Name field 5 Confirm by clicking the OK button Loading a Profile Loading of a desired profile can be done by clicking the corresponding button of the profile on the Profiles tab Automatic adjustment of the control panel s elements will take place 216 Control Panels of Imaging Devices 2 Canon EOS Digital SLR Cameras Canon EOS 1100D Digital SLR Camera Se ee The control panel works with Canon EOS series digital SLR cameras Control panel of the Canon EOS digital SLR cameras is designed for control of camera functions displaying of the live view and image acquisition It consists of two tabs with control elements and the live view Image Tab This tab contains control elements for setting of main camera s parameters Settings will apply to the live view as well as to the acquired images 217 Control Panels of Imaging Devices i Image o gp Capture
13. 290 Modules B Time Lapse Video x Video Properties On this page select parameters of a new video file and click the Start button Video File Settings Interval between Frames Frame Rate Video Resolution 768x576 Video Duration 0 00 07 Timestamp V Date V Time Timestamp Settings lt Back Start Cancel In the Timestamp part you may set the insertion of a timestamp into a video file into each image of the video file will be inserted the information about the date and time of image acquisition This setting will be visible in the video file in such a way that the time will be running in it It is possible to insert Date and Time Appearance of the timestamp may be set after clicking the Timestamp Settings link Timestamp Settings When at least one of the Date and Time checkboxes is checked if you click the Timestamp Settings link a dialog box for setting of the timestamp will open Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which corner of the video file should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text 291 ar 292 Modules Timestamp Settings Preview 17 10 2012 13 52 45 To begin creating a video file click the Start button A dialog box for saving of the file w
14. 3 03 mm Line Segment Teneth 2 302 mm Line Seement Censh 3 303 mm Line Segment Ceneth 4 3 01 mm 5 3 Report Template Manager Report Template Manager Reports Template Manager dialog box is designed for managing of all of the already existing templates Selected template can be renamed or deleted 158 Main Program Window Report Template Manager Microsoft Word must not be running if you want to create a new template Template_1_Image Template_4 Images Rename Delete 6 Other Functions 6 1 Image Information Information about the current image can be displayed by activating the Image Image Information option by clicking the icon in the Toolbar or by clicking the Image Information 4 button in the controls of the corresponding thumbnail in the Thumbnail Browser Image Information Image Name dialog box which can have up to three tabs Image Metadata and Calibration will open Image Tab This tab gives the following information Path displays information about the image s location e Name corresponds with the image s name e Size shows the size of the saved image file e Width and Height show the image size in pixels 159 Main Program Window e Actual Width and Actual Height show the real size of the image in length units if the image has an assigned calibration e You may enter a text comment for
15. While creating a template Microsoft Word program must not be running Click the Create New Template button The New Report Template Wizard will open Continue in the following way 1 First page of the wizard informs in sum about the steps which will take place Click the Next button 2 On the next page of the wizard named Pattern Document Selection and Typing of a New Template Name click the Browse button and in the opened dialog box select the saved pattern document Type in the template name into the Type in a new report template name field Under this name the template will be available in the Template Manager Continue by clicking the Next button 152 Main Program Window New Report Template Wizard x Pattern Document Selection and Typing of a New Template Name Select an existing Microsoft Word pattern document which will be used as basis for creation of a new report template Type in new template name and dick the Next button 1 Select an existing Microsoft Word pattern document doc which will be used as basis for creation of a new report template Browse 2 Type in anew report template name max 32 characters lt Back Next gt Cancel 3 After clicking the Next button a pattern document will be loaded by the QuickPHOTO INDUSTRIAL 3 0 program On Processing Image of page all images found in the pattern document will be gradually displayed The wizard will give you a ch
16. Basler A601 fc JVC KY F1030 PixeLINK PL A662 Analog video source option for acquiring images from analog video cameras or other devices with analog video output video endoscopic systems ultrasonographs video recorders DVDs and so on TWAIN Device option for acquiring images from devices supporting TWAIN standard Using this interface it is possible to acquire images from a number of digital devices which are not natively supported by the program for example from digital video cameras scanners and many others 2 2 Opening of the Imaging Device s Control Panel To open control panel of the selected imaging device click the icon activate the Open Imaging Device Control Panel option from the File menu or use a F4 key shortcut 81 Main Program Window 2 3 Acquiring an Image In the open control panel of imaging device set the required parameters of exposition and acquire an image using the Capture button Acquired image will be immediately transferred to the computer and displayed in the Display Window For more detailed information about imaging devices control panels see Control Panels of Imaging Devices on page 189 3 Work with Images Description of work with images is divided into the following chapters Image Opening Image Editing Indication and Annotating of Interesting Areas Image Saving Image Printing ee a 3 1 Image Opening It is possible to open images using the File O
17. Digital Camera on page 14 4 Make sure the memory card is inserted in the camera only in case of OLYMPUS CAMEDIA and SP series consumer digital cameras 5 Make sure the memory card cover is closed 6 Make sure in the Device manager of the Microsoft Windows the camera device drivers have been properly installed See examples on the following pictures OLYMPUS E 3 E 30 E 620 E 520 E 450 E 420 E 510 E 410 Digital SLR Cameras 302 Troubleshooting FAQ E OLYMPUS Camera Controller BE OLYMPUS USB Camera e OLYMPUS E 330 Digital SLR Camera Set Disk drives w OLYMPUS E 330 USE Device e OLYMPUS CAMEDIA and SP Series Consumer Digital Cameras ge Disk drives See OLYMPUS crow USB Device If there is displayed an exclamation mark next to the device s name in the Device manager click the right mouse button on the name of the device and select the Uninstall option Turn the camera off and back on The drivers should automatically reinstall 7 Make sure the device for displaying of live view is correctly installed and set For more detailed description see the section called Installation of a Device for Displaying of Life View on page 11 Camera is not responding and it is not possible to turn it off How do I make the camera work again Before you turn the camera off it is necessary to always close its control panel first Only then it is possible to turn off and eventually d
18. Full Screen Ctrl F this button is used for displaying of live view full Full screen 275 Control Panels of Imaging Devices Image Settings Tab Image settings Adjust Crop borders Restart live image Brightness Hue Displayed area J J 720x576 lt a Contrast J Saturation J Adjust Brightness Contrast Hue and Saturation sliders can be used for setting the corresponding image parameters Click the Default button to restore the default values of all sliders Using controls in the Crop borders part there can be set the optimal size of the live view This way e g the unwanted black borders can be cropped from the image Displayed area Displayed area this indicator shows current size of the live view in pixels 720x576 Select click this button to display a dialog box for interactive selection of borders to be cropped from the live view Reset click this button to display the full size of the live view cropping of the borders will be deactivated Fz Restart live image click this button in case the live view display fails this may happen sometimes e g after returning from the screen saver mode Setting up the Borders to Crop After clicking the Select button there will appear a dialog box with static image from the live view in which you can select interactively the borders to be cropped Select the area of the image you want to retain by the green rectangle The rest will be cropped Borders can also be defi
19. Miscellaneous a 4 Measurements Units Scale Bar Sizes Colors Grid Stereomicroscopes Measurands Hardness Use Zoom Objective Encoder Other Settings 4 Hardware Imaging Devices Analog Video Source Remote Trigger Magnification Encoders Illuminators Ie Backup Settings a Load Settings J Configuration Wizard OK Cancel Adding a New Calibration with the Magnification Encoder for Upright and Inverted Microscopes Adding of a new calibration shall be done in a similar manner as described above for more information see Adding a New Standard Calibration on page 35 with the following differences 47 48 Calibration of the Program for Measurements In point 3 of the guide named Add Calibration Calibration Name on Magnification Encoder Selection page check the Objective Encoder for Upright Inverted Microscopes option and continue by clicking the Next button Add Calibration CCD Camera Upright Microscope x Magnification Encoder Selection Select magnification encoder you want to use for calibration CCD Camera Upright Microscope Only encoders configured in Edit gt Options gt Magnification Encoders menu are available Select Magnification Encoder No Magnification Encoder Manual Magnification Assignment Mode Objective Encoder for Upright Inverted Microscopes On page Assignments of Objective Magnifications to Nosepiece Positions set the corresponding ma
20. after one performed measurement automatically deactivated 118 Main Program Window Note Active measuring tool can always be terminated by clicking the right mouse button by repeated clicking on its icon or by the Esc key Measure Line Segment Tool Measure Line Segment can be activated by the Measurements Line Segment option or by clicking the lt icon Then by clicking into the image and by dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point a measuring line segment can be inserted Precisely horizontal or vertical line can be created if you hold the Shift key while inserting the line segment The length of the inserted line segment will be measured Measure Ellipse Tool Measure Ellipse can be activated by the Measurements Ellipse option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button insert the measuring ellipse You can rotate the ellipse after you have selected it by dragging the green grasp point displayed inside the ellipse You can measure a perimeter and area of an ellipse Measure Rectangle Tool Measure Rectangle can be activated by the Measurements Rectangle option or by O clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button insert the measuring rectangle You can measure a p
21. click this button in case the live view display fails this may happen sometimes e g after returning from the screen saver mode Setting up the Borders to Crop After clicking the Se ect button there will appear a dialog box with static image from the live view in which you can select interactively the borders to be cropped Select the area of the image you want to retain by the green rectangle The rest will be cropped Borders can also be defined manually by the fields at the bottom of the dialog box Confirm the settings by clicking the OK button The settings will apply to the live view only 248 Control Panels of Imaging Devices Crop borders Cropped borders Left 0 px Right a Top o g pa Selected area 720x576 2 cancei_ Shading Correction Tab This tab contains control elements for the shading correction function This function may be used to remove deficiencies in the image caused by inhomogeneous illumination removal of the darker corners of the image or by dust on the sensor Shading correction Shading correction Calibration mask 3648x2736 Correction level Disabled i 255 Enabled Er Illumination meee meee O Reflected Transmitted Shading correction Shading correction this option turns the Shading Correction function Disabled Enabled on off 249 Control Panels of Imaging Devices ee Illumination in dependence on the type of the used illumination selec
22. perimeter and area Measure Arc after you click this button function for measuring of an arc will be activated Then click the left mouse button gradually into three points in the live view through which the arc will be interspaced You can measure the arc s length measured on the perimeter diameter and the angle between the arc s arms Measure Ellipse Defined by 5 Points after you click this button function for measuring of an ellipse will be activated Then click the left mouse button gradually into five points in the live view through which the ellipse will be interspaced You can measure ellipse s perimeter and area Measure Arbitrary Angle after you click this button function for measuring of an angle will be activated Clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and the end point insert the first arm of an angle Then by clicking the left mouse button insert the second arm of an angle If you want to create a precisely horizontal or vertical arm hold the Shift key while creating it Measure Angle from the X Axis after you click this button function for measuring of an angle from the X axis will be activated By clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and the end point insert the first arm of an angle This arm will automatically be completed with a horizontal ax
23. you can lighten or darken the image using the Correction option nosens ISO sensitivity sets the sensitivity of the sensor The options conform to the ya sensitivity of traditional photo materials in the ISO scale 259 Control Panels of Imaging Devices ee i Exposure mode if the exposure mode is set to the Auto option A Manda 3 7 auto exposure time is automatically set according to the illumination of the O SFL Auto camera s field of view SFL Auto option represents automatic exposure mode for fluorescence imaging When using the Auto and SFL Auto modes the button Auto Exposure Lock will also be active This button locks the exposure time on the current value If the Manual mode is active value of the exposure time is set by user using the control elements in the Exposure time part Bacon Exposure region the controls in this part are designed for defining of ee an area of the live view which will be used for measuring of exposition O 1 g of expositio O01 in the Auto and SFL Auto modes You may select one of the predefined exposure region sizes 30 1 or 0 1 or you may select your own size of the region After selecting one of the options a frame will be displayed in the middle of the live view which will define the exposure region The frame can be moved to the desired area in the image move the mouse into the region and by dragging the mouse while holding the left mouse button relocate the
24. 3 Section Hardware Tab Imaging Devices Properties of the Imaging Device s Control Panels part contains the following options these options do not apply to imaging devices controlled via the TWAIN interface e Minimize Imaging Device Control Panel after Image Acquisition if this option is checked control panel of the imaging device will be minimized after acquiring of a new image 182 Main Program Window Display Imaging Device Control Panel on the Second Monitor for Dual Monitor Workstations if you check this option control panel of the imaging device will be displayed on the second monitor e Display Imaging Device Control Panel after Program Start up if this option is checked control panel of the imaging device will be automatically activated after launching of the QuickPHOTO INDUSTRIAL 3 0 program If you check the Close Twain Control Panel after Image Acquisition option in the Properties of TWAIN Imaging Devices Control Panels part control panel of the imaging device controlled via TWAIN interface will be automatically terminated after acquisition of an image If you leave this option unchecked TWAIN control panel will be active until terminated manually Options x CE Properties of the Imaging Devices Control Panels except for TWAIN control panels Default Folders Image Names amp Saving Minimize Imaging Device Control Panel after Image Acquisition png 1 Display Imaging Devi
25. 6 Hardness Test QuickPHOTO INDUSTRIAL 3 0 program is equipped with functions for evaluation of the hardness test of materials by Brinell method according to the standard EN ISO 6505 1 1999 and by Vickers method according to the standard EN ISO 4516 2002 Brinell Hardness Test Brinell hardness test uses to determine hardness indentation of a ball of a known diameter using a defined force into the material being tested A testing machine the so called hardness tester 1s used for this purpose Imprint can be scanned either directly by the hardness tester with a camera output or by a microscope imaging device set and it can be evaluated using the Brinell Hardness Test function Value of the material s hardness will be calculated after you enter the values of the operating load the size of the ball being indented and the duration time To evaluate Brinell hardness test proceed as follows 1 Acquire or open the image of the imprint in the Display Window and assign it a magnification which was used to acquire this image 2 Activate the function Brinell Hardness Test by the Measurements Hardness gt Brinell Hardness Test option or by clicking the icon The mouse cursor will change to a cross with a vertical line By clicking into the image place the line so it touches precisely the edge of the imprint Then place another line opposite to the first one Then in the same way place another two lines perpendicular to the first two
26. As dialog box has been opened all thumbnails of the images to be saved will be selected with an orange frame Thumbnails can be selected deselected in a similar way as the thumbnails in the Thumbnail Browser in the Main Program Window for more information see Thumbnail Browser on page 76 Settings done in the File Name and Type and Additional File Name Tags parts will always be applied to selected images from this list So you can set in bulk the name type and quality for more images at the same time Order of thumbnails on the list can be changed by dragging them with a mouse drag and drop Information about the number of images for saving and the number of selected images are displayed in the bar above the thumbnails If you move the mouse over one of the thumbnails several buttons will be displayed on the right side next to the particular thumbnail These buttons call for functions for a specific image Image preview in the full size can be displayed by clicking the Preview button 104 Main Program Window for JPEG image types the quality of the preview will be influenced by the set JPEG Quality parameter information about the image can be displayed using the Image Information button This function is useful for example if you want to add a description to the image before you save it Images can be removed from the saving list by clicking the Close button thumbnail will disappear from the list e i
27. Capture Tab The tab contains the controls for setting the camera parameters setting the exposure and acquiring images gy Capture E Quality Resolution Sensitivity White balance Capture 5Q vi 1280x960 he Auto ji Auto i E Program Shutter speed Anti Shock time Metering mode a Sequence Aperture Exposure compensation Center Spot v 1 3 0 v Capture after clicking this button an image will be acquired and Capture automatically transferred to the computer You can also use the Ctrl Enter E key shortcut Full screen the button is used for displaying the live view full screen In Fullscreen this mode you can control the application using the Capture and Exit full screen mode buttons in the upper part of the live view Connect the button is enabled only when the camera is not correctly Laen connected Click the button to re establish the communication Quality the image quality can be selected in this menu For some quality options the image resolution can be selected in the Resolution drop down menu Program in this drop down menu you can set the exposure mode The Manual option aperture and shutter speed can be set manually from Shutter speed and Aperture drop down menus The Aperture drop down A iori TA menu is enabled only when the lens is attached to the camera body The Auto option exposure parameters aperture and shutter speed are calculated automatically by the camera The shutter
28. Capturing geeesececscccsccccecceseoscsososeceesesssssssssoseessssessssssssesesessss R eceeecccccccccccceccccscscsososcocecossssososeecccescsssessssecosesessos Turn on Illumination before Capturing 5 gt E Turn off Illumination when Finish Illumination Control Turn off Current Illumination Intensity y e 2 h Intensity Set for Capturing a Y Time until Next Capturing 00 00 00 Number of Captured Images 0 Number of Remaining Images b Start 1 3 Recommendations for the Time Lapse Image Capturing To achieve optimal results during time lapse image capturing we recommend the following e Use manual exposure mode of the imaging device for image capturing Turn off the screen saver of the Microsoft Windows operating system Switch the automatic updates function to the manual installation mode e Use the computer s UPS e Before you start capturing images into the folder make sure that you are authorized to write into the folder and there is enough free space on the target drive 287 Modules e Because with most illuminators the illumination intensity decreases after turning on until temperature stabilization let the illuminator warm up and thermally equilibrate before you start the time lapse capturing 1 4 Time Lapse Image Capturing from the Imaging Devices Controlled via TWAIN Interface Timer module enables time lapse image captur
29. Color from Image tool cursor in the shape of a dropper the particular color shade of grey will be removed from the active phase Using a Threshold slider you can set the accuracy with which should the color shade of grey to be included in the phase correspond to the color shade of grey picked by the Measure Phase Pick Color from Image tool For finer setting of accuracy you can use a field with a numerical value next to the slider All phases will be after change of threshold recalculated and the change will then show in the image Part Preview Preview field displays enlarged part of an image around the cursor This enlarged preview helps to refine the choice of color shade of grey using the Measure Phase Pick Color from Image tool Part Overlapping of Phases In this part you can define whether individual phases may have common areas intersections or not If you choose the Disabled option then the area which was already calculated in one phase cannot be calculated in any other phase If you choose the Enable Use Color option any image area can be calculated in any number of phases Common areas will be indicated in the image with a color which is defined by a rectangle next to the Enable Use Color option After clicking the colored rectangle you can choose a different color for the common areas from the color palette 129 Main Program Window Part Calculate the Phase Proportion Relative to In this part you may ch
30. Controls part select the desired Threshold and possibly select a different phase color 7 Measuring of Other Phases If you want to measure other phases in the image select in the Active Phase Selection part another phase and repeat steps 5 and 6 8 Set Overlapping of Phases In the part Overlapping of Phases part select the desired mode of overlapping 9 Choose the Method of Calculation of Phase Amount In the Calculate the Phase Proportion Relative to part select the desired method of calculation of the phase amount Measured values will be displayed in the Phase Analysis dialog box in the Active Phase Selection part and also in the Table of Measured Values Active Phase Selection Phase 1 MM issx x Phase 2 MM sissy x Phase 3 MM 0 04 x Phase 4 MM 0 00 x Table of Measured Values Object Type Measurand Meas Nr Value Units Description Phase 1 Phase Share 1 84 Phase 2 Phase Share 5 51 83 Phase 3 Phase Share 6 0 04 Recommendation for Saving of Images with Measured Phases To preserve the measuring accuracy it is recommended to save images with measured phases with the layers kept as TIFF type Images of the JPEG type are being saved using a lossy compression and because of that a certain loss of information takes place As a result of this loss of information slight variations may appear in the phase analysis results when you open the image again 132 Main Program Window 4
31. Microsoft Excel program installed in your computer Clear All Measured Values this button clears the Table of Measured Cear Al Measured Values Jes and deletes all measuring objects from the live view i Delete Selected Value s this button deletes selected by mouse click Delete Selected value s value s from the Table of Measured Values Continuous Export of Measured Values to Microsoft Excel Measured values can be continuously exported to an open Microsoft Excel workbook To activate this function click the Export Measured Values Continuously to Microsoft Excel button on the Measurements tab After activation of this function measured values will be displayed not only in the Zable of Measured Values but they will also be immediately inserted into the open workbook of the Microsoft Excel program to the position of the cursor The cursor will then automatically move to the next cell Enter will be inserted Values will be exported without units and labels This function can be used for example for continuous automatic evaluation of measurements when the opened workbook in the Microsoft Excel contains suitable formulas Before you activate the function of continuous exporting the Microsoft Excel program must be running and a workbook with the permissions for writing must be opened in it To make the opened document accessible for continuous exporting the Microsoft Excel program must be running
32. Nr Value Units Description Measurand Length Navigator f a RA Parallel Lines Length 1 3 00 mm Number of Measurements 1 WOS Angle by 2 Lines Angle 2 101 46 Minimal Value 3 00 Maximal Value 3 00 SPN Average Value 3 000 Standard Deviation 0 000 4 Aili gt _ Sl amp gt amp lt al m amp Ta X B Export Values Hide Undock Dimensions Perimeters Areas Angles Objects Phases Hari Status Bar y 12227 60m x5 59mm INFINITY Digital Camera onl Table of Measured Values Size ratios of individual parts of the Main Program Window described above can be changed This change can be done in the following way shift your mouse over the border separating individual parts Mouse cursor will change to Click and hold the left mouse button and define the new size by moving the mouse Then release the button Note The default program layout can be restored using the Restore Default Program Layout option in the Window menu 63 Main Program Window 1 1 Menu Bar Menu File File menu contains the following functions Open opens images in the Main Program Window In the opened Open dialog box you may select one or more images and load them at once Option Open Recent offers up to ten lately used images for opening Options Save and Save All save images selected in the Thumbnail Browser respectively all opened images If the images being saved have already been saved before they will be saved again into
33. Orientation option enables to select the following options of orientation of the live view this option will also apply to acquired images Default sets the default orientation of the live view A Mirror mirrors the live view along the vertical axis a Flip flips the live view along the horizontal axis y Rotate by 180 rotates the live view by 180 degrees ain ica nates Cross displays hides the central cross manfo Grey Scale turns on off the grey scale mode Performance Test after clicking the aminini button several test images will be acquired to determine the minimum reachable time interval between images Several test images will be acquired during the test which can be deleted right after the test This 207 Control Panels of Imaging Devices test is automatically done during the first run of the control panel and normally there is no need to run this test again Colors Tab This tab contains control elements which are intended for adjustment of the parameters of the live view Changes in the settings will apply to the live view as well as to the acquired images Colors Image Settings S Gain y9 _ 9 Brightness v gt o Contrast e o DE Gamma O gt 1 Hue y 4 Saturation g gt 2 Illumination Compensation None White Balance S R G s e g
34. SFL Auto modes Exposure Time if a manual exposure mode is active you may using the arrow buttons or by dragging of the slider manually set the desired value of the exposure time To change the exposure time you may also use the and keys on the numerical keyboard or the Page Up and Page Down keys Value of the exposure time can also be set directly in minutes seconds milliseconds and microseconds using the corresponding editable fields under the slider This way is especially useful for setting of long exposure times When longer exposure times are manually set by the editable fields the live view may not correspond to the acquired images In such cases the value of the exposure time displayed above the slider will change its color to blue Exposure Region this control element is designed for defining of an area of the live view which will be used for measuring of exposition in the Auto and SFL Auto modes You may select one of the predefined exposure region sizes 100 the whole area of the live view 30 and 1 or you may select your own size of the region If you select the 30 or 1 option a frame will be displayed in the middle of the live view which will define the exposure region The frame can be moved to the desired area in the image move the mouse into the region and by dragging the mouse while holding the left mouse button relocate the frame to the desired 192 Control Panels of Imaging Devices p
35. Saturation the slider sets camera s saturation Saturation a Default use this button to restore all the settings on the Settings tab to the default values Shading Correction Tab This tab contains control elements for the shading correction function This function may be used to remove deficiencies in the image caused by inhomogeneous illumination removal of the darker corners of the image or by dust on the sensor 236 Control Panels of Imaging Devices Ea Sa k Shading correction Shading correction Calibration mask Correction level Disabled J m Enabled Light type Capture mask Reflected Transmitted Shading correction Shading correction this option turns the Shading correction function O Disabled on off Enabled Light type Light type in dependence on the type of the used illumination select eae F Reflected or Transmitted option The correction method is different for each illumination type Calibration mask Calibration mask if you click the Capture mask button an image which will be used as a calibration mask for shading correction function will be acquired In the field Capture mask f next to the button will be displayed the preview of the captured mask and information about its size Correction level Correction level using this slider you may set the y 255 strength with which will be the shading correction applied If you click the Preview in live image button you
36. Table of Measured Values 139 Main Program Window 61 Menu Bar 316 Index Menu Annotations 69 How to Use Phase Analysis Function Menu Edit 65 131 Menu File 64 Recommendation for Saving of Images Menu Help 70 with Measured Phases 132 Menu Image 66 Menu Imaging Devices 69 R Menu Measurements 68 Menu Modules 70 Menu Reports 70 Menu View 67 Menu Window 70 Modules 283 Deep Focus optional 293 S FLUO optional 294 System Requirements 313 RECORD IT optional 294 Time Lapse Video Built in Module T Reports 148 Creating a New Report 155 Creating New Report Template 149 Report Template Manager 158 288 or Troubleshooting FAQ 301 Timer Built in Module for Time Lapse ARTRAY ARTCAM Digital Cameras Image Capturing 283 309 IDS uEye Digital Cameras 310 O Live View from OLYMPUS Digital Options 164 Cameras 306 Section General 164 Live View from the Analog Video Section Hardware 182 Sources 309 Section Measurements 174 OLYMPUS Digital Cameras 301 Other Functions 159 OLYMPUS E 330 Digital SLR Compose New Image from Layout of Camera 305 Displayed Images 161 Other 311 Display in Full Screen Mode 164 QuickPHOTO INDUSTRIAL 3 0 Image Information 159 Program 301 Slideshow 163 W P Work with Images 82 Phase Analysis 125 Image Editing 83 Description of the Phase Analysis Dialog Image Opening 82 Box 126 Image Printing 108 317 Index Image Saving 97 Indication and A
37. and Layout mi o zz 2 Verify the remote trigger s functionality by pressing its button s The black i square s should turn green Miscellaneous 4 Measurements Units Remote Trigger Type as Single Button Trigger Two Button Trigger Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Bacup settings Gi Loadsettings Configuration Wizard ok Cancel Tab Magnification Encoders This tab is intended for setting of the magnification encoders To set the magnification encoder for upright and inverted microscopes check in the Upright Inverted Microscopes part the Use Objective Encoder option and from the Control Unit drop down menu select the desired control unit of the magnification encoder Correct functionality of the magnification encoder can be verified by clicking the Test Device button To set the magnification encoder for stereomicroscopes check in the Stereomicroscopes part the Use Zoom Objective Encoder option and from the Control Unit drop down menu select the control unit of the magnification encoder If the magnification encoder for objectives is also connected select it from the Objective Encoder Device drop down menu 186 Main Program Window Correct functionality of the magnification encoder can be verified by clicking the Test Device button Note Only t
38. ane aut sueead aes 164 72 SCHON Measure meN merine aea E E 174 Lo pechon El AL WARS cac isie kale SSAA a tnd E T Sad ashen E 182 Control Panels of Imasine D6Vvices c ciaendaniaieiiedutoiniees ea E a ats 189 1 Lumenera INFINITY Dre ital Cameras uratna aan E 189 2 Canon EOS Dibia SLR CAMEraS cokane iani a 217 3 IDS uEye Disita AMORA S cca hec sce cts snare E T ett eae 22 4 ARTRAY ART CAM Digital Cameras sso ceusswistitan dee eerih worn iadhacatusieieeks 233 5 PathScan Enabler IV Digital Microscope ccccccecec ee eeeeceaeeeeeeaeeeneenens 239 6 OLYMPUS E system Digital SLR Cameras i iicciasicses obneicelatiosabiabececeasaas 242 7 OLYMPUS DP72 Digital Camera ccccccceccscececteceeetsececerseeeeetseceseaes 255 8 OLYMPUS Consumer Digital Cameras SP and CAMEDIA series 268 9 Analog Video SOUL CE auceais taeda aa a a emcee eed 295 Modules taidscclhucuba salou nent Ment ncmhleentennan wal buciahant a a a 283 1 Timer Built in Module for Time Lapse Image Capturing 0006 283 lel Tao C apro eiie ad ea ee ean ie em Iran or sent ena eee ee as 283 K2 Pa MOm AMOI cur icantsh cet edontudtes aaa 286 1 3 Recommendations for the Time Lapse Image Capturing 287 1 4 Time Lapse Image Capturing from the Imaging Devices Controlled via OWAIN EGC TL ACS se Sere eth wciet e a e a alah satanic 288 2 Time Lapse Video Built in Module c ccc cece cece ee eeeee
39. are Brightness Contrast Saturation Hue Crop borders These settings do not influence the captured images Performed changes will apply to the live view only EW Live view Live view settings Crop borders Restart live view Brightness Contrast Saturation Hue Displayed area 720x576 gt gt gt gt Default 247 Control Panels of Imaging Devices Using the sliders you can set the properties of the live view The recommended procedure 1S 1 Open the control panel of the OLYMPUS E System digital camera capture the image and display it in the Display Window 2 In the control panel of the OLYMPUS E System digital camera activate the Live image tab Move the control panel to see the live view and the captured image at once 3 Using the sliders set the live view parameters so that it looks as similar as possible to the captured image By clicking the Default button you can restore the default settings of all the sliders in the Live image settings part Using the controls in the Crop borders part the unwanted borders of the live view can be cropped Displayed area Displayed area this indicator shows current size of the live view in pixels 720x576 Select click this button to display a dialog box for interactive selection of borders to be cropped from the live view Reset click this button to display the full size of the live view cropping of the borders will be deactivated Restart live view
40. can also use the Ctrl Enter key shortcut Full screen the button is used for displaying the live view full screen In Full screen this mode you can control the application using the Capture Refresh live image and Exit full screen buttons in the upper part of the live view Quality you can select from five quality levels Super High Quality High Quality and Standard Quality 1 and 2 and TIFF The SHQ and HQ modes capture images in full camera resolution in JPEG format with different compression ratios SQ1 and SQ2 modes capture images with lower camera resolution in JPEG format TIFF mode captures images in full camera resolution in TIFF format When using OLYMPUS SP 350 and SP 500UZ cameras the TIFF option is not available Sensitivity Sensitivity sets sensitivity of the sensor The options conform to the sensitivity of traditional photo materials in the ISO scale When Auto option is selected the camera changes the sensitivity automatically depending on the current illumination conditions White balance this option can be used for setting the white balance for various kinds of illumination Fluorescent 4 S500K Shade 269 Control Panels of Imaging Devices Flash mode Flash mode here you can set the flash mode If you are acquiring the images from a microscope turn the flash off Focus mode this drop down menu offers three options Auto Manual and Macro mode If you select the Manual mode you can
41. can be selected interactively If the window Text Annotation covers the inserted text annotation in the image we recommend to move this window to a different area by dragging it by its upper strip Inserted text annotation can be easily moved around in the image Click on the text annotation with the left mouse button and move the text by dragging the mouse while holding the left mouse button to the desired area 94 Main Program Window To edit an already inserted text annotation double click on it with the left mouse button A Text Annotation dialog box will open again and you will be able to edit the text as you wish Selection of the Inserted Objects for Editing If you want to edit objects that have already been inserted into the image it is necessary to select those objects first Proceed according to the following instructions Activate the Select tool and by clicking the contour line of the desired object select it Selected object will be highlighted with a green dash line and on its perimeter green grasp points will be activated except for an object created with the Draw Freehand tool Object selected this way is ready for editing To select more objects at the same time press and hold the Shift key and select all objects which you want to edit with the left mouse button To cancel the object selection click the left mouse button anywhere outside the objects or click the right mouse button Selection of ob
42. can be used for setting the white balance for various kinds of illumination 244 Control Panels of Imaging Devices Anti Shock time Anti Shock time delay between opening the mirror and the image acquisition can be set to avoid capturing of blurred images caused by vibrations Exposure compensation Exposure compensation this option allows to lighten or darken the images when the automatic exposure mode is set Warning When the battery indicator in the right upper corner of the tab changes its color to red replace the battery as soon as possible Low battery can cause unstable behavior of the camera Macro Tab This tab is visible only when a lens is attached to the camera body Macro Auxiliary light Flash mode ON Auto v lt Flash compensation Focus metering mode 21 3 v 11 Multi spot vi Auniliary light Auxiliary light auxiliary light helps the camera to focus correctly in dark illumination conditions 245 Control Panels of Imaging Devices peen camp neen Flash compensation it is possible to amplify or suppress the flash intensity by selecting the required value from this menu Flash mode here you can set the flash mode See the user s guide of the camera for more details Auto Fed Eye reduction Slow Red Eve reduction Slow synchronisation Focus metering mode here the area which will be used for evaluating the focus can be selected When using the E 330 Ce
43. compatible with documents in the Document Word docx format Pattern document for the template must be saved as the Document Word 97 2003 doc type 150 Main Program Window Decision about the purpose of individual images and tables contained in the pattern document will be done by the user in the second step Creating a New Template in the QuickPHOTO INDUSTRIAL 3 0 program QuickPHOTO INDUSTRIAL 3 0 program contains several beforehand predefined pattern documents They can be found in the QuickPHOTO INDUSTRIAL 3 0 Reports folder Individual sample pattern documents are prepared for 1 4 exported images including tables of measured values Above described instructions are clearly marked on the following picture of a sample pattern document example 1 image doc The logo image will remain unchanged Texts will remain unchanged This image serves as a mark where the images from the program should be placed This table serves as a mark where the table of measured values from the program should be placed 151 Main Program Window 2 Creating a New Template If you have created and saved the pattern document in the doc format activate the Report Template Manager Reports Template Manager function Report Template Manager dialog box will open Report Template Manager x Microsoft Word must not be running if you want to create a new template Important
44. d 2 OK Cancel 4 7 Editing of the Measuring Objects Selection of the Measuring Objects for Editing If you want to edit some of the already inserted measuring objects in the image it is necessary to make their selection first Proceed as follows Activate the Select tool and make the selection by clicking the contour line or the label with the measured value of the desired object Selected object will be highlighted with 136 Main Program Window a green dash line and on its perimeter green grasp points will be activated Object selected this way is ready for editing To select more objects at the same time press and hold the Shift key while making your selection and select all objects which you want to edit with the left mouse button To cancel the object selection click the left mouse button anywhere outside the objects or click the right mouse button Selection of the objects will be canceled Note Select tool is automatically activated every time no other tool is active Change of Line Color and Thickness of the Measuring Objects In order to edit inserted objects select them first for more information see Selection of the Measuring Objects for Editing on page 136 The color and line thickness of selected measuring objects may be changed For these purposes use the following options Edit Object Line Color Edit Object Line Thickness or the corresponding icon 3 in
45. displayed Thumbnail Browser images Only the visible part of each image displayed in the current layout will be printed e gt ore This window gives two options where to get images for printing Option Selected Thumbnail activates the print function for an image selected in the Thumbnail Browser Option Layout of Displayed Images is used for printing of the current image layout displayed in the Display Window This option prints on one page currently visible image parts displayed in the Display Window next to each other You may use this function for example if you want to print on one page various details of one image next to each other or if you want to for comparison print details of various images next to each other When using this function proceed as follows i Switch the Display Window to the mode for display of two or four images next to each other Display the desired images in individual Display Window fields This way you can also display one image in more than one field of the Display Window Main Program Window 3 Set the display size in individual fields according to your need and display the desired details 4 Activate the Print function in the Selecting the Source of Images for Print window choose the Layout of Displayed Images option and click the Next button After you click the Next button a Print window will open The resulting print layout may for two images dis
46. e IDS uEye UI 1440 C Digital Camera Run the uEye exe program from the Drivers gt IDS uEye gt UI 1440 C folder of the QuickPHOTO INDUSTRIAL 3 0 program installation CD e IDS uEye Other Supported Digital Cameras Run the wEye32 exe program for 32bit operating systems or uEye64 exe program for 64bit operating systems from the Drivers gt IDS uEye gt Others folder of the QuickPHOTO INDUSTRIAL 3 0 program installation CD Further on proceed according to the wizard s instructions Troubleshooting FAQ Important Install the device drivers from the QuickPHOTO INDUSTRIAL 3 0 program installation CD If you install newer device drivers delivered with the camera or downloaded from the internet the camera may not be working properly Live view is not visible or it is snatchy in the IDS uEye digital camera control panel 1 Make sure the performance of your computer corresponds with the minimal system requirements listed in the program s specifications 2 Make sure no computationally demanding program is running on your computer If such a program is running terminate it or wait until it is finished 3 In the control panel of IDS uEye digital cameras go to the Settings tab Click the Speed Adjustment button Readjustment of the IDS uEye digital camera s speed the number of displayed frames per second to correspond with your computer s performance will take place QuickPHOTO INDUSTRIAL 3 0 Program Other The d
47. elements which are intended for adjustment of the parameters of the live view Changes in the settings will apply to the live view as well as to the acquired images Image Settings Manual White Balance Edge Enhance Frame Rate Gain 9 gt 0 Black Level g 128 Red 9 gt 10 None FPS 19 5 Brightness g 100 Gamma g 1 00 Green g 10 O weak wa A 2s ae E r WI 8 Ones Gain the slider affects the signal s strength coming from the camera With higher gain values noise in the image may be more visible Brightness the slider sets camera s brightness Contrast the slider sets camera s contrast Black Level the slider controls the black color level Gamma the slider is used to set gamma correction combination of the brightness and contrast adjustment ae Mebane Manual White Balance sliders enable to manually adjust bed i i the level of each of the three color channels R red G green Green 10 and B blue Blue p 10 Edge Enhance Edge Enhance this option enables the enhancing of edges There are three None levels available None Weak and Strong enhancement O Weak O Strang Frame Rate Frame Rate displays how many images per second Frames per Second AGEN Bes FPS is the camera currently displaying in the live view 226 Control Panels of Imaging Devices Default use this button to restore all the settings on the Options tab to the default values Speed Adjustment starts the performance test to adjus
48. for each measurement e Single Measurement Mode if you activate this option the measuring tool will be after one performed measurement automatically deactivated Options x 4 General ae ais L Default Folders Mode of Assigning Magnifications to Images Image Names amp Saving Manual Magnification Assignment to Each Image Sound Language Automatically Assign the Last Used Magnification to a New Image Do Not Show This Window Annotations Settings for the Function Merge Objects with an Image Flatten Layers F pearance and Layout Here itis possible to set the size of the virtual window which will be used for flattening of layers This parameter Miscellaneous affects the font size of the measured values as well as the scale bar size in the flattened image Larger window size 4 Measurements will cause smaller font and scale bar size and vice versa Default option is 1024 x 768 Units Scale Bar Window Size 1024x768 X Sizes Colors Grid Measurement Mode Measurands Hardness Continuous Measurement Mode Selected tool remains active until deactivated Other Settings a as man Single Measurement Mode Single measuring object will be inserted upon tool activation Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators fe Backup Settings Ga Load Settings Configuration Wizard OK Cancel 7
49. frame to the desired position If you place the cursor above the frame s edge or above one of its corners the cursor will change shape and by dragging the mouse while holding the left mouse button you may change the size of the frame as you please By clicking the Center button you may place the frame to the center of the camera s field of view To define your own exposure region click the Custom button move the cursor to the live view and then by dragging the mouse while holding the left mouse button insert the frame of a desired size Live View Tab T Live view Live image quality Size 680 x 512 vi peter retired Sorerereterertenrrtsrtrt rr ererit Live image quality Live image quality from this drop down menu you may select the size size in which the live view will be displayed 260 Control Panels of Imaging Devices cose Cross displays hides the central cross C Display central cross Color Balance Tab This tab contains control elements which are intended for adjustment of the parameters of the live view Changes in the settings will apply to the live view as well as to the acquired images amp Color balance White balance a Black balance Bia 8s a oawoes Red o 60 one 25 D sue 0 EI White balance this part contains control elements for white balance function The Auto WB button activates the automatic white balance function After clicking the Region WB drag the mouse in the
50. i Full Screen 4272 x 2848 v Mode Auto Exposure Exposure Correction 0 7 Sensitivity ISO Power Supply AC Adapter Capture Ctrl Enter after clicking this button an image will be acquired and automatically transferred to the computer If the camera is not connected Full Screen Ctrl F this button is used for displaying of live view in full sm screen mode Using this type of display you can control the program using the Capture Ctrl Enter button which is placed in the left upper corner of the screen and the Exit Full Screen Mode button placed in the right upper part of the screen Image Size from this menu you may select the size in which the images will be acquired The available sizes will be affected by a potential use of the Crop Borders function from the Settings tab 218 Control Panels of Imaging Devices Exposure Exposure this part contains control elements for setting the camera s Mode Auto Exposure Exposure Correction Sensitivity ISO exposure The Mode indicator displays the current camera s mode set by its mode dial To acquire images from a microscope set the mode dial to Av automatic mode or M manual mode The 0 Auto White Balance Exposure Region Exposure Correction drop down menu enables you to lighten or darken the images when the automatic exposure mode is se
51. in the Microsoft Word format Created reports are in the form of a standard Microsoft Word document and you may edit them in Microsoft Word program as you please Important To use the function for creating reports it is necessary to have the Microsoft Word program installed on your computer in the XP or higher version QuickPHOTO INDUSTRIAL 3 0 program uses the predefined templates for creating of reports These templates are files in the Microsoft Word format and they contain information about the header structure the number of images and tables of measured values of the resultant report They may also contain texts or images for example a logo which will be contained in the resultant report In the QuickPHOTO INDUSTRIAL 3 0 program numerous different templates can be created and those can be used for creating 148 Main Program Window of reports according to your current need Creating a report template is a one act action Once you have created the template it will be saved in the program and you will be able to use it for creating of reports at any time 5 1 Creating New Report Template Necessary preparatory step for using of the function for creating reports is creating of the report template To create a new report template use the Template Manager Reports gt Template Manager function New template is created in the QuickPHOTO INDUSTRIAL 3 0 program based on the previously created Microsoft Word docum
52. line After the last line is placed they will be interspaced with a measuring tetragon This tetragon can be in case of need using the green grasp points modified so it precisely corresponds the imprint 133 3 Main Program Window Brinell Hardness Test dialog box will appear and the measured size of the imprint s diameter will be displayed in it Select the corresponding option according to the type of the used ball from the Test Type drop down menu From the Ball Diameter D menu select the corresponding size of the used ball and from the Mass m menu select the value of the used load in kilograms Enter the corresponding value to the Time t field In the Brinell Hardness field will be displayed the calculated material s hardness If the value has a red background it means the measured dimensions of the imprint do not comply the criteria defined by the standard EN ISO 6505 1 1999 and this measurement is not valid Note Ball Diameter D and Mass m menus contain only values corresponding with the standard EN ISO 6505 1 1999 If you want to enter different values write those values into the relevant fields using the keyboard Clicking the OK button will finish the measurement and the measured value will be displayed in the Table of Measured Values Double click on the inserted measuring tetragon will anytime bring about the Brinell Hardness Test dialog box for adjustment of the measuring parameters 134 Brinell Har
53. live view while holding the left mouse button to define a region which will be used as reference for the white balance The Red Green and Blue sliders enables to adjust the white balance manually Black balance this part contains control elements for black balance function The Auto BB button activates the automatic black balance function After clicking the Region BB drag the mouse in the live view while holding the left mouse button to define a region which will be used as reference for the black balance The Red Green and Blue sliders enables to adjust the black balance manually Shading Correction Tab This tab contains control elements for the shading correction function This function may be used to remove deficiencies in the image caused by inhomogeneous illumination removal of the darker corners of the image or by dust on the sensor 261 Control Panels of Imaging Devices i ee ka Shading correction Shading correction Calibration mask 4140x3096 Correction level Disabled Enabled Illumination 299 O Reflected Transmitted Shading correction Shading correction this option turns the Shading correction function Disabled on off Enabled pee Illumination in dependence on the type of the used illumination select Reflected Reflected or Transmitted option The correction method is different for Sra nma each illumination type Calibration mask 4140x3096 Calibration mask if you click
54. newer is required If you have an older version of firmware load a new firmware into your camera using the OLYMPUS MASTER program internet connection is necessary for update or contact an authorized OLYMPUS service How to Load a New Firmware 1 Make sure the camera s battery is fully charged and the USB cable is not connected 2 Inthe Settings 2 menu symbol of a key with the number 2 find the USB MODE item In this submenu select the STORAGE option Install the OLYMPUS MASTER program from a CD delivered with the camera Connect the camera to your PC using a standard USB cable delivered with the camera 5 If you are connecting the camera to your computer for the first time wait for installation of the camera device drivers contained in the operating system 6 Run the OLYMPUS MASTER program It is recommended to terminate all other programs ae 13 Installation 7 Click the Update Camera Language icon Click the OK button 8 Select the newest firmware and click the Update button Click the OK button 9 Wait until loading of the firmware to your camera is complete Switching the Camera to the PC Control Mode To control the OLYMPUS cameras from the QuickKPHOTO INDUSTRIAL 3 0 program it is necessary to switch them to the PC control mode Proceed as follows e OLYMPUS E system SLR Digital Cameras 14 Make sure the USB cable is disconnected from the camera Switch the mode dial to the P position and turn th
55. part Annotations Insertion Mode There are two options available e Continuous Inserting Mode if this option is active the selected tool will remain active for the current image until it is deactivated This way you may repeat inserting of the same type of object into the image without the need to activate the same tool over manually e Single Insertion Mode if you activate this option the selected tool will be automatically deactivated after one object has been inserted Setting the Line Color and Thickness To set the line color and thickness of the drawn objects use the Edit Object Line Color and Edit Object Line Thickness options or corresponding icon 7 in the Toolbar After the option Edit Object Line Color was activated or after clicking the left part of the l icon select the desired color from the displayed color palette After the option Edit Object Line Thickness was activated or after clicking the right part of the gt E icon select the desired line thickness from the displayed list Icon 77 also serves as an indicator of the currently set line color and thickness Selected line color and thickness will be used for inserting of all new objects Draw Line Tool Draw Line can be activated by the Annotations Line option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left 91 Main Program Window mouse button or by clicking th
56. part of the dialog box which is used for checking the correctness of the calibration procedure To continue click the Calibrate New Magnification button Calibration of the Program for Measurements Table of Magnifications Calibration CCD Camera Upright Microscope Table of Calibration Values mm Zoom Magnification Changer Magnification Calibrate New Magnification Objective Position 1 Graph of Calibration Values 1 Calibration Value 1 mm i Objective Magnification 2 Calibrate New Magnification Calibration Calibration Name guide will open on the first page Magnification Selection In the Magnification for Calibration part type in to the Current Objective Magnification field the value of objective magnification of the objective currently in use If you have chosen the No Zoom Magnification Changer option in step 4 while adding a calibration there will be an automatically given value 1 in the Zoom Magnification Changer Magnification part and you will not be able to change this value If you have chosen in step 4 one of the options Zooming Unit or Magnification Changer Unit the Zoom Magnification Changer Magnification field will be empty In this case fill in the value of the currently used zooming unit s magnification or the magnification changer s Continue by clicking the Next button 39 Calibration of the Program for Measurements C
57. phase amount will be used the whole image area without all defined regions e Overall Area of All Phases as a base 100 for calculation of the phase amount will be used an area indicated by all phases together Unmarked image areas will not be reflected in the calculation 130 Main Program Window Note If the Phase Analysis function is active it is not possible to work with other measuring objects in the image How to Use Phase Analysis Function To perform the Phase Analysis function proceed as follows l Select an Image for Phase Analysis Display the image in which you want to perform the phase analysis in the Display Window Activate the Phase Analysis Function Activate the Phase Analysis function by the Measurements Phase Analysis option or by clicking the 4 icon Select Measurement Area In the Measurement Area part select one of the three available modes Define Region s If you select the second or third mode in the Measurement Area part define the desired image region s for phase analysis respectively region s to be excluded from phase analysis Measure the Phase Using the Measure Phase Pick Color from Image tool cursor in the shape of a dropper pick the desired color shade of grey in the image For a more precise determination of the desired color shade of grey use the enlarged Preview Adjust the Threshold Set the Phase Color 131 Main Program Window In the Active Phase
58. scale will be inserted into the right bottom corner of the live view Insert Vertical Scale Bar after you click this button a vertical scale will be inserted into the right upper corner of the live view Display Orthogonal Grid after you click this button an Orthogonal Grid dialog box will be displayed In this window in the Spacing part enter the desired spacing of the grid for the X and Y axis Color and thickness of the grid lines can be set in the Line Options part Grid settings can be confirmed by clicking the OK button Display Circular Grid after you click this button a Circular Grid dialog box will be displayed In this window in the Spacing part enter the desired grid spacing Color and thickness of the grid lines can be set in the Line Options part Grid settings can be confirmed by clicking the OK button Measure Line Segment after you click this button the function for line segment measuring will be activated Then by clicking into the live view and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert the measuring line segment Precisely horizontal or vertical line segment can be created if you hold the Shift key when inserting it Length of the inserted line segment will be measured Measure Rectangle after you click this button function for rectangle measuring will be activated Then by clicking into the live view and dragging th
59. series cameras If you leave this option unchecked recommended the acquired image will be after transfer to a computer from the camera s memory card automatically deleted The Restore button in the Restore Default Device Settings part will restore the default settings of the device The Open button in the Advanced Settings opens the advanced settings of the device for manual setup Options x ee Live View from OLYMPUS E S SLR and Consu igital Ca Default Folders e View from S E System a mer Digi meras Image Names amp Saving In the first drop down box choose a device which will be used for live view display in the second one choose the video Sound standard Then select the input type Language Default video standard for European cameras is PAL B Do Not Show This Window pemen Input Annotations Appearance and Layout AV USB 2 0 Pro Miscellaneous Composite oe Video Standard mabe FE D S Vide B i re Scale Bar PALB Sizes Colors Restore Default Device Settings Advanced Settings Measurands 9 9 Hardness Restore Open Other Settings 4 Hardware ae Imaging Devices es ete Se OLYMPUS SLR Consumer Cameras Keep Images on the Memory Card Available for CAMEDIA and SP Series Only Analog Video Source Remote Trigger Magnification Encoders Illuminators fe Backup Settings a gt Load Settings Configuration Wizard OK C
60. settings into one of the profiles Default Profile if you click this button all elements of the control panel will be set to the default values Saved profiles will not be affected in any way by this action Saving of the Control Panel s Settings into the Profile To save the settings into the profile proceed as follows 1 Set the desired parameters of the control panel 2 On the Profiles tab click the Save to Profile button Save Profile dialog box will open Save Profile 1 Select a profile you want to save current camera control panel settings to Available Profiles E E Default 1 Default 2 a i Default 3 Default 4 2 Type a new profile name Profile Name 3 In the Available Profiles part select by clicking the corresponding button one of the profiles you want to save the current control panel s settings to 4 Type in a new profile name into the Profile Name field 5 Confirm by clicking the OK button 232 Control Panels of Imaging Devices Loading a Profile Loading of a desired profile can be done by clicking the corresponding button of the profile on the Profiles tab Automatic adjustment of the control panel s elements will take place 4 ARTRAY ARTCAM Digital Cameras Control panel of ARTRAY ARTCAM digital cameras enables to work with several types of digital cameras from the ARTRAY Inc Type of the connected camera is determined automatically at the startup of the control panel ARTCA
61. the Display Window use the method of dragging drag and drop or select a field in the Display Window at first in which would you like the image to be displayed and then click the left mouse button on the thumbnail of the desired image If you move the mouse over the thumbnail of one of the opened images several buttons will appear in its right upper part These buttons activate functions only for this particular image 76 Main Program Window Save Image button will save the image Print B button will print the image Image Information 4 button displays information about the image including meta data if those are available description and possible assigned calibration If measurements have been done in the image Export Measured Values button will also be active for export of the measured values into a file Mark as Important button will frame the thumbnail with a red frame for its highlighting Image can be closed using the Close button If some changes have been done to the image you will be asked whether you want to save these changes before you close this image New images that have not yet been saved and images that have been edited will have an asterisk displayed in front of their name for P00001 jpa example If measurements have been done in the image there will be an indicator Image contains unsaved measured values displayed by the thumbnail This indicator will disappear after the image is sa
62. the Shading correction function can be saved into one of the four profiles and at any time loaded again H Profiles Profiles be Save to profile E E 7 BS Default profile ProFile 1 Profile 2 Profile 3 ProFile 4 Save to profile if you click this button a dialog box will be displayed for saving of the current control panel s settings into one of the profiles Default profile if you click this button all elements of the control panel will be set to the default values Saved profiles will not be affected in any way by this action Saving of the Control Panel s Settings into the Profile To save the settings into the profile proceed as follows 1 Set the desired parameters of the control panel 2 On the Profiles tab click the Save to profile button Save Profile dialog box will open 266 Control Panels of Imaging Devices Save Profile 1 Select the profile you want to save current camera control panel settings to Available profiles Default 1 Default 2 Default 3 Default 4 2 Type a new profile name 3 In the Available profiles part select by clicking the corresponding button one of the profiles you want to save the current control panel s settings to 4 Type ina new profile name into the Profile name field 5 Confirm by clicking the OK button Loading a Profile Loading of a desired profile can be done by clicking the corresponding button of the profile on the Profiles tab Automatic ad
63. the Toolbar New line color of the selected objects can be chosen from the displayed color palette after the option Edit Object Line Color was activated or after clicking the left part of the E icon New line thickness of the selected objects can be chosen from the displayed list after the option Edit Object Line Thickness was activated or after clicking the right part of the TE icon Relocation Change of Size and Shape of the Measuring Objects In order to edit inserted objects select them first for more information see Selection of the Measuring Objects for Editing on page 136 If you want to move the selected measuring object in the image move the mouse cursor to 1ts contour line Cursor will change to S Clicking on the object s outline and by dragging the mouse while holding the left mouse button the object can be moved to the desired 137 Main Program Window area In the middle of selected objects of the ellipse rectangle and circle types there is a cross Using this cross you may also move these objects in the above mentioned way In the ellipse there is also a green grasp point connected by a dash line with its center Using this point you can rotate the ellipse any way you wish To change the size shape of the selected object proceed as follows move the mouse cursor to one of the green grasp points Cursor will change to u By clicking on the grasp point and by dragging the mouse while holdi
64. the calibration scale according to the number of marked segments and type the value in the Actual Length of the Line Segment field according to the measuring units chosen in the Units section To save the calibration value click the Finish button Value for new magnification will be added in the Zable of Calibration Values and Graph of Calibration Values 41 5 Calibration of the Program for Measurements Q Calibrate New Magnification Objective 10 Zoom 1 a x 3 Calibration Step 1 Drag a line segment to mark the maximum number of stage micrometer divisions Drag the line segment from the left border of the first division line to the left border of the final division line wo BD Step 2 Enter the actual length of the drawn calibration line and click the Finish button Length Units Actual Length of the Line Segment 600 um mm lt Back Finish Cancel Set another magnification for calibration on the microscope Change the objective in use or set a different value of magnification of the zooming unit magnification changer Complete calibration of the microscope imaging device set for all available microscope magnifications is done by repeating this procedure steps 1 to 5 The result of this procedure should be a situation when all the points in the Graph of Calibration Values in the Table of Magnifications Calibration Calibration Name dialog box will lay on a str
65. the four available profiles with the option to choose your own name If you activate the option Insert Scale Bar into Image Automatically after Magnification Assignment a scale bar will be automatically inserted into images after you select magnification in Objective and Zoom menus in the Toolbar which was used for their acquisition For more information about the scale bar settings see Tab Scale Bar on page 175 Further in the context menu there are four preset scale bar profiles These items correspond to the user definable profiles described in previous paragraph Activation of the selected scale bar profile can be done by selecting a profile name with a mouse Scale bar modifications will be reflected in all images 4 3 Standard Measuring Tools Measuring Mode Selection QuickPHOTO INDUSTRIAL 3 0 program offers two measuring modes Make your choice in Options of the program in section Measurements on the Other Settings tab Edit gt Options Measurements Other Settings in the Measurement Mode part Select one of the options e Continuous Measurement Mode if this option is active default option the selected measuring tool will remain active for the current image until turned off That way you can proceed with repeated measurements in the image without the necessity to manually activate the same measuring tool for each measurement e Single Measurement Mode if you activate this option the measuring tool will be
66. the image to the Description field If the description field is filled in for the image an indicator Image contains description will be displayed next to the image thumbnail in the Thumbnail Browser If the image is saved with the layers kept description will be saved as a part of the image file Metadata Tab Some imaging devices save into JPEG type images additional information about image acquisition the so called metadata for example sensor s sensitivity aperture number exposure time focal length information about the use of flash and so on This tab will display metadata of the image if they are available Calibration Tab This tab will only be displayed if the image has an assigned calibration On this tab information about the assigned calibration and the microscope magnification which was used for acquiring of the image are displayed 6 Change Image Calibration button opens a Change Image Calibration Image Name dialog box in which you can change calibration and magnification assigned to the image Calibration may also be removed from the image by clicking the Remove Calibration button Q Image Information samplel jpg x Q Image Information sample_9 jpg x Image Metadata Calibration Image Metadata Calibration SS e a Model E 510 Name sample 1 jpg Orientation Normal Size 547 kB ISO speed rating 100 X resolution 314 Width 2776 pixels Y resolution 314 Height 2074 pixels Reso
67. to measure in the image It is possible to measure up to 4 phases Colored rectangles indicate colors for marking of individual phases in the image To change the color click the colored rectangle and from the opened palette select a new color Numerical values next to the colored rectangles represent measured values of the percentage amount of individual phases These values are also displayed in the Table of Measured Values Buttons with crosses on the right hand side are there for removing of a specific phase from the image Part Active Phase Controls 128 Main Program Window This part contains controls for a phase selected in the Active Phase Selection part If the Display Phase button is pressed color coded a phase measured in the image will be indicated with the selected color If you want to for better clarity hide the phase indication undo the pressing of this button Indication of the phase will be hidden but the phase itself will remain included in the image This setting does not affect the measured values Invert Phase button will invert the phase If the Add Hue button is pressed color coded after marking of another color shade of grey in the image by the Measure Phase Pick Color from Image tool cursor in the shape of a dropper selected color shade of grey will be added to the active phase If you click the Remove Hue button after marking of another color shade of grey in the image by the Measure Phase Pick
68. 97 Number of Measurements 3 Line Segment Length 2 197 in _ Minimal Value 197 Line Segment Length 200 Maximal Value 200 Ellipse Perimeter Average Value Ellipse Perimeter Standard Deviation Ellipse Perimeter 4 x y BIG lt a 4A a AJ Export Values Hide Undock Dimensions Perimeters Angles Objects Phases Hardness Note If the tab with the image name contains measured values which have not yet been saved or exported symbol will be displayed in front of the image name Table of Measured Values Table of Measured Values contains values measured in the current image Individual rows are for better orientation displayed in a color which corresponds with the color of the measured value displayed in the image If the number of rows in the table exceeds the visible part of the window you can browse the table using the scrollbar on the right hand side the mouse wheel or the Page Up and Page Down keys If you select a row in the table using the mouse the corresponding measuring object in the image will be selected and vice versa if you select a measuring object in the image corresponding row will be highlighted in the Table of Measured Values You may enter a short comment for each measurement into a column named Description To enter your comment use a mouse to select the appropriate field type in your text into the Description column and confirm it by pressing the Enter key Proceed in the same way to e
69. AL 3 0 Program Program QuickPHOTO INDUSTRIAL 3 0 works with images of the JPEG TIFF PNG and BMP types The following paragraphs contain basic information about these file types e Information about JPEG File Type JPEG Quality The JPEG Joint Photographic Experts Group suffix jpg file type is the most commonly used type of file for saving photomicrographic images This is a file type utilizing the so called lossy compression Result of this 1s a relatively small data file size at the expense of a loss of image quality This loss of quality however means no problems for most photomicrographic applications Quality of the saved image can be affected by a parameter named JPEG quality This parameter has influence on the level of JPEG compression that way it affects the quantity of information details which 98 Main Program Window will be preserved in the image Higher JPEG quality means lower level of compression that way a larger saved file and vice versa JPEG type enables you to save besides the actual image data additional information metadata for example sensor sensitivity aperture number exposure time focal length information about the use of flash and other data thanks to the Exif extension Exif extension enables the QuickPHOTO INDUSTRIAL 3 0 program to use this file type for saving of images with the layers kept as they are Saving Parameters Set the JPEG quality for all selected JPEG images JPEG Quality Q
70. C key shortcut Pasting Image from the Clipboard Content of the clipboard if it contains image information can be pasted into the QuickPHOTO INDUSTRIAL 3 0 program as a new image This way you may paste an image from a different program or an image or its chosen area from the QuickPHOTO INDUSTRIAL 3 0 program When you paste an image from the QuickPHOTO INDUSTRIAL 3 0 program the new image will be pasted with merged layers To activate the paste function use the Edit Paste option click the icon or use the Ctrl V key shortcut Flipping and Rotating the Image It is possible to rotate the image by 90 clockwise or counter clockwise using the options Image Rotate Clockwise by 90 and Image Rotate Counter Clockwise by 90 or using C oe the 4 and icons 85 Main Program Window For vertical flipping or horizontal image mirroring use one of the options Image Flip or Image Mirror or one of the and icons Resizing Image To change the image size activate the Image Resize option or click the icon A Resize dialog box will open Type in one new image dimension second dimension will be calculated automatically in the original aspect ratio Ratio of the dimensions is always kept the same to maintain accuracy of measurements Confirm the new image size by clicking the OK button Width 2447 pixels Height 1919 pixels Width 300 pixels Height 627 pixels Aspect ratio will be m
71. C100 XP card is not compatible with Microsoft Windows 7 8 operating systems Turn off your computer and unplug it Insert the card into an empty PCI slot as far as possible from all other cards and screw it in Plug in the computer and turn it on Ifa Found New Hardware wizard opens up terminate it Installation of the Leadtek WinFast VC100 XP device drivers will take place during the installation of the QuickPHOTO INDUSTRIAL 3 0 program Installation Installation of OLYMPUS Digital Camera When installing the OLYMPUS digital camera proceed according to the following steps e Inspection update of the camera s firmware only OLYMPUS E 330 SLR e Switching the camera to a PC control mode e Connecting the camera with the computer e Installation of the digital camera device drivers Individual steps are described in the following chapters Inspection Update of the Camera s Firmware only OLYMPUS E 330 SLR camera Note Other types of cameras do not need firmware updates To control the OLYMPUS E 330 SLR camera from the QuickPHOTO INDUSTRIAL 3 0 program it is necessary to have the firmware version 1 2 or newer installed in the camera Current version of firmware can be detected the following way turn on the camera unplug the USB cable press the MENU button and go to Settings 2 menu symbol of a key with the number 2 here select the Firmware item the very last item Firmware Body 1 2 or
72. Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Objective Position 4 m 17993 Remove Selected Magnification Remove All Magnifications Change Objective Positions E E ou v el gt c o 5 o a UO _ _ _ Objective Magnification 43 Table of Magnification after a correctly done calibration might for a stereomicroscope Calibration of the Program for Measurements with one objective and a zooming unit look for example like this one Table of Magnifications after a correctly done calibration might for a stereomicroscope Table of Magnifications Calibration CCD camera stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Objective Position 0 7 0 8 1 125 1 6 2 25 32 1 47 4308 14 9644 12 0085 9 5681 7 4295 5 9150 4 7123 3 6450 RemoveSeleciedMagnifcalion Remove All Magnifications nge Objective 4 Hil gt Graph of Calibration Values T 3 ke gt 5 5 2 a 9 Zoom Magnification OK with two objectives and a zooming unit look for example like this one 44 Calibration of the Program for Measurements Table of Magnifications Calibration CCD ca
73. Control Panels of Imaging Devices Orientation Orientation this field serves as an indicator of the set orientation of the timestamp If you double click into this field a Timestamp settings dialog ADC box will open _ Preview in this field preview of the timestamp is 12 10 2012 17 07 22 displayed the way it will be inserted into the acquired images Position the indicator shows the position where the timestamp will be inserted in the acquired images Timestamp Settings If you click the Settings button or 1f you double click into the Orientation field a dialog box for setting of the timestamp will open Timestamp settings Format Font type Arial Font color E Black C Bold Timestamp size 30 of image width Background Color O White C Transparent Date Format dd mmiyyyy Position Orientation top left top right 2 bottom left bottom right Preview 12 10 2012 17 07 22 Cancel Format using the options in this part it is possible to set the appearance of the timestamp 265 Control Panels of Imaging Devices Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text Profiles Tab All settings of the control panel except for the settings of
74. Installation of the Dongle on page 17 Installation of the program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 Installation completion for more information see Installation Completion on page 18 Individual steps are described in the following chapters Installation 1 Installation of Imaging Devices 1 1 Installation of Digital Cameras and Other Imaging 10 Devices Installation of the Lumenera INFINITY Digital Cameras For now do not connect the Lumenera INFINITY camera to the computer If it is connected disconnect it Continue with steps nstallation of the QuickPHOTO INDUSTRIAL 3 0 Program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 and Installation Completion for more information see Installation Completion on page 18 Installation of the Canon EOS Digital SLR Cameras Connect the Canon EOS digital SLR camera to your running computer turn it on and switch the mode dial to the Av automatic mode or M manual mode position Wait until the camera s device drivers will be automatically installed Continue with step Installation of the QuickPHOTO INDUSTRIAL 3 0 Program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 Installation of the IDS uEye Digital Cameras Important IDS uEye UI 1440 camera is not compati
75. M 300MI camera i Image Settings Shading lt Image quality Profiles sows WB 10244768 v m pg screen Exposure mode Exposure time Cross O duto AEL i D 1 155 C Display central cross Manual The control elements are located in three tabs Image Tab This tab contains control elements for setting of the main camera s parameters Settings will apply to the live view as well as to the acquired images 233 Control Panels of Imaging Devices Image Image quality Profiles Capture Full screen i Exposure mode Exposure time Cross So g 1 155 Display central cross n Capture Ctrl Enter after clicking this button an image will be acquired E and automatically transferred to the computer Full screen this button is used for displaying of live view full screen Using Fullscreen this type of display you can control the program using the Capture Ctrl Enter button and the Exit full screen mode button placed in the upper part of the screen se Connect this button is enabled only when the camera is not correctly Connect connected Click the button to attempt to re establish the communication Auto WB this button activates the function for automatic white balance It stale is best to do this balancing when the camera is capturing a white area site eV Image quality here select the resolution in which the images should be acquired Bolo Profiles the control panel
76. O INDUSTRIAL 3 0 program Any images may be used as substitute images Important is only their size and location Their graphic content is not relevant because these images will not be a part of the resultant report they only serve as marks for placement of images from the QuickPHOTO INDUSTRIAL 3 0 program Tables inserted to the sample document may have two different purposes These may be e tables which shall be preserved in the resultant report for example tables defining the format and layout of the document e substitute tables which will in the template represent the so called marks which means they will define the location of tables with measured values which will be exported to the report from the QuickPHOTO INDUSTRIAL 3 0 program Table format number of columns rows formatting can be of one s own choice because the resulting format of the exported tables will be adjusted to the measured values from the QuickPHOTO INDUSTRIAL 3 0 program Note To achieve proper functionality of report creation it is necessary to the tables and images in the pattern document set the text wrapping style to square or in line with text Pattern document may also contain any amount of texts These texts will be in an unchanged form preserved in the resultant report Save the pattern document as Document Word 97 2003 doc type and close the Microsoft Word program Important QuickPHOTO INDUSTRIAL 3 0 program is not
77. Off v Mode Disabled v Count Capture Full screen z i Exposure time ISO sens Exposure mode Exposure region Time 1 150s Correction o v Manual 30 200 v o omn Se O SFL Auto 00 1 E Capture captures the image and transfers it to the computer You can also use Capture the Ctrl Enter key shortcut 258 Control Panels of Imaging Devices E Full screen the button is used for displaying the live view full screen In this Full screen mode you can control the program using the Capture and Exit full screen mode buttons in the upper part of the screen Image Size here select the resolution of acquired images Size 4140 x 3096 w Binning Binning this option enables the binning function You can select from Off 2x2 and 4x4 modes roaa cues Accumulation after you turn on the accumulation mode More Cout t and click the Capture button the camera will capture defined number of images From these images one image will be created and transferred to the computer You can select from the Integral or Average modes Integral mode creates the resulting image by summing the partial images Average mode creates the resulting image by averaging the partial images Exposure time Exposure time if a manual exposure mode is Time 1 150 s Correction active you may using the arrow buttons or by J dragging of the slider manually set the desired value of the exposure time When Auto or SFL Auto mode is active
78. Pack 3 Make sure you are signed in to an account with administrator s privileges Make sure the camera is connected to the computer using the USB cable Make sure you have selected the correct device for live view displaying in the QuickPHOTO INDUSTRIAL 3 0 program s Options Make sure you have selected in the QuickPHOTO INDUSTRIAL 3 0 program in Imaging Devices menu the OLYMPUS E system SLR camera option Make sure the battery in the camera is fully charged Make sure the firmware version 1 2 or newer is loaded in the camera Current version of the firmware can be found out the following way turn on the camera disconnect the USB cable press the MENU button go to the Settings 2 symbol of a key with the number 2 and here select the item Firmware the last item Firmware Body 1 2 or newer is necessary If your firmware version is lower load a new firmware into your camera using the OLYMPUS MASTER 2 program internet connection 1s necessary for the update or you can contact the authorized OLYMPUS service Run the Camera Setup wizard again Program Files Common Files OLYMPUS service setupcam exe and proceed according to the wizard s instructions 10 Turn the camera off and back on 305 Troubleshooting FAQ Live view from OLYMPUS Digital Cameras E System CAMEDIA and SP series Important Neither the Leadtek WinFast VC100 XP device nor the Grand AV USB 2 0 device white USB device delivered with the previous
79. Positions 45 Calibration of the Program for Measurements 3 2 Creating a New Calibration Using the Magnification Encoder for Upright and Inverted Microscopes To make measurements in the QuickPHOTO INDUSTRIAL 3 0 program more comfortable it is possible to for upright and inverted microscopes use the so called magnification encoder It is an optional equipment connectible to the computer which automatically determines current position of the encoded nosepiece with objectives When acquiring an image corresponding magnification is automatically assigned to the newly acquired image Therefore it 1s not necessary to assign the microscope s magnification used for obtaining of every image manually before measuring Before creating a new calibration put the stage micrometer under the microscope set the lowest magnification and focus the microscope The process of creating a new calibration using the magnification encoder consists of three main steps e setting of the magnification encoder e adding a new calibration with the magnification encoder for upright and inverted microscopes calibration of individual magnifications using the magnification encoder for upright and inverted microscopes Setting the Magnification Encoder 1 Connect the magnification encoder to the control unit connect it to the computer and install its drivers according to the manual supplied with this device 2 Run the QuickPHOTO INDUSTRIAL 3 0 progra
80. QuickPHOTO INDUSTRIAL 3 0 User Guide QuickPHOTO INDUSTRIAL 3 0 QuickPHOTO INDUSTRIAL 3 0 Copyright 2012 PROMICRA s r o All rights reserved Table of Contents Produ CON oi daca uaioceansatiatete tana pun etal ineal aa ioe A E EE 7 EE ETE EA n Sehgal A EET EA E AE AE T EE A S A E T A E A A bune 9 l T stallation of Imagine Gy COS onenian sensn tras ar A E EAN EE 10 1 1 Installation of Digital Cameras and Other Imaging Devices 10 1 2 Installation of OLYMPUS Digital Cameras E system CAMEDIA and gel eA ict ne ete eee CRN eee ete Te eg Re TL Oe RE Meena ee Ne wn Ree ee ee 11 2 mS TaAOUOF he WON lE miert es oE EA EEEE 17 3 Installation of the QuickPHOTO INDUSTRIAL 3 0 Program 64 17 A stalan Ome GOmp ICU Om tasaa eeta a a a 18 4 1 Completing the Installation when Using the Lumenera INFINITY Digital C E E A E ARE 18 4 2 Completing the Installation when Using the IDS uEye Digital AIMS eat chat santae a din niauamaueus EE 19 4 3 Completing the Installation when Using the PathScan Enabler IV Digital MICO CODE penne Ren ae SRO Ra eee ere NT te ero eet ane en ee nC GE 20 4 4 Completing the Installation when Using the ARTRAY ARTCAM Digital lt P AMM ChAS aha chat Matas Gono abatrsaneiedt aa a aianaeateubueral mame 21 4 5 Completing the Installation when Using the OLYMPUS E 330 SLR Digital E a E E es iat alall aa mena acne dee needa 21 4 6 Completing the Installation when Using
81. Switch the camera to the P mode using the mode dial turn it on and open the memory card cover Card cover open warning will appear Then first press the OK menu 1 button and along with it the Quick View button with older types turn on off the LCD screen 2 Hold both buttons for about 3 seconds Menu CAMERA SDK SETUP will appear In the USB part select the CONTROL option with older types the option On confirm by pressing the OK button and close the memory card cover Olympus 8080WZ 15 Installation Camera should be now ready for computer control The camera will not be accessible as a Mass Storage disk drive after switching to the PC control mode If you want to download images from the camera like from a disc unit the camera must be switched back to the STORAGE mode with older types OFF Connecting the OLYMPUS Camera with the Computer Note AV USB 2 0 PRO device is equipped with the following connectors e Yellow CINCH for connection of life view output from the camera e Red CINCH for connection of the right channel of the audio signal source e White CINCH for connection of the left channel of the audio signal source Black S Video for connection of S Video analog video signal source To connect the OLYMPUS camera use the yellow CINCH connector 1 To control OLYMPUS E system and SP series cameras from the computer it is necessary to use a special dual cable USB live view CINCH connector of this c
82. Unit PRO M CB1 30W Test Device OLYMPUS SLR Consumer Cameras ipse Ga Load Settings Configuration Wizard OK Cancel Control Panels of Imaging Devices QuickPHOTO INDUSTRIAL 3 0 program is able to capture images from several types of imaging devices These devices can be controlled via dedicated control panels 1 Lumenera INFINITY Digital Cameras J Lumenera INFINITY 1 5C Digital Camera ox B Live view BG Profiles i 6 mmen i EJ Bi vA 6 EA E m g Capture i FullScreen Auto WB Region WB Region Table of Measured Values E merens E Image amp Colors Image Size 2592 x 1944 z Exposure Mode Exp Correct Auto AEL Manual 0 SFL Auto Region BB Default BB a Table of Measured Values Control panel of Lumenera INFINITY digital cameras enables to work with several types of digital cameras from the Lumenera Corp Type of the connected camera is determined automatically at the startup of the control panel If more than one Lumenera INFINITY camera 1s connected to the computer a dialog box will be displayed at the startup of the control panel for selection of a camera which is supposed to be connected Control panel of the Lumenera INFINITY cameras is designed for control of camera functions displaying of the live view and image acquisition It consi
83. Video Source Remote Trigger Magnification Encoders Illuminators Bacup Settings Gi LoadSettings F Configuration Wizard ok Cancel Tab Other Settings Mode of Assigning Magnifications to Images part offers two options e Manual Magnification Assignment to Each Image if this option is active default option it is necessary to manually set magnification from the Objective or eventually Zoom menu to each image intended for measurements e Automatically Assign the Last used Magnification to a New Image if you activate this option to each new image will be automatically assigned the last magnification assigned to some image In the Settings for the Function Merge Objects with an Image Flatten Layers part it is possible to set the size of the virtual window which will be used for flattening of layers Setting of the Window Size value has affect on the font size of the measured values and scale bar size in the flattened image Larger window size will cause smaller font and scale bar size and vice versa Default option is 1024 x 768 pixels Measurement Mode part offers two options 181 Main Program Window e Continuous Measurement Mode if this option is active default option the selected measuring tool will remain active for the current image until manually deactivated That way you can proceed with repeated measurements in the image without the necessity to manually activate the same measuring tools
84. __ gt Ressnsnsesesenosenssssenessossessssnssosssoesonsssensesssessensssensessssnssonsosssessesnossnsssssnesscssesses 240 Settings the Image Resolution DPI drop down menu enables to set the required scanning resolution of images The Brightness Contrast and Gamma sliders enable to adjust the corresponding parameters of the preview and scanned images The performed settings will apply to the preview during next preview acquisition These settings will also apply to the scanned images The S button resets all the control panel s parameters to the default values Acquire Preview after clicking this button a preview of the whole Slide will be acquired and displayed in the Preview part Scan Image after clicking this button an image of the area defined by a rectangle displayed in the Preview part according to parameters set in the Settings part will be scanned and transferred to the computer Control Panels of Imaging Devices es ponnnanssnsneasncenssansensscossennssanseassossnssnsenssnnnsessosnssansecsscsssonssesssnssscessonssensanendl Connect this button is displayed instead of the Scan Image button in case the device is not properly connected Click this button to connect the device Preview in this part the preview of the slide is displayed after clicking the Acquire Preview button respecting the settings of Brightness Contrast and Gamma sliders in the Settings par
85. a f sample2 jpg 1194x 973 FEG Quality 95 Two indicators may be displayed in the thumbnail area according to the current situation If there is an already existing image with the same name and type in the folder chosen in the Location part an indicator will be displayed by the thumbnail Unless you change the name or type of the image the already existing image in the folder will be overwritten If the indicator is displayed by some of the thumbnails it means that there has been a collision of the names as well as the types of the images on the list In such a case saving of the images cannot take place unless you change the name or type of these images Legend for the displayed indicators will be displayed in the nformation part 1 amp eo i x sample 1 jpa x 4804 JPEG Quality 95 Location Part In this part it is possible to select a folder into which all images displayed in the Thumbnails of Images for Saving part will actually be saved Control in this part is similar to the standard Windows dialog box for saving files 105 Main Program Window Location J Samples m oje je BE W a samplel jpg Desktop 57 sample4t png Part File Name and Type In this part it is possible to choose the names and types of images selected in the Thumbnails of Images for Saving part If you have selected more than one thumbnail and you type in a new name to the File Name
86. able should be connected with the CINCH connector of the device for life view display Connect the USB connector of the dual cable to the USB port of the computer In case of need use extension cables 2 Make sure the camera s mode dial is set to the P position and the camera is on Installation of the Digital Camera Device Drivers e OLYMPUS E system SLR Digital Cameras Device drivers of the OLYMPUS E system SLR cameras will be installed during program installation e OLYMPUS CAMEDIA and SP Series Digital Cameras 16 Installation Device drivers of the OLYMPUS CAMEDIA and SP series cameras will be installed automatically after connecting of the camera to the computer and turning it on 2 Installation of the Dongle Connect the dongle included in the delivery to the USB port of your computer If a Found New Hardware wizard appears terminate it Dongle device drivers will be installed during program installation 3 Installation of the QuickPHOTO INDUSTRIAL 3 0 Program Note Installation of the QuickPHOTO INDUSTRIAL 3 0 program must be done from the administrator s account signed in user must have the administrator s privileges If you do not have the administrator s privileges contact your administrator Important Do not install into the operating system any codec packs e g All In 1 K Lite Mega Codec Pack and so on These packages may cause incorrect function of the program If you do have some of
87. able to set custom image print size in length units If the Constrain Proportions option is checked and you will type in the first dimension the second dimension will be calculated automatically in the original aspect ratio of the image This option is available only for print of one image on a page Scale options in this part enable to set custom image print size in percents compared to the original size If the Constrain Proportions option is checked and you will type in the first dimension the second dimension will be automatically calculated in the original aspect ratio of the image This option is available only for print of one image on a page In the Image Alignment part you can set the alignment of the images on the page by clicking one of the 9 buttons Part Image Information sets print of additional information about the image Along with the image it is possible to print after checking the corresponding options Image Name Date and Time of Image Origin and Description for more information see Image Information on page 159 Buttons in the Position part determine the position towards the image where additional information will be located Information 1s for better orientation highlighted with a green color in the Preview This highlight will not show in the final print In the Image Borders part select whether the printed images should have a black frame When printing more images on one page it is possible to set the Im
88. age Spacing in the chosen length units 113 Main Program Window Buttons in the Bottom Part Default Settings button returns all settings in the Print window to the original values Print button will begin print on the selected printer it will close the Print window and it will also store the set parameters for further opening of the Print window Cancel button will close the Print window without storing the set parameters Apply button will store the set parameters for further opening of the Print window actual print will not take place Note Print parameters are always stored only within the current QuickPHOTO INDUSTRIAL 3 0 program run 4 Measurements QuickPHOTO INDUSTRIAL 3 0 program is equipped with many functions for measurements in the image These functions can be found in the Measurements menu Corresponding icons are located in the Toolbar There are the following functions available inserting a calibrated scale bar 4 measuring of the line segment length measuring of the perimeter and area of an ellipse measuring of the perimeter and area of a rectangle measuring of the diameter perimeter and area of a circle amp measuring of the perimeter and area of a polygon measuring of the polyline s length 2 counting of objects measuring of arbitrary angle fa angle from the X axis angle from the Y axis K angle defined by two line segments measuring of the distance between two parallel li
89. aging device 2 Open the control panel of the imaging device 3 Perform the image acquisition 2 1 Selection of the Imaging Device In the Imaging Devices menu choose the imaging device which you want to use for image acquisition In this menu you will find those imaging devices whose support was selected during the program installation If you want to add another device to this list proceed according to the following chapter see Adding the Support of a New Imaging Device on page 22 Below is given the list of all imaging devices being currently supported e LUMENERA Infinity Digital Camera option for LUMENERA Infinity digital cameras Type of the camera in use will be detected automatically e Canon EOS Digital SLR Camera option for Canon EOS series digital SLR cameras 80 Main Program Window uEye digital camera option for IDS uEye digital cameras Type of the camera in use will be detected automatically OLYMPUS Digital Camera option for OLYMPUS CAMEDIA and SP series cameras Type of the camera in use will be detected automatically OLYMPUS E system SLR camera option for OLYMPUS E system SLR cameras Type of the camera in use will be detected automatically ARTRAY ARTCAM camera option for ARTRAY ARTCAM digital cameras Type of the camera in use will be detected automatically OLYMPUS DP72 camera OLYMPUS DP20 camera OLYMPUS DP70 DP71 DP30BW PathScan Enabler IV Digital Microscope
90. aight line respectively on straight lines If some of the graph points significantly stand out from the line it means that there has been a mistake in the calibration process In this case choose the deviating magnification by clicking the relevant value in Table of Calibration Values or by clicking the deviating point in the Graph of Calibration Values and click the Recalibrate Selected Magnification button Then in a similar manner as in steps 2 to 4 recalibrate the chosen magnification to the correct value Table of Magnifications after a correctly done calibration might for an upright microscope without the magnification changer look for example like this one 42 Calibration of the Program for Measurements Table of Magnifications Calibration CCD camera upright microscope x Table of Calibration Values fmm Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Remove Selected Magnification Remove All Magnifications Change Objective Positions Graph of Calibration Values 1 Calibration Value 1 mm Objective Magnification Table of Magnifications after a correctly done calibration might for an upright microscope with the magnification changer look for example like this one Table of Magnifications Calibration CCD camera upright mic with mag changer x Table of Calibration Values mm
91. aintained to preserve measurement accuracy Automatic White Balance After you have activated the Image Auto White Balance option automatic balancing of the white color will take place in the whole image White Balance Defined by Point After you have activated the Image White Balance Defined by Point option click the left mouse button to such a point in the image which will define the reference white color for the whole image White balance based on the chosen reference value will take place in the whole image 86 Main Program Window Sharpen After you have activated the Image Sharpen option the whole image will be sharpened Blur After you have activated the Image Blur option the whole image will be blurred Median Filter After you have activated the Jnage Median Filter option median filter will be applied to the whole image This function will remove some point defects such as the so called hot pixels or some types of image noise from the image This function is suitable especially for fluorescence applications Negative After you have activated the Image Negative option the image will be transferred to a negative If you repeat this function image will be brought to its original state Grey Scale After you have activated the Image Grey Scale option the image will be transferred to a grey scale image Black Balance Defined by Point After you have activated the Image
92. alibrate New Magnification Calibration CCD Camera Upright Microsco X 1 Magnification Selection Define magnification for calibration Magnification for Calibration Current Objective Magnification 10 Zoom Magnification Changer Magnification 1 Next gt Cancel Calibration of the new magnification upright microscope without the magnification changer Calibrate New Magnification Calibration CCD Camera Stereomicroscope X 1 Magnification Selection Define magnification for calibration Magnification for Calibration Current Objective Magnification Zoom Magnification Changer Magnification Next gt Cancel Calibration of the new magnification stereomicroscope with a zooming unit 3 Next page of the guide 2 Image Source Selection offers a choice whether to acquire the calibration image from the active imaging device or whether to use the already existing image opened in Display Window In the Image Source part choose Acquire a New Image from the Imaging Device Continue by clicking the Next button 40 Calibration of the Program for Measurements Calibrate New Magnification Objective 10 Zoom 1 x 2 Image Source Selection Select the source of images for calibration In case of acquisition of a new image from a microscope place a stage micrometer under the microscope Image Source eeeeeecesssscsoeseessecosossesseeessssocsosececeesesecse
93. alues in the current image After you click the Measurands button in the left bottom part of this tab Measurands dialog box will open In this dialog box you can using the checkboxes select which measurands should be measured by individual measuring tools and their text labels Measurands samplel jpg x Length Diameter Radius Perimeter Area Angle Distances Lengths 7 Cirde v Rectangle Polygon lt lt 5 A S S S S Ellipse 143 Main Program Window In the Font Size of Scale Bar Value part it is possible to select the font size of the scale bar value and in the Dimension and Extension Lines and Leaders part thickness and color of these lines Performed changes are interactively being displayed in the current image Change Image Calibration button opens a Change Image Calibration dialog box in which you may change the calibration and the magnification assigned to the current image Calibration can also be removed from the image by clicking the Remove Calibration button Change Image Calibration sample4 jpg x Assign the Following Calibration to the Current Image Calibration Name CCD Camera Upright Microscope Objective 20 z Zoom 4 9 Exporting Measured Values Exporting Values from the Table of Measured Values Measured values with eventual descriptions can be exported into a file in the xls format for further elaboration in the Microsoft Excel program or into the form of a
94. amera device drivers will be installed This process must be repeated one more time for installation of the second driver 4 5 Completing the Installation when Using the OLYMPUS E 330 SLR Digital Camera If you have restarted your computer sign in again to the administrator s account from which the program has been installed Program Camera Setup will run automatically Continue according to the wizard s guidelines If the Camera Setup will not run automatically run it manually using the icon on the desktop or run it from the system drive from the folder Program Files Common Files OLYMPUS service setupcam exe 21 Installation 2 Continue according to the wizard s guidelines You will be asked to turn the camera off and back on 4 6 Completing the Installation when Using the OLYMPUS CAMEDIA and SP Series Digital Cameras Note OLYMPUS CAMEDIA and SP series cameras cannot be used from the Guest account If you want to use the program from the administrator s account all you have to do is turn on the camera and then run the QuickPHOTO INDUSTRIAL 3 0 program If you want to use the program from common user accounts it is necessary to obey the following procedure after program installation we are speaking only about the very first run of the program 1 Turn off the camera and sign off from the administrator s account 2 Sign in to your user account and turn on the camera Wait until communication between the came
95. ample 1 jpg sample2 jpg sample4 jpg sample3 jpg Measurement Settings sample 1 jpg Measurand Length Number of Measurements 1 Minimal Value 3 00 Maximal Value 3 00 Average Value 3 000 Standard Deviation 0 000 1194 x 973 8 46mm x 6 89mm Lumenera INFINITY Digital Camera pme Warning QuickPHOTO INDUSTRIAL 3 0 program is not designed for diagnosis and determining of medical procedures Introduction Installation Important It is necessary to have the administrator s privileges for installation If you do not have administrator s privileges contact your administrator When installing the QuickPHOTO INDUSTRIAL 3 0 program proceed according to the following steps L Installation of imaging devices for more information see Installation of Imaging Devices on page 10 Installation of digital cameras and other imaging device for more information see Installation of Digital Cameras and Other Imaging Devices on page 10 Installation of OLYMPUS cameras E system CAMEDIA and SP series e Installation of a device for displaying of live view for more information see the section called Installation of a Device for Displaying of Life View on page 11 Installation of OLYMPUS digital camera for more information see the section called Installation of OLYMPUS Digital Camera on page 13 Installation of the dongle for more information see
96. ance to decide with each image what shall be the purpose of the currently displayed image in the template If the image is supposed to be a fixed part of the report for example a logo check the first option No this image is a fixed part of the report e g logo and should be retained If the displayed image serves as a mark for inserting of images from the QuickPHOTO INDUSTRIAL 3 0 program check the option Yes this image is a mark where the image from the program should be inserted when creating a new report If you check the second option it is possible to change the image size in the template To preserve the original layout of the pattern document it is recommended to keep the original image sizes Continue by clicking the Next button 153 4 154 Main Program Window New Report Template Wizard x Processing Image 2 of 2 Define properties of the displayed image and dick the Next button Is the image below a mark which shows where to put the image from the QuickPHOTO INDUSTRIAL 3 0 program in the final report No this image is a fixed part of the report e g logo and should be retained Yes this image is a mark where the image from the program should be inserted when creating a new report What should be the size of the inserted image in the final report Same image size as in the pattern document Image Size in Pattern Document 181mm x 136mm Custom Size Width 181 mm Height 136 g
97. ancel Tab Analog Video Source This tab will be available only if the support of analog video sources has been installed and it is intended to set the digitalization device for acquiring images from these sources If you are using a digitalization device delivered with the QuickPHOTO INDUSTRIAL 184 Main Program Window 3 0 program it will be configured automatically after you see this tab When using a different digitalization device select the device from the Device drop down menu and select the norm of the video signal from the Video Standard menu In the Input part select the desired type of the used connector The Restore button in the Restore Default Device Settings part will restore the default settings of the device The Open button in the Advanced Settings opens the advanced settings of the device for manual setup Options x 4 General Default Folders Analog Vileo Sources Image Names amp Saving In the first drop down box choose a device which will be used for analog sources in the second one choose the video Sound standard Then select the input type Language Do Not Show This Window meea Input Annotations Appearance and Layout AV USB 2 0 Pro Miscellaneous Composite 7 a Video Standard Units F Scale Bar PALB S Video Sizes Colors 2 Restore Default Device Settings Advanced Settings Measurands Hardness Restore Open Other Settings
98. and SP series How to switch the OLYMPUS digital camera to the mode for PC control Proceed according to the instructions in the section called Installation of OLYMPUS Digital Camera on page 14 The camera cannot be connected in the QuickPHOTO INDUSTRIAL 3 0 program 1 Inthe QuickPHOTO INDUSTRIAL 3 0 program in the Imaging Devices menu make sure that you have selected the corresponding control panel for the used camera type 301 Troubleshooting FAQ for OLYMPUS E 3 E 30 E 620 E 520 E 450 E 420 E 510 E 410 digital SLR cameras select the OLYMPUS E system SLR camera v 2 option for OLYMPUS E 600 digital SLR camera select the OLYMPUS E system SLR camera option Note after opening the control panel the text OLYMPUS E system SLR camera v 2 will be displayed in its title for OLYMPUS E 330 digital SLR camera select the OLYMPUS E system SLR camera option for OLYMPUS CAMEDIA and SP series consumer digital cameras select the OLYMPUS digital camera option If you do not see the desired item on the list run the installation of the QuickPHOTO INDUSTRIAL 3 0 program and install the support for your type of camera 2 Make sure the camera is turned on and connected to your computer by the USB cable If you are using a battery make sure it is fully charged 3 Make sure the camera is switched to the PC control mode for more information see the section called Installation of OLYMPUS
99. angle Then in a similar way insert a line segment defining the second arm of an angle The arms of an angle will be created from both of the line segments by elongation using auxiliary lines Convex angle will be measured If you want to measure an angle of more than 180 drag with a mouse the label with the measured value to the second half plane Measure Distance Between 2 Parallel Lines Activate a tool for measurement of two parallel lines by the Measurements Distance Between 2 Parallel Lines option or by clicking the icon By clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert the first line Then by clicking the left mouse button to the desired point insert the second parallel line Distance between both lines will be measured Precisely horizontal or vertical line can be created if you while inserting it hold the Shift key Measure Arc Activate a tool for arc measurement by the Measurements Arc option or by clicking the icon Using the left mouse button gradually insert three points which will define the arc You can measure the length measured along its perimeter diameter and an angle created by the arms of an arc 122 Main Program Window 4 4 Displaying a Grid of Defined Dimensions You can choose from two types of grids of defined dimensions which can be inserted into the current image displayed i
100. anted Region s of the Image from Phase Analysis M after you have activated this option it is possible to define one or more regions in the image which will be excluded from the whole image area determined for phase analysis This option is useful for example for elimination of darker corners or local defects Part Tools This part contains several buttons Measure Phase Pick Color from Image Z after you have activated this tool move the mouse cursor cursor in the shape of a dropper into the image and click on the desired color shade of grey whose percentage amount in the image would you like to measure You may include more than one color shade of grey into each phase by consecutive clicking This tool is available for all modes from the Measurement Area part Select Region after you have activated this tool you can select one or more when holding the Shift key regions defined in the image The regions will be framed in green color after you have selected them If you select the polygonal region there will be green grasp points displayed in its vertexes Using these grasp points you may change the shape size of the region Selected region can be erased at any time This tool is active only for the second and third mode from the Measurement Area part Define Region Polygonal Area gt after you have activated this tool define by clicking the left mouse button vertexes of the polygonal region in the image Region will be close
101. ask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in and set the desired exposure time 7 In the Correction level part click the Preview in live view button According to the need you may using the Correction level slider adjust the correction Reflected light 1 Set the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Place a white sheet under the microscope for example a sheet of white paper Do 238 not focus the microscope and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view Control Panels of Imaging Devices is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black Click the Auto WB button automatic white balance on the mage tab On the mage tab set the desired image quality 5 Switch to the Shading correction tab and select the Enabled option In the Light type part select the Reflected option Then click the Capture mask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in focus the microscope and set the desired exposure time 7 In the Correction level part click the Preview in live view button According to the need you may using the Correction level slider adjust the correction E 5 PathScan E
102. asure a perimeter and area of a polygon Note If the edges of the inserted polygon will cross each other in a certain point value of the area will equal 0 Only the polygon s perimeter will be calculated Measure Polyline Tool Measure Polyline can be activated by the Measurements Polyline option or by clicking on an arrow on the right next to the icon and by selecting the Polyline option Then by clicking the left mouse button on a chosen point in the image insert the beginning point of a polyline Repeated clicking on the left mouse button will define the break points Precisely horizontal or vertical line can be created by holding the Shift key while creating it To finish inserting of the polyline click the right mouse button There will be measured the length of the polyline 120 Main Program Window Count Objects This function can be activated by the Measurements Count Objects Count Objects New Group option or by clicking the icon Click the left mouse button on every object which you want to include in the counting Each counted object will be indicated with a cross and the number of counted objects will be displayed in the Table of Measured Values Counting can be terminated by clicking the right mouse button repeated clicking the icon or by pressing the Esc key All objects of the same group will be indicated with a cross of the same color The same color will be used for the corresponding r
103. asure convergent or obtuse angle check the Convergent Obtuse Angle option This option will be applied to the last measured angle and all other newly measured angles a Delete All Measuring Objects from Live View this button will erase all measuring objects from the live view Values in the Table of Measured Values will not be affected by this function e Undo Last Measurement Ctrl Z this button will undo the last done measurement from the live view and at the same time it will remove the corresponding measured value from the Table of Measured Values 199 Control Panels of Imaging Devices Export Measured Values Continuously to Microsoft Excel this button activates function of continuous exporting of measured values to the Microsoft Excel program Settings this button opens a dialog box for settings of measurements in the live view Table of Measured Values Values measured in the live view are continuously being displayed in the Table of Measured Values in the bottom part of the control panel Measuring objects in the live view and measured values displayed in the Table of Measured Values behave independently meaning that the measuring objects can be deleted from the live view without having any effect on the values in the Table of Measured Values and vice versa It is possible to write a short description to each measurement in the Table of Measured Values E Display Hide Table of M
104. ated by the Annotations Polygon option or by clicking the icon Then by clicking the left mouse button into the selected area in the image insert the first polygon s vertex Repeated mouse clicks of the left mouse button will insert other vertexes of the polygon Precisely horizontal or vertical edges can be drawn if you hold 92 Main Program Window the Shift key To finish drawing of the polygon click the right mouse button the last vertex will be connected with the first one and that way the polygon will be closed Draw Freehand Tool Draw Freehand is activated by the Annotations Freehand option or by clicking the gt icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw the desired curve Insert Text Annotation Tool Insert Text Annotation is activated by the Annotations Text option or by clicking the 4 icon Cursor will change into a cross with the letter A Then click the left mouse button into the image to the spot where the text annotation should be placed A Text Annotation dialog box will open Text Annotation x Background Color Orientation Transparent Custom Color Abc Background Area J Rectangle Abc Wa lt r qy Behind Text Only B Z UJ 35 Aral 2 Sample text Type the text content into the bottom field Setting of the font properties can be done using the Bold B I
105. ation mark already exist in the current folder and will be over Information ave Additional File Name Tags Part Files for Export This part contains a list of all files intended for export All files intended for export will be indicated with a frame after you have opened the Export Measured Values dialog box Files can be selected deselected in a similar way as thumbnails in the Thumbnail Browser 145 Main Program Window in the Main Program Window for more information see Thumbnail Browser on page 76 Settings done in the File Name and Type and Additional File Name Tags parts will always be applied on the files selected in this list That way you may set the name and type for more files at the same time Besides the file names according to the current situation two indicators may be displayed If some of the files already exist in the folder selected in the Location part there will be an indicator displayed next to the file names If you do not rename the files or if you do not select another type the already existing files in the folder will be overwritten In case there is an indicator displayed next to some of the file names it means that there has been a collision in the names and types of the files from the list In this case it will not be possible to save these files without a change of their name or type Legend for the displayed indicators will be displayed in the nformation part Files
106. ations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices Analog Video Source Remote Trigger Magnification Encoders Illuminators Annotations Insertion Mode Backup settings g Load Settings _F Configuration Wizard ok Cancel Tab Appearance and Layout On this tab you may select the program s color theme size of the thumbnails in the Thumbnail Browser and the appearance and layout of the Toolbar In the Color Theme part select one of the options and click the Change Theme button In the Thumbnail Browser part it is possible to select the Thumbnails Size by selection from the drop down menu 171 Main Program Window Options x 4 General Default Folders Color Theme Thumbnail Browser Thumbnails Size Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Display Toolbar Buttons with Captions Sizes Colors Grid V Lock Toolbar and Menus Toolbars and menus can be modified when unlocked Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Tlluminators Black Medium 140 x 105 px Silver Change Theme Toolba
107. aved as a part of the file and when you open it in the QuickPHOTO INDUSTRIAL 3 0 program next time they will be applied for this image 4 2 Scale Bar Inserting of the Calibrated Scale Bar For a basic idea about the dimensions you can insert into the current image a calibrated scale bar Option Measurements Insert Horizontal Scale Bar or the icon inserts into the right bottom corner a horizontal scale bar Option Measurements Insert Vertical Scale Bar or the icon inserts into the right upper corner of the image a vertical scale bar By clicking again on the corresponding icon you can remove the scale bar which was inserted into the image The value displayed above the scale bar shows its full length Scale bar is inserted in a layer above the image and if you change the display size of the image scale bar size and value will continuously recalculate Scale Bar Settings If you want to change the properties of the inserted scale bar click on the scale bar with the right mouse button A context menu with several choices will appear 117 Main Program Window Scale Bar Options Profiles Profile 1 Profile 2 Profile 3 Profile 4 Option Scale Bar Options opens an Options dialog box on the Scale Bar tab Edit gt Options Measurements Scale Bar This tab contains number of choices for type size and the appearance settings of the scale bar Created settings can be saved into one of
108. aying of some of the dialog boxes check its corresponding option on this tab 169 Main Program Window gy Display dialog box for change of active calibration upon a change of imaging device V Tf unchecked active calibration will be retained when changing imaging device g Display information about using calibration contained in the image when the required calibration is not found in Y the program during image opening pj Display confirmation dialog box for flattening of layers when saving an image as JPG or TIFF type with the Merge Objects with an Image option activated If unchecked layers will be automatically flattened g Display confirmation dialog box for automatic flattening of layers when saving an image as PNG or BMP type if unchecked layers will be automatically flattened gy Display confirmation dialog box about deleting all inserted objects when resizing an image V Tf unchecked all inserted objects will be automatically deleted gy Display confirmation dialog box when deleting all inserted objects V Tf unchecked all inserted objects will be automatically deleted y Display confirmation dialog box about discarding all changes when restoring original image Y Tf unchecked all changes will be automatically discarded y Display dialog box asking for exporting of unsaved measured values when an image that has been saved with flattened layers is being dosed If unchecked the values will be automatically
109. bar in the live view Font Size option enables to set the font size of scale bar s value Units Tab Options x Font Colors Scale Bar Units 4 Measurements Units Display Measured Angle Values Number of Decimal Places 0 Decimal Number of Decimal Places 2 In Degrees and Minutes On this tab you may choose units which will be used for measuring exporting micrometers or millimeters For the chosen units you may also choose the number of decimal places for displaying of the measured values Important The use of measuring accuracy in tenths of micrometers will only be relevant when the used microscope imaging device set provides such accuracy Way of expressing of the measured angle values can be set in the Display Measured Angle Values part Values can be displayed in Decimal form or In Degrees and Minutes 204 Control Panels of Imaging Devices Note Measured angle values will always be exported into Microsoft Excel program in decimal form irrespective of the settings of this option Measurements Tab Options x Font Colors Scale Bar M Units La Measurements Using the table below it is possible to set the measurands and labels for each measurement type Length Diameter Radius Perimeter Area Angle Distances Lengths 7 Cirde 7 Rectangle Polygon S S Ss lt S S S Ellipse Angle 7 Arc v v v Label L D R P A 6 Continuous Measureme
110. ble with Microsoft Windows 7 8 operating systems For now do not connect the IDS uEye camera to the computer If it is connected disconnect it Continue with steps nstallation of the QuickPHOTO INDUSTRIAL 3 0 Program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 and Installation Completion for more information see Installation Completion on page 18 Installation Installation of the PathScan Enabler IV Digital Microscope 1 For now do not connect the PathScan Enabler IV device to the computer If it is connected disconnect it 2 Continue with steps Installation of the QuickPHOTO INDUSTRIAL 3 0 Program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 and Installation Completion for more information see Installation Completion on page 18 Installation of the ARTRAY ARTCAM Digital Cameras 1 For now do not connect the ARTRAY ARTCAM camera to the computer If it is connected disconnect it 2 Continue with steps Installation of the QuickPHOTO INDUSTRIAL 3 0 Program for more information see Installation of the QuickPHOTO INDUSTRIAL 3 0 Program on page 17 and Installation Completion for more information see Installation Completion on page 18 1 2 Installation of OLYMPUS Digital Cameras E system CAMEDIA and SP series Installation of OLYMPUS cameras consists of two steps e In
111. ce Control Panel on the Second Monitor for Dual Monitor Workstations Do Not Show This Window Display Imaging Device Control Panel after Program Start up Annotations Appearance and Layout Properties of TWAIN Imaging Devices Control Panels Miscellaneous Close TWAIN Control Panel after Image Acquisition 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Bacup settings Gi Loadsettings F Configuration Wizard OK Cancel Tab Olympus SLR Consumer Cameras This tab is available only in case the support of OLYMPUS CAMEDIA and SP series or OLYMPUS E system SLR cameras has been installed and it is intended to set the device for display of life view from these cameras If you are using a device delivered with 183 Main Program Window the QuickPHOTO INDUSTRIAL 3 0 program it will be configured automatically after you see this tab When using a different device select this device from the Device drop down menu and select the norm of the video signal from the Video Standard menu In the Jnput menu the option Composite should be selected Checking of the Keep Images on the Memory Card option will cause that the acquired image will remain after transfer to a computer saved on the memory card this function is available only for CAMEDIA and SP
112. ce automatically Microsoft Windows XP Found New Hardware window will appear You will be asked whether you want to search for the driver on the internet Select No not this time Continue by clicking the Next button On the next tab leave marked the nstall the software automatically recommended option and click the Next button 20 Installation System will find appropriate device drivers and will ask you if you want to install them Click the Continue anyway button The device drivers will be installed Click the Finish button 4 4 Completing the Installation when Using the ARTRAY l ARTCAM Digital Cameras If you have restarted your computer sign in again to the administrator s account 2 Connect the ARTRAY ARTCAM camera to the USB 2 0 port of your computer using 3 the enclosed USB cable Continue with device drivers installation as follows Microsoft Windows Vista 7 8 Installation and commissioning of the camera device drivers will take place automatically Microsoft Windows XP Found New Hardware window will appear You will be asked whether you want to search for the driver on the internet Select No not this time Continue by clicking the Next button On the next tab leave marked the nstall the software automatically recommended option and click the Next button System will find appropriate device drivers and will ask you if you want to install them Click the Continue button C
113. cking the Next button New Report Template Wizard Processing Table 1 of 1 Define properties of the displayed table and dick the Next button Is the table displayed on the right hand side a mark which shows Table Found in Opened Pattern Document where the table of measured values from the QuickPHOTO INDUSTRIAL 3 0 program should be placed in the final report No this table is a part of the report and should be retained Yes link this table with measured values from image number i X Select measurands which should be displayed in the report 7 Dimensions Object Type Measurand Meas Nr Areas V Perimeters V Angles Objects Preview of the Table in the Final Report gt Hardness V Phases Select columns which should be displayed in the report V Object Type 7 Measurand 7 Meas Nr 7 Value V Units V Description lt Back Next gt Cancel 5 After elaboration of the tables is finished a page of the wizard containing a summary will be displayed After clicking the Finish button template will be created and saved into the QuickPHOTO INDUSTRIAL 3 0 Templates folder Note All report templates are saved in the QuickPHOTO INDUSTRIAL 3 0 Templates folder Possible backup restoration of templates can be done by simple copying of these files from to this folder 5 2 Creating a New Report Important Microsoft Word
114. clicking the 4 icon Drag your mouse while holding the left button over the area which shall be preserved the rest of the image will be cropped In the left upper corner of the selected area will appear a field showing its size Size is displayed in pixels and if there has been a calibration assigned also in length units 83 Main Program Window i e er SOT TEE EEO ere 9X 1445 1456 pixels 55 Peta hg 33 Y 33um be ee _ os He P i i ee he Tee a i i Clicking on the field displaying size will switch it to a mode in which it is possible to type in the values and to define precisely the desired size of the chosen area Width f on pixels Height 1426 Right from the field displaying size values is a lock icon If an open lock is displayed default state it is possible to enter both dimensions of the area independently After clicking on this icon a closed lock will be displayed This way the program will remember lock the ratio of the area s dimensions Then after you have entered one dimension the second one will automatically be calculated in the locked ratio If the image has an assigned calibration it is possible to after selection of units from the drop down menu on the right from the lock icon define the area dimensions in units of length New size can be confirmed by the OK button Recalculation of the area size will take place according to the entered values Field displaying the size ca
115. d Microscopes 46 Creating a New Standard Calibration 35 Opening Information about Calibrations 33 Setting an Active Calibration 58 Configuration Wizard 25 Control Panels of Imaging Devices 189 Analog Video Source 275 ARTRAY ARTCAM Digital Cameras 233 Canon EOS Digital SLR Cameras 217 IDS uEye Digital Cameras 221 Lumenera INFINITY Digital Cameras 189 Program for Olympus Consumer Digital Cameras 268 Olympus DP72 Digital Camera 255 Olympus E system Digital SLR Cameras 242 PathScan Enabler IV Digital Microscope 239 I Indication and Annotation of Interesting Areas Draw Arrow 92 Draw Circle 92 Draw Ellipse 92 Draw Freehand 93 Draw Line 91 Draw Polygon 92 Draw Rectangle 92 Insert Text Annotation 93 Initial Configuration of the Application 25 Installation 9 Adding the Support of a New Imaging Device 22 Adding the Support of Magnification Encoder or a PC Controlled Illuminator 23 ARTRAY ARTCAM Digital Cameras 1 AV USB 2 0 PRO Device 12 Canon EOS Digital SLR Cameras 10 Change of OLYMPUS Digital Camera Type CAMEDIA and SP series 23 Connecting the OLYMPUS Camera with the Computer 16 Device for Displaying of Life View 11 Dongle 17 315 Index Firmware Update OLYMPUS E 330 Digital SLR Camera 13 IDS uEye Digital Cameras 10 Installation Completion ARTRAY ARTCAM Digital Cameras 21 Installation Completion IDS uEye Digital Cameras 19 Installation C
116. d by clicking the right mouse button This way you may define more than 127 Main Program Window one region in the image This tool is active only for the second and third mode from the Measurement Area part Define Region Freehand Area 5 after you have activated this tool define one or more regions in the image using freehand drawing If you draw several discontinuous lines individual segments will automatically be connected with a straight line Clicking the right mouse button will close the region This way you may define more than one region in the image This tool is active only for the second and third mode from the Measurement Area part Delete Selected Regions X activation of this tool will delete regions selected using the Select Region tool This tool is active only for the second and third mode from the Measurement Area part Region Border Color Thickness Til left part of this icon is used for setting of the line s color of all regions Right part of this icon is used for setting of the line s thickness This tool is active only for the second and third mode from the Measurement Area part Important For work with regions for phase analysis you must always have the Phase Analysis dialog box open Note Regions for phase measuring are for clear differentiation from other types of objects in the image indicated with dashed lines Part Active Phase Selection In this part select the active phase which you want
117. device delivered with the QuickPHOTO INDUSTRIAL 3 0 program it will be configured automatically after you see this page When using a different device select this device from the Device drop down menu and select the norm of the video signal from the Video Standard menu In the Jnput menu the option Composite should be selected Checking of the Keep Images on the Memory Card option will cause that the acquired image will remain after transfer to a computer saved on the memory card this function is available only for CAMEDIA and SP series cameras If you leave this option unchecked recommended the acquired image will be after transfer to a computer from the camera s memory card automatically deleted To continue click the Next button Configuration Wizard QuickPHOTO OLYMPUS SLR Consumer Digital Cameras INDUSTRIAL In this step select a device video standard and input used for live view display from E 3 0 OLYMPUS SLR E System series and consumer digital cameras CAMEDIA and SP series In the first drop down box choose a device which will be used for live view display in the second one choose the video standard Then select the input type Device uF AV USB 2 0 Pro Video Standard Composite PALS E O S Video Default video standard for European cameras is PAL B To keep the images on a memory card after acquisition check the following box If unchecked the image will be deleted from the memory card immediat
118. discarded y Display dialog box asking for exporting of unsaved measured values when merging objects with an image V fattening layers If unchecked the values will be automatically discarded q Display dialog box asking for exporting of unsaved measured values when resizing an image If unchecked the values will be automatically discarded y Display dialog box asking for exporting of unsaved measured values when deleting all inserted objects wif unchecked the values will be automatically discarded q Display dialog box asking for exporting of unsaved measured values when restoring original image V Tf unchecked the values will be automatically discarded fey Backup Settings Ga Load Settings Configuration Wizard OK Cancel Tab Annotations On this tab you may set the mode of inserting annotations and drawings If the Continuous Inserting Mode option is active the selected tool for annotating or drawings will be active until its manual deactivation If you activate the Single Insertion Mode option selected tool will be after inserting of one object automatically deactivated 170 Main Program Window Options x 4 General Default Folders Image Names amp Saving Continuous Inserting Mode Selected tool remains active until deactivated Sound Language Single Insertion Mode Single object will be inserted upon tool activation Do Not Show This Window Annot
119. displayed In this dialog box choose from the Available Calibrations drop down menu calibration which you would 58 Calibration of the Program for Measurements like to set as active for the newly selected imaging device and confirm it by clicking the OK button Select Active Calibration for Lumenera INFINITY Digital Camera Select a calibration which should be used with selected imaging device and click the OK button If you dick the Cancel button currently used calibration will be retained Available Calibrations CCD Camera Upright Microscope Do not show this window again Active calibration will not be changed when switching to another imaging device next time 59 60 Calibration of the Program for Measurements Main Program Window Main Program Window of QuickPHOTO INDUSTRIAL 3 0 program is an image editor in which images are displayed right after they were acquired or opened This editor is equipped with functions for measuring editing and saving of images Main Program Window is designed for working with more than one image at the same time It is possible to display 1 2 or 4 images next to each other at the same time All opened images are displayed in the Thumbnail Browser The Navigator field makes orientation in the enlarged image easier The following functions for editing of images are available in the QuickPHOTO INDUSTRIAL 3 0 program crop clockwise and counter clockwise rotation rolling along th
120. dit an already inserted comment Statistics On the right hand side next to the table of measured values are displayed statistics calculated from the measured values Statistics are always calculated only from measurands of the 140 Main Program Window same type for example only dimensions perimeters and so on Selected measurand from which statistics should be calculated in case of more types of measurands measured in the image can be selected by selecting the desired row in the table Toolbar Buttons amp amp rs g m 4 ah r a Export Values Hide Undock Dimensions Perimeters Areas Angles Objects Phases Hardness Measured values displayed in the table can be exported into the Microsoft Excel file or ix a text file using the Export Values 2v s button A dialog box Export Measured Values will open after you click this button offering options for location name and type of the file Table of Measured Values can be to gain more working space hidden by the Hide hee button To display the hidden Table of Measured Values again use the E icon in the Toolbar activate the View Display Table of Measured Values option or use the F12 key shortcut If there is a greater number of measured values in the table it might be useful to for a better z clarity undock the Table of Measured Values as a separate window using the Undock rix button The Table of Measured Values will be displayed in a separate w
121. djustments before you begin with the phase analysis Above mentioned adjustments may have affect on the results of phase analysis Note You can in case of need convert a color image to a grey scale image using the function Image Grey Scale Phase Analysis function can be activated by the Measurements Phase Analysis option or by clicking the 4 icon In the right part of the monitor a Phase Analysis dialog box will appear 125 Main Program Window Caiculiat Ne Phase Propo 10 nEelatve to Whole Image Area Area of Defined Regions in the Image Whole Image Area Excluding Def Regions Overall Area of All Phases Description of the Phase Analysis Dialog Box Phase Analysis dialog box consists of the following parts e Measurement Area Tools e Active Phase Selection e Active Phase Controls e Preview 126 Main Program Window Overlapping of Phases Calculate the Phase Proportion Relative to Part Measurement Area In the Measurement Area part you may choose one of the three options 100 _ Use the Whole Image Area for Phase Analysis this option activates phase analysis in the whole image area Phase Analysis Inside Defined Region s of the Image j after you have activated this option you can define one or more regions in the image in which phase analysis will take place Remaining image area will not be considered for phase analysis Exclude Unw
122. dness Test x Test Type HBW s Impression Diameter d mm Ball Diameter D mm Mass m 3000 kg Time t 10 lt s Brinell Hardness HBW 2g HBW 0102 ar 7D 4D d OK Cancel Main Program Window Vickers Hardness Test Vickers microhardness test uses for determination of hardness impression of a regular tetrahedral pyramid of a known vertex angle between the opposite walls by a defined force into the tested material A testing machine the so called microhardness tester is used for this purpose Imprint can be scanned either directly by the microhardness tester with a camera output or by a microscope imaging device set and evaluated by the Vickers Hardness Test function After entering of values of the operating load and the duration time the value of the material s hardness will be calculated To evaluate the Vickers hardness test proceed as follows l 2 Acquire or open image of the imprint in the Display Window and assign it a magnification which was used to acquire this image Activate the Vickers Hardness Test function by the Measurements Hardness gt Vickers Hardness Test option or by clicking the icon Mouse cursor will change to a cross By clicking into the image place the cross so its center is precisely placed over one of the corners of the imprint Then place 3 more crosses in the same way on the remaining corners of the imprint After you have placed all four c
123. e Distance Between 2 Parallel Lines after you click this button function for measuring of a distance between two parallel lines will be activated By clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert the first line Then by clicking the left mouse button into the desired point insert the second parallel line Distance between both parallel lines will be measured Precisely horizontal or vertical line can be created if you hold the Shift key while inserting it Measure Circle Defined by Radius after you click this button function for measuring of a circle will be activated Then by clicking and dragging the mouse while holding the left mouse button select the circle s radius You can measure the circle s diameter perimeter and area 197 198 Control Panels of Imaging Devices Measure Circle Defined by Diameter after you click this button function for measuring of a circle will be activated Then by clicking and dragging the mouse while holding the left mouse button select the circle s diameter You can measure the circle s diameter perimeter and area Measure Circle Defined by 3 Points after you click this button function for measuring of a circle will be activated Then click the left mouse button gradually into three points in the live view through which the circle will be interspaced You can measure the circle s diameter
124. e Magnification Encoder 1 Connect the AC adapter and the connecting cable to the control unit and connect it to the computer 2 Run the QuickPHOTO INDUSTRIAL 3 0 program and activate Options of the program Edit Options In the Hardware part activate the Magnification Encoders tab In the Stereomicroscopes part check the Use Zoom Objective Encoder option and in the Control Unit drop down menu choose the control unit of the magnification encoder If you also have connected the magnification encoder for objectives choose it from the Objective Encoder Device drop down menu Correct function of the magnification encoder can be verified by clicking the Test Device button If case of successful verification of the device s functionality close the Options dialog box by clicking the OK button You will be asked to set the zooming unit to the 0 8 position to calibrate the zoom encoder Set the required zoom value and continue by clicking the OK button 52 Calibration of the Program for Measurements Note Only those control units whose support was chosen during program installation will appear in the list If your control unit is not available in the list exit the QuickPHOTO INDUSTRIAL 3 0 program and run the program installation from the installation CD again On page Installation Type Selection choose the Update Existing Installation Add Components option and on page Select Additional Components Magnification Encoders choose fr
125. e axes resize brightness adjustment contrast adjustment color balance and gamma correction adjustment histogram adjustment using filters sharpen blur negative grey scale median filter black and white balance adding text annotations to an image labeling with drawings tools line arrow rectangle circle ellipse polygon freehand drawing QuickPHOTO INDUSTRIAL 3 0 program is also equipped with a number of measuring functions inserting a calibrated scale bar with the option to define the look and position of the scale bar distance measurement line segment polyline distance between two parallel lines measuring of perimeters and areas of objects tools ellipse rectangle circle polygon measuring of circle diameters angle measurement arbitrary angle angle from the horizontal axis angle from the vertical axis angle defined by two line segments radius angle and length measurement of an arc display of the calibrated grid orthogonal circular 61 Main Program Window e counting of objects e Brinell hardness test according to the norm EN ISO 6505 1 1999 e Vickers microhardness test according to the norm EN ISO 4516 2002 e phase analysis All inserted objects drawings annotations measurements are displayed in a separate layer above the image and it is possible to modify change of the line s color and thickness change of size change of position delete or hide them at any time It is also po
126. e camera on Note In the camera s menu make sure the Live View Boost function is turned off OLYMPUS E 330 E 510 E 410 SLR Digital Cameras In the camera s menu in the Settings 2 menu symbol of a key with the number 2 move the cursor to the USB MODE item Press the right arrow button and select the CONTROL option Confirm by pressing the OK button OLYMPUS E 3 E 30 E 620 E 520 E 450 E 420 SLR Digital Cameras In the camera s menu in the Settings 1 menu symbol of a key with the number 1 or for the type E 620 the User Menu cogwheel symbol move the cursor to the DISP sound PC item and press the right arrow button Move the cursor to the USB MODE item press the right arrow button and select the CONTROL option Confirm by pressing the OK button OLYMPUS E 600 SLR Digital Camera In the camera s menu in the Settings menu symbol of a key move the cursor to the MENU DISPLAY item and press the right arrow button Using the arrow buttons set the value ON Confirm by pressing the OK button Further on in the camera s menu in the User Menu cogwheels symbol move the cursor to the DISP PC item and Installation press the right arrow button Move the cursor to the USB MODE item press the right arrow button and select the CONTROL option Confirm by pressing the OK button lt OLYMPUS CAMEDIA and SP Series Digital Cameras Make sure the USB cable as well as the TV output cable is disconnected from the camera
127. e images being captured and the set exposure time of the active imaging device If you set an interval shorter than is the time necessary for image capturing and its transfer to the computer you will be warned about this fact and the time interval for image capturing will be set at a minimum applicable value Location of the Captured Images Location of the captured images can be selected in the Store Images part There are two options available e in the Main Program Window images will be displayed after capturing in the Main Program Window this option is suitable for capturing of a smaller number of images e in Folder this option is recommended for capturing of larger number of images Images will be after capturing automatically saved into the selected folder and they will not be displayed in the Main Program Window To select the folder click the button 284 Modules and select the desired location Enter the file name of the sequence being captured into the File Name field Individual images will be automatically differentiated using the five digit serial number attached after the selected name In the bottom part of the dialog box there are displayed information about the process of time lapse image capturing In the Time until Next Capturing part the time remaining until next image capturing will always be displayed Further you will have information about how many images have already been captured in the current se
128. e in a similar manner If you have not installed or activated the objective encoder these pages will not be displayed at all Continue by clicking the Next button Note If you have the objective encoder active you must assign the corresponding nosepiece position to at least the first objective The second objective may remain unassigned Calibration of the Program for Measurements Add Calibration CCD Camera Stereomicroscope x First Objective Position and Magnification Assignment Values for Assignment 1 In case your imaging device is connected to trinocular tube or right sided photo adapter rotate the Nosepiece Position nosepiece to position number 2 In case of connection to left sided photo adapter rotate the nosepiece to position number 3 2 Select corresponding magnification from the Objective Magnification drop down menu 3 Click the Assign Values button Ni iece Position osepiece Position 4 Click the Next button to continue Objective Magnification lt Back Next gt Cancel Dialog box named Table of magnifications Calibrations Calibration Name which will have in the Table of Calibration Values part filled in the zoom magnifications will open If you have also activated the objective encoder magnifications of the used objectives and their corresponding encoder positions will also be filled in There will now be also active the Change Objective P
129. e left mouse button into the starting point and then the endpoint a line may be drawn Precisely horizontal or vertical line may be drawn if you hold the Shift key while drawing it Draw Arrow Tool Draw Arrow can be activated by the Annotations Arrow option or by clicking the icon Then by clicking into the image and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the starting point and then into the endpoint an arrow may be drawn The arrowhead will be placed to the endpoint Precisely horizontal or vertical arrow may be drawn if you hold the Shift key while drawing it Draw Rectangle Tool Draw Rectangle is activated by the Annotations Rectangle option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw a rectangle Draw Circle Tool Draw Circle is activated by the Annotations Circle option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw a circle Draw Ellipse Tool Draw Ellipse is activated by the Annotations Ellipse option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw an ellipse It is possible to rotate the ellipse by dragging the green grasp point displayed inside the ellipse Draw Polygon Tool Draw Polygon is activ
130. e middle of selected objects of the ellipse rectangle and circle types there is a cross Using this cross you may also move these objects in the above mentioned way Use a similar process to move text annotations In the ellipse there is also a green grasp point connected by a dash line with its center Using this point you can rotate the ellipse any way you wish To change the size shape of the selected object proceed as follows move the mouse cursor to one of the green grasp points Cursor will change to u By clicking on the grasp point and by dragging the mouse while holding the left mouse button you can change the size shape of the object Deleting of Inserted Objects and Text Annotations In order to edit inserted objects select them first for more information see Selection of the Inserted Objects for Editing on page 95 96 Main Program Window To delete a selected object from the image press the Del key activate the Edit Delete Selected Objects option or click the icon To delete all not only selected objects inserted objects from the image activate the Edit Delete All Objects option or click the Z icon 3 4 Image Saving Information about Work with Layers Program QuickPHOTO INDUSTRIAL 3 0 uses for work with inserted objects drawings text annotations measurements grid scale bar an independent layer above the image Thanks to this function it is possible to edit hide or delete al
131. e mouse while holding the left mouse button insert a rectangle You can measure the rectangle s perimeter and area Control Panels of Imaging Devices Measure Polygon after you click this button function for measuring of a polygon will be activated Then by clicking the left mouse button into the selected point in the live view insert the first vertex of the polygon Repeated clicking of the left mouse button will define other vertexes of the polygon Precisely horizontal or vertical edge can be created if you while inserting the vertex hold the Shift key To finish inserting of the polygon click the right mouse button the last vertex will be connected with the first one and a polygon will be created You can measure the polygon s perimeter and area Note If the edges of the inserted polygon will cross each other somewhere the value of the calculated area will equal 0 Only the polygon s perimeter will be calculated Measure Polyline after you click this button function for measuring of a polyline will be activated Then by clicking the left mouse button into the selected point in the live view insert the beginning point of the polyline Repeated clicking of the left mouse button will define the break points Precisely horizontal or vertical line can be created if you hold the Shift key while inserting it To finish inserting of the polyline click the right mouse button Length of the polyline will be measured Measur
132. e name tab Font Size 12 6 7 8 9 10 Use Custom Color for All Measurements Transparent Custom Color x Tab Grid On this tab it is possible to select the default parameters of the grid It is possible to set the default Spacing and Default Grid Line Parameters Line Color and Line Thickness 178 Main Program Window Options 4 General Default Folders Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors x Default Grid Line Parameters Line Thickness 3 pixels E Line Color Default Orthogonal Grid Spacing 0 04 2 mm Default Circular Grid Spacing Spacing 0 04 lt mm Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices Analog Video Source Remote Trigger Magnification Encoders Illuminators gt Load Settings J Configuration Wizard fey Backup Settings Tab Measurands This tab serves for selection of measurands which will be measured by individual measuring tools and for text labeling of individual measurands Measurands to be measured should be checked To change the label rewrite the appropriate field in the Label row Display Measurement Numbers in the Image option determines whether each measured value in the image will have the appropriat
133. e saved as BMP bmp or PNG png type These types do not support saving of layers Inserted objects will be merged with image Saving of Images When Exiting the Program When exiting the QuickPHOTO INDUSTRIAL 3 0 program you will be asked whether you wish to save the not yet saved images Option Save called from the displayed message window works the same way as the option File Save All 107 Main Program Window 3 5 Image Printing Program QuickPHOTO INDUSTRIAL 3 0 enables to print 1 4 images next to each other on one page Select images for printing max 4 in the Thumbnail Browser and activate the File Print option or click the icon Function Print can also be activated for a particular image by clicking the Print 8 button in the controls of the corresponding thumbnail Note If you select more than 4 images in the Thumbnail Browser and you activate the Print function only the first four images of the selection will be printed If there are 2 or 4 images displayed next to each other in the Display Window and you have selected only one thumbnail in the Thumbnail Browser first a window Selecting the Source of Images for Print will open when activating the print function 108 Main Program Window Selecting the Source of Images for Print x Source of Images for Print Selected Thumbnail Layout of Displayed Images Option for print of the image selected in Option for print of a layout of
134. e serial measuring number displayed by it 179 Main Program Window Using the table below it is possible to set the measurands and labels for each measurement type Default Folders All these settings represent default values for all new images Parameters can be adjusted for particular images in the t Image Names amp Saving Table of Measured Values in the Measurement Settings tab image name after clicking the Measurands button g Do Not Show This Window Length Diameter Radius Perimeter Area Angle Annotations Distances Lengths 7 3715 8 5 8 Analog Video Source Display Measurement Numbers in the Image Enabled fel Backup Settings Ga Load Settings Configuration Wizard OK Cancel Tab Hardness On this tab it is possible to set the default values for the Brinell hardness test and for the Vickers microhardness test 180 Main Program Window Options x 4 General Default Folders Default Values for Hardness Measurements Image Names amp Saving Brinell Hardness Test Vickers Hardness Test Sound Language Test Type HBW v Mass m 0 01 kg Do Not Show This Window Ball Diameter D 10 to Time t 10 e s Annotations Appearance and Layout Mass m 3000 kg Miscellaneous 4 Measurements Time t 10 lt En Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices Analog
135. e so called white point right triangle Set values are displayed in editable fields under the histogram In the bottom part of the window there are image previews before and after changes have been made Buttons and between both previews are there for the change of display size of both previews After previews have been zoomed in it is possible to by dragging the mouse while holding the left mouse button move both previews Default Values button in the bottom part of the window will bring all settings in this dialog box to the original state Changes made can be applied to the whole image by clicking the OK button 3 3 Indication and Annotation of Interesting Areas Program QuickPHOTO INDUSTRIAL 3 0 is equipped with functions for indication of interesting areas in the image using drawings and text labels You may use the following tools e Text Annotation e Draw Line e Draw Arrow e Draw Rectangle e Draw Circle e Draw Ellipse Draw Polygon e Draw Freehand The use of individual tools is described in the following paragraphs 90 Main Program Window Note The use of the above mentioned tools can be canceled anytime by the right mouse button click or by the Esc key Annotations Insertion Mode Program QuickPHOTO INDUSTRIAL 3 0 offers two modes of annotations inserting Make your selection in Options of the program in section General at the Annotations tab Edit Options General Annotations in the
136. e the specimen back in and set the desired exposure time 7 In the Correction Level part click the Preview in Live View button According to the need you may using the Correction Level slider adjust the correction Reflected Light 1 Set the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Place a white sheet under the microscope for example a sheet of white paper Do not focus the microscope and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Auto WB button automatic white balance On the Jmage tab set the desired image size 5 Switch to the Shading Correction tab and select the Enabled option In the umination Type part select the Reflected option Then in the Calibration Mask part click the Capture Mask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in focus the microscope and set the desired exposure time 7 In the Correction Level part click the Preview in Live View button According to the need you may using the Correction Level slider adjust the correction All acquired images will now be automatically adjusted using the Shading Correction function until the function is di
137. easier to focus the selected area of the image To use this function proceed as follows 1 Using the focus knob roughly focus the microscope Activate the Focus region tool Move the cursor into the live view and insert the frame in the live view by dragging the mouse while holding the left mouse button to a place where you want to do the focusing 256 Control Panels of Imaging Devices 2 Focus indicator will be displayed next to the frame Blue strip represents the current focus value and the red guideline maximum reached focus value Both values are at the same level at the beginning 3 Ifyou start rotating the microscope focus knob now the blue strip will start to decline respectively rise according to the fact whether the focus level in the framed area will decrease or rise Select such a direction so the strip starts to rise Continue rotating the focus knob until the blue strip starts to decline Red guideline will remain on the position of the maximum reached focus value Gently rotate the focus knob in the opposite direction now achieve that the blue strip reaches as closely as possible to the red guideline in this position image of the selected area will be as sharp as possible You can use numerical values in a similar way for focusing when activating the Focus region tool by the Numbers icon In this mode both focus level values are displayed in the current value maximal reached value form Focus region reset in
138. easured Values _ 9fMessuredValues_ Dutton in the Toolbar enables to hide or display again the Table of Measured Values If the Table of Measured Values is hidden a Table of Measured Values 9fMeasured vates tab will appear in the bottom part of the control panel If you move the mouse over this tab the table will be temporarily displayed If you move the mouse to a different area the table will automatically be hidden again It is possible to display the table permanently also by clicking the button in its right upper corner C Table of Measured Values Contains Unsaved Values Object Type Measurand Meas Nr Value Units Description Measurand Length eng Line Segment Length 1 261 um Number of Measurements 5 Line Segment Length 185 um Minimal Value 106 Line Segment Length 158 pm Maximal Value 261 Line Segment Length 139 um Average Value Line Segment Length 106 pm Standard Deviation 4 ix X x Export Values Clear All Measured Values Delete Selected Value s In the bottom part of the Zable of Measured Values there is a toolbar with buttons which are enabled immediately after the first done measurement 200 Control Panels of Imaging Devices iy Export Values this button enables to export all measured values from the ExportWalues Table of Measured Values to the Microsoft Excel file or text file Important For export of measured values to the file of the xls format it is necessary to have the
139. eeeeeeenenenees 288 Sy DEED FOCUS Optional reenen ataa Sar hone baguette e metal 293 A RECORD TT 0pti ona oc ienna EE op aa EE TEAN EE 294 FLUOR OPHONAL riaa ei i A A N 294 Adding of Modules and Upgrading 0 e cece cence cence cece eee e eee eeeeneneeees ease en 295 FIST OB Key SMOLIC WIS nania e cama eea a tareneassas 297 Troubleshoot FA Oye ca sek cite ir ET TATAE EO OTA ET eee alent 301 BY Ce MN REJUIE MENS seg Soe ah ail ete eto chet td ane ae dla Neti N 313 MINOX PEE EEE E E cas tence AELE Acie cs A E E E E ETA 315 QuickPHOTO INDUSTRIAL 3 0 Introduction QuickPHOTO INDUSTRIAL 3 0 program is designed for acquiring of digital images from microscopes equipped with digital cameras for editing and saving of acquired images and measurements This program is designed for use especially in the industry and materials research QuickPHOTO INDUSTRIAL 3 0 program is equipped with a number of measuring functions for example measuring of lengths perimeters areas angles distances of parallel lines counting of objects phase analysis and measuring of hardness and microhardness Q QuickPHOTO INDUSTRIAL 3 0 samplel jpg File Edit Image View Measurements Annotations Imaging Devices Modules Reports Window Help 2 WS e B B t ovective 0 5 Zomos 2 OUC Y H S27e BER ODM i A aS ems sample 1 jpg 1194 x 973 E Table of Measured Values s
140. een tow meanenne E 114 A 1 Preparing tor Measuring 2 sauce nos24Ginlnanaatee aig NEA EEE E TAE 115 Dk SO ANS BTI Sh sate E A ua E ela ioe A tetet bie 4 3 Standard Measuring Tools occ ccceccteiccdiseencedatedadieestecceee tedden dieneceted 118 4 4 Displaying a Grid of Defined Dimensions 0c cccc cece eee eeee ence eee 123 Ae PASE GAT ANY SS aeae a ah en T ETRE E 125 Aos adne Ss FeS aenn a leratdeencolnen ea 133 4 7 Editing of the Measuring Objects c ccc ccc cece ec eee eeee eee eeeeseeeneeaens 136 4 8 Work with the Table of Measured Values cc cece ec eeeseeeaeeneenens 139 4 9 Exporting Measured Value ecicswncssavee caschwsanssiawieles ence nibivin ce tecianneeaes 144 IRPO eii ON wiaberte die wo arated a a erated teas ash ata teal cnatrntaGiedAcpureltones 148 5l Creating New Report Template reterii a a aa 149 5 2 Creatine a NEW REDON merokoitanetiarninie n i a a aan hae 155 QuickPHOTO INDUSTRIAL 3 0 So Report Template Mana Cer iernare A E 158 OP OUMEE FUNC MOMS esere E T TE T eet 159 Gils imase Inom oi eruen a e ent A 159 6 2 Compose New Image from Layout of Displayed Images 161 OS ONCES HOW ataken Meneueslab ena hteubeund halt e 163 64 Display in Bull Screen Mode vevsc ce wes cimnsceeetarenciadnenceunass ravens 164 POPIO nner tne ee Dee Reena ee Oe eee ea ene Dneeee esa eaten an mn A ton eee ree 164 Ole e CHO CICMCbA aiaa ihre aes saanuss team a iaahead eat ae Meaausd
141. efault Black Balance Settings clicking this button sets all values of the sliders in the Black Balance part to the default values 209 Control Panels of Imaging Devices Shading Correction Tab This tab contains control elements of the shading correction function This function may be used to remove deficiencies in the image caused by inhomogeneous illumination removal of the darker corners of the image or by dust on the sensor go Shading Correction Disabled Enabled Illumination Type Reflected Transmitted f Calibration Mask 2592x1944 Shading Correction this option is used for turning the Disabled Shading Correction function on off Enabled Illumination Type in dependence on the type of the used Reflected illumination select Reflected or Transmitted option The SS correction method is different for each illumination type 210 Control Panels of Imaging Devices Calibration Mask if you click the Capture Mask button an nae image which will be used as a calibration mask for shading correction function will be acquired In the field above the button will be displayed the preview of the captured mask and information about its size Correction Level using this slider you may set the strength with which will be the shading correction applied If you click the Preview in Live View button you will see the effect of the shading correction function reflecting the current settings Usi
142. els can be piezo shifted during image acquisition to obtain a maximum effective resolution of 12 8 million pixels Peltier cooling of the chip to the temperature 10 C lower than is the temperature of the environment provides significant reduction of noise level and high sensitivity OLYMPUS DP72 camera is suitable for low signal intensity fluorescence microscopy 255 Control Panels of Imaging Devices The control elements are located in the toolbar and on six tabs OLYMPUS DP72 camera control panel is intended to control the camera functions live view displaying and image acquisition It consists of a toolbar six tabs and the live view Toolbar The toolbar is located above the live view The toolbar contains tools that control the camera s parameters and adjust the parameters of the live view Standard view displays the live view in a standard mode disables a zoomed view if active Zoomed view activate this tool and click in the live view to zoom the live view to 100 This option does not influence the acquired images This button is inactive when the resolution of the live view is set to 1360 x 1024 pixels Rotate by 180 rotates the image by 180 Z Flip vertically flips the live view along the horizontal axis Flip horizontally mirrors the live view along the vertical axis A Color Grey scale mode switches between color and grey scale mode Focus region Bar graph ES Numbers E this function makes it
143. ely after transfer to a PE Keep Images on the Memory Card Available for CAMEDIA and SP Series Only Analog Video Sources This page will appear only if the support of analog video sources has been installed and it is intended to set the digitalization device for acquiring images from these sources If you 26 Initial Configuration of the Application are using a digitalization device delivered with the QuickPHOTO INDUSTRIAL 3 0 program it will be configured automatically after you see this page When using a different digitalization device select the device from the Device drop down menu and select the norm of the video signal from the Video Standard menu In the Input part you may select the desired type of the used connector To continue click the Next button Configuration Wizard QuickPHOTO Analog Video Sources INDUSTRIAL In this step select the device video standard and input used for analog video sources 3 0 In the first drop down box choose a device which will be used for analog sources in the second one choose the video standard Then select the input type Device AV USB 2 0 Pro Video Standard PALS eS S Video CY Composite Default Folders Next page of the wizard enables to set the folders which will be offered as default when saving images exporting measured values and saving reports This way you will not have to browse the whole directory structure of the computer to select the desired
144. ement Settings sample5 jpg Object Type Measurand Meas Nr Value Mass kg Time s D mm difum dimm d2 um HBS Hardness 3 310 3000 10 10 3 456 Tab Measurement Settings Image Name Measurement Settings Image Name tab contains control elements for changes of settings for measuring in the current image displayed in the Display Window Control elements on this tab are activated after the first measuring has been done in the image or after inserting of the scale bar 142 Main Program Window E Table of Measured Values samplei jpg Measurement Settings sample 1 jpg Here measurement settings for the current image can be modified Labels with Measured Values Font Size of Scale Bar Value Font Size 12 X Font Size 12 X Display Measurement Numbers in the Image Yes z Background Color Line Thickness Transparent Custom Color Line Color Measurands Note All changes of settings done on this tab apply only to the current image Other images opened in the program will remain without changes If you want to change the settings for all new images use settings in Options of the program on the tabs Sizes Colors and Measurands Edit Options Measurements Sizes Colors Measurands In the Labels with Measured Values part you may change Font Size set the Display Measurement Numbers in the Image option and select the Background Color of labels with measured v
145. ements Calibrate New Magnification Calibration CCD Camera Stereomicroscope X 1 Magnification Selection Rotate the nosepiece to position 2 or 6 and set zoom magnification intended for calibration Magnification for Calibration Current Objective Magnification Current Objective Position Zoom Magnification Changer Magnification Recalibrate Zoom Encoder Note Recalibrate Zoom Encoder button 1s intended for correction of the zoom encoder s calibration Use this button only if the zoom encoder s values are not correct Table of Magnification after a correctly done calibration might for stereomicroscope with the zoom and objective magnification encoder look for example like this one Table of Magnifications Calibration CCD Camera Stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification SZX 16 Recalibrate Selected Magnification Objective Position 0 7 0 8 1 1 25 1 6 2 2 5 3 2 0 5 2 39 2477 33 8742 27 0481 21 5851 16 8185 13 4737 10 7496 8 4021 Semmndeedeateatinien 1 6 19 8012 17 0985 13 6741 10 9493 8 5236 6 8474 5 4373 4 2478 Remove All Magnifications Change Objective Positions Graph of Calibration Values 3 kei c ral Z a o ae Zoom Magnification OK 57 Calibration of the Program for Measurements 4 Setting an Active Calibration If you are
146. endently adjust the red green and blue channel Adjust Histogram opens a dialog box for the histogram adjustment Object Line Color Thickness is used for a change of color and thickness of the lines of inserted objects Left part of the icon enables a change of color right part with an arrow enables change of the line s thickness Select is used for selection of inserted objects Delete Selected Objects deletes objects selected with the Select tool Del Delete All Objects deletes all objects inserted in the image 75 Main Program Window a nsert Text Annotation activates a dialog box for inserting of a text annotation Draw Line activates a tool for drawing of a line Draw Arrow activates a tool for drawing of an arrow m Draw Rectangle activates a tool for drawing of a rectangle Draw Circle activates a tool for drawing of a circle lt gt Draw Ellipse activates a tool for drawing of an ellipse lt Draw Polygon activates a tool for drawing of a polygon g Draw Freehand activates a tool for freehand drawing 1 3 Thumbnail Browser Thumbnails of the opened images are displayed in the Thumbnail Browser together with their names and sizes Image opened in the Thumbnail Browser can be displayed in the Display Window by clicking the left mouse button on the thumbnail or by dragging of the thumbnail drag and drop into the Display Window If you have set a display of more than one image next to each other in
147. ension and Extension Lines and Leaders part it 1s possible to set the color and thickness of these lines In the Font Color of Measured Values part you may set two modes for the font color of the measured values Cycle Colors in the Following Order option will cause that individual measurements performed in the image will cyclically have font colors assigned in the displayed order There are 10 colors set on the list which can be changed by the user To change some of the colors make its selection click the Change button and select a new color from the opened color palette If you activate the Use Custom Color for All Measurements option all measurements will have the same font color which can be selected after clicking the Change button Background of Labels with Measured Values part provides following options Transparent or Custom Color In the Font Size of Measured Values Displayed in Images part it is possible to select the font size which will have the measured values displayed in the image 177 Main Program Window Options Appearance and Layout OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Tluminators Gl Badup settings g Load Settings Configuration Wizard All settings on this tab represent default values valid for all new images Some of these parameters may be adjusted for particular images in the Table of Measured Values in the Measurement Settings mag
148. ent the so called pattern document Creating of a template consists of two steps 1 Creating of a pattern document in the Microsoft Word 2 Creating of a report template in the QuickPHOTO INDUSTRIAL 3 0 program based on the pattern document 1 Creating of a Pattern Document in the Microsoft Word First step in creating of a new template is creating of a pattern document in the Document Word 97 2003 doc format which will define the layout and structure of the report template This document may contain a header with texts and logos images tables and texts Create a new pattern document in the Microsoft Word program which will correspond with your layout and structure requirements for the resultant report according to the following rules Pattern document should contain e at least one image in whose place in the report will be placed an image from the QuickPHOTO INDUSTRIAL 3 0 program e at least one table in whose place in the report will be exported a table of the measured values Images inserted to the pattern document may have two different purposes They may be e images which are supposed to be in an unchanged form a part of the resultant report for example a company logo 149 Main Program Window e substitute images which will in the template represent the so called marks which means they will define the location and size of the images which will be exported to the report from the QuickPHOT
149. ently rotate the focus knob in the opposite direction now achieve that the blue strip reaches as closely as possible to the red guideline in this position image of the selected area will be as sharp as possible You can use numerical values in a similar way for focusing Note Focus level is measured relatively That means the indicator shows whether the focus value is rising or declining towards the default value which is calculated after the frame is positioned If the focus value exceeds the indicator s range it is initialized again to about three quarters of the range That does not mean that the focus value would decline rapidly If you continue rotating the knob in the same direction and the strip rises again it means that the focus level continues to rise Reset button initializes the focus indicator again into the default state That means the maximum reached value of focus level is set along with the current value approximately to three quarters of the range Using the Display Hide button you can hide or display the frame of the focus region Note If you relocate or change the size of the focus region automatic reset of the focus indicator will take place see Reset button Black Balance using this function you may adjust the black balance By clicking the Region BB button and successive dragging of the mouse in the live view while holding the left F Region BB Default BB 194 Control Panels
150. erimeter and area of a rectangle Measure Circle Tool Measure Circle can be activated be one of the options from Measurements Circle menu or by clicking on the arrow on the right from the icon and by selecting the corresponding type of measurement from the displayed menu There are following options available 119 Main Program Window Circle Defined by Radius by clicking and dragging the mouse while holding the left mouse button you can define the radius of the measuring circle Circle Defined by Diameter by clicking and dragging the mouse while holding the left mouse button you can define the diameter of the measuring circle Circle Defined by 3 Points click the left mouse button gradually on three points in the image through which the measuring circle will be interspaced You can measure a diameter perimeter and area of a circle Measure Polygon Tool Measure Polygon can be activated by the Measurements Polygon option or by clicking the icon Then by clicking the left mouse button on a chosen point in the image insert the first polygon s apex Repeated clicking of the left mouse button will define further apexes of the polygon Precisely horizontal or vertical edge can be created if you hold the Shift key while inserting the apex To finish inserting of a polygon click the right mouse button the last apex will be connected with the first one and a polygon will be closed You can me
151. es OLYMPUS E 3 E 30 E 620 E 520 E 450 E 420 E 510 E 410 Digital SLR Cameras These cameras can be used from the standard user accounts in the Microsoft Windows XP Vista 7 8 operating systems e OLYMPUS CAMEDIA and SP Series Consumer Digital Cameras These cameras can be used from standard user accounts in the 32bit versions of Microsoft Windows XP Vista 7 8 operating systems To use those cameras in the 64bit versions of Microsoft Windows 7 8 operating systems it is necessary to use the account with administrator s privileges Note To run the program with administrator s privileges click the right mouse button on the program shortcut and select the Run as administrator option 304 Troubleshooting FAQ OLYMPUS E 330 Digital SLR Camera Important OLYMPUS E 330 Digital SLR Camera cannot be controlled from the Microsoft Windows Vista 7 8 operating systems In order to control it from the Microsoft Windows XP operating system it 1s necessary to use an account with administrator s privileges OLYMPUS E 330 digital SLR camera cannot be connected by the QuickPHOTO INDUSTRIAL 3 0 program How to solve this problem l Make sure the camera is turned on and switched to the PC CONTROL mode For more information see the section called Installation of OLYMPUS Digital Camera on page 13 Make sure your Microsoft Windows XP operating system has installed the Service
152. es not have any USB 2 0 ports install an additional USB 2 0 controller into your computer Make sure the performance of your computer corresponds with the minimal system requirements listed in the program specifications ARTRAY ARTCAM Digital Cameras The camera s device drivers cannot be installed Make sure the camera is connected to the USB 2 0 port of your computer Do not connect the camera to the USB hub 309 Troubleshooting FAQ Live view does not display or it is broken The camera is most likely having a compatibility issue with the USB controller Do not use an extension USB cable for connecting the camera Use an additional USB 2 0 controller for connecting the camera Disconnecting of the camera takes place after running of the control panel When using automatic exposure mode make sure the specimen in the camera s field of view is constantly sufficiently illuminated IDS uEye Digital Cameras When running the camera s control panel an error message about unsuccessful attempt to connect the digital camera appears re Make sure the camera is connected to the USB 2 0 port of your computer 2 Make sure the LED on the top part of the camera is light up with a green light if the 310 camera is equipped with such a LED If the LED is not light up or if the red light is on the camera s drivers have not most likely been installed properly In this case reinstall the camera s drivers Proceed as follows
153. es in seconds Default delay is set at three seconds If the Terminate Slideshow after the Last Image Display option is not checked the last image of the presentation will remain displayed on the monitor Maximum image size which can be opened in the QuickPHOTO INDUSTRIAL 3 0 program or which can be acquired is set in the Maximal Image Size part Default setting is 32 megapixels If you want to work with larger images change the settings of this parameter Important Opening of images larger than 32 megapixels can in older operating systems such as Windows XP cause unexpected behavior of the QuickPHOTO INDUSTRIAL 3 0 program Options 4 General Default Folders pE Image Names amp Saving Delay Between Images 3 lex V Terminate Slideshow after the Last Image Display Do Not Show This Window Annotations Maximal Image Size Appearance ai and Layout Choose the maximum size of images that can be opened in the program The default setting is for compatibility with Miscellaneous older operating systems 32 Megapixels Larger images can cause unexpected behavior of the program 4 Measurements Units Scale Bar Maximal Image Size in Megapixels 32 Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Bacup settings g Load Settings
154. evice Drivers Installation part leave checked the options for installation of the drivers On page Select Additional Tasks File Types Associations it is possible to set association of the QuickPHOTO INDUSTRIAL 3 0 program for opening of files of the BMP JPEG PNG or TIFF types If you check one or more of the mentioned options the QuickPHOTO INDUSTRIAL 3 0 program will always be used for opening of the corresponding file types If you have selected on page Select Components Imaging Devices the support of IDS uEye digital cameras installation wizard for IDS uEye cameras will open during installation Continue by clicking the Next button and click the nstall driver button Select the 3 USB option and continue according to the wizard s guidelines Finish the installation by clicking the Finish button When prompted restart your computer 18 4 Installation Completion 4 1 Completing the Installation when Using the Lumenera INFINITY Digital Cameras If you have restarted your computer sign in again to the administrator s account Connect the Lumenera INFINITY camera to the USB 2 0 port of your computer using the enclosed USB cable Continue with device drivers installation as follows e Microsoft Windows Vista 7 8 Installation Installation and commissioning of the camera device drivers will take place automatically Microsoft Windows XP Found New Hardware window will appear You will be asked w
155. example HDD DVD CD ROM connected network drives flash memory and so on Ctrl O qj Save saves an image images selected in the Thumbnail Browser Ctrl S amp Print initiates printing of selected images Ctrl P amp Image Information displays information about the current image Ctrl I Undo undoes the last performed image editing Ctrl Z ca Redo does again the last undone image editing Ctrl Y Copy inserts a selected area or a whole image displayed in the Display Window into a clipboard Ctrl C 71 Main Program Window g Paste inserts the clipboard content as a new image Ctrl V Select region after clicking on this icon you may select an area of the image by clicking and dragging the mouse while holding the left mouse button Objective Objective drop down menu for the choice of the objective which was used to acquire the image It is used to assign the correct calibration for measurements a Zoom drop down menu for the choice of zooming unit magnification changer magnification which was used for acquiring of the image It is used for assigning of the correct calibration for measurements If you are not using either the zooming unit or the magnification changer value 1 will automatically be filled in this field after you choose an objective Insert Horizontal Scale Bar inserts a calibrated horizontal scale bar into the right bottom corner of the image Insert Vertical Scale Bar
156. field the name of all selected images will be changed at the same time and the Numbering function will also be activated This function will ensure distinction of individual images Changes will immediately appear in Thumbnails of Images for Saving part If you want to restore the original names of the selected images click the Restore button Save as Type drop down menu enables to select the type format of the images selected in the Thumbnails of Images for Saving part If you leave the default option Original Type active images will be saved in the same format in which they were opened acquired in the program If you choose one of the available types from this menu the selected type will be set to the images selected in the Thumbnails of Images for Saving part If you choose the JPEG type indicator of the set JPEG quality will also be displayed by the corresponding thumbnails for example JPEG Quality 95 and at the same time JPEG Quality link will be made available right from the Save as Type drop down menu This link will open a window in which it will be possible to set the desired quality of the JPEG type images File Name and Type File Name sample 1 Restore Save as Type Original Type JPEG Quality 106 Main Program Window Part Additional File Name Tags In the part Additional File Name Tags there are two buttons Button Date will add the current date to the image names selected in the Thumbnails of Images for Sav
157. file proceed according to the following steps 1 Ifyou intend to create a video file from images currently opened in the QuickPHOTO INDUSTRIAL 3 0 program select them in the Thumbnail Browser 288 Modules Run the Time Lapse Video module from the Modules Time Lapse Video menu or click the icon in the Toolbar The Time Lapse Video wizard will open On the Selection of Image Source tab select the source of images from which the resulting video file is supposed to be created There are three options available Process Images Selected in the Thumbnail Browser select this option if the video file is going to be created from a smaller number of images which are opened in the Main Program Window of QuickPHOTO INDUSTRIAL 3 0 program Images which were in the time of running of the Jime Lapse Video module selected in the Thumbnail Browser will be processed Select Saved Files for Processing Up to 2000 Files select this option if the images intended for processing are saved on a hard drive After clicking the Next button a dialog box for files selection will open Select all files from which you want to create the video file Maximum number of files is affected by the length of their names and it may reach the maximum value of 2000 In case of long file names this number may be smaller Select the Whole Folder for Processing select this option if the images intended for processing are saved in a folder which does not co
158. folder where you want to save the files To continue click the Next button 21 Initial Configuration of the Application Configuration Wizard QuickPHOTO Default Folders l N DUS TRI AL In this step default folders can be defined 3 0 Default Folder for Saving Acquired Images C Program Files QuickPHOTO INDUSTRIAL 3 0 Images Default Folder for Exporting Measured Values C Program Files QuickPHOTO INDUSTRIAL 3 0 Results Default Folder for Saving Time Lapse Images C Program Files QuickPHOTO INDUSTRIAL 3 0 Sequences Default Folder for Saving Reports C Program Files QuickPHOTO INDUSTRIAL 3 0 Reports Scale Bar and Units On this page of the wizard you may select the type and size of the calibrated scale bar In the part Scale Bar Type you may set the type of the scale bar Ruler Line Segment Staple and Stripe In the part Scale Bar Size you may select one of the two automatic sizes Small Large or you may select the Custom option and type in your own desired length of the scale bar into the field Custom Size In the part Preview you will see what the scale bar will look like when using the current settings In the part Units select units in which the measured values will be displayed micrometers or millimeters To continue click the Next button 28 Initial Configuration of the Application Configuration Wizard QuickPHOTO Scale Bar and Units INDUSTRIAL In this step a scale bar type and units used for measureme
159. for Export sample 1 xls A x E sample 1 xls l x E sample 1 xls 1 jy x Part Location In this part it is possible to select a folder into which will all the files intended for export be saved Control in this part is similar as for the standard Windows dialog for saving of files J Results Ola ler zs J D a EP samplel xls Desktop E sample4 xls pe E sample5 xls T Network Part File Name and Type In this part it is possible to choose the names and types of files intended for export If you have selected more than one file in the Files for Export list and you will enter a new file 146 Main Program Window name into the File Name field the name of all selected files will be changed at the same time and the Numbering function which will ensure resolution of individual files will also be activated Changes will immediately be visible in the Files for Export list If you want to restore the original file names of selected images click the Restore button Save as Type drop down menu enables to select the type format of files being exported There are two options available Microsoft Excel Document xls and Text file txt Part Additional File Name Tags In the Additional File Name Tags part there are two buttons Button Date adds the current date to the names of files selected in the list of Files for Export Button Time adds the current time to the names of files se
160. g device set Calibration is necessary for all available magnifications of the microscope which you plan to use for your measuring What is meant by magnification of the microscope in this program is a combination of the objective s magnification and the zoom s or the magnification changer s magnification If you are using an upright or inverted microscope without the magnification changer the number of magnifications for calibration is given by the number of used objectives value of the zoom equals 1 for all objectives If you are using an upright or inverted microscope with the magnification changer the number of magnifications for calibration equals the number of objectives x the number of magnifications of the magnification changer When using a stereomicroscope with a zoom the number of magnifications for calibration equals the number of objectives x the number of magnifications click stop positions of the zooming unit Important You will need a calibration scale a stage micrometer for the calibration 33 Calibration of the Program for Measurements 2 Calibration Manager Note What is meant by calibration in this program is the process of calibration as well as the set of calibration values for individual magnifications of the microscope imaging device sets marked by Calibration Name If you want to use various types of imaging devices or microscopes for your measurements it is necessary to calibrate create a calibrat
161. g reports in the Microsoft Word Option Create Report will export images selected in the Thumbnail Browser and measured values into a new report Option Template Manager opens the Report Template Manager dialog box which enables creating of new report templates and management of the already existing templates for more information see Reports on page 148 Window Help Create Report Template Manager Menu Window Option Restore Default Program Layout is used for setting of the original layout of all parts of the Main Program Window Menu Help Menu Help contains the following items Help opens an electronic user manual You can also use the F1 key Option What Is New displays a file containing a list of all new functions of the current program version Option Send Dongle Number is used for displaying and sending off of the dongle number via e mail to obtain new modules or 70 Main Program Window program upgrades Option Licence opens a document with the description of licence conditions Option About displays information about QuickPHOTO INDUSTRIAL 3 0 program TE Help Help Restore Default Program Layout Help Fi What Is New Send Dongle Number License About 1 2 Toolbar Individual program functions can also be activated using the following icons from the Toolbar cz Open displays a dialog box for opening of images from the available disc drives for
162. ge will restore the original form of the image in which it was opened or acquired all inserted objects will be deleted Option Delete Selected Objects will delete all selected objects from the current image Option Delete All Objects will delete all inserted objects from the current image Option Merge Objects with an Image Flatten Layers will merge layers containing inserted objects with the image After merging it will no longer be possible to edit the inserted objects they will become a fixed part of the image 65 Main Program Window Option Configuration Wizard will open a guide for basic program setup For detailed description of work with Configuration Wizard see Configuration Wizard on page 25 Option Options will open a dialog box for setting of program parameters For detailed description of the setting options see Options on page 164 Edit Image View Measurements Annotations Imaging Devices Modul Undo Ctrl Z Copy Ctrl C i Select Region e Object Line Color Object Line Thickness gt Compose New Image from Layout of Displayed Images Ctrl4 L Restore Original Image X Delete Selected Objects Del Delete All Objects Merge Objects with an Image Flatten Layers Ctri Configuration Wizard Options Fil Menu Image In the menu mage it is possible to display Image Information There are also available the following options for image editing crop rotation flip mir
163. gnifications Calibration CCD Camera Upright Microscope x Zoom Magnification Changer Magnification Recalibrate Selected Magnification Remove All Magnifications Graph of Calibration Values T 3 Gej gt c 2 8 2 a g Objective Magnification OK 51 Calibration of the Program for Measurements 3 3 Creating a New Calibration Using the Magnification Encoder for Stereomicroscopes To make measurements in the QuickPHOTO INDUSTRIAL 3 0 program more comfortable it is possible to use stereomicroscopes with the so called magnification encoder It is a device connectible to the computer which detects the current zooming unit s magnification and also the position of the encoded nosepiece with objectives When acquiring an image corresponding magnification is automatically assigned to the newly acquired image Therefore it 1s not necessary to assign the microscope s magnification used for obtaining of every image manually before measuring Before you create a new calibration put the stage micrometer under the microscope set the lowest magnification and focus the microscope The process of creating a new calibration using the magnification encoder consists of three main steps e setting of the magnification encoder e adding of a new calibration with the magnification encoder for stereomicroscopes e calibration of individual magnifications using the magnification encoder for stereomicroscopes Setting th
164. gnifications of objectives in use to individual nosepiece positions Continue as follows by turning of the nosepiece choose gradually all available positions and assign them from the drop down menus with the corresponding magnification of the objectives in use Mark empty positions with Continue by clicking the Next button Calibration of the Program for Measurements Add Calibration CCD Camera Upright Microscope x Assignments of Objective Magnifications to Nosepiece Positions Objective Assignments Select each position by rotating the nosepiece and assign corresponding magnifications from the drop down boxes 1 m For empty positions keep assigned y Ja os 4 e Dialog box named Table of Magnifications Calibration Calibration Name will open and in the Zable of Calibration Values part magnifications of the objectives in use and their corresponding positions of the magnification encoder will be filled in In this dialog box will now also be active the Change Objective Positions button which opens the Assignments of Objective Magnifications to Nosepiece Positions dialog box which enables you to make changes in the assignment of the objectives to the positions of the magnification encoder 49 Calibration of the Program for Measurements Table of Magnifications Calibration CCD Camera Upright Microscope x Table of Calibration Values mm Calibrate New Magnification
165. gnment Mode Continue by clicking the Next button Add Calibration CCD Camera Upright Microscope x Magnification Encoder Selection Select magnification encoder you want to use for calibration CCD Camera Upright Microscope Only encoders configured in Edit gt Options gt Magnification Encoders menu are available Select Magnification Encoder No Magnification Encoder Manual Magnification Assignment Mode 4 Next page of the guide Zoom Magnification Changer Selection asks about the microscope equipment zooming unit or the magnification changer If the microscope in use is equipped with a zoom or a magnification changer check the corresponding option Zooming Unit or Magnification Changer Unit If the microscope is not equipped with either of these elements check the No Zoom Magnification Changer option In this case the value of zoom magnification changer in this calibration will automatically be set for all objectives on the value 1 Continue by clicking the Next button A dialog box Table of Magnifications Calibration Calibration Name will open This step will finish adding of a new calibration and you may continue with the actual calibration of particular magnifications 37 Calibration of the Program for Measurements Add Calibration CCD Camera Upright Microscope x Zoom Magnification Changer Selection Select whether your microscope is equipped with zooming or magnification c
166. h will be used as reference for the white balance The Default WB button is designed for renewal of the default white balance Manual setting of the white balance can be done using the White Balance sliders on the Colors tab Default WB Focus Region this function makes it easier to focus the selected area of ji Custom the image To use this function proceed as follows E gt Reset 1 Using the focus knob roughly focus the microscope Click the Custom button in the Focus Region part Move the cursor into the live view and insert the frame in the live view by dragging the mouse while holding the left mouse button to a place where you want to do the focusing 193 Control Panels of Imaging Devices 2 Focus indicator will be displayed next to the frame Blue strip represents the current focus value and the red guideline maximum reached focus value Both values are at the same level at the beginning Both values are also displayed in a numerical form in the title of the frame in the current value maximal reached value form 3 Ifyou start rotating the microscope focus knob now the blue strip will start to decline respectively rise according to the fact whether the focus level in the framed area will decrease or rise Select such a direction so the strip starts to rise Continue rotating the focus knob until the blue strip starts to decline Red guideline will remain on the position of the maximum reached focus value G
167. hanger unit Zoom Magnification Changer Selection o No Zoom Magnification Changer Default selection for upright and inverted microscopes without magnification changer zoom 1 Zooming Unit Default selection for stereomicroscopes Magnification Changer Unit Selection for upright and inverted microscopes with magnification changer unit Standard Calibration of Individual Magnifications In the next step calibration of individual magnifications will be done Proceed according to the following instructions l 38 In the open dialog box Table of Magnifications Calibration Calibration Name the actual process of calibration will be done In the Table of Calibration Values part you will find columns named Objective Position and Zoom Magnification Changer Magnification If you have chosen the No Zoom Magnification Changer option in step 4 while adding a calibration there will automatically be given the value 1 in the last column If you have chosen in step 4 one of the options Zooming Unit or Magnification Changer Unit this column will be empty and individual values of the zoom magnification changer will be gradually added to the table during the process of calibration of the particular magnifications Column Position is not used in this case In the right part of the dialog box there is only one active button in this moment Calibrate New Magnification There is a Graph of Calibration Values in the bottom
168. he umination part select the Reflected option Then in the Calibration mask part click the Capture mask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in focus the microscope and set the desired exposure time 7 In the Correction level part click the Preview in live image button According to the need you may using the Correction level slider adjust the correction nae ad All acquired images will now be automatically adjusted using the Shading correction function until the function is disabled or the control panel is terminated Timestamp Tab It is possible to automatically insert into the acquired images date and time of their acquisition the so called timestamp The timestamp will after acquisition of the image become a solid part of the image and it is not possible to remove it from the image other way than by cropping it EA Timestamp ee insert Orientation Preview Position top left O Disabled Date Enabled V Time 42 10 2012 17 07 22 12 10 2012 17 07 22 0 2012 1 T 0 7 22 A Timestamp Timestamp this option turns the Timestamp function on off Disabled Enabled Settings 1f you click this button a dialog box for setting of timestamp parameters will open Insert Insert by checking of individual options it is possible to define which ames components of the timestamp will be inserted into the acquired images INTE Seconds 264
169. he color selected in the Background Color part 162 Main Program Window After setting of all desired parameters of the new image click the OK button for creation of the new image Composed image will be inserted as a new image In the composed image most of the functions for measuring will not be available Image composed from two images displayed next to each other can look for example like this one 6 3 Slideshow Option View Start Slideshow runs a full screen presentation of the opened images If only one thumbnail is selected in the Thumbnail Browser all opened images will gradually be displayed If you select more images in the Thumbnail Browser the presentation will contain only these selected images Display of images in the presentation will be gradual in a regular time interval During the presentation you may browse through individual images using the keys Page Up previous image and Page Down next image While you browse through images gradual projection of images will be suspended To continue the presentation press the Space key Parameters of the presentation can be adjusted in the Options of the program Edit gt Options General Miscellaneous For the presentation you can set the delay between images and the way of its termination 163 Main Program Window 6 4 Display in Full Screen Mode Option View Display in Full Screen Mode displays the content of the Display Wi
170. he focused area From these focused areas is composed the resulting completely focused image 293 Modules Standard image Image from Deep Focus 4 RECORD IT optional RECORD IT module is an additional module which enables in the real time record digital video from digital and analog cameras and other devices Using the RECORD IT module you may easily record for example a microscopic experiment growth of cell colonies and so on 5 FLUO optional FLUO is designed for composing of images acquired using a fluorescence microscope Up to eight images acquired using various fluorescence filters can be composed into one resulting image 294 Adding of Modules and Upgrading QuickPHOTO INDUSTRIAL 3 0 program can be extended with additional modules for example Deep Focus RECORD IT FLUO or upgraded to higher version if available The first step towards getting a new module or upgrade for your QuickPHOTO INDUSTRIAL 3 0 program is sending of the dongle identification number via e mail or its communication to your supplier by another way In order to send the dongle number via e mail proceed as follows 1 In the Help menu activate the Send Dongle Number option 2 Inthe open dialog box there will be displayed the identification number of your dongle and a support promicra com e mail address If you are using one of the e mail programs for example Microsoft Outlook click the Send button New e mail will auto
171. hether you want to search for the driver on the internet Select No not this time Continue by clicking the Next button On the next tab leave marked the nstall the software automatically recommended option and click the Next button System will find appropriate device drivers and will ask you if you want to install them Click the Continue button Camera device drivers will be installed This process must be repeated one more time for installation of the second driver First time there will be installed the INFINITY Unconfigured device and for the second time a device named INFINITY X X USB 2 0 Camera 4 2 Completing the Installation when Using the IDS uEye Digital Cameras 1 If you have restarted your computer sign in again to the administrator s account 2 Connect the IDS uEye camera to the USB 2 0 port of your computer using the enclosed USB cable 3 Continue with device drivers installation as follows e Microsoft Windows 7 8 Installation and commissioning of the camera device drivers will take place automatically e Microsoft Windows Vista Found New Hardware window will appear Select Locate and install driver software recommended option User Account Control window will appear Select Continue option Windows Security window will appear Select Install this driver software anyway option Camera device drivers will be installed 19 Installation Microsoft Windows XP Found New Hardware w
172. hose control units whose support was chosen during program installation will be displayed in the list If your control unit is not available in the list exit the QuickPHOTO INDUSTRIAL 3 0 program and run the program installation from the installation CD again On page Installation Type Selection select the Update Existing Installation Add Components option and on page Select Additional Components Magnification Encoders choose from the list a control unit whose support you want to add Options 4 General Default Folders Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators x If using the magnification encoder activate it by checking the respective checkbox and select type of the control unit Upright Inverted Microscopes V Use Objective Encoder Control Unit PRO NPENC Test Device Stereomicroscopes V Use Zoom Objective Encoder Control Unit SZX2 ZB16 ZE SZX2 ZB10 ZE Test Device Objective Encoder Device SZX2 2RE16 E SZX2 2RE10 Backup settings g Load Settings Configuration Wizard OK E Tab Illuminators This tab is intended for setti
173. humbnail of the mask will be displayed in the control panel 250 Control Panels of Imaging Devices 4 Place the specimen back in 5 In the Correction level part adjust the correction level using the slider Acquire an image to check the settings According to the need you may using the Correction level slider adjust the correction Reflected Light 1 Set the desired microscope s magnification 2 Place a white sheet under the microscope for example a sheet of white paper Do not focus the microscope On the Capture tab set the desired image Quality Resolution and White balance 3 Switch to the Shading correction tab and select Enabled option In the umination part select the Reflected option Then click the Capture mask button A thumbnail of the mask will be displayed in the control panel 4 Insert the specimen under the microscope and focus it 5 In the Correction level part adjust the correction level using the slider Acquire an image to check the settings According to the need you may using the Correction level slider adjust the correction All acquired images will now be automatically adjusted using the Shading correction function until the function is disabled or the control panel is terminated Timestamp Tab It is possible to automatically insert into the acquired images date and time of their acquisition the so called timestamp The timestamp will after acquisition of the image become a solid par
174. hutter speed and Aperture these options enable manual settings of exposure parameters if Manual Shutter priority or Aperture priority modes are selected in Metering system menu Shutter speed 17125 Aperture Live Image Tab This tab contains the controls intended for setting the live view The available parameters are Brightness Contrast Saturation Hue Borders These settings do not influence the captured images Performed changes will apply to the live view only Using the sliders you can set the properties of the live view The recommended procedure is 1 Open the control panel of the OLYMPUS SP and CAMEDIA series digital cameras capture the image and display it in the Display Window 2 In the control panel of the OLYMPUS SP and CAMEDIA series digital cameras activate the Live image tab Move the control panel to see the live view and the captured image at once 3 Using the sliders set the live view parameters so that it looks as similar as possible to the captured image Borders slider can be used for setting the optimal size of the live view Move the slider so that the live view fills the whole control panel s window and black borders are eliminated Click the Default button to restore the default settings of all the control elements on the Live image tab 271 Control Panels of Imaging Devices Live image Brightness Contrast Saturation Hue Borders L O Profiles Profiles are intended for q
175. iads 58 Mam Program WiNdoW anname kanor ee en E A TRA re 61 1 Description of the Graphical User Interface 2 0 0 0 ccc cece ec ec cence e ene eeeenens 62 We To VISTA TSAR of cie es ceestaat re tres auth hate i oatalaae bh Saat ta deer antnecaer syste matee is caneroganree 64 12 ROOM Al aa a E 71 1 3 una Di atl FO W SCL sei T T T T eindaute nines saseaeeas 76 Das INAV Oe Seah eee cel Cl cee Te Ml ter alent Gl chants 78 K3 DIS Play WIN OW scsi ces oes et ee eee eee 79 t6 Table OF Measured Va MeS it ccehucubastalabt noah a nee htontouns 79 De UAVS BA moneen aE a a S 80 2 ACUN OL ING W J OCS ese e bs a e AE 80 2 Selection or the Imasing Devio stokes 3st datstetec ee ahh eihad acuta ns adededen ees 80 2 2 Opening of the Imaging Device s Control Panel cceceeecec eee ee ee es 81 DZ OPC QUE MIO AN IA OSs ia a crete clin ee TORA ap em weer a en ee 82 Be WV OER WEI LDA OSS ap Nah lat ot eae chart lire Sorta bates ahr deat ea 82 Jk Tma e ole olin Sauer Peete er moore mee eer omens tne nent Tarte ran cee eee eee ne Oane Feeeee rere 82 72s Image EUNE 25 5 hot andes eae Mother hued noe deanna coed eo waren tacmatal 83 3 3 Indication and Annotation of Interesting Areas cc cece ec eceeeee ee ee sees 90 Bi IMAG SAV NINO ere ck tGi crash ek aac eg ate Loe iain ee eal ia cde Nae e seh a 97 Shee Aan Da ay Ske ld 0 1 Oko ee RES eee Te Reece ROSEY OY RETRO PeRTE SO eT oe 108 A Measurements 26 sacchs nid aes tet eacten hana a
176. ifications Adding a New Standard Calibration To add a new calibration use the following procedure 1 Open the Calibration Manager dialog box by activating the Measurements gt Calibration Manager Ctrl Alt C option 35 Calibration of the Program for Measurements 2 After clicking the Add Calibration button a guide Add Calibration opens where in 36 the first step you choose the name of the calibration being created The name of a calibration consists of two parts from the name of the imaging device and from the microscope type At first choose the type of the used imaging device from the drop down list in the maging Device part or click Custom option and enter your own name of the imaging device In the Microscope part enter the type of the used microscope Continue by clicking the Next button Calibration Manager x Calibration Name Objective Encoder Zoom Encoder Add Calibration B ioc s a ae f SST A AA Add Calibration x Calibration Name Select imaging device and microscope type to define calibration name Imaging Device Select from the List Custom max 30 chars CCD Camera Microscope Type max 30 chars Upright Microscope Next gt Cancel Calibration of the Program for Measurements 3 Next page of the guide Magnification Encoder Selection will have only one active option No Magnification Encoder Manual Magnification Assi
177. igure your device in the program Options Edit gt Options Hardware OLYMPUS SLR Consumer Cameras or by the Configuration Wizard of the program Edit Configuration Wizard 308 Troubleshooting FAQ Live View from Analog Video Sources There is no live view displayed in the control panel of the analog video source l 2 Make sure the video output of the analog video signal source is connected to the device for live view displaying Make sure in the QuickPHOTO INDUSTRIAL 3 0 program in the Options Edit gt Options Hardware Analog Video Source there is set the Video Standard option corresponding with the analog video source used video standard and the selected Input option corresponds with the live view displaying device used input connector To restore the default settings of live view you may on this tab use the Restore button Make sure in your operating system is not installed any of the codec packages for example K Lite Codec Pack Codec Pack Allin 1 ACE Mega CoDecS Pack Vobsub ffdshow and so on If you have some of the packages installed in your computer uninstall it Live view from the analog video source on the computer monitor has a low quality it is checkered or snatchy If you are using for displaying of live view on your computer monitor an external USB device proceed as follows l Make sure the USB device is connected to the USB 2 0 port If your computer do
178. indow and the iam Undock button will change to the Dock button Clicking this button will return the table to the original position Buttons in the right hand part of the toolbar act as filters and they display in the Zable of Measured Values from all measured values only the corresponding measurands Other measurands contained in the table will be hidden EE All button will display in the Zable of Measured Values all values measured in the image The only exception are hardness values which are displayed only in a stand alone AJ mode after clicking the Hardness iness button 141 Main Program Window x Dimensions meses button will leave displayed in the Zable of Measured Values only values of measured dimensions Perimeters s button will leave displayed in the Table of Measured Values only values of the measured perimeters Areas s button will leave displayed in the Zable of Measured Values only values of the measured areas A Angles s button will leave displayed in the Table of Measured Values only values of the measured angles Objects ove button will leave displayed in the Table of Measured Values only the object counts 7 Phases s button will leave displayed in the Table of Measured Values only values of measured phases X ae l Hardness s button will display in the Table of Measured Values the measured hardness values E Table of Measured Values sample5 jpg Measur
179. indow will appear You will be asked whether you want to search for the driver on the internet Select No not this time Continue by clicking the Next button On the next tab leave marked the Install the software automatically recommended option and click the Next button System will find appropriate device drivers and will ask you if you want to install them Click the Continue button Camera device drivers will be installed This process must be repeated one more time for installation of the second driver First time there will be installed the wEye boot device and for the second time a device named uEye UI xxxx Series When you finish the above described process a green LED should light up on the camera if the camera 1s equipped with this LED This LED signals that the camera has been properly installed and is ready to use If the LED light remains red the camera probably is not connected to the USB 2 0 port or there has been a problem with the device drivers installation 4 3 Completing the Installation when Using the PathScan Enabler IV Digital Microscope 1 If you have restarted your computer sign in again to the administrator s account 2 Connect the PathScan Enabler IV digital microscope to the USB 2 0 port of your computer using the enclosed USB cable and turn it on 3 Continue with device drivers installation as follows e Microsoft Windows Vista 7 8 Installation and commissioning of the device drivers will take pla
180. ing also from imaging devices controlled via TWAIN interface These control panels are controlled externally by the Timer module which implies minor limitations After you begin the time lapse image capturing by clicking the Start button using the TWAIN control panel an informative dialog box will be displayed which will ask you to click the button for image capturing in the control panel of the imaging device After you click the corresponding button initialization image will be captured which will not be included in the time lapse sequence which will enable the Timer module to identify the position of the button for image capturing in the TWAIN control panel Information dialog box will automatically disappear after capturing of this image and the time lapse image capturing will begin To ensure successful process of time lapse image capturing do not move or click the mouse anymore until the whole time lapse image capturing process 1s finished 2 Time Lapse Video Built in Module Time Lapse Video module which is a standard component of the QuickPHOTO INDUSTRIAL 3 0 program is designed for creating of video files from the acquired time lapse images For example if you acquire a series of images of a slowly changing sample with the Timer module you can then easily create using the Time Lapse Video module a video file in which the movement will be displayed in an accelerated form How to Create a Time Lapse Video To create a video
181. ing part Button Time will add the current time to the image names selected in the Thumbnails of Images for Saving part You can combine the use of both of these buttons Part Numbering Part Numbering contains the Numbering button which adds a serial number to the names of all images in Thumbnails of Images for Saving part Images will always be numbered from the number 00001 The use of the Numbering button can be freely combined with the use of Date and Time buttons This function is useful for example in case you want to save several images from the same series with the same name and you want to distinguish them with a serial number Part Flattening Layers In this part of the window there is the Flattening Layers option available for more information see Information about Work with Layers on page 97 Setting of this option will affect all images to be saved The first option Keep Inserted Objects in a Separate Layer 1s valid only for JPEG and TIFF type images With the BMP and PNG types layers will always be merged automatically Part Information In the Information part explanatory notes are displayed for indicators and warnings about important facts about saving of images Information The red labeled images have the same name and type It is necessary to rename some images before saving Images with yellow exclamation mark already exist in the current folder and will be overwritten Some images are going to b
182. inserts a calibrated vertical scale bar into the right upper corner of the image Measure Line Segment activates a tool for measuring of the length of a line segment i Measure Ellipse activates a tool for measuring of the perimeter and area of an ellipse 1 Measure Rectangle activates a tool for measuring of the perimeter and area of a rectangle 10 amp Measure Circle click on the arrow offering a drop down menu and choose one of the options Circle Defined by Radius Circle Defined by Diameter or Circle Defined by 3 Points After you choose one ofthe options a tool for measuring of the diameter perimeter and the area of a circle will be activated 12 4 Main Program Window Measure Polygon Polyline click on the arrow offering a drop down menu and choose one of the options Polygon or Polyline After you choose one of the options a tool for measuring of the polygon s perimeter and area or for measuring of the polyline s length will be activated Count Objects after clicking on the arrow offering the drop down menu you may choose one of the options Count Objects New Group to initiate counting of the new group of objects or Add Objects to the Current Group for adding of further objects to an already existing group Measure Angle after clicking on the arrow offering the drop down menu choose one of the options Arbitrary Angle Angle from the X Axis Angle from the Y Axis or Angle Defined by 2 Line Segment
183. ion for each used set of microscope imaging device It is possible to switch between the created calibrations according to the actual set in use There is a program function named Calibration Manager for creating of and subsequent administration of calibrations which can be found in the menu Measurements Calibration Manager Ctrl Alt C Calibration Manager is used for creating and administrating of all calibrations created for individual microscope imaging device measuring sets Calibration Manager x Calibration Name Objective Encoder Zoom Encoder Add Calibration CCD Camera Upright Microscope g a aca i a n RE l a a Ee So i men Remove Calibration Set as Active Table of Magnifications Last Calibrations Modification 24 8 2012 15 03 46 5 Lock Calibrations fey Backup Calibrations a Load Calibrations Close To create a new calibration click the Add Calibration button in the Calibration Manager dialog box To delete a chosen calibration from the list click the Remove Calibration button To change the name of a created calibration click the Rename Calibration button If there is more than one listed calibration calibration chosen for measuring can be activated by clicking the Set as Active button for more information see Setting an Active Calibration on page 58 34 Calibration of the Program for Measurements After a calibration is marked by
184. is and this way defined angle will be calculated Control Panels of Imaging Devices A Measure Angle from the Y Axis after you click this button function for measuring of an angle from the Y axis will be activated By clicking and dragging the left mouse button or by clicking the left mouse button into the beginning and the end point insert the first arm of an angle This arm will automatically be completed with the vertical axis and this way defined angle will be calculated Measure Angle Defined by 2 Line Segments after you click this button function for measuring ofan angle defined by two line segments will be activated By clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and the end point insert the line segment defining the first arm of an angle Then in a similar way insert line segment for the second arm of an angle The arms of an angle will be created from both of the line segments by elongation using auxiliary lines If you want to create a precisely horizontal or vertical arm of an angle hold the Shift key while creating it The inserted line segments should not cross each other ze In this part it is possible to select units in which measured amp pm m values will be displayed exported If you want to measure the convex or acute angle check in Convex Acute Angle the Measured Angle part the option Convex Acute Angle If be ttm you want to me
185. isconnect the camera If you turn off the camera while running the control panel or if you disconnect the USB cable it may cause the above mentioned getting stuck of the camera In this case disconnect the USB cable and the mains if it is connected and take the battery out of the camera Wait a few seconds insert the battery back in and turn the camera back on After all these steps the camera should work correctly again Camera is not available as a Mass Storage device Is this a standard situation 303 Troubleshooting FAQ Yes if you switch the camera to the PC CONTROL mode it is not possible to access it as a Mass Storage device If you want to work with the camera like with a removable disc it is necessary to turn off the PC CONTROL mode for more information see the section called Installation of OLYMPUS Digital Camera on page 14 The necessity to switch the mode of the camera will drop out if you use the USB card reader The possibilities to control the OLYMPUS digital cameras from the QuickPHOTO INDUSTRIAL 3 0 program from the standard user accounts without administrator s privileges in the Microsoft Windows XP Vista 7 8 operating systems e OLYMPUS E 330 Digital SLR Camera This camera cannot be used in the Microsoft Windows Vista 7 8 operating systems at all To use this camera in the Microsoft Windows XP operating system it is necessary to use an account with the administrator s privileg
186. isplay central cross Settings Tab This tab contains control elements which are intended for adjustment of the parameters of the live view Changes in the settings will apply to the live view as well as to the acquired images a Settings Live image quality Image settings Manual white balance 1280 x 960 Brightness J 0 Sharpness g 0 Rea J C Bayer mode Contrast g 0 Gain g 30 Blue J 100 OMmodet OMode3 Omode2 Mode 4 ne J 5 piai y 1 00 Saturation J 0 eae Live image quality here select the resolution of the live view When 1260 x 960 e e the highest resolution is set the Auto exposure mode will not be available Bayer mode Bayer mode each of these options differently balances the RGB O Modei Mode 3 Modez Mode 4 color levels Brightness the slider sets camera s brightness 235 Control Panels of Imaging Devices Contrast the slider sets camera s contrast Hue the slider sets camera s hue Sharpness the slider sets camera s sharpness Gain the slider affects the signal s strength coming from the camera With higher gain values noise in the image may be more visible Gamma the slider is used to set gamma correction combination of the brightness and contrast adjustment Image settings Brightness J 0 Sharpness J 0 Contrast J 0 Gain J 30 Hue 9 o Gamma J 1 00 Manual white balance Manual white balance sliders enable to manually adjust Red the levels of the red and the blue channel Blue J 100
187. ith the avi extension will be displayed Select the desired location and enter the file name By clicking the Save button you will run the video file creation itself You may watch the process of creating of the video file on the Video Rendering page Using the Rendering Priority option it is possible to select how busy will the computer be with creating of the video file If you want to work with another programs during creation of the video file you may select the Low option You may pause creating of the video file by clicking the Pause button and you may run it again by clicking the Resume button Modules YB Time Lapse Video Video Rendering Information about the rendering process is displayed on this page Total Number of Images Current Image 63 Elapsed Time 00 00 06 8 Remaining Time 00 00 11 a V Display Current Image Rendering Priority 3 Deep Focus optional Deep Focus module is designed for creating of images with much higher depth of field than the standard use of optical microscopes can provide so called focus stacking or z stacking This module can be used with stereomicroscopes as well as other types of optical microscopes for observation in transmitted or reflected light This module can also be used for macro imaging Principle is in the capturing of a sequence of images with a different focus plane From each of these images is by the module automatically selected and used only t
188. itializes the indicator again into the default state Minimum sharpness sets the minimum level of sharpness bl Standard sharpness sets the normal level of sharpness gt Maximum sharpness sets the maximum level of sharpness Low contrast reduces the level of image contrast Standard contrast sets the standard contrast value High contrast increases the level of image contrast e ej e 23 Control Panels of Imaging Devices amp Linear contrast sets the contrast to linear value ae White balance on defined region after clicking this icon you can define by dragging the mouse in the live view while holding the left mouse button the area for white balance Black balance on defined region after clicking this icon you can define by dragging the mouse in the live view while holding the left mouse button the area for black balance Automatic white balance by clicking this button the white balance will be set automatically Automatic black balance by clicking this button the black balance will be set automatically ae Show hide automatic exposure region indicator shows or hides the exposure I region Capture Tab This tab contains camera controls for setting the exposure parameters and acquiring the images These settings apply to the live view as well as to the acquired images Fy Capture p Image Binning Accumulation multiple images l Size 4140x3096 v
189. ivates the function for automatic white balance It is Auto WB best to do this balancing when the camera is capturing a white area ie ICTs Live View Quality here select the desired resolution of the live view 1260 x 960 w ae islets Exposure Mode if the exposure mode is set to the Auto option exposure Aut AEL o time is automatically set according to the illumination of the camera s field of view When using the Auto mode the AEL button Auto Exposure Lock will also be active This button locks the exposure time on the current value If the Manual option is active value of the exposure time is set by user using the control elements in the Exposure Time part posita Padci Exposure Region this control element is designed for defining of an 100 area of the live view which will be used for measuring of exposition 30 in the Auto exposure mode You may select one of the predefined f re exposure region sizes 100 the whole area of the live view 30 me and 7 or you may select your own size of the region If you select the 30 or 1 option a frame will be displayed in the middle of the live view which will define the exposure region The frame can be moved to the desired area in the image move the mouse into the region and by dragging the mouse while holding the left mouse button relocate the frame to the desired position If you place the cursor above the frame s edge or above one of its corners the curso
190. jects will be canceled Note Select tool is automatically activated every time no other tool is active Change of Line Color and Thickness of the Drawn Objects In order to edit inserted objects select them first for more information see Selection of the Inserted Objects for Editing on page 95 The color and line thickness of selected objects may be changed For these purposes use the following options Edit Object Line Color Edit gt Object Line Thickness or the corresponding icon 3 7 in the Toolbar New line color of the selected objects can be chosen from the displayed palette after the option Edit Object Line Color was activated or after clicking the left part of the 5 icon 95 Main Program Window New line thickness of the selected objects can be chosen from the displayed list after the option Edit Object Line Thickness was activated or after clicking on the right part of the TE icon Relocation Change of Size and Shape of the Drawn Objects Relocation of Text Annotations In order to edit inserted objects select them first for more information see Selection of the Inserted Objects for Editing on page 95 If you want to move the selected object in the image move the mouse cursor to its contour line Cursor will change to P By clicking on the object s outline and by dragging the mouse while holding the left mouse button the object can be moved to the desired area In th
191. justment of the control panel s elements will take place 267 Control Panels of Imaging Devices 8 OLYMPUS Consumer Digital Cameras SP and CAMEDIA series i OLYMPUS digital camera control panel C 7070WIDEZOOM Se A Em Le Profiles a A 4 G Connect Live image Refresh Erase Settings Standard Microscope Macro Custom Camera control amp Live image Quality Sensitivity White balance SHO v auo Auto Capture re Max Flash mode Focus mode Metering system Exposure compensation Auto v Auto v Digital ESP v o g E Shutter speed 1 125 Aperture SSS 5 6 Full screen Fin Gouo Guan aon oa GUST OOO U DOOU si The control panel is intended for displaying a live image controlling the camera functions and capturing images from OLYMPUS SP and CAMEDIA series digital cameras The controls are located in the toolbar and two tabs Camera Control Tab The tab contains the controls for setting the camera parameters setting the exposure and capturing images 268 Control Panels of Imaging Devices Camera control Quality Sensitivity White balance Zoom SHa v Auto Auto a 9 Capture i Min Max Flash mode Focus mode Metering system Exposure compensation Auto v Auto v Digital ESP v lo v Full screen a Capture after clicking this button an image will be acquired and Capture automatically transferred to the computer You
192. l inserted objects There are two possible ways to save the images with layers kept as they are or with flattened merged layers The saving option named Flattening Layers is available in the Saving Parameters and Save As windows and contains the following options 1 Keep Inserted Objects in a Separate Layer 2 Merge Objects with an Image Flatten Layers Both saving options have their specifics 1 Keep Inserted Objects in a Separate Layer It is possible to keep the layers separate only when saving images as the JPEG or TIFF type Images of the BMP and PNG type do not support saving of layers If you save the image as a JPEG or TIFF type with the layers kept all its functions will be available next time you open it in the QuickPHOTO INDUSTRIAL 3 0 program Which means that the image will have an assigned calibration which was used for measurements it will be possible to edit all inserted objects description of the image will be maintained the table of measured values will contain measurement results with possibly added comments Image saved in such a way will in case a calibration was assigned to this image before saving contain complete information about the calibration and it will be possible to continue with measurements in the image also at a different workstation equipped with the QuickPHOTO INDUSTRIAL 3 0 program even if this workstation does not have the calibration which was used for acquiring of the image available for the used
193. l remember the last number of the acquired image when being terminated and when you run the program again numbering of the acquired images will continue When automatic image saving is set only the Build on Previous Numbering option will be available At the same time the How to Solve Name Conflicts Automatic Saving part will be made available This option defines the program behavior in cases when a conflict appears during automatic image saving in the names of the newly saved and previously saved images There are two options available Increase the Image Number Automatically newly saved image will be labeled with a one step higher number than the number of the image with the highest number in the folder Further on the program will for newly acquired images pick up where this numbering has ended Overwrite Existing Images option will cause automatic overwriting of already saved images by the newly acquired ones without displaying any warning For automatic saving of new images is also available a Flattering Layers during Automatic Image Saving part If you activate the Keep Layers option acquired images being saved as JPEG or TIFF type with be saved with layers kept This option is suitable for example for applications when a microscope is equipped with a magnification encoder and on the 167 Main Program Window Measurements Scale Bar tab the Automatic Scale Bar Insertion function 1s active If the Automatically Flatten Layers
194. layers kept as they are PNG type is especially suitable for publishing of smaller images on the internet e Information about BMP File Type BMP file type Windows Bitmap suffix bmp represents in terms of how to save image data the most primitive way of saving images to a file BMP type saves image information to a file without compression because of that the result is a very large file BMP type does not allow in the QuickPHOTO INDUSTRIAL 3 0 program saving of images with the layers kept as they are Note Program QuickPHOTO INDUSTRIAL 3 0 saves images to files in the 24bit RGB mode and saved images have the DPI parameter set at the value 300 Image Saving There are several options for saving images in the File menu These are the following options Save Save As Save All Save All As You can also use key shortcuts to activate individual options 100 Main Program Window Save Ctrl S Save As Shift Ctrl S Save All Ctri Alt S Save All As Shift Ctri Alt S Option Save Option File Save or the icon in the Toolbar will save only those images selected in the Thumbnail Browser If the images being saved have already been saved before they will be saved again into their original locations If the JPEG or TIFF types of images are being re saved into their original locations a Saving Parameters dialog box will be displayed Other types of images BMP and PNG will be re saved automatically If you are sa
195. ld be connected the CINCH connector of the cable of the TV output of the camera Make sure in the QuickPHOTO INDUSTRIAL 3 0 program Options Edit Options Hardware OLYMPUS SLR Consumer Cameras the Video Standard is set to PAL or PAL B value and the nput is set to Composite To restore the default settings of the device for live view displaying you may use the Restore button on this tab Make sure the video output of the OLYMPUS camera is set on PAL Proceed as follows OLYMPUS E system Series Digital SLR Cameras Disconnect all cables from the camera Activate the camera s menu In the Settings 2 submenu symbol of a key with the number 2 on it find the VIDEO OUT option and make sure the PAL option is active OLYMPUS CAMEDIA and SP Series Consumer Digital Cameras Disconnect all cables from the camera except for the power cable Activate the camera s menu In the Set submenu find the VIDEO OUT option and make sure the PAL option is active Further on find the ALL RESET option and set it to OFF value Make sure on the Live image tab in the control panel the values of brightness contrast saturation and hue has proper values For setting of the default values use the Default button Make sure in your operating system is not installed any of the codec packages for example K Lite Codec Pack Codec Pack Allin 1 ACE Mega CoDecS Pack Vobsub ffdshow and so on If you have some of these packages installed i
196. least couple of seconds for illumination stabilization before capturing If you check the Turn off Illumination when Finished option the illumination will be turned off when the whole process of time lapse image capturing is finished In the umination Control part set the desired illumination intensity After the beginning of the time lapse image capturing the Current Illumination Intensity will be set as the Intensity Set for Capturing During the time lapse capturing it is possible to in the time when the illumination is turned off turn the illumination on for example for the control of the ongoing experiment using the control elements directly on the illuminator or using the Turn on button in the umination Control part Change of illumination settings will have no effect on the previously made settings for image capturing Time lapse image capturing will continue with the illumination intensity set in the ntensity Set for Capturing part If you want to during the time lapse image capturing change the illumination intensity set for capturing change the value in the Jntensity Set for Capturing part or in the Illumination Control part set while having the illumination turned on the desired intensity and click the Use Current button in the Intensity Set for Capturing part The new illumination intensity value will be set for time lapse image capturing 286 Modules Timer x Capturing Illumination Control during
197. lected in the list of Files for Export The use of both buttons can be combined Part Numbering Part Numbering contains the Numbering button which adds a serial number to all file names on the Files for Export list Files will always be numbered beginning with the number 00001 This function is useful for example if you want to save several files with the same name and distinguish them with a serial number The use of the Numbering button can be freely combined with the use of Date and Time buttons Part Information In the nformation part the explanatory notes for indicators are displayed Export Measured values to Microsoft s Excel Continuously QuickPHOTO INDUSTRIAL 3 0 program is equipped with a function for continuous exporting of measured values to the Microsoft Excel program If this function is active the measured values will be displayed not only in the Zable of Measured Values but they will be also immediately inserted into the open workbook of the Microsoft Excel program to the position of the cursor The cursor will then automatically move to the next cell Enter will be inserted Values will be exported without units and labels This function can be used for example for continuous automatic evaluation of measurements when the opened workbook in the Microsoft Excel contains suitable formulas Before you activate the continuous exporting the Microsoft Excel program must be running and a workbook with permi
198. lution unit Inch Software Version 1 3 tae ome Bi Date and time 0000 00 00 00 00 00 Actual Height 974 um Exposure Time 1 100 sec ee Delete Description FNumber 6 3 Description banaman ana Exposure program Creative Here the image description can be entered e 0221 Date and time original 0000 00 00 00 00 00 Date and time digitized 0000 00 00 00 00 00 E an z d OK OK 160 Main Program Window Q Image Information samplel jpg Image Metadata Calibration Calibration CCD Camera Upright Microscope Objective 10 Zoom 1 pu Change Image Calibration si 6 2 Compose New Image from Layout of Displayed Images QuickPHOTO INDUSTRIAL 3 0 program is equipped with a function which enables us to compose two to four images displayed next to each other in the Display Window into one new image Composed image will contain those parts of the images which are currently visible in the Display Window To compose a new image proceed as follows l 2 Switch the Display Window to a mode for displaying of two or four images next to Display the desired images intended for composing in the individual fields of the Display Window and set the desired display size Activate the Edit Compose New Image from Layout of Displayed Images Ctrl L Edit Compose New Image from Layout of Displayed Images option is available only when the Display Window is switched to the mode for displayi
199. ly be attached to the selected string for each new image Current date can also be attached next to the serial number when acquiring an image using the Date button or possibly current time using the Time button Preview of the set name is displayed in the Example field Default button renews the default settings of the name Images from Time Lapse Capturing part contains identical elements for setting of names of the time lapse images such as the Acquired Images part Settings will take effect for images acquired by the Timer module directly into the program 165 Main Program Window Options 4 General Default Folders Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Names of New Images Acquired Images P Date Time Default Example P00001 jpg Images from Time Lapse Capturing T _Date _Time Default Example T00001 jpg Store Images after Acquisition in Type of New Images Program Only S Type JPEG jpg Folder for Automatic Image Saving Numbering of Acquired Images How to Solve Name Conflicts Automatic Saving Other Settings 4 Hardware Always Begin Numbering from 00001 after Program Start up z ase the Image utomatica Imaging Devices
200. m and activate Options of the program Edit Options In the Hardware part activate the Magnification Encoders tab In the Upright Inverted Microscopes part check the Use Objective Encoder option and in the Control Unit drop down menu choose the control unit of the magnification encoder Correct function of the magnification encoder can be verified by clicking the Test Device button In case of a successful verification of the device s functionality close the Options window by clicking the OK button 46 Calibration of the Program for Measurements Note Only those control units whose support was chosen during program installation will appear in the list If your control unit is not available in the list exit the QuickPHOTO INDUSTRIAL 3 0 program and run the program installation from the installation CD again On page Installation Type Selection choose the Update Existing Installation Add Components option and on page Select Additional Components Magnification Encoders choose from the list a control unit whose support would you like to add Options 4 General If using the magnification encoder activate it by checking the respective checkbox and select type of the control unit Default Folders Image Names amp Saving Sound Language Upright Inverted Microscopes Do Not Show This Window 7 Use Objective Encoder Annotations Appearance and Layout Control Unit PRO NPENC Test Device
201. m website or possibly contact our technical support department using the following e mail address support promicra com S12 System Requirements Minimal System Requirements e Single core 2 4 GHz or multi core processor e 1GB RAM e CD ROM e 2x USB 2 0 3 0 port Supported Operating Systems e Microsoft Windows XP SP3 Vista 7 8 Minimal Monitor Resolution e 1024 x 768 pixels e 24 or 32bit color depth Supported Imaging Devices You can find the list of currently supported imaging devices on the www promicra com cameras php website Microsoft Windows Excel Outlook are registered trademarks of Microsoft Corp OLYMPUS CAMEDIA are registered trademarks of OLYMPUS Corp uEye is a registered trademark of IDS Imaging Development Systems Inc Lumenera is a registered trademark of Lumenera Corp isa registered trademark of Canon Corp Canon QuickPHOTO is a registered trademark of PROMICRA s r o v 1561 IIl 313 System Requirements 314 Index A Acquiring of New Images 80 Acquiring an Image 82 Opening of the Imaging Device s Control Panel 81 Selection of the Imaging Device 80 Adding of Modules and Upgrading 295 C Calibration of the Measurements 33 Calibration Manager 34 Creating a New Calibration 35 Creating a New Calibration Using the Magnification Encoder for Stereomicroscopes 52 Creating a New Calibration Using the Magnification Encoder for Upright and Inverte
202. mages next to each other the default orientation will be landscape to print two images next to each other the default orientation will be portrait Color Mode part contains two options of the print mode Color and Grey Scale Set the number of copies in the Copies part In the Units part choose units for setting of margins and the print size You can choose between cm or inches In the Margins part set the desired page margins for printing Minimal Margins button will set the minimal margin size which the currently selected printer allows Page Layout Tab Printer Pace Layout Image Size Original Size from Image DPI Fit to Page Keep Aspect Ratio Stretch to Page No Aspect Ratio ee te mae rs Image Name Date and Time of Image Position Origin Description Position Image Borders On Off 112 Main Program Window Image Size part contains the following options Original Size from Image DPI this option sets the original print size according to the image DPI QuickPHOTO INDUSTRIAL 3 0 program assigns to the acquired images the 300 DPI quality Fit to Page Keep Aspect Ratio this option uses the whole page area for printing and it keeps the original aspect ratio of the image Stretch to Page No Aspect Ratio this option uses the whole page area for printing Original aspect ratio will not be preserved Custom options in this part en
203. manually focus by the Focus slider Warning Turning the automatic focusing off is necessary to obtain the correct measurement results To achieve this select the Manual focus mode and move the Focus slider to infinity value Metering system Metering system here you can select the metering mode Digital ESP ui a and Spot modes represent two automatic modes The Manual option enables to set your own exposure parameters using the Shutter speed Shutter priority slider and Aperture drop down menu If you select the Shutter priority Aperture priori option you can set the Shutter speed by a slider and the corresponding aperture value will be set automatically If you select Aperture priority you can set the required Aperture value and the corresponding shutter speed will be set automatically Exposure compensation Exposure compensation this option allows to lighten or darken the images when the automatic exposure mode is set Zoom Zoom this slider controls the focal length of the camera s lens When using J certain types of digital cameras a stop will be indicated at the Zoom slider an This stop represents the value to which the slider should be set for 270 Control Panels of Imaging Devices acquiring images from a microscope If the stop is not indicated set the Zoom slider to its maximum value Warning It is necessary to maintain the fixed Zoom slider value in order to achieve correct measurements results S
204. matically be opened with the number of your dongle in it Fill in your contact data and the name of a product you want to purchase and send the e mail If you do not use an e mail program create an e mail the regular way for example using your internet browser copy your dongle number into it write your contact data and the name of a product you want to purchase and send it to the following address support promicra com 295 Adding of Modules and Upgrading 296 List of Key Shortcuts Key shortcuts used in the Main Window of the QuickPHOTO INDUSTRIAL 3 0 program Open images from available disc units Save images selected in the Thumbnail Browser Shift Ctrl S Save As saves images selected in the Thumbnail Browser into the selected location Ctrl Alt S Save All saves all opened images Shift Ctrl Alt S Save All As saves all opened images into the selected location 297 List of Key Shortcuts CHA Select all thumbnails in the Thumbnail Browser all thumbnails in the Thumbnail Browser a with a mouse Pech cee a a discontinuous selection of thumbnails in the Thumbnail Browser Shift selection with Selecting of more inserted object or a continuous selection a mouse of thumbnails in the Thumbnail Browser Shift during object insertion Inserting a horizontal or vertical line Page UP Display the previous image in the presentation mode Display the following image in the presentation mode Continue the slideshow
205. men back in and set the desired exposure time 7 In the Correction Level part click the Preview in Live View button According to the 228 need you may using the Correction Level slider adjust the correction Control Panels of Imaging Devices Reflected Light l Set the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Place a white sheet under the microscope for example a sheet of white paper Do not focus the microscope and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Auto WB button automatic white balance on the Image tab 4 On the Image tab set the desired image resolution 5 Switch to the Shading Correction tab and select the Enabled option In the umination Type part select the Reflected option Then in the Calibration Mask part click the Capture Mask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in focus the microscope and set the desired exposure time 7 In the Correction Level part click the Preview in Live View button According to the need you may using the Correction Level slider adjust the correction All acquired images will now be automatically adjusted using
206. mer module for some reason will not be able to capture images in the defined time interval the Time until Next Capturing label will be red In such case a text file containing information about the delay or possibly omission of the concerned images will be created 1 1 Tab Capturing Capturing tab contains control elements for the setting of parameters of the time lapse image capturing You may select from two modes 283 Modules e Number of Images e Time Interval Image Capturing in the Number of Images Mode After you select the Number of Images mode set the number of images you want to capture in the Number field In the Interval between Images part set the desired time interval in hours minutes and seconds Image Capturing in the Time Interval Mode After you select the Time Interval option set the Beginning and End of the time interval intended for time lapse image capturing Beginning and end of the interval is defined by the date and time The beginning and end date can be defined by typing in from the keyboard or by selecting it from a calendar which can be displayed by clicking the arrow on the right from the displayed date Time values can be entered from the keyboard or using the arrows on the right hand side next to the corresponding time fields Then in the Interval between Images part set the desired time interval in hours minutes and seconds Note Minimal interval between images depends on the size of th
207. mera stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification SZX 16 Recalibrate Selected Magnification Objective Position 0 7 0 8 1 125 1 6 2 2 5 Pa 0 5 e 4115427 35 7593 28 4464 22 7975 17 7778 14 2466 11 4285 8 9366 Remove Selected Magnification Selected Magnification 1 2 21 1267 18 3047 14 6645 11 7816 9 1630 7 3325 5 8361 4 5415 Remove All Magnifications Change Objective Positions Graph of Calibration Values E T 5 gt E Q 5 5 a Q _ _ _ Zoom Magnification OK If there has been a mistake in the calibration process all values in the Graph of Calibration Values will not lay on a straight line On the following image there has been an incorrect calibration of the zoom magnification 2 5 This particular value must be recalibrated Graph of Calibration Values 1 Calibration Value 1 mm _ _ _ Zoom Magnification H C Table of Magnifications Calibration CCD camera stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Objective Position 0 7 0 8 1 1 25 1 6 2 A 1 17 1308 14 9644 12 0085 9 5681 7 4295 5 9150 5 3425 3 6450 mimin Remove All Magnifications Change Objective
208. microscope imaging device set 97 Main Program Window Important is the fact that after opening of an image saved in such a way in other programs for work with images the layer containing inserted objects will not be visible only the image without inserted objects will be displayed 2 Merge Objects with an Image Flatten Layers If you need the inserted objects in the image to be visible also in other programs for work with images for example if you need to insert the image to a text document or a presentation it is necessary to save the image with flattened layers In such case the layer above the image containing inserted objects will be merged with the image When you flatten layers all additional information image calibration the content of the table of measured values description of the image will be removed from the image When you open an image saved in such a way in other programs for work with images the image will be displayed with all inserted objects If you open such an image in the QuickPHOTO INDUSTRIAL 3 0 program it will no longer be possible to edit the inserted objects in any way The image will not contain information about the used calibration table of the measured values will be empty and the image s description will not be available To save images with flattened layers you can use all supported image types JPEG TIFF PNG as well as BMP Types of Files Supported by the QuickPHOTO INDUSTRI
209. must not be running while creating a report 155 Main Program Window To create new reports proceed as follows l Activate the Reports Create Report function 156 Select images in the Thumbnail Browser which you want to insert into a new report Create Report dialog box will open containing a list of available report templates Select a template which you want to use for creating of a new report and click the Create Report button Save New Report As dialog box will open Select the desired location enter the name of a new report and click the Save button Select Output Image Quality dialog box will open In this dialog box select the desired quality which will be used for insertion of images into the report Quality of the inserted images will affect the final size of the report file Following image quality options are available e Low Quality Small File Size for E mail images will be inserted to the report in a low quality The result will be a small report file suitable for sending via e mail e Medium Quality Medium File Size for Standard Printing images will be inserted to the report in a medium quality The result will be a medium size report file suitable for standard print e High Quality Large File Size for High Quality Printing images will be inserted to the report in a high quality The result will be a large report file suitable for high quality print Select Output Image Qualit
210. n be for better clarity hidden using the button To display the area size values again use the button It is also possible to change the area size using the green grasp points When dragging the corner grasp points with a mouse while holding the Shift key ratio of the area dimensions will be preserved 84 Main Program Window It is possible to move the chosen area by dragging the mouse while holding the left mouse button by the inside of the chosen area In case of need it is possible to cancel the Crop function by pressing the Esc key or by clicking the right mouse button rectangle defining the area will disappear Cropping itself is done by a mouse double click on the inside of the chosen area or by clicking on the 4 icon Only the chosen part of the image will remain The rest of it will be removed Copying Image or its Part to the Clipboard To copy the whole image displayed in the Display Window to the clipboard activate the Edit Copy option click the icon or you may use the Ctrl C key shortcut If you want to copy only a part of the image displayed in the Display Window to the poem clipboard activate the Edit Select Region option or click the icon first select the desired area for copying to the clipboard same way as was described in the previous paragraph Image Cropping To copy the chosen area to the clipboard activate the Edit Copy option click the icon or use the Ctrl
211. n or by clicking the icon In the displayed Circular Grid dialog box in the Spacing part enter the desired grid spacing Spacing can be defined in pixels and in current length units um or mm Color and thickness of the grid s lines can be set in the Line Options part Grid can be displayed in the image by clicking the OK button You can move the grid freely by dragging it by the cross in the middle of it Another activation of the Measurements Display Circular Grid option or repeated clicking the icon will cancel displaying of the grid in the image Note Circular grid is same as other objects being inserted into the image displayed in a layer above the image and if you save the image with the layers kept its full functionality will be preserved Circular Grid x Spacing Spacing 28 ym Spacing 507 pixels Line Options Line Thickness 3 pixels Line Color B Default values of the grid can be selected in Options of the program Edit Options gt Measurements Grid 124 Main Program Window 4 5 Phase Analysis Phase Analysis function enables to measure percentage amount of a particular color shade of grey the so called phase in the image It is possible to measure up to four phases in one image Important If you want to edit the image for example adjust the brightness contrast color balance histogram and so on it is important to make these a
212. n the Display Window e orthogonal grid e circular grid Displaying an Orthogonal Grid Tool for displaying an orthogonal grid can be activated by the Measurements Display Orthogonal Grid option or by clicking the icon In the displayed Orthogonal Grid dialog box in the Spacing part enter the desired grid spacing for the X or Y axis Spacing can be defined in pixels and in current length units um or mm Color and thickness of the grid s lines can be set in the Line Options part Grid can be displayed in the image by clicking the OK button You can move the grid freely by dragging it by the cross in the middle of it Another activation of the Measurements Display Orthogonal Grid option or repeated clicking the icon will cancel displaying of the grid in the image Note Orthogonal grid is same as other objects being inserted into the image displayed in the layer above the image and if you save the image with the layers kept its full functionality will be preserved Orthogonal Grid pai Spacing x A Ys 28 X 643 Ta 1907 Line Options Line Thickness 3 pixels Line Color ia n 123 Main Program Window Default values of the grid can be selected in Options of the program Edit Options gt Measurements Grid Displaying a Circular Grid Tool for displaying a circular grid can be activated by the Measurements Display Circular Grid optio
213. n your computer uninstall it 307 Troubleshooting FAQ 7 In case of using the OLYMPUS E system series digital SLR camera and the Grand AV USB 2 0 device for displaying of live view white USB device delivered with the previous versions of the program make sure the USB cables of the camera and the Grand AV USB 2 0 device for displaying of live view are not connected into the same USB controller Live view from the OLYMPUS digital camera on the computer monitor has a low quality it is checkered or snatchy If you use for displaying of the live view on the computer monitor an external USB device proceed as follows 1 Make sure the USB device is connected to the USB 2 0 port of your computer If your computer does not have any USB 2 0 ports install an additional USB 2 0 controller into your computer 2 Make sure your computer s performance corresponds with the minimal system requirements listed in the program specifications 3 Disconnect all cables from the camera except for the power cable 4 Activate the camera s menu In the SET submenu find the VIDEO OUT option and make sure the PAL option is active Further find the ALL RESET option and set it to OFF value When running the OLYMPUS digital camera control panel a message No video device is selected Live view won t be available pops up Device for live view displaying is probably not correctly configured in the QuickPHOTO INDUSTRIAL 3 0 program Conf
214. nabler IV Digital Microscope PathScan Enabler IV Digital Microscope X Image Resolution DPI Brightness Contrast PathScan Enabler IV digital microscope enables to acquire images of whole microscopic slides 239 Control Panels of Imaging Devices Important The images from PathScan Enabler IV device may have the size up to 63 Megapixels For work with images of this size it is necessary to perform the following settings of the QuickPHOTO INDUSTRIAL 3 0 program open the program Options and on the Miscellaneous tab Edit Options General gt Miscellaneous in the Maximal Image Size part in the Maximal Image Size in Megapixels drop down menu set the 64 or Unlimited value and confirm by clicking the OK button For scanning images from PathScan Enabler IV device it is necessary to have at least 2 GB of RAM installed in your computer Furthermore it is recommended to use the Microsoft Windows 7 or 8 Operating system QuickPHOTO INDUSTRIAL 3 0 program may in the Microsoft Windows XP operating system behave unstable when scanning full size images from PathScan Enabler IV device in the highest resolution Note After turning the device on it needs to warm up before first scanning This takes 60s Settings S Image Resolution DPI 7200 Brightness j _o j o Contrast _9 __ j o Gamma o
215. nd successive dragging of the mouse in the live view while holding the left mouse button will define a region which will be used as reference for the black balance Control Panels of Imaging Devices Display Hide Table of Measured Values if you click this button Table of Measured Values the Table of Measured Values will be displayed in the bottom part of the control panel If you click the button again the table will be hidden again Image Tab This tab contains control elements for setting of main camera s parameters Settings will apply to the live view as well as to the acquired images Pi Image Image Size 2592 x 1944 s Image Size from this menu you may select the size in which the images will be acquired 191 Control Panels of Imaging Devices Exposure Mode if the exposure mode is set to the Auto option exposure Auto e time is automatically set according to the illumination of the camera s manual field of view SFL Auto option represents automatic exposure mode for ieiki fluorescence imaging When using the Auto and SFL Auto modes the button Auto Exposure Lock will also be active This button locks the exposure time on the current value If the Manual option is active value of the exposure time is set by user using the control elements in the Exposure Time part Exp Correct this function enables you to lighten or darken the images when using the Auto and
216. ndicator of the set orientation of the ne timestamp If you double click into this field a Timestamp settings dialog box will open Preview Preview in this field preview of the timestamp is 17 10 2012 10 32 58 displayed the way it will be inserted into the acquired images except for the orientation option The text size is the same as will be displayed in the acquired images in the 100 1 1 display size Position the indicator shows the position where the timestamp will be inserted in the acquired images Timestamp Settings After clicking the Settings button or double click to the Orientation field the Timestamp settings dialog box will open 278 Control Panels of Imaging Devices Timestamp settings Format Font type Arial Font colour J Black v Font size 30 E V Bold Background colour O White v Transparent Date Format dd mm yyyy v Position Orientation left top Oright top Ovleft bottom O right bottom Preview 17 10 2012 10 32 46 Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text Profiles Tab All settings of the control panel can be saved i
217. ndow full screen You may return back to the original way of display by pressing any key by a mouse double click or a right mouse button click 7 Options Edit Options F11 option opens an Options dialog box with three main sections General Measurements and Hardware and it contains all options for settings of the program For application of the made settings click the OK button After clicking the Cancel button made settings will not be applied The whole setting of the QuickPHOTO INDUSTRIAL 3 0 program can be backed up into a file of the xml format by the Backup Settings button in the bottom part of the Options window If you need to restore the program settings you can do so by loading of the backup file after clicking the Load Settings button Configuration Wizard button opens the Configuration Wizard for more information see Configuration Wizard on page 25 Individual sections of the Options window will be described in the following chapters 7 1 Section General This section contains the following tabs Tab Default Folders This tab enables to set the folders which will be offered as default when saving images exporting measured values and saving reports This way you will not have to browse the whole directory structure of the computer to select the desired folder where you want to save the files Default folders can be changed anytime Standard dialog box for folder selection will open after clicki
218. ne by clicking the corresponding button of the profile on the Profiles tab Automatic adjustment of the control panel s elements will take place 281 Control Panels of Imaging Devices 282 Modules Program QuickPHOTO INDUSTRIAL 3 0 contains extensive functions in the form of built in modules Other optional modules can be added to the program 1 Timer Built in Module for Time Lapse Image Capturing QuickPHOTO INDUSTRIAL 3 0 is equipped with the Timer module which is designed for automatic image capturing in the defined time interval the so called time lapse image acquisition This module can be activated by the Modules Timer option or by clicking the icon in the Toolbar Timer dialog box will be displayed along with the control panel of the active imaging device Timer dialog box contains a Capturing tab only in a default state If you have activated a computer controlled illuminator in Edit Options gt Hardware Illuminators the Timer dialog box will also contain a second tab Illumination After setting of the desired parameters described in the following chapters run the time lapse b image capturing by clicking the putas Time lapse image capturing can be terminated at any time by clicking the _ 5 button In the bottom part of the Timer dialog box there are the following information displayed Time until Next Capturing Number of Captured Images and Number of Remaining Images Note If the Ti
219. ned manually by the fields at the bottom of the dialog box Confirm the settings by clicking the OK button 276 Control Panels of Imaging Devices Crop borders Cropped borders Left 0 px Right 0 px oe o pe paian ojs oe Selected area 720x576 Device Settings Tab Jere Input select the input type of the analog signal depending on the currently S Video used input connector You can select S Video or Composite see figure Composite options S Video Composite It is possible to automatically insert into the acquired images date and time of their acquisition the so called timestamp The timestamp will after acquisition of the image Timestamp Tab 2l Control Panels of Imaging Devices become a solid part of the image and it is not possible to remove it from the image other way than by cropping it Ee Timestamp Timestamp Insert Orientation Preview Position left top Disabled Date Aire ace 17 10 2012 10 32 58 Timestamp Timestamp this option turns the Timestamp function on off Disabled Enabled Settings 1f you click this button a dialog box for setting of timestamp parameters will open Insert Insert by checking of individual options it is possible to define which es f the ti ill be inserted into the acquired i time components of the timestamp will be inserted into the acquired images Seconds Orientation Orientation this field serves as an i
220. nels of Imaging Devices Timestamp settings Format Font type Arial Font color Black vi Font size 18 V Bold Background color E White i C Transparent Date format dd mmjyyyy v Position Orientation top left top right Abc oO bottom left bottom right qy Preview 12 10 2012 1 58 19 PM Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text Profiles Tab All settings of the control panel except for the settings of the Shading correction function can be saved into one of the four profiles and at any time loaded again H H Profiles Profiles a bell Save to profile Default 2 BS Default profile E Bi Default 3 Default 4 253 Control Panels of Imaging Devices Save to profile if you click this button a dialog box will be displayed for saving of the current control panel s settings into one of the profiles Default profile if you click this button all elements of the control panel will be set to the default values Saved profiles will not be affected in any way by this action Saving of the Control Panel s Settings into the Profile To save the settings into the
221. nes measuring of the length diameter and angle of an arc measuring of the phase analysis l Brinell hardness test and Vickers microhardness test It is also possible to insert a rectangular or circular grid of defined dimensions into the image 114 Main Program Window Note To use the functions for measurements it is necessary to calibrate the QuickPHOTO INDUSTRIAL 3 0 program first for more information see Opening Information about Calibrations on page 33 To achieve correct measuring results it is necessary to have set the corresponding active calibration for the microscope imaging device set in use for more information see Setting an Active Calibration on page 58 4 1 Preparing for Measuring Selecting Magnification which Was Used for Acquiring of the Image Before you begin measuring first you must assign the corresponding microscope magnification which was used when acquiring the image intended for measuring Proceed as follows 1 Display the desired image in the Display Window 2 Inthe Toolbar choose from the Objective or eventually Zoom menu the corresponding magnification In the list you can only choose from those values for which calibration was created If your microscope is not equipped with a magnification changer or a zooming unit there will be after you choose an objective in the Zoom menu automatically set the value 1 After this step functions for measuring will be ac
222. ng of two or four images next to each other Compose New Image from Layout of Displayed Images dialog box will open 161 Main Program Window Compose New Image from Layout of Displayed Images x This function composes a new image from visible parts of images displayed in current layout of main program window Note Measurements will not be available in the composed image Image Size Background Color No Space Space Defined in Pixels Space Defined in Percents of Image Width Borders of Particular Images No Borders Border Thickness Margins of Composed Image No Margins Margins Size This dialog box consists of the following parts Composed Image part contains two options In the Image Size drop down menu there are the available sizes of the composed image By the Background Color option you can choose the background color of the space between the images and the color of the borders around them In the Space among Images part you can choose whether the individual composed images should be offset from each other and you can define the size of the space between them The space will have the color selected in the Background Color part In the Borders of Particular Images part you can select the width of the black frame around individual images In the Margins of Composed Image part you can select the size of borders which will be added around the inserted images The borders will have t
223. ng about the USB Serial Port COM In the part Remote Trigger Type select the type of the used remote trigger Trigger with two buttons for example the two button foot switch is designed for image acquiring and for capturing of video recordings using the optional module for video recording Functionality of the remote trigger can be verified by pressing its button s If the small square s change color to green after you press the button s and they turn back to black after you release the button s the remote trigger is set correctly and it is ready to be used Click the Next button 30 Initial Configuration of the Application Configuration Wizard QuickPHOTO Remote Trigger INDUSTRIAL In this step a remote trigger optional accessory can be set 3 0 L Select a COM port to which is the remote trigger CoM1 7 connected 2 Verify the remote trigger s functionality by pressing its button s The black square s should turn green Remote Trigger Type Single Button Trigger 8 Two Button Trigger Finish the configuration by clicking the Finish button Then QuickPHOTO INDUSTRIAL 3 0 program will run automatically 31 32 Initial Configuration of the Application Calibration of the Program for Measurements 1 Opening Information about Calibrations Before it is possible to start measuring in the QuickPHOTO INDUSTRIAL 3 0 program it is necessary to calibrate the program for a particular microscope imagin
224. ng objects displayed in the live view Live View Tab This tab is designed for setting of live view parameters T Live View Live View Resolution 1296 x 968 Subsampling 2x2 s Frame Rate FPS 21 9 Image Quality Standard High Frame Rate High Lower Frame Rate Special Remove Moir Live View Size Original 1 1 Fit to Window Rotate by 180 ate Cross Grey Scale Performance Test Live View Resolution from this drop down menu you may select the size in which the live view will be displayed 206 Control Panels of Imaging Devices Frame Rate displays how many images per second Frames per Second FPS is the camera currently displaying in the live view Image Quality Standard High Frame Rate option displays the live view with the highest possible frame rate High Lower Frame Rate option improves the quality of the live view at the cost of possible frame rate decrease Use the Special Remove Moire option in case disruptive stripes or possibly squares on the background are visible in the live view This phenomenon can appear with some types of illumination Live View Size here select whether you want to display the live view in the original size 1 1 Original 1 1 option or if you want to fit its size to the control panel s window If the Fit to Window option is active a decrease in the frame rate may appear
225. ng of computer controlled illuminators In the uminator Settings part check the Use PC Controlled Illuminator option and from the Control Unit 187 Main Program Window drop down menu select the control unit of the used illuminator Correct functionality of the illuminator can be verified by clicking the Test Device button 188 Note Only those control units whose support was chosen during program installation will be displayed in the list If your control unit is not available in the list exit the QuickPHOTO INDUSTRIAL 3 0 program and run the program installation from the installation CD again On page Installation Type Selection select the Update Existing Installation Add Components option and on page Select Additional Components PC Controlled Illuminators choose from the list a control unit whose support you want to add Options 4 General Default Folders Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices Analog Video Source Remote Trigger Magnification Encoders Tlluminators x If using the PC controlled illuminator activate it by checking the respective checkbox and select the type of a control unit Illuminator Settings greseesosossesesosossosceseocoscossocossosessessessseossossesessessse Control
226. ng the Change button Change of the folder can also be done by typing in of 164 Main Program Window the desired path into the corresponding field If the selected folder does not exist it will be created Options x 7 p Folders _Default Folders ee Image Names amp Saving Default Folder for Saving Acquired Images Sound C Program Files QuickPHOTO INDUSTRIAL 3 0 Images Change Language Do Not Show This Window Default Folder for Exporting Measured Values Annotations C Program Files QuickPHOTO INDUSTRIAL 3 0 Results Change Appearance and Layout Miscellaneous Default Folder for Saving Time Lapse Images ee ee 4 Measurements C Program Files QuickPHOTO INDUSTRIAL 3 0 Sequences Change Units Scale Bar Default Folder for Saving Reports Sizes Colors C Program Files QuickPHOTO INDUSTRIAL 3 0 Reports Change Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Bacup settings Gi Load Settings Configuration Wizard OK Cancel Tab Image Names amp Saving This tab contains the following parts Names of New Images part contains preferences for naming of new images Default name of all new images acquired by the imaging device can be determined by typing in of a text string into the Acquired Images field Serial number will automatical
227. ng the left mouse button you can change the size shape of the object Measured values will be recalculated Relocating the Label with the Measured Value s in the Image In order to edit inserted objects select them first for more information see Selection of the Measuring Objects for Editing on page 136 You can relocate labels with measured values in the image as you please Move the mouse cursor over the label with the measured value s of the selected object Cursor will change to In this moment you can by dragging the mouse while holding the left mouse button relocate the label with measured value s to the desired area in the image Label with the measured value s will be connected with the measuring object by an auxiliary line Type and shape of the auxiliary lines will be automatically set depending on the current label with the measured value s position due to the location of the measuring object L1 297 um L1 298 um 138 Main Program Window Default color and thickness of the dimension and extension lines and leaders 1s predefined on the Sizes Colors tab in Options of the program Edit Options Measurements gt Sizes Colors You may change the color and thickness of these lines for a specific image in the Table of Measured Values on the Measurement Settings Image Name tab in the Dimension and Extension Lines and Leaders patt Deleting Measuring Objects In order to edit inserted object
228. ng the slider you may set the level of correction interactively Using the Shading Correction Function Important Shading correction gives good results only if all the settings of the microscope and the camera are the same while acquiring images as when capturing a calibration mask If you change the conditions change of magnification change of illumination change of image size and so on it is necessary to acquire the calibration mask again To use the Shading Correction function it is necessary to create a calibration image the so called mask first Proceed as follows Transmitted Light 1 Focus the microscope using the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Remove the specimen and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view 211 Control Panels of Imaging Devices is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Auto WB button automatic white balance On the Jmage tab set the desired image size 5 Switch to the Shading Correction tab and select the Enabled option In the umination Type part select the Transmitted option Then in the Calibration Mask part click the Capture Mask button Preview of the mask will be displayed in the control panel 6 Plac
229. nge Utility from the Start Programs QuickPHOTO QuickPHOTO INDUSTRIAL 3 0 OLYMPUS CAMEDIA and SP Series Camera Change 5 Click the OK button in the OLYMPUS SP and CAMEDIA Series Camera Change Utility window When asked turn the camera off and back on Finish the change by clicking the OK button Sign off from the administrator s account After you have signed in to your standard user account the QuickPHOTO INDUSTRIAL 3 0 program should be ready to work with the new camera U See ae The whole procedure change of camera must be repeated each time before the first use of the new camera If you decide to use the original camera you do not need to repeat the above mentioned procedure 5 3 Adding the Support of Magnification Encoder or a PC Controlled Illuminator If you want to install the support of a new device into an already installed QuickPHOTO INDUSTRIAL 3 0 program insert the program installation CD to the CD ROM driver and run the installation On page Installation Type Selection select the Update Existing Installation Add Components option and select from the list the desired devices whose support would you like to add Continue according to the wizard s guidelines 23 24 Installation Initial Configuration of the Application First Run of the Program When you run the QuickPHOTO INDUSTRIAL 3 0 program for the first time a wizard will open which will help you to configure the program co
230. nnotation of Interesting Areas 90 318
231. nt Mode Selected tool remains active until deactivated Single Measurement Mode Single measuring object will be inserted upon tool activation Display Mode Measuring Objects Control elements in the top part of this tab are designed for selection of measurands which will be measured by individual measuring tools and for text labeling of individual measurands Measurands to be measured should be checked To change the label rewrite the appropriate field in the Label row Measurement Mode part offers two possibilities e Continuous Measurement Mode if this option is active default option the selected measuring tool will remain active until turned off That way you can proceed with repeated measurements in the live view without the necessity to manually activate the same measuring tool for each measurement 205 Control Panels of Imaging Devices e Single Measurement Mode if you activate this option the measuring tool will be after one performed measurement automatically deactivated In the Display Mode Measuring Objects part there are two options available e Keep Displayed Only the Last Measuring Object if this option is active default option only the last measuring object will always be displayed in the live view When new measuring is being performed the previous measuring object will always be deleted from the live view e Keep Displayed All Measuring Objects this option will leave all measuri
232. ntain any other images Processed will be all images from the selected folder therefore be careful not to forget any unwanted images among the images intended for processing This option enables processing of virtually any number of images Click the Next button 289 Modules 4 j5 Time Lapse Video X Selection of Image Source This wizard will help you create video file from time4apse images On this page select source of images for processing and click the Next button Process Images Selected in the Thumbnail Browser Select Saved Files for Processing Up to 2000 Files Select the Whole Folder for Processing Next page of the wizard Video Properties enables to set parameters of a new video file There are two options affecting the speed of the captured action available e Interval between Frames this option enables to set the time interval between images This option is useful for example if you want to have control over the speed of the projected action compared to reality e Frame Rate this option enables to create a video file with the desired length or fluency To create a fluent video file the minimum value of 25 images per second FPS is recommended According to the set parameters the Video Duration will be calculated In the Video Resolution part you may select the size of the resulting video file in pixels The larger size you select the larger will be the resulting video file
233. nter Spot ful SLR camera this menu is accessible only if there is the A mode selected in the Live view mode menu See the user s guide of the camera for more details Live view mode E 330 SLR only the E 330 SLR camera is equipped with two sensors In the B mode the same sensor is used for capturing images and also for displaying the live view sensor with higher resolution but automatic focusing is not available due to the construction of the camera Colors Tab On this tab you can set several parameters influencing the quality of images Color mode Shading compensation Gradation NATURAL v Normal v Contrast Sharpness Saturation lo vlo o v 246 Control Panels of Imaging Devices Selene Color mode here you can set the color mode Changes will reflect interactively in the live view The default option is VIVID Contrast Contrast contrast of images can be adjusted by this menu Shading comoensation Shading compensation shading compensation can be activated in this menu See the user s guide of the camera for more details Sharpness Sharpness sharpness enhancement can be set in this menu aan Gradation color gradation mode can be selected in this menu Changes will reflect only in the captured images Saturation Saturation value of color saturation can be set in this menu Live View Tab This tab contains the controls intended for setting the live view The available parameters
234. nto one of the four profiles and at any time loaded again mo Frofiles Profiles lelsavetaprofie Save to lelsavetaprofie a 1 one a fm 3 oon 4 IE Defaut profile Default O IE Defaut profile 279 Control Panels of Imaging Devices IE save to profile Save to profile if you click this button a dialog box will be displayed for saving of the current control panel s settings into one of the profiles W default profile Default profile if you click this button all elements of the control panel will be set to the default values Saved profiles will not be affected in any way by this action Saving of the Control Panel s Settings into the Profile To save the settings into the profile proceed as follows 1 Set the desired parameters of the control panel 2 On the Profiles tab click the Save to profile button Save Profile dialog box will open Save Profile 1 Select the profile you want to save current camera control panel settings to Available profiles E i Default 1 Default 2 E E Default 3 Default 4 2 Type a new profile name 3 In the Available profiles part select by clicking the corresponding button one of the profiles you want to save the current control panel s settings to 4 Type in anew profile name into the Profile name field 5 Confirm by clicking the OK button 280 Control Panels of Imaging Devices Loading a Profile Loading of a desired profile can be do
235. nts can be defined 3 0 Scale Bar Type mee Fuler 7 Measurands Next page of the wizard is used to select the measurands which will be measured by individual measuring tools length diameter radius perimeter area angle and the text label of individual measurands row Label Select the measurands which are supposed to be measured by checking them To change the label rewrite the appropriate text in the line Label If the option Display Measurement Numbers in Images is checked corresponding serial number of the measurement will be displayed by each measured value in the image These settings will become default for the whole program and they will be valid for all newly acquired images To continue click the Next button 29 Initial Configuration of the Application Configuration Wizard QuickPHOTO Measurands N D US TRI AL In this step measurands and labels for each measurement type can be selected 3 0 Length Diameter Radius Perimeter Area Angle Distances Lengths Cirde Rectangle Polygon Ellipse Angle Arc Label L D Display Measurement Numbers in Images Remote Trigger This page is used for setting and verification of the remote trigger s functionality Remote trigger 1s an optional accessory which can be connected via serial or USB port If you want to use the remote trigger check the Use Remote Trigger option and select the COM port to which it is connected in case of the USB trigger we are talki
236. of Imaging Devices mouse button you may define an area which will be used as reference for black balance Default BB button is used for restoration of the default black balance This function 1s designed primarily for fluorescence imaging Measurements Tab Control panel of Lumenera INFINITY camera is equipped with functions for measuring in live view Thanks to this function it is not necessary to acquire the image before measuring This tab contains control elements for measuring in the live view Measurements Active Calibration CCD Camera Upright Microscope Objective 10 z Zoom v Eo H A 10 14 Vv g ol 1 I x 10 re 14 ix ev Convex Acute Angle Convergent Obtuse Angle 2 a swe Active Calibration in this part the name of a calibration CCD Camera Upright Microscope which is set as active and is used for measuring in the Objective 10 QuickPHOTO INDUSTRIAL 3 0 program is displayed To Zoom activate functions for measuring in the live view first select value of the current microscope magnification from the Objective and Zoom menus If you are using a magnification encoder these values will be filled in automatically In the Measuring Tools part the following measuring tools are available 195 196 Control Panels of Imaging Devices Insert Horizontal Scale Bar after you click this button a horizontal
237. ol panel If needed the performance test can be launched manually by clicking the Speed adjustment button on the Options tab Note There should be no other application with high demand on computer s performance running during the performance test otherwise the camera will work slower than it could Image Tab This tab contains control elements for setting of main camera s parameters Settings will apply to the live view as well as to the acquired images PS Image Live view Quality Capture Images in Exposure Region Focus Region 1280 x 960 Live View Resolution 1280 x 960 100 45 custom aana KJ EN E e We Full Resolution 2560 x 1920 Exposure Mode Exposure Time Exp Correct Color Mode Cross O30 3 Auto AEL r Color gt sine 1 215 0 v 5 C Display Central Cross O1 anual Grey Scale E Capture Ctrl Enter after clicking this button an image will be acquired and Capture automatically transferred to the computer Full Screen this button is used for displaying of the live view full screen Full Screen Using this type of display you can control the program using the Capture Ctrl Enter and the Exit Full Screen Mode buttons placed in the upper part of the screen ce Connect this button is enabled only when the camera is not correctly Connect connected Click this button to re establish communication with the camera 222 Control Panels of Imaging Devices Auto WB this button act
238. om the list a control unit whose support would you like to add Options 4 General Default Folders Image Names amp Saving Sound If using the magnification encoder activate it by checking the respective checkbox and select type of the control unit Language Upright Inverted Microscopes Do Not Show This Window Use Objective Encoder Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Stereomicroscopes Measurands Hardness V Use Zoom Objective Encoder Other Settings Control Unit SZX2 ZB16 ZE SZX2 ZB10 ZE 4 Hardware Test Device Imaging Devices Objective Encoder Device SZX2 2RE16 SZX2 2RE10 Analog Video Source Remote Trigger Recalibrate Zoom Encoder Magnification Encoders Illuminators Ie Backup Settings a Load Settings J Configuration Wizard OK Cancel Adding a New Calibration with the Magnification Encoder for Stereomicroscopes Adding of a new calibration shall be done in a similar manner as described above for more information see Adding a New Standard Calibration on page 35 with the following differences e In point 3 of the guide named Add Calibration Calibration Name on Magnification Encoder Selection page check the Zoom and Objective Encoder for Stereomicroscopes 53 54 Calibration of the Program for Measurements option if you have installed only the z
239. ompletion Lumenera INFINITY Digital Cameras 18 Description of the Graphical User Interface 62 Display Window 79 Menu Bar 64 Navigator 78 Status Bar 80 Table of Measured Values 79 Thumbnail Browser 76 Toolbar 71 Installation Completion OLYMPUS Measurements 114 CAMEDIA and SP Series Digital Cameras 22 Installation Completion OLYMPUS E 330 SLR Digital Camera 21 Installation Completion PathScan Enabler IV Digital Microscope 20 Leadtek WinFast VC100 XP Device 12 Lumenera INFINITY Digital Cameras 10 OLYMPUS Digital Camera Device Drivers 16 OLYMPUS Digital Cameras E system CAMEDIA and SP series 1 PathScan Enabler IV Digital Microscope 1 QuickPHOTO INDUSTRIAL 3 0 Program 17 Switching the Camera to the PC Control Mode 14 L List of Key Shortcuts 297 Brinell Hardness Test 133 Count Objects 121 Displaying a Grid of Defined Dimensions 123 Editing of the Measuring Objects 136 Export Measured Values to Microsoft Excel Continuously 147 Exporting Values from the Table of Measured Values 144 Measure Angle 121 Measure Arc 122 Measure Circle 119 Measure Distance Between 2 Parallel Lines 122 Measure Ellipse 119 Measure Line Segment 119 Measure Polygon 120 Measure Polyline 120 Measure Rectangle 119 Phase Analysis 125 Preparing for Measuring 115 Scale Bar 117 Standard Measuring Tools 118 Vickers Hardness Test 135 Work with the
240. ongle is flashing and the QuickPHOTO INDUSTRIAL 3 0 program when run announces that the dongle was not found Properly working USB dongle is supposed to lit at all times After you have installed new device drivers of the dongle it may start flashing for about one minute Do not run the QuickPHOTO INDUSTRIAL 3 0 program during this time If you do not follow these instructions the dongle may not stop flashing after one minute is over In this case disconnect the dongle and connect it again or possibly restart the computer If the procedure does not help it is necessary to connect the dongle to a different computer with installed dongle device drivers Wait about one minute During this time do not run any programs After this time period is over the dongle should stop flashing and it should lit again Then you may connect the dongle to the original computer and start using it with the QuickPHOTO INDUSTRIAL 3 0 program 311 Troubleshooting FAQ It is not possible to play the video file created using the Time Lapse Video module Install a codec from the QuickPHOTO INDUSTRIAL 3 0 program installation CD from the Codec folder to the computer on which you are not able to play the created video file You may also download the codec for free from the website www promicra com downloads php I have not found an answer to my question Where can I get more information Try to look for your question in the FAQ section on the www promicra co
241. oom encoder without the objective encoder the option Zoom Encoder for Stereomicroscopes will be displayed From the Microscope Type drop down menu choose the corresponding type of the stereomicroscope in use and continue by clicking the Next button You will be prompted to set the zooming unit to the 0 8 position to calibrate the zoom encoder Set the required zoom value and continue by clicking the OK button Add Calibration CCD Camera Stereomicroscope x Magnification Encoder Selection Select magnification encoder you want to use for calibration CCD Camera Stereomicroscope Only encoders configured in Edit gt Options gt Magnification Encoders menu are available Select Magnification Encoder No Magnification Encoder Manual Magnification Assignment Mode Zoom and Objective Encoder for Stereomicroscopes Microscope Type 52X16 X A stereomicroscope objective encoder is active To change the settings go to Edit gt Options gt Magnification Encoders menu lt Back Next gt Cancel If you have installed and also activated the objective encoder for stereomicroscopes in the program Options a page First Objective Position and Magnification Assignment will be displayed Continue according to the displayed instructions for assigning of the first objective magnification with the corresponding position of the objective encoder In the next step do the assigning for the second objectiv
242. oose how you would like to count the proportions of individual phases Calculate the Phase Proportion Relative to Area of Defined Regions in the Image Overall Area of All Phases If the mode Use the Whole Image Area for Phase Analysis 1s active there are two options available e Whole Image Area as a base 100 for the calculation of the phase amount will be used the whole image area e Overall Area of All Phases as a base 100 for calculation of the phase amount will be used an area indicated by all phases together Area which will not be indicated in the image will not be reflected in the calculation If the mode Phase Analysis Inside Defined Region s of the Image 1s active there are two options available e Area of Defined Regions in the Image as a base 100 for the calculation of the phase amount will be used the area of unification of all defined regions e Overall Area of All Phases as a base 100 for the calculation of the phase amount will be used the area indicated with all phases together Neither unmarked area inside regions nor area outside regions will be reflected in the calculation If the mode Exclude Unwanted Region s of the Image from Phase Analysis 1s active there are three options available e Whole Image Area as a base 100 for the calculation of the phase amount will be used the whole image area e Whole Image Area Excluding Def Regions as a base 100 for calculation of the
243. options 1s active images of all types will be saved with the flattened layers For images of the PNG and BMP type will the layers always be automatically flattened during automatic image saving In the Default JPEG Quality part it is possible to preset the default quality for saving of images of the JPEG type This setting will be used for the automatic saving of JPEG type images and it will also be used as the default setting for manual image saving Tab Sound This tab offers the option of setting the sound played during acquiring of images If the Play a Sound when Image Has Been Acquired wav file option is checked a sound file of the wav type will be played when acquiring an image To choose the sound file click the Change button and select the desired file After clicking the button the selected file will be played Options x 4 General Default Folders Image Names amp Saving V Play a Sound when Image Has Been Acquired wav file Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Grid Measurands Hardness Other Settings 4 Hardware Imaging Devices OLYMPUS SLR Consumer Cameras Analog Video Source Remote Trigger Magnification Encoders Illuminators Sound C Program Files QuickPHOTO INDUSTRIAL 3 0 trigger wav gt Change Bacup settings Gi Loadsettings Configuration Wizard
244. osition If you place the cursor above the frame s edge or above one of its corners the cursor will change shape and by dragging the mouse while holding the left mouse button you may change the size of the frame as you please By clicking the Center button you may place the frame to the center of the camera s field of view Using the Display Hide button it is possible to hide or display the frame If the frame is hidden the selected region is still being used for measuring of exposition The frame is only not visible in order not to disturb in the live view To define your own exposure region click the Custom button move the cursor to the live view and then by dragging the mouse while holding the left mouse button insert the frame of a desired size Note If the frame of the exposure region will change its color to red it means that the camera s automatics have reached the limit value of the exposure time The range of exposure times is in the automatic mode smaller than in the manual mode White Balance using this function you may adjust the white balance 1 Auto wB Clicking the Auto WB button will activate the automatic white balance function This function uses the whole image area for white balancing It 1s best to perform the white balance when the camera is capturing a white area If you click the Region WB button and then drag the mouse in the live view while holding the left mouse button you may define a region whic
245. ositions button in this dialog box which opens the Change Objective Positions dialog box which enables to make changes in assigning of the objectives to the positions of the magnification encoder 55 Calibration of the Program for Measurements Table of Magnifications Calibration CCD Camera Stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification SZX 16 Objective Position 0 7 0 8 1 1 25 1 6 2 25 aa 0 5 2 i 1 5 Change Objective Positions 4 il gt Graph of Calibration Values 1 Calibration Value 1 mm ie Zoom Magnification OK Calibration of Individual Magnifications Using the Magnification Encoder for Stereomicroscopes When calibrating the individual magnifications proceed in a similar manner as described above for more information see Standard Calibration of Individual Magnifications on page 38 Major difference from the standard calibration of individual magnifications is the automatic filling of the Zoom Magnification Changer Magnification field If you have also activated the objective encoder values of the objective magnification will also be automatically filled in the Current Objective Magnification field on 1 Magnification Selection page in the Calibrate New Magnification Calibration Calibration name guide 56 Calibration of the Program for Measur
246. ow File Edit Image View Measurements Annotations Imaging De a a Open Ctrl O Open Recent gt fe Save Ctri S Ed Save As Shift Ctr S i Save All Ctrl Alt S E Save All As Shift Ctri Alt S i Export Measured Values from Selected Images Ctrl M ky Export Measured Values from All Images Ctri Alt mM Close Selected Images Ctrl W Close All Images Ctrl Alt w eh Open Imaging Device Control Panel F4 amp Print Ctrl P Exit Alt X Menu Edit The following options are available in the Edit menu Undo will take back the last performed action It is possible to take back as many consecutively performed actions as you wish Option Redo will again perform the action taken back using the Undo function Option Copy inserts an image or its chosen part into a clipboard Option Paste will insert the content of the clipboard as a new image Option Se ect Region 1s used for selection of a part of an image to be copied into a clipboard Change of color and thickness of the lines of the objects being inserted can be done using the options Object Line Color and Object Line Thickness Option Compose New Image from Layout of Displayed Images will compose the images displayed in the Display Window for layout of 2 or 4 images next to each other and the result will be inserted as a new image For a more detailed description see Compose New Image from Layout of Displayed Images on page 161 Option Restore Original Ima
247. ow in the Zable of Measured Values If you activate the function Count Objects New Group again in the same image further counted objects will be indicated with a cross of a different color and in the Table of Measured Values there will be another line displayed with the number of elements of another group of objects If you want to add further objects to one of the already existing groups of objects mark one of the elements from this group using the Select tool or mark the corresponding row in the Table of Measured Values by clicking on it and activate the option Measurements Count Objects Add Objects to the Current Group or click on the arrow on the right next to the icon and select the option Add Objects to the Current Group Now you can continue in counting of objects from the selected group If you want to change the color or size of the crosses of the whole group of objects mark one of the elements of this group using the Se ect tool or select it by clicking the corresponding row in the Table of Measured Values and using the options Edit Object ba al Line Color and Edit Object Line Thickness or using the 7 icon select the desired parameters Measure Angle Tools for angle measurement can be activated by the options Measurements Angle gt Arbitrary Angle Angle from the X Axis Angle from the Y Axis Angle Defined by 2 Line Segments or by clicking on the arrow on the right from the icon and b
248. pen option by clicking the icon or by a Ctrl O key shortcut In the opened Open dialog box you may select one or more images and open them all at the same time Another possibility for opening of images is to drag them with a mouse into the Display Window drag and drop for example from the Windows Explorer or another file manager Thumbnails of the opened images will appear in the Thumbnail Browser together with their names types and sizes Menu File Open Recent offers up to ten last used images on a list for opening Ga Open Ctri 0 Open Recent gt 82 Main Program Window 3 2 Image Editing Note All editing is always done to the currently displayed image in the Display Window Options for image editing may be activated from the nage menu Some of these options may also be activated using the icons in the Toolbar Menu Image contains the following options for image editing Crop Rotate Clockwise and Counter Clockwise by 90 Flip Vertically and Mirror Horizontally Resize Automatic White Balance White Balance Defined by Point Filters Sharpen Blur Median Filter Negative Grey Scale Black Balance Defined by Point Adjust Brightness Contrast Gama Adjust Colors Adjust Histogram Detailed description of individual functions is in the following paragraphs Cropping Image Function Crop is used for removal of undesirable image areas Activate Crop function from the Image Crop menu or by
249. played cross will not be visible in the acquired images 220 Control Panels of Imaging Devices Grey Scale turns on off the grey scale mode Grey Scale 3 IDS uEye Digital Cameras is uEye UI 1480 C Digital Camera a Options Shading Correction RF Timestamp E mo Profiles Live View Quality Capture Images in Exposure Focus Region KA 1280x950 v 960 Live View Resolution 1280 x 960 100 iem aceon KA EN ect e We O Full Resolution 2560 x 1920 Exposure Mode Exposure Time Exp Correct Color Mode Cross O30 Cente S Re uto act Color O Manual l O Grey Scale Display Central Cross O1 Display Hide Display hide The control panel is able to control several models of IDS uEye digital cameras Type of the connected camera is determined automatically at the startup of the control panel If more than one IDS uEye digital camera is connected to the computer a dialog box will be displayed at the startup of the control panel for selection of a camera which is supposed to be connected The control elements are located in five tabs 221 Control Panels of Imaging Devices Speed Adjustment At the very first run of the control panel a performance test will be launched and the camera s frame rate FPS will be adjusted according to the computer s performance This performance test is performed only once and it will not be launched at next run of the contr
250. played next to each other in the Display Window look for example like this one Print Window Print window consists of several parts e Preview e Printer tab e Page Layout tab e buttons in the bottom part 110 Main Program Window Print x Preview Name HP Color LaserJet 2605 UPD PS Status Ready Type HP Universal Printing PS Color Mode Color Grey Scale Portrait Landscape Copies Copies cm inches Margins Top 0 43 Right 0 47 lt Bottom 0 43 cm Minimal Margins Paper Size A4 Printer DPI 600 BS Default Settings Print Cancel Apply Part Preview In the right part of the Print window is displayed the page Preview as it will be printed out Settings made are continuously updated in the Preview field It is then possible to make required print settings interactively Under the Preview there are displayed information about the currently selected paper format and the set print quality units DPI Dots Per Inch Printer Tab In the Printer part choose the desired printer from the Name drop down menu which contains the list of available printers Properties button opens a dialog box with advanced settings for the chosen printer 111 Main Program Window Orientation part contains two options of the page orientation Portrait and Landscape To print one and four i
251. profile proceed as follows 1 Set the desired parameters of the control panel 2 On the Profiles tab click the Save to profile button Save Profile dialog box will open Save Profile 1 Select the profile you want to save current camera control panel settings to Available profiles E i Default 1 Default 2 E E Default 3 Default 4 2 Type a new profile name 3 In the Available profiles part select by clicking the corresponding button one of the profiles you want to save the current control panel s settings to 4 Type in anew profile name into the Profile name field 5 Confirm by clicking the OK button 254 Control Panels of Imaging Devices Loading a Profile Loading of a desired profile can be done by clicking the corresponding button of the profile on the Profiles tab Automatic adjustment of the control panel s elements will take place 7 OLYMPUS DP72 Digital Camera OLYMPUS DP72 control panel J Capture Live image i Color balance g Shading correction E2 Timestamp bell Profiles 5 Image Binning Accumulation multiple images i E Size 4140x3096 v off v Mode Disabled v Count Full screen Exposure time IS sens Exposure mode Exposure region Time 1 1505 Correction 0 vi OManual 30 i 200 v 3 duto O1 O SFL Auto O0 1 OLYMPUS DP72 isa digital camera with the 2 3 CCD chip The chip which incorporates 1 45 million effective pix
252. pulled out Its anchoring may be done by clicking the button T1 Main Program Window FH Thumbnail Browser Image Count 4 Selected 1 2776 x 2074 sample2 jpa 2560 x 1920 1 4 Navigator In the left bottom part of the Main Program Window there is a Navigator displayed with the current image In the Navigator window there is a framed area of the image which is visible in the Display Window at the current display size Display of the current image can be enlarged or reduced with the mouse wheel slider or with the or buttons under the Navigator window It is possible to move the frame by clicking the left mouse button inside the frame and by moving the mouse while holding the left button By moving with the frame you will achieve the desired display of a particular image area in the Display Window 78 Main Program Window 1 5 Display Window Display Window is designed for work with images In case you need to work with more than one image this window may be divided into two or four parts using the Layout Two Images or Layout Four Images options Switching to the default mode for one image is possible by the Layout Single Image U option These functions can also be activated by the corresponding options from the View menu 1 6 Table of Measured Values In the bottom part of the Main Program Window there is the Table of Measured Values This table contains a tab tabs with values mea
253. quence in the Number of Captured Images part and how many images remain to be captured in the Number of Remaining Images part Timer x Caphsing Number of Images Number 10 Time Interval Interval between Images Hours Minutes Seconds 0 10 lt sS Y Store Images in the Main Program Window in Folder C mages S File Name Timer Time until Next Capturing 00 00 00 Number of Captured Images l 0 Number of Remaining Images 285 Modules 1 2 Tab Illumination If you have activated control of the computer controlled illuminator in Edit Options Hardware Illuminators the Timer dialog box will contain also the umination tab This tab enables to set turning off the illumination between capturing images in order to avoid undesirable long term effect of the illumination on the specimen The illumination will always be turned on only for the time period necessary for the image capturing and then it will be turned off To turn off the illumination between individual image capturing check in the Control during Capturing part the Turn off Illumination between Captures option The following information are valid for the checked option Turn off Illumination between Captures Turn on Illumination before Capturing option sets an advance for turning on the illumination before capturing each image It is recommended to set the advance of at
254. r fey Backup Settings Ga Load Settings Configuration Wizard OK Cancel In the Toolbar part there are two options available If you check the Display Toolbar Buttons with Captions option all icons in the toolbar will change into buttons containing also their names If all buttons do not fit on the monitor arrows Fl which will offer the remaining buttons in the drop down menus will also be displayed in the Toolbar Example of the displayed buttons is on the following picture e 09 Image Information 7 Undo Redo g e T E E Insert Horizontal Scale Bar Line Segment Elipse Polygon Display Circular Grid Export Measured Values to Microsoft Excel Continuously 7 eee ees o 2 5 Text Line Arrow Rectangle Cirde Elipse Polygon Freehand 7 5 Oy a Crop Rotate Clockwise by 90 3 Layout Two Images g If you want to change the arrangement of the Toolbar uncheck the Lock Toolbar and Menus option Toolbar as well as Menu bar will switch to the mode when it is possible to hide or change the location individual items This way it is possible to set your own user defined interface of the program When you finish arrangements in the Toolbar or menus lock them again by checking the Lock Toolbar and Menus option 172 Tab Miscellaneous Main Program Window In the Slideshow part it is possible to set Delay Between Imag
255. r will change shape and by dragging the mouse while holding the left mouse button you may change the size of the frame as you please By clicking the Center button you may place the frame to the center of the camera s field of view Using the Show Hide button it is possible to hide or display the frame If the frame is hidden the selected region is still being used for measuring of exposition The frame is only not visible so it does not disturb in the live view To define your own exposure region click the Custom button move the cursor to the live view and then by dragging the mouse while holding the left mouse button insert the frame of a desired size 223 Control Panels of Imaging Devices Note If the frame of the exposure region will change its color to red it means that the camera s automatics have reached the limit value of the exposure time The range of exposure times is in the automatic mode smaller than in the manual mode Seco mE Exposure Time if a Manual exposure mode is active you uy 1 215 may using the arrow buttons or by dragging of the slider manually set the desired value of the exposure time To change the exposure time you may also use the and keys on the numerical keyboard or the Page Up and Page Down keys Exp coret F y posure Correction this function enables you to lighten or darken the images when using the Auto exposure mode SoU e e Capture Images in here you can select whe
256. ra and the operating system is established an icon will appear in the right bottom part of the screen 3 Then run the QuickPHOTO INDUSTRIAL 3 0 program 5 Adding the Support of a New Device 5 1 Adding the Support of a New Imaging Device If you want to install the support of a new imaging device into an already installed QuickPHOTO INDUSTRIAL 3 0 program insert the program installation CD to the CD ROM drive and run the installation On page Installation Type Selection select the Update Existing Installation Add Components option and select from the list desired devices whose support would you like to add Continue according to the wizard s guidelines 22 Installation 5 2 Change of the OLYM PUS Camera Type CAMEDIA and SP series If the program QuickPHOTO INDUSTRIAL 3 0 has already been installed with one of the supported OLYMPUS cameras and you want to start using a different OLYMPUS camera of a new type or a different device of the original type from the standard user account it is necessary to obey the following procedure 1 Sign in to the account with the administrator s privileges 2 Connect the new camera switched to the PC control mode for more information see the section called Installation of OLYMPUS Digital Camera on page 14 turn it on and turn the mode dial to the P position Wait until the camera device drivers have been installed 4 Run the tool OLYMPUS SP and CAMEDIA Series Camera Cha
257. rame can be achieved also by double clicking into the frame To return to the standard display mode click the Zoom Jn button again or double click in the zoomed live view Power Supply Power Supply this indicator displays the camera s battery status or AC Adapter the use of the AC power supply 219 Settings Tab Control Panels of Imaging Devices This tab is designed for setting of live view parameters ff Settings Crop Borders Sal Flip Rotate by 180 Cross Grey Scale Crop Borders this slider enables to crop the unwanted borders from the image symmetrically from all sides Use this function in case the darker corners are visible in the live view This phenomenon called vignetting may appear when using smaller magnifications on some types of microscopes The performed setting is interactively displayed in the live view and it will apply also to the acquired images This function does not have any effect on measurement accuracy Frame Rate displays how many images per second Frames Per Second FPS is the camera currently displaying in the live view Orientation Orientation in this part you may set the orientation of the live view mam prin A The performed setting is interactively displayed in the live view and it will apply also to the acquired images i Rotate by 180 Cross displays hides the central cross The dis
258. ring of the perimeter diameter and the circle area counting of objects measuring of angles measuring of the distance between two parallel lines and measuring of the length angle and diameter of an arc Option Phase Analysis activates the Phase Analysis control panel For a more detailed description see Phase Analysis on page 125 Submenu Hardness contains options Brinell Hardness Test and Vickers Hardness Test For more detailed description see Hardness Test on page 133 Options Display Orthogonal Grid and Display Circular Grid is used for displaying hiding of the calibrated grid in the current image For description of work with the grid see Displaying a Grid of Defined Dimensions on page 123 Option Export Measured values to Microsoft Excel Continuously turns on off the function of continuous export of measured values for more detailed description see Exporting Measured Values on page 147 Using the option Units you may set the units used for measuring micrometers or millimeters Option Set Active Calibration enables quick switching between the active calibrations used for measuring if you have created more than one calibration for various microscope 68 Main Program Window imaging device sets Option Calibration Manager opens a dialog box with functions for calibrations managing For more information see Calibration Manager on page 34 For more information about measurements see
259. ror resize automatic white balance white balance defined by point sharpen blur median filter negative grey scale black balance defined by point and functions for brightness adjustment contrast adjustment gamma correction color balance adjustment and histogram adjustment For detailed description of individual functions see Image Editing on page 83 66 Main Program Window Image View Measurements Annotations Imagi Image Information Ctrl I Crop Rotate Clockwise by 90 Rotate Counter Clockwise by 90 u g A e Flip Mirror a Py m Resize Auto White Balance amp White Balance Defined by Point Ctrl T Sharpen Blur Median Filter Negative Grey Scale Black Balance Defined by Point Adjust Brightness Contrast Gamma Adjust Colors ali Adjust Histogram Menu View In the View menu there are the following options Layout Single Image Layout Two Images Layout Four Images These options switch between the modes for displaying of 1 2 or 4 images in the Display Window Option Display Objects in the Current Image will display hide all objects inserted into the current image Option Display Labels with Measured Values in the Current Image will display hide labels with measured values in the current image Option Display Table of Measured Values will display hide Table of Measured Values in the bottom part of the Main Program Window Option Best Fit Zoom will di
260. rosses a measuring tetragon will be interspaced through their centers This measuring tetragon can be in case of need using the green grasp points adjusted so it exactly matches the imprint Vickers Hardness Test dialog box in which the measured sizes of both imprint diagonals and the deviation of their difference from the average length of both diagonals will be displayed From the Mass m drop down menu select the used load in kilograms and into the Time t field enter the corresponding duration time value 135 Main Program Window in seconds In the Vickers Hardness field the calculated material s hardness will be displayed If the value has a red background it means that the difference in sizes of both diagonals is greater than is allowed by EN ISO 4516 2002 standard and the measurement is not valid Note Mass m menu contains only values corresponding with the EN ISO 4516 2002 standard If you want to enter a different value type it in using the keyboard Clicking the OK button will finish the measurement and the measured value will be displayed in the Table of Measured Values Double click on the inserted measuring tetragon will anytime bring about the Vickers Hardness Test dialog box for adjustment of the measuring parameters Vickers Hardness Test Diagonal Length d1 Diagonal Length d2 um Deviation of Diagonals Mass m 0 01 kg Time t 10 gt Es Vickers Hardness HV HY 0 189 S27 _ Ka
261. rrectly Settings can be later changed manually by the option from the menu Edit Options or by repeated running of the wizard from the Edit Configuration Wizard menu Important Full completion of the configuration using the Configuration Wizard is in case of the first run of the QuickPHOTO INDUSTRIAL 3 0 program necessary When you finish the Configuration Wizard the QuickPHOTO INDUSTRIAL 3 0 will run automatically 1 Configuration Wizard This wizard consists of several pages through which you can browse using the Next and Back buttons When configuring proceed according to the wizard s guidelines Imaging Device Selection Second page of the wizard offers the choice of imaging device which will be used for image acquiring You can make your selection among only those devices whose support was Selected during program installation Select the desired imaging device and click the Next button TWAIN Device Selection If you have selected the TWAIN Device option a list of available TWAIN devices will appear Select the device which you want to use for image acquiring and click the Select button 29 Initial Configuration of the Application OLYMPUS Digital Cameras all types This page will appear only in case the support of OLYMPUS CAMEDIA and SP series or OLYMPUS E system SLR cameras has been installed and it is intended to set the device for display of life view from these cameras If you are using a
262. rresponding icon 3 in the Toolbar After the Edit Object Line Color option was activated or after clicking the left part of the l icon select the desired color from the displayed color palette After the Edit Object Line Thickness option was activated or after clicking the right part of the gt E icon select the desired line thickness from the displayed list The icon 77 also serves as an indicator of the currently set line color and thickness Selected line color and thickness will be used for inserting of all new objects Measurement Settings For all newly acquired images and for all opened images which have not yet been saved with the layers kept including some measurements or a scale bar by the QuickPHOTO INDUSTRIAL 3 0 program default settings selected in the Sizes Colors and Measurands tabs in Options of the program Edit Options Measurements Sizes Colors Measurands will be used automatically 116 Main Program Window For the current image displayed in the Display Window these settings can be changed after the first measuring has been done in the Table of Measured Values on the Measurement Settings Image Name tab Changes done on this tab will only relate to the current image For detailed description see Work with the Table of Measured Values on page 139 When saving the image containing some measurements or a scale bar with the layers kept the above mentioned settings are s
263. rted into images automatically after magnification which was used for image acquisition has been assigned You can select whether to insert the Horizontal Scale Bar or the Vertical Scale Bar Note Font size of the scale bar s values is set on the Measurements Sizes Colors tab Options x Eai Scale Bar T Profiles Default Folders ype Image Names amp Saving Scale Bar Type mee Ruler x Save Sound Language Bee Scale Bar Size Do Not Show This Window Annotations Scale Bar Size Small Auto Size X zi 1 Appearance and Layout Miscellaneous Preview 4 Measurements Units 5mm Scale Bar a Sizes Colors Grid Scale Bar Options Measurands Hardness Color of Scale Bar and its Value aa Change Other Settings a 4 Hardware Imaging Devices Background Area Value and Scale Bar X Background Color Change Analog Video Source Remote Trigger Magnification Encoders V Display Scale Bar Value Illuminators Automatic Scale Bar Insertion Horizontal Scale Bar Vertical Scale Bar El Backup Settings a Load Settings 7 Configuration Wizard OK Cancel 176 Main Program Window Tab Sizes Colors On this tab you may set the default properties for all newly acquired images In the Font Size of Scale Bar Value part you may set the font size of the scale bar s value by selecting it from the Font Size drop down menu In the Dim
264. s On the Font Colors tab it is possible to set the lines of measuring objects color of the labels with measured values and the font size of measured values displayed in the live view Scale Bar Tab Options x Font Colors Scale Bar m Units Measurements Scale Bar Type Scale Bar Type Line Segment v Scale Bar Size Scale Bar Size Small Auto Size Scale Bar Options Color of Scale Bar and its Value P Change Background Area Value and Scale Bar Background Color Change V Display Scale Bar Value Font Size 12 v Scale Bar tab contains the options for settings of the calibrated scale bar displayed in the live view In the Scale Bar Type part there are the following options available Bar Line Segment Staple and Stripe In the Scale Bar Size part you may select one out of two automatic sizes Small or Large Option Custom enables to define custom scale bar size After activation of this option a Custom Size field will be available with the option to set the desired scale bar length Color of the scale bar and its value can be selected in the Scale Bar Options part by the Color of Scale Bar and its Value option 203 Control Panels of Imaging Devices Background Area and Background Color options enable you to set the area and color of the scale bar s background If the Display Scale Bar Value option is checked the scale bar s value will also be displayed by the scale
265. s After you choose one of the options a tool for angle measuring will be activated Measure Distance Between 2 Parallel Lines activates a tool for measuring of the distance between two parallel lines Measure Arc activates a tool for measuring of the angle diameter and perimeter of an arc Brinell Hardness Test activates a tool for the Brinell hardness test Vickers Hardness Test activates a tool for the Vickers microhardness test Phase Analysis opens a dialog box of the Phase Analysis function Display Orthogonal Grid displays hides an orthogonal grid of defined dimensions Display Circular Grid displays hides a circular grid of defined dimensions Display Table of Measured Values displays hides a Table of Measured Values F12 73 L O 4 1 T3 74 Main Program Window Export Measured Values to Microsoft Excel Continuously turns on off the function of continuous export of measured values Layout Single Image switches the Display Window to a display mode for one image Layout Two Images switches the Display Window to a display mode for two images next to each other Layout Four Images switches the Display Window to a display mode for four images next to each other Open Imaging Device Control Panel opens a control panel of the active imaging device device chosen in the maging Devices menu An arrow in the right part of the icon is used for a quick choice of the imaging device F4
266. s select them first for more information see Selection of the Measuring Objects for Editing on page 136 To delete selected objects from the image press the Del key activate the Edit Delete Selected Objects option or click the icon To delete all not only the selected objects objects from the image activate the Edit gt Delete All Objects option or click the icon 4 8 Work with the Table of Measured Values Measured values are continuously being displayed in a well arranged table named Table of Measured Values in the bottom part of the Main Program Window The table contains 1 4 tabs with the measured values for individual images displayed in the Display Window number of tabs corresponds with the set layout mode of the Display Window and a tab named Measurements Settings Image Name Measured values of other opened images displayed in the Thumbnail Browser can be displayed in the Table of Measured Values by opening of the desired images in the Display Window Tabs with Names of Images Tabs with names of images contain the measured values for the corresponding images displayed in the Display Window Individual tabs contain the following elements e table of measured values e statistics e toolbar buttons 139 Main Program Window B Table of Measured Values P00001 jpg Measurement Settings P00001 jpg Object Type Measurand Meas Nr Value Description Measurand Length Line Segment Length 1 1
267. s settings can be saved into one of four available profiles and loaded again any time If you want to save the current control panel s settings into a profile click the Save button in the Profiles part From the Save as menu choose the profile you want to save the settings to and enter the name of the profile in the New name field Confirm by clicking the OK button To load a saved profile just select the profile name from the list E Exposure mode if the exposure mode is set to the Auto option exposure Suto acu 3 ll di h Il f h Manuel time s automatically set according to the ilumination of the camera s field of view When using the Auto mode the AEL button Auto Exposure Lock will also be active This button locks the exposure time on the current value If 234 Control Panels of Imaging Devices the Manual option is active value of the exposure time is set by user using the control elements in the Exposure time part Note Auto exposure mode is not available when the camera displays the live view in the highest resolution E areta ime Exposure time if a manual exposure mode J 1 15s is active you may using the arrow buttons or by dragging of the slider manually set the desired value of the exposure time To change the exposure time you may also use the and keys on the numerical keyboard or the Page Up and Page Down keys Se Cross displays hides the central cross D
268. s the camera s memory card contents Erase Settings opens a dialog box with the options for redefining and restoring the profiles Settings Creating a Custom Profile Editing the Existing Profile 1 To create a custom profile set the required camera control panel s parameters on the Camera control tab first Click the ee button A dialog box for setting the profiles will appear Click the button of the profile you want to save the current control panel s parameters to The selected profile will be overwritten If you want to restore the original settings of the Standard Microscope and Macro profiles click the Restore button 212 274 Control Panels of Imaging Devices Settings Profile Save current settings to profile fit 4 f Standard Microscope Macra Custom Default profile settings Live image Settings Control Panels of Imaging Devices 9 Analog Video Source Analog video source i Capturing Image settings Device settings Ez Timestamp 22 Profiles a E Capture Full screen The control panel allows acquiring of images from analog video sources The images can be acquired e g from analog cameras video endoscopic systems ultrasonographs or DVD players Capturing Tab m Capture captures an image and transfers it to the computer Capture screen Using this type of display you can control the program using the Capture and In window buttons
269. s window which enables you to choose the location names and types of files being saved Window Save As Window Save As replaces the standard Windows dialog box for saving files into a chosen location This window contains an expanded functionality and it enables saving of more images at the same time This window consists of several parts e Thumbnails of Images for Saving e Location e File Name and Type e Additional File Name Tags e Numbering e Flattening Layers e Information 103 Main Program Window Thumbnails of Images for Saving Location Q Save As x Number of Images to Save 5 Selected 7 ee to 3 sample jpg r M WON 2776 x 2074 PEG Quality 95 Kani F Me sample2 b sample3 fem sample jpg 2560 x 1920 JPEG Quality 95 E samp E sample3 jpg 3123 x 2339 JPEG Quality 95 a 2 sample4 jpg 2776 x 2102 JPEG Quality 95 File Name and Type Additional File Name Tags Selected Images Numbering All Images s Numbering Numbering Flattening Layers by A Images with yellow exclamation mark already exist in the current folder and will be overwritten r Some images are going to be saved as BMP bmp or PNG png type These types do not suppo l nfo rm at lon Additional File Name Tags Part Thumbnails of Images for Saving This part contains thumbnails of all images to be saved After the Save
270. sabled or the control panel is terminated Timestamp Tab It is possible to automatically insert into the acquired images date and time of their acquisition the so called timestamp The timestamp will after acquisition of the image become a solid part of the image and it is not possible to remove it from the image other way than by cropping it 212 Control Panels of Imaging Devices EE Timestamp Timestamp Disabled o Enabled Vv Date V Time Seconds Abc Timestamp this option turns the Timestamp function on off Disabled Enabled Insert by checking of individual options it is possible to Date define which components of the timestamp will be inserted Time into the acquired images Seconds Orientation this field serves as an indicator of the set orientation of the timestamp If you double click into this Abc field a Timestamp Settings dialog box will open Options if you click this button a dialog box for setting of timestamp parameters will open Options Timestamp Settings If you click the Options button or if you double click into the Orientation field a dialog box for setting of the timestamp will open 213 Control Panels of Imaging Devices 3 Timestamp Settings EE v0 of Image Width Transparent Top teft Bottom Left 07 09 2012 16 29 27 o Cancel Format using the options in this part it is pos
271. setting is such the center of the field of view is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Automatic white balance button 4 On the Capture tab set the desired image size 5 Switch to the Shading correction tab and select the Enabled option In the umination part select the Transmitted option Then in the Calibration mask part click the Capture mask button Preview of the mask will be displayed in the control panel 6 Place the specimen back in and set the desired exposure time 7 In the Correction level part click the Preview in live image button According to the need you may using the Correction level slider adjust the correction Reflected light 1 Set the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Place a white sheet under the microscope for example a sheet of white paper Do not focus the microscope and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view 263 Control Panels of Imaging Devices is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black Click the Automatic white balance button On the Capture tab set the desired image size 5 Switch to the Shading correction tab and select the Enabled option In t
272. settings Using the slider you may set the level of correction interactively Using the Shading Correction Function Important Shading correction gives good results only if all the settings of the microscope and the camera are the same while acquiring images as when capturing a calibration mask If you change the conditions change of magnification change of illumination change of image size and so on it is necessary to acquire the calibration mask again To use the Shading Correction function it is necessary to create a calibration image the so called mask first Proceed as follows Transmitted Light 1 Focus the microscope using the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Remove the specimen and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Auto WB button automatic white balance on the Zmage tab 4 On the mage tab set the desired image resolution 5 Switch to the Shading Correction tab and select the Enabled option In the umination Type part select the Transmitted option Then in the Calibration Mask part click the Capture Mask button Preview of the mask will be displayed in the control panel 6 Place the speci
273. sible to set the appearance of the timestamp Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text TAD Preview in this field preview of the timestamp is 07 09 2012 16 29 39 displayed the way it will be inserted into the acquired images except for the orientation option Profiles Tab All settings of the control panel except for the settings of the Shading Correction function can be saved into one of the four profiles and at any time loaded again 214 Control Panels of Imaging Devices a Profiles Profiles Default 1 i Default 2 Hi Default 3 Default 4 bell Save to Profile W Default Profile Profiles buttons in the Profiles part are used for loading of the saved profiles fsavetoprofle Save to Profile if you click this button a dialog box will be displayed for saving of the current control panel s settings into one of the profiles B pefauitProfie Default Profile if you click this button all elements of the control panel will be set to the default values Saved profiles will not be affected in any way by this action Saving of the Control Panel s Settings into the Profile To save the settings into the profile proceed as follows 1 Set the desired parameters of the control panel
274. soseceseessssssoseseeessesosssssseesssssssesssesseseses r R a A lt Back Next gt Cancel 4 Control panel of the active imaging device with a live view of the stage micrometer opens Place the micrometer horizontally to the center of the imaging device s field of view and focus the microscope Click the Capture button Next page of the guide 3 Calibration will open with the image of the stage micrometer If the quality of the calibration image is not satisfactory it is possible to open the imaging device control panel again to acquire a new calibration image using the icon In the displayed image mark the highest possible number of the stage micrometer segments with a line segment by dragging the mouse while holding the left button or by clicking the left mouse button on the beginning and end point Insertion of a precisely horizontal or vertical line segment can be done by holding the Shift key while inserting the line segment To achieve the highest possible accuracy it is good to enlarge the image using icons in the upper left part of the dialog box or by using the mouse wheel before inserting the calibration line segment Lead the line segment from the left edge of the initial guideline to the left edge of the ending guideline of the calibration scale Incorrect insertion of the line segment can be corrected by inserting a new calibration line segment Calculate the real length of the marked part of
275. speed can be locked by clicking the AEL Auto Exposure Lock button The Aperture priority option the aperture value can be selected from the drop down menu and the shutter speed will be calculated automatically This option is available only when the lens is attached to the camera body The Shutter 243 Control Panels of Imaging Devices priority option the shutter speed can be selected and the aperture will be automatically calculated Metering mode Metering mode here you can select one of several exposure metering modes See the user s guide of the camera for more details Center spot Spot Shadow Resolution Resolution for some quality options the image resolution can be selected from this drop down menu 256041920 10248768 B40 480 Sequence Sequence here you can set the number of images to be captured in the sequence a SEn Sensitivity sets sensitivity of the sensor The options conform to the sensitivity of traditional photo materials in the ISO scale When Auto option is selected the camera changes the sensitivity automatically depending on the current illumination conditions Shutter speed Shutter speed when capturing images in the Manual or Shutter priority mode 18 here you can set the required shutter speed Aperture Aperture when capturing images with the lens attached in the Manual or Aperture priority mode here you can set the required aperture value White balance this option
276. splay the current image in such a size so it would fit into the Display Window Options Zoom In and Zoom Out will enlarge reduce display of the current image by one step Option Zoom Actual Size 100 will display the current image zoomed in by 100 so that one image pixel corresponds with one pixel of the monitor Option Start Slideshow will activate full screen view of all the opened images in case only one image is selected or images selected in the Thumbnail Browser Display of all or only of selected images will take place successively in a time interval which can be chosen in the program options 67 Main Program Window Option Display in Full Screen Mode will display an image images opened in the Display Window full screen You may return to the standard mode by pressing any key View Measurements Annotations Imaging Devices Modules Re Layout Single Image Bi Layout Two Images Layout Four Images Display Objects in the Current Image Display Labels with Measured Values in the Current Image Display Table of Measured Values F12 Best Fit Zoom Zoom In v visis B Zoom Out _ _ Zoom Actual Size 100 Start Slideshow Alt S Display in Full Screen Mode Ctrl F Menu Measurements Menu Measurements contains the following tools for measuring calibrated scale bar insertion measuring of the line segment and polyline length measuring of the perimeter and area of an ellipse rectangle and polygon measu
277. ssible to delete a whole layer with inserted objects or it is possible to merge this layer with the image 1 Description of the Graphical User Interface Main Program Window is divided into several parts e Menu Bar contains options with individual functions e Toolbar contains tool icons e Thumbnail Browser displays all opened images e Navigator is used for orientation in the enlarged image e Display Window is used for image editing e Table of Measured Values contains results of measurements e Status Bar contains information about the current program status 62 Main Program Window Display Window Q QuickPHOTO INDUSTRIAL 3 0 samplel jpg sox Menu Bar gt File Edit Image View Measurements Annotations Imaging Devices Modules Reports Window Help SH Se o Ba t objective 0 5 Zoom 0 8 lt 2 00 omge oe PP P 11 0 Juf Em oA lB 33 Thumbnail Browser R Selected Q Image Count 4 1 Toolbar z Y S 5 sample 1 jpg 1194x 973 gt a Ea iJ f A SO z N i x 3 eG Sol Gui zi J gt 800 x 600 E Thumbnail Browser 396 HES 10 3000 z z sample3 jpg ate J 698 x 563 a 3 H a sample4 jpg Sa 2560 x 1920 S 2 i ay 0 5mm mom gt es y NN sample1 jpg sample2 jpg sample4 jpq sample3 jpg Measurement Settings sample1 jpa h ACSA O Object Type Measurand Meas
278. ssions for writing must be opened in it To make the 147 Main Program Window opened document accessible for continuous exporting the Microsoft Excel program must be running with the same privileges as the QuickPHOTO INDUSTRIAL 3 0 program for example both programs with the standard user s privileges or both with administrator s privileges If each of the programs will be running with different privileges a warning message will be displayed and this function will not be activated This function can be activated by the Measurements Export Measured values to Microsof Excel Continuously option or by clicking the icon in the Toolbar When the function is successfully activated the 1 icon in the Toolbar will be color coded and pressed and in the Measurements menu the Export Measured values to Microsof Excel Continuously option will be checked If more workbooks are opened in the Microsoft Excel program or multiple instances of the Microsoft Excel program are running a dialog box with a list of available workbooks will be displayed during activation of this function Continuous exporting function will be activated after selection of one of the workbooks from the list To deactivate this function use the Measurements Export Measured values to Microsoft Excel option or click the amp icon in the Toolbar again 5 Reports Program QuickPHOTO INDUSTRIAL 3 0 enables export of images with measured values to reports
279. stallation of a device for displaying of life view e Installation of OLYMPUS digital camera These steps are described in the following chapters Installation of a Device for Displaying of Life View Program QuickPHOTO INDUSTRIAL 3 0 can be for the use with OLYMPUS digital cameras E system CAMEDIA and SP series used with one of these devices for displaying of life view on a PC monitor 1 External USB 2 0 device AV USB 2 0 PRO 2 Internal PCI card Leadtek WinFast VC100 XP 11 Installation Important For correct displaying of life view it is necessary to have set in the Microsoft Windows operating system displaying of 24 or 32bit color depth Installation of an External Device AV USB 2 0 PRO l Connect the AV USB 2 0 PRO device to the USB 2 0 port of your turned on computer 2 Ifa Found New Hardware wizard opens up terminate it Installation of the AV USB 2 0 PRO device drivers will take place during installation of the QuickPHOTO INDUSTRIAL 3 0 program Installation of the PCI Card Leadtek WinFast VC100 XP 12 Important If you are installing the Leadtek WinFast VC100 XP card into your computer in which is or has been installed the ATI TV Wonder card it is necessary for proper functionality to remove the ATI card and insert the Leadtek card into another PCI slot In case you do not follow this procedure it is possible that your computer will not work properly Important Leadtek WinFast V
280. sts of a toolbar tabs with control elements and the live view 189 Note Control Panels of Imaging Devices A performance test will take place automatically during the first run of the control panel Several test images will be acquired during the test which can be deleted right after the test Toolbar Toolbar which is placed above the live view contains the following buttons Capture miim Full Screen Region WE Region BB 190 Capture Ctrl Enter after clicking this button an image will be acquired and automatically transferred to the computer If the camera is not connected the button will change to Connect camera aol button Full Screen Ctrl F this button is used for displaying of live view full screen Using this type of display you can control the program using the Capture Ctrl Enter button which is placed in the left upper corner of the screen and the Exit Full Screen Mode button placed in the right upper part of the screen Automatic White Balance this button activates the function for automatic white balance It is best to do this balancing when the camera is capturing a white area White Balance on a Defined Region clicking on this button and successive dragging of the mouse in the live view while holding the left mouse button will define a region which will be used as reference for the white balance Black Balance on a Defined Region clicking on this button a
281. sured in the image images displayed in the Display Window number of these tabs corresponds with the set layout of the Display Window and a tab with the name Measurement Settings Current Image Name For a more detailed description see Work with the Table of Measured Values on page 139 E Table of Measured Values P00001 jpg Measurement Settings P00001 jpg Measurand i ipti 4 Measurand Length Length Number of Measurements 3 Length 2 Minimal Value 197 Line Segment Length Maximal Value 200 Ellipse Perimeter Average Value Perimeter Standard Deviation Perimeter L BE amp g Undock Dimensions Perimeters Areas Angles Objects Phases 79 Main Program Window 1 7 Status Bar Status bar placed in the bottom part of the Main Program Window displays the following information e size of the current image in pixels and if the current image has an assigned calibration real size of the image in mm or um if the magnification encoder is being used magnification of the current objective or possibly the zoom currently selected imaging device if you have set the automatic saving of images to a folder there will be an icon in the right corner of the status bar If you move the mouse over this icon a hint will be displayed with currently set folder for automatic saving of images 2 Acquiring of New Images To acquire new images proceed according to the following steps 1 Select the im
282. t The green black frame will be displayed in the preview This frame defines the area which will be scanned in resolution set by the Jmage Resolution DPI option after clicking the Scan Image button The size of the frame can be modified by mouse using the green grasp points in its corners The default frame s size can be set by double clicking into the preview area The next double click will set the last used frame size 241 Control Panels of Imaging Devices 6 OLYMPUS E system Digital SLR Cameras i OLYMPUS E system SLR camera E 410 Gi Capture Macro Colors BW Live view 0 Shading correction 2 Timestamp B2 profiles A Quality Resolution Sensitivity White balance 5Q v 1280x960 v auto v Auto E Program ai Shutter speed Anti Shock time Metering mode Sequence Aperture Exposure compensation Center Spot vi 1 The control panel is intended for displaying a live image controlling the camera functions and capturing images from OLYMPUS E system SLR cameras The controls are located in seven tabs Important Control panel of OLYMPUS E 330 SLR camera is not equipped with several functions described below The E 330 SLR camera can be controlled only from the accounts with administrator s privileges in Microsoft Windows XP operating system This camera can not be controlled from the Microsoft Windows Vista 7 8 operating systems 242 Control Panels of Imaging Devices
283. t mm Height will be calculated automatically to constrain proportions of the image lt Back Next gt Cancel In the next step all tables found in the pattern document will gradually be evaluated on the Processing Table from page in a similar way The wizard will give you a chance to decide with every found table what shall be the purpose of each currently displayed table in the template If the table is supposed to be a fixed part of the report check the first option No this table is a part of the report and should be retained If the displayed table serves as a mark for determination of the place for inserting of the table of measured values from the QuickPHOTO INDUSTRIAL 3 0 program check the option Yes link this table with measured values from image number and from the drop down menu select an image number with which the table should be linked In the bottom part of this page it is possible to select the measurands and columns which the table should contain In the default state all measurands and columns are checked If you do not want to have some of the measurands or columns in the table uncheck them In the right bottom part a preview of the final table is displayed If you want the report to contain the measured values of the Brinell or Vickers hardness tests check the Hardness option But in this case other measurands cannot be displayed in the table Main Program Window Continue by cli
284. t The Sensitivity ISO drop down menu sets the sensitivity of the sensor The Exposure Time drop down menu is enabled only when the manual exposure mode is set You can set the required exposure time by this menu White Balance the white balance can be done in two ways If you click the Auto button the white balance will be performed automatically continuously by the camera Use this function in case you want the digital image to have the same color balancing as the image visible in the eyepieces If you click the One Point button click the left mouse button to the desired point of the live view which will define the reference white color The white balancing will be performed according to the selected reference color Exposure Region in case of automatic exposure mode is set the area defined by the rectangular region displayed in the live view is used for exposure measurement The region s size cannot be changed however the frame can be moved by a mouse Using the Display Hide button it is possible to hide or display the frame If the frame is hidden the selected region 1s still being used for measuring of exposition The frame is only not visible in order not to disturb in the live view By clicking the Center button you may place the frame to the center of the camera s field of view The Zoom In button zooms in the area defined by the frame This functions facilitates the microscope focusing The zooming in of the area defined by the f
285. t pi fe Reflected or T ransmitted option The correction method is different for each illumination type Calibration mask 3648x2736 Calibration mask if you click the Capture mask button an image which will be used as a calibration mask for shading correction function will be acquired In the field apture mask j f next to the button will be displayed the preview of the captured mask and information about its size Correction level Correction level using this slider you may set the J 255 strength with which will be the shading correction applied Using the Shading Correction Function Important Shading correction function gives good results only if all the settings of the microscope and the camera are the same while acquiring images as when capturing a calibration mask If you change the conditions change of magnification change of illumination change of image size and so on it is necessary to acquire the calibration mask again To use the Shading correction function it is necessary to create a calibration image the so called mask first Proceed as follows Transmitted Light 1 Focus the microscope using the desired microscope s magnification 2 Remove the specimen and on the Capture tab set the desired image Quality Resolution and White balance 3 Switch to the Shading correction tab and select Enabled option In the umination part select the Transmitted option Then click the Capture mask button A t
286. t of the image and it is not possible to remove it from the image other way than by cropping it Ea Timestamp Timestamp Insert Orientation Preview Position top left Disabled Date Oem tine Abc 12 10 2012 1 58 19 PM Settings V Seconds et Timestamp this option turns the Timestamp function on off Disabled Enabled 251 Control Panels of Imaging Devices Settings Settings if you click this button a dialog box for setting of timestamp parameters will open Insert Insert by checking of individual options it is possible to define which ee components of the timestamp will be inserted into the acquired images Ime Seconds Orientation Orientation this field serves as an indicator of the set orientation of the timestamp If you double click into this field a Timestamp settings dialog Abc box will open Preview Position top left Preview in this field preview of the timestamp is displayed the way it will be inserted into the acquired images except for the orientation option The text size is the same as will be displayed in the acquired images in the 100 1 1 display size 12 10 2012 1 58 19 PM Position the indicator shows the position where the timestamp will be inserted in the acquired images Timestamp Settings After clicking the Settings button or double click to the Orientation field the Timestamp settings dialog box will open 252 Control Pa
287. t the camera s speed according to the computer s performance Shading Correction Tab This tab contains control elements for the shading correction function This function may be used to remove deficiencies in the image caused by inhomogeneous illumination removal of the darker corners of the image or by dust on the sensor Eo EE OERTOEIE b 4 Shading Correction Shading Correction Calibration Mask 1280x960 Correction Level Disabled 2 255 Enabled y Illumination Type Pc apture Mask i Transmitted piece ies Shading Correction this option turns the Shading Correction function Sale E Enabled on off O e AE Illumination Type in dependence on the type of the used illumination Reflected Transmkted select Reflected or Transmitted option The correction method is different for each illumination type Seren Nachle Calibration Mask if you click the Capture Mask button an _ image which will be used as a calibration mask for shading oe correction function will be acquired In the field next to the button will be displayed the preview of the captured mask and information about its size 22 Control Panels of Imaging Devices Correction Level Correction Level using this slider you may set the Yl 255 strength with which will be the shading correction applied If you click the Preview in Live View button you will see the effect of the shading correction function reflecting the current
288. talic 4 or Underline buttons Font color can be chosen from the color palette which will show after clicking the Text Color button Font size and type can be selected from the A a drop down menus To align a multiline text 93 Main Program Window use the following buttons Align Multiline Text Left Center Multiline Text and Align Multiline Text Right 2 Orientation of the text can be chosen in the Orientation part by clicking the corresponding button with the Abc text on it Orientation Abc Abc 30 IgV Background of the text annotation can be set in the Background Color and Background Area parts If you in the Background Area part check the Transparent option text annotation will be inserted into the image with a see through background If you check the Custom Color option it will be possible after clicking the button to choose the annotation s background color from the displayed color palette After checking the Custom Color option the Background Area part with two choices will be made available Option Rectangle will fill in the whole rectangle area defined by the text annotation with the set background color Option Behind Text Only will fill in only the area behind text with the set background color To insert a text annotation click the OK button Note Text writing and all above mentioned editing are continuously displayed directly in the image Parameters of the text annotation
289. tarts to rise Continue rotating the focus knob until the blue strip starts to decline Red guideline will remain on the position of the maximum reached focus value Gently rotate the focus knob in the opposite direction now achieve that the blue strip reaches as closely as possible to the red guideline in this position image of the selected area will be as sharp as possible You can use numerical values in a similar way for focusing Note Focus level is measured relatively That means the indicator shows whether the focus value is rising or declining towards the default value which is calculated after the frame is positioned If the focus value exceeds the indicator s range it is initialized again to about three quarters of the range That does not mean that the focus value would decline rapidly If you continue rotating the knob in the same direction and the strip rises again 1t means that the focus level continues to rise Reset button initializes the indicator again into the default state That means the maximum reached value of focus level is set along with the current value approximately to three quarters of the range Using the Show Hide button you can hide or display the frame of the focus region Note If you relocate or change the size of the focus region automatic reset of the focus indicator will take place see Reset button 229 Control Panels of Imaging Devices Options Tab This tab contains control
290. ted Objects in a Separate Layer Merge Objects with an Image Flatten Layers Quality 95 High Small File Large File File Size Not Calculated Calculate In the top part of the window there is a list JPEG and TIFF Images to Be Saved which contains names of images which will be involved in the settings The same settings will be used for all images on this list In the middle part of the window there is a Flattening Layers option available for more information see Information about Work with Layers on page 97 If there is at least one JPEG type image in the list there is a JPEG Quality section in the bottom part of the window Controls in this section enable setting of quality for the JPEG type images This option has no influence on other types of images Calculate and Preview buttons are available only if there is only one JPEG type image on the list of images to be saved Calculate button calculates the resulting image size at the set JPEG quality Preview button displays a window with the image preview as it will look at the chosen JPEG quality 102 Main Program Window Option Save As Option File Save As saves images selected in the Thumbnail Browser using the Save As window which enables to choose the location names and types of files being saved Option Save All As Option File Save All As saves all images displayed in the Thumbnail Browser using the Save A
291. text file for import to other spreadsheet programs values are separated with a tab symbol Important For exporting of measured values into the xls format it is necessary to have on your computer installed the Microsoft Excel program To export measured values from the current image displayed in the Display Window click tx the Export Values tyauss button in the bottom part of the Table of Measured Values or activate the File Export Measured Values from Selected Image option For exporting of measured values from the image you may also use the icon in the controls of the thumbnail in the Thumbnail Browser 144 Main Program Window For exporting of the measured values from all opened images activate the File Export Measured Values from All Images option Export Measured Values dialog box which replaces the standard Windows dialog box for saving of files will open This dialog box contains expanded functionality and enables saving of more files at the same time This dialog box is very similar to the dialog box for saving of images and it consists of the following parts e Files for Export e Location e File Name and Type e Additional File Name Tags e Numbering e Information Files for Export Location Q Export Measured Values File Name and Type Additional File Name Tags Selected Files oe Er Numbering L ormati Files with a yellow exclam
292. the Capture mask button an image which will be used as a calibration mask for shading correction function will be acquired In the field next to the button will be displayed the preview of the captured mask and information about its size Correction level Correction level using this slider you may set the strength with which will be the shading correction applied If you J click the Preview in Live View button you will see the effect ad of the shading correction function reflecting the current settings Using the slider you may set the level of correction 262 interactively Control Panels of Imaging Devices Using the Shading Correction Function Important Shading correction gives good results only if all the settings of the microscope and the camera are the same while acquiring images as when capturing a calibration mask If you change the conditions change of magnification change of illumination change of image size and so on it is necessary to acquire the calibration mask again To use the Shading correction function it is necessary to create a calibration image the so called mask first Proceed as follows Transmitted Light t Focus the microscope using the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Remove the specimen and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal
293. the OLYMPUS CAMEDIA and SP Sehes Dizital C aMMeras sets castdct ota aaa a ET 22 5 Adding the Support of a New DEVICE ects cass cipctacsadeccaetaeedecstudecaentaetersaes 22 5 1 Adding the Support of a New Imaging Device c cc cc eee ce ee ee ee ee sees 22 5 2 Change of the OLYMPUS Camera Type CAMEDIA and SP series 23 5 3 Adding the Support of Magnification Encoder or a PC Controlled Oa O saccharin E E A A TE 23 Initial Configuration of the Application nsonnssnnnsenssennsseessressrrsseresseesrersseesse 25 UConneuratnon Nizar d ei E lea clue E E Seca eeea ese eee 25 Calibration of the Program for Measurements ccccccec ese eeeeceeeeeeeeseneneeaens 33 1 Opening Information about Calibrations 0 0 cece cece ec ec eee neee eee eeeeeenenes 33 DAC All DYAUIOMs Maina Ber Ae cece deh aaar N EEE EEEE NES 34 A reatnT a New CaliDration nean oona E a ace eiaes 35 3 1 Creating a New Standard Calibration Manual Magnification Assignment MOJE Cacti dee rahe accel utente aa eke ea eek caine E achat tee hated ta tt 35 QuickPHOTO INDUSTRIAL 3 0 3 2 Creating a New Calibration Using the Magnification Encoder for Upright and Inverted WICIOSCODES scecas23c anni ecT sin e S S EA meade nanca ela ATRAEN 46 3 3 Creating a New Calibration Using the Magnification Encoder for Ster eOMMNCLOSCOPES ehseiccccsaiadncesec a a A A E 52 4 Setting an Active Calibration se i uissih ne raa e eomtieb
294. the Shading Correction function until the function is disabled or the control panel is terminated Timestamp Tab It is possible to automatically insert into the acquired images date and time of their acquisition the so called timestamp The timestamp will after acquisition of the image become a solid part of the image and it is not possible to remove it from the image other way than by cropping it Timestamp Insert Orientation Preview Position Top Left Disabled Date ee ae 16 10 2012 11 21 30 Timestamp Timestamp this option turns the Timestamp function on off Disabled Enabled 229 Control Panels of Imaging Devices Options if you click this button a dialog box for setting of timestamp parameters will open Insert Insert by checking of individual options it is possible to define which me components of the timestamp will be inserted into the acquired images Ime Seconds Orientation Orientation this field serves as an indicator of the set orientation of the timestamp If you double click into this field a Timestamp Settings dialog Abc box will open Preview Position Top Left Preview in this field preview of the timestamp 16 10 2012 11 21 30 is displayed the way it will be inserted into the acquired images except for the orientation option The text size is the same as will be displayed in the acquired images in the 100 1 1 display size Position the indicator sho
295. the mouse click and you click the Table of Magnifications button a Table of Magnifications Calibration Calibration Name dialog box containing calibration values for each magnification of the microscope imaging device set opens It is possible to lock all the calibrations from unauthorized changes with password protection For locking click the Lock Calibration button and enter the password After locking it will not be possible to make any changes in calibrations until calibrations are unlocked again by clicking the Unlock Calibration button and entering the password which was used for locking It is possible to export all the created calibrations into a backup file format xml by clicking the Backup Calibrations button If calibrations need to be restored it is possible to load the saved calibrations from the backup file by clicking the Load Calibrations button and choosing the particular file containing calibration backup In the bottom left part of the Calibration Manager dialog box there is a date and time of the last change made 3 Creating a New Calibration 3 1 Creating a New Standard Calibration Manual Magnification Assignment Mode Before creating a new calibration put the stage micrometer under the microscope set the lowest magnification and focus the microscope The process of creating a new calibration consists of two main steps e adding a new standard calibration e standard calibration of the individual magn
296. their original locations If we are speaking about newly acquired images a dialog box Save As will be displayed and you may choose the location name and type of the files being saved Options Save As and Save All As will open the Save As dialog box which enables you to choose the location name and type of the files being saved For more information about image saving see Image Saving on page 97 Options Export Measured Values from Selected Images and Export Measured Values from All Images will export the measured values from the images selected in the Thumbnail Browser respectively from all opened images Measured values can be exported into a file of the Microsoft Excel xls format or of the text file txt format For more information about export of the measured values see Exporting Values from the Table of Measured Values on page 144 Options Close Selected Images and Close All Images will close images selected in the Thumbnail Browser respectively all opened images Option Open Imaging Device Control Panel will open a separate window for acquiring images using the imaging device For more information about work with control panels of the imaging devices see Control Panels of Imaging Devices on page 189 Option Print will open a dialog box for image printing For more information about print see Image Printing on page 108 Option Exit will end the program 64 Main Program Wind
297. ther Live view Resolution 1280 x 960 3 Full Resolution 2560 x 1920 the images should be captured in the resolution of the live view or in full camera s resolution This option is active only when using certain types of IDS uEye cameras me ee Color Mode you can switch between Color or Grey Scale modes olor O Grey Scale Cross Cross displays hides the central cross Display Central Cross Peed Focus Region this function makes it easier to focus the selected area of the image To use this function proceed as follows Display Hide 1 Using the focus knob roughly focus the microscope Click the Custom button in the Focus Region part Move the cursor into the live view and insert the frame in the live 224 Control Panels of Imaging Devices view by dragging the mouse while holding the left mouse button to a place where you want to do the focusing 2 Focus indicator will be displayed next to the frame Blue strip represents the current focus value and the red guideline maximum reached focus value Both values are at the same level at the beginning Both values are also displayed in a numerical form in the title of the frame in the current value maximal reached value form 3 Ifyou start rotating the microscope focus knob now the blue strip will start to decline respectively rise according to the fact whether the focus level in the framed area will decrease or rise Select such a direction so the strip s
298. these packages installed in your system we strongly recommend to uninstall such packages If you need to install some codec use the particular codec installer instead of installing the whole codec pack After signing in with the administrator s privileges proceed as follows 1 Insert the installation CD and run the installation of the QuickPHOTO INDUSTRIAL 3 0 program by clicking the Install QuickPHOTO INDUSTRIAL 3 0 button from the home screen of the installation CD If the home screen does not show automatically run from the installation CD program autorun exe 2 Setup Wizard of the QuickPHOTO INDUSTRIAL 3 0 program will open Continue according to the wizard s guidelines On page Select Components Imaging Devices select the support of the desired imaging devices If you want to use an imaging device 17 Installation controlled via the TWAIN interface select the Imaging Devices Controlled via TWAIN Interface option Continue according to the wizard s guidelines If you want to use some of the magnification encoders select the corresponding control unit s on page Select Additional Components Magnification Encoders Continue according to the wizard s guidelines If you want to use some of the computer controlled illuminators select the corresponding control unit s on page Select Additional Components PC Controlled Illuminators Continue according to the wizard s guidelines On page Select Additional Tasks in the D
299. tivated Objective 10 Zoom jm ZQEUC Y h A T2ASUE HO Mode of Assigning Magnifications to Images If the same microscope magnification is being used frequently manual assigning of the used magnification to each new image might be uncomfortable For such cases offers the QuickPHOTO INDUSTRIAL 3 0 program in the Options dialog box in section Measurements on the Other Setting tab Edit Options Measurements Other Setting the option Mode of Assigning Magnifications to Images This option offers two possibilities 115 Main Program Window e Manual Magnification Assignment to Each Image if this option is active default option it is necessary to manually set magnification from the Objective or eventually Zoom menu to each image intended for measurements e Automatically Assign the Last Used Magnification to a New Image if you activate this option to each new image will automatically be assigned the last magnification assigned to some image Note If you are using one of the magnification encoders values of magnifications in the Objective or eventually Zoom menu will be automatically filled in when the image is acquired and values completed in such a way cannot be changed manually Setting the Line Color and Thickness of the Measuring Objects To set the line color and thickness of the measuring objects use the Edit Object Line Color and Edit gt Object Line Thickness options or co
300. uality 95 High Small File Large File File Size Not Calculated Calculate fe Note Default value of the JPEG quality is in the QuickPHOTO INDUSTRIAL 3 0 program set at the value 95 You can set the default value by the Default JPEG Quality parameter in the Edit Options General Image Names amp Saving program setting Note JPEG type file is not very suitable for saving of images with measured phases We recommend to use the TIFF file type for such images 99 Main Program Window e Information about TIFF File Type TIFF file type Tag Image File Format suffix tif represents a commonly used option for image saving using a lossless compression There is no decline in quality of the saved file which makes this type of file very suitable for the need of high quality print Disadvantage of this file type is its large data size This file type does not allow the user to set the quality TIFF type enables to save not only the image information but also additional data Thanks to this it is possible to use the TIFF type to save images in the QuickPHOTO INDUSTRIAL 3 0 program with the layers kept as they are e Information about PNG File Type PNG file type Portable Network Graphics suffix png also uses the lossless compression for saving image data PNG type produces unlike the TIFF type significantly smaller files but it does not allow in the QuickPHOTO INDUSTRIAL 3 0 program to save images with the
301. uick and easy settings of the camera functions Each profile may contain settings for all the functions of the Camera control tab The Standard Microscope and Macro profiles are predefined and may be redefined at any time The Custom profile is intended for saving of user settings fit Standard preset profile for standard digital photography e g using tripod standad SHQ resolution is set as well as automatic exposure mode automatic white balance automatic flash and focusing and ESP exposure mode Microscope the following parameters of the digital camera will be set for Microscope acquiring images from microscopes maximum zoom flash switched off manual focusing mode set to infinity value The other parameters are identical to the Standard profile x Macro this is a predefined profile for macro imaging The camera will be set Maco to macro focusing mode with the spot exposure metering mode f Custom this profile is prepared for storing your personal settings Custom Note Upon activation ofone ofthe profiles a test image will be automatically captured to set the camera correctly It is then deleted immediately 212 Control Panels of Imaging Devices Toolbar Other Functions ce Connect this button connects the camera when disconnected Connect m Live image turns on off the live view Live image a Refresh use this button for refreshing the live view e g in case of over Retesh illumination Erase erase
302. using QuickPHOTO INDUSTRIAL 3 0 program with multiple microscope imaging device sets and you have created in the Calibration Manager more than one calibration it is important to set the corresponding calibration as active when you change the active imaging device This particular calibration will be used for newly acquired images Setting an active calibration can be done three different ways e In the Calibration Manager choose the requested calibration from the list and click the Set as Active button Next to the name of the active calibration will appear a sign Active e From the menu bar using the Measurements Set Active Calibration option Calibration will be set as active if you choose it from the displayed list This listed calibration will be marked by the check mark Insert Horizontal Scale Bar f Insert Vertical Scale Bar Va Line Segment Elipse Rectangle Circle gt Polygon gt Polyline et Count Objects gt Angle gt Distance Between 2 Parallel Lines Arc LA Phase Analysis Hardness gt Display Orthogonal Grid Display Circular Grid 3 Export Measured Values to Microsoft Excel Continuously Units gt Set Active Calibration CCD Camera Stereomicroscope Calibration Manager Ctrl Alt C CCD Camera Upright Microscope e If you activate another imaging device in the Jmaging Devices menu dialog box Select Active Calibration for Imaging Devices Name will be
303. ved or measured values are exported into a file If the description of an image is filled in there will be an indicator Image contains description displayed by the thumbnail Selection of one image in the Thumbnail Browser can be done by one click of the left mouse button on the corresponding thumbnail Continuous selection of several images in the Thumbnail Browser is possible by clicking the mouse on the first thumbnail and then on the very last one of your selection while holding the Shift key Discontinuous selection of images in the Thumbnail Browser can be done by selection of desired thumbnails with the left mouse button while holding the Ctrl key Selection of all images at the same time can be done by the Ctrl A key shortcut Canceling of the selection of more thumbnails can be done by clicking the left mouse button on a random thumbnail Order of thumbnails may be changed by dragging them with a mouse drag and drop To gain a larger work area you may hide slip in to the left the Thumbnail Browser by clicking the kd button in its right upper corner This button then will change to button This way the Thumbnail Browser will free up and if you move the mouse cursor outside of the Thumbnail Browser it will be slipped in to the left In this case you may work with Thumbnail Browser by moving the mouse onto the vertically oriented Thumbnail Browser tab on the left side of the Main Program Window Thumbnail Browser will be
304. versions of the program 1s compatible with the Microsoft Windows 7 8 operating systems In these operating systems use the AV USB 2 0 Pro device Note When using the AV USB 2 0 Pro device with the control panel of OLYMPUS CAMEDIA and SP series digital cameras on the computer where the Grand AV USB 2 0 device has been installed white USB device delivered with the previous versions of the program an error message may be displayed because of the cresprop ax file In this case use the AV USB 2 0 Driver Cleaner tool for removing of the Grand AV USB 2 0 device drivers from the operating system This tool can be found on the QuickPHOTO INDUSTRIAL 3 0 installation CD in the Utilities folder In the control panel of the OLYMPUS digital camera it is not possible to see the live view it is black and white or it has improper hue 1 Make sure in the Device manager of the Microsoft Windows whether the device for displaying of live view has properly installed drivers e AV USB 2 0 Pro Device Sound video and game controllers ai AV USB 2 0 Pro e Leadtek WinFast VC100 XP Device 306 Troubleshooting FAQ Sound video and game controllers P WinFast YC100 WDM Audio Capture T WinFast YC100 WDM Crossbar P WinFast CLO0 WDM Video Capture Make sure the video output of the camera is connected to the device for live view displaying Into the yellow CINCH connector of the device for displaying of live view shou
305. ving newly acquired images a Save As dialog box will be displayed which will enable you to choose the location names and types of the files being saved If all selected images have already been saved this option will be inactive Option Save All Option File Save All saves all images displayed in the Thumbnail Browser If the images being saved have already been saved before they will be saved again into their original locations If the JPEG or TIFF types of images are being re saved into their original locations a Saving Parameters dialog box will be displayed Other types of images BMP a PNG will be re saved automatically If you are saving newly acquired images a Save As dialog box will be displayed which will enable you to choose the location names and types of files being saved If all images have already been saved this option will be inactive Saving Parameters Window Saving Parameters window will be displayed after one of these functions File Save or File Save All has been activated while re saving the JPEG or TIFF type images into their original locations 101 Main Program Window Saving Parameters x In this window parameters for saving of all images of the JPEG and TIFF types which will be saved to their original locations can be adjusted BMP and PNG images will be saved automatically JPEG and TIFF Images to Be Saved Flattening Layers Common Option for All Images in the List Keep Inser
306. w at all For this function 166 Main Program Window a folder selected as the default folder for image saving is set in the default state Active mode for automatic image saving will be indicated by the icon in the status bar in the right bottom corner of the program If you move the mouse over this icon information about the folder where images are being saved will be displayed In the Type of New Images part it is possible to select the type format which shall have the newly acquired images Original Type option preserves the image type which is provided by the active imaging device If you select one of the JPEG BMP TIFF or PNG types from the list all newly acquired images will be converted to the selected type This option is valid even for automatic image saving If automatic image saving is set you may set or change the folder into which the acquired images will be saved in the Folder for Automatic Image Saving part If the option Program Only is active in the Store Images after Acquisition in part it is possible to in the Numbering of Acquired Images part select the Always Begin Numbering from 00001 after Program Start up mode When this mode is active numbering of acquired images will begin with 00001 after each start of the QuickPHOTO INDUSTRIAL 3 0 program If you select the Build on Previous Numbering option numbering of acquired images will start from the last used number QuickPHOTO INDUSTRIAL 3 0 program wil
307. will see the effect of the shading correction function reflecting the current settings Using the slider you may set the level of correction interactively Zo Control Panels of Imaging Devices Using the Shading Correction Function Important Shading correction gives good results only if all the settings of the microscope and the camera are the same while acquiring images as when capturing a calibration mask If you change the conditions change of magnification change of illumination change of image quality and so on it is necessary to acquire the calibration mask again To use the Shading correction function it is necessary to create a calibration image the so called mask first Proceed as follows Transmitted Light l Focus the microscope using the desired microscope s magnification switch the camera s exposure mode to manual and manually set the exposure time 2 Remove the specimen and adjust shorten the exposure time so the inhomogeneities of the illumination are visible Optimal setting is such the center of the field of view is white and in the corners there are visible grey inhomogeneities The darkest areas should be grey not black 3 Click the Auto WB button automatic white balance on the Zmage tab 4 On the mage tab set the desired image quality 5 Switch to the Shading correction tab and select the Enabled option In the Light type part select the Transmitted option Then click the Capture m
308. with the same privileges as the QuickPHOTO INDUSTRIAL 3 0 program for example both programs with the standard user s privileges or both with administrator s privileges If each of the programs will be running with different privileges a warning message will be displayed and this function will not be activated If more workbooks are opened in the Microsoft Excel program or multiple instances of the Microsoft Excel program are running a dialog box with a list of available workbooks 201 Control Panels of Imaging Devices will be displayed during activation of this function Continuous exporting function will be activated after selection of one of the workbooks from the list Function for continuous export can be turned off by another clicking the Export Measured Values Continuously to Microsoft Excel a button Settings Click the Settings seo button to open a dialog box with the measurement settings Note Original values of all parameters of the Settings dialog box can be restored anytime by clicking the Restore Default Settings button Font Colors Tab Options x Main Lines of Measuring Objects Color D Change Thickness Dimension Lines and Leaders Color of Labels with Measured Values Transparent Custom Color Font of Measured Values Font Color Change Font Size Restore Default Settings OK Cancel 202 Control Panels of Imaging Device
309. ws the position where the timestamp will be inserted in the acquired images Timestamp Settings If you click the Options button or 1f you double click into the Orientation field a dialog box for setting of the timestamp will open 230 Control Panels of Imaging Devices Timestamp Settings Format Font Type Arial Font Color fj Black Timestamp Size 30 C Bold Background Color E White C Transparent Date Format dd mmiyyyy Position Orientation Top Left Top Right 2 Bottom Left Bottom Right Preview 16 10 2012 11 21 30 Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text Profiles Tab All settings of the control panel except for the settings of the Shading Correction function can be saved into one of the four profiles and at any time loaded again as Profiles led savetoProfie Save to led savetoProfie E 1 EE 2 E 3 cecal aut 4 Defaut Profle Defaut Profle Profile Profiles 231 Control Panels of Imaging Devices Save to Profile if you click this button a dialog box will be displayed for saving of the current control panel s
310. y Jue Contrast y 27 Y Gamma W 1 Preview Before Changes Default Values 88 Main Program Window Image Adjustment Brightness Contrast Gamma Red Green Blue E Red y eG E Green y 23 H Blue y A Preview Before Changes Preview After Changes Default Values Histogram Adjustment Option Image Adjust Histogram and the icon open a Histogram Adjustment dialog box Histogram Adjustment Channel Input Values Luminosity Scaling Color Channels T 205 Preview Before Changes Preview After Changes Default Values 89 Main Program Window This dialog box consists of several parts In the Channel part you can choose in which color channel of the image will the histogram adjustment take place Option Luminosity effects the whole image all color channels at the same time If you check the option Color Channels it 1s possible to adjust individual color channels independently In the Input Values part actual histogram adjustments take place Displayed graph represents a histogram for a channel selected in the Channel part Histogram scale can be changed using the vertical Scaling slider Under the histogram graph there are small triangles displayed on the edges These small triangles can be moved and thus set the border histogram values the so called black point left triangle and th
311. y selecting the corresponding type of measurement from the displayed menu In the Measure Arbitrary Angle amp mode by clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert the first arm of an angle Then by clicking the left mouse button insert the second arm of an angle If you want to create precisely horizontal or vertical arm of 121 Main Program Window an angle hold the Shift key while creating it Convex angle will be measured If you want to measure an angle of more than 180 drag with a mouse the label with the measured value to the second half plane In the Measure Angle from the X Axis or Measure Angle from the Y Axis mode by clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert the first arm of an angle This arm of an angle will automatically be completed with the corresponding axis and an angle defined in such a way will be calculated If you want to measure the side angle to the given axis drag with a mouse the label with the measured value to the second half plane In the Measure Angle Defined by 2 Line Segments Z mode by clicking and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the beginning and then the end point insert a line segment defining the first arm of an
312. y x Select the quality of images according to the desired output type rs Quality Low Quality Small File Size for E mail Medium Quality Medium File Size for Standard Printing High Quality Large File Size for High Quality Printing Warning If you insert several images into the report in high quality the resulting file can be up to several tens of megabytes large Main Program Window Continue by clicking the Continue button 6 Creating of a new report will begin You will be informed about the process by the progress bar displayed in the status bar of the program You may not work with the QuickPHOTO INDUSTRIAL 3 0 program while creating a report Duration time of creation of the report depends on the number and size of the images being inserted and on the performance of the used computer After a report has been created an option to open it in the Microsoft Word program will appear You will be able to continue with standard work with the document in the Microsoft Word program Note If you select more images in the Thumbnail Browser than the selected template can contain a reduced number of images will be inserted to the report Created report based on the sample pattern document example_1_image doc may look for example like this one 157 Main Program Window Name John Surname Smith Conditions 20 C Date 10 3 2012 Note Description Line Segment Length 1

3. Installation of the QuickPHOTO INDUSTRIAL 3.0

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